Evaluation of Genetically Inactivated Alpha Toxin for Protection in Multiple Mouse Models of Staphylococcus aureus Infection
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Evaluation of Genetically Inactivated Alpha Toxin for Protection in Multiple Mouse Models of Staphylococcus aureus Infection", "type"=>"journal", "authors"=>[{"first_name"=>"Rebecca A.", "last_name"=>"Brady", "scopus_author_id"=>"13007356700"}, {"first_name"=>"Christopher P.", "last_name"=>"Mocca", "scopus_author_id"=>"14054630600"}, {"first_name"=>"Ranjani", "last_name"=>"Prabhakara", "scopus_author_id"=>"8745120700"}, {"first_name"=>"Roger D.", "last_name"=>"Plaut", "scopus_author_id"=>"8234714000"}, {"first_name"=>"Mark E.", "last_name"=>"Shirtliff", "scopus_author_id"=>"6602190365"}, {"first_name"=>"Tod J.", "last_name"=>"Merkel", "scopus_author_id"=>"7003849123"}, {"first_name"=>"Drusilla L.", "last_name"=>"Burns", "scopus_author_id"=>"18338607600"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"368833116", "pmid"=>"23658662", "doi"=>"10.1371/journal.pone.0063040", "sgr"=>"84876920391", "issn"=>"19326203", "scopus"=>"2-s2.0-84876920391", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)"}, "id"=>"531cdaf7-8188-382b-9cd1-9ad60daf1eb7", "abstract"=>"Staphylococcus aureus is a major human pathogen and a leading cause of nosocomial and community-acquired infections. Development of a vaccine against this pathogen is an important goal. While S. aureus protective antigens have been identified in the literature, the majority have only been tested in a single animal model of disease. We wished to evaluate the ability of one S. aureus vaccine antigen to protect in multiple mouse models, thus assessing whether protection in one model translates to protection in other models encompassing the full breadth of infections the pathogen can cause. We chose to focus on genetically inactivated alpha toxin mutant HlaH35L. We evaluated the protection afforded by this antigen in three models of infection using the same vaccine dose, regimen, route of immunization, adjuvant, and challenge strain. When mice were immunized with HlaH35L and challenged via a skin and soft tissue infection model, HlaH35L immunization led to a less severe infection and decreased S. aureus levels at the challenge site when compared to controls. Challenge of HlaH35L-immunized mice using a systemic infection model resulted in a limited, but statistically significant decrease in bacterial colonization as compared to that observed with control mice. In contrast, in a prosthetic implant model of chronic biofilm infection, there was no significant difference in bacterial levels when compared to controls. These results demonstrate that vaccines may confer protection against one form of S. aureus disease without conferring protection against other disease presentations and thus underscore a significant challenge in S. aureus vaccine development.", "link"=>"http://www.mendeley.com/research/evaluation-genetically-inactivated-alpha-toxin-protection-multiple-mouse-models-staphylococcus-aureu", "reader_count"=>27, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Librarian"=>1, "Researcher"=>5, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>2, "Student > Bachelor"=>1, "Professor"=>3}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Librarian"=>1, "Researcher"=>5, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>2, "Student > Bachelor"=>1, "Professor"=>3}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>2, "Materials Science"=>1, "Agricultural and Biological Sciences"=>19, "Medicine and Dentistry"=>2, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>19}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"Canada"=>1}, "group_count"=>3}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84876920391"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84876920391?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84876920391&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84876920391&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84876920391", "dc:identifier"=>"SCOPUS_ID:84876920391", "eid"=>"2-s2.0-84876920391", "dc:title"=>"Evaluation of Genetically Inactivated Alpha Toxin for Protection in Multiple Mouse Models of Staphylococcus aureus Infection", "dc:creator"=>"Brady R.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"8", "prism:issueIdentifier"=>"4", "prism:pageRange"=>nil, "prism:coverDate"=>"2013-04-29", "prism:coverDisplayDate"=>"29 April 2013", "prism:doi"=>"10.1371/journal.pone.0063040", "citedby-count"=>"20", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"FDA Center for Biologics Evaluation and Research", "affiliation-city"=>"Bethesda", "affiliation-country"=>"United States"}], "pubmed-id"=>"23658662", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e63040", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0063040", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"4", "year"=>"2013", "pdf_views"=>"22", "xml_views"=>"17", "html_views"=>"332"}
  • {"month"=>"5", "year"=>"2013", "pdf_views"=>"54", "xml_views"=>"4", "html_views"=>"158"}
