Strategies for Detection of Plasmodium species Gametocytes
Publication Date
September 27, 2013
Journal
PLOS ONE
Authors
Rahel Wampfler, Felistas Mwingira, Sarah Javati, Leanne Robinson, et al
Volume
8
Issue
9
Pages
e76316
DOI
https://dx.plos.org/10.1371/journal.pone.0076316
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0076316
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/24312682
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3848260
Europe PMC
http://europepmc.org/abstract/MED/24312682
Web of Science
000325223900097
Scopus
84884693697
Mendeley
http://www.mendeley.com/research/strategies-detection-plasmodium-species-gametocytes-1
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Mendeley | Further Information

{"title"=>"Strategies for Detection of Plasmodium species Gametocytes", "type"=>"journal", "authors"=>[{"first_name"=>"Rahel", "last_name"=>"Wampfler", "scopus_author_id"=>"55865803500"}, {"first_name"=>"Felistas", "last_name"=>"Mwingira", "scopus_author_id"=>"37261651100"}, {"first_name"=>"Sarah", "last_name"=>"Javati", "scopus_author_id"=>"55866229300"}, {"first_name"=>"Leanne", "last_name"=>"Robinson", "scopus_author_id"=>"55139326100"}, {"first_name"=>"Inoni", "last_name"=>"Betuela", "scopus_author_id"=>"6507748085"}, {"first_name"=>"Peter", "last_name"=>"Siba", "scopus_author_id"=>"12241224200"}, {"first_name"=>"Hans Peter", "last_name"=>"Beck", "scopus_author_id"=>"26643163500"}, {"first_name"=>"Ivo", "last_name"=>"Mueller", "scopus_author_id"=>"7006910183"}, {"first_name"=>"Ingrid", "last_name"=>"Felger", "scopus_author_id"=>"7003856190"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84884693697", "pmid"=>"24312682", "isbn"=>"1932-6203", "pui"=>"369903757", "issn"=>"19326203", "scopus"=>"2-s2.0-84884693697", "doi"=>"10.1371/journal.pone.0076316"}, "id"=>"cce77882-9b7a-3778-8750-feffad099860", "abstract"=>"Carriage and density of gametocytes, the transmission stages of malaria parasites, are determined for predicting the infectiousness of humans to mosquitoes. This measure is used for evaluating interventions that aim at reducing malaria transmission. Gametocytes need to be detected by amplification of stage-specific transcripts, which requires RNA-preserving blood sampling. For simultaneous, highly sensitive quantification of both, blood stages and gametocytes, we have compared and optimized different strategies for field and laboratory procedures in a cross sectional survey in 315 5-9 yr old children from Papua New Guinea. qRT-PCR was performed for gametocyte markers pfs25 and pvs25, Plasmodium species prevalence was determined by targeting both, 18S rRNA genes and transcripts. RNA-based parasite detection resulted in a P. falciparum positivity of 24.1%; of these 40.8% carried gametocytes. P. vivax positivity was 38.4%, with 38.0% of these carrying gametocytes. Sensitivity of DNA-based parasite detection was substantially lower with 14.1% for P. falciparum and 19.6% for P. vivax. Using the lower DNA-based prevalence of asexual stages as a denominator increased the percentage of gametocyte-positive infections to 59.1% for P. falciparum and 52.4% for P. vivax. For studies requiring highly sensitive and simultaneous quantification of sexual and asexual parasite stages, 18S rRNA transcript-based detection saves efforts and costs. RNA-based positivity is considerably higher than other methods. On the other hand, DNA-based parasite quantification is robust and permits comparison with other globally generated molecular prevalence data. Molecular monitoring of low density asexual and sexual parasitaemia will support the evaluation of effects of up-scaled antimalarial intervention programs and can also inform about small scale spatial variability in transmission intensity.", "link"=>"http://www.mendeley.com/research/strategies-detection-plasmodium-species-gametocytes-1", "reader_count"=>90, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Librarian"=>1, "Researcher"=>27, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>20, "Student > Postgraduate"=>2, "Student > Master"=>20, "Other"=>1, "Student > Bachelor"=>8, "Lecturer"=>2, "Professor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>1, "Librarian"=>1, "Researcher"=>27, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>20, "Student > Postgraduate"=>2, "Student > Master"=>20, "Other"=>1, "Student > Bachelor"=>8, "Lecturer"=>2, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>4, "Biochemistry, Genetics and Molecular Biology"=>12, "Agricultural and Biological Sciences"=>46, "Medicine and Dentistry"=>17, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Chemistry"=>2, "Computer Science"=>1, "Immunology and Microbiology"=>7}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>17}, "Chemistry"=>{"Chemistry"=>2}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>7}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>46}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>12}, "Unspecified"=>{"Unspecified"=>4}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"United States"=>1, "Tanzania"=>1, "Brazil"=>1, "Italy"=>1, "India"=>1, "Spain"=>1}, "group_count"=>3}

