Rebmab200, a Humanized Monoclonal Antibody Targeting the Sodium Phosphate Transporter NaPi2b Displays Strong Immune Mediated Cytotoxicity against Cancer: A Novel Reagent for Targeted Antibody Therapy of Cancer
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Rebmab200, a Humanized Monoclonal Antibody Targeting the Sodium Phosphate Transporter NaPi2b Displays Strong Immune Mediated Cytotoxicity against Cancer: A Novel Reagent for Targeted Antibody Therapy of Cancer", "type"=>"journal", "authors"=>[{"first_name"=>"Mariana Lopes", "last_name"=>"dos Santos", "scopus_author_id"=>"56363261800"}, {"first_name"=>"Fernanda Perez", "last_name"=>"Yeda", "scopus_author_id"=>"24472213000"}, {"first_name"=>"Lilian Rumi", "last_name"=>"Tsuruta", "scopus_author_id"=>"36963438800"}, {"first_name"=>"Bruno Brasil", "last_name"=>"Horta", "scopus_author_id"=>"55813910200"}, {"first_name"=>"Alécio A.", "last_name"=>"Pimenta", "scopus_author_id"=>"36515437800"}, {"first_name"=>"Theri Leica", "last_name"=>"Degaki", "scopus_author_id"=>"23967813400"}, {"first_name"=>"Ibere C.", "last_name"=>"Soares", "scopus_author_id"=>"7004469920"}, {"first_name"=>"Maria Carolina", "last_name"=>"Tuma", "scopus_author_id"=>"57197103009"}, {"first_name"=>"Oswaldo Keith", "last_name"=>"Okamoto", "scopus_author_id"=>"7005188648"}, {"first_name"=>"Venancio A.F.", "last_name"=>"Alves", "scopus_author_id"=>"15845161000"}, {"first_name"=>"Lloyd J.", "last_name"=>"Old", "scopus_author_id"=>"7101617884"}, {"first_name"=>"Gerd", "last_name"=>"Ritter", "scopus_author_id"=>"7202508138"}, {"first_name"=>"Ana Maria", "last_name"=>"Moro", "scopus_author_id"=>"7006701547"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84881163491", "sgr"=>"84881163491", "pui"=>"369507662", "pmid"=>"23936189", "doi"=>"10.1371/journal.pone.0070332"}, "id"=>"434a13fa-df78-3130-9177-cfc8b42a18b3", "abstract"=>"NaPi2b, a sodium-dependent phosphate transporter, is highly expressed in ovarian carcinomas and is recognized by the murine monoclonal antibody MX35. The antibody had shown excellent targeting to ovarian cancer in several early phase clinical trials but being murine the antibody's full therapeutic potential could not be explored. To overcome this impediment we developed a humanized antibody version named Rebmab200, expressed in human PER.C6® cells and cloned by limiting dilution. In order to select a clone with high therapeutic potential clones were characterized using a series of physicochemical assays, flow cytometry, real-time surface plasmon resonance, glycosylation analyses, immunohistochemistry, antibody-dependent cell-mediated cytotoxicity, complement-dependent-cytotoxicity assays and quantitative PCR. Comparative analyses of Rebmab200 and MX35 monoclonal antibodies demonstrated that the two antibodies had similar specificity for NaPi2b by flow cytometry with a panel of 30 cell lines and maintained similar kinetic parameters. Robust and high producer cell clones potentially suitable for use in manufacturing were obtained. Rebmab200 antibodies were assessed by immunohistochemistry using a large panel of tissues including human carcinomas of ovarian, lung, kidney and breast origin. An assessment of its binding towards 33 normal human organs was performed as well. Rebmab200 showed selected strong reactivity with the tested tumor types but little or no reactivity with the normal tissues tested confirming its potential for targeted therapeutics strategies. The remarkable cytotoxicity shown by Rebmab200 in OVCAR-3 cells is a significant addition to the traits of stability and productivity displayed by the top clones of Rebmab200. Antibody-dependent cell-mediated toxicity functionality was confirmed in repeated assays using cancer cell lines derived from ovary, kidney and lung as targets. To explore use of this antibody in clinical trials, GMP production of Rebmab200 has been initiated. As the next step of development, Phase I clinical trials are now planned for translation of Rebmab200 into the clinic.", "link"=>"http://www.mendeley.com/research/rebmab200-humanized-monoclonal-antibody-targeting-sodium-phosphate-transporter-napi2b-displays-stron-1", "reader_count"=>17, "reader_count_by_academic_status"=>{"Researcher"=>6, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>1, "Student > Master"=>4, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Researcher"=>6, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>3, "Student > Postgraduate"=>1, "Student > Master"=>4, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>9, "Medicine and Dentistry"=>1, "Chemistry"=>2, "Psychology"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>2}, "Psychology"=>{"Psychology"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>9}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"Mexico"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84881163491"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84881163491?