Rv0132c of Mycobacterium tuberculosis Encodes a Coenzyme F420-Dependent Hydroxymycolic Acid Dehydrogenase
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Rv0132c of Mycobacterium tuberculosis encodes a coenzyme F 420-dependent hydroxymycolic acid dehydrogenase", "type"=>"journal", "authors"=>[{"first_name"=>"Endang", "last_name"=>"Purwantini", "scopus_author_id"=>"6602297184"}, {"first_name"=>"Biswarup", "last_name"=>"Mukhopadhyay", "scopus_author_id"=>"7101857372"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"24349169", "sgr"=>"84892408300", "doi"=>"10.1371/journal.pone.0081985", "scopus"=>"2-s2.0-84892408300", "pui"=>"372116711", "isbn"=>"10.1371/journal.pone.0081985", "issn"=>"19326203"}, "id"=>"5c571cfe-885f-3448-aa33-a4f7bc4e0f8c", "abstract"=>"The ability of Mycobacterium tuberculosis to manipulate and evade human immune system is in part due to its extraordinarily complex cell wall. One of the key components of this cell wall is a family of lipids called mycolic acids. Oxygenation of mycolic acids generating methoxy- and ketomycolic acids enhances the pathogenic attributes of M. tuberculosis. Thus, the respective enzymes are of interest in the research on mycobacteria. The generation of methoxy- and ketomycolic acids proceeds through intermediary formation of hydroxymycolic acids. While the methyl transferase that generates methoxymycolic acids from hydroxymycolic acids is known, hydroxymycolic acids dehydrogenase that oxidizes hydroxymycolic acids to ketomycolic acids has been elusive. We found that hydroxymycolic acid dehydrogenase is encoded by the rv0132c gene and the enzyme utilizes F420, a deazaflavin coenzyme, as electron carrier, and accordingly we called it F420-dependent hydroxymycolic acid dehydrogenase. This is the first report on the involvement of F420 in the synthesis of a mycobacterial cell envelope. Also, F420-dependent hydroxymycolic acid dehydrogenase was inhibited by PA-824, and therefore, it is a previously unknown target for this new tuberculosis drug.", "link"=>"http://www.mendeley.com/research/rv0132c-mycobacterium-tuberculosis-encodes-coenzyme-f-420dependent-hydroxymycolic-acid-dehydrogenase", "reader_count"=>16, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Researcher"=>2, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>4, "Student > Postgraduate"=>2, "Student > Master"=>2, "Student > Bachelor"=>3}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Researcher"=>2, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>4, "Student > Postgraduate"=>2, "Student > Master"=>2, "Student > Bachelor"=>3}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>5, "Nursing and Health Professions"=>1, "Agricultural and Biological Sciences"=>6, "Chemistry"=>1, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Chemistry"=>{"Chemistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>6}, "Nursing and Health Professions"=>{"Nursing and Health Professions"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>5}, "Unspecified"=>{"Unspecified"=>1}}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84892408300"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84892408300?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84892408300&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84892408300&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84892408300", "dc:identifier"=>"SCOPUS_ID:84892408300", "eid"=>"2-s2.0-84892408300", "dc:title"=>"Rv0132c of Mycobacterium tuberculosis encodes a coenzyme F <inf>420</inf>-dependent hydroxymycolic acid dehydrogenase", "dc:creator"=>"Purwantini E.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"8", "prism:issueIdentifier"=>"12", "prism:pageRange"=>nil, "prism:coverDate"=>"2013-12-11", "prism:coverDisplayDate"=>"11 December 2013", "prism:doi"=>"10.1371/journal.pone.0081985", "citedby-count"=>"17", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Virginia Polytechnic Institute and State University", "affiliation-city"=>"Blacksburg", "affiliation-country"=>"United States"}, {"@_fa"=>"true", "affilname"=>"Biocomplexity Institute of Virginia Tech", "affiliation-city"=>"Blacksburg", "affiliation-country"=>"United