Immunolocalization of an Amino-Terminal Fragment of Apolipoprotein E in the Pick's Disease Brain
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{"title"=>"Immunolocalization of an amino-terminal fragment of apolipoprotein E in the Pick's disease brain", "type"=>"journal", "authors"=>[{"first_name"=>"Troy T.", "last_name"=>"Rohn", "scopus_author_id"=>"7004490741"}, {"first_name"=>"Ryan J.", "last_name"=>"Day", "scopus_author_id"=>"55991441300"}, {"first_name"=>"Lindsey W.", "last_name"=>"Catlin", "scopus_author_id"=>"41560986600"}, {"first_name"=>"Raquel J.", "last_name"=>"Brown", "scopus_author_id"=>"7406360915"}, {"first_name"=>"Alexander J.", "last_name"=>"Rajic", "scopus_author_id"=>"55990288500"}, {"first_name"=>"Wayne W.", "last_name"=>"Poon", "scopus_author_id"=>"7103025394"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84891786369", "pui"=>"372065608", "doi"=>"10.1371/journal.pone.0080180", "pmid"=>"24312462", "scopus"=>"2-s2.0-84891786369", "issn"=>"19326203", "isbn"=>"1932-6203"}, "id"=>"2a0e98bd-ad81-3a0a-8247-114f1d7ec8fd", "abstract"=>"Although the risk factor for apolipoprotein E (apoE) polymorphism in Alzheimer's disease (AD) has been well described, the role that apoE plays in other neurodegenerative diseases, including Pick's disease, is not well established. To examine a possible role of apoE in Pick's disease, an immunohistochemical analysis was performed utilizing a novel site-directed antibody that is specific for an amino-terminal fragment of apoE. Application of this antibody, termed the amino-terminal apoE cleavage fragment (nApoECF) antibody, consistently labeled Pick bodies within area CA1 of the hippocampus in 4 of the 5 cases examined. Co-localization of the nApoECF antibody with PHF-1, a general marker for Pick bodies, as well as with an antibody to caspase-cleaved tau (TauC3) was evident within the hippocampus. While staining of the nApoECF antibody was robust in area CA1, little co-localization with PHF-1 in Pick bodies within the dentate gyrus was observed. A quantitative analysis indicated that approximately 86% of the Pick bodies identified in area CA1 labeled with the nApoECF antibody. The presence of truncated apoE within Pick bodies suggests a broader role of apoE beyond AD and raises the question as to whether this protein contributes to pathogenesis associated with Pick's disease.", "link"=>"http://www.mendeley.com/research/immunolocalization-aminoterminal-fragment-apolipoprotein-e-picks-disease-brain", "reader_count"=>7, "reader_count_by_academic_status"=>{"Librarian"=>1, "Researcher"=>2, "Student > Ph. D. Student"=>1, "Student > Master"=>1, "Student > Bachelor"=>1, "Student > Doctoral Student"=>1}, "reader_count_by_user_role"=>{"Librarian"=>1, "Researcher"=>2, "Student > Ph. D. Student"=>1, "Student > Master"=>1, "Student > Bachelor"=>1, "Student > Doctoral Student"=>1}, "reader_count_by_subject_area"=>{"Environmental Science"=>1, "Agricultural and Biological Sciences"=>2, "Medicine and Dentistry"=>1, "Neuroscience"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Social Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Neuroscience"=>{"Neuroscience"=>1}, "Social Sciences"=>{"Social Sciences"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>2}, "Environmental Science"=>{"Environmental Science"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"United States"=>1}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1301079"], "description"=>"<p>(<b>A and B</b>): Low (A) and high magnification (B) of representative labeling from a Pick's case (Case #4, <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080180#pone-0080180-t001\" target=\"_blank\">Table 1</a>) utilizing the nApoECF antibody illustrating staining in the CA1 region of the hippocampus within Pick bodies. (<b>C</b>): Representative staining in the dentate gyrus (Case #3, <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080180#pone-0080180-t001\" target=\"_blank\">Table 1</a>) showing weaker, sparser labeling with the nApoECF antibody. (<b>D</b>): Representative staining in case #5 (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080180#pone-0080180-t001\" target=\"_blank\">Table 1</a>), which had a primary neuropathological diagnosis of Pick's disease showing a complete lack of labeling of Pick bodies but instead labeling of apparent NFTs (arrow). (<b>E</b>): Representative staining of the nApoECF antibody in an age-matched control case revealing a complete absence of labeling. (<b>F</b>): Quantitative analysis of the number of Pick bodies that were nApoECF-positive for each Pick case examined with the nApoECF antibody. Data are the average of two independent counts ±S.D. For case demographics please see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080180#pone-0080180-t001\" target=\"_blank\"><b>Table 1</b></a>.</p>", "links"=>[], "tags"=>["amino-terminal", "apoe"], "article_id"=>866744, "categories"=>["Biological Sciences"], "users"=>["Troy T. Rohn", "Ryan J. Day", "Lindsey W. Catlin", "Raquel J. Brown", "Alexander J. Rajic", "Wayne W. Poon"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0080180.g001", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Localization_of_an_amino_terminal_fragment_of_apoE_in_Pick_s_disease_/866744", "title"=>"Localization of an amino-terminal fragment of apoE in Pick's disease.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-02 04:18:11"}
