In Vitro HIV-1 Evolution in Response to Triple Reverse Transcriptase Inhibitors & In Silico Phenotypic Analysis
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"In Vitro HIV-1 Evolution in Response to Triple Reverse Transcriptase Inhibitors & In Silico Phenotypic Analysis", "type"=>"journal", "authors"=>[{"first_name"=>"Barbara A.", "last_name"=>"Rath", "scopus_author_id"=>"23028714000"}, {"first_name"=>"Kaveh Pouran", "last_name"=>"Yousef", "scopus_author_id"=>"55945878900"}, {"first_name"=>"David K.", "last_name"=>"Katzenstein", "scopus_author_id"=>"35369920000"}, {"first_name"=>"Robert W.", "last_name"=>"Shafer", "scopus_author_id"=>"7102887874"}, {"first_name"=>"Christof", "last_name"=>"Schütte", "scopus_author_id"=>"7005968052"}, {"first_name"=>"Max", "last_name"=>"von Kleist", "scopus_author_id"=>"15046062000"}, {"first_name"=>"Thomas C.", "last_name"=>"Merigan", "scopus_author_id"=>"35395799000"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"23613794", "sgr"=>"84876206921", "scopus"=>"2-s2.0-84876206921", "doi"=>"10.1371/journal.pone.0061102", "pui"=>"368743285", "issn"=>"19326203"}, "id"=>"924a5e77-3c8a-306c-b4ce-66c61672d0d6", "abstract"=>"BACKGROUND: Effectiveness of ART regimens strongly depends upon complex interactions between the selective pressure of drugs and the evolution of mutations that allow or restrict drug resistance. METHODS: Four clinical isolates from NRTI-exposed, NNRTI-naive subjects were passaged in increasing concentrations of NVP in combination with 1 µM 3 TC and 2 µM ADV to assess selective pressures of multi-drug treatment. A novel parameter inference procedure, based on a stochastic viral growth model, was used to estimate phenotypic resistance and fitness from in vitro combination passage experiments. RESULTS: Newly developed mathematical methods estimated key phenotypic parameters of mutations arising through selective pressure exerted by 3 TC and NVP. Concentrations of 1 µM 3 TC maintained the M184V mutation, which was associated with intrinsic fitness deficits. Increasing NVP concentrations selected major NNRTI resistance mutations. The evolutionary pathway of NVP resistance was highly dependent on the viral genetic background, epistasis as well as stochasticity. Parameter estimation indicated that the previously unrecognized mutation L228Q was associated with NVP resistance in some isolates. CONCLUSION: Serial passage of viruses in the presence of multiple drugs may resemble the selection of mutations observed among treated individuals and populations in vivo and indicate evolutionary preferences and restrictions. Phenotypic resistance estimated here \"in silico\" from in vitro passage experiments agreed well with previous knowledge, suggesting that the unique combination of \"wet-\" and \"dry-lab\" experimentation may improve our understanding of HIV-1 resistance evolution in the future.", "link"=>"http://www.mendeley.com/research/vitro-hiv1-evolution-response-triple-reverse-transcriptase-inhibitors-silico-phenotypic-analysis", "reader_count"=>9, "reader_count_by_academic_status"=>{"Student > Doctoral Student"=>2, "Researcher"=>3, "Student > Ph. D. Student"=>1, "Student > Bachelor"=>1, "Professor"=>1, "Student > Master"=>1}, "reader_count_by_user_role"=>{"Student > Doctoral Student"=>2, "Researcher"=>3, "Student > Ph. D. Student"=>1, "Student > Bachelor"=>1, "Professor"=>1, "Student > Master"=>1}, "reader_count_by_subject_area"=>{"Agricultural and Biological Sciences"=>7, "Medicine and Dentistry"=>1, "Physics and Astronomy"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>7}}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84876206921"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84876206921?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84876206921&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84876206921&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84876206921", "dc:identifier"=>"SCOPUS_ID:84876206921", "eid"=>"2-s2.0-84876206921", "dc:title"=>"In Vitro HIV-1 Evolution in Response to Triple Reverse Transcriptase Inhibitors & In Silico Phenotypic Analysis", "dc:creator"=>"Rath B.