The Relationship between Membrane Potential and Calcium Dynamics in Glucose-Stimulated Beta Cell Syncytium in Acute Mouse Pancreas Tissue Slices
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"The relationship between membrane potential and calcium dynamics in glucose-stimulated beta cell syncytium in acute mouse pancreas tissue slices", "type"=>"journal", "authors"=>[{"first_name"=>"Jurij", "last_name"=>"Dolenšek", "scopus_author_id"=>"12752912000"}, {"first_name"=>"Andraž", "last_name"=>"Stožer", "scopus_author_id"=>"35590650000"}, {"first_name"=>"Maša Skelin", "last_name"=>"Klemen", "scopus_author_id"=>"55912962800"}, {"first_name"=>"Evan W.", "last_name"=>"Miller", "scopus_author_id"=>"9734156100"}, {"first_name"=>"Marjan Slak", "last_name"=>"Rupnik", "scopus_author_id"=>"56074109100"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84891944010", "sgr"=>"84891944010", "pui"=>"372094675", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "pmid"=>"24324777", "doi"=>"10.1371/journal.pone.0082374"}, "id"=>"0e61d398-ec82-3f7f-91cf-05e6e0674233", "abstract"=>"Oscillatory electrical activity is regarded as a hallmark of the pancreatic beta cell glucose-dependent excitability pattern. Electrophysiologically recorded membrane potential oscillations in beta cells are associated with in-phase oscillatory cytosolic calcium activity ([Ca(2+)]i) measured with fluorescent probes. Recent high spatial and temporal resolution confocal imaging revealed that glucose stimulation of beta cells in intact islets within acute tissue slices produces a [Ca(2+)]i change with initial transient phase followed by a plateau phase with highly synchronized [Ca(2+)]i oscillations. Here, we aimed to correlate the plateau [Ca(2+)]i oscillations with the oscillations of membrane potential using patch-clamp and for the first time high resolution voltage-sensitive dye based confocal imaging. Our results demonstrated that the glucose-evoked membrane potential oscillations spread over the islet in a wave-like manner, their durations and wave velocities being comparable to the ones for [Ca(2+)]i oscillations and waves. High temporal resolution simultaneous records of membrane potential and [Ca(2+)]i confirmed tight but nevertheless limited coupling of the two processes, with membrane depolarization preceding the [Ca(2+)]i increase. The potassium channel blocker tetraethylammonium increased the velocity at which oscillations advanced over the islet by several-fold while, at the same time, emphasized differences in kinetics of the membrane potential and the [Ca(2+)]i. The combination of both imaging techniques provides a powerful tool that will help us attain deeper knowledge of the beta cell network.", "link"=>"http://www.mendeley.com/research/relationship-between-membrane-potential-calcium-dynamics-glucosestimulated-beta-cell-syncytium-acute", "reader_count"=>26, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Librarian"=>1, "Researcher"=>6, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>3, "Student > Master"=>3, "Other"=>1, "Professor"=>2}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Librarian"=>1, "Researcher"=>6, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>3, "Student > Master"=>3, "Other"=>1, "Professor"=>2}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Unspecified"=>1, "Biochemistry, Genetics and Molecular Biology"=>3, "Materials Science"=>1, "Mathematics"=>1, "Agricultural and Biological Sciences"=>9, "Medicine and Dentistry"=>3, "Philosophy"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>1, "Physics and Astronomy"=>2, "Chemistry"=>2, "Linguistics"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Chemistry"=>{"Chemistry"=>2}, "Physics and Astronomy"=>{"Physics and Astronomy"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>9}, "Linguistics"=>{"Linguistics"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Mathematics"=>{"Mathematics"=>1}, "Unspecified"=>{"Unspecified"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "Philosophy"=>{"Philosophy"=>1}}, "reader_count_by_country"=>{"Germany"=>1}, "group_count"=>1}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84891944010"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84891944010?