Novel α-MSH Peptide Analogues with Broad Spectrum Antimicrobial Activity
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Novel α-MSH Peptide Analogues with Broad Spectrum Antimicrobial Activity", "type"=>"journal", "authors"=>[{"first_name"=>"Paolo", "last_name"=>"Grieco", "scopus_author_id"=>"7005897888"}, {"first_name"=>"Alfonso", "last_name"=>"Carotenuto", "scopus_author_id"=>"55866727300"}, {"first_name"=>"Luigia", "last_name"=>"Auriemma", "scopus_author_id"=>"6506122455"}, {"first_name"=>"Antonio", "last_name"=>"Limatola", "scopus_author_id"=>"55630327600"}, {"first_name"=>"Salvatore", "last_name"=>"Di Maro", "scopus_author_id"=>"25647165900"}, {"first_name"=>"Francesco", "last_name"=>"Merlino", "scopus_author_id"=>"55628801100"}, {"first_name"=>"Maria Luisa", "last_name"=>"Mangoni", "scopus_author_id"=>"7003873029"}, {"first_name"=>"Vincenzo", "last_name"=>"Luca", "scopus_author_id"=>"36961342800"}, {"first_name"=>"Antonio", "last_name"=>"Di Grazia", "scopus_author_id"=>"17134927700"}, {"first_name"=>"Stefano", "last_name"=>"Gatti", "scopus_author_id"=>"7103372750"}, {"first_name"=>"Pietro", "last_name"=>"Campiglia", "scopus_author_id"=>"6603015488"}, {"first_name"=>"Isabel", "last_name"=>"Gomez-Monterrey", "scopus_author_id"=>"22134286300"}, {"first_name"=>"Ettore", "last_name"=>"Novellino", "scopus_author_id"=>"7005912361"}, {"first_name"=>"Anna", "last_name"=>"Catania", "scopus_author_id"=>"7102301520"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84876568118", "sgr"=>"84876568118", "pui"=>"368793001", "pmid"=>"23626703", "doi"=>"10.1371/journal.pone.0061614"}, "id"=>"763b5033-4892-3395-a5ab-38e70bc7e99d", "abstract"=>"Previous investigations indicate that α-melanocyte-stimulating hormone (α-MSH) and certain synthetic analogues of it exert antimicrobial effects against bacteria and yeasts. However, these molecules have weak activity in standard microbiology conditions and this hampers a realistic clinical use. The aim in the present study was to identify novel peptides with broad-spectrum antimicrobial activity in growth medium. To this purpose, the Gly10 residue in the [DNal(2')-7, Phe-12]-MSH(6-13) sequence was replaced with conventional and unconventional amino acids with different degrees of conformational rigidity. Two derivatives in which Gly10 was replaced by the residues Aic and Cha, respectively, had substantial activity against Candida strains, including C. albicans, C. glabrata, and C. krusei and against gram-positive and gram-negative bacteria. Conformational analysis indicated that the helical structure along residues 8-13 is a key factor in antimicrobial activity. Synthetic analogues of α-MSH can be valuable agents to treat infections in humans. The structural preferences associated with antimicrobial activity identified in this research can help further development of synthetic melanocortins with enhanced biological activity.", "link"=>"http://www.mendeley.com/research/novel-%CE%B1msh-peptide-analogues-broad-spectrum-antimicrobial-activity", "reader_count"=>13, "reader_count_by_academic_status"=>{"Unspecified"=>3, "Researcher"=>3, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>1, "Student > Bachelor"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>3, "Researcher"=>3, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>1, "Student > Bachelor"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>3, "Pharmacology, Toxicology and Pharmaceutical Science"=>2, "Chemistry"=>3}, "reader_count_by_subdiscipline"=>{"Chemistry"=>{"Chemistry"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>3}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>3}, "Environmental Science"=>{"Environmental Science"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>2}}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84876568118"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84876568118?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84876568118&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84876568118&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84876568118", "dc:identifier"=>"SCOPUS_ID:84876568118", "eid"=>"2-s2.0-84876568118", "dc:title"=>"Novel α-MSH Peptide Analogues with Broad Spectrum Antimicrobial Activity", "dc:creator"=>"Grieco P.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"8", "prism:issueIdentifier"=>"4", "prism:pageRange"=>nil, "prism:coverDate"=>"2013-04-23", "prism:coverDisplayDate"=>"23 April 2013", "prism:doi"=>"10.1371/journal.pone.0061614", "citedby-count"=>"22", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Università degli Studi di Napoli Federico