Experimental Adaptation of Wild-Type Canine Distemper Virus (CDV) to the Human Entry Receptor CD150
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{"title"=>"Experimental Adaptation of Wild-Type Canine Distemper Virus (CDV) to the Human Entry Receptor CD150", "type"=>"journal", "authors"=>[{"first_name"=>"Maria", "last_name"=>"Bieringer", "scopus_author_id"=>"55620216600"}, {"first_name"=>"Jung Woo", "last_name"=>"Han", "scopus_author_id"=>"55889044800"}, {"first_name"=>"Sabine", "last_name"=>"Kendl", "scopus_author_id"=>"34067689100"}, {"first_name"=>"Mojtaba", "last_name"=>"Khosravi", "scopus_author_id"=>"55621087300"}, {"first_name"=>"Philippe", "last_name"=>"Plattet", "scopus_author_id"=>"22635793200"}, {"first_name"=>"Jürgen", "last_name"=>"Schneider-Schaulies", "scopus_author_id"=>"7004724565"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"isbn"=>"1932-6203", "sgr"=>"84874898947", "pui"=>"368517995", "pmid"=>"23554862", "issn"=>"19326203", "scopus"=>"2-s2.0-84874898947", "doi"=>"10.1371/journal.pone.0057488"}, "id"=>"c4696dfc-1fe1-3293-9f96-675f69da5254", "abstract"=>"Canine distemper virus (CDV), a close relative of measles virus (MV), is widespread and well known for its broad host range. When the goal of measles eradication may be achieved, and when measles vaccination will be stopped, CDV might eventually cross the species barrier to humans and emerge as a new human pathogen. In order to get an impression how fast such alterations may occur, we characterized required adaptive mutations to the human entry receptors CD150 (SLAM) and nectin-4 as first step to infect human target cells. Recombinant wild-type CDV-A75/17(red) adapted quickly to growth in human H358 epithelial cells expressing human nectin-4. Sequencing of the viral attachment proteins (hemagglutinin, H, and fusion protein, F) genes revealed that no adaptive alteration was required to utilize human nectin-4. In contrast, the virus replicated only to low titres (10(2) pfu/ml) in Vero cells expressing human CD150 (Vero-hSLAM). After three passages using these cells virus was adapted to human CD150 and replicated to high titres (10(5) pfu/ml). Sequence analyses revealed that only one amino acid exchange in the H-protein at position 540 Asp→Gly (D540G) was required for functional adaptation to human CD150. Structural modelling suggests that the adaptive mutation D540G in H reflects the sequence alteration from canine to human CD150 at position 70 and 71 from Pro to Leu (P70L) and Gly to Glu (G71E), and compensates for the gain of a negative charge in the human CD150 molecule. Using this model system our data indicate that only a minimal alteration, in this case one adaptive mutation, is required for adaptation of CDV to the human entry receptors, and help to understand the molecular basis why this adaptive mutation occurs.", "link"=>"http://www.mendeley.com/research/experimental-adaptation-wildtype-canine-distemper-virus-cdv-human-entry-receptor-cd150", "reader_count"=>44, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>3, "Student > Doctoral Student"=>3, "Researcher"=>10, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>4, "Student > Master"=>5, "Other"=>1, "Student > Bachelor"=>6, "Professor"=>5}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>3, "Student > Doctoral Student"=>3, "Researcher"=>10, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>4, "Student > Master"=>5, "Other"=>1, "Student > Bachelor"=>6, "Professor"=>5}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Medicine and Dentistry"=>8, "Agricultural and Biological Sciences"=>27, "Veterinary Science and Veterinary Medicine"=>3, "Psychology"=>1, "Computer Science"=>1, "Immunology and Microbiology"=>3}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>8}, "Psychology"=>{"Psychology"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>27}, "Computer Science"=>{"Computer Science"=>1}, "Unspecified"=>{"Unspecified"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>3}}, "reader_count_by_country"=>{"United States"=>1, "United Kingdom"=>2, "Mexico"=>1}, "group_count"=>3}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/985018"], "description"=>"<p>H358 cells were infected with recombinant wild-type CDV-A75/17<sup>red</sup> at a MOI of 1.0 (<b>A</b>), incubated for the indicated times and pictures of the red autofluorescence taken under UV light. At 8 days post-infection (dpi) virus was prepared and titrated using Vero-cSLAM cells, and H358 cells infected with this virus at a MOI of 0.01 (<b>B</b>). After 8 dpi virus was again prepared and titrated using Vero-cSLAM cells, and H358 cells were infected with this virus at a MOI of 0.01 for indicated times (<b>C</b>). Photomicrographs of the autofluorescent virus-encoded tD tomato red were taken under UV light (bar = 100 µm).</p>", "links"=>[], "tags"=>["epithelial", "cells", "h358"], "article_id"=>650497, "categories"=>["Microbiology", "Biological Sciences", "Virology", "Infectious Diseases"], "users"=>["Maria Bieringer", "Jung Woo Han", "Sabine Kendl", "Mojtaba Khosravi", "Philippe Plattet", "Jürgen Schneider-Schaulies"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0057488.g001", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Infection_of_human_epithelial_cells_H358_with_CDV_A75_17_red_/650497", "title"=>"Infection of human epithelial cells H358 with CDV-A75/17<sup>red</sup>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-13 09:23:57"}