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84891944010&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84891944010&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84891944010", "dc:identifier"=>"SCOPUS_ID:84891944010", "eid"=>"2-s2.0-84891944010", "dc:title"=>"The relationship between membrane potential and calcium dynamics in glucose-stimulated beta cell syncytium in acute mouse pancreas tissue slices", "dc:creator"=>"Dolenšek J.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"8", "prism:issueIdentifier"=>"12", "prism:pageRange"=>nil, "prism:coverDate"=>"2013-12-06", "prism:coverDisplayDate"=>"6 December 2013", "prism:doi"=>"10.1371/journal.pone.0082374", "citedby-count"=>"26", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Univerza v Mariboru", "affiliation-city"=>"Maribor", "affiliation-country"=>"Slovenia"}], "pubmed-id"=>"24324777", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e82374", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0082374", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"12", "year"=>"2013", "pdf_views"=>"56", "xml_views"=>"8", "html_views"=>"459"}
  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"26", "xml_views"=>"0", "html_views"=>"185"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"124"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"2", "html_views"=>"136"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"19", "xml_views"=>"2", "html_views"=>"101"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"85"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"68"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"80"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"43"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"38"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"41"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"33"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"77"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"42"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"65"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"57"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"114"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"45"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"44"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"66"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"2", "html_views"=>"33"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"44"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"88"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"29"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"4", "html_views"=>"23"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"39"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"12"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1306978"], "description"=>"<p><b>A</b> The voltage sensitive dye VF (upper panel) stained mostly cell membranes, whereas the calcium indicator Rhod-2 (middle panel) stained cytoplasms. For the active cells no significant co-localization was observed (lower panel). <b>B</b> Oscillation in the cell indicated with red asterisk in A during stimulation with 12 mM glucose plus 10 mM TEA. Arrows indicate the widths of oscillations at their half-maximal amplitude. Y axis represents the normalized fraction of the difference between maximum and plateau baseline fluorescence. <b>C</b> The delay between start<sub>50</sub> of the Rhod-2 and VF signals (1<sup>st</sup> quartile=13 ms, median=20 ms, 3<sup>rd</sup> quartile=36 ms, n=11 cells from 4 islets) was statistically significantly shorter than the delay between end<sub>50</sub> of the Rhod-2 and VF signals (1<sup>st</sup> quartile=150 ms, median=212 ms, 3<sup>rd</sup> quartile=239 ms, n=11 cells from 4 islets). Asterisks indicate p<0.001 (Mann-Whitney test). Resolution was 128x64 pixels at 170 Hz.