Stem Cells from Wildtype and Friedreich’s Ataxia Mice Present Similar Neuroprotective Properties in Dorsal Root Ganglia Cells
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{"title"=>"Stem Cells from Wildtype and Friedreich's Ataxia Mice Present Similar Neuroprotective Properties in Dorsal Root Ganglia Cells", "type"=>"journal", "authors"=>[{"first_name"=>"Jonathan", "last_name"=>"Jones", "scopus_author_id"=>"57196738062"}, {"first_name"=>"Alicia", "last_name"=>"Estirado", "scopus_author_id"=>"14024173300"}, {"first_name"=>"Carolina", "last_name"=>"Redondo", "scopus_author_id"=>"15748718700"}, {"first_name"=>"Salvador", "last_name"=>"Martinez", "scopus_author_id"=>"7202228062"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"23671637", "sgr"=>"84877340904", "doi"=>"10.1371/journal.pone.0062807", "scopus"=>"2-s2.0-84877340904", "pui"=>"368882629", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "issn"=>"19326203"}, "id"=>"434637c6-a91d-3f80-be57-744a3723da68", "abstract"=>"Many neurodegenerative disorders share a common susceptibility to oxidative stress, including Alzheimer's, Parkinson Disease, Huntington Disease and Friedreich's ataxia. In a previous work, we proved that stem cell-conditioned medium increased the survival of cells isolated from Friedreich's ataxia patients, when submitted to oxidative stress. The aim of the present work is to confirm this same effect in dorsal root ganglia cells isolated from YG8 mice, a mouse model of Friedreich's ataxia. In this disorder, the neurons of the dorsal root ganglia are the first to degenerate. Also, in this work we cultured mesenchymal stem cells isolated from YG8 mice, in order to compare them with their wildtype counterpart. To this end, dorsal root ganglia primary cultures isolated from YG8 mice were exposed to oxidative stress and cultured with conditioned medium from either wildtype or YG8 stem cells. As a result, the conditioned medium increased the survival of the dorsal root ganglia cells. This coincided with an increase in oxidative stress-related markers and frataxin expression levels. BDNF, NT3 and NT4 trophic factors were detected in the conditioned medium of both wild-type and YG8 stem cells, all which bind to the various neuronal cell types present in the dorsal root ganglia. No differences were observed in the stem cells isolated from wildtype and YG8 mice. The results presented confirm the possibility that autologous stem cell transplantation may be a viable therapeutic approach in protecting dorsal root ganglia neurons of Friedreich's ataxia patients.", "link"=>"http://www.mendeley.com/research/stem-cells-wildtype-friedreichs-ataxia-mice-present-similar-neuroprotective-properties-dorsal-root-g", "reader_count"=>32, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>3, "Researcher"=>6, "Student > Ph. D. Student"=>9, "Student > Postgraduate"=>3, "Other"=>4, "Student > Master"=>2, "Student > Bachelor"=>1, "Professor"=>3}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>3, "Researcher"=>6, "Student > Ph. D. Student"=>9, "Student > Postgraduate"=>3, "Other"=>4, "Student > Master"=>2, "Student > Bachelor"=>1, "Professor"=>3}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>4, "Agricultural and Biological Sciences"=>14, "Medicine and Dentistry"=>8, "Neuroscience"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Psychology"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>8}, "Neuroscience"=>{"Neuroscience"=>1}, "Psychology"=>{"Psychology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>14}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}, "Unspecified"=>{"Unspecified"=>2}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"Mexico"=>1}, "group_count"=>3}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1058194"], "description"=>"<p>A) Quantitative PCR analysis of dorsal root ganglia cells of wildtype (WT) versus YG8 (AtF) mice under normal culture conditions. B) Quantitative PCR analysis of dorsal root ganglia cells under normal culture medium (Control), exposed to hydrogen peroxide (H2O2) and exposed to hydrogen peroxide with conditioned medium from wildtype and YG8 stem cells (MSC-WT and MSC-AtF, respectively). H2O2 cultures express high Bax levels, indicating apoptosis of surviving cells. DRG cultured with conditioned medium presented a significant increase in the expression levels of frataxin, oxidative stress markers, as well as high Bcl-2 and low Bax levels. N = 6 in all cases except for MSC-AtF where n = 4, *p<0.05, **p<0.01, ***p<0.001. C) Western blot analysis of frataxin in the various culture conditions (n = 4). Two bands were observed, one at 30 kDa corresponding to the precursor form of frataxin, and one at 18 kDa corresponding to the mature form.</p>", "links"=>[], "tags"=>["developmental biology", "stem cells", "mesenchymal stem cells", "Model organisms", "Animal models", "mouse", "Molecular cell biology", "Cellular types", "neuroscience", "Neurobiology of disease and regeneration", "neurology", "Movement disorders", "Friedreich ataxia", "dorsal", "ganglia"], "article_id"=>701013, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Jonathan Jones", "Alicia Estirado", "Carolina Redondo", "Salvador Martinez"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0062807.g002", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Quantitative_analysis_of_dorsal_root_ganglia_cells_/701013", "title"=>"Quantitative analysis of dorsal root ganglia cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-05-09 00:16:53"}
