EDA-Containing Fibronectin Increases Proliferation of Embryonic Stem Cells
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{"title"=>"EDA-containing fibronectin increases proliferation of embryonic stem cells", "type"=>"journal", "authors"=>[{"first_name"=>"Noelia", "last_name"=>"Losino", "scopus_author_id"=>"36053039500"}, {"first_name"=>"Ariel", "last_name"=>"Waisman", "scopus_author_id"=>"39763256600"}, {"first_name"=>"Claudia", "last_name"=>"Solari", "scopus_author_id"=>"39762728400"}, {"first_name"=>"Carlos", "last_name"=>"Luzzani", "scopus_author_id"=>"23012694700"}, {"first_name"=>"Darío Fernández", "last_name"=>"Espinosa", "scopus_author_id"=>"56028687100"}, {"first_name"=>"Alina", "last_name"=>"Sassone", "scopus_author_id"=>"51665745500"}, {"first_name"=>"Andrés F.", "last_name"=>"Muro", "scopus_author_id"=>"7006690111"}, {"first_name"=>"Santiago", "last_name"=>"Miriuka", "scopus_author_id"=>"6506986052"}, {"first_name"=>"Gustavo", "last_name"=>"Sevlever", "scopus_author_id"=>"6603518450"}, {"first_name"=>"Lino", "last_name"=>"Barañao", "scopus_author_id"=>"55665377900"}, {"first_name"=>"Alejandra", "last_name"=>"Guberman", "scopus_author_id"=>"7003979395"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"24244705", "sgr"=>"84893597067", "doi"=>"10.1371/journal.pone.0080681", "scopus"=>"2-s2.0-84893597067", "pui"=>"372325207", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "issn"=>"19326203"}, "id"=>"153de3db-418c-3ad5-8ad4-b1812882df7c", "abstract"=>"Embryonic stem cells (ESC) need a set of specific factors to be propagated. They can also grow in conditioned medium (CM) derived from a bovine granulosa cell line BGC (BGC-CM), a medium that not only preserves their main features but also increases ESC´s proliferation rate. The mitogenic properties of this medium were previously reported, ascribing this effect to an alternative spliced generated fibronectin isoform that contains the extra domain A (FN EDA(+)). Here, we investigated if the FN EDA(+) isoform increased proliferation of mouse and human ES cells. We analyzed cell proliferation using conditioned media produced by different mouse embryonic fibroblast (MEF) lines genetically engineered to express FN constitutively including or excluding the EDA domain (FN EDA(-)), and in media supplemented with recombinant peptides containing or not the EDA. We found that the presence of EDA in the medium increased mouse and human ESC's proliferation rate. Here we showed for the first time that this FN isoform enhances ESC's proliferation. These findings suggest a possible conserved behavior for regulation of ES cells proliferation by this FN isoform and could contribute to improve their culturing conditions both for research and cell therapy.", "link"=>"http://www.mendeley.com/research/edacontaining-fibronectin-increases-proliferation-embryonic-stem-cells", "reader_count"=>26, "reader_count_by_academic_status"=>{"Researcher"=>7, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>1, "Student > Master"=>4, "Student > Bachelor"=>2, "Professor"=>2}, "reader_count_by_user_role"=>{"Researcher"=>7, "Student > Doctoral Student"=>4, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>1, "Student > Master"=>4, "Student > Bachelor"=>2, "Professor"=>2}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>6, "Agricultural and Biological Sciences"=>17, "Medicine and Dentistry"=>2, "Neuroscience"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Neuroscience"=>{"Neuroscience"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>17}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}}, "reader_count_by_country"=>{"France"=>1}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1280358"], "description"=>"<p>WA09 hES cells were cultured for three days on Matrigel coated dishes in the indicated medium (untreated cells, C; EDA<sup>+/+</sup> MEF-CM, CM; EDA+ including peptide, PEP) and then <i>in </i><i>vitro</i> differentiated as described in Material and Methods Section for seven days. RNA was extracted and the expression of lineage specific gene markers was analyzed by RT-PCR. Alpha-fetoprotein (AFP), endoderm gene marker; Cardiac Troponin (CT), mesoderm gene marker; PAX6, ectoderm gene marker.</p>", "links"=>[], "tags"=>["esc", "differentiate", "germ"], "article_id"=>850389, "categories"=>["Biological Sciences"], "users"=>["Noelia Losino", "Ariel Waisman", "Claudia Solari", "Carlos Luzzani", "Darío Fernández Espinosa", "Alina Sassone", "Andrés F. Muro", "Santiago Miriuka", "Gustavo Sevlever", "Lino Barañao", "Alejandra Guberman"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0080681.g005", "stats"=>{"downloads"=>1, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_EDA_8211_treated_human_ESC_can_differentiate_to_the_three_germ_layers_/850389", "title"=>"EDA–treated human ESC can differentiate to the three germ layers.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-14 02:42:15"}
