Threonine Affects Intestinal Function, Protein Synthesis and Gene Expression of TOR in Jian Carp (Cyprinus carpio var. Jian)
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{"title"=>"Threonine Affects Intestinal Function, Protein Synthesis and Gene Expression of TOR in Jian Carp (Cyprinus carpio var. Jian)", "type"=>"journal", "authors"=>[{"first_name"=>"Lin", "last_name"=>"Feng", "scopus_author_id"=>"55507811300"}, {"first_name"=>"Yan", "last_name"=>"Peng", "scopus_author_id"=>"57199767792"}, {"first_name"=>"Pei", "last_name"=>"Wu", "scopus_author_id"=>"39062242600"}, {"first_name"=>"Kai", "last_name"=>"Hu", "scopus_author_id"=>"36655852300"}, {"first_name"=>"Wei Dan", "last_name"=>"Jiang", "scopus_author_id"=>"36655984000"}, {"first_name"=>"Yang", "last_name"=>"Liu", "scopus_author_id"=>"56459749900"}, {"first_name"=>"Jun", "last_name"=>"Jiang", "scopus_author_id"=>"56896492000"}, {"first_name"=>"Shu Hong", "last_name"=>"Li", "scopus_author_id"=>"35088377500"}, {"first_name"=>"Xiao Qiu", "last_name"=>"Zhou", "scopus_author_id"=>"56168383500"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"23922879", "sgr"=>"84880837975", "pui"=>"369439173", "scopus"=>"2-s2.0-84880837975", "issn"=>"19326203", "isbn"=>"1932-6203 (Electronic); 1932-6203 (Linking)", "doi"=>"10.1371/journal.pone.0069974"}, "id"=>"1ffd3f0a-b1a8-3bd2-9644-d81adef6ce31", "abstract"=>"This study aimed to investigate the effects of threonine (Thr) on the digestive and absorptive ability, proliferation and differentiation of enterocytes, and gene expression of juvenile Jian carp (Cyprinus carpio var. Jian). First, seven isonitrogenous diets containing graded levels of Thr (7.4-25.2 g/kg diet) were fed to the fishes for 60 days. Second, enterocyte proliferation and differentiation were assayed by culturing enterocytes with graded levels of Thr (0-275 mg/l) in vitro. Finally, enterocytes were cultured with 0 and 205 mg/l Thr to determine protein synthesis. The percent weight gain (PWG), specific growth rate, feed intake, feed efficiency, protein retention value, activities of trypsin, lipase and amylase, weights and protein contents of hepatopancreas and intestine, folds heights, activities of alkaline phosphatase (AKP), γ- glutamyl transpeptidase and Na(+)/K(+)-ATPase in all intestinal segments, glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT) activities in hepatopancreas, and 4E-BP2 gene expression in muscle, hepatopancreas and intestinal segments were significantly enhanced by Thr (p<0.05). However, the plasma ammonia concentration and TOR gene expression decreased (p<0.05). In vitro, Thr supplement significantly increased cell numbers, protein content, the activities of GOT, GPT, AKP and Na(+)/K(+)-ATPase, and protein synthesis rate of enterocytes, and decreased LDH activity and ammonia content in cell medium (p<0.05). In conclusion, Thr improved growth, digestive and absorptive capacity, enterocyte proliferation and differentiation, and protein synthesis and regulated TOR and 4E-BP2 gene expression in juvenile Jian carp. The dietary Thr requirement of juvenile Jian carp was 16.25 g/kg diet (51.3 g/kg protein) based on quadratic regression analysis of PWG.", "link"=>"http://www.mendeley.com/research/threonine-affects-intestinal-function-protein-synthesis-gene-expression-tor-jian-carp-cyprinus-carpi", "reader_count"=>16, "reader_count_by_academic_status"=>{"Student > Doctoral Student"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>5, "Student > Master"=>3, "Student > Bachelor"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>2}, "reader_count_by_user_role"=>{"Student > Doctoral Student"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>5, "Student > Master"=>3, "Student > Bachelor"=>1, "Lecturer > Senior Lecturer"=>1, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Agricultural and Biological Sciences"=>9, "Business, Management and Accounting"=>1, "Veterinary Science and Veterinary Medicine"=>2, "Chemistry"=>1, "Computer Science"=>1, "Earth and Planetary Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Chemistry"=>{"Chemistry"=>1}, "Earth and Planetary Sciences"=>{"Earth and Planetary Sciences"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>9}, "Computer Science"=>{"Computer Science"=>1}, "Business, Management and Accounting"=>{"Business, Management and Accounting"=>1}, "Unspecified"=>{"Unspecified"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>2}}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1133011"], "description"=>"1<p>Values are means ± SD, means of three replicates with fifty fish per replicate. Mean values with the different superscripts in the same row are significantly different (<i>p</i><0.05).