Targeted Expression of Suicide Gene by Tissue-Specific Promoter and MicroRNA Regulation for Cancer Gene Therapy
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{"title"=>"Targeted expression of suicide gene by tissue-specific promoter and microRNA regulation for cancer gene therapy", "type"=>"journal", "authors"=>[{"first_name"=>"Ravikanth", "last_name"=>"Danda", "scopus_author_id"=>"55191571500"}, {"first_name"=>"Gopinath", "last_name"=>"Krishnan", "scopus_author_id"=>"55353201900"}, {"first_name"=>"Kalaivani", "last_name"=>"Ganapathy", "scopus_author_id"=>"56079567800"}, {"first_name"=>"Uma Maheswari", "last_name"=>"Krishnan", "scopus_author_id"=>"35409613500"}, {"first_name"=>"Khetan", "last_name"=>"Vikas", "scopus_author_id"=>"6508284991"}, {"first_name"=>"Sailaja", "last_name"=>"Elchuri", "scopus_author_id"=>"8766793300"}, {"first_name"=>"Nivedita", "last_name"=>"Chatterjee", "scopus_author_id"=>"36934658900"}, {"first_name"=>"Subramanian", "last_name"=>"Krishnakumar", "scopus_author_id"=>"7004450099"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"372425283", "sgr"=>"84896726768", "issn"=>"19326203", "pmid"=>"24391761", "scopus"=>"2-s2.0-84896726768", "doi"=>"10.1371/journal.pone.0083398", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)"}, "id"=>"b3586f8a-1ff5-3df2-8277-690f74842fce", "abstract"=>"In order to realise the full potential of cancer suicide gene therapy that allows the precise expression of suicide gene in cancer cells, we used a tissue specific Epithelial cell adhesion molecule (EpCAM) promoter (EGP-2) that directs transgene Herpes simplex virus-thymidine kinase (HSV-TK) expression preferentially in EpCAM over expressing cancer cells. EpCAM levels are considerably higher in retinoblastoma (RB), a childhood eye cancer with limited expression in normal cells. Use of miRNA regulation, adjacent to the use of the tissue-specific promoter, would provide the second layer of control to the transgene expression only in the tumor cells while sparing the normal cells. To test this hypothesis we cloned let-7b miRNA targets in the 3'UTR region of HSV-TK suicide gene driven by EpCAM promoter because let-7 family miRNAs, including let-7b, were found to be down regulated in the RB tumors and cell lines. We used EpCAM over expressing and let-7 down regulated RB cell lines Y79, WERI-Rb1 (EpCAM (+ve)/let-7b(down-regulated)), EpCAM down regulated, let-7 over expressing normal retinal Müller glial cell line MIO-M1(EpCAM (-ve)/let-7b(up-regulated)), and EpCAM up regulated, let-7b up-regulated normal thyroid cell line N-Thy-Ori-3.1(EpCAM (+ve)/let-7b(up-regulated)) in the study. The cell proliferation was measured by MTT assay, apoptosis was measured by probing cleaved Caspase3, EpCAM and TK expression were quantified by Western blot. Our results showed that the EGP2-promoter HSV-TK (EGP2-TK) construct with 2 or 4 copies of let-7b miRNA targets expressed TK gene only in Y79, WERI-Rb-1, while the TK gene did not express in MIO-M1. In summary, we have developed a tissue-specific, miRNA-regulated dual control vector, which selectively expresses the suicide gene in EpCAM over expressing cells.", "link"=>"http://www.mendeley.com/research/targeted-expression-suicide-gene-tissuespecific-promoter-microrna-regulation-cancer-gene-therapy-1", "reader_count"=>38, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>2, "Student > Doctoral Student"=>2, "Researcher"=>7, "Student > Ph. D. Student"=>12, "Student > Postgraduate"=>1, "Student > Master"=>7, "Other"=>2, "Student > Bachelor"=>3, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>2, "Student > Doctoral Student"=>2, "Researcher"=>7, "Student > Ph. D. Student"=>12, "Student > Postgraduate"=>1, "Student > Master"=>7, "Other"=>2, "Student > Bachelor"=>3, "Lecturer"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>3, "Materials Science"=>1, "Agricultural and Biological Sciences"=>19, "Medicine and Dentistry"=>8, "Veterinary Science and Veterinary Medicine"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>3, "Computer Science"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>8}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>19}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Environmental Science"=>{"Environmental Science"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>3}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "reader_count_by_country"=>{"Argentina"=>1, "United States"=>1, "Egypt"=>1, "India"=>1}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1334633"], "description"=>"<p>miRNA target sequences two copies or four copies as detailed