  • {"files"=>["https://ndownloader.figshare.com/files/985019"], "description"=>"<p>Human CD150-expressing Vero cells (Vero-hSLAM) were infected with recombinant wild-type CDV-A75/17<sup>red</sup> at a MOI of 1.0 (<b>A</b>), incubated for the indicated times and pictures of the red autofluorescence taken under UV light. At 4 days post-infection (dpi) virus was prepared and titrated, and Vero-hSLAM cells infected at a MOI of 0.01 (<b>B</b>). After 4 dpi virus was again prepared and titrated, and Vero-hSLAM cells were infected with this virus at a MOI of 0.01 for indicated times (<b>C</b>). Titres of the virus preparations were determined using Vero-cSLAM cells and are given in (<b>D</b>). Photomicrographs of the autofluorescent virus-encoded tD tomato red were taken under UV light (bar = 100 µm).</p>", "links"=>[], "tags"=>["vero-hslam", "cells"], "article_id"=>650498, "categories"=>["Microbiology", "Biological Sciences", "Virology", "Infectious Diseases"], "users"=>["Maria Bieringer", "Jung Woo Han", "Sabine Kendl", "Mojtaba Khosravi", "Philippe Plattet", "Jürgen Schneider-Schaulies"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0057488.g002", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Infection_of_Vero_hSLAM_cells_with_CDV_A75_17_red_/650498", "title"=>"Infection of Vero-hSLAM cells with CDV-A75/17<sup>red</sup>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-13 09:24:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/985022"], "description"=>"<p>Vero-hSLAM cells were infected in the absence and presence of increasing concentrations of human CD150-specific monoclonal antibody A12 (<b>A</b>; 1 and 5 µg/ml), or isotype control antibody (IgG1k; <b>B</b>; 1 and 5 µg/ml) for 2 days with human CD150-adapted CDV-A75/17<sup>red</sup> at a MOI of 0.1. As further control Vero cells expressing canine CD150, which is not recognized by mAb A12, were infected in the absence and presence of mAb A12 (<b>C</b>; 1 and 5 µg/ml). Photomicrographs of the autofluorescent virus-encoded tD tomato red were taken under UV light (bar = 100 µm).</p>", "links"=>[], "tags"=>["cd150-adapted", "inhibited", "cd150"], "article_id"=>650500, "categories"=>["Microbiology", "Biological Sciences", "Virology", "Infectious Diseases"], "users"=>["Maria Bieringer", "Jung Woo Han", "Sabine Kendl", "Mojtaba Khosravi", "Philippe Plattet", "Jürgen Schneider-Schaulies"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0057488.g003", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Infection_with_human_CD150_adapted_CDV_A75_17_red_is_specifically_inhibited_by_CD150_antibodies_/650500", "title"=>"Infection with human CD150-adapted CDV-A75/17<sup>red</sup> is specifically inhibited by CD150 antibodies.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-13 09:24:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/985023"], "description"=>"<p>Vero-cSLAM cells (open circles) and Vero-hSLAM cells (closed circles) were infected with parental CDV-A75/17<sup>red</sup>, and Vero-cSLAM cells (blue triangles) and Vero-hSLAM cells (red squares) were infected with human CD150-adapted CDV-A75/17<sup>red</sup> (CDV-A75-ad) at a MOI of 0.01 and incubated for indicated times before harvesting cell bound plus released virus. The viruses were then titrated using the optimal target cells for each virus (n = 3, using 3 independently adapted viruses).</p>", "links"=>[], "tags"=>["curves", "adapted", "canine", "cd150-expressing"], "article_id"=>650501, "categories"=>["Microbiology", "Biological Sciences", "Virology", "Infectious Diseases"], "users"=>["Maria Bieringer", "Jung Woo Han", "Sabine Kendl", "Mojtaba Khosravi", "Philippe Plattet", "Jürgen Schneider-Schaulies"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0057488.g004", "stats"=>{"downloads"=>6, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparison_of_single_step_growth_curves_of_parental_and_adapted_CDV_A75_17_red_using_canine_and_human_CD150_expressing_target_cells_/650501", "title"=>"Comparison of single step growth curves of parental and adapted CDV-A75/17<sup>red</sup> using canine and human CD150-expressing target cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-13 09:24:20"}
