Direct Detection of Alternative Open Reading Frames Translation Products in Human Significantly Expands the Proteome
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Direct Detection of Alternative Open Reading Frames Translation Products in Human Significantly Expands the Proteome", "type"=>"journal", "authors"=>[{"first_name"=>"Benoît", "last_name"=>"Vanderperre", "scopus_author_id"=>"57194759184"}, {"first_name"=>"Jean François", "last_name"=>"Lucier", "scopus_author_id"=>"9744220200"}, {"first_name"=>"Cyntia", "last_name"=>"Bissonnette", "scopus_author_id"=>"13411465000"}, {"first_name"=>"Julie", "last_name"=>"Motard", "scopus_author_id"=>"16025150100"}, {"first_name"=>"Guillaume", "last_name"=>"Tremblay", "scopus_author_id"=>"57196801622"}, {"first_name"=>"Solène", "last_name"=>"Vanderperre", "scopus_author_id"=>"55820701500"}, {"first_name"=>"Maxence", "last_name"=>"Wisztorski", "scopus_author_id"=>"56028414300"}, {"first_name"=>"Michel", "last_name"=>"Salzet", "scopus_author_id"=>"7007171556"}, {"first_name"=>"François Michel", "last_name"=>"Boisvert", "scopus_author_id"=>"6602497276"}, {"first_name"=>"Xavier", "last_name"=>"Roucou", "scopus_author_id"=>"6603409209"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"369566388", "sgr"=>"84881529829", "pmid"=>"23950983", "scopus"=>"2-s2.0-84881529829", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "doi"=>"10.1371/journal.pone.0070698", "issn"=>"19326203"}, "id"=>"7de937e8-4395-3998-9f8e-947026d7a241", "abstract"=>"A fully mature mRNA is usually associated to a reference open reading frame encoding a single protein. Yet, mature mRNAs contain unconventional alternative open reading frames (AltORFs) located in untranslated regions (UTRs) or overlapping the reference ORFs (RefORFs) in non-canonical +2 and +3 reading frames. Although recent ribosome profiling and footprinting approaches have suggested the significant use of unconventional translation initiation sites in mammals, direct evidence of large-scale alternative protein expression at the proteome level is still lacking. To determine the contribution of alternative proteins to the human proteome, we generated a database of predicted human AltORFs revealing a new proteome mainly composed of small proteins with a median length of 57 amino acids, compared to 344 amino acids for the reference proteome. We experimentally detected a total of 1,259 alternative proteins by mass spectrometry analyses of human cell lines, tissues and fluids. In plasma and serum, alternative proteins represent up to 55% of the proteome and may be a potential unsuspected new source for biomarkers. We observed constitutive co-expression of RefORFs and AltORFs from endogenous genes and from transfected cDNAs, including tumor suppressor p53, and provide evidence that out-of-frame clones representing AltORFs are mistakenly rejected as false positive in cDNAs screening assays. Functional importance of alternative proteins is strongly supported by significant evolutionary conservation in vertebrates, invertebrates, and yeast. Our results imply that coding of multiple proteins in a single gene by the use of AltORFs may be a common feature in eukaryotes, and confirm that translation of unconventional ORFs generates an as yet unexplored proteome.", "link"=>"http://www.mendeley.com/research/direct-detection-alternative-open-reading-frames-translation-products-human-significantly-expands-pr", "reader_count"=>137, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>8, "Researcher"=>32, "Student > Doctoral Student"=>5, "Student > Ph. D. Student"=>32, "Student > Postgraduate"=>4, "Student > Master"=>28, "Other"=>5, "Student > Bachelor"=>14, "Lecturer"=>2, "Professor"=>5}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>8, "Researcher"=>32, "Student > Doctoral Student"=>5, "Student > Ph. D. Student"=>32, "Student > Postgraduate"=>4, "Student > Master"=>28, "Other"=>5, "Student > Bachelor"=>14, "Lecturer"=>2, "Professor"=>5}, "reader_count_by_subject_area"=>{"Unspecified"=>4, "Agricultural and Biological Sciences"=>67, "Chemical Engineering"=>1, "Chemistry"=>4, "Computer Science"=>3, "Engineering"=>3, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>39, "Mathematics"=>2, "Medicine and Dentistry"=>9, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Social Sciences"=>1, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>9}, "Social Sciences"=>{"Social Sciences"=>1}, "Mathematics"=>{"Mathematics"=>2}, "Unspecified"=>{"Unspecified"=>4}, "Environmental Science"=>{"Environmental Science"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "Chemical Engineering"=>{"Chemical Engineering"=>1}, "Engineering"=>{"Engineering"=>3}, "Chemistry"=>{"Chemistry"=>4}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>67}, "Computer Science"=>{"Computer Science"=>3}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>39}}, "reader_count_by_country"=>{"Canada"=>2, "Netherlands"=>1, "Turkey"=>1, "Hungary"=>1, "United States"=>2, "China"=>1, "Ireland"=>1, "United Kingdom"=>2, "France"=>2, "Switzerland"=>1, "Germany"=>1, "Spain"=>1}, "group_count"=>8}