  • {"month"=>"6", "year"=>"2013", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"44"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"15", "xml_views"=>"6", "html_views"=>"73"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"22"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"107"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"50"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"3", "html_views"=>"34"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"18"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"27"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"10"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"9"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"4"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"3", "html_views"=>"4"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"4"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1047892"], "description"=>"<p>Photographs were taken on day 14 post-challenge.</p>", "links"=>[], "tags"=>["immunology", "immunity", "microbiology", "Bacterial pathogens", "staphylococci", "bacteriology", "Medical microbiology", "Microbial control", "Microbial pathogens", "Model organisms", "Animal models", "mouse", "Clinical immunology", "vaccination", "vaccines", "Vaccine development", "Clinical research design", "Animal models of disease", "Infectious diseases", "Bacterial diseases", "Staphylococcus aureus", "ssti", "hlah35l-immunized"], "article_id"=>693440, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Rebecca A. Brady", "Christopher P. Mocca", "Ranjani Prabhakara", "Roger D. Plaut", "Mark E. Shirtliff", "Tod J. Merkel", "Drusilla L. Burns"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0063040.g001", "stats"=>{"downloads"=>1, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Assessment_of_tissue_damage_from_SSTI_in_control_vs_HlaH35L_immunized_mice_/693440", "title"=>"Assessment of tissue damage from SSTI in control vs HlaH35L-immunized mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-29 00:57:20"}
  • {"files"=>["https://ndownloader.figshare.com/files/1047900"], "description"=>"<p>USOE: Upstream outer end; USIE: Upstream inner end; DSOE: Downstream outer end; DSIE: Downstream inner end. Upstream primers were used to generate a PCR product, and downstream primers were used separately to generate a second product. The two products were then ligated together. The codon change from histidine to leucine at position 35 is noted in DSIE (underlined). The six-histidine tag is indicated in DSOE (italics).</p>", "links"=>[], "tags"=>["immunology", "immunity", "microbiology", "Bacterial pathogens", "staphylococci", "bacteriology", "Medical microbiology", "Microbial control", "Microbial pathogens", "Model organisms", "Animal models", "mouse", "Clinical immunology", "vaccination", "vaccines", "Vaccine development", "Clinical research design", "Animal models of disease", "Infectious diseases", "Bacterial diseases", "Staphylococcus aureus"], "article_id"=>693445, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Rebecca A. Brady", "Christopher P. Mocca", "Ranjani Prabhakara", "Roger D. Plaut", "Mark E. Shirtliff", "Tod J. Merkel", "Drusilla L. Burns"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0063040.t001", "stats"=>{"downloads"=>3, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Primers_used_in_this_study_/693445", "title"=>"Primers used in this study.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-04-29 00:57:25"}
  • {"files"=>["https://ndownloader.figshare.com/files/1047895"], "description"=>"<p>Control animals receiving aluminum hydroxide/CpG adjuvant (•) and animals receiving HlaH35L with adjuvant (▪) were compared in an IV challenge model with 3×10<sup>6</sup> CFU SAP149 as the challenge dose. Shown are the data for two individual experiments performed identically (A and B) and the combined data (C). The CFU per pair of kidneys are plotted, with the mean +/− SEM indicated for each group and the <i>P</i> value determined by two-tailed Student’s t-test. The dashed line indicates the limit of detection.</p>", "links"=>[], "tags"=>["immunology", "immunity", "microbiology", "Bacterial pathogens", "staphylococci", "bacteriology", "Medical microbiology", "Microbial control", "Microbial pathogens", "Model organisms", "Animal models", "mouse", "Clinical immunology", "vaccination", "vaccines", "Vaccine development", "Clinical research design", "Animal models of disease", "Infectious diseases", "Bacterial diseases", "Staphylococcus aureus", "hlah35l", "systemic"], "article_id"=>693443, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Rebecca A. Brady", "Christopher P. Mocca", "Ranjani Prabhakara", "Roger D. Plaut", "Mark E. Shirtliff", "Tod J. Merkel", "Drusilla L. Burns"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0063040.g003", "stats"=>{"downloads"=>1, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Analysis_of_HlaH35L_in_a_systemic_infection_model_of_S_aureus_infection_/693443", "title"=>"Analysis of HlaH35L in a systemic infection model of <i>S. aureus</i> infection.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-29 00:57:23"}