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"8", "prism:issueIdentifier"=>"4", "prism:pageRange"=>nil, "prism:coverDate"=>"2013-04-17", "prism:coverDisplayDate"=>"17 April 2013", "prism:doi"=>"10.1371/journal.pone.0061102", "citedby-count"=>"6", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Charité – Universitätsmedizin Berlin", "affiliation-city"=>"Berlin", "affiliation-country"=>"Germany"}, {"@_fa"=>"true", "affilname"=>"Stanford University Medical Center", "affiliation-city"=>"Stanford", "affiliation-country"=>"United States"}], "pubmed-id"=>"23613794", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e61102", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0061102", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"35", "xml_views"=>"5", "html_views"=>"240"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"24", "xml_views"=>"4", "html_views"=>"120"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"37"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"54"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"50"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"52"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"56"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"20"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"38"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"15"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"3", "html_views"=>"4"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"0"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"5"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"4"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"8", "year"=>"2020", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"1"}
  • {"month"=>"9", "year"=>"2020", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"10", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"11", "year"=>"2020", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"12", "year"=>"2020", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"0"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1026542"], "description"=>"<p>The solid red horizontal lines indicate the respective median passage times, whereas the boxes surrounding them indicate the range encompassed by the 25<sup>th</sup> and 75<sup>th</sup> percentiles. The whiskers denote the most extreme data points, which are not considered outliers and the black dots indicate outliers. A: Viral passage times for isolate #1. B: Viral passage times for isolate #2. C: Viral passage times for isolate #4. D: Viral passage times for isolate #5.</p>", "links"=>[], "tags"=>["microbiology", "Virology", "Viral replication", "Viral nucleic acid", "antivirals", "Immunodeficiency viruses", "Mechanisms of resistance and susceptibility", "Viral evolution", "Virulence factors and mechanisms", "Probability theory", "Markov model", "Stochastic processes", "Infectious diseases", "Viral diseases", "hiv", "HIV epidemiology", "Retrovirology and HIV immunopathogenesis", "Infectious disease modeling", "times", "set-ups", "drugs", "tc", "adv", "indicated"], "article_id"=>684096, "categories"=>["Mathematics", "Medicine", "Biological Sciences"], "users"=>["Barbara A. Rath", "Kaveh Pouran Yousef", "David K. Katzenstein", "Robert W. Shafer", "Christof Schütte", "Max von Kleist", "Thomas C. Merigan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061102.g004", "stats"=>{"downloads"=>1, "page_views"=>21, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Box_plot_of_passage_times_for_virus_isolate_1_2_4_amp_5_during_experimental_set_ups_A_amp_B_no_drugs_added_vs_1_181_M_3_TC_plus_2_181_M_ADV_added_as_indicated_on_the_x_axis_/684096", "title"=>"Box plot of passage times for virus isolate #1, #2, #4 & #5 during experimental set-ups A & B (no drugs added vs. 1 µM 3 TC plus 2 µM ADV added) as indicated on the x-axis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-17 01:08:16"}
