Transient Receptor Potential Channels Encode Volatile Chemicals Sensed by Rat Trigeminal Ganglion Neurons
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{"title"=>"Transient Receptor Potential Channels Encode Volatile Chemicals Sensed by Rat Trigeminal Ganglion Neurons", "type"=>"journal", "authors"=>[{"first_name"=>"Matthias", "last_name"=>"Lübbert", "scopus_author_id"=>"40561421000"}, {"first_name"=>"Jessica", "last_name"=>"Kyereme", "scopus_author_id"=>"55382448400"}, {"first_name"=>"Nicole", "last_name"=>"Schöbel", "scopus_author_id"=>"23100678500"}, {"first_name"=>"Leopoldo", "last_name"=>"Beltrán", "scopus_author_id"=>"55822278400"}, {"first_name"=>"Christian Horst", "last_name"=>"Wetzel", "scopus_author_id"=>"56020311900"}, {"first_name"=>"Hanns", "last_name"=>"Hatt", "scopus_author_id"=>"7006063364"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84886924302", "pui"=>"370077807", "doi"=>"10.1371/journal.pone.0077998", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "sgr"=>"84886924302", "pmid"=>"24205061"}, "id"=>"b9cc403d-7f28-3348-b65e-21a64c325272", "abstract"=>"Primary sensory afferents of the dorsal root and trigeminal ganglia constantly transmit sensory information depicting the individual's physical and chemical environment to higher brain regions. Beyond the typical trigeminal stimuli (e.g. irritants), environmental stimuli comprise a plethora of volatile chemicals with olfactory components (odorants). In spite of a complete loss of their sense of smell, anosmic patients may retain the ability to roughly discriminate between different volatile compounds. While the detailed mechanisms remain elusive, sensory structures belonging to the trigeminal system seem to be responsible for this phenomenon. In order to gain a better understanding of the mechanisms underlying the activation of the trigeminal system by volatile chemicals, we investigated odorant-induced membrane potential changes in cultured rat trigeminal neurons induced by the odorants vanillin, heliotropyl acetone, helional, and geraniol. We observed the dose-dependent depolarization of trigeminal neurons upon application of these substances occurring in a stimulus-specific manner and could show that distinct neuronal populations respond to different odorants. Using specific antagonists, we found evidence that TRPA1, TRPM8, and/or TRPV1 contribute to the activation. In order to further test this hypothesis, we used recombinantly expressed rat and human variants of these channels to investigate whether they are indeed activated by the odorants tested. We additionally found that the odorants dose-dependently inhibit two-pore potassium channels TASK1 and TASK3 heterologously expressed In Xenopus laevis oocytes. We suggest that the capability of various odorants to activate different TRP channels and to inhibit potassium channels causes neuronal depolarization and activation of distinct subpopulations of trigeminal sensory neurons, forming the basis for a specific representation of volatile chemicals in the trigeminal ganglia.", "link"=>"http://www.mendeley.com/research/transient-receptor-potential-channels-encode-volatile-chemicals-sensed-rat-trigeminal-ganglion-neuro", "reader_count"=>22, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Researcher"=>2, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>4, "Student > Bachelor"=>3}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Researcher"=>2, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>4, "Student > Bachelor"=>3}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>1, "Medicine and Dentistry"=>2, "Agricultural and Biological Sciences"=>13, "Neuroscience"=>1, "Chemistry"=>1, "Social Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Neuroscience"=>{"Neuroscience"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Social Sciences"=>{"Social Sciences"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>13}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Unspecified"=>{"Unspecified"=>2}, "Environmental Science"=>{"Environmental Science"=>1}}, "reader_count_by_country"=>{"Netherlands"=>1, "Germany"=>1}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1253495"], "description"=>"<p><b>A:</b> Bar chart, depicting the responsiveness (%) of TG neurons to the odorants vanillin (1 mM; n = 54), HTPA (1 mM; n = 125), helional (1 mM; n = 121), and geraniol (1 mM; n = 101), as well as to cap (3.3 µM) and men (300 µM). Detailed percentages can be derived from table S1. <b>B:</b> Bar chart, depicting the responsiveness (%) of TG neurons to the odorants vanillin (1 mM; n = 87), HTPA (1 mM; n = 68), helional (1 mM; n = 68), and geraniol (1 mM; n = 69) as well as to cap (3.3 µM) and AITC (50 µM). Detailed percentages can be derived from table S1. <b>(C-E)</b> Box plot diagrams depicting the odorant-evoked membrane potential depolarization in cultured TG neurons during whole-cell CC recordings in the presence and the absence of CPZ (C), BCTC (D) or HC (E). <b>C:</b> Recordings from menthol- and capsaicin-sensitive neurons were subdivided. Depolarizations triggered by vanillin (n = 54), HTPA (n = 70), helional (n = 76), and geraniol (n = 59) in the presence and in the absence of CPZ, as well as percentage values of inhibitions can be derived from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077998#pone-0077998-t004\" target=\"_blank\">table 4</a>. <b>D:</b> Recordings from menthol- and capsaicin-sensitive neurons were subdivided. Depolarizations triggered by HTPA (n = 55), helional (n = 45), and geraniol (n = 42) in the presence and in the absence of BCTC as well as percentage values of inhibitions can be derived from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077998#pone-0077998-t004\" target=\"_blank\">table 4</a>. <b>E:</b> Recordings from AITC/cap- and cap-sensitive neurons were subdivided, respectively (original traces are depicted in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077998#pone.0077998.s002\" target=\"_blank\">fig. S2</a>). Depolarizations triggered by vanillin (n = 87), HTPA (n = 68), helional (n = 68), and geraniol (n = 69) in the presence and in the absence of HC can be derived from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077998#pone-0077998-t004\" target=\"_blank\">table 4</a>.</p>", "links"=>[], "tags"=>["hc", "inhibit", "odorant-evoked", "activations", "cultured", "tg"], "article_id"=>829054, "categories"=>["Biological Sciences"], "users"=>["Matthias Lübbert", "Jessica Kyereme", "Nicole Schöbel", "Leopoldo Beltrán", "Christian Horst Wetzel", "Hanns Hatt"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0077998.g004", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_CPZ_and_HC_inhibit_odorant_evoked_activations_of_cultured_TG_neurons_/829054", "title"=>"CPZ and HC inhibit odorant-evoked activations of cultured TG neurons.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-21 03:00:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/1253506"], "description"=>"<p>Amplitudes of odorant-evoked in- (−100 mV) and outward (+100 mV) currents via functionally expressed rat TRPV1, TRPM8, and TRPA1.</p>", "links"=>[], "tags"=>["odorant-evoked", "in-", "outward", "currents", "functionally"], "article_id"=>829065, "categories"=>["Biological Sciences"], "users"=>["Matthias Lübbert", "Jessica Kyereme", "Nicole Schöbel", "Leopoldo Beltrán", "Christian Horst Wetzel", "Hanns Hatt"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0077998.t005", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Amplitudes_of_odorant_evoked_in_8722_100_mV_and_outward_100_mV_currents_via_functionally_expressed_rat_TRPV1_TRPM8_and_TRPA1_/829065", "title"=>"Amplitudes of odorant-evoked in- (−100 mV) and outward (+100 mV) currents via functionally expressed rat TRPV1, TRPM8, and TRPA1.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-10-21 03:00:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/1253491"], "description"=>"<p><b>A:</b>Box plot diagram depicting membrane potential changes in TG neurons challenged with PEE (n = 31), sandalore (n = 31), sandranol (n = 31), javanol (n = 31), vanillin (n = 68), HTPA (n = 96), helional (n = 129) and geraniol (n = 108). Significant depolarization was evoked by vanillin, HTPA, helional, and geraniol. Amplitudes can be derived from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077998#pone-0077998-t001\" target=\"_blank\">table 1</a>. <b>B:</b> Exemplary whole-cell CC recordings of TG neurons upon stimulation with PEE, sandalore, sandranol, javanol, vanillin, HTPA, helional, and geraniol (1 mM each). RMP: PEE: −58 mV; sandalore: −62 mV; sandranol: −62 mV; javanol: −55 mV; vanillin: −60 mV; HTPA: −61 mV; helional: −58 mV; geraniol: −55 mV. <b>C,D:</b> Box plot diagrams depicting densities (pA/pF) of activated (C) and inhibited (D) currents during whole-cell VC recordings from TG neurons upon stimulation with helional (n = 33) and geraniol (n = 32). Outward currents recorded at +100 mV are depicted by upward facing bars, inward currents recorded at −100 mV are depicted by downward facing bars. Detailed values can be derived from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077998#pone-0077998-t002\" target=\"_blank\">table 2</a>. <b>E,F:</b> Whole-cell VC recordings from TG neurons challenged with vanillin, sandalore, helional, geraniol, cap, and ATP depicting odorant-induced activation (E) and inhibition (F) of currents. Current amplitudes at +100 (<i>black</i>) and −100 mV (<i>gray</i>) are plotted vs. time (<i>above</i>). Stimulus application is indicated by the gray bars. Colored arrows and roman numerals assign to individual voltage ramps depicted in the corresponding IV-plots (<i>below</i>).</p>", "links"=>[], "tags"=>["odorants", "activate", "cultured", "tg"], "article_id"=>829050, "categories"=>["Biological Sciences"], "users"=>["Matthias Lübbert", "Jessica Kyereme", "Nicole Schöbel", "Leopoldo Beltrán", "Christian Horst Wetzel", "Hanns Hatt"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0077998.g001", "stats"=>{"downloads"=>0, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Different_odorants_activate_cultured_TG_neurons_/829050", "title"=>"Different odorants activate cultured TG neurons.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-21 03:00:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/1253504"], "description"=>"<p>Reversal potentials (E<sub>rev</sub>) of helional-, geraniol-, and capsaicin-evoked Cs<sup>+</sup>-based currents.</p>", "links"=>[], "tags"=>["potentials", "capsaicin-evoked"], "article_id"=>829063, "categories"=>["Biological Sciences"], "users"=>["Matthias Lübbert", "Jessica Kyereme", "Nicole Schöbel", "Leopoldo Beltrán", "Christian Horst Wetzel", "Hanns Hatt"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0077998.t003", "stats"=>{"downloads"=>3, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Reversal_potentials_E_rev_of_helional_geraniol_and_capsaicin_evoked_Cs_based_currents_/829063", "title"=>"Reversal potentials (E<sub>rev</sub>) of helional-, geraniol-, and capsaicin-evoked Cs<sup>+</sup>-based currents.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-10-21 03:00:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/1253505"], "description"=>"<p>Amplitudes of TG neuron depolarizations triggered by administration of odorants and odorants plus the TRP channel antagonists CPZ, BCTC, and AITC.</p>", "links"=>[], "tags"=>["tg", "neuron", "depolarizations", "triggered", "odorants", "trp", "antagonists"], "article_id"=>829064, "categories"=>["Biological Sciences"], "users"=>["Matthias Lübbert", "Jessica Kyereme", "Nicole Schöbel", "Leopoldo Beltrán", "Christian Horst Wetzel", "Hanns Hatt"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0077998.t004", "stats"=>{"downloads"=>7, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Amplitudes_of_TG_neuron_depolarizations_triggered_by_administration_of_odorants_and_odorants_plus_the_TRP_channel_antagonists_CPZ_BCTC_and_AITC_/829064", "title"=>"Amplitudes of TG neuron depolarizations triggered by administration of odorants and odorants plus the TRP channel antagonists CPZ, BCTC, and AITC.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-10-21 03:00:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/1253503"], "description"=>"<p>In: inwardly directed currents at −100 mV; out: outwardly directed currents at +100 mV.</p>", "links"=>[], "tags"=>["currents", "densities", "tg", "neuron"], "article_id"=>829062, "categories"=>["Biological Sciences"], "users"=>["Matthias Lübbert", "Jessica Kyereme", "Nicole Schöbel", "Leopoldo Beltrán", "Christian Horst Wetzel", "Hanns Hatt"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0077998.t002", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Activated_currents_expressed_as_current_densities_in_distinct_TG_neuron_populations_/829062", "title"=>"Activated currents expressed as current densities in distinct TG neuron populations.