Genetic Diversity and Distribution of the Ciguatera-Causing Dinoflagellate Gambierdiscus spp. (Dinophyceae) in Coastal Areas of Japan
Publication Date
April 11, 2013
Journal
PLoS ONE
Authors
Tomohiro Nishimura, Shinya Sato, Wittaya Tawong, Hiroshi Sakanari, et al
Volume
8
Issue
4
Pages
e60882
DOI
https://dx.plos.org/10.1371/journal.pone.0060882
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0060882
Web of Science
000317383200024
Scopus
84876153860
Mendeley
http://www.mendeley.com/research/genetic-diversity-distribution-ciguateracausing-dinoflagellate-gambierdiscus-spp-dinophyceae-coastal
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Genetic Diversity and Distribution of the Ciguatera-Causing Dinoflagellate Gambierdiscus spp. (Dinophyceae) in Coastal Areas of Japan", "type"=>"journal", "authors"=>[{"first_name"=>"Tomohiro", "last_name"=>"Nishimura", "scopus_author_id"=>"54789965600"}, {"first_name"=>"Shinya", "last_name"=>"Sato", "scopus_author_id"=>"55725611200"}, {"first_name"=>"Wittaya", "last_name"=>"Tawong", "scopus_author_id"=>"54414943900"}, {"first_name"=>"Hiroshi", "last_name"=>"Sakanari", "scopus_author_id"=>"54414930300"}, {"first_name"=>"Keita", "last_name"=>"Uehara", "scopus_author_id"=>"54790301600"}, {"first_name"=>"Md Mahfuzur Rahman", "last_name"=>"Shah", "scopus_author_id"=>"55785784300"}, {"first_name"=>"Shoichiro", "last_name"=>"Suda", "scopus_author_id"=>"7103184526"}, {"first_name"=>"Takeshi", "last_name"=>"Yasumoto", "scopus_author_id"=>"7103296383"}, {"first_name"=>"Yohsuke", "last_name"=>"Taira", "scopus_author_id"=>"55649852300"}, {"first_name"=>"Haruo", "last_name"=>"Yamaguchi", "scopus_author_id"=>"16235067900"}, {"first_name"=>"Masao", "last_name"=>"Adachi", "scopus_author_id"=>"7401545667"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84876153860", "doi"=>"10.1371/journal.pone.0060882", "sgr"=>"84876153860", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"23593339", "issn"=>"19326203", "pui"=>"368717486"}, "id"=>"86bc2464-cbce-332e-bf07-47be592afe2b", "abstract"=>"BACKGROUND: The marine epiphytic dinoflagellate genus Gambierdiscus produce toxins that cause ciguatera fish poisoning (CFP): one of the most significant seafood-borne illnesses associated with fish consumption worldwide. So far, occurrences of CFP incidents in Japan have been mainly reported in subtropical areas. A previous phylogeographic study of Japanese Gambierdiscus revealed the existence of two distinct phylotypes: Gambierdiscus sp. type 1 from subtropical and Gambierdiscus sp. type 2 from temperate areas. However, details of the genetic diversity and distribution for Japanese Gambierdiscus are still unclear, because a comprehensive investigation has not been conducted yet.\\n\\nMETHODS/PRINCIPAL FINDING: A total of 248 strains were examined from samples mainly collected from western and southern coastal areas of Japan during 2006-2011. The SSU rDNA, the LSU rDNA D8-D10 and the ITS region were selected as genetic markers and phylogenetic analyses were conducted. The genetic diversity of Japanese Gambierdiscus was high since five species/phylotypes were detected: including two reported phylotypes (Gambierdiscus sp. type 1 and Gambierdiscus sp. type 2), two species of Gambierdiscus (G. australes and G. cf. yasumotoi) and a hitherto unreported phylotype Gambierdiscus sp. type 3. The distributions of type 3 and G. cf. yasumotoi were restricted to the temperate and the subtropical area, respectively. On the other hand, type 1, type 2 and G. australes occurred from the subtropical to the temperate area, with a tendency that type 1 and G. australes were dominant in the subtropical area, whereas type 2 was dominant in the temperate area. By using mouse bioassay, type 1, type 3 and G. australes exhibited mouse toxicities.