Histone H4R3 Methylation Catalyzed by SKB1/PRMT5 Is Required for Maintaining Shoot Apical Meristem
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{"title"=>"Histone H4R3 methylation catalyzed by SKB1/PRMT5 is required for maintaining shoot apical meristem", "type"=>"journal", "authors"=>[{"first_name"=>"Minghui", "last_name"=>"Yue", "scopus_author_id"=>"43161486700"}, {"first_name"=>"Qiuling", "last_name"=>"Li", "scopus_author_id"=>"56006415900"}, {"first_name"=>"Ya", "last_name"=>"Zhang", "scopus_author_id"=>"37040988200"}, {"first_name"=>"Yan", "last_name"=>"Zhao", "scopus_author_id"=>"57188745090"}, {"first_name"=>"Zhaoliang", "last_name"=>"Zhang", "scopus_author_id"=>"43161561000"}, {"first_name"=>"Shilai", "last_name"=>"Bao", "scopus_author_id"=>"7201558796"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84892612604", "pmid"=>"24349476", "sgr"=>"84892612604", "doi"=>"10.1371/journal.pone.0083258", "isbn"=>"1932-6203", "issn"=>"19326203", "pui"=>"372134029"}, "id"=>"a6f5864a-45a2-3e0f-825c-fe7adac70236", "abstract"=>"The shoot apical meristem (SAM) is the source of all of the above-ground tissues and organs in post-embryonic development in higher plants. Studies have proven that the expression of genes constituting the WUSCHEL (WUS)-CLAVATA (CLV) feedback loop is critical for the SAM maintenance. Several histone lysine acetylation and methylation markers have been proven to regulate the transcription level of WUS. However, little is known about how histone arginine methylation regulates the expression of WUS and other genes. Here, we report that H4R3 symmetric dimethylation (H4R3sme2) mediated by SKB1/PRMT5 represses the expression of CORYNE (CRN) to maintain normal SAM geometrics. SKB1 lesion results in small SAM size in Arabidopsis, as well as down-regulated expression of WUS and CLV3. Up-regulation of WUS expression enlarges SAM size in skb1 mutant plants. We find that SKB1 and H4R3sme2 associate with the chromatin of the CRN locus to down-regulate its transcription. Mutation of CRN rescues the expression of WUS and the small SAM size of skb1. Thus, SKB1 and SKB1-mediated H4R3sme2 are required for the maintenance of SAM in Arabidopsis seedlings.", "link"=>"http://www.mendeley.com/research/histone-h4r3-methylation-catalyzed-skb1prmt5-required-maintaining-shoot-apical-meristem", "reader_count"=>32, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>7, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>12, "Student > Postgraduate"=>1, "Student > Master"=>4, "Other"=>1, "Student > Bachelor"=>2, "Professor"=>3}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>7, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>12, "Student > Postgraduate"=>1, "Student > Master"=>4, "Other"=>1, "Student > Bachelor"=>2, "Professor"=>3}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>4, "Agricultural and Biological Sciences"=>25, "Medicine and Dentistry"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>25}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"Republic of Singapore"=>1, "Canada"=>1}, "group_count"=>1}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1312908"], "description"=>"<div><p>(A) Comparison of seedling size of Col-0 and <i>skb1</i> grown on MS medium at 5 DAG and 10 DAG. Scale bar = 1 cm.</p>\n\t\t\t\t\t\t<p>(B) and (C) Comparison of rosette leaf growth rate of Col-0, <i>skb1</i> and 35S<i>::</i>SKB1 <i>skb1</i> plants grown on MS medium. Leaf initiation rate is calculatedas the number of new leaves produced per day between 6 DAG and 10 DAG. Data represent the means ± SE (n=36).