Season, Irrigation, Leaf Age, and Escherichia coli Inoculation Influence the Bacterial Diversity in the Lettuce Phyllosphere
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Season, Irrigation, Leaf Age, and Escherichia coli Inoculation Influence the Bacterial Diversity in the Lettuce Phyllosphere", "type"=>"journal", "authors"=>[{"first_name"=>"Thomas R.", "last_name"=>"Williams", "scopus_author_id"=>"56052793900"}, {"first_name"=>"Anne Laure", "last_name"=>"Moyne", "scopus_author_id"=>"6507612119"}, {"first_name"=>"Linda J.", "last_name"=>"Harris", "scopus_author_id"=>"7401536706"}, {"first_name"=>"Maria L.", "last_name"=>"Marco", "scopus_author_id"=>"15827824100"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84879743032", "doi"=>"10.1371/journal.pone.0068642", "pui"=>"369253084", "pmid"=>"23844230", "scopus"=>"2-s2.0-84879743032", "issn"=>"19326203", "isbn"=>"1932-6203"}, "id"=>"c48b5597-ba86-3620-9479-8755b223da4d", "abstract"=>"The developmental and temporal succession patterns and disturbance responses of phyllosphere bacterial communities are largely unknown. These factors might influence the capacity of human pathogens to persist in association with those communities on agriculturally-relevant plants. In this study, the phyllosphere microbiota was identified for Romaine lettuce plants grown in the Salinas Valley, CA, USA from four plantings performed over 2 years and including two irrigation methods and inoculations with an attenuated strain of Escherichia coli O157:H7. High-throughput DNA pyrosequencing of the V5 to V9 variable regions of bacterial 16S rRNA genes recovered in lettuce leaf washes revealed that the bacterial diversity in the phyllosphere was distinct for each field trial but was also strongly correlated with the season of planting. Firmicutes were generally most abundant in early season (June) plantings and Proteobacteria comprised the majority of bacteria recovered later in the year (August and October). Comparisons within individual field trials showed that bacterial diversity differed between sprinkler (overhead) and drip (surface) irrigated lettuce and increased over time as the plants grew. The microbiota were also distinct between control and E. coli O157:H7-inoculated plants and between E. coli O157:H7-inoculated plants with and without surviving pathogen cells. The bacterial inhabitants of the phyllosphere therefore appear to be affected by seasonal, irrigation, and biological factors in ways that are relevant for assessments of fresh produce food safety.", "link"=>"http://www.mendeley.com/research/season-irrigation-leaf-age-escherichia-coli-inoculation-influence-bacterial-diversity-lettuce-phyllo", "reader_count"=>79, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>3, "Researcher"=>18, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>22, "Student > Postgraduate"=>3, "Student > Master"=>16, "Other"=>2, "Student > Bachelor"=>6, "Lecturer"=>1, "Professor"=>3}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>3, "Researcher"=>18, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>22, "Student > Postgraduate"=>3, "Student > Master"=>16, "Other"=>2, "Student > Bachelor"=>6, "Lecturer"=>1, "Professor"=>3}, "reader_count_by_subject_area"=>{"Unspecified"=>7, "Environmental Science"=>5, "Biochemistry, Genetics and Molecular Biology"=>3, "Mathematics"=>1, "Agricultural and Biological Sciences"=>55, "Medicine and Dentistry"=>1, "Business, Management and Accounting"=>1, "Chemical Engineering"=>1, "Chemistry"=>1, "Immunology and Microbiology"=>3, "Economics, Econometrics and Finance"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>3}, "Economics, Econometrics and Finance"=>{"Economics, Econometrics and Finance"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>55}, "Business, Management and Accounting"=>{"Business, Management and Accounting"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Mathematics"=>{"Mathematics"=>1}, "Unspecified"=>{"Unspecified"=>7}, "Environmental Science"=>{"Environmental Science"=>5}, "Chemical Engineering"=>{"Chemical Engineering"=>1}}, "reader_count_by_country"=>{"Armenia"=>1, "Canada"=>1, "United States"=>5, "France"=>1, "Spain"=>1}, "group_count"=>3}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84879743032"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84879743032?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84879743032&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84879743032&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84879743032", "dc:identifier"=>"SCOPUS_ID:84879743032", "eid"=>"2-s2.0-84879743032", "dc:title"=>"Season, Irrigation, Leaf Age, and Escherichia coli Inoculation Influence the Bacterial Diversity in the Lettuce Phyllosphere", "dc:creator"=>"Williams T.