Mechanisms Underlying the Anti-Tumoral Effects of Citrus bergamia Juice
Publication Date
April 16, 2013
Journal
PLOS ONE
Authors
Simona Delle Monache, Patrizia Sanità, Elena Trapasso, Maria Rita Ursino, et al
Volume
8
Issue
4
Pages
e61484
DOI
https://dx.plos.org/10.1371/journal.pone.0061484
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0061484
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/23613861
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3628853
Europe PMC
http://europepmc.org/abstract/MED/23613861
Web of Science
000317893400068
Scopus
84876183786
Mendeley
http://www.mendeley.com/research/mechanisms-underlying-antitumoral-effects-citrus-bergamia-juice
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Mendeley | Further Information

{"title"=>"Mechanisms Underlying the Anti-Tumoral Effects of Citrus bergamia Juice", "type"=>"journal", "authors"=>[{"first_name"=>"Simona", "last_name"=>"Delle Monache", "scopus_author_id"=>"6506190216"}, {"first_name"=>"Patrizia", "last_name"=>"Sanità", "scopus_author_id"=>"37112966100"}, {"first_name"=>"Elena", "last_name"=>"Trapasso", "scopus_author_id"=>"23111601800"}, {"first_name"=>"Maria Rita", "last_name"=>"Ursino", "scopus_author_id"=>"35887827700"}, {"first_name"=>"Paola", "last_name"=>"Dugo", "scopus_author_id"=>"7003836481"}, {"first_name"=>"Marina", "last_name"=>"Russo", "scopus_author_id"=>"56186339200"}, {"first_name"=>"Nadia", "last_name"=>"Ferlazzo", "scopus_author_id"=>"24474430500"}, {"first_name"=>"Gioacchino", "last_name"=>"Calapai", "scopus_author_id"=>"7004375991"}, {"first_name"=>"Adriano", "last_name"=>"Angelucci", "scopus_author_id"=>"7003625804"}, {"first_name"=>"Michele", "last_name"=>"Navarra", "scopus_author_id"=>"55618434400"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"23613861", "sgr"=>"84876183786", "doi"=>"10.1371/journal.pone.0061484", "scopus"=>"2-s2.0-84876183786", "pui"=>"368726712", "issn"=>"19326203"}, "id"=>"1426f5a1-00e7-3843-a513-4a27a5166cb7", "abstract"=>"Based on the growing deal of data concerning the biological activity of flavonoid-rich natural products, the aim of the present study was to explore in vitro the potential anti-tumoral activity of Citrus Bergamia (bergamot) juice (BJ), determining its molecular interaction with cancer cells. Here we show that BJ reduced growth rate of different cancer cell lines, with the maximal growth inhibition observed in neuroblastoma cells (SH-SY5Y) after 72 hs of exposure to 5% BJ. The SH-SY5Y antiproliferative effect elicited by BJ was not due to a cytotoxic action and it did not induce apoptosis. Instead, BJ stimulated the arrest in the G1 phase of cell cycle and determined a modification in cellular morphology, causing a marked increase of detached cells. The inhibition of adhesive capacity on different physiologic substrates and on endothelial cells monolayer were correlated with an impairment of actin filaments, a reduction in the expression of the active form of focal adhesion kinase (FAK) that in turn caused inhibition of cell migration. In parallel, BJ seemed to hinder the association between the neural cell adhesion molecule (NCAM) and FAK. Our data suggest a mechanisms through which BJ can inhibit important molecular pathways related to cancer-associated aggressive phenotype and offer new suggestions for further studies on the role of BJ in cancer treatment.", "link"=>"http://www.mendeley.com/research/mechanisms-underlying-antitumoral-effects-citrus-bergamia-juice", "reader_count"=>16, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Librarian"=>2, "Researcher"=>5, "Student > Ph. D. Student"=>1, "Student > Master"=>4, "Other"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Librarian"=>2, "Researcher"=>5, "Student > Ph. D. Student"=>1, "Student > Master"=>4, "Other"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Unspecified"=>2, "Agricultural and Biological Sciences"=>5, "Medicine and Dentistry"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Chemistry"=>2, "Computer Science"=>1, "Immunology and Microbiology"=>1, "Biochemistry, Genetics and Molecular Biology"=>2}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>5}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>2}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"United States"=>1}, "group_count"=>4}

CrossRef

