Distinct Expression Patterns of ICK/MAK/MOK Protein Kinases in the Intestine Implicate Functional Diversity
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{"title"=>"Distinct expression patterns of ICK/MAK/MOK protein kinases in the intestine implicate functional diversity", "type"=>"journal", "authors"=>[{"first_name"=>"Tufeng", "last_name"=>"Chen", "scopus_author_id"=>"7405543453"}, {"first_name"=>"Di", "last_name"=>"Wu", "scopus_author_id"=>"55554185000"}, {"first_name"=>"Christopher A.", "last_name"=>"Moskaluk", "scopus_author_id"=>"7003905170"}, {"first_name"=>"Zheng", "last_name"=>"Fu", "scopus_author_id"=>"55457255400"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "pmid"=>"24244486", "scopus"=>"2-s2.0-84892405056", "doi"=>"10.1371/journal.pone.0079359", "pui"=>"372116765", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "sgr"=>"84892405056"}, "id"=>"efa9a04f-d604-3673-8bae-717d40c7e776", "abstract"=>"ICK/MRK (intestinal cell kinase/MAK-related kinase), MAK (male germ cell-associated kinase), and MOK (MAPK/MAK/MRK-overlapping kinase) are closely related serine/threonine protein kinases in the protein kinome. The biological functions and regulatory mechanisms of the ICK/MAK/MOK family are still largely elusive. Despite significant similarities in their catalytic domains, they diverge markedly in the sequence and structural organization of their C-terminal non-catalytic domains, raising the question as to whether they have distinct, overlapping, or redundant biological functions. In order to gain insights into their biological activities and lay a fundamental groundwork for functional studies, we investigated the spatio-temporal distribution patterns and the expression dynamics of ICK/MAK/MOK protein kinases in the intestine. We found that ICK/MAK/MOK proteins display divergent expression patterns along the duodenum-to-colon axis and during postnatal murine development. Furthermore, they are differentially partitioned between intestinal epithelium and mesenchyme. A significant increase in the protein level of ICK, but not MAK, was induced in human primary colon cancer specimens. ICK protein level was up-regulated whereas MOK protein level was down-regulated in mouse intestinal adenomas as compared with their adjacent normal intestinal mucosa. These data suggest distinct roles for ICK/MAK/MOK protein kinases in the regulation of intestinal neoplasia. Taken together, our findings demonstrate that the expressions of ICK/MAK/MOK proteins in the intestinal tract can be differentially and dynamically regulated, implicating a significant functional diversity within this group of protein kinases.", "link"=>"http://www.mendeley.com/research/distinct-expression-patterns-ickmakmok-protein-kinases-intestine-implicate-functional-diversity", "reader_count"=>14, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Researcher"=>4, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>2, "Student > Postgraduate"=>1, "Student > Master"=>2, "Student > Bachelor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Researcher"=>4, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>2, "Student > Postgraduate"=>1, "Student > Master"=>2, "Student > Bachelor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>7, "Medicine and Dentistry"=>1, "Neuroscience"=>1, "Computer Science"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Neuroscience"=>{"Neuroscience"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>7}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"United Kingdom"=>1}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1271147"], "description"=>"<p>(A) Equal amount of total proteins (50 µg) extracted from various frozen tissues of young adult C57BL/6J mice (8 week-old) were Western blotted against antibodies recognizing ICK, MAK, and MOK respectively. Western blot signal of ERK2, closely related to ICK/MAK/MOK, was also shown as a control to indicate a universal expression pattern in various young adult mouse tissues. (B) Equal amount of total proteins (50 µg) extracted from frozen tissues (lung, liver, kidney and intestine) of mouse embryos of E13.5 and E16.5 were Western blotted against antibodies recognizing ICK, MAK, and MOK respectively. Western blot signals of ERK1/2 were shown as controls to indicate a universal expression pattern in various mouse embryonic tissues.</p>", "links"=>[], "tags"=>["proteins", "embryonic"], "article_id"=>843710, "categories"=>["Biological Sciences"], "users"=>["Tufeng Chen", "Di Wu", "Christopher A. Moskaluk", "Zheng Fu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0079359.g002", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_of_ICK_MAK_MOK_proteins_in_young_adult_and_embryonic_mouse_tissues_/843710", "title"=>"Expression of ICK/MAK/MOK proteins in young adult and embryonic mouse tissues.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-07 02:50:11"}