</p>\n\t\t\t\t\t\t<p>(D) Comparison of SAM size of Col-0, <i>skb1</i> and 35S<i>::</i>SKB1 <i>skb1</i> plants grown on MS medium at 6 DAG. Scale bar = 20 μm.</p>\n\t\t\t\t\t\t<p>(E) Quantitative analysis of SAM size (upper panel) and Layer 1 cell number (lower panel) of Col-0 and <i>skb1</i> plants grown on MS medium at 6 DAG, 9 DAG and 12 DAG. L1 means layer 1 of SAM. More than 13 SAM sections were measured for size and counted for L1 cell number under microscope for each data point. Data represent the means ± SE.</p></div>", "links"=>[], "tags"=>["rates", "sam"], "article_id"=>876128, "categories"=>["Biological Sciences"], "users"=>["Minghui Yue", "Qiuling Li", "Ya Zhang", "Yan Zhao", "Zhaoliang Zhang", "Shilai Bao"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083258.g001", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Phenotypes_of_growth_rates_and_SAM_size_in_skb1_seedlings_/876128", "title"=>"Phenotypes of growth rates and SAM size in <i>skb1</i> seedlings.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-12 03:26:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1312914"], "description"=>"<div><p>(A) A diagram of the <i>CRN</i> gene structure, with bars representing the a-e regions examined by ChIP. The <i>CRN</i> open reading frame was shown by black boxes, while the exons of its neighbor genes (the left is At5G13300 and the right is At5G13280) were shown by white boxes.</p>\n\t\t\t\t\t\t<p>(B) The ChIP assay for <i>CRN</i> was performed with antibodies against SKB1 and H4R3sme2. Chromatin extracted from 12-day-old seedlings of Col-0 grown in soil, and <i>skb1</i> mutant chromatin as a control. Data represent triplicate quantitative real time PCR measurements of immunoprecipitated DNA, and the input represents chromatin before immunoprecipitation. Error bars represent relative SD of ChIP data.</p>\n\t\t\t\t\t\t<p>(C) Quantitative real-time-PCR analysis of the relative expression levels of <i>CRN</i> in Col-0, <i>skb1</i> and 35S<i>::</i>SKB1 <i>skb1</i> seedlings and normalized with ACTIN expression. Data represent the means ± SE of three independent experiments, <i>P</i> < 0.01.</p>\n\t\t\t\t\t\t<p>(D) and (E) Comparison of SAM size in Col-0, <i>skb1</i>, <i>crn</i>, and <i>crn </i><i>skb1</i> plants grown on MS medium to 9 DAG. Data in (E) represent the means ± SE (n≥11). Scale bar in (D) = 20 μm.</p>\n\t\t\t\t\t\t<p>(F) Staining results of GUS activity assays of pWUS<i>::GUS</i> and <i>pCLV3::GUS</i> transgenic lines in the Col-0, <i>skb1</i>, <i>crn</i>, and <i>crn </i><i>skb1</i> backgrounds, respectively. Materials used were seedlings grown to 9 DAG on MS medium. Scale bar = 20 μm.</p></div>", "links"=>[], "tags"=>["skb1", "regulating", "sam"], "article_id"=>876134, "categories"=>["Biological Sciences"], "users"=>["Minghui Yue", "Qiuling Li", "Ya Zhang", "Yan Zhao", "Zhaoliang Zhang", "Shilai Bao"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083258.g005", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_CRN_is_a_major_target_of_SKB1_in_regulating_SAM_size_/876134", "title"=>"CRN is a major target of SKB1 in regulating SAM size.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-12 03:26:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1312911"], "description"=>"<div><p>(A) Semi-quantitative RT–PCR analysis of known meristem regulators. TUBULIN (TUB) was the loading control. Three biological replicates were performed with similar results.</p>\n\t\t\t\t\t\t<p>(B) Quantitative real time PCR analysis of the expression of <i>WUS</i> and CLV3 in Col-0 and <i>skb1</i> seedlings and normalized with <i>ACTIN2</i> expression. Data represent the means ± SE of three independent experiments.