Immunogenicity of Live Attenuated B. pertussis BPZE1 Producing the Universal Influenza Vaccine Candidate M2e
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{"title"=>"Immunogenicity of Live Attenuated B. pertussis BPZE1 Producing the Universal Influenza Vaccine Candidate M2e", "type"=>"journal", "authors"=>[{"first_name"=>"Hana", "last_name"=>"Kammoun", "scopus_author_id"=>"37026303900"}, {"first_name"=>"Xavier", "last_name"=>"Roux", "scopus_author_id"=>"36824959700"}, {"first_name"=>"Dominique", "last_name"=>"Raze", "scopus_author_id"=>"6602222535"}, {"first_name"=>"Anne Sophie", "last_name"=>"Debrie", "scopus_author_id"=>"6602948709"}, {"first_name"=>"Marina", "last_name"=>"de Filette", "scopus_author_id"=>"10440775300"}, {"first_name"=>"Tine", "last_name"=>"Ysenbaert", "scopus_author_id"=>"36614810600"}, {"first_name"=>"Nathalie", "last_name"=>"Mielcarek", "scopus_author_id"=>"6601947903"}, {"first_name"=>"Xavier", "last_name"=>"Saelens", "scopus_author_id"=>"6603597808"}, {"first_name"=>"Walter", "last_name"=>"Fiers", "scopus_author_id"=>"35593837000"}, {"first_name"=>"Camille", "last_name"=>"Locht", "scopus_author_id"=>"7006712654"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "doi"=>"10.1371/journal.pone.0059198", "pui"=>"368544516", "sgr"=>"84875121302", "pmid"=>"23555631", "scopus"=>"2-s2.0-84875121302"}, "id"=>"b218676d-3194-32b0-98cb-10ad7df846fa", "abstract"=>"BACKGROUND: Intranasal delivery of vaccines directed against respiratory pathogens is an attractive alternative to parenteral administration. However, using this delivery route for inactivated vaccines usually requires the use of potent mucosal adjuvants, and no such adjuvant has yet been approved for human use.\\n\\nMETHODOLOGY/PRINCIPAL FINDINGS: We have developed a live attenuated Bordetella pertussis vaccine, called BPZE1, and show here that it can be used to present the universal influenza virus epitope M2e to the mouse respiratory tract to prime for protective immunity against viral challenge. Three copies of M2e were genetically fused to the N-terminal domain of filamentous hemagglutinin (FHA) and produced in recombinant BPZE1 derivatives in the presence or absence of endogenous full-length FHA. Only in the absence of FHA intranasal administration of the recombinant BPZE1 derivative induced antibody responses to M2e and effectively primed BALB/c mice for protection against influenza virus-induced mortality and reduced the viral load after challenge. Strong M2e-specific antibody responses and protection were observed after a single nasal administration with the recombinant BPZE1 derivative, followed by a single administration of M2e linked to a virus-like particle without adjuvant, whereas priming alone with the vaccine strain did not protect.\\n\\nCONCLUSIONS/SIGNIFICANCE: Using recombinant FHA-3M2e-producing BPZE1 derivatives for priming and the universal influenza M2e peptide linked to virus-like particles for boosting may constitute a promising approach for needle-free and adjuvant-free nasal vaccination against influenza.", "link"=>"http://www.mendeley.com/research/immunogenicity-live-attenuated-b-pertussis-bpze1-producing-universal-influenza-vaccine-candidate-m2e", "reader_count"=>21, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Student > Doctoral Student"=>1, "Researcher"=>6, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>1, "Other"=>1, "Student > Master"=>2, "Student > Bachelor"=>2, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Student > Doctoral Student"=>1, "Researcher"=>6, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>1, "Other"=>1, "Student > Master"=>2, "Student > Bachelor"=>2, "Lecturer"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>11, "Medicine and Dentistry"=>3, "Immunology and Microbiology"=>3}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>11}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"France"=>1}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/991915"], "description"=>"<p>(A) BALB/c mice were i.n. infected with 10<sup>7 </sup>CFU of BPZE1 (black dots) or BPZM2e-ΔFHA (black squares), and the CFU in the lungs were quantified at the indicated time points. (B) BALB/c mice were i.n. immunized twice at a 4-week interval with 10<sup>7 </sup>CFU of the indicated strains. Sera were collected two weeks after the last immunization, and serum IgG responses to M2e were determined by an M2e-peptide ELISA. The results are expressed as means ± standard errors. ***, <i>p</i><0.001.