Fatty Acids are Key in 4-Hydroxy-2-Nonenal-Mediated Activation of Uncoupling Proteins 1 and 2
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{"title"=>"Fatty Acids are Key in 4-Hydroxy-2-Nonenal-Mediated Activation of Uncoupling Proteins 1 and 2", "type"=>"journal", "authors"=>[{"first_name"=>"Elena A.", "last_name"=>"Malingriaux", "scopus_author_id"=>"55906137200"}, {"first_name"=>"Anne", "last_name"=>"Rupprecht", "scopus_author_id"=>"23571053600"}, {"first_name"=>"Lars", "last_name"=>"Gille", "scopus_author_id"=>"7004440672"}, {"first_name"=>"Olga", "last_name"=>"Jovanovic", "scopus_author_id"=>"57197965284"}, {"first_name"=>"Petr", "last_name"=>"Jezek", "scopus_author_id"=>"7006093271"}, {"first_name"=>"Martin", "last_name"=>"Jaburek", "scopus_author_id"=>"6602561082"}, {"first_name"=>"Elena E.", "last_name"=>"Pohl", "scopus_author_id"=>"7006737024"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"24204965", "sgr"=>"84886626298", "doi"=>"10.1371/journal.pone.0077786", "scopus"=>"2-s2.0-84886626298", "pui"=>"370137490", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "issn"=>"19326203"}, "id"=>"20401c0d-d646-3328-8eb2-62ae5e8bda9b", "abstract"=>"The production of reactive oxygen species (ROS) in mitochondria is very sensitive to the proton motive force and may be decreased by mild uncoupling, mediated e.g. by mitochondrial uncoupling proteins (UCPs). UCPs were conversely hypothesized to be activated by ROS. Conclusions from experiments studying the reactive product of lipid peroxidation 4-hydroxy-2-nonenal (HNE) in isolated mitochondria and UCP knock-out mice are highly controversial. Here we investigated the molecular mechanism of HNE action by evaluating the separate contributions of lipid and protein phases of the membrane and by comparing UCP1 and UCP2, which were reconstituted in planar lipid bilayers. We demonstrated that aldehyde does not directly activate either UCP1 or UCP2. However, HNE strongly potentiated the membrane conductance increase (Gm) mediated by different long-chain fatty acids in UCP-containing and in UCP-free membranes and this suggest the involvement of both lipid-mediated and protein-mediated mechanisms with FA playing the central role. Gm increase was concentration-dependent and exhibited a typical saturation kinetic with the binding constant 0.3 mM. By using Electron Paramagnetic Resonance, membrane fluidity change could be excluded as a cause for the HNE-mediated increase in the presence of FA. The impact of the HNE binding to definite positively charged UCP amino acid residues is discussed as a possible protein-mediated mechanism of the UCP activation.", "link"=>"http://www.mendeley.com/research/fatty-acids-key-4hydroxy2nonenalmediated-activation-uncoupling-proteins-1-2", "reader_count"=>20, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>7, "Student > Ph. D. Student"=>5, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>2, "Professor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>7, "Student > Ph. D. Student"=>5, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>2, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>4, "Medicine and Dentistry"=>1, "Agricultural and Biological Sciences"=>10, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Physics and Astronomy"=>1, "Chemistry"=>1, "Social Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Social Sciences"=>{"Social Sciences"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>10}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}, "Unspecified"=>{"Unspecified"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"Czech Republic"=>1}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1259606"], "description"=>"<p>The concentration of membrane E. coli polar lipid, UCP1 and HNE were 1/ml, 5.5 µg/mg of lipid, and 0.86 mM. Buffer solution contained 50 mM K<sub>2</sub>SO<sub>4</sub>, 25 mM TES, 0.6 mM EGTA at pH 7.4 and T = 32°C. Data points represent mean ± standard deviation from 3–5 independent experiments.</p>", "links"=>[], "tags"=>["membrane", "hne-mediated", "conductance"], "article_id"=>833549, "categories"=>["Biological Sciences"], "users"=>["Elena A. Malingriaux", "Anne Rupprecht", "Lars Gille", "Olga Jovanovic", "Petr Jezek", "Martin Jaburek", "Elena E. Pohl"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0077786.g003", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_membrane_potential_on_the_HNE_mediated_membrane_conductance_in_the_absence_of_AA_/833549", "title"=>"Effect of membrane potential on the HNE-mediated membrane conductance in the absence of AA.