The Motor Protein KIF14 Inhibits Tumor Growth and Cancer Metastasis in Lung Adenocarcinoma
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Mendeley | Further Information

{"title"=>"The Motor Protein KIF14 Inhibits Tumor Growth and Cancer Metastasis in Lung Adenocarcinoma", "type"=>"journal", "authors"=>[{"first_name"=>"Pei-Fang", "last_name"=>"Hung"}, {"first_name"=>"Tse-Ming", "last_name"=>"Hong"}, {"first_name"=>"Yi-Chiung", "last_name"=>"Hsu"}, {"first_name"=>"Hsuan-Yu", "last_name"=>"Chen"}, {"first_name"=>"Yih-Leong", "last_name"=>"Chang"}, {"first_name"=>"Chen-Tu", "last_name"=>"Wu"}, {"first_name"=>"Gee-Chen", "last_name"=>"Chang"}, {"first_name"=>"Yuh-Shan", "last_name"=>"Jou"}, {"first_name"=>"Szu-Hua", "last_name"=>"Pan"}, {"first_name"=>"Pan-Chyr", "last_name"=>"Yang"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"1932-6203", "pmid"=>"23626713", "doi"=>"10.1371/journal.pone.0061664"}, "id"=>"2ed7d335-4e41-36d3-8d85-66fcd3dbdb57", "abstract"=>"The motor protein kinesin superfamily proteins (KIFs) are involved in cancer progression. The depletion of one of the KIFs, KIF14, might delay the metaphase-to-anaphase transition, resulting in a binucleated status, which enhances tumor progression; however, the exact correlation between KIF14 and cancer progression remains ambiguous. In this study, using loss of heterozygosity and array comparative genomic hybridization analyses, we observed a 30% loss in the regions surrounding KIF14 on chromosome 1q in lung adenocarcinomas. In addition, the protein expression levels of KIF14 in 122 lung adenocarcinomas also indicated that approximately 30% of adenocarcinomas showed KIF14 down-regulation compared with the expression in the bronchial epithelial cells of adjacent normal counterparts. In addition, the reduced expression of KIF14 mRNA or proteins was correlated with poor overall survival (P = 0.0158 and <0.0001, respectively), and the protein levels were also inversely correlated with metastasis (P<0.0001). The overexpression of KIF14 in lung adenocarcinoma cells inhibited anchorage-independent growth in vitro and xenograft tumor growth in vivo. The overexpression and silencing of KIF14 also inhibited or enhanced cancer cell migration, invasion and adhesion to the extracellular matrix proteins laminin and collagen IV. Furthermore, we detected the adhesion molecules cadherin 11 (CDH11) and melanoma cell adhesion molecule (MCAM) as cargo on KIF14. The overexpression and silencing of KIF14 enhanced or reduced the recruitment of CDH11 in the membrane fraction, suggesting that KIF14 might act through recruiting adhesion molecules to the cell membrane and modulating cell adhesive, migratory and invasive properties. Thus, KIF14 might inhibit tumor growth and cancer metastasis in lung adenocarcinomas.", "link"=>"http://www.mendeley.com/research/motor-protein-kif14-inhibits-tumor-growth-cancer-metastasis-lung-adenocarcinoma-1", "reader_count"=>8, "reader_count_by_academic_status"=>{"Researcher"=>1, "Student > Ph. D. Student"=>3, "Student > Master"=>2, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Researcher"=>1, "Student > Ph. D. Student"=>3, "Student > Master"=>2, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>7}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>7}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}}, "reader_count_by_country"=>{"United States"=>1}, "group_count"=>3}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1032329"], "description"=>"<p>(A) Loss of heterozygosity (LOH) and microsatellite instability (MI) analyses near the location of KIF14 in primary lung adenocarcinoma. Chromosome 1 ideogram showing the microsatellite markers mapped on 1q according to the location database; the patient number and percentage are indicated. (B) The copy-number alteration profiles of chromosome 1. The KIF14 location is indicated within chromosome 1q32.1. (C) The protein expression pattern of KIF14 was examined using immunohistochemistry with anti-KIF14 antibodies in normal bronchial mucosa (left panel) and primary tumor specimens (middle and right panel). Original magnification 200×.</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Cytoskeletal proteins", "genetics", "Human genetics", "Chromosomal disorders", "Chromosomal deletions and duplications", "Cancer genetics", "Molecular cell biology", "cell adhesion", "Cell growth", "oncology", "Cancers and neoplasms", "Lung and intrathoracic tumors", "Adenocarcinoma of the lung", "down-regulation", "kif14", "occurred"], "article_id"=>688593, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Pei-Fang Hung", "Tse-Ming Hong", "Yi-Chiung Hsu", "Hsuan-Yu Chen", "Yih-Leong Chang", "Chen-Tu Wu", "Gee-Chen Chang", "Yuh-Shan Jou", "Szu-Hua Pan", "Pan-Chyr Yang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061664.g001", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_down_regulation_of_KIF14_occurred_in_lung_adenocarcinomas_/688593", "title"=>"The down-regulation of KIF14 occurred in lung adenocarcinomas.