\\n\\nCONCLUSIONS/SIGNIFICANCE: This study revealed a surprising diversity of Japanese Gambierdiscus and the distribution of five species/phylotypes displayed clear geographical patterns in Japanese coastal areas. The SSU rDNA and the LSU rDNA D8-D10 as genetic markers are recommended for further use.", "link"=>"http://www.mendeley.com/research/genetic-diversity-distribution-ciguateracausing-dinoflagellate-gambierdiscus-spp-dinophyceae-coastal", "reader_count"=>42, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>4, "Researcher"=>10, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>9, "Student > Master"=>6, "Other"=>2, "Student > Bachelor"=>8, "Professor"=>2}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>4, "Researcher"=>10, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>9, "Student > Master"=>6, "Other"=>2, "Student > Bachelor"=>8, "Professor"=>2}, "reader_count_by_subject_area"=>{"Environmental Science"=>11, "Biochemistry, Genetics and Molecular Biology"=>4, "Agricultural and Biological Sciences"=>21, "Medicine and Dentistry"=>2, "Chemistry"=>1, "Earth and Planetary Sciences"=>2, "Energy"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Chemistry"=>{"Chemistry"=>1}, "Energy"=>{"Energy"=>1}, "Earth and Planetary Sciences"=>{"Earth and Planetary Sciences"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>21}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}, "Environmental Science"=>{"Environmental Science"=>11}}, "reader_count_by_country"=>{"Ireland"=>1, "Brazil"=>1, "Italy"=>1, "Germany"=>1, "French Polynesia"=>1}, "group_count"=>2}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84876153860"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84876153860?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84876153860&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84876153860&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84876153860", "dc:identifier"=>"SCOPUS_ID:84876153860", "eid"=>"2-s2.0-84876153860", "dc:title"=>"Genetic Diversity and Distribution of the Ciguatera-Causing Dinoflagellate Gambierdiscus spp. (Dinophyceae) in Coastal Areas of Japan", "dc:creator"=>"Nishimura T.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"8", "prism:issueIdentifier"=>"4", "prism:pageRange"=>nil, "prism:coverDate"=>"2013-04-11", "prism:coverDisplayDate"=>"11 April 2013", "prism:doi"=>"10.1371/journal.pone.0060882", "citedby-count"=>"58", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Kochi University", "affiliation-city"=>"Kochi", "affiliation-country"=>"Japan"}, {"@_fa"=>"true", "affilname"=>"Ehime University", "affiliation-city"=>"Matsuyama", "affiliation-country"=>"Japan"}], "pubmed-id"=>"23593339", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e60882", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0060882", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Journal Comments | Further Information

Twitter

Counter

  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"64", "xml_views"=>"4", "html_views"=>"480"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"34", "xml_views"=>"3", "html_views"=>"221"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"131"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"157"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"75"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"19", "xml_views"=>"3", "html_views"=>"116"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"61"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"114"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"30", "xml_views"=>"1", "html_views"=>"84"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"86"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"3", "html_views"=>"87"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"96"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"89"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"71"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"90"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"2", "html_views"=>"75"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"107"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"76"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"53"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"46"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"2", "html_views"=>"49"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"36"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"3", "html_views"=>"46"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"65"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"50"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"19", "xml_views"=>"1", "html_views