</p>2<p>Values are means ± SD, n = 5. Mean values with the different superscripts in the same row are significantly different (<i>p</i><0.05). IBW, Initial body weight; FBW, final body weight; PWG, percent weight gain; SGR, specific growth rate; FI, feed intake; FE, feed efficiency; PER, protein efficiency ratio; CP, crude protein; PRV, protein retention value.</p>", "links"=>[], "tags"=>["Animal management", "Animal nutrition", "aquaculture", "Fish farming", "biophysics", "Molecular cell biology", "cell division", "Cell growth", "gene expression", "Signal transduction", "Natural materials", "juvenile", "jian", "carp", "fed", "diets", "containing", "graded"], "article_id"=>756603, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Lin Feng", "Yan Peng", "Pei Wu", "Kai Hu", "Wei-Dan Jiang", "Yang Liu", "Jun Jiang", "Shu-Hong Li", "Xiao-Qiu Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0069974.t001", "stats"=>{"downloads"=>1, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Growth_performance_final_body_composition_and_survival_of_juvenile_Jian_carp_fed_diets_containing_graded_levels_of_Thr_/756603", "title"=>"Growth performance, final body composition and survival of juvenile Jian carp fed diets containing graded levels of Thr.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-07-26 02:17:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1133010"], "description"=>"1<p>Values are means ± SD, n = 15. Mean values with the different superscripts in the same row are significantly different (<i>p</i><0.05).</p>2<p>Values are means ± SD, n = 5. Mean values with the different superscripts in the same row are significantly different (<i>p</i><0.05). HW, hepatopancreas weight; HSI, hepatosomatic index; HPC, hepatopancreas protein content; IW, intestine weight; IL, intestine length; RGL, relative gut length; ISI, intestosomatic index; IPC, intestine protein content; PI, proximal intestine; MI, mid intestine; DI, distal intestine.</p>", "links"=>[], "tags"=>["Animal management", "Animal nutrition", "aquaculture", "Fish farming", "biophysics", "Molecular cell biology", "cell division", "Cell growth", "gene expression", "Signal transduction", "Natural materials", "hepatopancreas", "intestinal", "folds", "juvenile", "jian", "carp", "fed", "diets", "containing", "graded"], "article_id"=>756602, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Lin Feng", "Yan Peng", "Pei Wu", "Kai Hu", "Wei-Dan Jiang", "Yang Liu", "Jun Jiang", "Shu-Hong Li", "Xiao-Qiu Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0069974.t002", "stats"=>{"downloads"=>42, "page_views"=>37, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Growth_and_development_of_hepatopancreas_and_intestine_and_intestinal_folds_height_of_juvenile_Jian_carp_fed_diets_containing_graded_levels_of_Thr_/756602", "title"=>"Growth and development of hepatopancreas and intestine, and intestinal folds height of juvenile Jian carp fed diets containing graded levels of Thr.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-07-26 02:17:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1133003"], "description"=>"<p>Threonine requirements for different fishes.</p>", "links"=>[], "tags"=>["Animal management", "Animal nutrition", "aquaculture", "Fish farming", "biophysics", "Molecular cell biology", "cell division", "Cell growth", "gene expression", "Signal transduction", "Natural materials", "requirements"], "article_id"=>756597, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Lin Feng", "Yan Peng", "Pei Wu", "Kai Hu", "Wei-Dan Jiang", "Yang Liu", "Jun Jiang", "Shu-Hong Li", "Xiao-Qiu Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0069974.t007", "stats"=>{"downloads"=>9, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Threonine_requirements_for_different_fishes_/756597", "title"=>"Threonine requirements for different fishes.