Brassinosteroids-Induced Systemic Stress Tolerance was Associated with Increased Transcripts of Several Defence-Related Genes in the Phloem in Cucumis sativus
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{"title"=>"Brassinosteroids-Induced Systemic Stress Tolerance was Associated with Increased Transcripts of Several Defence-Related Genes in the Phloem in Cucumis sativus", "type"=>"journal", "authors"=>[{"first_name"=>"Pingfang", "last_name"=>"Li", "scopus_author_id"=>"54420452800"}, {"first_name"=>"Li", "last_name"=>"Chen", "scopus_author_id"=>"57022341500"}, {"first_name"=>"Yanhong", "last_name"=>"Zhou", "scopus_author_id"=>"35477536400"}, {"first_name"=>"Xiaojian", "last_name"=>"Xia", "scopus_author_id"=>"23568883200"}, {"first_name"=>"Kai", "last_name"=>"Shi", "scopus_author_id"=>"13807542600"}, {"first_name"=>"Zhixiang", "last_name"=>"Chen", "scopus_author_id"=>"7409485867"}, {"first_name"=>"Jingquan", "last_name"=>"Yu", "scopus_author_id"=>"55584057900"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84879206294", "sgr"=>"84879206294", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0066582", "pmid"=>"23840504", "isbn"=>"1932-6203", "pui"=>"369155375"}, "id"=>"50bb03bf-2112-36f8-a6cf-081741e52137", "abstract"=>"Brassinosteroids (BRs), a group of naturally occurring plant steroidal compounds, are essential for plant growth, development and stress tolerance. Recent studies showed that BRs could induce systemic tolerance to biotic and abiotic stresses; however, the molecular mechanisms by which BRs signals lead to responses in the whole plant are largely unknown. In this study, 24-epibrassinosteroid (EBR)-induced systemic tolerance in Cucumis sativus L. cv. Jinyan No. 4 was analyzed through the assessment of symptoms of photooxidative stress by chlorophyll fluorescence imaging pulse amplitude modulation. Expression of defense/stress related genes were induced in both treated local leaves and untreated systemic leaves by local EBR application. With the suppressive subtractive hybridization (SSH) library using cDNA from the phloem sap of EBR-treated plants as the tester and distilled water (DW)-treated plants as the driver, 14 transcripts out of 260 clones were identified. Quantitative Real Time-Polymerase Chain Reaction (RT-qPCR) validated the specific up-regulation of these transcripts. Of the differentially expressed transcripts with known functions, transcripts for the selected four cDNAs, which encode an auxin-responsive protein (IAA14), a putative ankyrin-repeat protein, an F-box protein (PP2), and a major latex, pathogenesis-related (MLP)-like protein, were induced in local leaves, systemic leaves and roots after foliar application of EBR onto mature leaves. Our results demonstrated that EBR-induced systemic tolerance is accompanied with increased transcript of genes in the defense response in other organs. The potential role of phloem mRNAs as signaling components in mediating BR-regulated systemic resistance is discussed.", "link"=>"http://www.mendeley.com/research/brassinosteroidsinduced-systemic-stress-tolerance-associated-increased-transcripts-several-defencere", "reader_count"=>24, "reader_count_by_academic_status"=>{"Researcher"=>9, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>1, "Professor"=>1, "Unspecified"=>1}, "reader_count_by_user_role"=>{"Researcher"=>9, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>1, "Professor"=>1, "Unspecified"=>1}, "reader_count_by_subject_area"=>{"Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>1, "Agricultural and Biological Sciences"=>21, "Unspecified"=>1}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>21}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Environmental Science"=>{"Environmental Science"=>1}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"United States"=>1, "Japan"=>1, "Ukraine"=>1, "Spain"=>1}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1093471"], "description"=>"<p>(A) The 5<sup>th</sup> leaf of cucumber seedlings corresponding to the local treated leaf at the seven-leaf stage was pretreated with distilled water (DW) or 0.2 µM EBR; 24 h later the upper leaf (6<sup>th</sup>) corresponding to the systemic leaf was exposed to 10 µM paraquat (PQ), photographs were taken at 24 h after PQ treatment. (B) The maximal photochemical quantum efficiency of photosystem II (Fv/Fm) was monitored at 24 h after PQ treatment with imaging pulse amplitude modulation (PAM) to assess the changes in tolerance. (C) Fv/Fm measurement of systemic leaves. Data represent the means of five replicates ± SD. Bars sharing the same letters are not significantly different within the treatment (<i>P</i>≤0.05).