Acyl Chain-Dependent Effect of Lysophosphatidylcholine on Endothelium-Dependent Vasorelaxation
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{"title"=>"Acyl Chain-Dependent Effect of Lysophosphatidylcholine on Endothelium-Dependent Vasorelaxation", "type"=>"journal", "authors"=>[{"first_name"=>"Shailaja P.", "last_name"=>"Rao", "scopus_author_id"=>"56201545000"}, {"first_name"=>"Monika", "last_name"=>"Riederer", "scopus_author_id"=>"7005689259"}, {"first_name"=>"Margarete", "last_name"=>"Lechleitner", "scopus_author_id"=>"55147273200"}, {"first_name"=>"Martin", "last_name"=>"Hermansson", "scopus_author_id"=>"9241690600"}, {"first_name"=>"Gernot", "last_name"=>"Desoye", "scopus_author_id"=>"55129605900"}, {"first_name"=>"Seth", "last_name"=>"Hallström", "scopus_author_id"=>"25021500100"}, {"first_name"=>"Wolfgang F.", "last_name"=>"Graier", "scopus_author_id"=>"7007176734"}, {"first_name"=>"Saša", "last_name"=>"Frank", "scopus_author_id"=>"7202570381"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84878574731", "doi"=>"10.1371/journal.pone.0065155", "pui"=>"369052219", "pmid"=>"20610733", "scopus"=>"2-s2.0-84878574731", "issn"=>"19326203", "isbn"=>"0022-2275"}, "id"=>"2e74c0d0-f1b4-3e65-b0e1-19c62a16cac7", "abstract"=>"Previously we identified palmitoyl-lysophosphatidylcholine (16:0 LPC), linoleoyl-LPC (18:2 LPC), arachidonoyl-LPC (20:4 LPC), and oleoyl-LPC (18:1 LPC) as the most prominent LPC species generated by the action of endothelial lipase (EL) on high-density lipoprotein. In the present study, the impact of those LPC on prostacyclin (PGI(2)) production was examined in vitro in primary human aortic endothelial cells (HAEC) and in vivo in mice. Although 18:2 LPC was inactive, 16:0, 18:1, and 20:4 LPC induced PGI(2) production in HAEC by 1.4-, 3-, and 8.3-fold, respectively. LPC-elicited 6-keto PGF1α formation depended on both cyclooxygenase (COX)-1 and COX-2 and on the activity of cytosolic phospholipase type IVA (cPLA2). The LPC-induced, cPLA2-dependent (14)C-arachidonic acid (AA) release was increased 4.5-fold with 16:0, 2-fold with 18:1, and 2.7-fold with 20:4 LPC, respectively, and related to the ability of LPC to increase cytosolic Ca(2+) concentration. In vivo, LPC increased 6-keto PGF(1α) concentration in mouse plasma with a similar order of potency as found in HAEC. Our results indicate that the tested LPC species are capable of eliciting production of PGI(2), whereby the efficacy and the relative contribution of underlying mechanisms are strongly related to acyl-chain length and degree of saturation.", "link"=>"http://www.mendeley.com/research/acyl-chaindependent-effect-lysophosphatidylcholine-endotheliumdependent-vasorelaxation", "reader_count"=>52, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>2, "Researcher"=>14, "Student > Doctoral Student"=>10, "Student > Ph. D. Student"=>11, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>4, "Professor"=>4}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>2, "Researcher"=>14, "Student > Doctoral Student"=>10, "Student > Ph. D. Student"=>11, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>4, "Professor"=>4}, "reader_count_by_subject_area"=>{"Unspecified"=>6, "Biochemistry, Genetics and Molecular Biology"=>8, "Materials Science"=>1, "Agricultural and Biological Sciences"=>24, "Medicine and Dentistry"=>5, "Pharmacology, Toxicology and Pharmaceutical Science"=>5, "Immunology and Microbiology"=>1, "Engineering"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>5}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>24}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>8}, "Unspecified"=>{"Unspecified"=>6}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>5}}, "reader_count_by_country"=>{"Sweden"=>1, "Belgium"=>1, "United Kingdom"=>1}, "group_count"=>2}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1072331"], "description"=>"<p>The rings were preincubated without (no LPC) or with LPC 20:4 in the absence or presence of 10 µM furegrelate, a TXA<sub>2</sub> synthase inhibitor (A) or 10 µM tranylcypromine, a PGI<sub>2</sub> synthase inhibitor (B) or a combination of both (C) for 30 minutes, followed by precontraction with NE and cumulative addition of ACh. <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0065155#s3\" target=\"_blank\">Results</a> are mean ± SEM of 12 rings from 6 mice. *P<0.05, **P<0.01.