"=>"53"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"2", "html_views"=>"50"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"83"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"40"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"23"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"2", "html_views"=>"14"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"4", "html_views"=>"23"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"2", "html_views"=>"12"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"9"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"35"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"26", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"14"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1021632"], "description"=>"<p>Nodal supports are of Bayesian posterior probability (pp) and Bootstrap (bt) values obtained by BI analysis and Maximum likelihood (ML) analysis, respectively. Nodes with strong supports (pp/bt  = 1.00/100) are shown as thick lines. For sequences obtained via cloning, a variant ID, starting with C, is shown followed by strain ID (i.e. KW070922_1_C4). Sequences obtained in the present study are indicated in color. A moderately supported branch (1.00/84) indicated by an asterisk consists of the temperate strains, whereas the others in <i>Gambierdiscus</i> sp. type 1 are all of the subtropical origin.</p>", "links"=>[], "tags"=>["Computational biology", "population genetics", "Genetic drift", "Sequence analysis", "Evolutionary biology", "Evolutionary systematics", "phylogenetics", "Model organisms", "Animal models", "mouse", "marine and aquatic sciences", "toxicology", "Veterinary toxicology", "inference", "phylogeny", "ssu", "rdna"], "article_id"=>680257, "categories"=>["Medicine", "Biological Sciences", "Earth and Environmental Sciences"], "users"=>["Tomohiro Nishimura", "Shinya Sato", "Wittaya Tawong", "Hiroshi Sakanari", "Keita Uehara", "Md Mahfuzur Rahman Shah", "Shoichiro Suda", "Takeshi Yasumoto", "Yohsuke Taira", "Haruo Yamaguchi", "Masao Adachi"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0060882.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Bayesian_inference_BI_phylogeny_of_the_SSU_rDNA_of_Gambierdiscus_species_phylotypes_/680257", "title"=>"Bayesian inference (BI) phylogeny of the SSU rDNA of <i>Gambierdiscus</i> species/phylotypes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-11 00:04:17"}
  • {"files"=>["https://ndownloader.figshare.com/files/1021633"], "description"=>"<p>Nodal supports are of Bayesian posterior probability (pp) and Bootstrap (bt) values obtained by BI analysis and Maximum likelihood (ML) analysis, respectively. Nodes with strong supports (pp/bt  = 1.00/100) are shown as thick lines. For sequences obtained via cloning, a variant ID, starting with C, is shown followed by strain ID (i.e. T080908_1_C1). Sequences obtained in the present study are indicated in color.</p>", "links"=>[], "tags"=>["Computational biology", "population genetics", "Genetic drift", "Sequence analysis", "Evolutionary biology", "Evolutionary systematics", "phylogenetics", "Model organisms", "Animal models", "mouse", "marine and aquatic sciences", "toxicology", "Veterinary toxicology", "inference", "phylogeny", "lsu", "rdna"], "article_id"=>680258, "categories"=>["Medicine", "Biological Sciences", "Earth and Environmental Sciences"], "users"=>["Tomohiro Nishimura", "Shinya Sato", "Wittaya Tawong", "Hiroshi Sakanari", "Keita Uehara", "Md Mahfuzur Rahman Shah", "Shoichiro Suda", "Takeshi Yasumoto", "Yohsuke Taira", "Haruo Yamaguchi", "Masao Adachi"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0060882.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Bayesian_inference_BI_phylogeny_of_the_D8_D10_region_of_the_LSU_rDNA_of_Gambierdiscus_species_phylotypes_/680258", "title"=>"Bayesian inference (BI) phylogeny of the D8–D10 region of the LSU rDNA of <i>Gambierdiscus</i> species/phylotypes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-11 00:04:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/1021634"], "description"=>"<p>Location of each sampling station and its ID as well as presence (yellow circle) or absence (gray circle) of <i>Gambierdiscus</i> cells in the sample is shown. Each island is marked as A: Hokkaido, B: Honshu (main Isl.), C: Shikoku, D: Kyushu, E: Okinawa Island, F: Miyako Island, Ikema Island and Irabu Island (from right to left), G: Ishigaki Island and Iriomote Island (from right to left). A: the boreal area, B, C and D: the temperate area, E, F and G: the subtropical area.