States"}], "pubmed-id"=>"24349169", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e81985", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0081985", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"25", "xml_views"=>"3", "html_views"=>"67"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"88"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"72"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"85"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"84"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"59"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"35"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"41"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"35"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"27"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"47"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"36"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"34"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"9"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"13"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"50"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"8"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"4", "html_views"=>"7"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"6"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"16"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1311730"], "description"=>"<p>A ClustalW comparison was refined manually based on X-ray crystallographic structures of F<sub>420</sub>-dependent glucose-6-phosphate dehydrogenase (Fgd1) of <i>Mycobacterium tuberculosis</i>, F<sub>420</sub>-dependent methylenetetrahydromethanopterin reductase from <i>Methanopyrus kandleri</i> (MkMer), and F<sub>420</sub>-dependent secondary alcohol dehydrogenase (Adf) from <i>Methanoculleus thermophilicus </i><a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081985#pone.0081985-Bashiri1\" target=\"_blank\">[32]</a>, <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081985#pone.0081985-Shima1\" target=\"_blank\">[39]</a>, <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081985#pone.0081985-Aufhammer1\" target=\"_blank\">[40]</a>, <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081985#pone.0081985-Aufhammer2\" target=\"_blank\">[68]</a>. Residue labels: yellow shade and underlined, determined F<sub>420</sub>-binding residue; yellow shade, predicted F<sub>420</sub>-intercating residue; turquoise shade, forming positively charged pocket for binding the phosphate of glucose-6-phopsphate in Fgd1; green shade, residue involved in binding a citrate ion <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081985#pone.0081985-Bashiri1\" target=\"_blank\">[32]</a>.</p>", "links"=>[], "tags"=>["structures", "rv0132c", "mer", "mkmer"], "article_id"=>875162, "categories"=>["Biological Sciences"], "users"=>["Endang Purwantini", "Biswarup Mukhopadhyay"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0081985.g002", "stats"=>{"downloads"=>0, "page_views"=>22, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comparison_of_the_primary_structures_of_Rv0132c_fHMAD_and_three_Mer_homologs_Fgd1_MkMer_and_Adf_/875162", "title"=>"Comparison of the primary structures of Rv0132c (fHMAD) and three Mer homologs, Fgd1, MkMer and Adf.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-11 04:01:47"}
  • {"files"=>["https://ndownloader.figshare.com/files/1311738"], "description"=>"<p>Wild type (wt) and <i>ΔfbiC</i> strains of <i>M. smegmatis</i> carrying the indicated plasmids were analyzed (lane label, name of plasmid): None, no plasmid; pSMT3, pSMT3 (vector control); <i>hma</i>, pEP-hma; <i>rv0132c</i>, pEP-rv0132c; <i>rv0132c/hma</i>, pEP-rv0132c/hma. (+) and (-), cultivation of <i>M. smegmatis</i> (pEP-rv0132c/hma) with and without PA-824 (100 microgram per ml), respectively. Mycolic acid types: α, α′, epoxy (E), hydroxy (H), and keto (K) [<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081985#pone.0081985.s001\" target=\"_blank\">Fig. S1</a> shows the respective chemical structures.]. Panel A: <i>rv0132c</i> causing the conversion of H-MAs to K-MAs in wild-type <i>M. smegmatis</i>; Panel B: Requirement of <i>fbiC</i> for the production of K-MAs in <i>M. smegmatis</i> (pEP-rv0132c/hma) [Note: <i>The left most lane is for wt strain, used as control</i>]. Panel C: Inhibition of the production of K-MAs in <i>M. smegmatis</i> (pEP-rv0132c/hma) by PA-824.</p>", "links"=>[], "tags"=>["chromatography", "profiles", "methyl", "esters", "mycolic", "acids", "extracted", "strains", "grown"], "article_id"=>875170, "categories"=>["Biological Sciences"], "users"=>["Endang Purwantini", "Biswarup Mukhopadhyay"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0081985.g003", "stats"=>{"downloads"=>8, "page_views"=>237, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Thin_layer_chromatography_TLC_profiles_of_methyl_esters_of_mycolic_acids_extracted_from_various_Mycobacterium_smegmatis_strains_grown_in_the_absence_and_presence_of_PA_824_/875170", "title"=>"Thin layer chromatography (TLC) profiles of methyl esters of mycolic acids extracted from various <i>Mycobacterium smegmatis</i> strains grown in the absence and presence of PA-824.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-11 04:01:47"}