</p>", "links"=>[], "tags"=>["Biochemistry", "lipids", "Lipid mediators", "Molecular cell biology", "Signal transduction", "Signaling cascades", "Phospholipid signaling cascade", "Signaling in cellular processes", "Lipid signaling", "cardiovascular", "Cardiovascular pharmacology", "Vascular biology", "synthase", "improves", "relaxation", "attenuated", "lpc"], "article_id"=>710125, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Shailaja P. Rao", "Monika Riederer", "Margarete Lechleitner", "Martin Hermansson", "Gernot Desoye", "Seth Hallström", "Wolfgang F. Graier", "Sasa Frank"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0065155.g004", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Inhibition_of_TXA_2_and_PGI_2_synthase_improves_relaxation_attenuated_by_LPC_20_4_/710125", "title"=>"Inhibition of TXA<sub>2</sub>- and PGI<sub>2</sub>- synthase improves relaxation attenuated by LPC 20:4.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-05-31 00:02:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1072330"], "description"=>"<p>The rings were preincubated without (no LPC) or with 10 µM LPC 18:2 (A) or 20:4 (B) in the absence or presence of 10 µM SQ29548, a TP receptor antagonist for 30 minutes, followed by precontraction with NE and cumulative addition of ACh. <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0065155#s3\" target=\"_blank\">Results</a> are mean ± SEM of 12 rings from 6 mice. *P<0.05, **P<0.01***P<0.001.</p>", "links"=>[], "tags"=>["Biochemistry", "lipids", "Lipid mediators", "Molecular cell biology", "Signal transduction", "Signaling cascades", "Phospholipid signaling cascade", "Signaling in cellular processes", "Lipid signaling", "cardiovascular", "Cardiovascular pharmacology", "Vascular biology", "tp", "receptor", "improves", "lpc", "attenuation"], "article_id"=>710124, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Shailaja P. Rao", "Monika Riederer", "Margarete Lechleitner", "Martin Hermansson", "Gernot Desoye", "Seth Hallström", "Wolfgang F. Graier", "Sasa Frank"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0065155.g003", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Blocking_of_TP_receptor_improves_LPC_18_2_and_20_4_induced_attenuation_of_relaxation_/710124", "title"=>"Blocking of TP receptor improves LPC 18:2- and 20:4-induced attenuation of relaxation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-05-31 00:02:04"}
  • {"files"=>["https://ndownloader.figshare.com/files/1072327"], "description"=>"<p>The rings were preincubated without (no LPC) or with 10 µM LPC 16:0 (A), 18:1 (B), 18:2 (C) or 20:4 (D) for 30 minutes, followed by precontraction with NE and cumulative addition of ACh. Relaxation values were expressed as a percentage of the NE-induced contraction. <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0065155#s3\" target=\"_blank\">Results</a> of each experimental condition are mean ± SEM of 24 rings for each case from 6 mice. *P<0.05, **P<0.01***P<0.001.</p>", "links"=>[], "tags"=>["Biochemistry", "lipids", "Lipid mediators", "Molecular cell biology", "Signal transduction", "Signaling cascades", "Phospholipid signaling cascade", "Signaling in cellular processes", "Lipid signaling", "cardiovascular", "Cardiovascular pharmacology", "Vascular biology", "relaxation", "aortic", "rings", "attenuated"], "article_id"=>710121, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Shailaja P. Rao", "Monika Riederer", "Margarete Lechleitner", "Martin Hermansson", "Gernot Desoye", "Seth Hallström", "Wolfgang F. Graier", "Sasa Frank"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0065155.g001", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ACh_induced_relaxation_of_mouse_aortic_rings_is_attenuated_by_LPC_/710121", "title"=>"ACh-induced relaxation of mouse aortic rings is attenuated by LPC.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-05-31 00:02:01"}