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"8", "prism:issueIdentifier"=>"7", "prism:pageRange"=>nil, "prism:coverDate"=>"2013-07-02", "prism:coverDisplayDate"=>"2 July 2013", "prism:doi"=>"10.1371/journal.pone.0068642", "citedby-count"=>"66", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"University of California, Davis", "affiliation-city"=>"Davis", "affiliation-country"=>"United States"}], "pubmed-id"=>"23844230", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e68642", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0068642", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"66", "xml_views"=>"12", "html_views"=>"429"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"31", "xml_views"=>"1", "html_views"=>"118"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"121"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"18", "xml_views"=>"2", "html_views"=>"99"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"2", "html_views"=>"163"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"182"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"25", "xml_views"=>"0", "html_views"=>"98"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"98"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"15", "xml_views"=>"2", "html_views"=>"143"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"24", "xml_views"=>"1", "html_views"=>"139"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"110"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"69"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"2", "html_views"=>"58"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"2", "html_views"=>"55"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"63"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"64"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"44"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"32", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"43"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"66"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"35"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"51"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"37"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"43"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"21", "xml_views"=>"2", "html_views"=>"51"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"93"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"39"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"17", "xml_views"=>"2", "html_views"=>"20"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"5", "html_views"=>"14"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"23", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"22", "xml_views"=>"5", "html_views"=>"16"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"20", "xml_views"=>"4", "html_views"=>"24"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"23", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"3", "year"=>"2020", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"4", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2020", "pdf_views"=>"29", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"6", "year"=>"2020", "pdf_views"=>"10", "xml_views"=>"3", "html_views"=>"11"}
  • {"month"=>"7", "year"=>"2020", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"16"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1108979"], "description"=>"<p>The total aerobic bacterial density enumerated on TSA for inoculated plants with viable E. coli O157:H7 (InocPOS) and those without (InocNEG) averaged for all inoculated plants from 2 to 28 dpi within a field trial. The 0 and 2 hpi time points were omitted due to the presence of large abundance of the E. coli O157:H7; 7 dpi plants were omitted from the E09 trial because enrichment results were not available. An * indicates that significantly more bacteria were found on InocPOS or InocNEG plants by Student’s t-test; P ≤ 0.001. </p>", "links"=>[], "tags"=>["culturable", "aerobic", "bacterial", "abundances", "plants", "inocpos", "inocneg"], "article_id"=>737738, "categories"=>["Biological Sciences"], "users"=>["Thomas R. Williams", "Anne-Laure Moyne", "Linda J. Harris", "Maria L. Marco"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0068642.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Total_culturable_aerobic_bacterial_abundances_on_plants_that_were_InocPOS_or_InocNEG_for_E_coli_O157_H7_/737738", "title"=>"Total culturable aerobic bacterial abundances on plants that were InocPOS or InocNEG for <i>E. coli</i> O157:H7.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-02 03:39:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/1108980"], "description"=>"<p>16S rRNA sequences obtained by pyrosequencing were quality-filtered and assigned a phylum (A) and family (B) classification. Average relative abundances are shown for each field trial.</p>", "links"=>[], "tags"=>["seasonal", "variability", "romaine", "lettuce"], "article_id"=>737739, "categories"=>["Biological Sciences"], "users"=>["Thomas R. Williams", "Anne-Laure Moyne", "Linda J. Harris", "Maria L. Marco"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0068642.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Microbial_diversity_and_seasonal_variability_in_the_Romaine_lettuce_phyllosphere_/737739", "title"=>"Microbial diversity and seasonal variability in the Romaine lettuce phyllosphere.