CrossRef

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1218886"], "description"=>"<p>20 samples were chosen to represent a wide range of transcript copy numbers at start of the 2 yr storage period. The initial copy numbers (black bars) are shown next to copy number detected 2 yrs later in the same RNA sample (white bars).</p>", "links"=>[], "tags"=>[], "article_id"=>810398, "categories"=>["Biological Sciences"], "users"=>["Rahel Wampfler", "Felistas Mwingira", "Sarah Javati", "Leanne Robinson", "Inoni Betuela", "Peter Siba", "Hans-Peter Beck", "Ivo Mueller", "Ingrid Felger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0076316.g005", "stats"=>{"downloads"=>0, "page_views"=>56, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_extended_storage_time_on_pfs25_transcripts_/810398", "title"=>"Effect of extended storage time on <i>pfs25</i> transcripts.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-27 02:28:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/1218883"], "description"=>"<p><i>P. falciparum</i> (upper panel) and <i>P. vivax</i> (lower panel). Boxed values indicate the correlation coefficient (r<sup>2</sup>) and the conversion functions extracted from these data. All correlation coefficients (r<sup>2</sup>) were significant (p-value < 0.001). </p>", "links"=>[], "tags"=>["rna-based", "quantification", "parasites", "qpcr", "qrt-pcr", "18s", "rrna", "genes", "transcripts", "microscopy"], "article_id"=>810395, "categories"=>["Biological Sciences"], "users"=>["Rahel Wampfler", "Felistas Mwingira", "Sarah Javati", "Leanne Robinson", "Inoni Betuela", "Peter Siba", "Hans-Peter Beck", "Ivo Mueller", "Ingrid Felger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0076316.g002", "stats"=>{"downloads"=>0, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_DNA_versus_RNA_based_quantification_of_Plasmodium_parasites_by_qPCR_and_qRT_PCR_of_18S_rRNA_genes_or_transcripts_in_comparison_to_light_microscopy_LM_/810395", "title"=>"DNA- versus RNA-based quantification of <i>Plasmodium</i> parasites by qPCR and qRT-PCR of 18S rRNA genes or transcripts in comparison to light microscopy (LM).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-27 02:28:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/1218882"], "description"=>"<p>Red and blue frames indicate assays done on DNA and on RNA, respectively. Orange and green boxes are <i>P. falciparum</i> and <i>P. vivax</i>-specific assays, respectively. <i>P. malariae</i> and <i>P. ovale</i> assays are not included in the diagram.</p>", "links"=>[], "tags"=>["diagram", "molecular", "performed", "detection", "asexual", "parasite", "stages", "samples"], "article_id"=>810394, "categories"=>["Biological Sciences"], "users"=>["Rahel Wampfler", "Felistas Mwingira", "Sarah Javati", "Leanne Robinson", "Inoni Betuela", "Peter Siba", "Hans-Peter Beck", "Ivo Mueller", "Ingrid Felger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0076316.g001", "stats"=>{"downloads"=>1, "page_views"=>88, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Flow_diagram_of_molecular_analyses_performed_for_detection_of_asexual_and_sexual_parasite_stages_of_P_vivax_and_P_falciparum_in_field_samples_from_PNG_/810394", "title"=>"Flow diagram of molecular analyses performed for detection of asexual and sexual parasite stages of <i>P. vivax</i> and <i>P. falciparum</i> in field samples from PNG.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-27 02:28:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/1218885"], "description"=>"<p>Dashed lines indicate 95% confidence interval of intercept.</p>", "links"=>[], "tags"=>["generated", "3d7", "vitro", "converting", "transcript", "numbers", "gametocyte"], "article_id"=>810397, "categories"=>["Biological Sciences"], "users"=>["Rahel Wampfler", "Felistas Mwingira", "Sarah Javati", "Leanne Robinson", "Inoni Betuela", "Peter Siba", "Hans-Peter Beck", "Ivo Mueller", "Ingrid Felger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0076316.g004", "stats"=>{"downloads"=>1, "page_views"=>53, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Gametocyte_trend_line_generated_with_3D7_P_falciparum_in_vitro_culture_for_converting_pfs25_transcript_copy_numbers_into_gametocyte_counts_/810397", "title"=>"Gametocyte trend line generated with 3D7 <i>P. falciparum</i> in vitro culture for converting <i>pfs25</i> transcript copy numbers into gametocyte counts.