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-28 02:58:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/1259605"], "description"=>"<p>Membranes from E. polar lipid were reconstituted with 15% arachidonic acid and 10.5 µg/(mg lipid) UCP1 (charge 19). Buffer solution contained 50 mM Na<sub>2</sub>SO<sub>4</sub>, 10 mM Tris, 10 mM MES, 0,6 mM EGTA, at pH 7.5 and T = 32°C. Data points represent mean ± standard deviation from at least 3 independent experiments. The data points were fitted following Michaelis-Menten kinetics as described in the text.</p>", "links"=>[], "tags"=>["ucp-mediated", "proton", "conductance", "hne"], "article_id"=>833548, "categories"=>["Biological Sciences"], "users"=>["Elena A. Malingriaux", "Anne Rupprecht", "Lars Gille", "Olga Jovanovic", "Petr Jezek", "Martin Jaburek", "Elena E. Pohl"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0077786.g002", "stats"=>{"downloads"=>0, "page_views"=>56, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Dependence_of_UCP_mediated_proton_conductance_on_HNE_concentration_/833548", "title"=>"Dependence of UCP-mediated proton conductance on HNE concentration.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-28 02:58:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/1259609"], "description"=>"<p>Liposomes (5 mg/ml) were made from E.coli lipid. The concentration of the spin label 5-doxyl stearic acid (5-DSA) was 7.5 nmol/(mg lipid). Graph (A) shows a typical EPR spectrum of 5-DSA incorporated in liposomes in the absence and presence of AA. Details of the spectral shift in the low-field and the high-field line caused by AA are shown in (B) and (C), respectively. (D) The order parameter measured in control liposomes (S<sub>0</sub>) was set to 100 and all other order parameters (S<sub>x</sub>) were expressed in relation to this value. S<sub>0</sub> and S<sub>x</sub> were measured with or without additives (AA, HNE, Ethanol).</p>", "links"=>[], "tags"=>["arachidonic", "hydroxynonenal"], "article_id"=>833552, "categories"=>["Biological Sciences"], "users"=>["Elena A. Malingriaux", "Anne Rupprecht", "Lars Gille", "Olga Jovanovic", "Petr Jezek", "Martin Jaburek", "Elena E. Pohl"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0077786.g004", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Alteration_of_order_parameters_in_the_presence_of_arachidonic_acid_AA_and_or_hydroxynonenal_HNE_/833552", "title"=>"Alteration of order parameters in the presence of arachidonic acid (AA) and/or hydroxynonenal (HNE).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-28 02:58:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/1259603"], "description"=>"<p>A. Influence of HNE and/or arachidonic acid (AA) on G in the absence (white bars) and presence of UCP1 (grey bars) or UCP2 (dark grey bars). The concentrations of E. coli polar lipid, UCP1, UCP2 and HNE were 1 mg/ml, 5.5 µg/(mg of lipid, charge 9), 4.7 µg/(mg of lipid) and 0.86 mM respectively. AA was directly added at a concentration of 15 mol% to the lipid phase prior the membrane formation. The buffer solution contained 50 mM K<sub>2</sub>SO<sub>4</sub>, 25 mM TES 0.6 mM EGTA at pH 7.4 and T = 32°C. HNE was directly added to the buffer solution. Data points represent mean ± standard deviation from 3–5 independent experiments. B. Comparison of membrane conductance ratios in the presence (G) and absence (G<sub>0</sub>) of 0.64 mM HNE for arachidic (ArA, 20:0), linoleic (LA, 18:2, ω6), eicosatrienoic (EA, 20:3, ω3) and arachidonic acid (AA, 20:4, ω6). Inset. Membrane conductance in the presence (grey bars) and absence (black bars) of HNE. Membranes from E. polar lipid were reconstituted with 15 mol% FA and 7 µg UCP1/(mg of lipid) (charge 33). The buffer solution contained 50 mM Na<sub>2</sub>SO<sub>4</sub>, 10 mM Tris, 10 mM MES, 0.6 mM EGTA, at pH 7.4 and T = 32°C. Data represent mean ± standard deviation from at least 3 independent experiments.