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-07-26 02:17:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1133001"], "description"=>"<p>Values are means with standard deviations represented by vertical bars (n = 5). Different letter above bars indicated significant difference among treatments (<i>p</i><0.05). Thr levels were 7.4, 9.1, 12.2, 15.7, 18.6, 22.3 and 25.2 g/kg diet respectively. DI, distal intestine; <i>TOR</i>, <i>target of rapamycin</i>; <i>4E-BP2</i>, <i>eIF4E-binding protein2</i>.</p>", "links"=>[], "tags"=>["Animal management", "Animal nutrition", "aquaculture", "Fish farming", "biophysics", "Molecular cell biology", "cell division", "Cell growth", "gene expression", "Signal transduction", "Natural materials", "dietary", "thr", "distal", "intestine", "juvenile", "jian"], "article_id"=>756596, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Lin Feng", "Yan Peng", "Pei Wu", "Kai Hu", "Wei-Dan Jiang", "Yang Liu", "Jun Jiang", "Shu-Hong Li", "Xiao-Qiu Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0069974.g006", "stats"=>{"downloads"=>2, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_dietary_Thr_on_gene_expression_in_distal_intestine_of_juvenile_Jian_carp_/756596", "title"=>"Effects of dietary Thr on gene expression in distal intestine of juvenile Jian carp.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-26 02:17:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1132997"], "description"=>"<p>Values are means with standard deviations represented by vertical bars (n = 5). Different letter above bars indicated significant difference among treatments (<i>p</i><0.05). Thr levels were 7.4, 9.1, 12.2, 15.7, 18.6, 22.3 and 25.2 g/kg diet respectively. PI, proximal intestine; <i>TOR</i>, <i>target of rapamycin</i>; <i>4E-BP2</i>, <i>eIF4E-binding protein2</i>.</p>", "links"=>[], "tags"=>["Animal management", "Animal nutrition", "aquaculture", "Fish farming", "biophysics", "Molecular cell biology", "cell division", "Cell growth", "gene expression", "Signal transduction", "Natural materials", "dietary", "thr", "proximal", "intestine", "juvenile", "jian"], "article_id"=>756593, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Lin Feng", "Yan Peng", "Pei Wu", "Kai Hu", "Wei-Dan Jiang", "Yang Liu", "Jun Jiang", "Shu-Hong Li", "Xiao-Qiu Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0069974.g004", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_dietary_Thr_on_gene_expression_in_proximal_intestine_of_juvenile_Jian_carp_/756593", "title"=>"Effects of dietary Thr on gene expression in proximal intestine of juvenile Jian carp.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-26 02:17:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1132995"], "description"=>"<p>Values are means with standard deviations represented by vertical bars (n = 5). Different letter above bars indicated significant difference among treatments (<i>p</i><0.05). Thr levels were 7.4, 9.1, 12.2, 15.7, 18.6, 22.3 and 25.2 g/kg diet respectively. <i>TOR</i>, <i>target of rapamycin</i>; <i>4E-BP2</i>, <i>eIF4E-binding protein2</i>.</p>", "links"=>[], "tags"=>["Animal management", "Animal nutrition", "aquaculture", "Fish farming", "biophysics", "Molecular cell biology", "cell division", "Cell growth", "gene expression", "Signal transduction", "Natural materials", "dietary", "thr", "juvenile", "jian"], "article_id"=>756591, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Lin Feng", "Yan Peng", "Pei Wu", "Kai Hu", "Wei-Dan Jiang", "Yang Liu", "Jun Jiang", "Shu-Hong Li", "Xiao-Qiu Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0069974.g002", "stats"=>{"downloads"=>2, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_dietary_Thr_on_gene_expression_in_muscle_of_juvenile_Jian_carp_/756591", "title"=>"Effects of dietary Thr on gene expression in muscle of juvenile Jian carp.