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-23 02:23:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1032331"], "description"=>"<p>(A) Low KIF14 mRNA expression was associated with poor overall survival in the patients with lung adenocarcinoma. The KIF14 mRNA levels were measured using real-time quantitative RT-PCR of primary tumor specimens. The median value was used to divide patients into low (red line) and high (blue line) KIF14 expression groups (log rank, P = 0.0158). (B) Low KIF14 protein expression was associated with poor overall survival in an independent cohort of lung adenocarcinoma patients. The patients were divided into two groups according to the immunoreactivity of the KIF14 antibodies. The cut-off value was 70% in tumor sections showing immunoreactivity to the antibodies. Kaplan-Meier analyses were used to estimate the overall survival in the lung adenocarcinoma patients according to KIF14 expression (log rank, P<0.0001). (C) Percentage analysis of the metastatic status in high and low KIF14 expression groups. Patients in both high and low KIF14 expression groups were further divided based on the presence of metastasis; and the numbers of each subgroup are shown as a percentage using statistical analysis (chi-square test, P<0.0001). (D) The KIF14 expression in human lung adenocarcinoma predicted the occurrence of metastasis. Metastasis-free survival curves of 122 lung adenocarcinoma patients stratified by low (red line) and high (blue line) KIF14 protein expression (log rank, P<0.0001).</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Cytoskeletal proteins", "genetics", "Human genetics", "Chromosomal disorders", "Chromosomal deletions and duplications", "Cancer genetics", "Molecular cell biology", "cell adhesion", "Cell growth", "oncology", "Cancers and neoplasms", "Lung and intrathoracic tumors", "Adenocarcinoma of the lung", "kif14", "negatively", "correlated", "outcomes", "adenocarcinoma"], "article_id"=>688594, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Pei-Fang Hung", "Tse-Ming Hong", "Yi-Chiung Hsu", "Hsuan-Yu Chen", "Yih-Leong Chang", "Chen-Tu Wu", "Gee-Chen Chang", "Yuh-Shan Jou", "Szu-Hua Pan", "Pan-Chyr Yang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061664.g002", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_expression_of_KIF14_was_negatively_correlated_with_clinical_outcomes_in_the_lung_adenocarcinoma_patients_/688594", "title"=>"The expression of KIF14 was negatively correlated with clinical outcomes in the lung adenocarcinoma patients.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-23 02:23:14"}
  • {"files"=>["https://ndownloader.figshare.com/files/1032332"], "description"=>"<p>(A) Cell lines constitutively expressing KIF14 were established through the transfection of pCMV-7.1-3-3×Flag-KIF14 into CL1-5 cells. Single colonies were selected using Geneticin, and the KIF14 protein expression was assessed through immunoblotting with anti-Flag antibodies; actin was used as an internal control. The asterisk and arrowhead indicate the non-specific band and Flag-KIF14, respectively. (B) KIF14 expression was knocked down in CL1-0 cells using shRNA lentiviral infection. After selection with puromycin for two weeks, the KIF14 protein expression patterns were assessed through immunoblotting with anti-KIF14 antibodies; actin was used as an internal control. (C and D) Effects of KIF14 overexpression and silencing on cultured tumor cell proliferation. The cell number was calculated at the indicated times after planting. The error bars represent standard deviations of the mean. No significant differences were observed in the proliferation rates between different cell lines by one-way ANOVA analysis (C). (E and F) Anchorage-independent growth of KIF14-overexpressing and -silencing cell lines were assessed by colony formation in soft agar. The error bars represent the standard deviations of three independent experiments performed in triplicate. (G) KIF14 overexpression reduced tumor growth. The mice were injected subcutaneously into the flanks with CL1-5/vector and CL1-5/KIF14#2 cells. Left panel: Final tumor photographs at 35 days post-subcutaneous injection. Right panel: Average tumor volume at 35 days post-subcutaneous injection was calculated, and the animals were subsequently sacrificed. The bars represent the means ± standard deviations of six mice per group (one-way ANOVA, P = 0.0021).