</p>", "links"=>[], "tags"=>["4-hydroxy-2-nonenal", "membrane", "conductance"], "article_id"=>833546, "categories"=>["Biological Sciences"], "users"=>["Elena A. Malingriaux", "Anne Rupprecht", "Lars Gille", "Olga Jovanovic", "Petr Jezek", "Martin Jaburek", "Elena E. Pohl"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0077786.g001", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_effect_of_4_hydroxy_2_nonenal_on_membrane_conductance_G_/833546", "title"=>"The effect of 4-hydroxy-2-nonenal on membrane conductance (G).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-28 02:58:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/1259612"], "description"=>"<p>Position of cysteines (pink or mint), lysines (yellow) and histidines (red) in UCP1 (A) and UCP2 (B), visualized with PyMOL.</p>", "links"=>[], "tags"=>["cysteines", "lysines", "histidines", "ucp1", "ucp2", "visualized"], "article_id"=>833555, "categories"=>["Biological Sciences"], "users"=>["Elena A. Malingriaux", "Anne Rupprecht", "Lars Gille", "Olga Jovanovic", "Petr Jezek", "Martin Jaburek", "Elena E. Pohl"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0077786.g006", "stats"=>{"downloads"=>1, "page_views"=>31, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Position_of_cysteines_pink_or_mint_lysines_yellow_and_histidines_red_in_UCP1_A_and_UCP2_B_visualized_with_PyMOL_/833555", "title"=>"Position of cysteines (pink or mint), lysines (yellow) and histidines (red) in UCP1 (A) and UCP2 (B), visualized with PyMOL.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-28 02:58:18"}
  • {"files"=>["https://ndownloader.figshare.com/files/1259611"], "description"=>"<p>A. Membrane conductance decrease in the presence of Cys, Lys, and His blockers. Membranes were made from E. polar lipid (1mg/ml) and reconstituted with arachidonic acid (15 mol%) and UCP1 (charge 18, 11.9 µg/(mg of lipid). Bar 1 shows the conductance of the membrane without additives (control). NEM, NHS and MNBS in concentrations of 0.5 µM, 1.3 µM and 0.2 µM respectively were incubated with protein as described in Results. HNE was added in concentration 860 µM (grey bars). Buffer solution contained 50 mM Na<sub>2</sub>SO<sub>4</sub>, 10 mM Tris, 10 mM MES, 0.6 mM EGTA at pH 7.5. Data points represent mean ± standard deviation from 3–5 independent experiments. * p<0.05; ** p<0.01. B. Western blot analysis of the UCP1-containing proteoliposomes in the presence of different additives (arachidonic acid, AA; hydroxynonenal, HNE; NEM; NHS; MNBS). Only HNE-modified UCP1 is recognized by HNE antibody.</p>", "links"=>[], "tags"=>["hne-mediated", "ucp1"], "article_id"=>833554, "categories"=>["Biological Sciences"], "users"=>["Elena A. Malingriaux", "Anne Rupprecht", "Lars Gille", "Olga Jovanovic", "Petr Jezek", "Martin Jaburek", "Elena E. Pohl"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0077786.g005", "stats"=>{"downloads"=>1, "page_views"=>32, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Analysis_of_HNE_mediated_UCP1_modification_/833554", "title"=>"Analysis of HNE-mediated UCP1 modification.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-28 02:58:18"}

PMC Usage Stats | Further Information

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  • {"unique-ip"=>"10", "full-text"=>"15", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
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  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
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  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
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  • {"unique-ip"=>"12", "full-text"=>"15", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
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  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
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  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
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Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[269, 466, 588, 697, 800, 896, 988, 1076, 1165, 1254, 1340, 1417]}, {"subject_area"=>"/Medicine and health sciences/Physiology", "average_usage"=>[258, 449, 572, 679, 775, 866, 956, 1041, 1124, 1211, 1291, 1371, 1437]}, {"subject_area"=>"/Physical sciences/Physics", "average_usage"=>[254, 421, 527, 626, 720, 813, 900, 983, 1063, 1136, 1210, 1283, 1342]}]}
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