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-02 03:39:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/1108981"], "description"=>"<p>Each bar represents the average and standard error of the weighted-UniFrac distances of pairwise comparisons for each set of samples in the respective subset of data. Black bars represent the average distance between field trials whereas the gray bars are the average distances between samples within trials (* <i>P</i> ≤ 0.001 by the Student’s t-test).</p>", "links"=>[], "tags"=>["phyllosphere"], "article_id"=>737740, "categories"=>["Biological Sciences"], "users"=>["Thomas R. Williams", "Anne-Laure Moyne", "Linda J. Harris", "Maria L. Marco"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0068642.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Lettuce_phyllosphere_community_structure_between_and_within_field_trials_/737740", "title"=>"Lettuce phyllosphere community structure between and within field trials.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-02 03:39:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/1108982"], "description"=>"<p>The UPGMA dendrogram was made in QIIME using weighted UniFrac distance data between phyllosphere microbiota from 223 plants. Tree branches in blue correspond to plants from the E09 and E10 trials and red branches represent samples from the L09 and L10 trials. Clusters I and II were identified as two major sample groups. Relative sequence abundances of the top 21 taxonomic groups are shown in the order of sequence abundance. The heat map indicates a range of proportional sequence abundance in each sample (minimum: 0%; maximum: 87%).</p>", "links"=>[], "tags"=>["phyllosphere", "differs"], "article_id"=>737741, "categories"=>["Biological Sciences"], "users"=>["Thomas R. Williams", "Anne-Laure Moyne", "Linda J. Harris", "Maria L. Marco"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0068642.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Bacterial_diversity_phyllosphere_differs_between_season_of_planting_/737741", "title"=>"Bacterial diversity phyllosphere differs between season of planting.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-02 03:39:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/1108983"], "description"=>"<p>The Phylogenetic Diversity (PD) of the phyllosphere microbiota from leaves of different ages (dpi). The average ± standard error for all plants the four field trials is shown. n= 73, 84, and 66 for 7, 14, and 21 dpi, respectively; * <i>P</i> ≤ 0.001 by the Kruskal-Wallis multiple comparisons test. ns: not significant.</p>", "links"=>[], "tags"=>["influences", "phyllosphere", "microbial"], "article_id"=>737742, "categories"=>["Biological Sciences"], "users"=>["Thomas R. Williams", "Anne-Laure Moyne", "Linda J. Harris", "Maria L. Marco"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0068642.g006"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Leaf_age_influences_the_diversity_of_phyllosphere_microbial_diversity_/737742", "title"=>"Leaf age influences the diversity of phyllosphere microbial diversity.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-02 03:39:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/1108984"], "description"=>"<p>Phyllosphere microbiota identified during the L10 were analyzed by Principal Coordinates Analysis (PCoA) performed on Unifrac distances between bacterial communities collected from plants on different days (A), and for plants at 14 dpi exposed to drip or overhead irrigation (B), the inoculation of <i>E. coli</i> O157:H7 (C), or inoculated plants that were InocPOS or InocNEG (D). Weighted UniFrac distances were used for (A), (C), and (D) and unweighted UniFrac distances were used for (B). The plots are representative of findings for the other field trials and time points. Additional PCoA plots are in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0068642#pone.0068642.s007\" target=\"_blank\">Figure S7</a>.</p>", "links"=>[], "tags"=>["microbiota", "differs", "inoculation"], "article_id"=>737743, "categories"=>["Biological Sciences"], "users"=>["Thomas R. Williams", "Anne-Laure Moyne", "Linda J. Harris", "Maria L. Marco"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0068642.g007"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Phyllosphere_microbiota_differs_due_to_effects_of_the_day_of_collection_irrigation_and_inoculation_of_E_coli_O157_H7_/737743", "title"=>"Phyllosphere microbiota differs due to effects of the day of collection, irrigation, and inoculation of <i>E. coli</i> O157:H7.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-02 03:39:40"}
  • {"files"=>["https://ndownloader.figshare.com/files/1108990", "https://ndownloader.figshare.com/files/1108991", "https://ndownloader.figshare.com/files/1108992", "https://ndownloader.figshare.com/files/1108993", "https://ndownloader.figshare.com/files/1108995", "https://ndownloader.figshare.com/files/1108996", "https://ndownloader.figshare.com/files/1108997", "https://ndownloader.figshare.com/files/1108998", "https://ndownloader.figshare.com/files/1108999", "https://ndownloader.figshare.com/files/1109000"], "description"=>"<div><p>The developmental and temporal succession patterns and disturbance responses of phyllosphere bacterial communities are largely unknown. These factors might influence the capacity of human pathogens to persist in association with those communities on agriculturally-relevant