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84881163491&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84881163491&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84881163491", "dc:identifier"=>"SCOPUS_ID:84881163491", "eid"=>"2-s2.0-84881163491", "dc:title"=>"Rebmab200, a Humanized Monoclonal Antibody Targeting the Sodium Phosphate Transporter NaPi2b Displays Strong Immune Mediated Cytotoxicity against Cancer: A Novel Reagent for Targeted Antibody Therapy of Cancer", "dc:creator"=>"dos Santos M.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"8", "prism:issueIdentifier"=>"7", "prism:pageRange"=>nil, "prism:coverDate"=>"2013-07-31", "prism:coverDisplayDate"=>"31 July 2013", "prism:doi"=>"10.1371/journal.pone.0070332", "citedby-count"=>"8", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Instituto Butantan", "affiliation-city"=>"Sao Paulo", "affiliation-country"=>"Brazil"}, {"@_fa"=>"true", "affilname"=>"Recepta Biopharma", "affiliation-city"=>"Sao Paulo", "affiliation-country"=>"Brazil"}], "pubmed-id"=>"23936189", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e70332", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0070332", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"7", "year"=>"2013", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"45"}
  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"77", "xml_views"=>"7", "html_views"=>"195"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"77"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"24", "xml_views"=>"3", "html_views"=>"73"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"25", "xml_views"=>"1", "html_views"=>"66"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"32", "xml_views"=>"2", "html_views"=>"169"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"14", "xml_views"=>"2", "html_views"=>"103"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"209"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"177"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"122"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"66"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"131"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"92"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"74"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"73"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"49"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"69"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"61"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"77"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"78"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"69"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"28", "xml_views"=>"8", "html_views"=>"43"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"38", "xml_views"=>"20", "html_views"=>"52"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"34", "xml_views"=>"23", "html_views"=>"56"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"68"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"39"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"3", "html_views"=>"40"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"35"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"6", "html_views"=>"17"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"17"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"21", "xml_views"=>"1", "html_views"=>"28"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"20"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"8"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1138419"], "description"=>"<p>Immunoreactivity of MX35 and Rebmab200 in live cells by flow cytometry.</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Recombinant proteins", "glycobiology", "immunochemistry", "biotechnology", "immunology", "Immune cells", "Antibody-producing cells", "immunoglobulins", "Molecular cell biology", "cytometry", "flow cytometry", "Applied chemistry", "Chemical properties", "Physicochemical properties", "Anatomy and physiology", "Immune physiology", "antibodies", "oncology", "Cancer treatment", "Antibody therapy", "immunotherapy", "Cancers and neoplasms", "Gynecological tumors", "Ovarian cancer", "Basic cancer research", "Cancer detection and diagnosis", "Oncology agents", "mx35", "rebmab200", "cells"], "article_id"=>760813, "categories"=>["Medicine", "Chemistry", "Biological Sciences"], "users"=>["Mariana Lopes dos Santos", "Fernanda Perez Yeda", "Lilian Rumi Tsuruta", "Bruno Brasil Horta", "Alécio A. Pimenta Jr", "Theri Leica Degaki", "Ibere C. Soares", "Maria Carolina Tuma", "Oswaldo Keith Okamoto", "Venancio A. F. Alves", "Lloyd J. Old", "Gerd Ritter", "Ana Maria Moro"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0070332.t001", "stats"=>{"downloads"=>10, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Immunoreactivity_of_MX35_and_Rebmab200_in_live_cells_by_flow_cytometry_/760813", "title"=>"Immunoreactivity of MX35 and Rebmab200 in live cells by flow cytometry.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-07-31 02:02:33"}