in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0083398#pone-0083398-t001\" target=\"_blank\">Table 1</a>. The reporter gene luciferase under CMV promoter was cloned in EpCAM-TK construct by removing TK gene and inserting luciferase gene. miRNA targets were cloned into 3′ UTR region of the expression vector containing Gluc gene under EpCAM promoter (A). The luciferase expression was quantified by secreted Gaussia luciferase. The plasmid constructs EGP2-luc, EGP2-luc-2T, EGP2-luc-4T, EGP2-luc-2C, and EGP2-luc-4C were transfected into MIO-M1, Nthy-ori 3-1, Y79, WERI-Rb1 cell lines. After incubation for 48 hrs the results were normalized to un-transfected cells and were expressed in relative light units (B). Luciferase levels are expressed as percentage mean ± S.D (n = 3). *p<0.05, **p<0.01, ***p<0.001 vs EGP-2-luc-2T.</p>", "links"=>[], "tags"=>["genetics", "gene expression", "RNA interference", "Obstetrics and gynecology", "breast cancer", "oncology", "Cancer treatment", "Gene therapy", "Cancers and neoplasms", "Ophthalmologic tumors", "retinoblastoma", "Basic cancer research", "Cancer prevention", "representations", "epcam", "promoter", "luciferase", "mirna", "targets", "vector", "constructs", "expressional", "studies"], "article_id"=>891008, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Ravikanth Danda", "Gopinath Krishnan", "Kalaivani Ganapathy", "Uma Maheswari Krishnan", "Khetan Vikas", "Sailaja Elchuri", "Nivedita Chatterjee", "Subramanian Krishnakumar"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083398.g004", "stats"=>{"downloads"=>0, "page_views"=>37, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Schematic_representations_of_EpCAM_promoter_with_luciferase_gene_and_miRNA_targets_vector_constructs_and_expressional_studies_of_the_constructs_using_luciferase_assay_/891008", "title"=>"Schematic representations of EpCAM promoter with luciferase gene and miRNA targets vector constructs and expressional studies of the constructs using luciferase assay.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-31 04:45:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1334636"], "description"=>"<p>Cell viability assay by MTT was evaluated by transfecting the EGP2-TK, EGP2-TK-2T, EGP2-TK-4T, EGP2-TK-2C, EGP2-TK-4C plasmids into the cell lines, Nthy-ori-3-1 (A), MIO-M1 (B), Y79 (C), WERI-Rb1 (D). After 48 hrs of transfection, followed by GCV treatment at different concentrations (1, 10, 50, 100 µM) for 24 hrs, readings were measured at 570 nm using spectrophotometer. The results were normalized to untransfected cells. Cell viability was shown as percentage mean ± S.D (n = 3). *p<0.05, **p<0.01, ***p<0.001 vs. control.</p>", "links"=>[], "tags"=>["genetics", "gene expression", "RNA interference", "Obstetrics and gynecology", "breast cancer", "oncology", "Cancer treatment", "Gene therapy", "Cancers and neoplasms", "Ophthalmologic tumors", "retinoblastoma", "Basic cancer research", "Cancer prevention", "lines", "regulated", "mirna"], "article_id"=>891011, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Ravikanth Danda", "Gopinath Krishnan", "Kalaivani Ganapathy", "Uma Maheswari Krishnan", "Khetan Vikas", "Sailaja Elchuri", "Nivedita Chatterjee", "Subramanian Krishnakumar"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083398.g005", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Targeted_cell_death_of_retinoblastoma_cell_lines_regulated_by_miRNA_targets_/891011", "title"=>"Targeted cell death of retinoblastoma cell lines regulated by miRNA targets.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-31 04:45:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1334622"], "description"=>"<p>EpCAM expression was evaluated by western blot analysis and β-actin was used as a loading control (A). The transgene expression driven by EGP2 and CMV promoter was evaluated by transient transfection of CMV-TK, EGP2-TK, CMV-luc, and EGP2-luc. After 2 days of transfection TK protein expression driven by CMV promoter was evaluated by western blot analysis and the results were normalized against the β-actin (B). TK protein expression driven by EGP2 promoter was evaluated by western blot analysis and results were normalized against the β-actin (C). Protein quantification was quantified by using ImageJ software and protein expression was expressed in relative signal intensity values. TK mRNA levels were quantified by Real-time PCR, the mRNA levels were normalized against the GAPDH and were expressed in relative fold change units (D). Luciferase expression analysis was performed by quantifying the secreted Gaussia luciferase. The results were normalized against un-transfected cells and were expressed in relative light units (E). Normalized levels of luciferase expression as percentage shown as mean ± S.D (n = 3). *p<0.05, **p<0.01, ***p<0.001 vs. Control, +p<0.05, ++p<0.01, +++p<0.001 vs. CMV-luc.