</p>", "links"=>[], "tags"=>["membrane", "oscillations", "stimulation", "12", "mm", "glucose", "10", "tetraethylammonium"], "article_id"=>871181, "categories"=>["Biological Sciences"], "users"=>["Jurij Dolenšek", "Andraž Stožer", "Maša Skelin Klemen", "Evan W. Miller", "Marjan Slak Rupnik"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0082374.g007", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Simultaneous_recording_of_Ca_2_i_and_membrane_potential_oscillations_during_stimulation_with_12_mM_glucose_plus_10_mM_tetraethylammonium_TEA_/871181", "title"=>"Simultaneous recording of [Ca<sup>2+</sup>]<sub>i</sub> and membrane potential oscillations during stimulation with 12 mM glucose plus 10 mM tetraethylammonium (TEA).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-06 03:34:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/1306977"], "description"=>"<p><b>A</b> VF (left panel) stained mostly cell membranes while Rhod-2 (middle panel) stained cell cytoplasms, without any significant co-localization of the two dyes in the active regions (right panel; see also <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0082374#pone.0082374.s004\" target=\"_blank\">Figure S4</a>). <b>B</b> A color-coded representation of selected responsive cells with respect to the time lag between the oscillation start<sub>50</sub> of the first cell and every other cell for the VF signal (left panel), Rhod-2 signal (middle panel) and merge of the two (right panel). The outline stands for the VF signal and the interior for Rhod-2. Both membrane potential and [Ca<sup>2+</sup>]<sub>i</sub> wave roughly spread from top to bottom of the focal plane. Note that in any given cell, the membrane signal precedes (colder color) the [Ca<sup>2+</sup>]<sub>i</sub> signal, indicating that depolarization precedes the increase in [Ca<sup>2+</sup>]<sub>i</sub>. <b>C</b> Time traces of the VF and Rhod-2 signals from three cells indicated in the right panel of B show a wave-like spreading of both signals. In all cells, the VF signal clearly preceded the Rhod-2 signal. Arrows indicate the widths of oscillations at their half-maximal amplitude (see Methods). Y axis represents the normalized fraction of the difference between maximum and plateau baseline fluorescence. <b>D</b> Average trajectory for [Ca<sup>2+</sup>]<sub>i</sub> oscillation starts<sub>50</sub> (solid line) and ends<sub>50</sub> (dashed line). <b>E</b> Average trajectory for the membrane potential oscillation starts<sub>50</sub> (solid line) and ends<sub>50</sub> (dashed line). In D and E, numbers indicate angles in degrees. <b>F</b> Time lag between ends<sub>50</sub> (1<sup>st</sup> quartile=284 ms, median= 360 ms, 3<sup>rd</sup> quartile=473 ms) was significantly larger than the time lag between starts<sub>50</sub> (1<sup>st</sup> quartile=133 ms, median=170 ms, 3<sup>rd</sup> quartile=189 ms, n=21 cells) of the Rhod-2 and VF signals. Asterisks indicate p<0.001 (Mann-Whitney test). Resolution was 256x128 pixels at 52 Hz.</p>", "links"=>[], "tags"=>["membrane", "oscillations", "stimulation", "12", "mm"], "article_id"=>871180, "categories"=>["Biological Sciences"], "users"=>["Jurij Dolenšek", "Andraž Stožer", "Maša Skelin Klemen", "Evan W. Miller", "Marjan Slak Rupnik"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0082374.g006", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Simultaneous_recording_of_Ca_2_i_and_membrane_potential_oscillations_during_stimulation_with_12_mM_glucose_/871180", "title"=>"Simultaneous recording of [Ca<sup>2+</sup>]<sub>i</sub> and membrane potential oscillations during stimulation with 12 mM glucose.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-06 03:34:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/1306974"], "description"=>"<p><b>A</b> Durations of [Ca<sup>2+</sup>]<sub>i</sub> oscillations at their half-maximal amplitude in randomly picked cells from 7 islets assessed by OGB-1 (OGB-1, 1<sup>st</sup> quartile=1.4 s, median=1.9 s, 3<sup>rd</sup> quartile=2.2 s, n=7 islets), of membrane potential oscillations in randomly picked cells from 7 islets assessed by VF (VF, 1<sup>st</sup> quartile=1.8 s, median=2.4 s, 3<sup>rd</sup> quartile=3.2 s, n=7 islets) and of membrane potential oscillations assessed in 7 whole-cell patch-clamped cells (PATCH, 1<sup>st</sup> quartile=1.6 s, median=2.1 s, 3<sup>rd</sup> quartile=2.5 s, n=7 islets) are not statistically significantly different (Kruskal-Wallis test). <b>B</b> Median membrane potential