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1025423"], "description"=>"<p>Results show that BJ arrests cell cycle at G1 phase, reducing S and G2/M phases in time and concentration dependent manner. Data are represented as percentages of the total cell population and are the mean ± SEM of five independent experiments.</p>", "links"=>[], "tags"=>["Molecular cell biology", "cell adhesion", "ncam", "Cell growth", "Signal transduction", "Drugs and devices", "Pharmacodynamics", "oncology", "Basic cancer research", "metastasis", "Cancer treatment", "Complementary and alternative medicine", "Cancers and neoplasms", "Neurological tumors", "Oncology agents"], "article_id"=>683198, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Simona Delle Monache", "Patrizia Sanità", "Elena Trapasso", "Maria Rita Ursino", "Paola Dugo", "Marina Russo", "Nadia Ferlazzo", "Gioacchino Calapai", "Adriano Angelucci", "Michele Navarra"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061484.t002", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_BJ_effect_on_cell_cycle_progression_/683198", "title"=>"BJ effect on cell cycle progression.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-04-16 00:53:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/1025419"], "description"=>"<p>Western blotting analysis of FAK and pFAK expression in SH-SY5Y and PC12 cells treated for 24 hs with increasing concentration of BJ (0.1%, 0.5% and 1%). Relative densitometric analyses of pFAK and FAK immunoreactive bands are presented in the histograms. Data (mean ± SEM of three experiments) were normalized to the values yielded for β-actin. *P<0.05, **P<0.01 and ***P<0.001 <i>vs</i> untreated cells. Note the significant reduction of pFAK by BJ.</p>", "links"=>[], "tags"=>["Molecular cell biology", "cell adhesion", "ncam", "Cell growth", "Signal transduction", "Drugs and devices", "Pharmacodynamics", "oncology", "Basic cancer research", "metastasis", "Cancer treatment", "Complementary and alternative medicine", "Cancers and neoplasms", "Neurological tumors", "Oncology agents", "pfak", "sh-sy5y", "pc12", "cells", "treated"], "article_id"=>683194, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Simona Delle Monache", "Patrizia Sanità", "Elena Trapasso", "Maria Rita Ursino", "Paola Dugo", "Marina Russo", "Nadia Ferlazzo", "Gioacchino Calapai", "Adriano Angelucci", "Michele Navarra"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061484.g008", "stats"=>{"downloads"=>4, "page_views"=>37, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_FAK_and_pFAK_expression_in_SH_SY5Y_and_PC12_cells_treated_with_BJ_/683194", "title"=>"FAK and pFAK expression in SH-SY5Y and PC12 cells treated with BJ.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-16 00:53:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/1025413"], "description"=>"<p>On the left of the panel are display the immunoblot of SH-SY5Y cells exposed to 1–5% BJ for 24–72 hs: a representative of three separate experiments is shown. On the right of the panel are presented the quantification ± SEM of both cyclin D1 and p53 expressions from three independent blots performed after 24–72 hs of BJ incubation. Autoradiographic bands were quantified by ImageJ software and normalized for β-actin levels. Data are extrapolated in reference of the values detected in control cells, which are arbitrarily assigned as 1. Results of Western blot documented that BJ decreases the expression of cyclin D1, unchanging the level of p53. *P<0.05 <i>vs</i> ctrl.</p>", "links"=>[], "tags"=>["Molecular cell biology", "cell adhesion", "ncam", "Cell growth", "Signal transduction", "Drugs and devices", "Pharmacodynamics", "oncology", "Basic cancer research", "metastasis", "Cancer treatment", "Complementary and alternative medicine", "Cancers and neoplasms", "Neurological tumors", "Oncology agents", "p53", "cyclin", "d1", "sh-sy5y", "treated"], "article_id"=>683188, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Simona Delle Monache", "Patrizia Sanità", "Elena Trapasso", "Maria Rita Ursino", "Paola Dugo", "Marina Russo", "Nadia Ferlazzo", "Gioacchino Calapai", "Adriano Angelucci", "Michele Navarra"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061484.g005", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Analysis_of_p53_and_cyclin_D1_expression_in_SH_SY5Y_treated_with_BJ_/683188", "title"=>"Analysis of p53 and cyclin D1 expression in SH-SY5Y treated with BJ.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-16 00:53:08"}