</p>", "links"=>[], "tags"=>["genetics", "gene expression", "RNA interference", "Obstetrics and gynecology", "breast cancer", "oncology", "Cancer treatment", "Gene therapy", "Cancers and neoplasms", "Ophthalmologic tumors", "retinoblastoma", "Basic cancer research", "Cancer prevention", "promoter", "expressional", "studies", "transgene", "driven", "egp2", "cmv", "cancerous"], "article_id"=>890996, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Ravikanth Danda", "Gopinath Krishnan", "Kalaivani Ganapathy", "Uma Maheswari Krishnan", "Khetan Vikas", "Sailaja Elchuri", "Nivedita Chatterjee", "Subramanian Krishnakumar"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083398.g001", "stats"=>{"downloads"=>0, "page_views"=>28, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_In_vitro_promoter_strength_analysis_by_expressional_studies_of_transgene_driven_by_both_EGP2_and_CMV_promoter_in_cancerous_and_normal_cell_lines_/890996", "title"=>"<i>In vitro</i> promoter strength analysis by expressional studies of transgene driven by both EGP2 and CMV promoter in cancerous and normal cell lines.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-31 04:45:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1334652", "https://ndownloader.figshare.com/files/1334653"], "description"=>"<div><p>In order to realise the full potential of cancer suicide gene therapy that allows the precise expression of suicide gene in cancer cells, we used a tissue specific Epithelial cell adhesion molecule (EpCAM) promoter (EGP-2) that directs transgene Herpes simplex virus–thymidine kinase (HSV-TK) expression preferentially in EpCAM over expressing cancer cells. EpCAM levels are considerably higher in retinoblastoma (RB), a childhood eye cancer with limited expression in normal cells. Use of miRNA regulation, adjacent to the use of the tissue-specific promoter, would provide the second layer of control to the transgene expression only in the tumor cells while sparing the normal cells. To test this hypothesis we cloned let-7b miRNA targets in the 3’UTR region of HSV-TK suicide gene driven by EpCAM promoter because let-7 family miRNAs, including let-7b, were found to be down regulated in the RB tumors and cell lines. We used EpCAM over expressing and let-7 down regulated RB cell lines Y79, WERI-Rb1 (EpCAM<sup> +ve</sup>/let-7b<sup>down-regulated</sup>), EpCAM down regulated, let-7 over expressing normal retinal Müller glial cell line MIO-M1(EpCAM <sup>−ve</sup>/let-7b<sup>up-regulated</sup>), and EpCAM up regulated, let-7b up-regulated normal thyroid cell line N-Thy-Ori-3.1(EpCAM<sup> +ve</sup>/let-7b<sup>up-regulated</sup>) in the study. The cell proliferation was measured by MTT assay, apoptosis was measured by probing cleaved Caspase3, EpCAM and TK expression were quantified by Western blot. Our results showed that the EGP2-promoter HSV-TK (EGP2-TK) construct with 2 or 4 copies of let-7b miRNA targets expressed TK gene only in Y79, WERI-Rb-1, while the TK gene did not express in MIO-M1. In summary, we have developed a tissue-specific, miRNA-regulated dual control vector, which selectively expresses the suicide gene in EpCAM over expressing cells.</p></div>", "links"=>[], "tags"=>["genetics", "gene expression", "RNA interference", "Obstetrics and gynecology", "breast cancer", "oncology", "Cancer treatment", "Gene therapy", "Cancers and neoplasms", "Ophthalmologic tumors", "retinoblastoma", "Basic cancer research", "Cancer prevention", "tissue-specific", "promoter", "microrna", "cancer"], "article_id"=>891025, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Ravikanth Danda", "Gopinath Krishnan", "Kalaivani Ganapathy", "Uma Maheswari Krishnan", "Khetan Vikas", "Sailaja Elchuri", "Nivedita Chatterjee", "Subramanian Krishnakumar"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0083398.s001", "https://dx.doi.org/10.1371/journal.pone.0083398.s002"], "stats"=>{"downloads"=>8, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Targeted_Expression_of_Suicide_Gene_by_Tissue_Specific_Promoter_and_MicroRNA_Regulation_for_Cancer_Gene_Therapy_/891025", "title"=>"Targeted Expression of Suicide Gene by Tissue-Specific Promoter and MicroRNA Regulation for Cancer Gene Therapy", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2013-12-31 04:45:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1334650"], "description"=>"<p>Oligonucleotides used in this study.