  • {"files"=>["https://ndownloader.figshare.com/files/1026548", "https://ndownloader.figshare.com/files/1026549", "https://ndownloader.figshare.com/files/1026551"], "description"=>"<div><p>Background</p><p>Effectiveness of ART regimens strongly depends upon complex interactions between the selective pressure of drugs and the evolution of mutations that allow or restrict drug resistance.</p><p>Methods</p><p>Four clinical isolates from NRTI-exposed, NNRTI-naive subjects were passaged in increasing concentrations of NVP in combination with 1 µM 3 TC and 2 µM ADV to assess selective pressures of multi-drug treatment. A novel parameter inference procedure, based on a stochastic viral growth model, was used to estimate phenotypic resistance and fitness from <i>in vitro</i> combination passage experiments.</p><p>Results</p><p>Newly developed mathematical methods estimated key phenotypic parameters of mutations arising through selective pressure exerted by 3 TC and NVP. Concentrations of 1 µM 3 TC maintained the M184V mutation, which was associated with intrinsic fitness deficits. Increasing NVP concentrations selected major NNRTI resistance mutations. The evolutionary pathway of NVP resistance was highly dependent on the viral genetic background, epistasis as well as stochasticity. Parameter estimation indicated that the previously unrecognized mutation L228Q was associated with NVP resistance in some isolates.</p><p>Conclusion</p><p>Serial passage of viruses in the presence of multiple drugs may resemble the selection of mutations observed among treated individuals and populations <i>in vivo</i> and indicate evolutionary preferences and restrictions. Phenotypic resistance estimated here “<i>in silico</i>” from <i>in vitro</i> passage experiments agreed well with previous knowledge, suggesting that the unique combination of “wet-” and “dry-lab” experimentation may improve our understanding of HIV-1 resistance evolution in the future.</p></div>", "links"=>[], "tags"=>["microbiology", "Virology", "Viral replication", "Viral nucleic acid", "antivirals", "Immunodeficiency viruses", "Mechanisms of resistance and susceptibility", "Viral evolution", "Virulence factors and mechanisms", "Probability theory", "Markov model", "Stochastic processes", "Infectious diseases", "Viral diseases", "hiv", "HIV epidemiology", "Retrovirology and HIV immunopathogenesis", "Infectious disease modeling", "hiv-1", "triple", "transcriptase", "inhibitors", "phenotypic"], "article_id"=>684102, "categories"=>["Mathematics", "Medicine", "Biological Sciences"], "users"=>["Barbara A. Rath", "Kaveh Pouran Yousef", "David K. Katzenstein", "Robert W. Shafer", "Christof Schütte", "Max von Kleist", "Thomas C. Merigan"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0061102.s001", "https://dx.doi.org/10.1371/journal.pone.0061102.s002", "https://dx.doi.org/10.1371/journal.pone.0061102.s003"], "stats"=>{"downloads"=>17, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_In_Vitro_HIV_1_Evolution_in_Response_to_Triple_Reverse_Transcriptase_Inhibitors_In_Silico_Phenotypic_Analysis/684102", "title"=>"<i>In Vitro</i> HIV-1 Evolution in Response to Triple Reverse Transcriptase Inhibitors & <i>In Silico</i> Phenotypic Analysis", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-04-17 01:08:22"}
  • {"files"=>["https://ndownloader.figshare.com/files/1026545"], "description"=>"<p><i>r</i><sub>0/</sub> (baseline growth rate in the absence of drugs), intensity- <i>η</i><sub>NRTI</sub> and probability <i>ρ</i><sub>NRTI</sub> of NRTI-induced effect at concentrations of 1 µM 3 TC and 2 µM ADV respectively and lower-bound estimates for the susceptibility of baseline isolates towards NVP IC<sub>50</sub>. Indicated numbers are median estimates from the <i>k-</i>best models (see Mathematical Methods) and their respective 5<sup>th</sup>–and 95<sup>th</sup> –percentiles (in brackets).</p>", "links"=>[], "tags"=>["microbiology", "Virology", "Viral replication", "Viral nucleic acid", "antivirals", "Immunodeficiency viruses", "Mechanisms of resistance and susceptibility", "Viral evolution", "Virulence factors and mechanisms", "Probability theory", "Markov model", "Stochastic processes", "Infectious diseases", "Viral diseases", "hiv", "HIV epidemiology", "Retrovirology and HIV immunopathogenesis", "Infectious disease modeling", "baseline"], "article_id"=>684099, "categories"=>["Mathematics", "Medicine", "Biological Sciences"], "users"=>["Barbara A. Rath", "Kaveh Pouran Yousef", "David K. Katzenstein", "Robert W. Shafer", "Christof Schütte", "Max von Kleist", "Thomas C. Merigan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061102.t002", "stats"=>{"downloads"=>2, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Estimated_baseline_parameters_/684099", "title"=>"Estimated baseline parameters.