  • {"files"=>["https://ndownloader.figshare.com/files/1025410"], "description"=>"<p>(A) Cytotoxic action of increasing concentrations of BJ (1–5%) was determined in SH-SY5Y, PC12 and WI-38 cells by trypan blue dye exclusion test. The assays were performed for 24, 48 and 72 hs and expressed as % of cell death. Data are the mean ± SEM of three independent experiments. Results display that BJ did not induced cytotoxicity neither in normal nor in tumoral cells. (B) Assessment of DNA damage in SH-SY5Y cells exposed to BJ performed by comet assay. In the panel are reported the images captured by fluorescence microscopy after 24 (on the left), 48 (in the middle) and 72 hs (on the right) of treatment. A representative experiment that was replicated three times with similar results is shown. The images display the round and intact nucleus observed in both BJ-treated and untreated cells, suggesting the lack of genotoxicity by BJ. HDNA: % head DNA. Nuclei were visualized by fluorescence microscopy at a magnification of 400x.</p>", "links"=>[], "tags"=>["Molecular cell biology", "cell adhesion", "ncam", "Cell growth", "Signal transduction", "Drugs and devices", "Pharmacodynamics", "oncology", "Basic cancer research", "metastasis", "Cancer treatment", "Complementary and alternative medicine", "Cancers and neoplasms", "Neurological tumors", "Oncology agents"], "article_id"=>683185, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Simona Delle Monache", "Patrizia Sanità", "Elena Trapasso", "Maria Rita Ursino", "Paola Dugo", "Marina Russo", "Nadia Ferlazzo", "Gioacchino Calapai", "Adriano Angelucci", "Michele Navarra"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061484.g003", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cytotoxic_effect_of_BJ_/683185", "title"=>"Cytotoxic effect of BJ.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-16 00:53:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1025409"], "description"=>"<p>PC-12 (A), MDA-MB231 (B), PC3 (C), WI-38 (D) and SH-SY5Y (E) cells were incubated with bergamot juice (from 0.5 to 5%) for 24, 48 and 72 hs and assayed by MTT test. Results are expressed as percentage of absorbance respect to control cells (100%). Analysis of the SH-SY5Y proliferation was performed also by cell count assays (F). Experimental data showed that, although with different extent, BJ reduced growth rate of several cell lines, with the maximal effect in the SH-SY5Y. The results are expressed as means ± SEM from at least three independent experiments performed in eightplicate (MTT test) or in triplicate (cell counting). *P<0.05 <i>vs</i> ctrl; **P<0.01 <i>vs</i> ctrl; ***P<0.001 <i>vs</i> ctrl, BJ 0.5 and 1%; °P<0.05 <i>vs</i> BJ 2.5%; <sup>∧</sup>P<0.05 <i>vs</i> BJ 1%.</p>", "links"=>[], "tags"=>["Molecular cell biology", "cell adhesion", "ncam", "Cell growth", "Signal transduction", "Drugs and devices", "Pharmacodynamics", "oncology", "Basic cancer research", "metastasis", "Cancer treatment", "Complementary and alternative medicine", "Cancers and neoplasms", "Neurological tumors", "Oncology agents", "bj"], "article_id"=>683184, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Simona Delle Monache", "Patrizia Sanità", "Elena Trapasso", "Maria Rita Ursino", "Paola Dugo", "Marina Russo", "Nadia Ferlazzo", "Gioacchino Calapai", "Adriano Angelucci", "Michele Navarra"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061484.g002", "stats"=>{"downloads"=>3, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_BJ_on_cell_proliferation_/683184", "title"=>"Effect of BJ on cell proliferation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-16 00:53:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/1025407"], "description"=>"<p>The analysis was performed at two different wavelengths, 283 (A) and 325 (B) nm. The sample was examined for five times and a representative chromatogram is shown. For peak identification see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061484#pone-0061484-t001\" target=\"_blank\">Table 1</a>.</p>", "links"=>[], "tags"=>["Molecular cell biology", "cell adhesion", "ncam", "Cell growth", "Signal transduction", "Drugs and devices", "Pharmacodynamics", "oncology", "Basic cancer research", "metastasis", "Cancer treatment", "Complementary and alternative medicine", "Cancers and neoplasms", "Neurological tumors", "Oncology agents", "chromatograms"], "article_id"=>683182, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Simona Delle Monache", "Patrizia Sanità", "Elena Trapasso", "Maria Rita Ursino", "Paola Dugo", "Marina Russo", "Nadia Ferlazzo", "Gioacchino Calapai", "Adriano Angelucci", "Michele Navarra"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061484.g001", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_RP_HPLC_chromatograms_of_BJ_/683182", "title"=>"RP-HPLC chromatograms of BJ.