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-10-21 03:00:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/1253499"], "description"=>"<p><b>A:</b> Box plot diagram depicting normalized rTRPA1-mediated currents evoked by vanillin (n = 11), HTPA (n = 11), helional (n = 11), and geraniol (n = 8) during VC recordings. Outward currents recorded at +100 mV are depicted as upward facing bars, inward currents recorded at −100 mV are depicted as downward facing bars. Detailed values can be derived from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077998#pone-0077998-t005\" target=\"_blank\">table 5</a>. <b>B:</b> Box plot diagram depicting normalized rTRPA1-mediated currents triggered by vanillin (n = 8), HTPA (n = 7), and helional (n = 9) that are inhibited by HC (exemplary recordings are depicted in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077998#pone.0077998.s003\" target=\"_blank\">fig. S3B</a>). Outward currents recorded at +100 mV are depicted as upward facing bars, inward currents recorded at −100 mV are depicted as downward facing bars. Detailed values can be derived from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077998#pone-0077998-t006\" target=\"_blank\">table 6</a>. <b>C-F:</b> Whole-cell VC recordings of CHO cells functionally expressing rTRPA1, challenged with vanillin (C), HTPA (D), helional (E), geraniol (F) and AITC. Amplitudes of currents at +100 (<i>black</i>) and −100 mV (<i>gray</i>) are plotted vs. time. Colored arrows and roman numerals assign to individual voltage ramps shown as current traces in the IV-plots depicted right sided, respectively. Stimulus applications are indicated by gray bars.</p>", "links"=>[], "tags"=>["activate", "functionally"], "article_id"=>829058, "categories"=>["Biological Sciences"], "users"=>["Matthias Lübbert", "Jessica Kyereme", "Nicole Schöbel", "Leopoldo Beltrán", "Christian Horst Wetzel", "Hanns Hatt"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0077998.g007", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Odorants_activate_functionally_expressed_rTRPA1_/829058", "title"=>"Odorants activate functionally expressed rTRPA1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-21 03:00:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/1253494"], "description"=>"<p>Exemplary CC recordings from cultured TG neurons upon stimulation with vanillin, HTPA, helional, and geraniol alone and with CPZ. Membrane potential changes and RMP of recorded neurons upon stimulation with: vanillin<sub>cap-sensitive</sub>: RMP: −45 mV; vanillin: Δ10 mV, vanillin+CPZ: Δ7 mV, cap: Δ20 mV, men: Δ0 mV; vanillin<sub>men-sensitive</sub>: RMP: −62 mV; vanillin: Δ0 mV, vanillin+CPZ: Δ0 mV, cap: Δ0 mV, men: Δ29,5 mV; HTPA<sub>cap-sensitive</sub>; RMP: −67 mV; HTPA: Δ14.6 mV, HTPA+CPZ: Δ10.3 mV, cap: Δ27.5 mV, Δ2.6 mV; HTPA<sub>men-sensitive</sub>; RMP: −61 mV; HTPA: Δ6.3 mV, Δ3.7 mV, cap: 0 mV, men: Δ31.4 mV; helional<sub>cap-sensitive</sub>: RMP: −73 mV; helional: Δ29.1 mV, helional+CPZ: Δ22 mV, cap Δ67 mV, men: 0 mV; helional<sub>men-sensitive</sub>: RMP: −50 mV; helional Δ7.2 mV, helional+CPZ: Δ7.8 mV, cap: Δ0 mV; men: Δ25 mV; geraniol<sub>cap-sensitive</sub>: RMP: −67 mV; geraniol: Δ18 mV, geraniol+CPZ: Δ14 mV, cap: Δ53 mV, men Δ0 mV; geraniol<sub>men-sensitive</sub>: RMP: −60 mV; geraniol: Δ17.6 mV, geraniol+CPZ: Δ14.5 mV, cap: Δ2 mV; men: Δ23 mV. Capsaicin- and menthol-evoked action potentials were cut at −30 mV (vanillin), −20 mV (HTPA), 0 mV (helional), and at −10 mV (geraniol). Stimulus applications are indicated by highlighted (<i>gray</i>) regions.</p>", "links"=>[], "tags"=>["cultured", "tg", "neurons", "stimulated", "odorants"], "article_id"=>829053, "categories"=>["Biological Sciences"], "users"=>["Matthias Lübbert", "Jessica Kyereme", "Nicole Schöbel", "Leopoldo Beltrán", "Christian Horst Wetzel", "Hanns Hatt"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0077998.g003", "stats"=>{"downloads"=>1, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_CC_recording_of_cultured_TG_neurons_stimulated_with_odorants_in_the_presence_and_absence_of_CPZ_/829053", "title"=>"CC recording of cultured TG neurons stimulated with odorants in the presence and absence of CPZ.