wave velocities (VF: 1<sup>st</sup> quartile=55 µm/s, median=69 µm/s, 3<sup>rd</sup> quartile=97 µm/s, n=7) and [Ca<sup>2+</sup>]<sub>i</sub> wave velocities (OGB-1: 1<sup>st</sup> quartile=54 µm/s, median=89 µm/s, 3<sup>rd</sup> quartile=139 µm/s, n=6) were not statistically significantly different (Mann-Whitney test). </p>", "links"=>[], "tags"=>["oscillations", "velocities", "waves", "stimulation", "12", "mm", "glucose", "membrane"], "article_id"=>871177, "categories"=>["Biological Sciences"], "users"=>["Jurij Dolenšek", "Andraž Stožer", "Maša Skelin Klemen", "Evan W. Miller", "Marjan Slak Rupnik"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0082374.g004", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Durations_of_oscillations_and_velocities_of_waves_during_stimulation_with_12_mM_glucose_are_comparable_for_the_Ca_2_i_and_membrane_potential_signals_/871177", "title"=>"Durations of oscillations and velocities of waves during stimulation with 12 mM glucose are comparable for the [Ca<sup>2+</sup>]<sub>i</sub> and membrane potential signals.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-06 03:34:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/1306972"], "description"=>"<p><b>A</b> A high resolution image of an islet of Langerhans showing cells loaded with the Oregon Green 488 BAPTA-1 (OGB-1) Ca<sup>2+</sup> indicator. <b>B</b> Time lags between the start<sub>50</sub> of [Ca<sup>2+</sup>]<sub>i</sub> oscillation in the first and every other cell during stimulation with 12 mM glucose are color-coded. Note a wave-like spread of [Ca<sup>2+</sup>]<sub>i</sub> increase from left to right in this focal plane. <b>C</b> A single oscillation from three cells indicated in B during stimulation with 12 mM glucose. Gray rectangular areas indicate time intervals for increase and decrease of [Ca<sup>2+</sup>]<sub>i</sub> across different cells. Arrows indicate the duration of oscillations at their half-maximal amplitude. Y axis represents the normalized fraction of the difference between maximum and plateau baseline fluorescence. <b>D</b> A pseudo-colored representation of time lags between oscillation end<sub>50</sub> in the cell in which [Ca<sup>2+</sup>]<sub>i</sub> decreased first and every other cell. Note a wave-like spread of [Ca<sup>2+</sup>]<sub>i</sub> decrease from left to right in this focal plane. <b>E</b> Trajectories describing the wave path of the oscillation start<sub>50</sub> (blue, dashed line) and end<sub>50</sub> (red, dashed line). The trajectories describing average directions of spreading are drawn with solid lines. Note that the wave of [Ca<sup>2+</sup>]<sub>i</sub> decrease roughly follows the wave of [Ca<sup>2+</sup>]<sub>i</sub> increase. <b>F</b> Average wave trajectories of oscillation ends<sub>50</sub> (red) with respect to oscillation starts<sub>50</sub> (blue) for a single oscillation from 6 different islets of Langerhans. Numbers indicate angles in degrees. Note that the wave-fronts of ends<sub>50</sub> spread in roughly the same direction as the wave-fronts of starts<sub>50</sub> in most cases. Sampling rate was 50 Hz at a spatial resolution of 256x64 pixels.</p>", "links"=>[], "tags"=>["oscillations", "stimulation", "12", "mm"], "article_id"=>871175, "categories"=>["Biological Sciences"], "users"=>["Jurij Dolenšek", "Andraž Stožer", "Maša Skelin Klemen", "Evan W. Miller", "Marjan Slak Rupnik"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0082374.g002", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Ca_2_i_oscillations_during_stimulation_with_12_mM_glucose_/871175", "title"=>"[Ca<sup>2+</sup>]<sub>i</sub> oscillations during stimulation with 12 mM glucose.