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-16 00:53:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/1025421"], "description"=>"<p>(A) Western blotting analysis of NCAM basal expression in PC3, MDA-MB231, WI-38, PC-12 and SH-SY5Y cells. Note that NCAM is expressed only in both SH-SY5Y and PC12 cells. (B) Western blotting analysis of FAK, pFAK, and NCAM expression in SH-SY5Y cells treated with 1% BJ for different times (from 1 h to 24 hs). Relative densitometric analyses of immunoreactive bands normalized to the values for β-actin are presented in the lateral histogram (mean ± SEM of three experiments). *P<0.05, **P<0.01 and ***P<0.001 <i>vs</i> untreated cells. A slight reduction of NCAM within 6 hs of BJ incubation accompanied to a significant decrease of pFAK is shown. (C) Protein extracts from SH-SY5Y cells treated for 24 hs with 0.1–5% BJ were subjected to immunoprecipitation with FAK antibody (IP:FAK, left panel), or with NCAM antibody (IP:NCAM; right panel) and then analysed for the expression of both NCAM and FAK.</p>", "links"=>[], "tags"=>["Molecular cell biology", "cell adhesion", "ncam", "Cell growth", "Signal transduction", "Drugs and devices", "Pharmacodynamics", "oncology", "Basic cancer research", "metastasis", "Cancer treatment", "Complementary and alternative medicine", "Cancers and neoplasms", "Neurological tumors", "Oncology agents", "fak"], "article_id"=>683196, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Simona Delle Monache", "Patrizia Sanità", "Elena Trapasso", "Maria Rita Ursino", "Paola Dugo", "Marina Russo", "Nadia Ferlazzo", "Gioacchino Calapai", "Adriano Angelucci", "Michele Navarra"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061484.g009", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_BJ_effects_on_association_between_FAK_and_NCAM_/683196", "title"=>"BJ effects on association between FAK and NCAM.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-16 00:53:16"}
  • {"files"=>["https://ndownloader.figshare.com/files/1025417"], "description"=>"<p>(A) Effect of BJ on SH-SY5Y migration through gelatine coated filters after 24 hs of treatment. In the upper panel representative images of coated-filters are shown. The graphic in the lower ones represents the mean ± SEM of the migrated cells, as described in the material and methods. **P<0.01 and ***P<0.001 <i>vs</i> control. (B) Fluorescence F-actin labelling of SH-SY5Y cells treated with 1% BJ. Representatives images show the loss of the normal actin morphology characterized by stress fibres (arrows) <i>vs</i> a morphology characterized by shorter and contorted actin fibres. White bar = 20 µm.</p>", "links"=>[], "tags"=>["Molecular cell biology", "cell adhesion", "ncam", "Cell growth", "Signal transduction", "Drugs and devices", "Pharmacodynamics", "oncology", "Basic cancer research", "metastasis", "Cancer treatment", "Complementary and alternative medicine", "Cancers and neoplasms", "Neurological tumors", "Oncology agents", "impairs", "sh-sy5y", "interfering", "actin"], "article_id"=>683192, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Simona Delle Monache", "Patrizia Sanità", "Elena Trapasso", "Maria Rita Ursino", "Paola Dugo", "Marina Russo", "Nadia Ferlazzo", "Gioacchino Calapai", "Adriano Angelucci", "Michele Navarra"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061484.g007", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_BJ_impairs_SH_SY5Y_migration_interfering_with_actin_polymerization_/683192", "title"=>"BJ impairs SH-SY5Y migration interfering with actin polymerization.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-16 00:53:12"}
  • {"files"=>["https://ndownloader.figshare.com/files/1025415"], "description"=>"<p>(A) Representative images showing the decreased adhesive capacity of SH-SY5Y cells following 2 hs of BJ treatment. Cells were visualized by optical microscopy at a magnification of 200x. (B) Adhesion assay performed by plating SH-SY5Y and PC12 cells on non-coated plastic surface for 24 hs. Data were expressed as percentage of the total cells present in the well and are the mean ± SEM of three different experiments. (C) Number of adhered cells obtained after plating SH-SY5Y cells on three different physiological substrates (Collagen I, Fibronectin and Matrigel) in presence of BJ for 24 hs. The values are expressed as mean percentage with respect to untreated cultures of three different experiments (± SEM). (D) Adhesive skill of SH-SY5Y cells treated with BJ at 0.5 and 1% in comparison with untreated cells when seeded on HUVEC monolayers. Representative images show the decrease capability of SH-SY5Y cell (see the number of round cells) to join to HUVEC cells. Values obtained by counting attached SH-SY5Y cells are shown in the histogram. Data are expressed as mean ± SEM counts of five different optical fields. *P<0.05; **P<0.01; ***P<0.001 <i>vs</i> control. Collectively the results demonstrated that BJ impaired cells adhesiveness.