Probing the Interaction Forces of Prostate Cancer Cells with Collagen I and Bone Marrow Derived Stem Cells on the Single Cell Level
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{"title"=>"Probing the Interaction Forces of Prostate Cancer Cells with Collagen I and Bone Marrow Derived Stem Cells on the Single Cell Level", "type"=>"journal", "authors"=>[{"first_name"=>"Ediz", "last_name"=>"Sariisik", "scopus_author_id"=>"55614805900"}, {"first_name"=>"Denitsa", "last_name"=>"Docheva", "scopus_author_id"=>"16047307200"}, {"first_name"=>"Daniela", "last_name"=>"Padula", "scopus_author_id"=>"24074605500"}, {"first_name"=>"Cvetan", "last_name"=>"Popov", "scopus_author_id"=>"24436613800"}, {"first_name"=>"Jan", "last_name"=>"Opfer", "scopus_author_id"=>"55466695000"}, {"first_name"=>"Matthias", "last_name"=>"Schieker", "scopus_author_id"=>"8697780900"}, {"first_name"=>"Hauke", "last_name"=>"Clausen-Schaumann", "scopus_author_id"=>"6602852848"}, {"first_name"=>"Martin", "last_name"=>"Benoit", "scopus_author_id"=>"7103191726"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"isbn"=>"10.1371/journal.pone.0057706", "pmid"=>"23472100", "doi"=>"10.1371/journal.pone.0057706", "pui"=>"368465517", "issn"=>"19326203", "sgr"=>"84874591491", "scopus"=>"2-s2.0-84874591491"}, "id"=>"98a18caf-e7bb-31b7-9f4c-6a3bcea46434", "abstract"=>"Adhesion of metastasizing prostate carcinoma cells was quantified for two carcinoma model cell lines LNCaP (lymph node-specific) and PC3 (bone marrow-specific). By time-lapse microscopy and force spectroscopy we found PC3 cells to preferentially adhere to bone marrow-derived mesenchymal stem cells (SCP1 cell line). Using atomic force microscopy (AFM) based force spectroscopy, the mechanical pattern of the adhesion to SCP1 cells was characterized for both prostate cancer cell lines and compared to a substrate consisting of pure collagen type I. PC3 cells dissipated more energy (27.6 aJ) during the forced de-adhesion AFM experiments and showed significantly more adhesive and stronger bonds compared to LNCaP cells (20.1 aJ). The characteristic signatures of the detachment force traces revealed that, in contrast to the LNCaP cells, PC3 cells seem to utilize their filopodia in addition to establish adhesive bonds. Taken together, our study clearly demonstrates that PC3 cells have a superior adhesive affinity to bone marrow mesenchymal stem cells, compared to LNCaP. Semi-quantitative PCR on both prostate carcinoma cell lines revealed the expression of two Col-I binding integrin receptors, α1β1 and α2β1 in PC3 cells, suggesting their possible involvement in the specific interaction to the substrates. Further understanding of the exact mechanisms behind this phenomenon might lead to optimized therapeutic applications targeting the metastatic behavior of certain prostate cancer cells towards bone tissue.", "link"=>"http://www.mendeley.com/research/probing-interaction-forces-prostate-cancer-cells-collagen-i-bone-marrow-derived-stem-cells-single-ce", "reader_count"=>35, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Researcher"=>5, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>17, "Student > Postgraduate"=>2, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>2, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Researcher"=>5, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>17, "Student > Postgraduate"=>2, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>2, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>1, "Materials Science"=>2, "Agricultural and Biological Sciences"=>16, "Medicine and Dentistry"=>4, "Physics and Astronomy"=>7, "Chemistry"=>1, "Social Sciences"=>1, "Computer Science"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Materials Science"=>{"Materials Science"=>2}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Chemistry"=>{"Chemistry"=>1}, "Social Sciences"=>{"Social Sciences"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>7}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>16}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"United States"=>2, "France"=>1, "India"=>1}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/977762", "https://ndownloader.figshare.com/files/977763"], "description"=>"<div><p>Adhesion of metastasizing prostate carcinoma cells was quantified for two carcinoma model cell lines LNCaP (lymph node-specific) and PC3 (bone marrow-specific). By time-lapse microscopy and force spectroscopy we found PC3 cells to preferentially adhere to bone marrow-derived mesenchymal stem cells (SCP1 cell line). Using atomic force microscopy (AFM) based force spectroscopy, the mechanical pattern of the adhesion to SCP1 cells was characterized for both prostate cancer cell lines and compared to a substrate consisting of pure collagen type I. PC3 cells dissipated more energy (27.6 aJ) during the forced de-adhesion AFM experiments and showed significantly more adhesive and stronger bonds compared to LNCaP cells (20.1 aJ). The characteristic signatures of the detachment force traces revealed that, in contrast to the LNCaP cells, PC3 cells seem to utilize their filopodia in addition to establish adhesive bonds. Taken together, our study clearly demonstrates that PC3 cells have a superior adhesive affinity to bone marrow mesenchymal stem cells, compared to LNCaP. Semi-quantitative PCR on both prostate carcinoma cell lines revealed the expression of two Col-I binding integrin receptors, α1β1 and α2β1 in PC3 cells, suggesting their possible involvement in the specific interaction to the substrates. Further understanding of the exact mechanisms behind this phenomenon might lead to optimized therapeutic applications targeting the metastatic behavior of certain prostate cancer cells towards bone tissue.</p> </div>", "links"=>[], "tags"=>["probing", "forces", "prostate", "cancer", "cells", "collagen", "marrow", "derived", "level"], "article_id"=>644852, "categories"=>["Physics", "Biochemistry", "Cell Biology", "Developmental Biology", "Biophysics", "Cancer", "Physiology", "Chemistry"], "users"=>["Ediz Sariisik", "Denitsa Docheva", "Daniela Padula", "Cvetan Popov", "Jan Opfer", "Matthias Schieker", "Hauke Clausen-Schaumann", "Martin Benoit"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0057706.s001", "https://dx.doi.org/10.1371/journal.pone.0057706.s002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Probing_the_Interaction_Forces_of_Prostate_Cancer_Cells_with_Collagen_I_and_Bone_Marrow_Derived_Stem_Cells_on_the_Single_Cell_Level__/644852", "title"=>"Probing the Interaction Forces of Prostate Cancer Cells with Collagen I and Bone Marrow Derived Stem Cells on the Single Cell Level", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-03-06 09:22:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/978172"], "description"=>"<p>(<b>A</b>) Phase-contrast and fluorescent microscopy of CFDA-labelled PC3 and LNCaP cells plated on SCP1 monolayers in 6-well dishes. Images are taken after 4 h. (<b>B</b>) Quantification of adherent PC3 and LNCaP cells after 4 and 12 h cultivation on SCP1 monolayers. The percentage of adherent cells was quantified first, by manual counting of the CFDA-labelled cells with the cell counter tool in Image J software and second, by comparing to the initial number of plated cells (5×10<sup>5</sup> cells/well). In the images also a slight background of CFDA dye particles is visible (more apparent in the LNCaP image). The analyses revealed that already at 4 h PC3 cells completely adhered on SPC1 cells while LNCaP cells had a significantly lower adhesion rate at 4 h and 12 h. The graph bars show mean ± SD of four independent experiments (p<0.0001, unpaired t-test). (<b>C</b>) PC and LNCaP cells (2×10<sup>5</sup> cells/well) were grown on SCP1 monolayers in 6-well dishes for up to 8 days. Phase-contrast images demonstrated the formation and propagation of PC3 colonies (outlined) on the top of SCP1 cells between day 1 and 8. In contrast, LNCaP cells formed small cell clusters (arrows) that did not expand but rather regressed by day 8. (<b>D</b>) Quantification of PC and LNCaP cell numbers after 1, 5 and 8 days of cultivation on SCP1 monolayers. The proliferation of PC3 and LNCaP cells was calculated by subtracting the SCP1 control monolayers from the total cell count of the co-culture. Similarly to the microscopy data, the quantitative analysis confirmed that PC3 cells but not LNCaP were able to divide and further expand on SCP1 cells. The graph shows mean ± SD of three independent experiments for each time point (p<0.0001, unpaired t-test).</p>", "links"=>[], "tags"=>["adhesion", "pc3", "lncap", "cells", "scp1"], "article_id"=>645134, "categories"=>["Physics", "Biochemistry", "Cell Biology", "Developmental Biology", "Biophysics", "Cancer", "Physiology", "Chemistry"], "users"=>["Ediz Sariisik", "Denitsa Docheva", "Daniela Padula", "Cvetan Popov", "Jan Opfer", "Matthias Schieker", "Hauke Clausen-Schaumann", "Martin Benoit"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0057706.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cell_adhesion_and_expansion_of_PC3_and_LNCaP_cells_on_SCP1_monolayers_/645134", "title"=>"Cell adhesion and expansion of PC3 and LNCaP cells on SCP1 monolayers.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-06 13:43:46"}
