Identification and Structural Characterization of Interneurons of the Drosophila Brain by Monoclonal Antibodies of the Würzburg Hybridoma Library
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{"title"=>"Identification and Structural Characterization of Interneurons of the Drosophila Brain by Monoclonal Antibodies of the Würzburg Hybridoma Library", "type"=>"journal", "authors"=>[{"first_name"=>"Beatriz Blanco", "last_name"=>"Redondo", "scopus_author_id"=>"55856775300"}, {"first_name"=>"Melanie", "last_name"=>"Bunz", "scopus_author_id"=>"55857574900"}, {"first_name"=>"Partho", "last_name"=>"Halder", "scopus_author_id"=>"35336886100"}, {"first_name"=>"Madhumala K.", "last_name"=>"Sadanandappa", "scopus_author_id"=>"45161729700"}, {"first_name"=>"Barbara", "last_name"=>"Mühlbauer", "scopus_author_id"=>"55882129600"}, {"first_name"=>"Felix", "last_name"=>"Erwin", "scopus_author_id"=>"55857406300"}, {"first_name"=>"Alois", "last_name"=>"Hofbauer", "scopus_author_id"=>"7003330885"}, {"first_name"=>"Veronica", "last_name"=>"Rodrigues", "scopus_author_id"=>"7101600459"}, {"first_name"=>"K.", "last_name"=>"VijayRaghavan", "scopus_author_id"=>"7004031702"}, {"first_name"=>"Mani", "last_name"=>"Ramaswami", "scopus_author_id"=>"36834301600"}, {"first_name"=>"Dirk", "last_name"=>"Rieger", "scopus_author_id"=>"7006439900"}, {"first_name"=>"Christian", "last_name"=>"Wegener", "scopus_author_id"=>"35610848400"}, {"first_name"=>"Charlotte", "last_name"=>"Förster", "scopus_author_id"=>"35068039900"}, {"first_name"=>"Erich", "last_name"=>"Buchner", "scopus_author_id"=>"55294764000"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "pui"=>"369833844", "doi"=>"10.1371/journal.pone.0075420", "sgr"=>"84884248232", "scopus"=>"2-s2.0-84884248232", "pmid"=>"24069413"}, "id"=>"9df2bd95-398d-319d-ad06-2c491ec5bd45", "abstract"=>"Several novel synaptic proteins have been identified by monoclonal antibodies (mAbs) of the Würzburg hybridoma library generated against homogenized Drosophila brains, e.g. cysteine string protein, synapse-associated protein of 47 kDa, and Bruchpilot. However, at present no routine technique exists to identify the antigens of mAbs of our library that label only a small number of cells in the brain. Yet these antibodies can be used to reproducibly label and thereby identify these cells by immunohistochemical staining. Here we describe the staining patterns in the Drosophila brain for ten mAbs of the Würzburg hybridoma library. Besides revealing the neuroanatomical structure and distribution of ten different sets of cells we compare the staining patterns with those of antibodies against known antigens and GFP expression patterns driven by selected Gal4 lines employing regulatory sequences of neuronal genes. We present examples where our antibodies apparently stain the same cells in different Gal4 lines suggesting that the corresponding regulatory sequences can be exploited by the split-Gal4 technique for transgene expression exclusively in these cells. The detection of Gal4 expression in cells labeled by mAbs may also help in the identification of the antigens recognized by the antibodies which then in addition to their value for neuroanatomy will represent important tools for the characterization of the antigens. Implications and future strategies for the identification of the antigens are discussed.", "link"=>"http://www.mendeley.com/research/identification-structural-characterization-interneurons-drosophila-brain-monoclonal-antibodies-w%C3%BCrzb", "reader_count"=>24, "reader_count_by_academic_status"=>{"Researcher"=>8, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>4, "Student > Master"=>4, "Student > Bachelor"=>2, "Other"=>1}, "reader_count_by_user_role"=>{"Researcher"=>8, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>4, "Student > Master"=>4, "Student > Bachelor"=>2, "Other"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>16, "Medicine and Dentistry"=>1, "Neuroscience"=>3, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Chemical Engineering"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Neuroscience"=>{"Neuroscience"=>3}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>16}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "Chemical Engineering"=>{"Chemical Engineering"=>1}}, "reader_count_by_country"=>{"France"=>1}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1207218"], "description"=>"<p>Note in (H) that the bright spot at the left rim of the projection is due to a staining artefact from outside the brain. Scale bar 100 µm.