plants. In this study, the phyllosphere microbiota was identified for Romaine lettuce plants grown in the Salinas Valley, CA, USA from four plantings performed over 2 years and including two irrigation methods and inoculations with an attenuated strain of <i>Escherichia coli</i> O157:H7. High-throughput DNA pyrosequencing of the V5 to V9 variable regions of bacterial 16S rRNA genes recovered in lettuce leaf washes revealed that the bacterial diversity in the phyllosphere was distinct for each field trial but was also strongly correlated with the season of planting. Firmicutes were generally most abundant in early season (June) plantings and Proteobacteria comprised the majority of bacteria recovered later in the year (August and October). Comparisons within individual field trials showed that bacterial diversity differed between sprinkler (overhead) and drip (surface) irrigated lettuce and increased over time as the plants grew. The microbiota were also distinct between control and <i>E. coli</i> O157:H7-inoculated plants and between <i>E. coli</i> O157:H7-inoculated plants with and without surviving pathogen cells. The bacterial inhabitants of the phyllosphere therefore appear to be affected by seasonal, irrigation, and biological factors in ways that are relevant for assessments of fresh produce food safety.</p> </div>", "links"=>[], "tags"=>["inoculation", "bacterial", "lettuce", "phyllosphere"], "article_id"=>737745, "categories"=>["Biological Sciences"], "users"=>["Thomas R. Williams", "Anne-Laure Moyne", "Linda J. Harris", "Maria L. Marco"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0068642.s001", "https://dx.doi.org/10.1371/journal.pone.0068642.s002", "https://dx.doi.org/10.1371/journal.pone.0068642.s003", "https://dx.doi.org/10.1371/journal.pone.0068642.s004", "https://dx.doi.org/10.1371/journal.pone.0068642.s005", "https://dx.doi.org/10.1371/journal.pone.0068642.s006", "https://dx.doi.org/10.1371/journal.pone.0068642.s007", "https://dx.doi.org/10.1371/journal.pone.0068642.s008", "https://dx.doi.org/10.1371/journal.pone.0068642.s009", "https://dx.doi.org/10.1371/journal.pone.0068642.s010"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Season_Irrigation_Leaf_Age_and_Escherichia_coli_Inoculation_Influence_the_Bacterial_Diversity_in_the_Lettuce_Phyllosphere/737745", "title"=>"Season, Irrigation, Leaf Age, and <i>Escherichia coli</i> Inoculation Influence the Bacterial Diversity in the Lettuce Phyllosphere", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-07-02 03:39:40"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"20", "full-text"=>"14", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"20", "cited-by"=>"0", "year"=>"2013", "month"=>"7"}
  • {"unique-ip"=>"28", "full-text"=>"31", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"49", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"26", "full-text"=>"25", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"14", "pdf"=>"1", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"25", "full-text"=>"29", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"17", "full-text"=>"20", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"13", "full-text"=>"19", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"21", "full-text"=>"20", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"19", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"23", "full-text"=>"25", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"23", "full-text"=>"23", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"21", "full-text"=>"22", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"20", "full-text"=>"21", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"15", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"1", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"25", "full-text"=>"20", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"1", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"14", "full-text"=>"16", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"13", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"19", "full-text"=>"17", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"22", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"20", "full-text"=>"14", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"1", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"2"}
  • {"unique-ip"=>"22", "full-text"=>"22", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2020", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2020", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2020", "month"=>"6"}

Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Agriculture", "average_usage"=>[241, 422, 551, 659, 765, 866, 954, 1043, 1142, 1235, 1311, 1397, 1462]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[272, 466, 589, 702, 806, 903, 995, 1086, 1176, 1258, 1347, 1422, 1493]}, {"subject_area"=>"/Biology and life sciences/Organisms", "average_usage"=>[281, 484, 611, 728, 835, 934, 1030, 1123, 1214, 1299, 1383, 1464]}, {"subject_area"=>"/Biology and life sciences/Plant science", "average_usage"=>[269, 451, 577, 699, 812, 922, 1019, 1126, 1225, 1309, 1404, 1493, 1563]}]}
Loading … Spinner
There are currently no alerts