</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Cytoskeletal proteins", "genetics", "Human genetics", "Chromosomal disorders", "Chromosomal deletions and duplications", "Cancer genetics", "Molecular cell biology", "cell adhesion", "Cell growth", "oncology", "Cancers and neoplasms", "Lung and intrathoracic tumors", "Adenocarcinoma of the lung", "inhibited", "anchorage-independent"], "article_id"=>688595, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Pei-Fang Hung", "Tse-Ming Hong", "Yi-Chiung Hsu", "Hsuan-Yu Chen", "Yih-Leong Chang", "Chen-Tu Wu", "Gee-Chen Chang", "Yuh-Shan Jou", "Szu-Hua Pan", "Pan-Chyr Yang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061664.g003", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_KIF14_inhibited_anchorage_independent_growth_in_vitro_and_tumor_growth_in_vivo_/688595", "title"=>"KIF14 inhibited anchorage-independent growth <i>in vitro</i> and tumor growth <i>in vivo</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-23 02:23:15"}
  • {"files"=>["https://ndownloader.figshare.com/files/1032333"], "description"=>"<p>(A) CL1-5 cells with permanent KIF14 overexpression and A549 cells transiently infected with lentivirus encoding non-tagged full-length KIF14 were analyzed for the cell migratory ability in a wound-healing assay. The data show the number of migratory cells, and the P values were calculated relative to vector-only controls using Student’s <i>t</i>-test. (B) Wound-healing assay of CL1-0 and H1299 cells with KIF14 silencing. The number of migratory cells was also calculated. (C and D) KIF14 overexpression reduced cell invasion. The invasion ability of stable or transiently infected KIF14 and KIF14-silenced cell lines was measured using a modified Boyden chambers assay. The invading cells were indicated with propidium iodide staining and quantified (n = 3). (E) KIF14 overexpression enhanced cell adhesion. A 96-well plate was coated with laminin and collagen IV. A total of 3×10<sup>4</sup> cells were plated onto a 96-well plate, incubated at 37°C for 1 hour and the adhesive activity was calculated using crystal violate staining. High absorbance at 540 nm indicated enhanced cell adhesion.</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Cytoskeletal proteins", "genetics", "Human genetics", "Chromosomal disorders", "Chromosomal deletions and duplications", "Cancer genetics", "Molecular cell biology", "cell adhesion", "Cell growth", "oncology", "Cancers and neoplasms", "Lung and intrathoracic tumors", "Adenocarcinoma of the lung", "kif14", "inhibited"], "article_id"=>688596, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Pei-Fang Hung", "Tse-Ming Hong", "Yi-Chiung Hsu", "Hsuan-Yu Chen", "Yih-Leong Chang", "Chen-Tu Wu", "Gee-Chen Chang", "Yuh-Shan Jou", "Szu-Hua Pan", "Pan-Chyr Yang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061664.g004", "stats"=>{"downloads"=>0, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Manipulating_the_expression_of_KIF14_inhibited_cell_migration_invasion_and_adhesion_/688596", "title"=>"Manipulating the expression of KIF14 inhibited cell migration, invasion and adhesion.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-23 02:23:16"}
  • {"files"=>["https://ndownloader.figshare.com/files/1032335"], "description"=>"<p>(A) KIF14 associated with CDH11 and MCAM. The lysate of HEK293T cells transfected with the indicated plasmids was used in exogenous immunoprecipitation assays. Immunoblotting was performed with the indicated antibodies. Actin was used as an internal control. (B) The lysate of H460 cells was used in endogenous immunoprecipitation assays. Immunoblotting was performed with the indicated antibodies. (C) The distribution of CDH11 and MCAM changed with the overexpression of KIF14. H1299 cells were co-transfected with HA-CDH11 or HA-MCAM and GFP-KIF14, fixed and hybridized using anti-HA antibodies. The signal was captured using a confocal microscope (original magnification, 1,000×). (D) HEK293T cells were co-transfected with HA-CDH11 and Flag-KIF14 or siKIF14 and subsequently, the membrane fraction was isolated. The protein in the membrane fraction and total cell lysate was analyzed through immunoblotting. The amounts of HA-CDH11 in the membrane fraction were quantified through normalization with the amount in the total cell lysates. Hsp90 was used as a cytosol marker.</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Cytoskeletal proteins", "genetics", "Human genetics", "Chromosomal disorders", "Chromosomal deletions and duplications", "Cancer genetics", "Molecular cell biology", "cell adhesion", "Cell growth", "oncology", "Cancers and neoplasms", "Lung and intrathoracic tumors", "Adenocarcinoma of the lung", "modulated"], "article_id"=>688597, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Pei-Fang Hung", "Tse-Ming Hong", "Yi-Chiung Hsu", "Hsuan-Yu Chen", "Yih-Leong Chang", "Chen-Tu Wu", "Gee-Chen Chang", "Yuh-Shan Jou", "Szu-Hua Pan", "Pan-Chyr Yang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061664.g005", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_KIF14_modulated_the_distribution_of_the_cargo_molecules_/688597", "title"=>"KIF14 modulated the distribution of the cargo molecules.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-23 02:23:17"}