</p>", "links"=>[], "tags"=>["confocal", "stacks", "whole-mount", "brains", "stained", "monoclonal", "antibodies", "hybridoma"], "article_id"=>801568, "categories"=>["Biological Sciences"], "users"=>["Beatriz Blanco Redondo", "Melanie Bunz", "Partho Halder", "Madhumala K. Sadanandappa", "Barbara Mühlbauer", "Felix Erwin", "Alois Hofbauer", "Veronica Rodrigues", "K. Vijayraghavan", "Mani Ramaswami", "Dirk Rieger", "Christian Wegener", "Charlotte Förster", "Erich Buchner"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0075420.g001", "stats"=>{"downloads"=>3, "page_views"=>57, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Z_projections_of_confocal_stacks_of_whole_mount_adult_Drosophila_brains_frontal_view_stained_with_ten_different_monoclonal_antibodies_of_the_W_rzburg_hybridoma_library_/801568", "title"=>"Z-projections of confocal stacks of whole-mount adult <i>Drosophila</i> brains (frontal view) stained with ten different monoclonal antibodies of the Würzburg hybridoma library.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-17 01:58:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1207219"], "description"=>"<p>Larval staining of nb169 is shown in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0075420#pone-0075420-g004\" target=\"_blank\">Figure 4K-M</a>, the other seven antibodies did not produce reliable staining in the larval nervous system. Scale bar 100 µm.</p>", "links"=>[], "tags"=>["confocal", "stacks", "larval", "systems", "stained", "monoclonal", "antibodies"], "article_id"=>801569, "categories"=>["Biological Sciences"], "users"=>["Beatriz Blanco Redondo", "Melanie Bunz", "Partho Halder", "Madhumala K. Sadanandappa", "Barbara Mühlbauer", "Felix Erwin", "Alois Hofbauer", "Veronica Rodrigues", "K. Vijayraghavan", "Mani Ramaswami", "Dirk Rieger", "Christian Wegener", "Charlotte Förster", "Erich Buchner"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0075420.g002", "stats"=>{"downloads"=>1, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Z_projections_of_confocal_stacks_of_larval_nervous_systems_stained_with_two_of_the_monoclonal_antibodies_shown_in_Figure_1_/801569", "title"=>"Z-projections of confocal stacks of larval nervous systems stained with two of the monoclonal antibodies shown in Figure 1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-17 01:58:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1207220"], "description"=>"<p>The perfect co-localization in A3 indicates that nb168 could recognize <i>Drosophila</i> PDF or its precursor protein, whereas co-localization in B3 is restricted to the two very large cells stained by nb169 in the posterior protocerebrum. Scale bar 100 µm.</p>", "links"=>[], "tags"=>["confocal", "stacks", "whole-mount", "brains", "double-stained", "mab", "nb168", "anti-pdh", "antiserum", "nb169", "anti-gfp", "expressing", "gfp", "driven", "janelia", "gal4", "r49c04"], "article_id"=>801570, "categories"=>["Biological Sciences"], "users"=>["Beatriz Blanco Redondo", "Melanie Bunz", "Partho Halder", "Madhumala K. Sadanandappa", "Barbara Mühlbauer", "Felix Erwin", "Alois Hofbauer", "Veronica Rodrigues", "K. Vijayraghavan", "Mani Ramaswami", "Dirk Rieger", "Christian Wegener", "Charlotte Förster", "Erich Buchner"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0075420.g003", "stats"=>{"downloads"=>20, "page_views"=>397, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Z_projections_of_confocal_stacks_of_whole_mount_brains_double_stained_with_mAb_nb168_and_anti_PDH_antiserum_A1_3_or_mAb_nb169_and_anti_GFP_in_a_fly_expressing_GFP_driven_by_Janelia_Gal4_line_R49C04_B1_3_/801570", "title"=>"Z-projections of confocal stacks of whole-mount brains double-stained with mAb nb168 and anti-PDH antiserum (A1-3) or mAb nb169 and anti-GFP in a fly expressing GFP driven by Janelia Gal4 line R49C04 (B1-3).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-17 01:58:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1207221"], "description"=>"<p>(A-D) The horizontal cryo-sections stained with mAb nb169 and DAB development show the cell bodies in the lateral protocerebrum at the dorso-ventral level of the fan-shaped and ellipsoid bodies (A), the giant neurons in the caudal cellular cortex with their main neurite (in an adjacent section) at the level of the noduli (B), and the cell bodies innervating the accessory medulla, a horizontal layer of the medulla, and a fiber extending to and branching with varicosities in the lamina cortex (C, D). (E-G) Horizontal cryo-sections double-stained with nb169 (red) and anti-serotonin antiserum (green) demonstrate that serotonergic neurons and cells that contain the nb169 antigen are distinct but that their neurites in the lamina cortex are closely apposed. (H-J) GFP (green) expressed under the control of the Janelia Gal4 line R33G11 co-localizes with the nb169 antigen (red) in frontal confocal optical section (H, I1-3) and in horizontal cryo-section (J1-3). (K-M) In the larval nervous system the nb169 antigen (red) is localized in 10 cells (K, L) of which at least 8 also express GFP (green) under the control of R33G11-Gal4 (M, enlargement of K). Scale bars in A-F, H, K-M: 50 µm; in G, I, J: 10 µm.</p>", "links"=>[], "tags"=>["details", "mab"], "article_id"=>801571, "categories"=>["Biological Sciences"], "users"=>["Beatriz Blanco Redondo", "Melanie Bunz", "Partho Halder", "Madhumala K. Sadanandappa", "Barbara Mühlbauer", "Felix Erwin", "Alois Hofbauer", "Veronica Rodrigues", "K. Vijayraghavan", "Mani Ramaswami", "Dirk Rieger", "Christian Wegener", "Charlotte Förster", "Erich Buchner"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0075420.g004", "stats"=>{"downloads"=>3, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Staining_details_for_mAb_nb169_/801571", "title"=>"Staining details for mAb nb169.