  • {"files"=>["https://ndownloader.figshare.com/files/1271156", "https://ndownloader.figshare.com/files/1271157", "https://ndownloader.figshare.com/files/1271158"], "description"=>"<div><p>ICK/MRK (intestinal cell kinase/MAK-related kinase), MAK (male germ cell-associated kinase), and MOK (MAPK/MAK/MRK-overlapping kinase) are closely related serine/threonine protein kinases in the protein kinome. The biological functions and regulatory mechanisms of the ICK/MAK/MOK family are still largely elusive. Despite significant similarities in their catalytic domains, they diverge markedly in the sequence and structural organization of their C-terminal non-catalytic domains, raising the question as to whether they have distinct, overlapping, or redundant biological functions. In order to gain insights into their biological activities and lay a fundamental groundwork for functional studies, we investigated the spatio-temporal distribution patterns and the expression dynamics of ICK/MAK/MOK protein kinases in the intestine. We found that ICK/MAK/MOK proteins display divergent expression patterns along the duodenum-to-colon axis and during postnatal murine development. Furthermore, they are differentially partitioned between intestinal epithelium and mesenchyme. A significant increase in the protein level of ICK, but not MAK, was induced in human primary colon cancer specimens. ICK protein level was up-regulated whereas MOK protein level was down-regulated in mouse intestinal adenomas as compared with their adjacent normal intestinal mucosa. These data suggest distinct roles for ICK/MAK/MOK protein kinases in the regulation of intestinal neoplasia. Taken together, our findings demonstrate that the expressions of ICK/MAK/MOK proteins in the intestinal tract can be differentially and dynamically regulated, implicating a significant functional diversity within this group of protein kinases.</p></div>", "links"=>[], "tags"=>["patterns", "kinases", "intestine", "implicate"], "article_id"=>843719, "categories"=>["Biological Sciences"], "users"=>["Tufeng Chen", "Di Wu", "Christopher A. Moskaluk", "Zheng Fu"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0079359.s001", "https://dx.doi.org/10.1371/journal.pone.0079359.s002", "https://dx.doi.org/10.1371/journal.pone.0079359.s003"], "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Distinct_Expression_Patterns_of_ICK_MAK_MOK_Protein_Kinases_in_the_Intestine_Implicate_Functional_Diversity_/843719", "title"=>"Distinct Expression Patterns of ICK/MAK/MOK Protein Kinases in the Intestine Implicate Functional Diversity", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-11-07 02:50:11"}
  • {"files"=>["https://ndownloader.figshare.com/files/1271152"], "description"=>"<p>Intestinal epithelial cells and mesenchymal cells were isolated and enriched from small intestine (A) and colon (B) tissues of young adult C57BL/6J mice. ICK/MAK/MOK protein signals in these two distinct cell populations were analyzed on Western blot. The E-Cadherin signal was used as a marker for epithelial cells and the VIM (Vimentin) and α-SMA (α-Smooth Muscle Actin) signals were used as markers for mesenchymal cells. The ERK1/2 signals were shown to indicate an equal distribution pattern between the epithelial and mesenchymal cell populations.</p>", "links"=>[], "tags"=>["partition", "proteins", "intestinal", "epithelial", "mesenchymal"], "article_id"=>843715, "categories"=>["Biological Sciences"], "users"=>["Tufeng Chen", "Di Wu", "Christopher A. Moskaluk", "Zheng Fu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0079359.g005", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Differential_partition_of_ICK_MAK_MOK_proteins_between_intestinal_epithelial_and_mesenchymal_cells_/843715", "title"=>"Differential partition of ICK/MAK/MOK proteins between intestinal epithelial and mesenchymal cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-07 02:50:11"}
  • {"files"=>["https://ndownloader.figshare.com/files/1271146"], "description"=>"<p>Although ICK, MAK and MOK protein kinases are very similar in their N-terminal catalytic domains, they differ markedly in the structural organization and features in their C-terminal non-catalytic domains. Note that the antigen peptides for ICK, MAK, and MOK proteins are all located at the C-terminal domain by design so as to ensure the antibody specificity.</p>", "links"=>[], "tags"=>["domains", "murine", "mak", "mok"], "article_id"=>843709, "categories"=>["Biological Sciences"], "users"=>["Tufeng Chen", "Di Wu", "Christopher A. Moskaluk", "Zheng Fu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0079359.g001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Schematic_illustration_of_the_structural_domains_and_features_of_murine_ICK_MAK_and_MOK_protein_kinases_/843709", "title"=>"Schematic illustration of the structural domains and features of murine ICK, MAK and MOK protein kinases.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-07 02:50:11"}
  • {"files"=>["https://ndownloader.figshare.com/files/1271154"], "description"=>"<p>(A) Tissue specimens macro-dissected from intestinal adenomatous polyps and their adjacent normal mucosa were obtained from C57BL/6J-Apc<sup>min</sup>/J mice (The Jackson Lab). Equal amount of total proteins (30 µg) from tissue extracts were Western blotted against ICK and MOK antibodies respectively. PCNA signal was shown as a proliferation marker to indicate malignant proliferation in the intestinal adenomas. Western signal of β-Actin was shown to indicate equal loading of total proteins. (B) Western blot signals of ICK and MOK were quantified by densitometry and normalized against the β-Actin signal. Shown here were the protein levels of ICK and MAK in intestinal adenomas relative to their adjacent normal tissues.</p>", "links"=>[], "tags"=>["mok", "proteins", "displayed", "patterns", "intestinal"], "article_id"=>843717, "categories"=>["Biological Sciences"], "users"=>["Tufeng Chen", "Di Wu", "Christopher A. Moskaluk", "Zheng Fu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0079359.g007", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ICK_and_MOK_proteins_displayed_opposite_expression_patterns_in_mouse_intestinal_adenomas_/843717", "title"=>"ICK and MOK proteins displayed opposite expression patterns in mouse intestinal adenomas.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-07 02:50:11"}