CrossRef

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84881529829"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84881529829?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84881529829&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84881529829&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84881529829", "dc:identifier"=>"SCOPUS_ID:84881529829", "eid"=>"2-s2.0-84881529829", "dc:title"=>"Direct Detection of Alternative Open Reading Frames Translation Products in Human Significantly Expands the Proteome", "dc:creator"=>"Vanderperre B.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"8", "prism:issueIdentifier"=>"8", "prism:pageRange"=>nil, "prism:coverDate"=>"2013-08-12", "prism:coverDisplayDate"=>"12 August 2013", "prism:doi"=>"10.1371/journal.pone.0070698", "citedby-count"=>"75", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Université de Sherbrooke, Faculté de Médecine et des Sciences de la Santé", "affiliation-city"=>"Sherbrooke", "affiliation-country"=>"Canada"}], "pubmed-id"=>"23950983", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e70698", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0070698", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"8", "year"=>"2013", "pdf_views"=>"87", "xml_views"=>"10", "html_views"=>"396"}
  • {"month"=>"9", "year"=>"2013", "pdf_views"=>"55", "xml_views"=>"2", "html_views"=>"233"}
  • {"month"=>"10", "year"=>"2013", "pdf_views"=>"62", "xml_views"=>"4", "html_views"=>"223"}
  • {"month"=>"11", "year"=>"2013", "pdf_views"=>"56", "xml_views"=>"0", "html_views"=>"184"}
  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"40", "xml_views"=>"0", "html_views"=>"171"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"76", "xml_views"=>"1", "html_views"=>"259"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"56", "xml_views"=>"3", "html_views"=>"187"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"59", "xml_views"=>"1", "html_views"=>"157"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"67", "xml_views"=>"2", "html_views"=>"223"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"62", "xml_views"=>"0", "html_views"=>"184"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"293"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"30", "xml_views"=>"1", "html_views"=>"215"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"14", "xml_views"=>"4", "html_views"=>"140"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"33", "xml_views"=>"1", "html_views"=>"185"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"32", "xml_views"=>"2", "html_views"=>"174"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"41", "xml_views"=>"2", "html_views"=>"214"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"139"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"28", "xml_views"=>"0", "html_views"=>"124"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"91"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"135"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"116"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"103"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"118"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"110"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"22", "xml_views"=>"1", "html_views"=>"73"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"111"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"174"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"154"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"160"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"29", "xml_views"=>"0", "html_views"=>"119"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"101"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"92"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"72"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"24", "xml_views"=>"0", "html_views"=>"96"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"90"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"71"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"21", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"107"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"110"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"86"