</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Defense proteins", "developmental biology", "Plant growth and development", "Molecular cell biology", "Cellular stress responses", "Plant science", "Agronomy", "Horticulture", "Plant cell biology", "phloem", "Plant physiology", "induced", "systemic", "cucumber"], "article_id"=>725495, "categories"=>["Biological Sciences"], "users"=>["Pingfang Li", "Li Chen", "Yanhong Zhou", "Xiaojian Xia", "Kai Shi", "Zhixiang Chen", "Jingquan Yu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0066582.g001", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_EBR_induced_systemic_stress_tolerance_in_cucumber_plants_/725495", "title"=>"EBR induced systemic stress tolerance in cucumber plants.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-06-19 01:31:35"}
  • {"files"=>["https://ndownloader.figshare.com/files/1093473"], "description"=>"<p>The 5<sup>th</sup> leaf of cucumber seedlings at the seven-leaf stage was pretreated with distilled water (DW) or 0.2 µM EBR, the 5<sup>th</sup> leaf corresponding to the local treated leaf and the 6<sup>th</sup> leaf corresponding to the systemic leaf were harvested at 3, 6, 12, 24 h after EBR treatment. Relative mRNA abundances of <i>cAPX</i>, <i>CAT</i>, <i>MDAR</i>, <i>GR</i> in EBR-treated local and untreated systemic leaves were determined by RT-qPCR analysis. Data represent the means of three replicates ± SD. Asterisks (*) indicate a significant difference from the untreated control at <i>P</i>≤0.05 according to Tukey’s test.</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Defense proteins", "developmental biology", "Plant growth and development", "Molecular cell biology", "Cellular stress responses", "Plant science", "Agronomy", "Horticulture", "Plant cell biology", "phloem", "Plant physiology", "genes", "systemic", "cucumber"], "article_id"=>725497, "categories"=>["Biological Sciences"], "users"=>["Pingfang Li", "Li Chen", "Yanhong Zhou", "Xiaojian Xia", "Kai Shi", "Zhixiang Chen", "Jingquan Yu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0066582.g002", "stats"=>{"downloads"=>2, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_analysis_of_defense_stress_related_genes_in_local_and_systemic_cucumber_leaves_/725497", "title"=>"Expression analysis of defense/stress related genes in local and systemic cucumber leaves.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-06-19 01:31:37"}
  • {"files"=>["https://ndownloader.figshare.com/files/1093474"], "description"=>"<p>Phloem sap was collected from stem cut close to apex and at petioles during various time intervals (3, 6, 9 and 24 h) after foliar EBR treatment. RNA was extracted from the pooled samples and relative mRNA abundances of transcripts obtained from the SSH library were determined by RT-qPCR. The data were obtained from three separate replicates, each value in the graph shows means ± SD. Asterisks (*) indicate a significant difference from the untreated control at <i>P</i>≤0.05 according to Tukey’s test.</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Defense proteins", "developmental biology", "Plant growth and development", "Molecular cell biology", "Cellular stress responses", "Plant science", "Agronomy", "Horticulture", "Plant cell biology", "phloem", "Plant physiology", "up-regulated", "transcripts", "ssh"], "article_id"=>725498, "categories"=>["Biological Sciences"], "users"=>["Pingfang Li", "Li Chen", "Yanhong Zhou", "Xiaojian Xia", "Kai Shi", "Zhixiang Chen", "Jingquan Yu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0066582.g003", "stats"=>{"downloads"=>2, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Validation_of_the_up_regulated_transcripts_present_in_the_SSH_library_by_using_RT_qPCR_/725498", "title"=>"Validation of the up-regulated transcripts present in the SSH library by using RT-qPCR.