Antifreeze Protein Prolongs the Life-Time of Insulinoma Cells during Hypothermic Preservation
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{"title"=>"Antifreeze Protein Prolongs the Life-Time of Insulinoma Cells during Hypothermic Preservation", "type"=>"journal", "authors"=>[{"first_name"=>"Tatsuro", "last_name"=>"Kamijima", "scopus_author_id"=>"24470741400"}, {"first_name"=>"Mami", "last_name"=>"Sakashita", "scopus_author_id"=>"57198054795"}, {"first_name"=>"Ai", "last_name"=>"Miura", "scopus_author_id"=>"7202880379"}, {"first_name"=>"Yoshiyuki", "last_name"=>"Nishimiya", "scopus_author_id"=>"6603555390"}, {"first_name"=>"Sakae", "last_name"=>"Tsuda", "scopus_author_id"=>"16147666700"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84884221488", "pui"=>"369833929", "doi"=>"10.1371/journal.pone.0073643", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "sgr"=>"84884221488", "pmid"=>"24069217"}, "id"=>"70824f7e-9499-3a4f-b9eb-e7d51dad45fb", "abstract"=>"It is sometimes desirable to preserve mammalian cells by hypothermia rather than freezing during short term transplantation. Here we found an ability of hypothermic (+4°C) preservation of fish antifreeze protein (AFP) against rat insulinoma cells denoted as RIN-5F. The preservation ability was compared between type I-III AFPs and antifreeze glycoprotein (AFGP), which could be recently mass-prepared by a developed technique utilizing the muscle homogenates, but not the blood serum, of cold-adapted fishes. For AFGP, whose molecular weight is distributed in the range from 2.6 to 34 kDa, only the proteins less than 10 kDa were examined. The viability rate was evaluated by counting of the preserved RIN-5F cells unstained with trypan blue. Significantly, either AFPI or AFPIII dissolved into Euro-Collins (EC) solution at a concentration of 10 mg/ml could preserve approximately 60% of the cells for 5 days at +4°C. The 5-day preserved RIN-5F cells retained the ability to secrete insulin. Only 2% of the cells were, however, preserved for 5 days without AFP. Confocal photomicroscopy experiments further showed the significant binding ability of AFP to the cell surface. These results suggest that fish AFP enables 5-day quality storage of the insulinoma cells collected from a donor without freezing.", "link"=>"http://www.mendeley.com/research/antifreeze-protein-prolongs-lifetime-insulinoma-cells-during-hypothermic-preservation", "reader_count"=>25, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Student > Doctoral Student"=>2, "Researcher"=>2, "Student > Ph. D. Student"=>2, "Student > Master"=>10, "Student > Bachelor"=>5, "Lecturer > Senior Lecturer"=>1, "Professor"=>2}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Student > Doctoral Student"=>2, "Researcher"=>2, "Student > Ph. D. Student"=>2, "Student > Master"=>10, "Student > Bachelor"=>5, "Lecturer > Senior Lecturer"=>1, "Professor"=>2}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>3, "Materials Science"=>1, "Agricultural and Biological Sciences"=>11, "Medicine and Dentistry"=>3, "Chemistry"=>4, "Psychology"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Chemistry"=>{"Chemistry"=>4}, "Psychology"=>{"Psychology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>11}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1206792"], "description"=>"<p>A. Preparation of RIN-5F cells before starting 1–5-day preservation experiments at +4°C. B. Procedures to estimate the number of living cells using the hemocytometer. C. Procedures to measure the amount of insulin secreted from the living cells. Medium-A consists of RPMI-1640 medium containing 10% of fetal bovine serum (FBS). Medium-B consists of the medium containing 1% of FBS. Trypsin-A consists of 0.25% bovine trypsin plus 1 mM of EDTA dissolved in phosphate buffered saline (PBS).