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"110"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"166"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"149"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"29", "xml_views"=>"1", "html_views"=>"168"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"92"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"22", "xml_views"=>"2", "html_views"=>"87"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"79"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"26", "xml_views"=>"1", "html_views"=>"78"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"19", "xml_views"=>"2", "html_views"=>"96"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"124"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"90"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"50"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"24"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"17", "xml_views"=>"2", "html_views"=>"46"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"31", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"21", "xml_views"=>"5", "html_views"=>"42"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"32", "xml_views"=>"1", "html_views"=>"54"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"49"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"30", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"58"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"67"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"25"}

Figshare

  • {"files"=>["http://files.figshare.com/1151392/Figure_4.tif"], "pos_in_sequence"=>0, "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "users"=>["Guillaume Tremblay", "Xavier Roucou", "Benoît Vanderperre", "Cyntia Bissonnette", "Solène Vanderperre", "François-Michel Boisvert", "Michel Salzet", "Jean-François Lucier", "Maxence Wisztorski", "Julie Motard"], "links"=>[], "tags"=>["experiments", "transfection", "proteins", "proteome", "genetics", "Protein classes", "Plasma proteins", "protein synthesis", "Protein translation", "Information technology", "databases", "cdna", "Spectrometric identification of proteins", "gene expression", "Molecular cell biology", "Biochemistry", "Proteomic databases", "proteomics"], "title"=>"<p>Co-expression of alternative and reference proteins in cDNA transfection experiments is common.</p>", "figshare_url"=>"http://figshare.com/articles/_Co_expression_of_alternative_and_reference_proteins_in_cDNA_transfection_experiments_is_common_/770912", "defined_type"=>1, "doi"=>["http://dx.doi.org/10.1371/journal.pone.0070698.g004"], "published_date"=>"2013-08-12 07:01:01", "article_id"=>770912, "categories"=>["Biological Sciences", "Information And Computing Sciences"], "description"=>"<p>(<i>A</i>) Distribution of the number of predicted RefORFs-contained AltORFs per gene in the human genome. Top, schematic representation of a mRNA with a RefORF (grey)-containing AltORF (green). By definition, RefORFs are present in the +1 reading frame and AltORFs are present in the non-canonical +2 and +3 reading frames. (<i>B</i>) Strategy to detect the co-expression of reference and alternative proteins in cDNA transfection experiments. HA and GFP tags permit the detection of reference and alternative proteins, respectively. Top, graphical representation of a mRNA with a RefORF-contained AltORF. Middle, typical cDNA construct used in transfection experiments. Bottom, representation of constructs used in (<i>C</i>). (<i>C</i>) Western blot analyses of HA-tagged LGALS3BP (Lectin galactoside-binding soluble 3 binding protein), VEGFC (vascular endothelium growth factor), p53 (cellular tumor antigen p53), CDC42 (cell division cycle 42), BDKRB2 (bradykinin receptor), and SRSF1 (serine/arginine-rich splicing factor 1), and their respective GFP-tagged alternative proteins using anti-HA and anti-GFP antibodies (top panels). Bottom panels show the cellular distribution of alternative proteins by confocal fluorescence microscopy (differential interference contrast and Hoechst, left panels; GFP, right panels). Scale bar: 10 μm.</p>"}