  • {"files"=>["https://ndownloader.figshare.com/files/1280357"], "description"=>"<p>WA09 human ES cells and Ainv15 mouse ES cells were cultured for three days on feeder free conditions in the indicated medium (untreated cells, C; EDA<sup>+/+</sup> MEF-CM, CM; EDA+ including peptide, PEP). RNA was extracted and the expression of Oct4, Sox2 and Nanog pluripotency gene markers was analyzed by RT-PCR. The expression of the housekeeping GAPDH gene was used as control.</p>", "links"=>[], "tags"=>["es", "cells", "pluripotency"], "article_id"=>850388, "categories"=>["Biological Sciences"], "users"=>["Noelia Losino", "Ariel Waisman", "Claudia Solari", "Carlos Luzzani", "Darío Fernández Espinosa", "Alina Sassone", "Andrés F. Muro", "Santiago Miriuka", "Gustavo Sevlever", "Lino Barañao", "Alejandra Guberman"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0080681.g004", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_EDA_8211_treated_ES_cells_express_pluripotency_markers_/850388", "title"=>"EDA –treated ES cells express pluripotency markers.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-14 02:42:15"}
  • {"files"=>["https://ndownloader.figshare.com/files/1280353"], "description"=>"<p>Ainv15 mES cells were cultured in the media conditioned by wild type MEF (wt), EDA<sup>+/+</sup> MEF (<sup>+/+</sup>) or EDA<sup>-/-</sup> MEF (<sup>-/-</sup>) for 2 days. The proliferation was evaluated by MTT assay. A representative experiment of at least three replicates is shown. Bars represent mean ± SD. Statistical analysis was done by an unpaired t-test. * indicate significant differences between treatments (<i>P</i> < 0.01).</p>", "links"=>[], "tags"=>["conditioned", "contains", "fn", "proliferation"], "article_id"=>850384, "categories"=>["Biological Sciences"], "users"=>["Noelia Losino", "Ariel Waisman", "Claudia Solari", "Carlos Luzzani", "Darío Fernández Espinosa", "Alina Sassone", "Andrés F. Muro", "Santiago Miriuka", "Gustavo Sevlever", "Lino Barañao", "Alejandra Guberman"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0080681.g001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_MEF_conditioned_medium_that_contains_FN_EDA_increases_the_proliferation_rate_of_mESC_/850384", "title"=>"MEF conditioned medium that contains FN EDA<sup>+</sup> increases the proliferation rate of mESC.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-14 02:42:15"}
  • {"files"=>["https://ndownloader.figshare.com/files/1280362", "https://ndownloader.figshare.com/files/1280363", "https://ndownloader.figshare.com/files/1280364", "https://ndownloader.figshare.com/files/1280365"], "description"=>"<div><p>Embryonic stem cells (ESC) need a set of specific factors to be propagated. They can also grow in conditioned medium (CM) derived from a bovine granulosa cell line BGC (BGC-CM), a medium that not only preserves their main features but also increases ESC´s proliferation rate. The mitogenic properties of this medium were previously reported, ascribing this effect to an alternative spliced generated fibronectin isoform that contains the extra domain A (FN EDA<sup>+</sup>). Here, we investigated if the FN EDA<sup>+</sup> isoform increased proliferation of mouse and human ES cells. We analyzed cell proliferation using conditioned media produced by different mouse embryonic fibroblast (MEF) lines genetically engineered to express FN constitutively including or excluding the EDA domain (FN EDA<sup>-</sup>), and in media supplemented with recombinant peptides containing or not the EDA. We found that the presence of EDA in the medium increased mouse and human ESC’s proliferation rate. Here we showed for the first time that this FN isoform enhances ESC’s proliferation. These findings suggest a possible conserved behavior for regulation of ES cells proliferation by this FN isoform and could contribute to improve their culturing conditions both for research and cell therapy.</p> </div>", "links"=>[], "tags"=>["fibronectin", "proliferation", "embryonic"], "article_id"=>850393, "categories"=>["Biological Sciences"], "users"=>["Noelia Losino", "Ariel Waisman", "Claudia Solari", "Carlos Luzzani", "Darío Fernández Espinosa", "Alina Sassone", "Andrés F. Muro", "Santiago Miriuka", "Gustavo Sevlever", "Lino Barañao", "Alejandra Guberman"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0080681.s001", "https://dx.doi.org/10.1371/journal.pone.0080681.s002", "https://dx.doi.org/10.1371/journal.pone.0080681.s003", "https://dx.doi.org/10.1371/journal.pone.0080681.s004"], "stats"=>{"downloads"=>9, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_EDA_Containing_Fibronectin_Increases_Proliferation_of_Embryonic_Stem_Cells_/850393", "title"=>"EDA-Containing Fibronectin Increases Proliferation of Embryonic Stem Cells", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-11-14 02:42:15"}