  • {"files"=>["https://ndownloader.figshare.com/files/1271153"], "description"=>"<p>(A) Tissue extracts from nine pairs of human specimens macro-dissected from primary colon tumor (T) and its surrounding normal mucosa (N) were prepared. Equal amount of total proteins (30 µg) from tissue extracts were loaded for Western blot. ERK2 signals indicate equal loading of total proteins. PCNA was used as a proliferation marker. (B) Western blot signals of ICK and MAK were quantified by densitometry and normalized against ERK2 signals. ICK and MAK protein levels in tumors relative to their adjacent normal tissues were shown as mean ± SE, n = 9, P<0.05, Paired <i>t</i>-Test.</p>", "links"=>[], "tags"=>["mak", "proteins", "exhibited", "patterns", "colorectal"], "article_id"=>843716, "categories"=>["Biological Sciences"], "users"=>["Tufeng Chen", "Di Wu", "Christopher A. Moskaluk", "Zheng Fu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0079359.g006", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ICK_and_MAK_proteins_exhibited_distinct_expression_patterns_in_human_colorectal_tumors_/843716", "title"=>"ICK and MAK proteins exhibited distinct expression patterns in human colorectal tumors.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-07 02:50:11"}
  • {"files"=>["https://ndownloader.figshare.com/files/1271150"], "description"=>"<p>The small intestine or colon was rapidly dissected from four littermates of postnatal day (P) p1, p4, p7, p14, and p21. Tissue extracts were prepared from frozen intestinal tissues. Equal amount of total proteins (50 µg) were loaded on Western blot against ICK, MAK, and MOK antibodies respectively. The β-Actin signal was shown on Western blot to indicate equal loading of total proteins.</p>", "links"=>[], "tags"=>["proteins", "intestine", "postnatal"], "article_id"=>843713, "categories"=>["Biological Sciences"], "users"=>["Tufeng Chen", "Di Wu", "Christopher A. Moskaluk", "Zheng Fu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0079359.g004", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_of_ICK_MAK_MOK_proteins_in_the_mouse_intestine_during_postnatal_development_/843713", "title"=>"Expression of ICK/MAK/MOK proteins in the mouse intestine during postnatal development.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-07 02:50:11"}
  • {"files"=>["https://ndownloader.figshare.com/files/1271148"], "description"=>"<p>The intestinal tube was rapidly dissected from four littermates of postnatal day 21 and segmented into duodenum (D), proximal jejunum (PJ), distal jejunum (PJ), ileum (I), and colon (C) respectively. Tissue extracts were prepared from frozen intestinal tissues. Equal amount of total proteins (50 µg) were loaded on Western blot against ICK, MAK, and MOK antibodies respectively. ERK signal was shown to demonstrate an equal distribution pattern along the duodenum-to-colon axis, whereas AKT signal was shown to demonstrate a colon-enriched distribution pattern under the normal steady-state condition.</p>", "links"=>[], "tags"=>["proteins", "duodenum-to-colon", "axis"], "article_id"=>843711, "categories"=>["Biological Sciences"], "users"=>["Tufeng Chen", "Di Wu", "Christopher A. Moskaluk", "Zheng Fu"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0079359.g003", "stats"=>{"downloads"=>0, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_of_ICK_MAK_MOK_proteins_along_the_duodenum_to_colon_axis_in_the_intestine_/843711", "title"=>"Expression of ICK/MAK/MOK proteins along the duodenum-to-colon axis in the intestine.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-07 02:50:11"}

PMC Usage Stats | Further Information

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  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}

Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[269, 466, 588, 697, 800, 896, 988, 1076, 1165, 1254, 1340, 1417]}, {"subject_area"=>"/Biology and life sciences/Anatomy", "average_usage"=>[248, 440, 562, 666, 765, 856, 944, 1028, 1112, 1198, 1282, 1362, 1430]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[272, 466, 589, 702, 806, 903, 995, 1086, 1176, 1258, 1347, 1422, 1493]}, {"subject_area"=>"/Medicine and health sciences/Anatomy", "average_usage"=>[248, 441, 564, 668, 769, 859, 948, 1034, 1117, 1202, 1290, 1368, 1437]}, {"subject_area"=>"/Medicine and health sciences/Oncology", "average_usage"=>[249, 468, 599, 718, 820, 920, 1008, 1093, 1181, 1281, 1357, 1444, 1517]}, {"subject_area"=>"/People and places/Population groupings", "average_usage"=>[265, 456, 578, 682, 782, 881, 965, 1053, 1133, 1223, 1310, 1391, 1457]}]}
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