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-21 03:00:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/1253511", "https://ndownloader.figshare.com/files/1253512", "https://ndownloader.figshare.com/files/1253513", "https://ndownloader.figshare.com/files/1253515", "https://ndownloader.figshare.com/files/1253516", "https://ndownloader.figshare.com/files/1253517", "https://ndownloader.figshare.com/files/1253518", "https://ndownloader.figshare.com/files/1253519"], "description"=>"<div><p>Primary sensory afferents of the dorsal root and trigeminal ganglia constantly transmit sensory information depicting the individual’s physical and chemical environment to higher brain regions. Beyond the typical trigeminal stimuli (e.g. irritants), environmental stimuli comprise a plethora of volatile chemicals with olfactory components (odorants). In spite of a complete loss of their sense of smell, anosmic patients may retain the ability to roughly discriminate between different volatile compounds. While the detailed mechanisms remain elusive, sensory structures belonging to the trigeminal system seem to be responsible for this phenomenon. In order to gain a better understanding of the mechanisms underlying the activation of the trigeminal system by volatile chemicals, we investigated odorant-induced membrane potential changes in cultured rat trigeminal neurons induced by the odorants vanillin, heliotropyl acetone, helional, and geraniol. We observed the dose-dependent depolarization of trigeminal neurons upon application of these substances occurring in a stimulus-specific manner and could show that distinct neuronal populations respond to different odorants. Using specific antagonists, we found evidence that TRPA1, TRPM8, and/or TRPV1 contribute to the activation. In order to further test this hypothesis, we used recombinantly expressed rat and human variants of these channels to investigate whether they are indeed activated by the odorants tested. We additionally found that the odorants dose-dependently inhibit two-pore potassium channels TASK1 and TASK3 heterologously expressed In <i>Xenopus laevis</i> oocytes. We suggest that the capability of various odorants to activate different TRP channels and to inhibit potassium channels causes neuronal depolarization and activation of distinct subpopulations of trigeminal sensory neurons, forming the basis for a specific representation of volatile chemicals in the trigeminal ganglia.</p></div>", "links"=>[], "tags"=>["receptor", "channels", "encode", "volatile", "chemicals", "sensed", "trigeminal", "ganglion"], "article_id"=>829070, "categories"=>["Biological Sciences"], "users"=>["Matthias Lübbert", "Jessica Kyereme", "Nicole Schöbel", "Leopoldo Beltrán", "Christian Horst Wetzel", "Hanns Hatt"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0077998.s001", "https://dx.doi.org/10.1371/journal.pone.0077998.s002", "https://dx.doi.org/10.1371/journal.pone.0077998.s003", "https://dx.doi.org/10.1371/journal.pone.0077998.s004", "https://dx.doi.org/10.1371/journal.pone.0077998.s005", "https://dx.doi.org/10.1371/journal.pone.0077998.s006", "https://dx.doi.org/10.1371/journal.pone.0077998.s007", "https://dx.doi.org/10.1371/journal.pone.0077998.s008"], "stats"=>{"downloads"=>10, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transient_Receptor_Potential_Channels_Encode_Volatile_Chemicals_Sensed_by_Rat_Trigeminal_Ganglion_Neurons_/829070", "title"=>"Transient Receptor Potential Channels Encode Volatile Chemicals Sensed by Rat Trigeminal Ganglion Neurons", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-10-21 03:00:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/1253493"], "description"=>"<p><b>A:</b> Ca<sup>2+</sup> imaging recording of fura2/AM-loaded TG neurons. Cells were challenged with 1 mM HTPA, 50 µM AITC, 300 µM men, and 3.3 µM cap. Stimulus-application is indicated by gray bars. <b>B:</b> Bar charts depicting the overlap of responses to the odorants vanillin, HTPA, helional, or geraniol (1 mM each) and to the TRP-agonists cap (3.3 µM), men (300 µM), and AITC (50 µM).</p>", "links"=>[], "tags"=>["numbers", "tg", "neurons", "odorants", "trp"], "article_id"=>829052, "categories"=>["Biological Sciences"], "users"=>["Matthias Lübbert", "Jessica Kyereme", "Nicole Schöbel", "Leopoldo Beltrán", "Christian Horst Wetzel", "Hanns Hatt"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0077998.g002", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_High_numbers_of_TG_neurons_respond_to_odorants_and_TRP_agonists_/829052", "title"=>"High numbers of TG neurons respond to odorants and TRP agonists.