</p>\n\t\t\t\t\t\t<p>(C) and (D) Staining results of GUS activity assays of pWUS<i>::GUS</i> (C) and <i>pCLV3::GUS</i> (D) transgenic lines in Col-0 and <i>skb1</i> backgrounds, respectively. Materials used were seedlings grown to 3, 6, 9 and 12 DAG on MS medium.</p>\n\t\t\t\t\t\t<p>(E) and (F) Staining results of GUS activity assays of pWUS<i>::GUS</i> (E) and <i>pCLV3::GUS</i> (F) transgenic lines in Col-0, <i>skb1</i>, <i>flc</i>, and <i>flc </i><i>skb1</i> backgrounds, respectively. Materials used were seedlings grown to 5 DAG on MS medium. Scale bar = 20 μm.</p></div>", "links"=>[], "tags"=>["profiles", "col-0"], "article_id"=>876131, "categories"=>["Biological Sciences"], "users"=>["Minghui Yue", "Qiuling Li", "Ya Zhang", "Yan Zhao", "Zhaoliang Zhang", "Shilai Bao"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083258.g003", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Differences_in_expression_profiles_between_Col_0_and_skb1_plants_/876131", "title"=>"Differences in expression profiles between Col-0 and <i>skb1</i> plants.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-12 03:26:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1312953", "https://ndownloader.figshare.com/files/1312954", "https://ndownloader.figshare.com/files/1312955", "https://ndownloader.figshare.com/files/1312956", "https://ndownloader.figshare.com/files/1312957", "https://ndownloader.figshare.com/files/1312958", "https://ndownloader.figshare.com/files/1312960"], "description"=>"<div><p>The shoot apical meristem (SAM) is the source of all of the above-ground tissues and organs in post-embryonic development in higher plants. Studies have proven that the expression of genes constituting the WUSCHEL (WUS)-<i>CLAVATA</i> (<i>CLV</i>) feedback loop is critical for the SAM maintenance. Several histone lysine acetylation and methylation markers have been proven to regulate the transcription level of <i>WUS</i>. However, little is known about how histone arginine methylation regulates the expression of <i>WUS</i> and other genes. Here, we report that H4R3 symmetric dimethylation (H4R3sme2) mediated by SKB1/PRMT5 represses the expression of <i>CORYNE</i> (<i>CRN</i>) to maintain normal SAM geometrics. <i>SKB1</i> lesion results in small SAM size in <i>Arabidopsis</i>, as well as down-regulated expression of <i>WUS</i> and <i>CLV3</i>. Up-regulation of <i>WUS</i> expression enlarges SAM size in <i>skb1</i> mutant plants. We find that SKB1 and H4R3sme2 associate with the chromatin of the <i>CRN</i> locus to down-regulate its transcription. Mutation of <i>CRN</i> rescues the expression of <i>WUS</i> and the small SAM size of <i>skb1</i>. Thus, <i>SKB1</i> and <i>SKB1</i>-mediated H4R3sme2 are required for the maintenance of SAM in <i>Arabidopsis</i> seedlings.</p> </div>", "links"=>[], "tags"=>["h4r3", "methylation", "catalyzed", "maintaining", "apical"], "article_id"=>876157, "categories"=>["Biological Sciences"], "users"=>["Minghui Yue", "Qiuling Li", "Ya Zhang", "Yan Zhao", "Zhaoliang Zhang", "Shilai Bao"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0083258.s001", "https://dx.doi.org/10.1371/journal.pone.0083258.s002", "https://dx.doi.org/10.1371/journal.pone.0083258.s003", "https://dx.doi.org/10.1371/journal.pone.0083258.s004", "https://dx.doi.org/10.1371/journal.pone.0083258.s005", "https://dx.doi.org/10.1371/journal.pone.0083258.s006", "https://dx.doi.org/10.1371/journal.pone.0083258.s007"], "stats"=>{"downloads"=>3, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Histone_H4R3_Methylation_Catalyzed_by_SKB1_PRMT5_Is_Required_for_Maintaining_Shoot_Apical_Meristem_/876157", "title"=>"Histone H4R3 Methylation Catalyzed by SKB1/PRMT5 Is Required for Maintaining Shoot Apical Meristem", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-12-12 03:26:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1312913"], "description"=>"<div><p>(A) Phenotype of <i>pga6</i> and <i>pga6 </i><i>skb1</i> with and without 17-β-estradiol treatment, grown on MS medium at 6 DAG. Scale bar = 1 cm. Estradiol+: treated with 17-β-estradiol; Estradiol-: no treated with 17-β-estradiol.