  • {"files"=>["https://ndownloader.figshare.com/files/978177"], "description"=>"<p>(<b>A</b>) Phase contrast images of a prostate cancer cell attached to the cantilever (arrows) above an SCP1 monolayer (left) and a Col-I-coated slide (right). The scale bars indicate 10 µm. On the lower left corners immunofluorescence images are inserted. Col-I, labeled with AlexaFluor488 fluorescence dye appears in green and cell nuclei, stained with DAPI in blue. (<b>B</b>) Single cells from two different prostate cancer cell lines (PC3 and LNCaP) were immobilized to a tipless AFM cantilever (force sensor) in order to study their interaction forces with the apical surface of a SCP-1 monolayer (representing mesenchymal stem cells) or with Col-I (representing bone matrix). (C) Schematic top view of the culture dish lid with a BSA-coated glass cover slip (as substrate for fishing a gently injected prostate cancer cell) and a Col-I coated glass cover slip both on top of a monolayer of mesenchymal stem cells. For calibration and fishing a cell, the force sensor visits the BSA slide, for the experiment on collagen the Col-I slide and for the experiment on mesenchymal cells the SCP1 monolayer.</p>", "links"=>[], "tags"=>["chemistry", "cell biology", "physiology", "oncology", "biophysics", "developmental biology", "physics", "Biochemistry"], "article_id"=>645139, "categories"=>["Physics", "Biochemistry", "Cell Biology", "Developmental Biology", "Biophysics", "Cancer", "Physiology", "Chemistry"], "users"=>["Ediz Sariisik", "Denitsa Docheva", "Daniela Padula", "Cvetan Popov", "Jan Opfer", "Matthias Schieker", "Hauke Clausen-Schaumann", "Martin Benoit"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0057706.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Schematic_representation_of_the_experimental_setup_/645139", "title"=>"Schematic representation of the experimental setup.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-06 13:44:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/978181"], "description"=>"<p>The lowest data point to the left marks the contact force of 100 pN; the white dotted line represents the baseline intersecting the retrace curve at the black circle defining the cell surface; the black line is the de-noised signal and the red crosses indicate detected de-adhesion steps where the adhesion force evaluation takes place. (<b>A</b>) Force curve from a PC3-cell interacting with Col-I for illustrating the adhesion force evaluation: Red arrow #1: step height of the first de-adhesion event in the retraction curve. The detachment force is the absolute measure from the red cross down to the base line; #2: step height of the second de-adhesion event after a force plateau of 0.9 µm in length; #3: step position of the first de-adhesion event; #4: step position of the second de-adhesion event. (For definitions see <b>Cell Adhesion Force Evaluation</b> in the <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057706#s2\" target=\"_blank\">Materials and Methods</a> section). Characteristic curves from each of the four different types of experiments are represented: (<b>B</b>) PC3 on Col-I, (<b>C</b>) PC3 on SCP1 monolayer, (<b>D</b>) LNCaP on Col-I and (<b>E</b>) LNCaP on SCP1 monolayer.</p>", "links"=>[], "tags"=>["force-distance", "prostate", "cancer", "substrate", "retraction", "clarity", "shifted", "50", "pn"], "article_id"=>645143, "categories"=>["Physics", "Biochemistry", "Cell Biology", "Developmental Biology", "Biophysics", "Cancer", "Physiology", "Chemistry"], "users"=>["Ediz Sariisik", "Denitsa Docheva", "Daniela Padula", "Cvetan Popov", "Jan Opfer", "Matthias Schieker", "Hauke Clausen-Schaumann", "Martin Benoit"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0057706.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Representative_force_distance_curves_in_green_the_approach_of_the_force_sensor_with_a_prostate_cancer_cell_to_the_substrate_and_in_blue_the_retraction_for_clarity_the_blue_curve_is_shifted_by_approximately_50_pN_with_respect_to_the_green_curve_/645143", "title"=>"Representative force-distance curves: in green the approach of the force sensor with a prostate cancer cell to the substrate and in blue the retraction (for clarity the blue curve is shifted by approximately 50 pN with respect to the green curve).