  • {"files"=>["https://ndownloader.figshare.com/files/1047898"], "description"=>"<p>Control animals receiving aluminum hydroxide with CpG adjuvant (•) and animals receiving HlaH35L with adjuvant (▪) were compared. Shown are the results for two experiments performed identically (A and B). The CFU per gram of bone are plotted, with the mean +/− SEM indicated for each group.</p>", "links"=>[], "tags"=>["immunology", "immunity", "microbiology", "Bacterial pathogens", "staphylococci", "bacteriology", "Medical microbiology", "Microbial control", "Microbial pathogens", "Model organisms", "Animal models", "mouse", "Clinical immunology", "vaccination", "vaccines", "Vaccine development", "Clinical research design", "Animal models of disease", "Infectious diseases", "Bacterial diseases", "Staphylococcus aureus", "hlah35l", "prosthetic", "implant"], "article_id"=>693444, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Rebecca A. Brady", "Christopher P. Mocca", "Ranjani Prabhakara", "Roger D. Plaut", "Mark E. Shirtliff", "Tod J. Merkel", "Drusilla L. Burns"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0063040.g004", "stats"=>{"downloads"=>4, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Analysis_of_HlaH35L_in_a_prosthetic_implant_model_of_S_aureus_infection_/693444", "title"=>"Analysis of HlaH35L in a prosthetic implant model of <i>S. aureus</i> infection.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-29 00:57:24"}
  • {"files"=>["https://ndownloader.figshare.com/files/1047893"], "description"=>"<p>Control animals receiving aluminum hydroxide/CpG adjuvant (•) and animals receiving HlaH35L with adjuvant (▪) were compared at days four (A), seven (B), and fourteen (C) post-challenge. The CFU per ear are plotted, with the mean +/− SEM indicated for each group and <i>P</i> value calculated by two-tailed Student’s t test. The dashed line indicates the limit of detection.</p>", "links"=>[], "tags"=>["immunology", "immunity", "microbiology", "Bacterial pathogens", "staphylococci", "bacteriology", "Medical microbiology", "Microbial control", "Microbial pathogens", "Model organisms", "Animal models", "mouse", "Clinical immunology", "vaccination", "vaccines", "Vaccine development", "Clinical research design", "Animal models of disease", "Infectious diseases", "Bacterial diseases", "Staphylococcus aureus", "hlah35l"], "article_id"=>693441, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Rebecca A. Brady", "Christopher P. Mocca", "Ranjani Prabhakara", "Roger D. Plaut", "Mark E. Shirtliff", "Tod J. Merkel", "Drusilla L. Burns"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0063040.g002", "stats"=>{"downloads"=>0, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Analysis_of_HlaH35L_in_a_skin_and_soft_tissue_infection_model_of_S_aureus_infection_/693441", "title"=>"Analysis of HlaH35L in a skin and soft tissue infection model of <i>S. aureus</i> infection.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-04-29 00:57:21"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"38", "full-text"=>"41", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"5"}
  • {"unique-ip"=>"33", "full-text"=>"32", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"6"}
  • {"unique-ip"=>"29", "full-text"=>"33", "pdf"=>"21", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"23", "full-text"=>"19", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"21", "full-text"=>"24", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"6", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"16", "full-text"=>"19", "pdf"=>"9", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"19", "full-text"=>"17", "pdf"=>"10", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"19", "full-text"=>"35", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"20", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"19", "full-text"=>"33", "pdf"=>"12", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"19", "full-text"=>"18", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"5", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"14", "full-text"=>"18", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"12", "full-text"=>"23", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"19", "full-text"=>"16", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"17", "full-text"=>"20", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"17", "full-text"=>"33", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}

Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[269, 466, 588, 697, 800, 896, 988, 1076, 1165, 1254, 1340, 1417]}, {"subject_area"=>"/Biology and life sciences/Immunology", "average_usage"=>[266, 466, 591, 701, 799, 887, 982, 1067, 1155, 1237, 1317, 1395, 1458]}, {"subject_area"=>"/Biology and life sciences/Organisms", "average_usage"=>[281, 484, 611, 728, 835, 934, 1030, 1123, 1214, 1299, 1383, 1464]}, {"subject_area"=>"/Ecology and environmental sciences/Ecology", "average_usage"=>[298, 487, 610, 722, 827, 929, 1029, 1125, 1217, 1306, 1388, 1464, 1535]}, {"subject_area"=>"/Medicine and health sciences/Dermatology", "average_usage"=>[263, 475, 610, 728, 827, 905, 998, 1117, 1223, 1313, 1412, 1514, 1619, 1709]}, {"subject_area"=>"/Medicine and health sciences/Pathology and laboratory medicine", "average_usage"=>[267, 466, 592, 709, 806, 901, 989, 1075, 1162, 1254, 1342, 1424, 1486]}]}
Loading … Spinner
There are currently no alerts