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-26 02:17:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1132996"], "description"=>"<p>Values are means with standard deviations represented by vertical bars (n = 5). Different letter above bars indicated significant difference among treatments (<i>p</i><0.05). Thr levels were 7.4, 9.1, 12.2, 15.7, 18.6, 22.3 and 25.2 g/kg diet respectively. <i>TOR</i>, <i>target of rapamycin</i>; <i>4E-BP2</i>, <i>eIF4E-binding protein2</i>.</p>", "links"=>[], "tags"=>["Animal management", "Animal nutrition", "aquaculture", "Fish farming", "biophysics", "Molecular cell biology", "cell division", "Cell growth", "gene expression", "Signal transduction", "Natural materials", "dietary", "thr", "hepatopancreas", "juvenile", "jian"], "article_id"=>756592, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Lin Feng", "Yan Peng", "Pei Wu", "Kai Hu", "Wei-Dan Jiang", "Yang Liu", "Jun Jiang", "Shu-Hong Li", "Xiao-Qiu Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0069974.g003", "stats"=>{"downloads"=>0, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_dietary_Thr_on_gene_expression_in_hepatopancreas_of_juvenile_Jian_carp_/756592", "title"=>"Effects of dietary Thr on gene expression in hepatopancreas of juvenile Jian carp.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-26 02:17:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1132994"], "description"=>"<p>Each point represents the mean of three replicates with fifty fish per replicate.</p>", "links"=>[], "tags"=>["Animal management", "Animal nutrition", "aquaculture", "Fish farming", "biophysics", "Molecular cell biology", "cell division", "Cell growth", "gene expression", "Signal transduction", "Natural materials", "polynomial", "regression", "percent", "juvenile", "jian", "carp", "fed", "graded"], "article_id"=>756590, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Lin Feng", "Yan Peng", "Pei Wu", "Kai Hu", "Wei-Dan Jiang", "Yang Liu", "Jun Jiang", "Shu-Hong Li", "Xiao-Qiu Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0069974.g001", "stats"=>{"downloads"=>1, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Second_degree_polynomial_regression_analysis_of_percent_weight_gain_PWG_for_juvenile_Jian_carp_fed_with_graded_levels_of_Thr_/756590", "title"=>"Second-degree polynomial regression analysis of percent weight gain (PWG) for juvenile Jian carp fed with graded levels of Thr.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-26 02:17:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1133014", "https://ndownloader.figshare.com/files/1133015"], "description"=>"<div><p>This study aimed to investigate the effects of threonine (Thr) on the digestive and absorptive ability, proliferation and differentiation of enterocytes, and gene expression of juvenile Jian carp (<i>Cyprinus carpio</i> var. Jian). First, seven isonitrogenous diets containing graded levels of Thr (7.4–25.2 g/kg diet) were fed to the fishes for 60 days. Second, enterocyte proliferation and differentiation were assayed by culturing enterocytes with graded levels of Thr (0–275 mg/l) <i>in vitro</i>. Finally, enterocytes were cultured with 0 and 205 mg/l Thr to determine protein synthesis. The percent weight gain (PWG), specific growth rate, feed intake, feed efficiency, protein retention value, activities of trypsin, lipase and amylase, weights and protein contents of hepatopancreas and intestine, folds heights, activities of alkaline phosphatase (AKP), γ- glutamyl transpeptidase and Na<sup>+</sup>/K<sup>+</sup>-ATPase in all intestinal segments, glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT) activities in hepatopancreas, and <i>4E-BP2</i> gene expression in muscle, hepatopancreas and intestinal segments were significantly enhanced by Thr (<i>p</i><0.05). However, the plasma ammonia concentration and <i>TOR</i> gene expression decreased (<i>p</i><0.05). <i>In vitro</i>, Thr supplement significantly increased cell numbers, protein content, the activities of GOT, GPT, AKP and Na<sup>+</sup>/K<sup>+</sup>-ATPase, and protein synthesis rate of enterocytes, and decreased LDH activity and ammonia content in cell medium (<i>p</i><0.05). In conclusion, Thr improved growth, digestive and absorptive capacity, enterocyte proliferation and differentiation, and protein synthesis and regulated <i>TOR</i> and <i>4E-BP2</i> gene expression in juvenile Jian carp. The dietary Thr requirement of juvenile Jian carp was 16.25 g/kg diet (51.3 g/kg protein) based on quadratic regression analysis of PWG.