  • {"files"=>["https://ndownloader.figshare.com/files/1058192"], "description"=>"<p>(A–D) Dorsal root ganglia primary cultures before (A), 24 hours after exposure to hydrogen peroxide (B), and 24 hours after exposure to hydrogen peroxide and cultured with conditioned medium from wildtype and YG8 stem cells (C and D, respectively). E) The histogram depicts the percentage of live cells in culture among the different culture conditions, considering the control culture as 100%. After being submitted to oxidative stress (labeled H2O2 in the histogram), over 67% of the cells in culture die. However, almost all cells survive when exposed to hydrogen peroxide and under mesenchymal stem cell-conditioned medium. F–I) Immunocytochemistry of Tuj1 (in red) and GFAP (in green) of the dorsal root ganglia cultures. J–K) The histograms present the percentage of cells positive for Tuj1 and GFAP, respectively, in the different cell cultures. The percentages are with respect to the control, normalized to 100%. L) Western blot analysis of Tuj1 and GFAP in the various culture conditions, with beta-actin as control. N = 3 in all cases, *p<0.05, **p<0.01.</p>", "links"=>[], "tags"=>["developmental biology", "stem cells", "mesenchymal stem cells", "Model organisms", "Animal models", "mouse", "Molecular cell biology", "Cellular types", "neuroscience", "Neurobiology of disease and regeneration", "neurology", "Movement disorders", "Friedreich ataxia", "ganglia", "cultures", "exposed", "hydrogen"], "article_id"=>701011, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Jonathan Jones", "Alicia Estirado", "Carolina Redondo", "Salvador Martinez"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0062807.g001", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Dorsal_root_ganglia_cultures_exposed_to_hydrogen_peroxide_/701011", "title"=>"Dorsal root ganglia cultures exposed to hydrogen peroxide.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-05-09 00:16:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/1058197"], "description"=>"<p>A) Quantitative PCR analysis of trophic factors released in the conditioned medium of mesenchymal stem cells isolated from wildtype (WT) and Friedreich’s ataxia mice (AtF) under normal culture conditions. B) Quantitative PCR analysis of trophic factors released in the conditioned medium of mesenchymal stem cells isolated from wildtype (MSC-WT) and Friedreich’s ataxia mice (MSC-AtF), under normal culture conditions (CT) and exposed for 24 hours to hydrogen peroxide (H2O2). N = 6, *p<0.001. C) Western blot analysis of the trophic factors in mesenchymal stem cell-conditioned medium under normal culture conditions and exposed to hydrogen peroxide. P indicates Ponceau S staining as loading control. To the right, gel density analysis of the western blots, where hydrogen peroxide treated stem cells (MSC-WT H2O2 and MSC-AtF H2O2) were compared to their respective control culture condition. N = 4 in all cases, *p<0.05, **p<0.01. D) Quantitative PCR analysis of oxidative stress-related genes, as well as frataxin, of dorsal root ganglia cultures exposed to oxidative stress (CT H2O2) as well as with the stem cell conditioned medium of wildtype (MSC-WT H2O2 K252) and YG8 (MSC ATF H2O2 K252) mice.</p>", "links"=>[], "tags"=>["developmental biology", "stem cells", "mesenchymal stem cells", "Model organisms", "Animal models", "mouse", "Molecular cell biology", "Cellular types", "neuroscience", "Neurobiology of disease and regeneration", "neurology", "Movement disorders", "Friedreich ataxia", "trophic", "nt3", "nt4", "mesenchymal", "cells", "msc-conditioned"], "article_id"=>701015, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Jonathan Jones", "Alicia Estirado", "Carolina Redondo", "Salvador Martinez"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0062807.g003", "stats"=>{"downloads"=>1, "page_views"=>55, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Quantitative_analysis_of_trophic_factors_BDNF_NT3_and_NT4_in_mesenchymal_stem_cells_and_MSC_conditioned_medium_/701015", "title"=>"Quantitative analysis of trophic factors BDNF, NT3 and NT4 in mesenchymal stem cells and MSC-conditioned medium.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-05-09 00:16:55"}

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