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-06 03:34:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/1306973"], "description"=>"<p><b>A</b> The VF dye labeled the majority of membranes in this islet of Langerhans from an acute mouse pancreas tissue slice. <b>B</b> During stimulation with 12 mM glucose, a wave of depolarization spread across the islet plane from left to right. Time lags between the start<sub>50</sub> of membrane potential oscillation in the first cell and the rest of the cells are color-coded. <b>C</b> The oscillatory behavior during stimulation consisted of depolarizations and repolarizations. A single wave is shown; numbers denote cells´ spatial positions indicated in A. Gray rectangular areas indicate time intervals for depolarizations and repolarizations across different cells. Arrows indicate the duration of oscillations at their half-maximal amplitudes. Y axis represents the normalized fraction of the difference between maximum and plateau baseline fluorescence. <b>D</b> In general, the spreading of ends<sub>50</sub> of oscillations did not take the same route as the starts<sub>50</sub> of membrane potential oscillations. Time lags are color-coded. <b>E</b> Trajectories (dashed lines) and the average trajectory (solid line) for the start<sub>50</sub> (blue) and end<sub>50</sub> (red) of the membrane potential oscillation. <b>D</b> Repolarization did not follow any clearly predictable pattern in the 6 islets. Shown are angles of average trajectories of oscillation ends<sub>50</sub> (red) relative to the respective starts<sub>50</sub> trajectory (blue). Sampling rate was 5 Hz at a spatial resolution of 512x256 pixels.</p>", "links"=>[], "tags"=>["oscillations", "stimulation", "12", "mm"], "article_id"=>871176, "categories"=>["Biological Sciences"], "users"=>["Jurij Dolenšek", "Andraž Stožer", "Maša Skelin Klemen", "Evan W. Miller", "Marjan Slak Rupnik"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0082374.g003", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Membrane_potential_oscillations_during_stimulation_with_12_mM_glucose_/871176", "title"=>"Membrane potential oscillations during stimulation with 12 mM glucose.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-06 03:34:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/1306971"], "description"=>"<p><b>A</b> [Ca<sup>2+</sup>]<sub>i</sub> oscillations were measured with a CCD camera at a temporal resolution of 2 Hz in cells in the tissue slice loaded with OGB-1. The patch-clamp pipette position is indicated with white lines. <b>B</b> During stimulation with 12 mM glucose, [Ca<sup>2+</sup>]<sub>i</sub> oscillations spread across the plane of the islet (from the lower right to the upper left corner in this figure). Responses of cells shown in A during a single [Ca<sup>2+</sup>]<sub>i</sub> oscillation are color-coded to represent time lags between the onset of [Ca<sup>2+</sup>]<sub>i</sub> increase in the cell where the visible part of the oscillation started and every other cell. Due to temporal resolution (2 Hz), cells were grouped into 4 groups only. The position of the patch-clamp pipette and the patch-clamped cell is indicated. The hatched pattern in the figure and legend bar indicates patch-clamp Data. <b>C</b> Two consecutive [Ca<sup>2+</sup>]<sub>i</sub> (upper panel) and membrane potential (lower panel) oscillations during stimulation with glucose. Each [Ca<sup>2+</sup>]<sub>i</sub> oscillation was accompanied by a membrane potential change consisting of a depolarization with superimposed spikes followed by a repolarization. Membrane potential was recorded at a temporal resolution of 1000 Hz. <b>D</b> A more detailed representation of the area indicated in C. The onset of high frequency spikes in the patched cell is temporally best correlated with the onset of [Ca<sup>2+</sup>]<sub>i</sub> oscillation in the group of cells indicated in orange. For this group of cells the distance to oscillation origin was roughly the same as for the patched cell. E Time delays between the first spike in the patch-clamped cell and the onset of [Ca<sup>2+</sup>]<sub>i</sub> oscillation in every other cell. Shortest time delays correspond to the orange group of cells for which the distance to wave origin was roughly the same as for the patch-clamped cell. Color coding as in B-D.