</p>", "links"=>[], "tags"=>["Computational biology", "population genetics", "Genetic drift", "Sequence analysis", "Evolutionary biology", "Evolutionary systematics", "phylogenetics", "Model organisms", "Animal models", "mouse", "marine and aquatic sciences", "toxicology", "Veterinary toxicology"], "article_id"=>680259, "categories"=>["Medicine", "Biological Sciences", "Earth and Environmental Sciences"], "users"=>["Tomohiro Nishimura", "Shinya Sato", "Wittaya Tawong", "Hiroshi Sakanari", "Keita Uehara", "Md Mahfuzur Rahman Shah", "Shoichiro Suda", "Takeshi Yasumoto", "Yohsuke Taira", "Haruo Yamaguchi", "Masao Adachi"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0060882.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Map_of_research_area_/680259", "title"=>"Map of research area.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-11 00:04:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1021636"], "description"=>"<p>Enlargement of a part enclosed by an open square in Fig. 3. The numbers in each pie indicate the number of strains used for phylotyping. Each color in pies corresponds to a species/phylotype in phylogenetic trees in Figs. 1 and 2, i.e. red: <i>Gambierdiscus</i> sp. type 1, blue: <i>Gambierdiscus</i> sp. type 2, orange: <i>Gambierdiscus</i> sp. type 3, purple: <i>G. australes</i>, green: <i>G</i>. cf. <i>yasumotoi</i>. Each island is marked as B: Honshu (main Isl.), C: Shikoku, D: Kyushu, E: Okinawa Island, F: Miyako Island, Ikema Island and Irabu Island (from right to left), G: Ishigaki Island and Iriomote Island (from right to left). B, C and D: the temperate area, E, F and G: the subtropical area. <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060882#pone-0060882-g004\" target=\"_blank\">Figure 4A:</a> Total number of strains and composition of each species/phylotype. <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060882#pone-0060882-g004\" target=\"_blank\">Figure 4B:</a> Breakdown of species/phylotype composition from each sampling station.</p>", "links"=>[], "tags"=>["Computational biology", "population genetics", "Genetic drift", "Sequence analysis", "Evolutionary biology", "Evolutionary systematics", "phylogenetics", "Model organisms", "Animal models", "mouse", "marine and aquatic sciences", "toxicology", "Veterinary toxicology"], "article_id"=>680261, "categories"=>["Medicine", "Biological Sciences", "Earth and Environmental Sciences"], "users"=>["Tomohiro Nishimura", "Shinya Sato", "Wittaya Tawong", "Hiroshi Sakanari", "Keita Uehara", "Md Mahfuzur Rahman Shah", "Shoichiro Suda", "Takeshi Yasumoto", "Yohsuke Taira", "Haruo Yamaguchi", "Masao Adachi"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0060882.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Geographic_distribution_of_Gambierdiscus_species_phylotypes_/680261", "title"=>"Geographic distribution of <i>Gambierdiscus</i> species/phylotypes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-11 00:04:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/1021637"], "description"=>"*<p>: Annealing site in the ITS1-5.8S-ITS2 rDNA sequence of <i>Gambierdiscus yasumotoi</i> GYASU, GU968498 (Vandersea et al., 2010 Direct Submission).</p>**<p>: Annealing site in the LSU rDNA sequence of <i>Prorocentrum micans</i>, X16108 (Lenaers et al., 1989 <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060882#pone.0060882-Lenaers1\" target=\"_blank\">[51]</a>).</p>***<p>: Annealing site in the SSU rDNA sequence of <i>Pfiesteria piscicida</i>, AY245693 (Litaker et al., 2003 <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060882#pone.0060882-Litaker4\" target=\"_blank\">[44]</a>).