  • {"files"=>["http://files.figshare.com/1151393/Figure_5.tif"], "pos_in_sequence"=>0, "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "users"=>["Guillaume Tremblay", "Xavier Roucou", "Benoît Vanderperre", "Cyntia Bissonnette", "Solène Vanderperre", "François-Michel Boisvert", "Michel Salzet", "Jean-François Lucier", "Maxence Wisztorski", "Julie Motard"], "links"=>[], "tags"=>["altmrvi1", "interacts", "proteins", "proteome", "gene expression", "Protein classes", "Plasma proteins", "protein synthesis", "Protein translation", "Information technology", "databases", "genetics", "Spectrometric identification of proteins", "Molecular cell biology", "Biochemistry", "Proteomic databases", "proteomics"], "title"=>"<p>The alternative protein AltMRVI1 interacts with BRCA1.</p>", "figshare_url"=>"http://figshare.com/articles/_The_alternative_protein_AltMRVI1_interacts_with_BRCA1_/770913", "defined_type"=>1, "doi"=>["http://dx.doi.org/10.1371/journal.pone.0070698.g005"], "published_date"=>"2013-08-12 07:01:01", "article_id"=>770913, "categories"=>["Biological Sciences", "Information And Computing Sciences"], "description"=>"<p>(<i>A</i>) Sequence alignment between the third BRCA1 interacting protein 3 (BRCA1 IP3) identified by Liu et al by Y2H <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070698#pone.0070698-Liu1\" target=\"_blank\">[40]</a> and AltMRVI1. (<i>B</i>) Localization of BRCA1 and AltMRVI1<sup>EGFP</sup> in the nucleus. HeLa cells transfected with AltMRVI1<sup>EGFP</sup> (green channel) were immunostained with anti-BRCA1 (red channel) antibodies. Nuclei were stained with Hoechst (blue channel). Scale bar, 10 &#956;m. (<i>C</i>) Co-immunoprecipitation with anti-GFP antibodies was performed using lysates from HeLa cells expressing AltMRVI1<sup>EGFP</sup>. Molecular weight markers in kDa are indicated on the right.</p>"}
  • {"files"=>["http://files.figshare.com/1151394/Figure_6.tif"], "pos_in_sequence"=>0, "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "users"=>["Guillaume Tremblay", "Xavier Roucou", "Benoît Vanderperre", "Cyntia Bissonnette", "Solène Vanderperre", "François-Michel Boisvert", "Michel Salzet", "Jean-François Lucier", "Maxence Wisztorski", "Julie Motard"], "links"=>[], "tags"=>["proteins", "proteome", "conserved", "gene expression", "Protein classes", "Plasma proteins", "protein synthesis", "Protein translation", "Information technology", "databases", "genetics", "Spectrometric identification of proteins", "Molecular cell biology", "Biochemistry", "Proteomic databases", "proteomics"], "title"=>"<p>Alternative proteins are conserved from human to yeast.</p>", "figshare_url"=>"http://figshare.com/articles/_Alternative_proteins_are_conserved_from_human_to_yeast_/770914", "defined_type"=>1, "doi"=>["http://dx.doi.org/10.1371/journal.pone.0070698.g006"], "published_date"=>"2013-08-12 07:01:01", "article_id"=>770914, "categories"=>["Biological Sciences", "Information And Computing Sciences"], "description"=>"<p>Percent identity of predicted alternative proteins or reference proteins from different eukaryote species analysed with BLASTP (cutoff expectation value of ≤10<sup>&#8722;4</sup>) against human corresponding proteins. The number of homologous proteins displaying each percent identity is shown on the y axis. The dotted black bar indicates the median percent identity for each species. Insets indicate the total number of conserved proteins.</p>"}
  • {"files"=>["http://files.figshare.com/1151395/Table_1.xls"], "pos_in_sequence"=>0, "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "users"=>["Guillaume Tremblay", "Xavier Roucou", "Benoît Vanderperre", "Cyntia Bissonnette", "Solène Vanderperre", "François-Michel Boisvert", "Michel Salzet", "Jean-François Lucier", "Maxence Wisztorski", "Julie Motard"], "links"=>[], "tags"=>["proteins", "proteome", "gene expression", "Protein classes", "Plasma proteins", "protein synthesis", "Protein translation", "Information technology", "databases", "genetics", "Spectrometric identification of proteins", "Molecular cell biology", "Biochemistry", "Proteomic databases", "proteomics"], "title"=>"<p>Summary of LC-MS/MS analyses of human samples.</p>", "figshare_url"=>"http://figshare.com/articles/_Summary_of_LC_MS_MS_analyses_of_human_samples_/770915", "defined_type"=>3, "doi"=>["http://dx.doi.org/10.1371/journal.pone.0070698.t001"], "published_date"=>"2013-08-12 07:01:01", "article_id"=>770915, "categories"=>["Biological Sciences", "Information And Computing Sciences"], "description"=>"<span class=\"pl_xls_label\">a</span><p >Total identified proteins &#8202;=&#8202; alternative + reference proteins.</p><span class=\"pl_xls_label\">b</span><p >PeptideAtlas accession numbers associated with lung, cerebrospinal fluid, urine, plasma and serum samples are indicated in the Methods section. The Serum total line indicates the sum of distinct proteins detected in Serum A, B, C and D.</p>"}