  • {"files"=>["https://ndownloader.figshare.com/files/1072336", "https://ndownloader.figshare.com/files/1072337", "https://ndownloader.figshare.com/files/1072338", "https://ndownloader.figshare.com/files/1072339", "https://ndownloader.figshare.com/files/1072340", "https://ndownloader.figshare.com/files/1072341"], "description"=>"<div><p>Previously we identified palmitoyl-, oleoyl-, linoleoyl-, and arachidonoyl-lysophosphatidylcholine (LPC 16:0, 18:1, 18:2 and 20:4) as the most prominent LPC species generated by endothelial lipase (EL). In the present study, we examined the impact of those LPC on acetylcholine (ACh)- induced vascular relaxation. All tested LPC attenuated ACh-induced relaxation, measured ex vivo, using mouse aortic rings and wire myography. The rank order of potency was as follows: 18:2>20:4>16:0>18:1. The attenuating effect of LPC 16:0 on relaxation was augmented by indomethacin-mediated cyclooxygenase (COX)-inhibition and CAY10441, a prostacyclin (PGI<sub>2</sub>)- receptor (IP) antagonist. Relaxation attenuated by LPC 20:4 and 18:2 was improved by indomethacin and SQ29548, a thromboxane A<sub>2</sub> (TXA<sub>2</sub>)- receptor antagonist. The effect of LPC 20:4 could also be improved by TXA<sub>2</sub>- and PGI<sub>2</sub>-synthase inhibitors. As determined by EIA assays, the tested LPC promoted secretion of PGI<sub>2</sub>, TXA<sub>2</sub>, PGF<sub>2α</sub>, and PGE<sub>2</sub>, however, with markedly different potencies. LPC 16:0 was the most potent inducer of superoxide anion production by mouse aortic rings, followed by LPC 18:2, 20:4 and 18:1, respectively. The strong antioxidant tempol recovered relaxation impairment caused by LPC 18:2, 18:1 and 20:4, but not by LPC 16:0. The tested LPC attenuate ACh-induced relaxation through induction of proconstricting prostanoids and superoxide anions. The potency of attenuating relaxation and the relative contribution of underlying mechanisms are strongly related to LPC acyl-chain length and degree of saturation.</p> </div>", "links"=>[], "tags"=>["Biochemistry", "lipids", "Lipid mediators", "Molecular cell biology", "Signal transduction", "Signaling cascades", "Phospholipid signaling cascade", "Signaling in cellular processes", "Lipid signaling", "cardiovascular", "Cardiovascular pharmacology", "Vascular biology", "chain-dependent", "lysophosphatidylcholine", "endothelium-dependent"], "article_id"=>710129, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Shailaja P. Rao", "Monika Riederer", "Margarete Lechleitner", "Martin Hermansson", "Gernot Desoye", "Seth Hallström", "Wolfgang F. Graier", "Sasa Frank"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0065155.s001", "https://dx.doi.org/10.1371/journal.pone.0065155.s002", "https://dx.doi.org/10.1371/journal.pone.0065155.s003", "https://dx.doi.org/10.1371/journal.pone.0065155.s004", "https://dx.doi.org/10.1371/journal.pone.0065155.s005", "https://dx.doi.org/10.1371/journal.pone.0065155.s006"], "stats"=>{"downloads"=>4, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Acyl_Chain_Dependent_Effect_of_Lysophosphatidylcholine_on_Endothelium_Dependent_Vasorelaxation_/710129", "title"=>"Acyl Chain-Dependent Effect of Lysophosphatidylcholine on Endothelium-Dependent Vasorelaxation", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-05-31 00:02:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/1072334"], "description"=>"<p>A) The rings were equilibrated in 100 µl PSS buffer containing 10 µM DETCA and 10 µM lucigenin at 37°C for 30 minutes, followed by addition of PSS (control) or LPC (10 µM). Emitted light (RLU) was recorded every 10 seconds for 30 seconds. The RLU were normalised to protein content of respective aortic rings and expressed as percentage of control set to 100%. <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0065155#s3\" target=\"_blank\">Results</a> are means ± SEM of three separate experiments, each performed with three rings for each LPC. The rings were preincubated without (no LPC) or with 10 µM LPC 18:1 (B), 18:2 (C), 20:4 (D) or 16:0 (E) in the absence or presence of 200 µM Tempol for 30 minutes, followed by precontraction with NE and cumulative addition of ACh. <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0065155#s3\" target=\"_blank\">Results</a> for each condition are mean ± SEM of 12 rings from 6 mice. *P<0.05, **P<0.01.</p>", "links"=>[], "tags"=>["Biochemistry", "lipids", "Lipid mediators", "Molecular cell biology", "Signal transduction", "Signaling cascades", "Phospholipid signaling cascade", "Signaling in cellular processes", "Lipid signaling", "cardiovascular", "Cardiovascular pharmacology", "Vascular biology", "induced", "lpc", "lpc-induced", "impairment"], "article_id"=>710127, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Shailaja P. Rao", "Monika Riederer", "Margarete Lechleitner", "Martin Hermansson", "Gernot Desoye", "Seth Hallström", "Wolfgang F. Graier", "Sasa Frank"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0065155.g006", "stats"=>{"downloads"=>1, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ROS_are_induced_by_LPC_and_contribute_to_LPC_induced_impairment_of_relaxation_/710127", "title"=>"ROS are induced by LPC and contribute to LPC-induced impairment of relaxation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-05-31 00:02:07"}