  • {"files"=>["https://ndownloader.figshare.com/files/1138418"], "description"=>"<p><b>GAPDH was used as a reference gene.</b> Vector represents PER.C6®cells transfected with mock vector; PER.C6® corresponds to the parental cell. Error bars represent the standard deviation of triplicates. Qp represents specific productivity levels.</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Recombinant proteins", "glycobiology", "immunochemistry", "biotechnology", "immunology", "Immune cells", "Antibody-producing cells", "immunoglobulins", "Molecular cell biology", "cytometry", "flow cytometry", "Applied chemistry", "Chemical properties", "Physicochemical properties", "Anatomy and physiology", "Immune physiology", "antibodies", "oncology", "Cancer treatment", "Antibody therapy", "immunotherapy", "Cancers and neoplasms", "Gynecological tumors", "Ovarian cancer", "Basic cancer research", "Cancer detection and diagnosis", "Oncology agents", "hc", "lc", "mrna", "rebmab200", "clones"], "article_id"=>760812, "categories"=>["Medicine", "Chemistry", "Biological Sciences"], "users"=>["Mariana Lopes dos Santos", "Fernanda Perez Yeda", "Lilian Rumi Tsuruta", "Bruno Brasil Horta", "Alécio A. Pimenta Jr", "Theri Leica Degaki", "Ibere C. Soares", "Maria Carolina Tuma", "Oswaldo Keith Okamoto", "Venancio A. F. Alves", "Lloyd J. Old", "Gerd Ritter", "Ana Maria Moro"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0070332.g005", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Immunoglobulin_HC_and_LC_mRNA_levels_and_productivity_of_the_most_representative_Rebmab200_clones_as_determined_by_RT_qPCR_/760812", "title"=>"Immunoglobulin HC and LC mRNA levels and productivity of the most representative Rebmab200 clones as determined by RT-qPCR.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-31 02:02:33"}
  • {"files"=>["https://ndownloader.figshare.com/files/1138415"], "description"=>"<p>The % of cytotoxicity represents antibody-mediated cell lysis measured by release of <sup>51</sup>Cr from labeled tumor cells. Effector cells were obtained from donated human blood. The assay was repeated with MCF-7 cells, a NaPi2b negative and Le<sup>Y</sup> positive (Rebmab100 antigen) tumor cell line, showing no ADCC results (data not shown).</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Recombinant proteins", "glycobiology", "immunochemistry", "biotechnology", "immunology", "Immune cells", "Antibody-producing cells", "immunoglobulins", "Molecular cell biology", "cytometry", "flow cytometry", "Applied chemistry", "Chemical properties", "Physicochemical properties", "Anatomy and physiology", "Immune physiology", "antibodies", "oncology", "Cancer treatment", "Antibody therapy", "immunotherapy", "Cancers and neoplasms", "Gynecological tumors", "Ovarian cancer", "Basic cancer research", "Cancer detection and diagnosis", "Oncology agents", "rebmab200", "produced", "clones", "ovcar-3", "sk-ov-6", "sk-lc-1", "sk-rc-18", "cancer", "lines"], "article_id"=>760809, "categories"=>["Medicine", "Chemistry", "Biological Sciences"], "users"=>["Mariana Lopes dos Santos", "Fernanda Perez Yeda", "Lilian Rumi Tsuruta", "Bruno Brasil Horta", "Alécio A. Pimenta Jr", "Theri Leica Degaki", "Ibere C. Soares", "Maria Carolina Tuma", "Oswaldo Keith Okamoto", "Venancio A. F. Alves", "Lloyd J. Old", "Gerd Ritter", "Ana Maria Moro"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0070332.g004", "stats"=>{"downloads"=>2, "page_views"=>42, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ADCC_activity_of_Rebmab200_produced_by_the_top_three_clones_in_two_ovarian_OVCAR_3_A_and_SK_OV_6_B_one_lung_SK_LC_1_C_and_one_renal_SK_RC_18_D_cancer_cell_lines_in_comparison_to_Rebmab100_/760809", "title"=>"ADCC activity of Rebmab200 produced by the top three clones in two ovarian, OVCAR-3 (A) and SK-OV-6 (B), one lung, SK-LC-1 (C), and one renal, SK-RC-18 (D), cancer cell lines in comparison to Rebmab100.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-31 02:02:33"}
  • {"files"=>["https://ndownloader.figshare.com/files/1138413"], "description"=>"<p>Serial sections of serous papillary ovary adenocarcinoma (A), clear cell renal carcinoma (B), large cell poorly differentiated carcinoma of the lung (C) and invasive ductal carcinoma of the breast (D). Original magnification = 100×.</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Recombinant proteins", "glycobiology", "immunochemistry", "biotechnology", "immunology", "Immune cells", "Antibody-producing cells", "immunoglobulins", "Molecular cell biology", "cytometry", "flow cytometry", "Applied chemistry", "Chemical properties", "Physicochemical properties", "Anatomy and physiology", "Immune physiology", "antibodies", "oncology", "Cancer treatment", "Antibody therapy", "immunotherapy", "Cancers and neoplasms", "Gynecological tumors", "Ovarian cancer", "Basic cancer research", "Cancer detection and diagnosis", "Oncology agents", "immuno-affinity", "mx35", "dilution", "rebmab200", "parallel", "reactions", "buffers", "amplification"], "article_id"=>760807, "categories"=>["Medicine", "Chemistry", "Biological Sciences"], "users"=>["Mariana Lopes dos Santos", "Fernanda