</p>", "links"=>[], "tags"=>["Computational biology", "population genetics", "Genetic drift", "Sequence analysis", "Evolutionary biology", "Evolutionary systematics", "phylogenetics", "Model organisms", "Animal models", "mouse", "marine and aquatic sciences", "toxicology", "Veterinary toxicology", "primers", "pcr", "amplification", "dna"], "article_id"=>680262, "categories"=>["Medicine", "Biological Sciences", "Earth and Environmental Sciences"], "users"=>["Tomohiro Nishimura", "Shinya Sato", "Wittaya Tawong", "Hiroshi Sakanari", "Keita Uehara", "Md Mahfuzur Rahman Shah", "Shoichiro Suda", "Takeshi Yasumoto", "Yohsuke Taira", "Haruo Yamaguchi", "Masao Adachi"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0060882.t004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Oligonucleotide_primers_used_for_PCR_amplification_and_DNA_sequencing_/680262", "title"=>"Oligonucleotide primers used for PCR amplification and DNA sequencing.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-04-11 00:04:22"}
  • {"files"=>["https://ndownloader.figshare.com/files/1021638"], "description"=>"<p>bp*:Nucleotide bases of the SSU rDNA used for calculations.</p><p>n**: Numbers of species (species/phylotypes) used for calculations.</p><p>Reference***: Each ref. calculated molecular similarity (we converted values to uncorrected <i>p</i> distance) of individual SSU rDNA.</p><p>NC****: Not calculated.</p><p>2 orders*****: The values include between-species data set for 2 orders; Thalassiosirales and Naviculales.</p>", "links"=>[], "tags"=>["Computational biology", "population genetics", "Genetic drift", "Sequence analysis", "Evolutionary biology", "Evolutionary systematics", "phylogenetics", "Model organisms", "Animal models", "mouse", "marine and aquatic sciences", "toxicology", "Veterinary toxicology", "distances", "ssu", "rdna", "calculated", "uncorrected", "66", "sequences", "constructed", "cloned"], "article_id"=>680263, "categories"=>["Medicine", "Biological Sciences", "Earth and Environmental Sciences"], "users"=>["Tomohiro Nishimura", "Shinya Sato", "Wittaya Tawong", "Hiroshi Sakanari", "Keita Uehara", "Md Mahfuzur Rahman Shah", "Shoichiro Suda", "Takeshi Yasumoto", "Yohsuke Taira", "Haruo Yamaguchi", "Masao Adachi"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0060882.t001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Summary_of_genetic_distances_of_the_SSU_rDNA_over_the_p_distance_calculated_by_uncorrected_genetic_distance_UGD_model_among_and_within_66_consensus_sequences__66_strains_each_consensus_sequence_was_constructed_with_cloned_sequences_and_or_directly_sequen/680263", "title"=>"Summary of genetic distances of the SSU rDNA over the p distance calculated by uncorrected genetic distance (UGD) model among and within 66 consensus sequences ( = 66 strains; each consensus sequence was constructed with cloned sequences and/or directly sequence of each strain) of <i>Gambierdiscus</i> species/phylotypes, and other protists.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-04-11 00:04:23"}
  • {"files"=>["https://ndownloader.figshare.com/files/1021639"], "description"=>"<p>ND*: No data.</p>", "links"=>[], "tags"=>["Computational biology", "population genetics", "Genetic drift", "Sequence analysis", "Evolutionary biology", "Evolutionary systematics", "phylogenetics", "Model organisms", "Animal models", "mouse", "marine and aquatic sciences", "toxicology", "Veterinary toxicology", "sampling", "stations"], "article_id"=>680264, "categories"=>["Medicine", "Biological Sciences", "Earth and Environmental Sciences"], "users"=>["Tomohiro Nishimura", "Shinya Sato", "Wittaya Tawong", "Hiroshi Sakanari", "Keita Uehara", "Md Mahfuzur Rahman Shah", "Shoichiro Suda", "Takeshi Yasumoto", "Yohsuke Taira", "Haruo Yamaguchi", "Masao Adachi"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0060882.t002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Details_of_sampling_stations_where_each_Gambierdiscus_species_phylotype_was_observed_/680264", "title"=>"Details of sampling stations where each <i>Gambierdiscus</i> species/phylotype was observed.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-04-11 00:04:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/1021640"], "description"=>"<p>DSF*: Dichloromethane soluble fraction (DSF) toxicities correspond to CTXs toxicity.</p><p>MSF**: Aqueous methanol soluble fractions (MSF) toxicities correspond to MTXs toxicity.</p><p>―***: Not detected.</p><p>ND****: Not done.