II", "affiliation-city"=>"Naples", "affiliation-country"=>"Italy"}], "pubmed-id"=>"23626703", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e61614", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0061614", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"20", "xml_views"=>"6", "html_views"=>"41"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"34", "xml_views"=>"3", "html_views"=>"92"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"12", "xml_views"=>"3", "html_views"=>"54"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"34"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"46"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"2", "html_views"=>"43"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"44"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"35"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"59"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"48"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"49"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"36"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"46"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"38", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"3", "html_views"=>"27"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"3", "html_views"=>"5"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"4", "html_views"=>"4"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"4", "html_views"=>"10"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"5"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1032268"], "description"=>"<p>Lead sequence <i>DNal</i> and Gly10 substituted peptides.</p>", "links"=>[], "tags"=>["biotechnology", "drug discovery", "Computational biology", "Macromolecular structure analysis", "microbiology", "Mycology", "yeast", "bacteriology", "proteomics", "Synthetic peptide", "medicinal chemistry", "Drugs and devices", "Drug research and development", "Pharmacodynamics", "gly10", "substituted"], "article_id"=>688545, "categories"=>["Medicine", "Chemistry", "Biological Sciences"], "users"=>["Paolo Grieco", "Alfonso Carotenuto", "Luigia Auriemma", "Antonio Limatola", "Salvatore Di Maro", "Francesco Merlino", "Maria Luisa Mangoni", "Vincenzo Luca", "Antonio Di Grazia", "Stefano Gatti", "Pietro Campiglia", "Isabel Gomez-Monterrey", "Ettore Novellino", "Anna Catania"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061614.t001", "stats"=>{"downloads"=>9, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Lead_sequence_DNal_and_Gly10_substituted_peptides_/688545", "title"=>"Lead sequence <i>DNal</i> and Gly10 substituted peptides.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-04-23 02:22:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/1032269"], "description"=>"<p>Each MIC value is the average of at least three independent experiments.</p>", "links"=>[], "tags"=>["biotechnology", "drug discovery", "Computational biology", "Macromolecular structure analysis", "microbiology", "Mycology", "yeast", "bacteriology", "proteomics", "Synthetic peptide", "medicinal chemistry", "Drugs and devices", "Drug research and development", "Pharmacodynamics", "gly10", "substituted", "peptides", "mic", "90", "48"], "article_id"=>688546, "categories"=>["Medicine", "Chemistry", "Biological Sciences"], "users"=>["Paolo Grieco", "Alfonso Carotenuto", "Luigia Auriemma", "Antonio Limatola", "Salvatore Di Maro", "Francesco Merlino", "Maria Luisa Mangoni", "Vincenzo Luca", "Antonio Di Grazia", "Stefano Gatti", "Pietro Campiglia", "Isabel Gomez-Monterrey", "Ettore Novellino", "Anna Catania"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061614.t002", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Anti_Candida_activity_of_DNal_and_Gly10_substituted_peptides_expressed_as_MIC_90_M_at_48_h_/688546", "title"=>"Anti-Candida activity of <i>DNal</i> and Gly10 substituted peptides expressed as MIC 90 (µM) at 48 h.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-04-23 02:22:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/1032270"], "description"=>"<p>Each MIC value is the average of at least three independent experiments.</p>", "links"=>[], "tags"=>["biotechnology", "drug discovery", "Computational biology", "Macromolecular structure analysis", "microbiology", "Mycology", "yeast", "bacteriology", "proteomics", "Synthetic peptide", "medicinal chemistry", "Drugs and devices", "Drug research and development", "Pharmacodynamics", "peptides", "mic", "100", "18"], "article_id"=>688547, "categories"=>["Medicine", "Chemistry", "Biological Sciences"], "users"=>["Paolo Grieco", "Alfonso Carotenuto", "Luigia Auriemma", "Antonio Limatola", "Salvatore Di Maro", "Francesco Merlino", "Maria Luisa Mangoni", "Vincenzo Luca", "Antonio Di Grazia", "Stefano Gatti", "Pietro Campiglia", "Isabel Gomez-Monterrey", "Ettore Novellino", "Anna Catania"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061614.t003", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Antibacterial_activity_of_selected_peptides_expressed_as_MIC_100_181_M_at_18_h_/688547", "title"=>"Antibacterial activity of selected peptides expressed as MIC 100 (µM) at 18 h.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-04-23 02:22:27"}