  • {"files"=>["https://ndownloader.figshare.com/files/1032336"], "description"=>"1<p>KIF14 expression was designated as ‘high’ or ‘low’ using “70% immunoreactivity in tumor sections” as the cut-off point (low KIF14 as the referent), and these values were adjusted according to sex (female as the referent vs. male), age and tumor stage (according to TNM classification). The P values (two-sided) were calculated using Pearson’s chi-square test.</p><p>Abbreviations: CI, confidence interval.</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Cytoskeletal proteins", "genetics", "Human genetics", "Chromosomal disorders", "Chromosomal deletions and duplications", "Cancer genetics", "Molecular cell biology", "cell adhesion", "Cell growth", "oncology", "Cancers and neoplasms", "Lung and intrathoracic tumors", "Adenocarcinoma of the lung", "ratios", "patients", "adenocarcinoma", "immunohistochemistry", "staining", "multivariate", "cox", "regression"], "article_id"=>688598, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Pei-Fang Hung", "Tse-Ming Hong", "Yi-Chiung Hsu", "Hsuan-Yu Chen", "Yih-Leong Chang", "Chen-Tu Wu", "Gee-Chen Chang", "Yuh-Shan Jou", "Szu-Hua Pan", "Pan-Chyr Yang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061664.t003", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Hazard_ratios_for_death_from_any_cause_among_patients_with_lung_adenocarcinoma_determined_using_immunohistochemistry_staining_according_to_multivariate_Cox_regression_analysis_1_/688598", "title"=>"Hazard ratios for death (from any cause) among patients with lung adenocarcinoma determined using immunohistochemistry staining according to multivariate Cox regression analysis<sup>1</sup>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-04-23 02:23:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/1032337"], "description"=>"1<p>KIF14 expression was designated as ‘high’ or ‘low’ using “70% immunoreactivity in tumor sections” as the cut-off point. The P values were calculated using a two-sided Pearson’s chi-squared test.</p>2<p>The P values were calculated using Student’s <i>t</i>-test.</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Cytoskeletal proteins", "genetics", "Human genetics", "Chromosomal disorders", "Chromosomal deletions and duplications", "Cancer genetics", "Molecular cell biology", "cell adhesion", "Cell growth", "oncology", "Cancers and neoplasms", "Lung and intrathoracic tumors", "Adenocarcinoma of the lung", "122", "adenocarcinomas", "immunohistochemistry"], "article_id"=>688599, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Pei-Fang Hung", "Tse-Ming Hong", "Yi-Chiung Hsu", "Hsuan-Yu Chen", "Yih-Leong Chang", "Chen-Tu Wu", "Gee-Chen Chang", "Yuh-Shan Jou", "Szu-Hua Pan", "Pan-Chyr Yang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061664.t002", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Characteristics_of_the_122_lung_adenocarcinomas_determined_using_immunohistochemistry_staining_1_/688599", "title"=>"Characteristics of the 122 lung adenocarcinomas determined using immunohistochemistry staining<sup>1</sup>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-04-23 02:23:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1032338"], "description"=>"1<p>There are two probes (chr1∶198737634–198793986/chr1∶198800168-198856322 [HG18]) located in KIF14.</p>2<p>0 denotes that the immunoreactivity of KIF14 antibodies was almost equal between normal bronchial mucosa and tumors, 1 denotes that the immunoreactivity was up-regulated in tumors compared with normal bronchial mucosa and 2 denotes that the immunoreactivity was down-regulated in tumors compared with normal bronchial mucosa.</p><p>Abbreviations: CGH, comparative genomic hybridization.</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Cytoskeletal proteins", "genetics", "Human genetics", "Chromosomal disorders", "Chromosomal deletions and duplications", "Cancer genetics", "Molecular cell biology", "cell adhesion", "Cell growth", "oncology", "Cancers and neoplasms", "Lung and intrathoracic tumors", "Adenocarcinoma of the lung", "cgh", "kif14", "adenocarcinomas", "adjacent", "bronchial"], "article_id"=>688600, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Pei-Fang Hung", "Tse-Ming Hong", "Yi-Chiung Hsu", "Hsuan-Yu Chen", "Yih-Leong Chang", "Chen-Tu Wu", "Gee-Chen Chang", "Yuh-Shan Jou", "Szu-Hua Pan", "Pan-Chyr Yang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0061664.t001", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_array_CGH_and_KIF14_protein_expression_in_lung_adenocarcinomas_and_adjacent_normal_bronchial_epithelia_/688600", "title"=>"The array CGH and KIF14 protein expression in lung adenocarcinomas and adjacent normal bronchial epithelia.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-04-23 02:23:20"}