</p>", "links"=>[], "tags"=>["Computational biology", "population genetics", "Genetic drift", "Sequence analysis", "Evolutionary biology", "Evolutionary systematics", "phylogenetics", "Model organisms", "Animal models", "mouse", "marine and aquatic sciences", "toxicology", "Veterinary toxicology", "japanese", "tested", "mu", "defined", "20-g", "24"], "article_id"=>680265, "categories"=>["Medicine", "Biological Sciences", "Earth and Environmental Sciences"], "users"=>["Tomohiro Nishimura", "Shinya Sato", "Wittaya Tawong", "Hiroshi Sakanari", "Keita Uehara", "Md Mahfuzur Rahman Shah", "Shoichiro Suda", "Takeshi Yasumoto", "Yohsuke Taira", "Haruo Yamaguchi", "Masao Adachi"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0060882.t003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Toxicities_of_Japanese_Gambierdiscus_species_phylotypes_tested_by_a_mouse_bioassay_One_MU_is_defined_as_the_LD_50_dose_for_a_20_g_mouse_over_24_h_/680265", "title"=>"Toxicities of Japanese <i>Gambierdiscus</i> species/phylotypes tested by a mouse bioassay. One MU is defined as the LD<sub>50</sub> dose for a 20-g mouse over 24 h.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-04-11 00:04:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/1021644", "https://ndownloader.figshare.com/files/1021645", "https://ndownloader.figshare.com/files/1021646", "https://ndownloader.figshare.com/files/1021647", "https://ndownloader.figshare.com/files/1021648", "https://ndownloader.figshare.com/files/1021649", "https://ndownloader.figshare.com/files/1021650"], "description"=>"<div><p>Background</p><p>The marine epiphytic dinoflagellate genus <i>Gambierdiscus</i> produce toxins that cause ciguatera fish poisoning (CFP): one of the most significant seafood-borne illnesses associated with fish consumption worldwide. So far, occurrences of CFP incidents in Japan have been mainly reported in subtropical areas. A previous phylogeographic study of Japanese <i>Gambierdiscus</i> revealed the existence of two distinct phylotypes: <i>Gambierdiscus</i> sp. type 1 from subtropical and <i>Gambierdiscus</i> sp. type 2 from temperate areas. However, details of the genetic diversity and distribution for Japanese <i>Gambierdiscus</i> are still unclear, because a comprehensive investigation has not been conducted yet.</p><p>Methods/Principal Finding</p><p>A total of 248 strains were examined from samples mainly collected from western and southern coastal areas of Japan during 2006–2011. The SSU rDNA, the LSU rDNA D8–D10 and the ITS region were selected as genetic markers and phylogenetic analyses were conducted. The genetic diversity of Japanese <i>Gambierdiscus</i> was high since five species/phylotypes were detected: including two reported phylotypes (<i>Gambierdiscus</i> sp. type 1 and <i>Gambierdiscus</i> sp. type 2), two species of <i>Gambierdiscus</i> (<i>G. australes</i> and <i>G</i>. cf. <i>yasumotoi</i>) and a hitherto unreported phylotype <i>Gambierdiscus</i> sp. type 3. The distributions of type 3 and <i>G</i>. cf. <i>yasumotoi</i> were restricted to the temperate and the subtropical area, respectively. On the other hand, type 1, type 2 and <i>G. australes</i> occurred from the subtropical to the temperate area, with a tendency that type 1 and <i>G. australes</i> were dominant in the subtropical area, whereas type 2 was dominant in the temperate area. By using mouse bioassay, type 1, type 3 and <i>G. australes</i> exhibited mouse toxicities.</p><p>Conclusions/Significance</p><p>This study revealed a surprising diversity of Japanese <i>Gambierdiscus</i> and the distribution of five species/phylotypes displayed clear geographical patterns in Japanese coastal areas. The SSU rDNA and the LSU rDNA D8–D10 as genetic markers are recommended for further use.