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-21 03:00:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/1253508"], "description"=>"<p>Values of odorant-evoked currents evoked by administration of odorants alone and odorants plus the TRP channel antagonists CPZ, BCTC, and AITC.</p>", "links"=>[], "tags"=>["odorant-evoked", "currents", "evoked", "odorants", "trp", "antagonists"], "article_id"=>829066, "categories"=>["Biological Sciences"], "users"=>["Matthias Lübbert", "Jessica Kyereme", "Nicole Schöbel", "Leopoldo Beltrán", "Christian Horst Wetzel", "Hanns Hatt"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0077998.t006", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Values_of_odorant_evoked_currents_evoked_by_administration_of_odorants_alone_and_odorants_plus_the_TRP_channel_antagonists_CPZ_BCTC_and_AITC_/829066", "title"=>"Values of odorant-evoked currents evoked by administration of odorants alone and odorants plus the TRP channel antagonists CPZ, BCTC, and AITC.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-10-21 03:00:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/1253502"], "description"=>"<p>Averaged amplitudes of vanillin-, HTPA-, helional-, and geraniol-evoked depolariza-tions of cultured TG neurons.</p>", "links"=>[], "tags"=>["amplitudes", "geraniol-evoked", "depolariza-tions", "cultured", "tg"], "article_id"=>829061, "categories"=>["Biological Sciences"], "users"=>["Matthias Lübbert", "Jessica Kyereme", "Nicole Schöbel", "Leopoldo Beltrán", "Christian Horst Wetzel", "Hanns Hatt"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0077998.t001", "stats"=>{"downloads"=>3, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Averaged_amplitudes_of_vanillin_HTPA_helional_and_geraniol_evoked_depolariza_tions_of_cultured_TG_neurons_/829061", "title"=>"Averaged amplitudes of vanillin-, HTPA-, helional-, and geraniol-evoked depolariza-tions of cultured TG neurons.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-10-21 03:00:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/1253501"], "description"=>"<p><b>A:</b> Exemplary VC recording of rTASK1-mediated voltage-driven currents upon administration of helional, vanillin, geraniol, and HTPA. <b>B:</b> Dose-response relations for vanillin, HTPA, helional, and geraniol, obtained from rTASK1 expressing <i>Xenopus laevis</i> oocytes during VC recordings. Compounds were administered in concentrations of 0.03, 0.1, 0.3, 1, 3, and 10 mM in ascending order. Data points present mean values and error bars present standard errors. <b>C:</b> Exemplary VC recording of TASK3-mediated voltage-driven currents during administration HTPA in arising concentrations. <b>D:</b> Bar diagram depicting the influence of varying concentrations of vanillin, HTPA, helional, and geraniol on voltage-driven currents via rTASK3 during VC recordings from <i>Xenopus laevis</i> oocytes. Bars present mean values and error bars present standard errors.</p>", "links"=>[], "tags"=>["dependently", "inhibit", "task1"], "article_id"=>829060, "categories"=>["Biological Sciences"], "users"=>["Matthias Lübbert", "Jessica Kyereme", "Nicole Schöbel", "Leopoldo Beltrán", "Christian Horst Wetzel", "Hanns Hatt"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0077998.g008", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Odorants_dose_dependently_inhibit_TASK1_and_TASK3_/829060", "title"=>"Odorants dose dependently inhibit TASK1 and TASK3.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-21 03:00:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/1253498"], "description"=>"<p><b>A:</b> Box plot diagram depicting normalized rTRPM8-mediated currents evoked by vanillin (n = 14), HTPA (n = 17), helional (n = 17), and geraniol (n = 17) during VC recordings. Outward currents recorded at +100 mV are depicted as upward facing bars, inward currents recorded at −100 mV are depicted as downward facing bars. Detailed values can be derived from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077998#pone-0077998-t005\" target=\"_blank\">table 5</a>. <b>B:</b> Whole-cell VC recordings of CHO cells functionally