</p></div>", "links"=>[], "tags"=>["Animal management", "Animal nutrition", "aquaculture", "Fish farming", "biophysics", "Molecular cell biology", "cell division", "Cell growth", "gene expression", "Signal transduction", "Natural materials", "threonine", "intestinal", "synthesis", "tor", "jian", "carp"], "article_id"=>756604, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Lin Feng", "Yan Peng", "Pei Wu", "Kai Hu", "Wei-Dan Jiang", "Yang Liu", "Jun Jiang", "Shu-Hong Li", "Xiao-Qiu Zhou"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0069974.s001", "https://dx.doi.org/10.1371/journal.pone.0069974.s002"], "stats"=>{"downloads"=>9, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Threonine_Affects_Intestinal_Function_Protein_Synthesis_and_Gene_Expression_of_TOR_in_Jian_Carp_Cyprinus_carpio_var_Jian_/756604", "title"=>"Threonine Affects Intestinal Function, Protein Synthesis and Gene Expression of TOR in Jian Carp (<i>Cyprinus carpio</i> var. Jian)", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-07-26 02:17:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1133009"], "description"=>"<p>Values are means ± SD, n = 5. Mean values with the different superscripts in the same row are significantly different (<i>p</i><0.05).</p>", "links"=>[], "tags"=>["Animal management", "Animal nutrition", "aquaculture", "Fish farming", "biophysics", "Molecular cell biology", "cell division", "Cell growth", "gene expression", "Signal transduction", "Natural materials", "transaminase", "glutamate-pyruvate", "activities", "hepatopancreas", "plasma", "ammonia", "juvenile", "jian", "carp", "fed", "diets", "containing", "graded"], "article_id"=>756601, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Lin Feng", "Yan Peng", "Pei Wu", "Kai Hu", "Wei-Dan Jiang", "Yang Liu", "Jun Jiang", "Shu-Hong Li", "Xiao-Qiu Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0069974.t003", "stats"=>{"downloads"=>2, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Glutamate_oxaloacetate_transaminase_GOT_and_glutamate_pyruvate_transaminase_GPT_activities_in_hepatopancreas_and_plasma_ammonia_concentration_PAC_of_juvenile_Jian_carp_fed_diets_containing_graded_levels_of_Thr_/756601", "title"=>"Glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT) activities in hepatopancreas and plasma ammonia concentration (PAC) of juvenile Jian carp fed diets containing graded levels of Thr.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-07-26 02:17:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1133006"], "description"=>"<p>Values are means ± SD, n = 5. Mean values with the different superscripts in the same row are significantly different (<i>p</i><0.05). AKP, alkaline phosphatase; γ-GT, γ-glutamyl transpeptidase; PI, proximal intestine; MI, mid intestine; DI, distal intestine.</p>", "links"=>[], "tags"=>["Animal management", "Animal nutrition", "aquaculture", "Fish farming", "biophysics", "Molecular cell biology", "cell division", "Cell growth", "gene expression", "Signal transduction", "Natural materials", "enzymes", "activities", "juvenile", "jian", "carp", "fed", "diets", "containing", "graded"], "article_id"=>756599, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Lin Feng", "Yan Peng", "Pei Wu", "Kai Hu", "Wei-Dan Jiang", "Yang Liu", "Jun Jiang", "Shu-Hong Li", "Xiao-Qiu Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0069974.t005", "stats"=>{"downloads"=>3, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_brush_border_enzymes_activities_of_juvenile_Jian_carp_fed_diets_containing_graded_levels_of_Thr_/756599", "title"=>"The brush border enzymes activities of juvenile Jian carp fed diets containing graded levels of Thr.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-07-26 02:17:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1133007"], "description"=>"<p>Values are means ± SD, n = 5. Mean values with the different superscripts in the same row are significantly different (<i>p</i><0.05).