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-06-19 01:31:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1093475"], "description"=>"<p>The 5<sup>th</sup> leaf of cucumber seedlings at the seven-leaf stage was pretreated with distilled water (DW) or 0.2 µM EBR, the 5<sup>th</sup> leaf corresponding to the local treated leaf and the 6<sup>th</sup> leaf corresponding to the systemic leaf were harvested at 0.5, 1, 3 and 6 h after EBR treatment. Relative mRNA abundances of Cs453, Cs579, Cs594 and Cs623 obtained from the SSH library were determined in local and systemic leaves by RT-qPCR analysis. The data were obtained from three separate replicates, and each value in the graph shows means ± SD. Asterisks (*) indicate a significant difference from the untreated control at <i>P</i>≤0.05 according to Tukey’s test.</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Defense proteins", "developmental biology", "Plant growth and development", "Molecular cell biology", "Cellular stress responses", "Plant science", "Agronomy", "Horticulture", "Plant cell biology", "phloem", "Plant physiology", "transcripts", "ssh", "cucumber"], "article_id"=>725499, "categories"=>["Biological Sciences"], "users"=>["Pingfang Li", "Li Chen", "Yanhong Zhou", "Xiaojian Xia", "Kai Shi", "Zhixiang Chen", "Jingquan Yu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0066582.g004", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_analysis_of_defense_related_transcripts_obtained_from_SSH_library_in_cucumber_leaves_/725499", "title"=>"Expression analysis of defense related transcripts obtained from SSH library in cucumber leaves.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-06-19 01:31:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/1093477"], "description"=>"<p>After EBR (0.2 µM) sprayed on mature leaves, roots were harvested at 6, 12, and 24 h after treatment. Relative mRNA abundances of Cs453, Cs579, Cs594 and Cs623 obtained from the SSH library were determined by RT-qPCR analysis. The data were obtained from three separate replicates, and each value in the graph shows means ± SD. Asterisks (*) indicate a significant difference from the untreated control at <i>P</i>≤0.05 according to Tukey’s test.</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Defense proteins", "developmental biology", "Plant growth and development", "Molecular cell biology", "Cellular stress responses", "Plant science", "Agronomy", "Horticulture", "Plant cell biology", "phloem", "Plant physiology", "transcripts", "ssh", "cucumber"], "article_id"=>725501, "categories"=>["Biological Sciences"], "users"=>["Pingfang Li", "Li Chen", "Yanhong Zhou", "Xiaojian Xia", "Kai Shi", "Zhixiang Chen", "Jingquan Yu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0066582.g005", "stats"=>{"downloads"=>2, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_analysis_of_defense_related_transcripts_obtained_from_SSH_library_in_cucumber_roots_/725501", "title"=>"Expression analysis of defense related transcripts obtained from SSH library in cucumber roots.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-06-19 01:31:41"}
  • {"files"=>["https://ndownloader.figshare.com/files/1093478"], "description"=>"<p>EBR-induced changes in the population of mRNAs in the phloem sap of cucumber plants were determined by suppressive subtractive hybridization (SSH) methods. Mature leaves of seven-leaf stage plants were treated with distilled water (DW) or 0.2 µM EBR and phloem sap was collected from stem cut close to apex and at petioles during various time intervals (3, 6, 9 and 24 h) after treatment. RNA was extracted from pooled samples for use in SSH analysis. Data presented reflects the mRNA species up-regulated in EBR-treated vs DW-treated phloem sap.</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Defense proteins", "developmental biology", "Plant growth and development", "Molecular cell biology", "Cellular stress responses", "Plant science", "Agronomy", "Horticulture", "Plant cell biology", "phloem", "Plant physiology", "mrna", "transcripts", "induced", "24-epibrassinosteroid", "sap", "cucumber"], "article_id"=>725502, "categories"=>["Biological Sciences"], "users"=>["Pingfang Li", "Li Chen", "Yanhong Zhou", "Xiaojian Xia", "Kai Shi", "Zhixiang Chen", "Jingquan Yu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0066582.t001", "stats"=>{"downloads"=>9, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Differential_population_of_the_mRNA_transcripts_induced_by_24_epibrassinosteroid_EBR_in_phloem_sap_of_cucumber_plants_/725502", "title"=>"Differential population of the mRNA transcripts induced by 24-epibrassinosteroid (EBR) in phloem sap of cucumber plants.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-06-19 01:31:42"}