  • {"files"=>["http://files.figshare.com/1565504/Figure_S1.tif", "http://files.figshare.com/1565507/Figure_S2.tif", "http://files.figshare.com/1565511/Table_S1.xlsx", "http://files.figshare.com/1565512/Table_S2.xlsx", "http://files.figshare.com/1565515/Table_S3.xlsx", "http://files.figshare.com/1565517/Table_S4.xlsx", "http://files.figshare.com/1565518/Table_S5.doc", "http://files.figshare.com/1565520/Database_S1.xlsx", "http://files.figshare.com/1565523/Database_S2.xlsx", "http://files.figshare.com/1565526/Database_S3.xlsx", "http://files.figshare.com/1565527/Database_S4.xlsx", "http://files.figshare.com/1565528/Database_S5.xlsx", "http://files.figshare.com/1565529/Database_S6.xlsx", "http://files.figshare.com/1565530/Database_S7.xlsx", "http://files.figshare.com/1565531/Database_S8.xlsx"], "pos_in_sequence"=>0, "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "users"=>["Guillaume Tremblay", "Xavier Roucou", "Benoît Vanderperre", "Cyntia Bissonnette", "Solène Vanderperre", "François-Michel Boisvert", "Michel Salzet", "Jean-François Lucier", "Maxence Wisztorski", "Julie Motard"], "links"=>[], "tags"=>["proteins", "proteome", "detection", "gene expression", "frames", "Protein classes", "Plasma proteins", "protein synthesis", "Protein translation", "Information technology", "databases", "genetics", "Spectrometric identification of proteins", "Molecular cell biology", "Biochemistry", "expands", "Proteomic databases", "proteomics"], "title"=>"<p>Direct Detection of Alternative Open Reading Frames Translation Products in Human Significantly Expands the Proteome</p>", "figshare_url"=>"http://figshare.com/articles/_Direct_Detection_of_Alternative_Open_Reading_Frames_Translation_Products_in_Human_Significantly_Expands_the_Proteome_/1083610", "defined_type"=>4, "doi"=>["http://dx.doi.org/10.1371/journal.pone.0070698.s001", "http://dx.doi.org/10.1371/journal.pone.0070698.s002", "http://dx.doi.org/10.1371/journal.pone.0070698.s003", "http://dx.doi.org/10.1371/journal.pone.0070698.s004", "http://dx.doi.org/10.1371/journal.pone.0070698.s005", "http://dx.doi.org/10.1371/journal.pone.0070698.s006", "http://dx.doi.org/10.1371/journal.pone.0070698.s007", "http://dx.doi.org/10.1371/journal.pone.0070698.s008", "http://dx.doi.org/10.1371/journal.pone.0070698.s009", "http://dx.doi.org/10.1371/journal.pone.0070698.s010", "http://dx.doi.org/10.1371/journal.pone.0070698.s011", "http://dx.doi.org/10.1371/journal.pone.0070698.s012", "http://dx.doi.org/10.1371/journal.pone.0070698.s013", "http://dx.doi.org/10.1371/journal.pone.0070698.s014", "http://dx.doi.org/10.1371/journal.pone.0070698.s015"], "published_date"=>"2013-08-12 08:57:27", "article_id"=>1083610, "categories"=>["Biological Sciences"], "description"=>"<div><p>A fully mature mRNA is usually associated to a reference open reading frame encoding a single protein. Yet, mature mRNAs contain unconventional alternative open reading frames (AltORFs) located in untranslated regions (UTRs) or overlapping the reference ORFs (RefORFs) in non-canonical +2 and +3 reading frames. Although recent ribosome profiling and footprinting approaches have suggested the significant use of unconventional translation initiation sites in mammals, direct evidence of large-scale alternative protein expression at the proteome level is still lacking. To determine the contribution of alternative proteins to the human proteome, we generated a database of predicted human AltORFs revealing a new proteome mainly composed of small proteins with a median length of 57 amino acids, compared to 344 amino acids for the reference proteome. We experimentally detected a total of 1,259 alternative proteins by mass spectrometry analyses of human cell lines, tissues and fluids. In plasma and serum, alternative proteins represent up to 55% of the proteome and may be a potential unsuspected new source for biomarkers. We observed constitutive co-expression of RefORFs and AltORFs from endogenous genes and from transfected cDNAs, including tumor suppressor p53, and provide evidence that out-of-frame clones representing AltORFs are mistakenly rejected as false positive in cDNAs screening assays. Functional importance of alternative proteins is strongly supported by significant evolutionary conservation in vertebrates, invertebrates, and yeast. Our results imply that coding of multiple proteins in a single gene by the use of AltORFs may be a common feature in eukaryotes, and confirm that translation of unconventional ORFs generates an as yet unexplored proteome.</p></div>"}