  • {"files"=>["https://ndownloader.figshare.com/files/1311758", "https://ndownloader.figshare.com/files/1311759", "https://ndownloader.figshare.com/files/1311760", "https://ndownloader.figshare.com/files/1311761"], "description"=>"<div><p>The ability of <i>Mycobacterium tuberculosis</i> to manipulate and evade human immune system is in part due to its extraordinarily complex cell wall. One of the key components of this cell wall is a family of lipids called mycolic acids. Oxygenation of mycolic acids generating methoxy- and ketomycolic acids enhances the pathogenic attributes of <i>M. tuberculosis</i>. Thus, the respective enzymes are of interest in the research on mycobacteria. The generation of methoxy- and ketomycolic acids proceeds through intermediary formation of hydroxymycolic acids. While the methyl transferase that generates methoxymycolic acids from hydroxymycolic acids is known, hydroxymycolic acids dehydrogenase that oxidizes hydroxymycolic acids to ketomycolic acids has been elusive. We found that hydroxymycolic acid dehydrogenase is encoded by the <i>rv0132c</i> gene and the enzyme utilizes F<sub>420</sub>, a deazaflavin coenzyme, as electron carrier, and accordingly we called it F<sub>420</sub>-dependent hydroxymycolic acid dehydrogenase. This is the first report on the involvement of F<sub>420</sub> in the synthesis of a mycobacterial cell envelope. Also, F<sub>420</sub>-dependent hydroxymycolic acid dehydrogenase was inhibited by PA-824, and therefore, it is a previously unknown target for this new tuberculosis drug.</p></div>", "links"=>[], "tags"=>["rv0132c", "encodes", "coenzyme", "hydroxymycolic", "dehydrogenase"], "article_id"=>875184, "categories"=>["Biological Sciences"], "users"=>["Endang Purwantini", "Biswarup Mukhopadhyay"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0081985.s001", "https://dx.doi.org/10.1371/journal.pone.0081985.s002", "https://dx.doi.org/10.1371/journal.pone.0081985.s003", "https://dx.doi.org/10.1371/journal.pone.0081985.s004"], "stats"=>{"downloads"=>9, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Rv0132c_of_Mycobacterium_tuberculosis_Encodes_a_Coenzyme_F_420_Dependent_Hydroxymycolic_Acid_Dehydrogenase/875184", "title"=>"Rv0132c of<i>Mycobacterium tuberculosis</i> Encodes a Coenzyme F<sub>420</sub>-Dependent Hydroxymycolic Acid Dehydrogenase", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-12-11 04:01:47"}
  • {"files"=>["https://ndownloader.figshare.com/files/1311753"], "description"=>"<p>The sources of hydroxymycolic acids or H-MAs (A) and Ketomycolic acids or K-MAs (B) were same as that indicated in the legend of <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081985#pone-0081985-g004\" target=\"_blank\">Fig. 4</a>. Epoxymycolic acids or E-MAs (C) were from lane “None” in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081985#pone-0081985-g003\" target=\"_blank\">Fig. 3</a>. In each case the inset shows expansion of the relevant regions.</p>", "links"=>[], "tags"=>["nmr", "spectra", "methyl", "esters", "mycolic", "acids", "recovered", "engineered"], "article_id"=>875179, "categories"=>["Biological Sciences"], "users"=>["Endang Purwantini", "Biswarup Mukhopadhyay"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0081985.g005", "stats"=>{"downloads"=>2, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Proton_NMR_spectra_of_methyl_esters_of_mycolic_acids_recovered_from_engineered_Mycobacterium_smegmatis_strains_/875179", "title"=>"Proton NMR spectra of methyl esters of mycolic acids recovered from engineered <i>Mycobacterium smegmatis</i> strains.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-11 04:01:47"}
  • {"files"=>["https://ndownloader.figshare.com/files/1311728"], "description"=>"<p>A common intermediate for various R groups is used as the starting point. Where MmaA2 and CmaA2 are involved in the formation of <i>cis</i> cyclopropane group, CmaA2 and an yet to identified enzyme (indicated by?) catalyze trans-cyclopropanation <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081985#pone.0081985-Barkan1\" target=\"_blank\">[48]</a>, <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081985#pone.0081985-Glickman1\" target=\"_blank\">[67]</a>. The details of the individual R groups are shown in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081985#pone.0081985.s001\" target=\"_blank\">Fig. S1</a>. * indicates that it is not known whether the cyclopropanation step follows or precedes oxygenation. All protons (except for the isolated groups) that have been target for NMR data analysis have been shown in red. The OH group shown in italics and underlined in the box at the left corner of the figure was converted to a methoxy group during saponification of mycolic acids; the process generated mycolic acids methyl esters (MAMEs).