</p>", "links"=>[], "tags"=>["genetics", "gene expression", "RNA interference", "Obstetrics and gynecology", "breast cancer", "oncology", "Cancer treatment", "Gene therapy", "Cancers and neoplasms", "Ophthalmologic tumors", "retinoblastoma", "Basic cancer research", "Cancer prevention"], "article_id"=>891023, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Ravikanth Danda", "Gopinath Krishnan", "Kalaivani Ganapathy", "Uma Maheswari Krishnan", "Khetan Vikas", "Sailaja Elchuri", "Nivedita Chatterjee", "Subramanian Krishnakumar"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083398.t001", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Oligonucleotides_used_in_this_study_/891023", "title"=>"Oligonucleotides used in this study.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-12-31 04:45:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1334630"], "description"=>"<p>The expression of let-7 miRNAs were quantified using real time PCR. The expression analysis of let-7 family miRNAs was evaluated in retinoblastoma primary tumors. The results were normalized against normal adult retina and were expressed in fold change values relative to normal adult retina (A, B). The expression analysis of let-7 family miRNAs was evaluated in retinoblastoma cell lines. The results were normalized against MIO-M1 and were expressed in fold change values relative to MIO-M1(C). miRNA fold change was calculated using 2<sup>−▵▵CT</sup> method.</p>", "links"=>[], "tags"=>["genetics", "gene expression", "RNA interference", "Obstetrics and gynecology", "breast cancer", "oncology", "Cancer treatment", "Gene therapy", "Cancers and neoplasms", "Ophthalmologic tumors", "retinoblastoma", "Basic cancer research", "Cancer prevention", "mirna", "tumors"], "article_id"=>891005, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Ravikanth Danda", "Gopinath Krishnan", "Kalaivani Ganapathy", "Uma Maheswari Krishnan", "Khetan Vikas", "Sailaja Elchuri", "Nivedita Chatterjee", "Subramanian Krishnakumar"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083398.g003", "stats"=>{"downloads"=>4, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_let_7_miRNA_expression_analysis_in_Retinoblastoma_tumors_and_Retinoblastoma_cell_lines_/891005", "title"=>"let-7 miRNA expression analysis in Retinoblastoma tumors and Retinoblastoma cell lines.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-31 04:45:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1334649"], "description"=>"<p>In cancer cells EpCAM promoter mediated suicide gene expression with miRNA targets was high due to presence of cell specific expression of the promoter and lack of sufficient let-7 miRNA copies. Where as in Normal cells due to the presence of high copy number of let-7 miRNAs complementation to its target sequences which recruits RISC factor and degrades the mRNA resulting in “putative” translational repression of TK protein.</p>", "links"=>[], "tags"=>["genetics", "gene expression", "RNA interference", "Obstetrics and gynecology", "breast cancer", "oncology", "Cancer treatment", "Gene therapy", "Cancers and neoplasms", "Ophthalmologic tumors", "retinoblastoma", "Basic cancer research", "Cancer prevention", "epcam", "promoter", "mediated", "hsv-tk", "regulated", "let-7", "mirna"], "article_id"=>891022, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Ravikanth Danda", "Gopinath Krishnan", "Kalaivani Ganapathy", "Uma Maheswari Krishnan", "Khetan Vikas", "Sailaja Elchuri", "Nivedita Chatterjee", "Subramanian Krishnakumar"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083398.g008", "stats"=>{"downloads"=>1, "page_views"=>89, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Schematic_representation_of_EpCAM_promoter_mediated_HSV_TK_suicide_gene_expression_regulated_by_let_7_miRNA_targets_/891022", "title"=>"Schematic representation of EpCAM promoter mediated HSV-TK suicide gene expression regulated by let-7 miRNA targets.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-31 04:45:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1334646"], "description"=>"<p>Cell viability assay by MTT was evaluated by transfecting the EGP2-TK, EGP2-TK-2T, EGP2-TK-4T, EGP2-TK-2C, EGP2-TK-4C plasmids into the cell lines MDA-MB-453 (A) MCF7 (B). After 48 hrs of transfection, followed by GCV treatment at different concentrations (1, 10, 50, 100 µM) for 24 hrs. The results were normalized to un-transfected cells. Cell viability was shown as percentage mean ± S.D (n = 3). *p<0.05, **p<0.01, ***p<0.001 vs. control. The TK protein expression driven by EGP2 promoter and miRNA regulation was evaluated by transient transfection of EGP2-TK, EGP2-TK-2T, EGP2-TK-2C and treatment with GCV at 10 µM concentration for MDA-MB 453 and MCF7. After 48 hrs of transfection western blot analysis of TK protein expression normalized against the β-actin protein (C). Western blot analysis of apoptotic marker expression normalized against β-actin protein (D).