</p></div>", "links"=>[], "tags"=>["Computational biology", "population genetics", "Genetic drift", "Sequence analysis", "Evolutionary biology", "Evolutionary systematics", "phylogenetics", "Model organisms", "Animal models", "mouse", "marine and aquatic sciences", "toxicology", "Veterinary toxicology", "ciguatera-causing", "dinoflagellate", "areas", "japan"], "article_id"=>680269, "categories"=>["Medicine", "Biological Sciences", "Earth and Environmental Sciences"], "users"=>["Tomohiro Nishimura", "Shinya Sato", "Wittaya Tawong", "Hiroshi Sakanari", "Keita Uehara", "Md Mahfuzur Rahman Shah", "Shoichiro Suda", "Takeshi Yasumoto", "Yohsuke Taira", "Haruo Yamaguchi", "Masao Adachi"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0060882.s001", "https://dx.doi.org/10.1371/journal.pone.0060882.s002", "https://dx.doi.org/10.1371/journal.pone.0060882.s003", "https://dx.doi.org/10.1371/journal.pone.0060882.s004", "https://dx.doi.org/10.1371/journal.pone.0060882.s005", "https://dx.doi.org/10.1371/journal.pone.0060882.s006", "https://dx.doi.org/10.1371/journal.pone.0060882.s007"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Genetic_Diversity_and_Distribution_of_the_Ciguatera_Causing_Dinoflagellate_Gambierdiscus_spp_Dinophyceae_in_Coastal_Areas_of_Japan/680269", "title"=>"Genetic Diversity and Distribution of the Ciguatera-Causing Dinoflagellate <i>Gambierdiscus</i> spp. (Dinophyceae) in Coastal Areas of Japan", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-04-11 00:04:29"}

PMC Usage Stats

  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"35", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"27", "full-text"=>"26", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"28", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"20", "full-text"=>"39", "pdf"=>"9", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"28", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"15", "full-text"=>"20", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"23", "full-text"=>"26", "pdf"=>"3", "abstract"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"20", "full-text"=>"26", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"3", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"4", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"4", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"15", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"72", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"13", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"12", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"14", "full-text"=>"7", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"5", "cited-by"=>"1", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"1", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"18", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"7", "cited-by"=>"1", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"9"}

Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[269, 466, 588, 697, 800, 896, 988, 1076, 1165, 1254, 1340, 1417]}, {"subject_area"=>"/Biology and life sciences/Biogeography", "average_usage"=>[321, 494, 634, 734, 850, 962, 1060, 1140, 1226, 1319, 1409, 1493, 1559]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[284, 491, 620, 738, 843, 945, 1043, 1137, 1225, 1315, 1400, 1479, 1555]}, {"subject_area"=>"/Biology and life sciences/Organisms", "average_usage"=>[281, 484, 611, 728, 835, 934, 1030, 1123, 1214, 1299, 1383, 1464]}, {"subject_area"=>"/Biology and life sciences/Population biology", "average_usage"=>[269, 448, 558, 658, 744, 830, 914, 995, 1068, 1139, 1214, 1284, 1349]}, {"subject_area"=>"/Biology and life sciences/Zoology", "average_usage"=>[294, 473, 591, 693, 788, 883, 972, 1054, 1140, 1222, 1299, 1381, 1446]}, {"subject_area"=>"/Computer and information sciences", "average_usage"=>[297, 488, 616, 724, 828, 939, 1038, 1127, 1223, 1311, 1393, 1479, 1556]}, {"subject_area"=>"/Computer and information sciences/Data management", "average_usage"=>[301, 521, 654, 776, 898, 1028, 1133, 1258, 1373, 1457, 1542, 1648, 1738]}, {"subject_area"=>"/Earth sciences", "average_usage"=>[296, 488, 620, 717, 828, 938, 1038, 1130, 1230, 1328, 1414, 1502, 1592]}, {"subject_area"=>"/Ecology and environmental sciences/Biogeography", "average_usage"=>[320, 517, 642, 738, 850, 955, 1039, 1136, 1226, 1315, 1406, 1495, 1565]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[264, 460, 584, 692, 794, 887, 978, 1067, 1154, 1241, 1328, 1408, 1474]}, {"subject_area"=>"/People and places/Geographical locations", "average_usage"=>[263, 456, 571, 673, 776, 866, 955, 1040, 1132, 1217, 1310, 1391, 1454]}]}
Loading … Spinner
There are currently no alerts