</p>", "links"=>[], "tags"=>["membrane", "oscillations", "occurring", "patch-clamped", "cells", "islet"], "article_id"=>871174, "categories"=>["Biological Sciences"], "users"=>["Jurij Dolenšek", "Andraž Stožer", "Maša Skelin Klemen", "Evan W. Miller", "Marjan Slak Rupnik"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0082374.g001", "stats"=>{"downloads"=>0, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Simultaneous_recording_of_membrane_potential_oscillations_occurring_in_the_patch_clamped_cell_and_Ca_2_i_oscillations_occurring_in_other_cells_of_the_same_islet_of_Langerhans_/871174", "title"=>"Simultaneous recording of membrane potential oscillations occurring in the patch-clamped cell and [Ca<sup>2+</sup>]<sub>i</sub> oscillations occurring in other cells of the same islet of Langerhans.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-06 03:34:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/1306981", "https://ndownloader.figshare.com/files/1306982", "https://ndownloader.figshare.com/files/1306983", "https://ndownloader.figshare.com/files/1306984", "https://ndownloader.figshare.com/files/1306985", "https://ndownloader.figshare.com/files/1306986", "https://ndownloader.figshare.com/files/1306987"], "description"=>"<div><p>Oscillatory electrical activity is regarded as a hallmark of the pancreatic beta cell glucose-dependent excitability pattern. Electrophysiologically recorded membrane potential oscillations in beta cells are associated with in-phase oscillatory cytosolic calcium activity ([Ca<sup>2+</sup>]<sub>i</sub>) measured with fluorescent probes. Recent high spatial and temporal resolution confocal imaging revealed that glucose stimulation of beta cells in intact islets within acute tissue slices produces a [Ca<sup>2+</sup>]<sub>i</sub> change with initial transient phase followed by a plateau phase with highly synchronized [Ca<sup>2+</sup>]<sub>i</sub> oscillations. Here, we aimed to correlate the plateau [Ca<sup>2+</sup>]<sub>i</sub> oscillations with the oscillations of membrane potential using patch-clamp and for the first time high resolution voltage-sensitive dye based confocal imaging. Our results demonstrated that the glucose-evoked membrane potential oscillations spread over the islet in a wave-like manner, their durations and wave velocities being comparable to the ones for [Ca<sup>2+</sup>]<sub>i</sub> oscillations and waves. High temporal resolution simultaneous records of membrane potential and [Ca<sup>2+</sup>]<sub>i</sub> confirmed tight but nevertheless limited coupling of the two processes, with membrane depolarization preceding the [Ca<sup>2+</sup>]<sub>i</sub> increase. The potassium channel blocker tetraethylammonium increased the velocity at which oscillations advanced over the islet by several-fold while, at the same time, emphasized differences in kinetics of the membrane potential and the [Ca<sup>2+</sup>]<sub>i</sub>. The combination of both imaging techniques provides a powerful tool that will help us attain deeper knowledge of the beta cell network. </p> </div>", "links"=>[], "tags"=>["membrane", "calcium", "glucose-stimulated", "beta", "syncytium", "acute", "pancreas"], "article_id"=>871184, "categories"=>["Biological Sciences"], "users"=>["Jurij Dolenšek", "Andraž Stožer", "Maša Skelin Klemen", "Evan W. Miller", "Marjan Slak Rupnik"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0082374.s001", "https://dx.doi.org/10.1371/journal.pone.0082374.s002", "https://dx.doi.org/10.1371/journal.pone.0082374.s003", "https://dx.doi.org/10.1371/journal.pone.0082374.s004", "https://dx.doi.org/10.1371/journal.pone.0082374.s005", "https://dx.doi.org/10.1371/journal.pone.0082374.s006", "https://dx.doi.org/10.1371/journal.pone.0082374.s007"], "stats"=>{"downloads"=>1, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_Relationship_between_Membrane_Potential_and_Calcium_Dynamics_in_Glucose_Stimulated_Beta_Cell_Syncytium_in_Acute_Mouse_Pancreas_Tissue_Slices_/871184", "title"=>"The Relationship between Membrane Potential and Calcium Dynamics in Glucose-Stimulated Beta Cell Syncytium in Acute Mouse Pancreas Tissue Slices", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-12-06 03:34:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/1306975"], "description"=>"<p><b>A</b> A high resolution image showing loading of cells with the calcium-sensitive dye OGB-1. <b>B</b> A color-coded representation of responsive cells from A with respect to time lags between the start<sub>50</sub> of [Ca<sup>2+</sup>]<sub>i</sub> oscillation in the first cell and every