Perez Yeda", "Lilian Rumi Tsuruta", "Bruno Brasil Horta", "Alécio A. Pimenta Jr", "Theri Leica Degaki", "Ibere C. Soares", "Maria Carolina Tuma", "Oswaldo Keith Okamoto", "Venancio A. F. Alves", "Lloyd J. Old", "Gerd Ritter", "Ana Maria Moro"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0070332.g003", "stats"=>{"downloads"=>2, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparison_of_the_immuno_affinity_of_MX35_left_at_1_50_dilution_and_Rebmab200_right_at_1_100_dilution_in_parallel_reactions_using_the_same_buffers_and_amplification_and_development_conditions_/760807", "title"=>"Comparison of the immuno-affinity of MX35 (left) at 1/50 dilution and Rebmab200 (right) at 1/100 dilution in parallel reactions using the same buffers and amplification and development conditions.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-31 02:02:33"}
  • {"files"=>["https://ndownloader.figshare.com/files/1138409"], "description"=>"<p>The % of cytotoxicity represents antibody-mediated cell lysis measured by release of <sup>51</sup>Cr from labeled ovarian cancer cells (OVCAR-3). Effector cells were obtained from donated human peripheral blood. Rebmab100 was used as a positive control, and Zenapax (Roche) was used as a negative control. The assay was repeated with MCF-7 cells, a NaPi2b negative and Le<sup>Y</sup> positive (Rebmab100 antigen) tumor cell line, showing no ADCC results (data not shown).</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Recombinant proteins", "glycobiology", "immunochemistry", "biotechnology", "immunology", "Immune cells", "Antibody-producing cells", "immunoglobulins", "Molecular cell biology", "cytometry", "flow cytometry", "Applied chemistry", "Chemical properties", "Physicochemical properties", "Anatomy and physiology", "Immune physiology", "antibodies", "oncology", "Cancer treatment", "Antibody therapy", "immunotherapy", "Cancers and neoplasms", "Gynecological tumors", "Ovarian cancer", "Basic cancer research", "Cancer detection and diagnosis", "Oncology agents", "adcc", "mx35", "rebmab200", "mab"], "article_id"=>760803, "categories"=>["Medicine", "Chemistry", "Biological Sciences"], "users"=>["Mariana Lopes dos Santos", "Fernanda Perez Yeda", "Lilian Rumi Tsuruta", "Bruno Brasil Horta", "Alécio A. Pimenta Jr", "Theri Leica Degaki", "Ibere C. Soares", "Maria Carolina Tuma", "Oswaldo Keith Okamoto", "Venancio A. F. Alves", "Lloyd J. Old", "Gerd Ritter", "Ana Maria Moro"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0070332.g002", "stats"=>{"downloads"=>5, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparison_of_ADCC_activity_between_MX35_and_Rebmab200_stable_pool_over_a_range_of_mAb_concentrations_/760803", "title"=>"Comparison of ADCC activity between MX35 and Rebmab200 (stable pool) over a range of mAb concentrations.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-31 02:02:33"}
  • {"files"=>["https://ndownloader.figshare.com/files/1138408"], "description"=>"<p>MX35 (A) and Rebmab200 (B) were injected at concentrations ranging from 5 to 80 nM. After a 10 min association phase, the dissociation phase was followed for additional 10 min. Following double subtractive referencing, the curves were plotted using a 1∶1 Langmuir binding model, using Biacore T100 Evaluation Software. The solid line represents the experimental data and the dotted line the mathematical model for the binding of MX35 and Rebmab200 to the synthetic NaPi2b epitope.</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Recombinant proteins", "glycobiology", "immunochemistry", "biotechnology", "immunology", "Immune cells", "Antibody-producing cells", "immunoglobulins", "Molecular cell biology", "cytometry", "flow cytometry", "Applied chemistry", "Chemical properties", "Physicochemical properties", "Anatomy and physiology", "Immune physiology", "antibodies", "oncology", "Cancer treatment", "Antibody therapy", "immunotherapy", "Cancers and neoplasms", "Gynecological tumors", "Ovarian cancer", "Basic cancer research", "Cancer detection and diagnosis", "Oncology agents", "fitted", "spr", "murine", "mx35", "rebmab200", "binding", "immobilized", "synthetic", "napi2b"], "article_id"=>760802, "categories"=>["Medicine", "Chemistry", "Biological Sciences"], "users"=>["Mariana Lopes dos Santos", "Fernanda Perez Yeda", "Lilian Rumi Tsuruta", "Bruno Brasil Horta", "Alécio A. Pimenta Jr", "Theri Leica Degaki", "Ibere C. Soares", "Maria Carolina Tuma", "Oswaldo Keith Okamoto", "Venancio A. F. Alves", "Lloyd J. Old", "Gerd Ritter", "Ana Maria Moro"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0070332.g001", "stats"=>{"downloads"=>3, "page_views"=>26, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Global_fitted_dotted_line_SPR_data_of_murine_MX35_and_Rebmab200_binding_to_immobilized_synthetic_NaPi2b_epitope_/760802", "title"=>"Global fitted (dotted line) SPR data of murine MX35 and Rebmab200 binding to immobilized synthetic NaPi2b epitope.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-07-31 02:02:33"}