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-27 02:28:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/1218884"], "description"=>"<p>(A) <i>P. falciparum</i>, (B) <i>P. vivax</i>. Gametocyte positivity (left panel) and transcript copy numbers (right panel) are shown for RNAprotect solution versus filter paper soaked in TRIzol. Only samples were compared for which both measurements were available. </p>", "links"=>[], "tags"=>["sampling", "strategies", "measuring", "gametocyte", "prevalence"], "article_id"=>810396, "categories"=>["Biological Sciences"], "users"=>["Rahel Wampfler", "Felistas Mwingira", "Sarah Javati", "Leanne Robinson", "Inoni Betuela", "Peter Siba", "Hans-Peter Beck", "Ivo Mueller", "Ingrid Felger"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0076316.g003", "stats"=>{"downloads"=>0, "page_views"=>79, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparison_of_two_blood_sampling_strategies_for_measuring_gametocyte_prevalence_rates_/810396", "title"=>"Comparison of two blood sampling strategies for measuring gametocyte prevalence rates.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-09-27 02:28:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/1218887", "https://ndownloader.figshare.com/files/1218888", "https://ndownloader.figshare.com/files/1218889", "https://ndownloader.figshare.com/files/1218890", "https://ndownloader.figshare.com/files/1218891", "https://ndownloader.figshare.com/files/1218892"], "description"=>"<div><p>Carriage and density of gametocytes, the transmission stages of malaria parasites, are determined for predicting the infectiousness of humans to mosquitoes. This measure is used for evaluating interventions that aim at reducing malaria transmission. Gametocytes need to be detected by amplification of stage-specific transcripts, which requires RNA-preserving blood sampling. For simultaneous, highly sensitive quantification of both, blood stages and gametocytes, we have compared and optimized different strategies for field and laboratory procedures in a cross sectional survey in 315 5-9 yr old children from Papua New Guinea. qRT-PCR was performed for gametocyte markers pfs25 and pvs25, <i>Plasmodium</i> species prevalence was determined by targeting both, 18S rRNA genes and transcripts. RNA-based parasite detection resulted in a <i>P. falciparum</i> positivity of 24.1%; of these 40.8% carried gametocytes. <i>P. vivax</i> positivity was 38.4%, with 38.0% of these carrying gametocytes. Sensitivity of DNA-based parasite detection was substantially lower with 14.1% for <i>P. falciparum</i> and 19.6% for <i>P. vivax</i>. Using the lower DNA-based prevalence of asexual stages as a denominator increased the percentage of gametocyte-positive infections to 59.1% for <i>P. falciparum</i> and 52.4% for <i>P. vivax</i>. For studies requiring highly sensitive and simultaneous quantification of sexual and asexual parasite stages, 18S rRNA transcript-based detection saves efforts and costs. RNA-based positivity is considerably higher than other methods. On the other hand, DNA-based parasite quantification is robust and permits comparison with other globally generated molecular prevalence data. Molecular monitoring of low density asexual and sexual parasitaemia will support the evaluation of effects of up-scaled antimalarial intervention programs and can also inform about small scale spatial variability in transmission intensity.</p> </div>", "links"=>[], "tags"=>["strategies", "detection", "gametocytes"], "article_id"=>810399, "categories"=>["Biological Sciences"], "users"=>["Rahel Wampfler", "Felistas Mwingira", "Sarah Javati", "Leanne Robinson", "Inoni Betuela", "Peter Siba", "Hans-Peter Beck", "Ivo Mueller", "Ingrid Felger"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0076316.s001", "https://dx.doi.org/10.1371/journal.pone.0076316.s002", "https://dx.doi.org/10.1371/journal.pone.0076316.s003", "https://dx.doi.org/10.1371/journal.pone.0076316.s004", "https://dx.doi.org/10.1371/journal.pone.0076316.s005", "https://dx.doi.org/10.1371/journal.pone.0076316.s006"], "stats"=>{"downloads"=>25, "page_views"=>186, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Strategies_for_Detection_of_Plasmodium_species_Gametocytes/810399", "title"=>"Strategies for Detection of <i>Plasmodium</i><i> species</i> Gametocytes", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-09-27 02:28:45"}

PMC Usage Stats | Further Information

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Relative Metric

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