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-04-17 01:08:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1026544"], "description"=>"<p>A small value (close to 0) denotes a large fitness loss, whereas a value close to 1 denotes no fitness deficit. Values indicated are medians of all parameter estimates. The 5<sup>th</sup> and 95<sup>th</sup> percentile of estimates are indicated in brackets. 'n.ds’ means ‘not deselected’ and n.i. means parameter ‘not identifiable’. Parameters <i>f</i>(67S), <i>f</i>(208H), <i>f</i>(35I) and <i>f</i>(210W/211K) could not be reliably estimated from the data or were not significantly different from the value 1.</p>", "links"=>[], "tags"=>["microbiology", "Virology", "Viral replication", "Viral nucleic acid", "antivirals", "Immunodeficiency viruses", "Mechanisms of resistance and susceptibility", "Viral evolution", "Virulence factors and mechanisms", "Probability theory", "Markov model", "Stochastic processes", "Infectious diseases", "Viral diseases", "hiv", "HIV epidemiology", "Retrovirology and HIV immunopathogenesis", "Infectious disease modeling", "mutations", "baseline", "isolates"], "article_id"=>684098, "categories"=>["Mathematics", "Medicine", "Biological Sciences"], "users"=>["Barbara A. Rath", "Kaveh Pouran Yousef", "David K. Katzenstein", "Robert W. Shafer", "Christof Schütte", "Max von Kleist", "Thomas C. Merigan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061102.t004", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Estimated_relative_fitness_deficit_f_q_of_mutations_present_in_the_genetic_background_of_baseline_isolates_1_2_3_4_5_/684098", "title"=>"Estimated relative fitness deficit <i>f(q)</i> of mutations present in the genetic background of baseline isolates #1,#2/3,#4,#5.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-04-17 01:08:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/1026539"], "description"=>"<p>The solid red horizontal lines indicate the respective median passage times, whereas the boxes surrounding them indicate the range encompassed by the 25<sup>th</sup> and 75<sup>th</sup> percentiles. The whiskers denote the most extreme data points, which are not considered outliers and the black dots indicate outliers. A: Viral passage times for isolate #1. B: Viral passage times for isolate #2&3 (combined). C: Viral passage times for isolate #4. D: Viral passage times for isolate #5.</p>", "links"=>[], "tags"=>["microbiology", "Virology", "Viral replication", "Viral nucleic acid", "antivirals", "Immunodeficiency viruses", "Mechanisms of resistance and susceptibility", "Viral evolution", "Virulence factors and mechanisms", "Probability theory", "Markov model", "Stochastic processes", "Infectious diseases", "Viral diseases", "hiv", "HIV epidemiology", "Retrovirology and HIV immunopathogenesis", "Infectious disease modeling", "times", "set-ups", "indicated"], "article_id"=>684093, "categories"=>["Mathematics", "Medicine", "Biological Sciences"], "users"=>["Barbara A. Rath", "Kaveh Pouran Yousef", "David K. Katzenstein", "Robert W. Shafer", "Christof Schütte", "Max von Kleist", "Thomas C. Merigan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061102.g002", "stats"=>{"downloads"=>0, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Box_plot_of_single_passage_times_for_virus_isolate_1_2_3_4_amp_5_during_experimental_set_ups_A_8211_F_as_indicated_on_the_x_axis_/684093", "title"=>"Box plot of single passage times for virus isolate #1, #2/3, #4 & #5 during experimental set-ups A–F as indicated on the x-axis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-17 01:08:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1026538"], "description"=>"<p>The illustration provides a complete review of RT sequence changes under the following experimental set-ups: A: no drugs were added to the media, B: [1 µM] 3 TC and [2 µM] ADV were