</p>", "links"=>[], "tags"=>["pathways", "synthesis", "methoxy-", "epoxymycolic", "acids", "mycobacteria"], "article_id"=>875160, "categories"=>["Biological Sciences"], "users"=>["Endang Purwantini", "Biswarup Mukhopadhyay"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0081985.g001", "stats"=>{"downloads"=>0, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Proposed_pathways_for_the_synthesis_of_hydroxy_keto_methoxy_and_epoxymycolic_acids_in_mycobacteria_7_24_/875160", "title"=>"Proposed pathways for the synthesis of hydroxy-, keto-, methoxy- and epoxymycolic acids in mycobacteria [7], [24].", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-11 04:01:47"}
  • {"files"=>["https://ndownloader.figshare.com/files/1311744"], "description"=>"<p>Hydroxymycolic acids were obtained from the lane <i>hma</i> and ketomycolic acids were from lane <i>rv0132c/hma</i> (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081985#pone-0081985-g003\" target=\"_blank\">Fig. 3A</a>). Only a part of each spectrum is shown and the annotations for the ion masses are based on reference <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081985#pone.0081985-Dinadayala1\" target=\"_blank\">[23]</a>: labels H & K, ions from hydroxy- and ketomycolic acids preparations; numbers 77–82: total number of carbon atoms in free acids. The unlabeled peaks belong to unidentified species that were present in both preparations.</p>", "links"=>[], "tags"=>["spectra", "methyl", "esters", "hydroxymyoclic", "acids", "ketomycolic", "recovered", "engineered"], "article_id"=>875172, "categories"=>["Biological Sciences"], "users"=>["Endang Purwantini", "Biswarup Mukhopadhyay"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0081985.g004", "stats"=>{"downloads"=>1, "page_views"=>25, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_MALDI_TOF_mass_spectra_of_methyl_esters_of_hydroxymyoclic_acids_A_and_ketomycolic_acids_B_recovered_from_engineered_Mycobacterium_smegmatis_strains_/875172", "title"=>"MALDI-TOF mass spectra of methyl esters of hydroxymyoclic acids (A) and ketomycolic acids (B) recovered from engineered <i>Mycobacterium smegmatis</i> strains.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-11 04:01:47"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"14", "full-text"=>"21", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"34", "full-text"=>"34", "pdf"=>"15", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"10", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"14", "full-text"=>"10", "pdf"=>"6", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"16", "full-text"=>"19", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"14", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"6", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"2", "pdf"=>"3", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"17", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"20", "full-text"=>"8", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"2", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"1", "full-text"=>"0", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"17", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"6", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}

Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[269, 466, 588, 697, 800, 896, 988, 1076, 1165, 1254, 1340, 1417]}, {"subject_area"=>"/Biology and life sciences/Mycology", "average_usage"=>[318, 546, 681, 824, 962, 1073, 1181, 1310, 1465, 1570, 1678, 1771, 1864]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[264, 460, 584, 692, 794, 887, 978, 1067, 1154, 1241, 1328, 1408, 1474]}, {"subject_area"=>"/Medicine and health sciences/Infectious diseases", "average_usage"=>[297, 523, 655, 765, 866, 971, 1070, 1159, 1256, 1337, 1424, 1496, 1568]}, {"subject_area"=>"/Physical sciences/Chemistry", "average_usage"=>[247, 429, 544, 647, 747, 842, 929, 1012, 1099, 1179, 1263, 1339, 1409]}]}
Loading … Spinner

Net::HTTPTooManyRequests

Source
Scopus
Time
2019-08-27 18:09:13 UTC
Target URL
https://api.elsevier.com/content/search/index:SCOPUS?query=DOI(10.1371%2Fjournal.pone.0081985)
Trace

/app/models/concerns/networkable.rb:21:in `get_result'
/app/models/source.rb:165:in `get_data'
/app/models/retrieval_status.rb:47:in `perform_get_data'
/app/jobs/source_job.rb:52:in `block (2 levels) in perform'
/app/jobs/source_job.rb:51:in `block in perform'
/app/jobs/source_job.rb:35:in `each'
/app/jobs/source_job.rb:35:in `perform'