</p>", "links"=>[], "tags"=>["genetics", "gene expression", "RNA interference", "Obstetrics and gynecology", "breast cancer", "oncology", "Cancer treatment", "Gene therapy", "Cancers and neoplasms", "Ophthalmologic tumors", "retinoblastoma", "Basic cancer research", "Cancer prevention", "apoptotic", "epcam", "promoter", "let-7b", "mirna", "targets", "mda-mb-453"], "article_id"=>891019, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Ravikanth Danda", "Gopinath Krishnan", "Kalaivani Ganapathy", "Uma Maheswari Krishnan", "Khetan Vikas", "Sailaja Elchuri", "Nivedita Chatterjee", "Subramanian Krishnakumar"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083398.g007", "stats"=>{"downloads"=>2, "page_views"=>68, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cytotoxicity_and_apoptotic_regulation_by_EpCAM_promoter_and_let_7b_miRNA_targets_in_MDA_MB_453_and_MCF7_/891019", "title"=>"Cytotoxicity and apoptotic regulation by EpCAM promoter and let-7b miRNA targets in MDA-MB-453 and MCF7.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-31 04:45:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1334624"], "description"=>"<p>Cell viability assay by MTT was evaluated by transfecting the EGP2-TK and CMV-TK plasmids into the cell lines. Nthy-ori-3-1 (A), MIO-M1 (B), Y79 (C), WERI-Rb1 (D). After 48 hrs of transfection, followed by GCV treatment at different concentrations (1, 10, 50, 100 µM) for 24 hrs. The results were normalized against un-transfected cells and were expressed in percentage of cell viability. Cell viability was shown as percentage mean ± S.D (n = 3).</p>", "links"=>[], "tags"=>["genetics", "gene expression", "RNA interference", "Obstetrics and gynecology", "breast cancer", "oncology", "Cancer treatment", "Gene therapy", "Cancers and neoplasms", "Ophthalmologic tumors", "retinoblastoma", "Basic cancer research", "Cancer prevention", "tk", "transfected"], "article_id"=>890999, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Ravikanth Danda", "Gopinath Krishnan", "Kalaivani Ganapathy", "Uma Maheswari Krishnan", "Khetan Vikas", "Sailaja Elchuri", "Nivedita Chatterjee", "Subramanian Krishnakumar"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083398.g002", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_GCV_sensitivity_to_TK_transfected_cells_/890999", "title"=>"GCV sensitivity to TK transfected cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-31 04:45:05"}
  • {"files"=>["https://ndownloader.figshare.com/files/1334638"], "description"=>"<p>Western blot analysis was performed for the presence of TK protein, EGP2-TK was transfected into Nthy-ori-3-1, MIO-M1, Y79 and WERI-Rb-1 cell lines (A). Western blot analysis was performed to assess the TK protein expression regulated under the influence of miRNA targets containing constructs, EGP2-TK-2T and EGP2-TK-2C were transfected into Nthy-ori-3-1, MIO-M1, Y79 and WERI-Rb-1 cell lines(B). To assess the apoptotic marker expression, transient transfection of,, EGP2-TK-2T, and GCV treatment for 48 hrs at 10 µM concentration in Nthy-ori-3-1, MIO-M1, Y79 and WERI-Rb-1cell lines was performed. Caspase and PARP expression was analysed by western blot. β-actin protein was used as loading control(C).</p>", "links"=>[], "tags"=>["genetics", "gene expression", "RNA interference", "Obstetrics and gynecology", "breast cancer", "oncology", "Cancer treatment", "Gene therapy", "Cancers and neoplasms", "Ophthalmologic tumors", "retinoblastoma", "Basic cancer research", "Cancer prevention", "tk", "apoptotic", "epcam", "promoter", "let-7b", "mirna"], "article_id"=>891013, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Ravikanth Danda", "Gopinath Krishnan", "Kalaivani Ganapathy", "Uma Maheswari Krishnan", "Khetan Vikas", "Sailaja Elchuri", "Nivedita Chatterjee", "Subramanian Krishnakumar"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083398.g006", "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Targeted_expression_of_TK_protein_and_apoptotic_regulation_by_EpCAM_promoter_and_let_7b_miRNA_targets_/891013", "title"=>"Targeted expression of TK protein and apoptotic regulation by EpCAM promoter and let-7b miRNA targets.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-31 04:45:05"}

PMC Usage Stats | Further Information

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Relative Metric

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