  • {"files"=>["https://ndownloader.figshare.com/files/1032271"], "description"=>"<p>Values are the mean of three independent experiments ± SD.</p>", "links"=>[], "tags"=>["biotechnology", "drug discovery", "Computational biology", "Macromolecular structure analysis", "microbiology", "Mycology", "yeast", "bacteriology", "proteomics", "Synthetic peptide", "medicinal chemistry", "Drugs and devices", "Drug research and development", "Pharmacodynamics"], "article_id"=>688548, "categories"=>["Medicine", "Chemistry", "Biological Sciences"], "users"=>["Paolo Grieco", "Alfonso Carotenuto", "Luigia Auriemma", "Antonio Limatola", "Salvatore Di Maro", "Francesco Merlino", "Maria Luisa Mangoni", "Vincenzo Luca", "Antonio Di Grazia", "Stefano Gatti", "Pietro Campiglia", "Isabel Gomez-Monterrey", "Ettore Novellino", "Anna Catania"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061614.t004", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Hemolytic_activity_a_of_selected_peptides_/688548", "title"=>"Hemolytic activity<sup>a</sup> (%) of selected peptides.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-04-23 02:22:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/1032273", "https://ndownloader.figshare.com/files/1032276", "https://ndownloader.figshare.com/files/1032296", "https://ndownloader.figshare.com/files/1032299", "https://ndownloader.figshare.com/files/1032300", "https://ndownloader.figshare.com/files/1032308", "https://ndownloader.figshare.com/files/1032313", "https://ndownloader.figshare.com/files/1032315", "https://ndownloader.figshare.com/files/1032318", "https://ndownloader.figshare.com/files/1032324"], "description"=>"<div><p>Previous investigations indicate that α-melanocyte-stimulating hormone (α-MSH) and certain synthetic analogues of it exert antimicrobial effects against bacteria and yeasts. However, these molecules have weak activity in standard microbiology conditions and this hampers a realistic clinical use. The aim in the present study was to identify novel peptides with broad-spectrum antimicrobial activity in growth medium. To this purpose, the Gly10 residue in the [<i>D</i>Nal(2′)-7, Phe-12]-MSH(6–13) sequence was replaced with conventional and unconventional amino acids with different degrees of conformational rigidity. Two derivatives in which Gly10 was replaced by the residues Aic and Cha, respectively, had substantial activity against Candida strains, including <i>C. albicans</i>, <i>C. glabrata</i>, and <i>C. krusei</i> and against gram-positive and gram-negative bacteria. Conformational analysis indicated that the helical structure along residues 8–13 is a key factor in antimicrobial activity. Synthetic analogues of α-MSH can be valuable agents to treat infections in humans. The structural preferences associated with antimicrobial activity identified in this research can help further development of synthetic melanocortins with enhanced biological activity.</p></div>", "links"=>[], "tags"=>["biotechnology", "drug discovery", "Computational biology", "Macromolecular structure analysis", "microbiology", "Mycology", "yeast", "bacteriology", "proteomics", "Synthetic peptide", "medicinal chemistry", "Drugs and devices", "Drug research and development", "Pharmacodynamics", "peptide", "analogues", "antimicrobial"], "article_id"=>688550, "categories"=>["Medicine", "Chemistry", "Biological Sciences"], "users"=>["Paolo Grieco", "Alfonso Carotenuto", "Luigia Auriemma", "Antonio Limatola", "Salvatore Di Maro", "Francesco Merlino", "Maria Luisa Mangoni", "Vincenzo Luca", "Antonio Di Grazia", "Stefano Gatti", "Pietro Campiglia", "Isabel Gomez-Monterrey", "Ettore Novellino", "Anna Catania"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0061614.s001", "https://dx.doi.org/10.1371/journal.pone.0061614.s002", "https://dx.doi.org/10.1371/journal.pone.0061614.s003", "https://dx.doi.org/10.1371/journal.pone.0061614.s004", "https://dx.doi.org/10.1371/journal.pone.0061614.s005", "https://dx.doi.org/10.1371/journal.pone.0061614.s006", "https://dx.doi.org/10.1371/journal.pone.0061614.s007", "https://dx.doi.org/10.1371/journal.pone.0061614.s008", "https://dx.doi.org/10.1371/journal.pone.0061614.s009", "https://dx.doi.org/10.1371/journal.pone.0061614.s010"], "stats"=>{"downloads"=>72, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Novel_945_MSH_Peptide_Analogues_with_Broad_Spectrum_Antimicrobial_Activity_/688550", "title"=>"Novel α-MSH Peptide Analogues with Broad Spectrum Antimicrobial Activity", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-04-23 02:22:30"}
  • {"files"=>["https://ndownloader.figshare.com/files/1032260"], "description"=>"<p>Structures were superimposed using the backbone heavy atoms. Heavy atoms are shown in yellow (except for nitrogen, in blue). To improve clarity, hydrogen atoms are not shown. Backbone atoms of the lowest energy conformer are denoted as a ribbon.</p>", "links"=>[], "tags"=>["biotechnology", "drug discovery", "Computational biology", "Macromolecular structure analysis", "microbiology", "Mycology", "yeast", "bacteriology", "proteomics", "Synthetic peptide", "medicinal chemistry", "Drugs and devices", "Drug research and development", "Pharmacodynamics", "superimposition", "10", "lowest", "conformers", "peptide"], "article_id"=>688539, "categories"=>["Medicine", "Chemistry", "Biological Sciences"], "users"=>["Paolo Grieco", "Alfonso Carotenuto", "Luigia Auriemma", "Antonio Limatola", "Salvatore Di Maro", "Francesco Merlino", "Maria Luisa Mangoni", "Vincenzo Luca", "Antonio Di Grazia", "Stefano Gatti", "Pietro Campiglia", "Isabel Gomez-Monterrey", "Ettore Novellino", "Anna Catania"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061614.g002", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Stereoview_of_the_superimposition_of_the_10_lowest_energy_conformers_of_peptide_8_a_10_b_and_DNal_c_in_SDS_DPC_8_2_solution_/688539", "title"=>"Stereoview of the superimposition of the 10 lowest energy conformers of peptide 8 (a), 10 (b), and <b><i>DNal</i></b><b> (c) in SDS/DPC 8:2 solution.