</p>", "links"=>[], "tags"=>["Molecular cell biology", "cell adhesion", "ncam", "Cell growth", "Signal transduction", "Drugs and devices", "Pharmacodynamics", "oncology", "Basic cancer research", "metastasis", "Cancer treatment", "Complementary and alternative medicine", "Cancers and neoplasms", "Neurological tumors", "Oncology agents", "inhibition", "bj"], "article_id"=>683190, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Simona Delle Monache", "Patrizia Sanità", "Elena Trapasso", "Maria Rita Ursino", "Paola Dugo", "Marina Russo", "Nadia Ferlazzo", "Gioacchino Calapai", "Adriano Angelucci", "Michele Navarra"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061484.g006", "stats"=>{"downloads"=>0, "page_views"=>26, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Adhesion_inhibition_by_BJ_treatment_/683190", "title"=>"Adhesion inhibition by BJ treatment.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-16 00:53:10"}
  • {"files"=>["https://ndownloader.figshare.com/files/1025412"], "description"=>"<p>(A) Cytofluorimetric evaluation of apoptosis; representative Annexin V <i>versus</i> PI dot plot analyses of SH-SY5Y cells treated with BJ for 72 hs. (B) Mean percentage of events in each quarter (Q) of cytofluorimetric analyses: Q3 contains the vital cells, Q4 the cells in early apoptosis, Q2 the cells in late apoptosis and Q1 the necrotic cells. The FACS analysis presented is representative of three different experiments. (C) Analysis of caspase-3 and caspase-9 expression in SH-SY5Y cells treated for 72 hs with BJ or etoposide (eto). Lower molecular weight bands of caspases represent the active form. Data show the lack of apoptosis after 72 hs of exposure to BJ.</p>", "links"=>[], "tags"=>["Molecular cell biology", "cell adhesion", "ncam", "Cell growth", "Signal transduction", "Drugs and devices", "Pharmacodynamics", "oncology", "Basic cancer research", "metastasis", "Cancer treatment", "Complementary and alternative medicine", "Cancers and neoplasms", "Neurological tumors", "Oncology agents", "apoptosis", "sh-sy5y", "cells", "exposed"], "article_id"=>683187, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Simona Delle Monache", "Patrizia Sanità", "Elena Trapasso", "Maria Rita Ursino", "Paola Dugo", "Marina Russo", "Nadia Ferlazzo", "Gioacchino Calapai", "Adriano Angelucci", "Michele Navarra"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061484.g004", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Evaluation_of_apoptosis_on_the_SH_SY5Y_cells_exposed_to_BJ_/683187", "title"=>"Evaluation of apoptosis on the SH-SY5Y cells exposed to BJ.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-16 00:53:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/1025424"], "description"=>"*<p>n.a.: standard was not available in sufficient amount for quantitative calculation.</p>", "links"=>[], "tags"=>["Molecular cell biology", "cell adhesion", "ncam", "Cell growth", "Signal transduction", "Drugs and devices", "Pharmacodynamics", "oncology", "Basic cancer research", "metastasis", "Cancer treatment", "Complementary and alternative medicine", "Cancers and neoplasms", "Neurological tumors", "Oncology agents", "flavonoids", "bergamot"], "article_id"=>683199, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Simona Delle Monache", "Patrizia Sanità", "Elena Trapasso", "Maria Rita Ursino", "Paola Dugo", "Marina Russo", "Nadia Ferlazzo", "Gioacchino Calapai", "Adriano Angelucci", "Michele Navarra"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061484.t001", "stats"=>{"downloads"=>4, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Concentration_mg_L_of_flavonoids_in_bergamot_juice_/683199", "title"=>"Concentration (mg/L) of flavonoids in bergamot juice.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-04-16 00:53:19"}

PMC Usage Stats | Further Information

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Relative Metric

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