  • {"files"=>["https://ndownloader.figshare.com/files/1301082"], "description"=>"<p>(<b>A–C</b>): Representative immunofluorescence double-labeling in Case #4 (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080180#pone-0080180-t001\" target=\"_blank\">Table 1</a>) within the CA1 region of the hippocampus in Pick's disease utilizing the nApoECF antibody (green, Panel A), caspase-cleaved tau (red, Panel B) and with the overlap image shown in Panel C. All scale bars represent 10 µm. (<b>D</b>): Quantification of Pick bodies double-labeled by TauC3 (caspase-cleaved tau) and nApoECF in area CA1 of the hippocampus. Data show the number of Pick bodies labeled with nApoECF alone (blue bar), Tauc3 alone (green bar) or those Pick bodies that were labeled with both antibodies (red bar). Pick bodies were identified in a 20× field within area CA1 by immunofluorescence overlap microscopy (n = 3 fields for 4 different Pick cases) ±S.D., *p<0.05. Data indicated that roughly 75% of all labeled Pick bodies within area CA1 were co-localized with both antibodies. (<b>E–G</b>): Double-labeled confocal immunofluorescence images with the nApoECF antibody (green, E), PHF-1 (red, F), and the two images overlapped (G). (<b>H</b>): High magnification confocal overlapped image representing nApoECF (green channel, left), TauC3 (red channel, middle), and overlapped image (yellow/orange, right). Scale bars in Panel H represent 5 µm.</p>", "links"=>[], "tags"=>["napoecf", "antibody", "caspase-cleaved"], "article_id"=>866747, "categories"=>["Biological Sciences"], "users"=>["Troy T. Rohn", "Ryan J. Day", "Lindsey W. Catlin", "Raquel J. Brown", "Alexander J. Rajic", "Wayne W. Poon"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0080180.g003", "stats"=>{"downloads"=>1, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Co_localization_of_the_nApoECF_antibody_with_caspase_cleaved_tau_/866747", "title"=>"Co-localization of the nApoECF antibody with caspase-cleaved tau.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-02 04:18:11"}
  • {"files"=>["https://ndownloader.figshare.com/files/1301083"], "description"=>"<p>PMI, postmortem interval in hours; NPD, neuropathological diagnosis.</p>", "links"=>[], "tags"=>[], "article_id"=>866748, "categories"=>["Biological Sciences"], "users"=>["Troy T. Rohn", "Ryan J. Day", "Lindsey W. Catlin", "Raquel J. Brown", "Alexander J. Rajic", "Wayne W. Poon"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0080180.t001", "stats"=>{"downloads"=>0, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Case_Demographics_/866748", "title"=>"Case Demographics.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-12-02 04:18:11"}
  • {"files"=>["https://ndownloader.figshare.com/files/1301080"], "description"=>"<p>(<b>A–C</b>): Representative immunofluorescence double-labeling in Case #3 (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080180#pone-0080180-t001\" target=\"_blank\">Table 1</a>) utilizing the nApoECF antibody (green, Panel A) and PHF-1 (red, Panel B) revealed strong co-localization of the two antibodies within Pick bodies of area CA1 (Panel C). (<b>D–F</b>): In contrast to area CA1, strong immunofluorescence was detected using PHF-1 (red, Panel E), but only weak labeling was observed within granule cells of the dentate gyrus following application of the nApoECF antibody (green, Panel D). Panel F shows the overlap image with yellow indicating Pick bodies double-labeled with both antibodies. (<b>G</b>): Quantification of Pick bodies double-labeled by PHF-1 and nApoECF. Data show the number of Pick bodies labeled with nApoECF alone (blue bar), PHF-1 alone (green bar) or those Pick bodies that were labeled with both antibodies (red bar) in area CA1. Pick bodies were identified in a 20× field within area CA1 by immunofluorescence overlap microscopy (n = 3 fields for 4 different Pick cases) ±S.D., *p<0.05. Data indicated that roughly 86% of all identified Pick bodies within area CA1 were labeled with both antibodies. (<b>H–J</b>): Double-labeled confocal immunofluorescence images with the nApoECF antibody (green, H), PHF-1 (red, I), and the two images overlapped (J). All scale bars represent 10 µm, except for confocal images in Panel H–J, which represent 50 µm. (<b>K</b>): High magnification confocal overlapped image representing nApoECF (green channel, left), PHF-1 (red channel, middle), and overlapped image (yellow/orange, right); notice filamentous nature of staining within the two Pick bodies. Scale bars for Panel K represent 5 µm.</p>", "links"=>[], "tags"=>["napoecf", "antibody", "hippocampus", "co-localizes", "phf-1", "regionally"], "article_id"=>866745, "categories"=>["Biological Sciences"], "users"=>["Troy T. Rohn", "Ryan J. Day", "Lindsey W. Catlin", "Raquel J. Brown", "Alexander J. Rajic", "Wayne W. Poon"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0080180.g002", "stats"=>{"downloads"=>0, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Labeling_of_nApoECF_antibody_within_the_hippocampus_co_localizes_with_PHF_1_and_is_regionally_defined_/866745", "title"=>"Labeling of nApoECF antibody within the hippocampus co-localizes with PHF-1 and is regionally defined.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-02 04:18:11"}

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Relative Metric

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