  • {"files"=>["https://ndownloader.figshare.com/files/1072333"], "description"=>"<p>The rings were incubated in 200 µl aerated PSS under cell culture conditions at 37°C for 1 h. Thereafter, buffer was replaced with fresh PSS supplemented with PSS (control) or 10 µM LPC followed by further incubation under cell culture conditions at 37°C for 1 h. (A) 6-keto PGF<sub>1α</sub>, a stable degradation product of PGI<sub>2</sub> (B), TXB<sub>2</sub>, a stable degradation product of TXA<sub>2</sub>, (C) PGE<sub>2</sub> and (D) PGF<sub>2α</sub> were quantified by EIA assays and rings were solubilized for protein quantification. <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0065155#s3\" target=\"_blank\">Results</a> shown in A and B are means ± SD of four experiments and those in C and D of three experiments, done in triplicates. *P<0.05, **P<0.01***P<0.001.</p>", "links"=>[], "tags"=>["Biochemistry", "lipids", "Lipid mediators", "Molecular cell biology", "Signal transduction", "Signaling cascades", "Phospholipid signaling cascade", "Signaling in cellular processes", "Lipid signaling", "cardiovascular", "Cardiovascular pharmacology", "Vascular biology", "lpc-treated", "aortic"], "article_id"=>710126, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Shailaja P. Rao", "Monika Riederer", "Margarete Lechleitner", "Martin Hermansson", "Gernot Desoye", "Seth Hallström", "Wolfgang F. Graier", "Sasa Frank"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0065155.g005", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Prostanoid_release_from_LPC_treated_aortic_rings_/710126", "title"=>"Prostanoid release from LPC-treated aortic rings.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-05-31 00:02:06"}
  • {"files"=>["https://ndownloader.figshare.com/files/1072328"], "description"=>"<p>The rings were preincubated without (no LPC) or with 10 µM of indicated LPC in the absence or presence of (A,C,D) indomethacin, a non-selective COX inhibitor (20 µM) or (B) CAY10441, a IP receptor antagonist (1 µM) for 30 minutes, followed by precontraction with NE and cumulative addition of ACh. Relaxation values were expressed as a percentage of the NE-induced contraction. Indomethacin improved relaxation attenuated by LPC 18:2 (C) and 20:4 (D). Relaxation attenuated by LPC 16:0 was exaggerated by indomethacin and CAY10441. <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0065155#s3\" target=\"_blank\">Results</a> are mean ± SEM of 20 rings for each case from 10 mice (A), 8 rings for each case from 3 mice (B) and 12 rings for each case from 6 mice (C,D).</p>", "links"=>[], "tags"=>["Biochemistry", "lipids", "Lipid mediators", "Molecular cell biology", "Signal transduction", "Signaling cascades", "Phospholipid signaling cascade", "Signaling in cellular processes", "Lipid signaling", "cardiovascular", "Cardiovascular pharmacology", "Vascular biology", "cox-inhibition", "prostacyclin-action", "lpc-induced", "attenuation"], "article_id"=>710122, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Shailaja P. Rao", "Monika Riederer", "Margarete Lechleitner", "Martin Hermansson", "Gernot Desoye", "Seth Hallström", "Wolfgang F. Graier", "Sasa Frank"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0065155.g002", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Impact_of_COX_inhibition_and_prostacyclin_action_on_LPC_induced_attenuation_of_relaxation_/710122", "title"=>"Impact of COX-inhibition and prostacyclin-action on LPC-induced attenuation of relaxation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-05-31 00:02:02"}

PMC Usage Stats | Further Information

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Relative Metric

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