expressing rTRPM8, challenged with vanillin, HTPA, helional, geraniol and men. Amplitudes of currents at +100 (<i>black</i>) and -100 mV (<i>gray</i>) are plotted vs. time. Colored arrows and roman numerals assign to individual voltage ramps shown as current traces in the IV-plots depicted in <b>C</b>. Stimulus applications are indicated by gray bars. <b>D:</b> Box plot diagram depicting normalized rTRPM8-mediated currents triggered by HTPA (n =  12), helional (n = 14), and geraniol (n = 14) that are inhibited by CPZ (exemplary recordings are depicted in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077998#pone.0077998.s004\" target=\"_blank\">fig. S4A</a>). Outward currents recorded at +100 mV are depicted as upward facing bars, inward currents recorded at −100 mV are depicted as downward facing bars. Detailed values can be derived from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077998#pone-0077998-t006\" target=\"_blank\">table 6</a>. <b>E</b>: Box plot diagram depicting normalized rTRPM8-mediated currents triggered by HTPA helional, and geraniol that are inhibited by BCTC (exemplary recordings are depicted in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077998#pone.0077998.s004\" target=\"_blank\">fig. S4B</a>). Outward currents recorded at +100 mV are depicted as upward facing bars, inward currents recorded at −100 mV are depicted as downward facing bars. Detailed values can be derived from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077998#pone-0077998-t006\" target=\"_blank\">table 6</a>.</p>", "links"=>[], "tags"=>["activate", "functionally"], "article_id"=>829057, "categories"=>["Biological Sciences"], "users"=>["Matthias Lübbert", "Jessica Kyereme", "Nicole Schöbel", "Leopoldo Beltrán", "Christian Horst Wetzel", "Hanns Hatt"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0077998.g006", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Odorants_activate_functionally_expressed_rTRPM8_/829057", "title"=>"Odorants activate functionally expressed rTRPM8.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-21 03:00:39"}
  • {"files"=>["https://ndownloader.figshare.com/files/1253496"], "description"=>"<p><b>A:</b> Box plot diagram depicting normalized rTRPV1-mediated currents evoked by vanillin (n = 10), HTPA (n = 17), helional (n = 17), and geraniol (n = 17) during VC recordings. Outward currents recorded at +100 mV are depicted as upward facing bars, inward currents recorded at −100 mV are depicted as downward facing bars. Detailed values can be derived from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077998#pone-0077998-t005\" target=\"_blank\">table 5</a>. <b>B:</b> Whole-cell VC recordings of CHO cells functionally expressing rTRPV1, challenged with vanillin, HTPA, helional, geraniol and cap. Amplitudes of currents at +100 (<i>black</i>) and −100 mV (<i>gray</i>) are plotted vs. time. Colored arrows and roman numerals assign to individual voltage ramps shown as current traces in the IV-plots depicted in <b>C</b>. Stimulus applications are indicated by gray bars. <b>D:</b> Box plot diagram depicting normalized rTRPV1-mediated currents triggered by vanillin (n = 11), HTPA (n = 9), helional (n = 10), and geraniol (n = 10) that are inhibited by CPZ (exemplary recordings are depicted in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077998#pone.0077998.s003\" target=\"_blank\">fig. S3A</a>). Outward currents recorded at +100 mV are depicted as upward facing bars, inward currents recorded at −100 mV are depicted as downward facing bars. Detailed values can be derived from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0077998#pone-0077998-t006\" target=\"_blank\">table 6</a>.</p>", "links"=>[], "tags"=>["activate", "functionally"], "article_id"=>829055, "categories"=>["Biological Sciences"], "users"=>["Matthias Lübbert", "Jessica Kyereme", "Nicole Schöbel", "Leopoldo Beltrán", "Christian Horst Wetzel", "Hanns Hatt"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0077998.g005", "stats"=>{"downloads"=>0, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Odorants_activate_functionally_expressed_rTRPV1_/829055", "title"=>"Odorants activate functionally expressed rTRPV1.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-10-21 03:00:39"}

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