other cell. <b>C</b> Time trace of a [Ca<sup>2+</sup>]<sub>i</sub> oscillation during stimulation with 12 mM glucose plus 10 mM TEA in the cell indicated in B. <b>D</b> A high resolution image showing labeling of cells with the voltage-sensitive dye VF. Note that almost exclusively plasma membranes are labeled. <b>E</b> A color-coded representation of responsive cells from D with respect to time lags between the start<sub>50</sub> of membrane potential oscillation in the first cell and every other cell. <b>F</b> Time trace of a membrane potential oscillation during stimulation with 12 mM glucose plus 10 mM TEA in the cell indicated in D. The time scale is the same for C and F. Y axis represents the normalized fraction of the difference between maximum and plateau baseline fluorescence. <b>G</b> Durations of [Ca<sup>2+</sup>]<sub>i</sub> oscillations (OGB-1: 1<sup>st</sup> quartile=135 ms, median=166 ms, 3<sup>rd</sup> quartile=248 ms, n=57 cells from three islets) and membrane potential oscillations (VF: 1<sup>st</sup> quartile=81 ms, median=101 ms, 3<sup>rd</sup> quartile= 162 ms, n=107 cells from five islets) at their half-maximal amplitude. Asterisks indicate a statistically significant difference (p<0.001, Mann-Whitney rank sum test). <b>H</b> Velocities of [Ca<sup>2+</sup>]<sub>i</sub> waves (OGB-1: 1<sup>st</sup> quartile=372 µm/s, median=623 µm/s, 3<sup>rd</sup> quartile=1058 µm/s, n=8 values from three islets) and membrane potential waves (VF: 1<sup>st</sup> quartile=457 µm/s, median=577 µm/s, 3<sup>rd</sup> quartile=857 µm/s, n=12 values from five islets) were not statistically significantly different. Resolution was 256 x 64 pixels at 97 Hz for OGB-1 and 256x128 at 99Hz for VF. </p>", "links"=>[], "tags"=>["membrane", "oscillations", "stimulation", "12", "mm", "glucose", "10", "tetraethylammonium"], "article_id"=>871178, "categories"=>["Biological Sciences"], "users"=>["Jurij Dolenšek", "Andraž Stožer", "Maša Skelin Klemen", "Evan W. Miller", "Marjan Slak Rupnik"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0082374.g005", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Ca_2_i_and_membrane_potential_oscillations_during_stimulation_with_12_mM_glucose_plus_10_mM_tetraethylammonium_TEA_/871178", "title"=>"[Ca<sup>2+</sup>]<sub>i</sub> and membrane potential oscillations during stimulation with 12 mM glucose plus 10 mM tetraethylammonium (TEA).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-06 03:34:02"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"16", "full-text"=>"19", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2013", "month"=>"12"}
  • {"unique-ip"=>"18", "full-text"=>"19", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"18", "full-text"=>"20", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"14", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"5", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"21", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"6", "cited-by"=>"1", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"24", "full-text"=>"26", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"8", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"10", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}

Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[269, 466, 588, 697, 800, 896, 988, 1076, 1165, 1254, 1340, 1417]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[272, 472, 600, 713, 815, 911, 1004, 1094, 1185, 1273, 1358, 1441]}, {"subject_area"=>"/Engineering and technology", "average_usage"=>[254, 440, 558, 675, 785, 883, 972, 1061, 1154, 1248, 1336, 1414, 1489]}, {"subject_area"=>"/Engineering and technology/Equipment", "average_usage"=>[246, 440, 558, 647, 746, 842, 937, 1028, 1116, 1191, 1253, 1348, 1420]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[264, 460, 584, 692, 794, 887, 978, 1067, 1154, 1241, 1328, 1408, 1474]}, {"subject_area"=>"/Physical sciences", "average_usage"=>[254, 431, 547, 651, 748, 842, 932, 1017, 1098, 1178, 1259, 1336, 1404]}, {"subject_area"=>"/Physical sciences/Chemistry", "average_usage"=>[247, 429, 544, 647, 747, 842, 929, 1012, 1099, 1179, 1263, 1339, 1409]}]}
Loading … Spinner
There are currently no alerts