</p>", "links"=>[], "tags"=>["immunogenicity"], "article_id"=>655725, "categories"=>["Infectious Diseases", "Immunology"], "users"=>["Hana Kammoun", "Xavier Roux", "Dominique Raze", "Anne-Sophie Debrie", "Marina De Filette", "Tine Ysenbaert", "Nathalie Mielcarek", "Xavier Saelens", "Walter Fiers", "Camille Locht"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0059198.g004", "stats"=>{"downloads"=>2, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Colonization_capacity_and_immunogenicity_of_BPZM2e_916_FHA_/655725", "title"=>"Colonization capacity and immunogenicity of BPZM2e-ΔFHA.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-20 12:10:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/991914"], "description"=>"<p>Unconcentrated culture supernatants of the indicated strains were subjected to SDS-PAGE and Coomassie-blue staining (A) and Immunoblot analysis (B) using a monoclonal antibody to M2e. The molecular weight markers and their sizes in kDa are indicated on the left.</p>", "links"=>[], "tags"=>["immunoblot", "supernatants", "bpzm2e"], "article_id"=>655724, "categories"=>["Infectious Diseases", "Immunology"], "users"=>["Hana Kammoun", "Xavier Roux", "Dominique Raze", "Anne-Sophie Debrie", "Marina De Filette", "Tine Ysenbaert", "Nathalie Mielcarek", "Xavier Saelens", "Walter Fiers", "Camille Locht"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0059198.g003", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_SDS_PAGE_and_Immunoblot_analyses_of_culture_supernatants_from_BPZM2e_and_BPZM2e_916_FHA_/655724", "title"=>"SDS-PAGE and Immunoblot analyses of culture supernatants from BPZM2e and BPZM2e-ΔFHA.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-20 12:10:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/991911"], "description"=>"<p>Immunoblot analysis of BPZM2e.</p>", "links"=>[], "tags"=>["immunology", "Infectious diseases"], "article_id"=>655721, "categories"=>["Infectious Diseases", "Immunology"], "users"=>["Hana Kammoun", "Xavier Roux", "Dominique Raze", "Anne-Sophie Debrie", "Marina De Filette", "Tine Ysenbaert", "Nathalie Mielcarek", "Xavier Saelens", "Walter Fiers", "Camille Locht"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0059198.g001", "stats"=>{"downloads"=>1, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Immunoblot_analysis_of_BPZM2e_/655721", "title"=>"Immunoblot analysis of BPZM2e.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-20 12:09:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/991918"], "description"=>"<p>Groups of 5 BALB/c mice were i.n. immunized with 10<sup>5 </sup>CFU of BPZE1 or BPZM2e-ΔFHA and challenged 4 weeks later with 10<sup>6 </sup>CFU of BPSM. Seven days after infection, the mice were sacrificed and bacterial loads in the lungs were determined and are expressed as CFU/lung. The results are expressed as means ± standard errors. ***, <i>p</i><0.001.</p>", "links"=>[], "tags"=>["immunology", "Infectious diseases"], "article_id"=>655727, "categories"=>["Infectious Diseases", "Immunology"], "users"=>["Hana Kammoun", "Xavier Roux", "Dominique Raze", "Anne-Sophie Debrie", "Marina De Filette", "Tine Ysenbaert", "Nathalie Mielcarek", "Xavier Saelens", "Walter Fiers", "Camille Locht"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0059198.g006", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Protection_against_B_pertussis_challenge_/655727", "title"=>"Protection against <i>B. pertussis</i> challenge.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-20 12:11:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1013018"], "description"=>"<p><i>B. pertussis</i> strains used in this study.</p>", "links"=>[], "tags"=>["strains"], "article_id"=>673424, "categories"=>["Infectious Diseases", "Immunology"], "users"=>["Hana Kammoun", "Xavier Roux", "Dominique Raze", "Anne-Sophie Debrie", "Marina De Filette", "Tine Ysenbaert", "Nathalie Mielcarek", "Xavier Saelens", "Walter Fiers", "Camille Locht"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0059198.t001", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_B_pertussis_strains_used_in_this_study_/673424", "title"=>"<i>B. pertussis</i> strains used in this study.