Functional Characterization of Detergent-Decellularized Equine Tendon Extracellular Matrix for Tissue Engineering Applications
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{"title"=>"Functional Characterization of Detergent-Decellularized Equine Tendon Extracellular Matrix for Tissue Engineering Applications", "type"=>"journal", "authors"=>[{"first_name"=>"Daniel W.", "last_name"=>"Youngstrom", "scopus_author_id"=>"35742480300"}, {"first_name"=>"Jennifer G.", "last_name"=>"Barrett", "scopus_author_id"=>"12792954900"}, {"first_name"=>"Rod R.", "last_name"=>"Jose", "scopus_author_id"=>"55331284800"}, {"first_name"=>"David L.", "last_name"=>"Kaplan", "scopus_author_id"=>"34975067900"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84878290280", "sgr"=>"84878290280", "pui"=>"369013308", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"23724028", "doi"=>"10.1371/journal.pone.0064151"}, "id"=>"55bffe9d-3b9b-3870-a69c-b3dd16c08256", "abstract"=>"Natural extracellular matrix provides a number of distinct advantages for engineering replacement orthopedic tissue due to its intrinsic functional properties. The goal of this study was to optimize a biologically derived scaffold for tendon tissue engineering using equine flexor digitorum superficialis tendons. We investigated changes in scaffold composition and ultrastructure in response to several mechanical, detergent and enzymatic decellularization protocols using microscopic techniques and a panel of biochemical assays to evaluate total protein, collagen, glycosaminoglycan, and deoxyribonucleic acid content. Biocompatibility was also assessed with static mesenchymal stem cell (MSC) culture. Implementation of a combination of freeze/thaw cycles, incubation in 2% sodium dodecyl sulfate (SDS), trypsinization, treatment with DNase-I, and ethanol sterilization produced a non-cytotoxic biomaterial free of appreciable residual cellular debris with no significant modification of biomechanical properties. These decellularized tendon scaffolds (DTS) are suitable for complex tissue engineering applications, as they provide a clean slate for cell culture while maintaining native three-dimensional architecture.", "link"=>"http://www.mendeley.com/research/functional-characterization-detergentdecellularized-equine-tendon-extracellular-matrix-tissue-engine", "reader_count"=>71, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>2, "Student > Doctoral Student"=>2, "Researcher"=>11, "Student > Ph. D. Student"=>27, "Student > Postgraduate"=>3, "Student > Master"=>15, "Other"=>3, "Student > Bachelor"=>4, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>2, "Student > Doctoral Student"=>2, "Researcher"=>11, "Student > Ph. D. Student"=>27, "Student > Postgraduate"=>3, "Student > Master"=>15, "Other"=>3, "Student > Bachelor"=>4, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>14, "Unspecified"=>3, "Biochemistry, Genetics and Molecular Biology"=>10, "Nursing and Health Professions"=>1, "Materials Science"=>2, "Medicine and Dentistry"=>9, "Agricultural and Biological Sciences"=>29, "Veterinary Science and Veterinary Medicine"=>3}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>14}, "Materials Science"=>{"Materials Science"=>2}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>9}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>29}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>10}, "Unspecified"=>{"Unspecified"=>3}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>3}}, "reader_count_by_country"=>{"Netherlands"=>1, "United States"=>3, "Brazil"=>1, "United Kingdom"=>3, "France"=>1, "Germany"=>1}, "group_count"=>1}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1068142"], "description"=>"<p>(A) Plating efficiency calculated as a ratio of MSCs adherent to scaffolds following a 48-hour incubation period. (B) Total cell count at 96 hours obtained via MTS assay, indicating no significant reduction in proliferation or metabolic activity on DTS. (C) Fluorescence micrographs portraying representative samples of untreated and decellularized tendon, with live cells stained green (calcein) and dead cells stained red (EthD-1).</p>", "links"=>[], "tags"=>["Anatomy and physiology", "Musculoskeletal system", "biomechanics", "biotechnology", "Tissue engineering", "Molecular cell biology", "Cellular types", "stem cells", "mesenchymal stem cells", "Extracellular matrix", "surgery", "Orthopedic surgery", "msc"], "article_id"=>707221, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Daniel W. Youngstrom", "Jennifer G. Barrett", "Rod R. Jose", "David L. Kaplan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0064151.g004", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Biocompatibility_as_assessed_with_MSC_culture_/707221", "title"=>"Biocompatibility as assessed with MSC culture.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-05-27 02:00:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/1068140"], "description"=>"<p>(A) Scanning electron micrograph of untreated tendon strip, angled to show both longitudinal and transverse section architecture. (B) Histological sections stained with Masson’s trichrome indicate maintenance of collagen content with a slight increase in porosity. (C) Comparison of SEM images obtained from untreated and 2% SDS decellularized tendon samples, shown at 100x and 5000x magnifications longitudinally, and 5000x transversely. Collagen ultrastructure is not adversely altered by detergent treatment.