  • {"files"=>["https://ndownloader.figshare.com/files/1138420"], "description"=>"<p>Immunoreactivity of MX35 and Rebmab200 in tumor tissues by immunohistochemistry.</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "Recombinant proteins", "glycobiology", "immunochemistry", "biotechnology", "immunology", "Immune cells", "Antibody-producing cells", "immunoglobulins", "Molecular cell biology", "cytometry", "flow cytometry", "Applied chemistry", "Chemical properties", "Physicochemical properties", "Anatomy and physiology", "Immune physiology", "antibodies", "oncology", "Cancer treatment", "Antibody therapy", "immunotherapy", "Cancers and neoplasms", "Gynecological tumors", "Ovarian cancer", "Basic cancer research", "Cancer detection and diagnosis", "Oncology agents", "mx35", "rebmab200", "tissues"], "article_id"=>760814, "categories"=>["Medicine", "Chemistry", "Biological Sciences"], "users"=>["Mariana Lopes dos Santos", "Fernanda Perez Yeda", "Lilian Rumi Tsuruta", "Bruno Brasil Horta", "Alécio A. Pimenta Jr", "Theri Leica Degaki", "Ibere C. Soares", "Maria Carolina Tuma", "Oswaldo Keith Okamoto", "Venancio A. F. Alves", "Lloyd J. Old", "Gerd Ritter", "Ana Maria Moro"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0070332.t002", "stats"=>{"downloads"=>8, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Immunoreactivity_of_MX35_and_Rebmab200_in_tumor_tissues_by_immunohistochemistry_/760814", "title"=>"Immunoreactivity of MX35 and Rebmab200 in tumor tissues by immunohistochemistry.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-07-31 02:02:33"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"58", "full-text"=>"49", "pdf"=>"27", "abstract"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"38", "full-text"=>"40", "pdf"=>"25", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"37", "full-text"=>"33", "pdf"=>"140", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"22", "full-text"=>"18", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"25", "full-text"=>"23", "pdf"=>"29", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"18", "full-text"=>"18", "pdf"=>"10", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"20", "full-text"=>"24", "pdf"=>"9", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"21", "full-text"=>"20", "pdf"=>"12", "abstract"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"26", "full-text"=>"37", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"25", "full-text"=>"34", "pdf"=>"31", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"20", "full-text"=>"24", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"24", "full-text"=>"33", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"18", "full-text"=>"18", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"18", "full-text"=>"19", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"21", "full-text"=>"26", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"25", "full-text"=>"27", "pdf"=>"21", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"20", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"20", "full-text"=>"17", "pdf"=>"9", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"19", "full-text"=>"13", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"24", "full-text"=>"25", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"21", "full-text"=>"22", "pdf"=>"9", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"15", "full-text"=>"18", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"27", "full-text"=>"24", "pdf"=>"18", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"17", "full-text"=>"20", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"26", "full-text"=>"26", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"17", "full-text"=>"18", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"17", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"17", "full-text"=>"19", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"16", "full-text"=>"15", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"19", "full-text"=>"17", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"15", "full-text"=>"20", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"16", "full-text"=>"11", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"17", "full-text"=>"21", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"19", "full-text"=>"21", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"16", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"20", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"12", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"14", "pdf"=>"19", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Physiology", "average_usage"=>[256, 449, 572, 676, 775, 866, 955, 1041, 1127, 1213, 1290, 1370, 1437]}, {"subject_area"=>"/Biology and life sciences/Toxicology", "average_usage"=>[247, 444, 560, 669, 769, 863, 947, 1036, 1129, 1204, 1296, 1374, 1437]}, {"subject_area"=>"/Medicine and health sciences/Oncology", "average_usage"=>[249, 468, 599, 718, 820, 920, 1008, 1093, 1181, 1281, 1357, 1444, 1517]}]}
Loading … Spinner
There are currently no alerts