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-03-19 00:57:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/991916"], "description"=>"<p>BALB/c mice were i.n. immunized with 10<sup>7 </sup>CFU of BPZE1 or BPZM2e-ΔFHA as indicated and boosted i.n. 4 weeks later with 10 µg M2e-VLP. Negative control mice received PBS. Sera were collected 2 weeks after the last immunization, and serum IgG responses to M2e were determined by an M2e-peptide ELISA (A). IgG1 and IgG2a ratios (B) were determined for the groups primed with BPZE1 or BPZM2e-ΔFHA and boosted with M2e-VLP. (C) Groups of 3 BALB/c mice were i.n. primed with 10<sup>7 </sup>CFU of BPZE1 or BPZM2e-ΔFHA and boosted 4 weeks later with 10 µg M2e-VLP. Two weeks after the last immunization the mice were challenged with 4 LD<sub>50</sub> of influenza A virus, and the viral loads in the lungs were measured at 4 days post challenge by TCID<sub>50</sub> quantification. The results are expressed as means of log10± standard errors. (D) Groups of 9 BALB/c mice were i.n. primed with 10<sup>7 </sup>CFU of BPZE1 or BPZM2e-ΔFHA and boosted 4 weeks later with 10 µg M2e-VLP. Two weeks after the last immunization the mice were challenged with 4 LD<sub>50</sub> of influenza A virus, and the percent of survival after challenge was monitored for up to 2 weeks. *, <i>p</i><0.05; ns, not significant.</p>", "links"=>[], "tags"=>["igg", "responses", "viral", "heterologous"], "article_id"=>655726, "categories"=>["Infectious Diseases", "Immunology"], "users"=>["Hana Kammoun", "Xavier Roux", "Dominique Raze", "Anne-Sophie Debrie", "Marina De Filette", "Tine Ysenbaert", "Nathalie Mielcarek", "Xavier Saelens", "Walter Fiers", "Camille Locht"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0059198.g005", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Anti_M2e_IgG_responses_and_protection_against_viral_challenge_after_heterologous_prime_boost_vaccination_/655726", "title"=>"Anti-M2e IgG responses and protection against viral challenge after heterologous prime/boost vaccination.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-20 12:10:54"}
  • {"files"=>["https://ndownloader.figshare.com/files/991913"], "description"=>"<p>(A) The growth curves of BPZE1 (closed circles) and BPZM2e (closed squares) in Stainer Scholte medium were determined by OD measurements at 600 nm over the indicated periods of time. (B) BALB/c mice were inoculated i.n. with 10<sup>6 </sup>CFU of BPZE1 or recombinant BPZM2e. Bacterial colonization of the lungs by BPZE1 (closed circles) and BPZM2e (closed squares) was measured in CFU over the indicated periods of time following i.n. administration. (C) BALB/c mice were i.n. immunized twice at a 4-week interval with 10<sup>7 </sup>CFU of BPZE1 or recombinant BPZM2e as indicated. Sera were collected at indicated time points after the first immunization, and serum IgG responses to M2e were determined by an M2e-peptide ELISA.</p>", "links"=>[], "tags"=>["colonization", "immunogenicity"], "article_id"=>655723, "categories"=>["Infectious Diseases", "Immunology"], "users"=>["Hana Kammoun", "Xavier Roux", "Dominique Raze", "Anne-Sophie Debrie", "Marina De Filette", "Tine Ysenbaert", "Nathalie Mielcarek", "Xavier Saelens", "Walter Fiers", "Camille Locht"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0059198.g002", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Growth_curves_colonization_capacity_and_immunogenicity_of_BPZM2e_/655723", "title"=>"Growth curves, colonization capacity and immunogenicity of BPZM2e.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-03-20 12:09:54"}

PMC Usage Stats | Further Information

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  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[269, 466, 588, 697, 800, 896, 988, 1076, 1165, 1254, 1340, 1417]}, {"subject_area"=>"/Biology and life sciences/Microbiology", "average_usage"=>[293, 503, 638, 755, 861, 960, 1056, 1146, 1239, 1323, 1403, 1491, 1568]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[264, 460, 584, 692, 794, 887, 978, 1067, 1154, 1241, 1328, 1408, 1474]}, {"subject_area"=>"/Medicine and health sciences/Infectious diseases", "average_usage"=>[297, 523, 655, 765, 866, 971, 1070, 1159, 1256, 1337, 1424, 1496, 1568]}, {"subject_area"=>"/Medicine and health sciences/Pathology and laboratory medicine", "average_usage"=>[267, 466, 592, 709, 806, 901, 989, 1075, 1162, 1254, 1342, 1424, 1486]}, {"subject_area"=>"/Medicine and health sciences/Public and occupational health", "average_usage"=>[291, 491, 610, 723, 817, 906, 991, 1071, 1153, 1238, 1324, 1411, 1478]}]}
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