  • {"files"=>["http://files.figshare.com/1151389/Figure_1.tif"], "pos_in_sequence"=>0, "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "users"=>["Guillaume Tremblay", "Xavier Roucou", "Benoît Vanderperre", "Cyntia Bissonnette", "Solène Vanderperre", "François-Michel Boisvert", "Michel Salzet", "Jean-François Lucier", "Maxence Wisztorski", "Julie Motard"], "links"=>[], "tags"=>["proteins", "proteome", "Proteomic databases", "gene expression", "Protein classes", "Plasma proteins", "protein synthesis", "Protein translation", "Information technology", "databases", "genetics", "Spectrometric identification of proteins", "Molecular cell biology", "Biochemistry", "altorfs", "proteomics"], "title"=>"<p>A database to predict AltORFs in human mRNAs.</p>", "figshare_url"=>"http://figshare.com/articles/_A_database_to_predict_AltORFs_in_human_mRNAs_/770909", "defined_type"=>1, "doi"=>["http://dx.doi.org/10.1371/journal.pone.0070698.g001"], "published_date"=>"2013-08-12 07:01:01", "article_id"=>770909, "categories"=>["Biological Sciences", "Information And Computing Sciences"], "description"=>"<p>(<i>A</i>) A canonical mRNA and its possible AltORFs. The RefORF is the main protein coding ORF annotated in current nucleotide databases. An AltORF is a nucleotide region comprised between an AUG codon and a stop codon distinct from the RefORF and is predicted to encode an alternative protein. AltORFs may be localized in 5′ UTRs, overlapping the 5′UTR and the RefORF, overlapping the RefORF, overlapping the RefORF and the 3′UTR, or in the 3′UTR. (<i>B</i>) Representation of the database generation process. Distinct AltORFs number indicates the total number of predicted AltORFs that encode alternative proteins with unique amino acid sequences. Since an AltORF may be present in several transcripts, the total number of AltORFs in the transcriptome exceeds the number of distinct AltORFs. (<i>C</i>) Distribution in % of AltORFs. (<i>D,E</i>) Distribution of the number of predicted AltORFs per mRNA in (<i>D</i>) and the size distribution of AltORFs (empty bars, left and bottom scale) compared to RefORFs (grey bars, right and top scale) in (<i>E</i>). Boxes and arrows indicate the median.</p>"}
  • {"files"=>["http://files.figshare.com/1151390/Figure_2.tif"], "pos_in_sequence"=>0, "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "users"=>["Guillaume Tremblay", "Xavier Roucou", "Benoît Vanderperre", "Cyntia Bissonnette", "Solène Vanderperre", "François-Michel Boisvert", "Michel Salzet", "Jean-François Lucier", "Maxence Wisztorski", "Julie Motard"], "links"=>[], "tags"=>["proteins", "proteome", "genetics", "Protein classes", "Plasma proteins", "protein synthesis", "Protein translation", "Information technology", "databases", "cultured", "Spectrometric identification of proteins", "gene expression", "Molecular cell biology", "Biochemistry", "Proteomic databases", "proteomics"], "title"=>"<p>Endogenous expression of alternative proteins in cultured cells.</p>", "figshare_url"=>"http://figshare.com/articles/_Endogenous_expression_of_alternative_proteins_in_cultured_cells_/770910", "defined_type"=>1, "doi"=>["http://dx.doi.org/10.1371/journal.pone.0070698.g002"], "published_date"=>"2013-08-12 07:01:01", "article_id"=>770910, "categories"=>["Biological Sciences", "Information And Computing Sciences"], "description"=>"<p>(<i>A</i>) Alternative proteins expression was analyzed by LC-MS/MS in HeLa cells, and a schematic distribution of AltORFs in absolute numbers is shown. There were a total of 129 identified alternative proteins (indicated in the center). (<i>B</i>) Average peptide intensity plot of both the reference and alternative proteins that were co-expressed from 42 genes. (<i>C</i>) Same as (<i>A</i>) with colon cell lines. (<i>D</i>) Venn diagram showing the number of alternative proteins identified in HeLa cells and colon cell lines. The overlap identifies a common list of 14 alternative proteins.</p>"}
  • {"files"=>["http://files.figshare.com/1151391/Figure_3.tif"], "pos_in_sequence"=>0, "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "users"=>["Guillaume Tremblay", "Xavier Roucou", "Benoît Vanderperre", "Cyntia Bissonnette", "Solène Vanderperre", "François-Michel Boisvert", "Michel Salzet", "Jean-François Lucier", "Maxence Wisztorski", "Julie Motard"], "links"=>[], "tags"=>["proteomics", "proteins", "validate", "proteome", "gene expression", "constructs", "Protein classes", "Plasma proteins", "protein synthesis", "Protein translation", "Information technology", "databases", "genetics", "initiation", "Spectrometric identification of proteins", "Molecular cell biology", "Biochemistry", "Proteomic databases", "tagged"], "title"=>"<p>Transfection of tagged constructs validate the expression and translation initiation site prediction of alternative proteins detected by LC-MS/MS.