  • {"files"=>["https://ndownloader.figshare.com/files/1093479"], "description"=>"<div><p>Brassinosteroids (BRs), a group of naturally occurring plant steroidal compounds, are essential for plant growth, development and stress tolerance. Recent studies showed that BRs could induce systemic tolerance to biotic and abiotic stresses; however, the molecular mechanisms by which BRs signals lead to responses in the whole plant are largely unknown. In this study, 24-epibrassinosteroid (EBR)-induced systemic tolerance in <i>Cucumis sativus</i> L. cv. Jinyan No. 4 was analyzed through the assessment of symptoms of photooxidative stress by chlorophyll fluorescence imaging pulse amplitude modulation. Expression of defense/stress related genes were induced in both treated local leaves and untreated systemic leaves by local EBR application. With the suppressive subtractive hybridization (SSH) library using cDNA from the phloem sap of EBR-treated plants as the tester and distilled water (DW)-treated plants as the driver, 14 transcripts out of 260 clones were identified. Quantitative Real Time-Polymerase Chain Reaction (RT-qPCR) validated the specific up-regulation of these transcripts. Of the differentially expressed transcripts with known functions, transcripts for the selected four cDNAs, which encode an auxin-responsive protein (IAA14), a putative ankyrin-repeat protein, an F-box protein (PP2), and a major latex, pathogenesis-related (MLP)-like protein, were induced in local leaves, systemic leaves and roots after foliar application of EBR onto mature leaves. Our results demonstrated that EBR-induced systemic tolerance is accompanied with increased transcript of genes in the defense response in other organs. The potential role of phloem mRNAs as signaling components in mediating BR-regulated systemic resistance is discussed.</p></div>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Defense proteins", "developmental biology", "Plant growth and development", "Molecular cell biology", "Cellular stress responses", "Plant science", "Agronomy", "Horticulture", "Plant cell biology", "phloem", "Plant physiology", "brassinosteroids-induced", "systemic", "transcripts", "defence-related", "genes"], "article_id"=>725503, "categories"=>["Biological Sciences"], "users"=>["Pingfang Li", "Li Chen", "Yanhong Zhou", "Xiaojian Xia", "Kai Shi", "Zhixiang Chen", "Jingquan Yu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0066582", "stats"=>{"downloads"=>9, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Brassinosteroids_Induced_Systemic_Stress_Tolerance_was_Associated_with_Increased_Transcripts_of_Several_Defence_Related_Genes_in_the_Phloem_in_Cucumis_sativus_/725503", "title"=>"Brassinosteroids-Induced Systemic Stress Tolerance was Associated with Increased Transcripts of Several Defence-Related Genes in the Phloem in <i>Cucumis sativus</i>", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-06-19 01:31:43"}

PMC Usage Stats | Further Information

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Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Agriculture", "average_usage"=>[241, 422, 551, 659, 765, 866, 954, 1043, 1142, 1235, 1311, 1397, 1462]}, {"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[266, 468, 593, 703, 804, 903, 993, 1084, 1171, 1256, 1339, 1422, 1492]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[284, 491, 620, 738, 843, 945, 1043, 1137, 1225, 1315, 1400, 1479, 1555]}, {"subject_area"=>"/Biology and life sciences/Organisms", "average_usage"=>[281, 484, 611, 728, 835, 934, 1030, 1123, 1214, 1299, 1383, 1464]}]}
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