</p>", "links"=>[], "tags"=>["Animal management", "Animal nutrition", "aquaculture", "Fish farming", "biophysics", "Molecular cell biology", "cell division", "Cell growth", "gene expression", "Signal transduction", "Natural materials", "activities", "lipase", "amylase", "hepatopancreas", "intestine", "juvenile", "jian", "carp", "fed", "diets", "containing", "graded"], "article_id"=>756600, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Lin Feng", "Yan Peng", "Pei Wu", "Kai Hu", "Wei-Dan Jiang", "Yang Liu", "Jun Jiang", "Shu-Hong Li", "Xiao-Qiu Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0069974.t004", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_activities_of_trypsin_lipase_and_amylase_in_hepatopancreas_and_intestine_of_juvenile_Jian_carp_fed_diets_containing_graded_levels_of_Thr_/756600", "title"=>"The activities of trypsin, lipase and amylase in hepatopancreas and intestine of juvenile Jian carp fed diets containing graded levels of Thr.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-07-26 02:17:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1133004"], "description"=>"<p>Values are means ± SD, n = 4. Mean values with the different superscripts in the same row are significantly different (<i>p</i><0.05). AKP, alkaline phosphatase; GOT, glutamate-oxaloacetate transaminase; GPT, glutamate-pyruvate transaminase.</p>", "links"=>[], "tags"=>["Animal management", "Animal nutrition", "aquaculture", "Fish farming", "biophysics", "Molecular cell biology", "cell division", "Cell growth", "gene expression", "Signal transduction", "Natural materials", "5-diphenyltetrazolium", "bromide", "optical", "enzymes", "activities", "lactate", "dehydrogenase", "ammonia", "enterocytes", "cultured", "graded"], "article_id"=>756598, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Lin Feng", "Yan Peng", "Pei Wu", "Kai Hu", "Wei-Dan Jiang", "Yang Liu", "Jun Jiang", "Shu-Hong Li", "Xiao-Qiu Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0069974.t006", "stats"=>{"downloads"=>3, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_3_4_5_dimethylthiazol_2_yl_2_5_diphenyltetrazolium_bromide_optical_density_MTT_OD_value_protein_content_and_enzymes_activities_of_enterocytes_lactate_dehydrogenase_LDH_activity_and_ammonia_content_in_cell_medium_of_enterocytes_cultured_with_graded_levels/756598", "title"=>"3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide optical density (MTT OD) value, protein content and enzymes activities of enterocytes, lactate dehydrogenase (LDH) activity and ammonia content in cell medium of enterocytes cultured with graded levels of Thr.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-07-26 02:17:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1132998"], "description"=>"<p>Values are means with standard deviations represented by vertical bars (n = 5). Different letter above bars indicated significant difference among treatments (<i>p</i><0.05). Thr levels were 7.4, 9.1, 12.2, 15.7, 18.6, 22.3 and 25.2 g/kg diet respectively. MI, mid intestine; <i>TOR</i>, <i>target of rapamycin</i>; <i>4E-BP2</i>, <i>eIF4E-binding protein2</i>.</p>", "links"=>[], "tags"=>["Animal management", "Animal nutrition", "aquaculture", "Fish farming", "biophysics", "Molecular cell biology", "cell division", "Cell growth", "gene expression", "Signal transduction", "Natural materials", "dietary", "thr", "mid", "intestine", "juvenile", "jian"], "article_id"=>756594, "categories"=>["Medicine", "Biological Sciences"], "users"=>["Lin Feng", "Yan Peng", "Pei Wu", "Kai Hu", "Wei-Dan Jiang", "Yang Liu", "Jun Jiang", "Shu-Hong Li", "Xiao-Qiu Zhou"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0069974.g005", "stats"=>{"downloads"=>1, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_dietary_Thr_on_gene_expression_in_mid_intestine_of_juvenile_Jian_carp_/756594", "title"=>"Effects of dietary Thr on gene expression in mid intestine of juvenile Jian carp.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-26 02:17:05"}

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Relative Metric

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