  • {"files"=>["https://ndownloader.figshare.com/files/985024"], "description"=>"<p>The receptor (CD150) binding site of the Morbillivirus H-proteins comprises approximately amino acid 500 to 550. CDV-H position 540 (corresponding to MV-H position 544) is located nearby the cognate receptor interacting residues 525, 526, and 529 <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057488#pone.0057488-Zipperle1\" target=\"_blank\">[25]</a>. CDV-A75-ad = human CD150-adapted CDV-A75/17<sup>red</sup>.</p>", "links"=>[], "tags"=>["cd150", "binding", "sites", "morbillivirus"], "article_id"=>650502, "categories"=>["Microbiology", "Biological Sciences", "Virology", "Infectious Diseases"], "users"=>["Maria Bieringer", "Jung Woo Han", "Sabine Kendl", "Mojtaba Khosravi", "Philippe Plattet", "Jürgen Schneider-Schaulies"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0057488.g005", "stats"=>{"downloads"=>2, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparison_of_the_CD150_binding_sites_of_Morbillivirus_H_proteins_/650502", "title"=>"Comparison of the CD150 binding sites of Morbillivirus H proteins.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-13 09:24:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/985026"], "description"=>"<p>Vero-cSLAM and Vero-hSLAM cells were transfected with plasmids expressing wild-type CDV-H<sub>A75/17</sub> and mutated H<sub>A75/17-D540G</sub> in combination with wild-type CDV-F as indicated, and incubated for 24 h before phase-contrast microphotographs were taken (bar = 100 µm).</p>", "links"=>[], "tags"=>["co-transfection", "plasmids", "expressing", "cdv-h", "-f"], "article_id"=>650504, "categories"=>["Microbiology", "Biological Sciences", "Virology", "Infectious Diseases"], "users"=>["Maria Bieringer", "Jung Woo Han", "Sabine Kendl", "Mojtaba Khosravi", "Philippe Plattet", "Jürgen Schneider-Schaulies"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0057488.g006", "stats"=>{"downloads"=>3, "page_views"=>29, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Syncytium_formation_after_co_transfection_of_plasmids_expressing_CDV_H_and_F_proteins_/650504", "title"=>"Syncytium formation after co-transfection of plasmids expressing CDV-H and -F proteins.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-13 09:24:34"}
  • {"files"=>["https://ndownloader.figshare.com/files/985028"], "description"=>"<p>Side (A) and top (B) view of the globular head of CDV-H (amino acids 188 to 602) presented in magenta with highlighted amino acid 540 G (green), and of the interacting first V-like domain of human CD150 (amino acids 32 to 140) shown in blue with highlighted amino acid 71 E (yellow). The structures were modelled based on the crystal structure of MV-H bound to CD150 <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057488#pone.0057488-Hashiguchi2\" target=\"_blank\">[33]</a>.</p>", "links"=>[], "tags"=>["cdv-h-cd150"], "article_id"=>650506, "categories"=>["Microbiology", "Biological Sciences", "Virology", "Infectious Diseases"], "users"=>["Maria Bieringer", "Jung Woo Han", "Sabine Kendl", "Mojtaba Khosravi", "Philippe Plattet", "Jürgen Schneider-Schaulies"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0057488.g007", "stats"=>{"downloads"=>4, "page_views"=>24, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Structural_model_of_the_CDV_H_CD150_interaction_/650506", "title"=>"Structural model of the CDV-H-CD150 interaction.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-13 09:24:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/985029"], "description"=>"<p>The MV-H binding site of CD150 was mapped between amino acid 61 and 131 <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057488#pone.0057488-Hashiguchi2\" target=\"_blank\">[33]</a>. Amino acid 71 is an uncharged G in dog CD150, and a negatively charged E in human CD150 (NM_001003084 <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057488#pone.0057488-Zipperle1\" target=\"_blank\">[25]</a>).</p>", "links"=>[], "tags"=>["canine", "cd150", "sequences", "morbillivirus", "binding"], "article_id"=>650507, "categories"=>["Microbiology", "Biological Sciences", "Virology", "Infectious Diseases"], "users"=>["Maria Bieringer", "Jung Woo Han", "Sabine Kendl", "Mojtaba Khosravi", "Philippe Plattet", "Jürgen Schneider-Schaulies"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0057488.g008", "stats"=>{"downloads"=>1, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparison_of_the_canine_and_human_CD150_sequences_around_the_Morbillivirus_H_binding_site_/650507", "title"=>"Comparison of the canine and human CD150 sequences around the Morbillivirus H binding site.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-13 09:24:46"}

PMC Usage Stats | Further Information

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Relative Metric

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