</p>", "figshare_url"=>"http://figshare.com/articles/_Transfection_of_tagged_constructs_validate_the_expression_and_translation_initiation_site_prediction_of_alternative_proteins_detected_by_LC_MS_MS_/770911", "defined_type"=>1, "doi"=>["http://dx.doi.org/10.1371/journal.pone.0070698.g003"], "published_date"=>"2013-08-12 07:01:01", "article_id"=>770911, "categories"=>["Biological Sciences", "Information And Computing Sciences"], "description"=>"<p>Top diagrams represent the constructs used to detect the co-expression of HA-tagged reference and GFP-tagged alternative proteins by western blot analyses of HeLa cell lysates. GFP is inserted before the alternative stop codon in frame with the AltORF. The black line represents a specific region of the endogenous mRNA. For AltORFs located in 5′UTRs of <i>ZNF83</i> and <i>SLC35A4</i>, the constructs do not contain the RefORF since the insertion of GFP may prevent the expression of the downstream RefORF. For AltORFs overlapping the 5′UTR and the RefORF (<i>IDH3B</i>), and for AltORFs overlapping the RefORF (<i>BDH2</i>, <i>NIPA1</i>, <i>SCARB2</i>), the HA tag was introduced before the GFP tag in frame with the RefORFs. Western blots show the co-expression of reference and corresponding alternative proteins in cell lysates with anti-HA and anti-GFP antibodies, respectively. The left and right lanes are cell lysates from cells expressing a construct with a normal alternative initiation AUG codon or with an inactivated alternative initiation AAG codon, respectively. NPTII, encoded in the expression plasmid, was used as a transfection control. Molecular weight markers in kDa are indicated on the right. Bottom panels show confocal/DIC images with the various cellular distributions of GFP-tagged alternative proteins. Nuclei were stained with Hoechst. Scale bar: 10 μm. * The reference protein was not detected due to the small size (<3 kDa) of the truncated HA-tagged reference IDH3B protein.</p>"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"28", "full-text"=>"30", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"22", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2013", "month"=>"8"}
  • {"unique-ip"=>"58", "full-text"=>"53", "pdf"=>"31", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"39", "cited-by"=>"0", "year"=>"2013", "month"=>"9"}
  • {"unique-ip"=>"37", "full-text"=>"30", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"41", "supp-data"=>"21", "cited-by"=>"0", "year"=>"2013", "month"=>"10"}
  • {"unique-ip"=>"29", "full-text"=>"28", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2013", "month"=>"11"}
  • {"unique-ip"=>"23", "full-text"=>"23", "pdf"=>"10", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"75", "full-text"=>"82", "pdf"=>"18", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"30", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"45", "full-text"=>"53", "pdf"=>"17", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"23", "supp-data"=>"30", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"65", "full-text"=>"62", "pdf"=>"48", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"24", "supp-data"=>"14", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"49", "full-text"=>"103", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"34", "full-text"=>"50", "pdf"=>"6", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"74", "full-text"=>"84", "pdf"=>"18", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"24", "full-text"=>"36", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"10", "cited-by"=>"1", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"40", "full-text"=>"47", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"19", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"31", "full-text"=>"37", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"33", "full-text"=>"43", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"27", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"34", "full-text"=>"42", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"15", "cited-by"=>"2", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"35", "full-text"=>"31", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"22", "supp-data"=>"17", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"26", "full-text"=>"24", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"25", "full-text"=>"38", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"31", "full-text"=>"37", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"24", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"46", "full-text"=>"55", "pdf"=>"12", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"42", "full-text"=>"45", "pdf"=>"8", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"59", "full-text"=>"46", "pdf"=>"16", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"57", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"57", "full-text"=>"42", "pdf"=>"9", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"