added and maintained, C: NVP was added and concentrations were doubled for each passage (0.01 µM NVP during the first passage up to 20.48 µM during the last passage), D: [2 µM] ADV and increasing concentrations of NVP were added, E: [1 µM] 3 TC and increasing concentrations of NVP were added and F: [1 µM] 3 TC and [2 µM] ADV and increasing concentrations of NVP were added to the medium. Individual isolates #1 to #5 are indicated above the columns. Sequence changes listed are indicated in the rows that correspond to the passage number where they were first observed. NVP concentrations used in the respective passage experiment are listed on the right in units µM. Any mutation away from wild-type (Hxb2 strain) is indicated by a rightward-pointing arrow, whereas reversal to wild-type is indicated by a left-ward pointing arrow. All sequence changes (novel mutations and reversals) persisted throughout passage 12.</p>", "links"=>[], "tags"=>["microbiology", "Virology", "Viral replication", "Viral nucleic acid", "antivirals", "Immunodeficiency viruses", "Mechanisms of resistance and susceptibility", "Viral evolution", "Virulence factors and mechanisms", "Probability theory", "Markov model", "Stochastic processes", "Infectious diseases", "Viral diseases", "hiv", "HIV epidemiology", "Retrovirology and HIV immunopathogenesis", "Infectious disease modeling", "experiments", "sequencing"], "article_id"=>684092, "categories"=>["Mathematics", "Medicine", "Biological Sciences"], "users"=>["Barbara A. Rath", "Kaveh Pouran Yousef", "David K. Katzenstein", "Robert W. Shafer", "Christof Schütte", "Max von Kleist", "Thomas C. Merigan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061102.g001", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Summary_of_passage_experiments_with_sequencing_data_/684092", "title"=>"Summary of passage experiments with sequencing data.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-17 01:08:12"}
  • {"files"=>["https://ndownloader.figshare.com/files/1026547"], "description"=>"<p>Baseline amino acid substitutions in relation to reference sequence (Hxb2) from the Stanford HIVDB <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061102#pone.0061102-Rhee1\" target=\"_blank\">[28]</a>.</p>", "links"=>[], "tags"=>["microbiology", "Virology", "Viral replication", "Viral nucleic acid", "antivirals", "Immunodeficiency viruses", "Mechanisms of resistance and susceptibility", "Viral evolution", "Virulence factors and mechanisms", "Probability theory", "Markov model", "Stochastic processes", "Infectious diseases", "Viral diseases", "hiv", "HIV epidemiology", "Retrovirology and HIV immunopathogenesis", "Infectious disease modeling", "amino", "substitutions", "stanford", "hivdb"], "article_id"=>684101, "categories"=>["Mathematics", "Medicine", "Biological Sciences"], "users"=>["Barbara A. Rath", "Kaveh Pouran Yousef", "David K. Katzenstein", "Robert W. Shafer", "Christof Schütte", "Max von Kleist", "Thomas C. Merigan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061102.t001", "stats"=>{"downloads"=>2, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Baseline_amino_acid_substitutions_in_relation_to_reference_sequence_Hxb2_from_the_Stanford_HIVDB_28_/684101", "title"=>"Baseline amino acid substitutions in relation to reference sequence (Hxb2) from the Stanford HIVDB [28].", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-04-17 01:08:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/1026546"], "description"=>"<p>Values indicated are medians of all parameter estimates and the 5<sup>th</sup> and 95<sup>th</sup> percentile of the estimates are indicated in brackets. 'n.s’ means ‘not selected’ and n.i. means parameter ‘not identifiable’. Parameters FR(69I), FR(103N), FR(122K), FR(128Q), FR(208Y) and FR(218G) were not identifiable from the data for any isolates and thus omitted from the table.