</b>", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-23 02:22:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1032242"], "description"=>"<p>Cells were plated in wells of a microtiter plate, at 4×10<sup>4</sup> cells/well in DMEM supplemented with 2% serum without antibiotic. After overnight incubation at 37°C in a 5% CO<sub>2</sub> atmosphere, the medium was replaced with 100 µl fresh DMEM supplemented with the peptides at different concentrations. After 24 h of peptide treatment, cell viability was determined by the inhibition of MTT reduction to insoluble formazan (see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061614#s2\" target=\"_blank\">Materials and Methods</a> for additional information). Cell viability is expressed as percentage of viability in control cells (cells not treated with the peptide).</p>", "links"=>[], "tags"=>["biotechnology", "drug discovery", "Computational biology", "Macromolecular structure analysis", "microbiology", "Mycology", "yeast", "bacteriology", "proteomics", "Synthetic peptide", "medicinal chemistry", "Drugs and devices", "Drug research and development", "Pharmacodynamics", "synthetic", "melanocortins", "viability", "hacat"], "article_id"=>688531, "categories"=>["Medicine", "Chemistry", "Biological Sciences"], "users"=>["Paolo Grieco", "Alfonso Carotenuto", "Luigia Auriemma", "Antonio Limatola", "Salvatore Di Maro", "Francesco Merlino", "Maria Luisa Mangoni", "Vincenzo Luca", "Antonio Di Grazia", "Stefano Gatti", "Pietro Campiglia", "Isabel Gomez-Monterrey", "Ettore Novellino", "Anna Catania"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061614.g001", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_the_synthetic_melanocortins_on_viability_of_HaCat_cells_/688531", "title"=>"Effects of the synthetic melanocortins on viability of HaCat cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-23 02:22:11"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"1", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"4"}
  • {"unique-ip"=>"48", "full-text"=>"44", "pdf"=>"17", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"102", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"19", "full-text"=>"18", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"50", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"20", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"20", "full-text"=>"18", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"16", "full-text"=>"17", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"17", "full-text"=>"18", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"13", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"14", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"20", "full-text"=>"18", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"14", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"15", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"1", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"22", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"14", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}

Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[269, 466, 588, 697, 800, 896, 988, 1076, 1165, 1254, 1340, 1417]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[284, 491, 620, 738, 843, 945, 1043, 1137, 1225, 1315, 1400, 1479, 1555]}, {"subject_area"=>"/Biology and life sciences/Microbiology", "average_usage"=>[293, 503, 638, 755, 861, 960, 1056, 1146, 1239, 1323, 1403, 1491, 1568]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[272, 466, 589, 702, 806, 903, 995, 1086, 1176, 1258, 1347, 1422, 1493]}, {"subject_area"=>"/Biology and life sciences/Synthetic biology", "average_usage"=>[310, 547, 704, 821, 964, 1059, 1193, 1351, 1449, 1543, 1632, 1722, 1811, 1876]}, {"subject_area"=>"/Engineering and technology", "average_usage"=>[254, 440, 558, 675, 785, 883, 972, 1061, 1154, 1248, 1336, 1414, 1489]}, {"subject_area"=>"/Engineering and technology/Synthetic biology", "average_usage"=>[301, 547, 704, 844, 945, 1059, 1193, 1351, 1449, 1543, 1646, 1755, 1828, 1923]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[264, 460, 584, 692, 794, 887, 978, 1067, 1154, 1241, 1328, 1408, 1474]}, {"subject_area"=>"/Medicine and health sciences/Pharmacology", "average_usage"=>[265, 471, 604, 723, 827, 926, 1022, 1113, 1210, 1290, 1377, 1454, 1532]}, {"subject_area"=>"/Physical sciences/Chemistry", "average_usage"=>[247, 429, 544, 647, 747, 842, 929, 1012, 1099, 1179, 1263, 1339, 1409]}]}
Loading … Spinner
There are currently no alerts