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-17 01:58:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1207222"], "description"=>"<p>Due to the variability of cell body positions in different individuals it is only suggestive that the Gal4 expression patterns overlap. Scale bar 100 µm.</p>", "links"=>[], "tags"=>["co-localization", "staining", "mabs", "ab47", "nc24", "gfp", "driven", "janelia", "gal4"], "article_id"=>801572, "categories"=>["Biological Sciences"], "users"=>["Beatriz Blanco Redondo", "Melanie Bunz", "Partho Halder", "Madhumala K. Sadanandappa", "Barbara Mühlbauer", "Felix Erwin", "Alois Hofbauer", "Veronica Rodrigues", "K. Vijayraghavan", "Mani Ramaswami", "Dirk Rieger", "Christian Wegener", "Charlotte Förster", "Erich Buchner"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0075420.g005", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Examples_for_the_co_localization_of_staining_by_mAbs_ab47_A_C_and_nc24_D_F_and_GFP_driven_by_selected_Janelia_Gal4_lines_/801572", "title"=>"Examples for the co-localization of staining by mAbs ab47 (A-C) and nc24 (D-F) and GFP driven by selected Janelia Gal4 lines.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-17 01:58:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1207223"], "description"=>"<p>(A-E) The cell stained by nb169 (red) (A, enlarged in B), and the giant cells in the caudal protocerebrum (C and D from different sections) all are positive for GFP (green) expressed under the control of 386Y amon-Gal4 which has been shown to selectively mark neuropeptide-containing cells. (E) The neurite of a 386Y <i>amon</i>-Gal4>GFP-positive cell at the boundary between lamina cortex and neuropil apparently contains in discrete varicosities the nb169 antigen. (F,G) Examples are shown for co-localization of 386Y <i>amon</i>-Gal4-driven GFP and ca8 antigen in cells of the lateral protocerebrum. (H) Cells containing ab158 antigen in the pars intercerebralis, a region containing many neurosecretory cells, are also positive for amon-Gal4-driven GFP. Scale bar in A: 50 µm; in B-H: 10 µm.</p>", "links"=>[], "tags"=>["antigens", "mabs", "ab158"], "article_id"=>801573, "categories"=>["Biological Sciences"], "users"=>["Beatriz Blanco Redondo", "Melanie Bunz", "Partho Halder", "Madhumala K. Sadanandappa", "Barbara Mühlbauer", "Felix Erwin", "Alois Hofbauer", "Veronica Rodrigues", "K. Vijayraghavan", "Mani Ramaswami", "Dirk Rieger", "Christian Wegener", "Charlotte Förster", "Erich Buchner"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0075420.g006", "stats"=>{"downloads"=>4, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_antigens_detected_by_mAbs_nb169_ca8_and_ab158_may_be_related_to_neuropeptides_/801573", "title"=>"The antigens detected by mAbs nb169, ca8, and ab158 may be related to neuropeptides.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-17 01:58:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1207224"], "description"=>"<p>List of mAbs compared with GFP (detected by anti-GFP (1∶1000) driven by Gal4-lines) or stainings with antibodies against known antigens (pigment dispersing hormone, PDH (1∶1500); myoinhibitory peptide, MIP (1∶1000); neuropeptide F, NPF (1∶300); period protein, PER (1∶2000); serotonin, 5HT (1∶400)).</p>", "links"=>[], "tags"=>["mabs", "compared", "gfp", "anti-gfp", "driven", "stainings", "antibodies", "antigens", "dispersing", "pdh", "myoinhibitory", "mip", "neuropeptide", "npf", "5ht"], "article_id"=>801574, "categories"=>["Biological Sciences"], "users"=>["Beatriz Blanco Redondo", "Melanie Bunz", "Partho Halder", "Madhumala K. Sadanandappa", "Barbara Mühlbauer", "Felix Erwin", "Alois Hofbauer", "Veronica Rodrigues", "K. Vijayraghavan", "Mani Ramaswami", "Dirk Rieger", "Christian Wegener", "Charlotte Förster", "Erich Buchner"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0075420.t001", "stats"=>{"downloads"=>7, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_List_of_mAbs_compared_with_GFP_detected_by_anti_GFP_1_8758_1000_driven_by_Gal4_lines_or_stainings_with_antibodies_against_known_antigens_pigment_dispersing_hormone_PDH_1_8758_1500_myoinhibitory_peptide_MIP_1_8758_1000_neuropeptide_F_NPF_1_8758_300_period/801574", "title"=>"List of mAbs compared with GFP (detected by anti-GFP (1∶1000) driven by Gal4-lines) or stainings with antibodies against known antigens (pigment dispersing hormone, PDH (1∶1500); myoinhibitory peptide, MIP (1∶1000); neuropeptide F, NPF (1∶300); period protein, PER (1∶2000); serotonin, 5HT (1∶400)).", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-09-17 01:58:38"}

PMC Usage Stats | Further Information

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Relative Metric

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