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-06 13:45:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/978187"], "description"=>"<p>(<b>A</b>) Percentages of force curves with at least one de-adhesion event. (<b>B</b>) Number of de-adhesion events within one adhesive curve. Error bars correspond to standard error of the mean. A significant p-value from an unpaired t-test of the PC3 data with respect to the LNCaP data is marked by *(p<0.05). The mean of each individual cell is given by a red cross. (<b>C</b>) Medians of the height of individual de-adhesion steps. (<b>D</b>) Medians of the position of these de-adhesion events. (E) Medians of the detachment force. (F) Medians of the work of detachment. Quartiles are indicated by double flags and the median of each individual cell is given by a red cross. Cell adhesion force data were acquired from 16 PC3 or 10 LNCaP cells interacting with Col-I (1485 and 760 force curves respectively), and 17 PC3 or 11 LNCaP cells interacting with SCP1 monolayers (1526 and 878 force curves respectively).</p>", "links"=>[], "tags"=>["adhesion", "afm", "spectroscopy", "pc", "cells", "col-i", "scp1"], "article_id"=>645149, "categories"=>["Physics", "Biochemistry", "Cell Biology", "Developmental Biology", "Biophysics", "Cancer", "Physiology", "Chemistry"], "users"=>["Ediz Sariisik", "Denitsa Docheva", "Daniela Padula", "Cvetan Popov", "Jan Opfer", "Matthias Schieker", "Hauke Clausen-Schaumann", "Martin Benoit"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0057706.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cell_adhesion_AFM_force_spectroscopy_measurements_of_PC_cells_with_Col_I_and_with_SCP1_monolayer_/645149", "title"=>"Cell adhesion AFM force spectroscopy measurements of PC cells with Col-I and with SCP1 monolayer.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-06 13:46:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/978191"], "description"=>"<p>Means of the percentage of individual de-adhesion steps representing the typical force pattern of filopodia-like steps (solid) and tether-like steps (striped) for the two cell lines PC3 and LNCaP. Each mean of a cell is represented by a red cross. Error bars correspond to standard error of the mean. A significant p-value from a t-test between the different steps within a prostate carcinoma cell line is indicated by *(p<0.05).</p>", "links"=>[], "tags"=>["filopodia-like", "steps", "tether-like", "cancer", "types", "scp1-monolayers", "600", "curves", "collagen-i", "500", "pc3", "54", "lncap", "bars", "cells", "indicated", "dashed"], "article_id"=>645153, "categories"=>["Physics", "Biochemistry", "Cell Biology", "Developmental Biology", "Biophysics", "Cancer", "Physiology", "Chemistry"], "users"=>["Ediz Sariisik", "Denitsa Docheva", "Daniela Padula", "Cvetan Popov", "Jan Opfer", "Matthias Schieker", "Hauke Clausen-Schaumann", "Martin Benoit"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0057706.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Analysis_of_filopodia_like_steps_versus_tether_like_steps_in_both_cancer_cell_types_to_A_SCP1_monolayers_from_more_than_600_force_curves_each_and_to_B_collagen_I_from_more_than_500_PC3_curves_but_only_54_LNCaP_curves_the_bars_for_the_LNCaP_cells_are_ther/645153", "title"=>"Analysis of filopodia-like steps versus tether-like steps in both cancer cell types to (A) SCP1-monolayers (from more than 600 force curves each) and to (B) collagen-I (from more than 500 PC3 curves but only 54 LNCaP curves; the bars for the LNCaP cells are therefore indicated by dashed lines).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-06 13:47:11"}
  • {"files"=>["https://ndownloader.figshare.com/files/978192"], "description"=>"<p>Semi-quantitative PCR for α1β1 and α2β1 integrins was performed with cDNA from PC3 and LNCaP cells and revealed a strong expression of both receptors in PC3 cells in comparison to LNCaP cells. The PCR results were reproduced independently three times.</p>", "links"=>[], "tags"=>["integrin"], "article_id"=>645154, "categories"=>["Physics", "Biochemistry", "Cell Biology", "Developmental Biology", "Biophysics", "Cancer", "Physiology", "Chemistry"], "users"=>["Ediz Sariisik", "Denitsa Docheva", "Daniela Padula", "Cvetan Popov", "Jan Opfer", "Matthias Schieker", "Hauke Clausen-Schaumann", "Martin Benoit"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0057706.g006"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Investigation_of_integrin_expression_/645154", "title"=>"Investigation of integrin expression.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-06 13:47:37"}

PMC Usage Stats | Further Information

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Relative Metric

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