</p>\n\t\t\t\t\t\t<p>(B) and (E) Quantitative real time PCR analysis of <i>WUS</i> (B) and CLV3 (E) expression in Col-0, <i>skb1</i>, <i>pga6</i> with 17-β-estradiol treatment, and <i>pga6 </i><i>skb1</i> with 17-β-estradiol treatment. Data represent the means± SE of three independent experiments.</p>\n\t\t\t\t\t\t<p>(C) and (D) Comparison of the SAM size in Col-0, <i>skb1</i>, and <i>pga6 </i><i>skb1</i> with 17-β-estradiol treatment. Data in (C) represent the means ± SE (n=12), <i>P</i> < 0.01. Scale bar in (D) = 20 μm.</p></div>", "links"=>[], "tags"=>["sam"], "article_id"=>876133, "categories"=>["Biological Sciences"], "users"=>["Minghui Yue", "Qiuling Li", "Ya Zhang", "Yan Zhao", "Zhaoliang Zhang", "Shilai Bao"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083258.g004", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Up_regulation_of_WUS_increases_the_SAM_size_of_skb1_/876133", "title"=>"Up-regulation of <i>WUS</i> increases the SAM size of <i>skb1</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-12 03:26:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1312909"], "description"=>"<div><p>(A) to (G) Comparison of the SAM size of L<i>er</i> (A), <i>skb1</i> (B), <i>clv1</i> (C), <i>clv1 </i><i>skb1</i> (D), <i>clv3</i> (E), <i>clv3 </i><i>skb1</i> (F) plants grown on MS medium at 9 DAG. Data in (G) represent the means ± SE (n≥12). Scale bar = 50 μm.</p>\n\t\t\t\t\t\t<p>(H) to (M) Top view of inflorescences of L<i>er</i> (H), <i>skb1</i> (I), <i>clv1</i> (J), <i>clv1 </i><i>skb1</i> (K), <i>clv3</i> (L), <i>clv3 </i><i>skb1</i> (M) at 5 weeks (H, J and L) or 7 weeks (I, K and M). Scale bar = 5 mm.</p>\n\t\t\t\t\t\t<p>(N) Comparison of the flowering time of L<i>er</i>, <i>skb1</i>, <i>clv1</i>, <i>clv1 </i><i>skb1</i>, <i>clv3</i> and <i>clv3 </i><i>skb1</i>. Bars represent mean values standard error deviation of rosette leaf number at bolting. For each line, 18 plants were scored.</p></div>", "links"=>[], "tags"=>[], "article_id"=>876129, "categories"=>["Biological Sciences"], "users"=>["Minghui Yue", "Qiuling Li", "Ya Zhang", "Yan Zhao", "Zhaoliang Zhang", "Shilai Bao"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083258.g002", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Phenotypes_of_clv1_skb1_and_clv3_skb1_double_mutants_/876129", "title"=>"Phenotypes of <i>clv1 skb1</i> and <i>clv3 skb1</i> double mutants.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-12 03:26:38"}

PMC Usage Stats | Further Information

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Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[269, 466, 588, 697, 800, 896, 988, 1076, 1165, 1254, 1340, 1417]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[272, 472, 600, 713, 815, 911, 1004, 1094, 1185, 1273, 1358, 1441]}, {"subject_area"=>"/Biology and life sciences/Organisms", "average_usage"=>[281, 484, 611, 728, 835, 934, 1030, 1123, 1214, 1299, 1383, 1464]}, {"subject_area"=>"/Biology and life sciences/Plant science", "average_usage"=>[269, 451, 577, 699, 812, 922, 1019, 1126, 1225, 1309, 1404, 1493, 1563]}]}
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