</p>", "links"=>[], "tags"=>["Anatomy and physiology", "Musculoskeletal system", "biomechanics", "biotechnology", "Tissue engineering", "Molecular cell biology", "Cellular types", "stem cells", "mesenchymal stem cells", "Extracellular matrix", "surgery", "Orthopedic surgery"], "article_id"=>707220, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Daniel W. Youngstrom", "Jennifer G. Barrett", "Rod R. Jose", "David L. Kaplan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0064151.g003", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Scaffold_ultrastructure_/707220", "title"=>"Scaffold ultrastructure.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-05-27 02:00:20"}
  • {"files"=>["https://ndownloader.figshare.com/files/1068136"], "description"=>"<p>Brightfield images of H&E-stained histological cross-sections (5 µm-thick) and fluorescence micrographs of scaffolds imaged with EthD-1 (400 µm-thick). Marked loss of DNA content is evident in SDS-decellularized experimental groups, with 2% SDS resulting in the most dramatic decellularization. All images are representative and were acquired from the same horse for ease of comparison.</p>", "links"=>[], "tags"=>["Anatomy and physiology", "Musculoskeletal system", "biomechanics", "biotechnology", "Tissue engineering", "Molecular cell biology", "Cellular types", "stem cells", "mesenchymal stem cells", "Extracellular matrix", "surgery", "Orthopedic surgery"], "article_id"=>707217, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Daniel W. Youngstrom", "Jennifer G. Barrett", "Rod R. Jose", "David L. Kaplan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0064151.g001", "stats"=>{"downloads"=>1, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Loss_of_nuclear_content_following_decellularization_/707217", "title"=>"Loss of nuclear content following decellularization.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-05-27 02:00:17"}
  • {"files"=>["https://ndownloader.figshare.com/files/1068144"], "description"=>"<p>Tensile testing indicated no statistically significant alterations in scaffold mechanics following decellularization. Data represents mean ± standard error values.</p>", "links"=>[], "tags"=>["Anatomy and physiology", "Musculoskeletal system", "biomechanics", "biotechnology", "Tissue engineering", "Molecular cell biology", "Cellular types", "stem cells", "mesenchymal stem cells", "Extracellular matrix", "surgery", "Orthopedic surgery"], "article_id"=>707223, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Daniel W. Youngstrom", "Jennifer G. Barrett", "Rod R. Jose", "David L. Kaplan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0064151.t001", "stats"=>{"downloads"=>2, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_DTS_biomechanics_/707223", "title"=>"DTS biomechanics.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-05-27 02:00:23"}
  • {"files"=>["https://ndownloader.figshare.com/files/1068143"], "description"=>"<p>H&E staining demonstrates infiltration of MSCs deep into DTS following 11 days of static tissue culture.</p>", "links"=>[], "tags"=>["Anatomy and physiology", "Musculoskeletal system", "biomechanics", "biotechnology", "Tissue engineering", "Molecular cell biology", "Cellular types", "stem cells", "mesenchymal stem cells", "Extracellular matrix", "surgery", "Orthopedic surgery"], "article_id"=>707222, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Daniel W. Youngstrom", "Jennifer G. Barrett", "Rod R. Jose", "David L. Kaplan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0064151.g005", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cellular_integration_/707222", "title"=>"Cellular integration.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-05-27 02:00:22"}
  • {"files"=>["https://ndownloader.figshare.com/files/1068138"], "description"=>"<p>Biochemical analysis of tendon scaffolds, including DNA (A), total protein (B), soluble collagen (C), and GAG content (D). Statistical significance between untreated control and treatment is annotated by use of an asterisk. Statistical differences between treatments are indicated by different letters (repeated measures MANOVA).</p>", "links"=>[], "tags"=>["Anatomy and physiology", "Musculoskeletal system", "biomechanics", "biotechnology", "Tissue engineering", "Molecular cell biology", "Cellular types", "stem cells", "mesenchymal stem cells", "Extracellular matrix", "surgery", "Orthopedic surgery", "alters", "tendon", "matrix"], "article_id"=>707218, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Daniel W. Youngstrom", "Jennifer G. Barrett", "Rod R. Jose", "David L. Kaplan"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0064151.g002", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Treatment_alters_tendon_matrix_composition_/707218", "title"=>"Treatment alters tendon matrix composition.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-05-27 02:00:18"}

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