  • {"files"=>["https://ndownloader.figshare.com/files/1032339", "https://ndownloader.figshare.com/files/1032340", "https://ndownloader.figshare.com/files/1032341", "https://ndownloader.figshare.com/files/1032342", "https://ndownloader.figshare.com/files/1032343", "https://ndownloader.figshare.com/files/1032344", "https://ndownloader.figshare.com/files/1032345"], "description"=>"<div><p></p><p>The motor protein kinesin superfamily proteins (KIFs) are involved in cancer progression. The depletion of one of the KIFs, KIF14, might delay the metaphase-to-anaphase transition, resulting in a binucleated status, which enhances tumor progression; however, the exact correlation between KIF14 and cancer progression remains ambiguous. In this study, using loss of heterozygosity and array comparative genomic hybridization analyses, we observed a 30% loss in the regions surrounding KIF14 on chromosome 1q in lung adenocarcinomas. In addition, the protein expression levels of KIF14 in 122 lung adenocarcinomas also indicated that approximately 30% of adenocarcinomas showed KIF14 down-regulation compared with the expression in the bronchial epithelial cells of adjacent normal counterparts. In addition, the reduced expression of KIF14 mRNA or proteins was correlated with poor overall survival (P = 0.0158 and <0.0001, respectively), and the protein levels were also inversely correlated with metastasis (P<0.0001). The overexpression of KIF14 in lung adenocarcinoma cells inhibited anchorage-independent growth <i>in vitro</i> and xenograft tumor growth <i>in vivo</i>. The overexpression and silencing of KIF14 also inhibited or enhanced cancer cell migration, invasion and adhesion to the extracellular matrix proteins laminin and collagen IV. Furthermore, we detected the adhesion molecules cadherin 11 (CDH11) and melanoma cell adhesion molecule (MCAM) as cargo on KIF14. The overexpression and silencing of KIF14 enhanced or reduced the recruitment of CDH11 in the membrane fraction, suggesting that KIF14 might act through recruiting adhesion molecules to the cell membrane and modulating cell adhesive, migratory and invasive properties. Thus, KIF14 might inhibit tumor growth and cancer metastasis in lung adenocarcinomas.</p></div>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Cytoskeletal proteins", "genetics", "Human genetics", "Chromosomal disorders", "Chromosomal deletions and duplications", "Cancer genetics", "Molecular cell biology", "cell adhesion", "Cell growth", "oncology", "Cancers and neoplasms", "Lung and intrathoracic tumors", "Adenocarcinoma of the lung", "kif14", "inhibits", "cancer", "metastasis"], "article_id"=>688601, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Pei-Fang Hung", "Tse-Ming Hong", "Yi-Chiung Hsu", "Hsuan-Yu Chen", "Yih-Leong Chang", "Chen-Tu Wu", "Gee-Chen Chang", "Yuh-Shan Jou", "Szu-Hua Pan", "Pan-Chyr Yang"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0061664.s001", "https://dx.doi.org/10.1371/journal.pone.0061664.s002", "https://dx.doi.org/10.1371/journal.pone.0061664.s003", "https://dx.doi.org/10.1371/journal.pone.0061664.s004", "https://dx.doi.org/10.1371/journal.pone.0061664.s005", "https://dx.doi.org/10.1371/journal.pone.0061664.s006", "https://dx.doi.org/10.1371/journal.pone.0061664.s007"], "stats"=>{"downloads"=>2, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_Motor_Protein_KIF14_Inhibits_Tumor_Growth_and_Cancer_Metastasis_in_Lung_Adenocarcinoma_/688601", "title"=>"The Motor Protein KIF14 Inhibits Tumor Growth and Cancer Metastasis in Lung Adenocarcinoma", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-04-23 02:23:21"}

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Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[269, 466, 588, 697, 800, 896, 988, 1076, 1165, 1254, 1340, 1417]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[272, 472, 600, 713, 815, 911, 1004, 1094, 1185, 1273, 1358, 1441]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[264, 460, 584, 692, 794, 887, 978, 1067, 1154, 1241, 1328, 1408, 1474]}]}
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