22", "full-text"=>"28", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"28", "supp-data"=>"30", "cited-by"=>"1", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"30", "full-text"=>"32", "pdf"=>"9", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"19", "full-text"=>"16", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"21", "full-text"=>"25", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"24", "full-text"=>"27", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"28", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"26", "full-text"=>"21", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"19", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"19", "full-text"=>"24", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"25", "full-text"=>"29", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"19", "full-text"=>"15", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"17", "full-text"=>"18", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"18", "cited-by"=>"2", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"19", "full-text"=>"16", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"24", "full-text"=>"22", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"3", "cited-by"=>"1", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"17", "full-text"=>"17", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"30", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"21", "full-text"=>"24", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"17", "full-text"=>"15", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"20", "full-text"=>"20", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"18", "full-text"=>"21", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"16", "full-text"=>"18", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"18", "full-text"=>"18", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"14", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"11", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"16", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"23", "full-text"=>"25", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"31", "full-text"=>"35", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"3", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"29", "full-text"=>"39", "pdf"=>"7", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"24", "full-text"=>"22", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"8", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"24", "full-text"=>"28", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"42", "full-text"=>"44", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"38", "full-text"=>"42", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"20", "full-text"=>"21", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"9", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"27", "full-text"=>"26", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"23", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"32", "full-text"=>"34", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"14", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"21", "full-text"=>"21", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"25", "full-text"=>"31", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"31", "full-text"=>"28", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"26", "full-text"=>"26", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"29", "full-text"=>"39", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"5", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[269, 466, 588, 697, 800, 896, 988, 1076, 1165, 1254, 1340, 1417]}, {"subject_area"=>"/Biology and life sciences/Anatomy", "average_usage"=>[248, 440, 562, 666, 765, 856, 944, 1028, 1112, 1198, 1282, 1362, 1430]}, {"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[266, 468, 593, 703, 804, 903, 993, 1084, 1171, 1256, 1339, 1422, 1492]}, {"subject_area"=>"/Biology and life sciences/Computational biology", "average_usage"=>[295, 511, 651, 775, 882, 992, 1100, 1201, 1304, 1400, 1486, 1570, 1650]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[284, 491, 620, 738, 843, 945, 1043, 1137, 1225, 1315, 1400, 1479, 1555]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[272, 466, 589, 702, 806, 903, 995, 1086, 1176, 1258, 1347, 1422, 1493]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[264, 460, 584, 692, 794, 887, 978, 1067, 1154, 1241, 1328, 1408, 1474]}, {"subject_area"=>"/Medicine and health sciences/Anatomy", "average_usage"=>[248, 441, 564, 668, 769, 859, 948, 1034, 1117, 1202, 1290, 1368, 1437]}]}

F1000Prime | Further Information

Loading … Spinner
There are currently no alerts