  • {"files"=>["https://ndownloader.figshare.com/files/1280360"], "description"=>"<p>Ainv15 mES cells were cultured for three days on gelatin coated dishes in the presence of EDA+ peptide. Then, 10<sup>6</sup> cells were injected subcutaneously into nude mice, as described in Material and Methods Section. Four-micrometer sections from teratoma tissue were stained with hematoxylin and eosin. (A–C) Representative histology of teratoma (A) Neuroepithelium (arrow, ectoderm) and mesenchymal tissue (star, mesoderm); (B) Neuroepithelium (arrow, ectoderm) and glial tissue (ectoderm); Glandular tissue (arrow, endoderm).</p>", "links"=>[], "tags"=>["esc", "differentiate", "germ"], "article_id"=>850391, "categories"=>["Biological Sciences"], "users"=>["Noelia Losino", "Ariel Waisman", "Claudia Solari", "Carlos Luzzani", "Darío Fernández Espinosa", "Alina Sassone", "Andrés F. Muro", "Santiago Miriuka", "Gustavo Sevlever", "Lino Barañao", "Alejandra Guberman"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0080681.g006", "stats"=>{"downloads"=>2, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_EDA_8211_treated_mouse_ESC_can_differentiate_to_the_three_germ_layers_/850391", "title"=>"EDA–treated mouse ESC can differentiate to the three germ layers.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-14 02:42:15"}
  • {"files"=>["https://ndownloader.figshare.com/files/1280356"], "description"=>"<p>WAO9 human ES cells were plated in standard proliferation medium. 24 hours later, when the cells were attached, medium was replaced by fresh medium containing the corresponding peptide preparation to a final dose of 10 µg of protein per ml of medium. (A) Representative brightfield pictures of the wound-healing assay. The scratch was made the same day that the peptide was added to the medium. Left panel, linear scratch; right panel, circular scratch. The references are the same than in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080681#pone-0080681-g002\" target=\"_blank\">Figure 2</a>. Bars correspond to 200 µm. (B) Quantification of the filled areas of a representative experiment of three replicates. Proliferation was calculated from the decrement in damaged area. The areas were quantified with the ImageJ software. The filled area in each condition was referred as the amount of filled area in the control, considered as 100%. The graph is representative of a scratch wound healing assay of three replicates.</p>", "links"=>[], "tags"=>["fn", "proliferation"], "article_id"=>850387, "categories"=>["Biological Sciences"], "users"=>["Noelia Losino", "Ariel Waisman", "Claudia Solari", "Carlos Luzzani", "Darío Fernández Espinosa", "Alina Sassone", "Andrés F. Muro", "Santiago Miriuka", "Gustavo Sevlever", "Lino Barañao", "Alejandra Guberman"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0080681.g003", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_FN_EDA_but_not_FN_EDA_increases_the_proliferation_of_human_ESC_/850387", "title"=>"FN EDA<sup>+</sup> but not FN EDA<sup>-</sup> increases the proliferation of human ESC.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-14 02:42:15"}
  • {"files"=>["https://ndownloader.figshare.com/files/1280355"], "description"=>"<p>(A) Ainv 15 mouse ES cells were cultured in the standard media supplemented by the corresponding peptide preparation to a final dose of 10 µg of protein per ml of medium for 72 hours. (EDA-containing recombinant peptide, EDA<sup>+</sup>; EDA-lacking recombinant peptide, EDA<sup>-</sup>). Representative brightfield pictures are shown. Bars correspond to 50 µm. (B) The proliferation of Ainv15 mES cells cultured in the media described in B for 48 or 72 hours, as indicated, was evaluated by crystal violet. A representative experiment of at least three replicates is shown. Bars represent mean ± SD. Statistical analysis was done by One way ANOVA with Bonferroni test for multiple comparisons. * indicate significant differences between treatments (<i>P</i> < 0.05); ** indicate significant differences between treatments (<i>P</i> < 0.005).</p>", "links"=>[], "tags"=>["peptide", "proliferation"], "article_id"=>850386, "categories"=>["Biological Sciences"], "users"=>["Noelia Losino", "Ariel Waisman", "Claudia Solari", "Carlos Luzzani", "Darío Fernández Espinosa", "Alina Sassone", "Andrés F. Muro", "Santiago Miriuka", "Gustavo Sevlever", "Lino Barañao", "Alejandra Guberman"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0080681.g002", "stats"=>{"downloads"=>1, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_EDA_but_not_EDA_peptide_increases_the_proliferation_rate_of_mESC_/850386", "title"=>"EDA<sup>+</sup> but not EDA<sup>-</sup> peptide increases the proliferation rate of mESC.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-11-14 02:42:15"}

PMC Usage Stats | Further Information

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Relative Metric

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