</p>", "links"=>[], "tags"=>["microbiology", "Virology", "Viral replication", "Viral nucleic acid", "antivirals", "Immunodeficiency viruses", "Mechanisms of resistance and susceptibility", "Viral evolution", "Virulence factors and mechanisms", "Probability theory", "Markov model", "Stochastic processes", "Infectious diseases", "Viral diseases", "hiv", "HIV epidemiology", "Retrovirology and HIV immunopathogenesis", "Infectious disease modeling", "lower-bounds", "nvp", "exerted", "amino", "substitutions", "baseline"], "article_id"=>684100, "categories"=>["Mathematics", "Medicine", "Biological Sciences"], "users"=>["Barbara A. Rath", "Kaveh Pouran Yousef", "David K. Katzenstein", "Robert W. Shafer", "Christof Schütte", "Max von Kleist", "Thomas C. Merigan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061102.t003", "stats"=>{"downloads"=>4, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Estimated_lower_bounds_of_fold_resistance_against_NVP_exerted_by_single_amino_acid_substitutions_in_the_distinct_genetic_background_of_the_baseline_isolates_/684100", "title"=>"Estimated lower-bounds of fold resistance against NVP exerted by single amino acid substitutions in the distinct genetic background of the baseline isolates.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-04-17 01:08:20"}
  • {"files"=>["https://ndownloader.figshare.com/files/1026541"], "description"=>"<p>A: Average number of mutations per passage in experiments with NVP (experimental set-ups C, D, E & F). Asterisks indicate whether there were significantly more mutations (Wilcoxon rank sum test) than in the NVP-free experiments (experimental set-ups A & B). *p<0.1, **p<0.05, ***p<0.01. B: Cumulative probability of detecting no mutation. The blue and red lines show the cumulative probability of not detecting a mutation after the indicated numbers of passages (x-axis) in experiments where NVP was added with increasing concentrations (blue line; experimental set-ups C, D, E & F) vs. experiments where no NVP was added (red line; experimental set-ups A & B).</p>", "links"=>[], "tags"=>["microbiology", "Virology", "Viral replication", "Viral nucleic acid", "antivirals", "Immunodeficiency viruses", "Mechanisms of resistance and susceptibility", "Viral evolution", "Virulence factors and mechanisms", "Probability theory", "Markov model", "Stochastic processes", "Infectious diseases", "Viral diseases", "hiv", "HIV epidemiology", "Retrovirology and HIV immunopathogenesis", "Infectious disease modeling"], "article_id"=>684095, "categories"=>["Mathematics", "Medicine", "Biological Sciences"], "users"=>["Barbara A. Rath", "Kaveh Pouran Yousef", "David K. Katzenstein", "Robert W. Shafer", "Christof Schütte", "Max von Kleist", "Thomas C. Merigan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061102.g003", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Selection_dynamics_/684095", "title"=>"Selection dynamics.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-17 01:08:15"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"34", "full-text"=>"34", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"13", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"0", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"2", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"3", "pdf"=>"0", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"0", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"17", "full-text"=>"18", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"18", "full-text"=>"16", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"10"}

Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[266, 468, 593, 703, 804, 903, 993, 1084, 1171, 1256, 1339, 1422, 1492]}, {"subject_area"=>"/Biology and life sciences/Evolutionary biology", "average_usage"=>[302, 488, 607, 717, 832, 931, 1024, 1117, 1212, 1302, 1389, 1469, 1535]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[284, 491, 620, 738, 843, 945, 1043, 1137, 1225, 1315, 1400, 1479, 1555]}, {"subject_area"=>"/Biology and life sciences/Microbiology", "average_usage"=>[293, 503, 638, 755, 861, 960, 1056, 1146, 1239, 1323, 1403, 1491, 1568]}, {"subject_area"=>"/Medicine and health sciences/Pharmaceutics", "average_usage"=>[254, 457, 591, 700, 808, 906, 1001, 1093, 1185, 1276, 1373, 1457, 1535]}, {"subject_area"=>"/Medicine and health sciences/Pharmacology", "average_usage"=>[265, 471, 604, 723, 827, 926, 1022, 1113, 1210, 1290, 1377, 1454, 1532]}]}
Loading … Spinner
There are currently no alerts