</p>", "links"=>[], "tags"=>["rin-5f", "cells"], "article_id"=>801237, "categories"=>["Biological Sciences"], "users"=>["Tatsuro Kamijima", "Mami Sakashita", "Ai Miura", "Yoshiyuki Nishimiya", "Sakae Tsuda"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0073643.g001", "stats"=>{"downloads"=>2, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Flow_charts_showing_the_preparation_of_RIN_5F_cells_and_their_survival_rate_evaluation_/801237", "title"=>"Flow-charts showing the preparation of RIN-5F cells and their survival rate evaluation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-17 01:33:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/1206798"], "description"=>"<p>The images A and B show the slice data of 1 µm-width of HepG2, and A’ and B’ an intact cell images synthesized by stacking of the slices. These 4 images were reproduced from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0073643#pone.0073643-Tsuda1\" target=\"_blank\">[22]</a> with permission. The images C and D are the slice data for RIN-5F cells. All the images were captured after 1 h-preservation at 37°C. Accumulation of AFPIII on the cell-surface is more evident compared with BSA.</p>", "links"=>[], "tags"=>["photomicroscope", "images", "hepatoma", "cells", "insulinoma", "euro", "collins-solutions", "containing", "bsa", "afpiii", "afpi", "labeled"], "article_id"=>801243, "categories"=>["Biological Sciences"], "users"=>["Tatsuro Kamijima", "Mami Sakashita", "Ai Miura", "Yoshiyuki Nishimiya", "Sakae Tsuda"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0073643.g004", "stats"=>{"downloads"=>1, "page_views"=>124, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Confocal_photomicroscope_images_of_human_hepatoma_cells_HepG2_and_rat_insulinoma_cells_RIN_5F_in_Euro_Collins_solutions_containing_0_4_mg_ml_of_BSA_A_AFPIII_B_and_C_and_AFPI_D_labeled_with_fluorescence_/801243", "title"=>"Confocal photomicroscope images of human hepatoma cells (HepG2) and rat insulinoma cells (RIN-5F) in Euro Collins-solutions containing 0.4 mg/ml of BSA (A), AFPIII (B and C), and AFPI (D) labeled with fluorescence.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-17 01:33:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/1206796"], "description"=>"<p>A. The survival rate evaluated by counting the number of living cells (Fig. 1B). B. The survival rate evaluated by measuring the amount of insulin secreted from living cells (Fig. 1C). A freshly made Euro-Collins solution (EC) was used as a basic cell-preservation fluid to dissolve AFPI–III, trehalose, and BSA.</p>", "links"=>[], "tags"=>["insulinoma", "hypothermic"], "article_id"=>801241, "categories"=>["Biological Sciences"], "users"=>["Tatsuro Kamijima", "Mami Sakashita", "Ai Miura", "Yoshiyuki Nishimiya", "Sakae Tsuda"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0073643.g003", "stats"=>{"downloads"=>2, "page_views"=>36, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Time_dependent_change_in_the_survival_rate_of_rat_insulinoma_cell_line_RIN_5F_under_hypothermic_exposure_4_176_C_/801241", "title"=>"Time-dependent change in the survival rate (%) of rat insulinoma cell-line, RIN-5F, under hypothermic exposure (+4°C).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-17 01:33:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/1206794"], "description"=>"<p>A. Electrophoretogram of AFPI–III and AFGP samples provided by Nichirei Foods Inc. on 16.5% tricine-SDS-PAGE. The AFGP sample only contains molecules less than 10 kDa owing to a filtration step. B. The shape of an ice crystal and TH activity obtained for each AFP sample dissolved into the Euro-Collins solution. All protein concentrations were 10 mg/ml.</p>", "links"=>[], "tags"=>["purity", "afp"], "article_id"=>801239, "categories"=>["Biological Sciences"], "users"=>["Tatsuro Kamijima", "Mami Sakashita", "Ai Miura", "Yoshiyuki Nishimiya", "Sakae Tsuda"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0073643.g002", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Analyses_of_purity_and_structural_integrity_of_the_AFP_samples_/801239", "title"=>"Analyses of purity and structural integrity of the AFP samples.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-17 01:33:52"}

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Relative Metric

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