MiR-133b Is Down-Regulated in Human Osteosarcoma and Inhibits Osteosarcoma Cells Proliferation, Migration and Invasion, and Promotes Apoptosis
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{"title"=>"MiR-133b Is down-regulated in human osteosarcoma and inhibits osteosarcoma cells proliferation, migration and invasion, and promotes apoptosis", "type"=>"journal", "authors"=>[{"first_name"=>"Huafu", "last_name"=>"Zhao", "scopus_author_id"=>"57198971192"}, {"first_name"=>"Mei", "last_name"=>"Li", "scopus_author_id"=>"57199160674"}, {"first_name"=>"Lihua", "last_name"=>"Li", "scopus_author_id"=>"56041789500"}, {"first_name"=>"Xiaoming", "last_name"=>"Yang", "scopus_author_id"=>"57191642298"}, {"first_name"=>"Guobo", "last_name"=>"Lan", "scopus_author_id"=>"55613598400"}, {"first_name"=>"Yu", "last_name"=>"Zhang", "scopus_author_id"=>"55914959600"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "sgr"=>"84894261674", "scopus"=>"2-s2.0-84894261674", "pui"=>"372425310", "doi"=>"10.1371/journal.pone.0083571", "pmid"=>"24391788"}, "id"=>"f7c3a0b6-b4fc-33fc-90e5-8c89b79adb3b", "abstract"=>"MicroRNAs (miRNAs) decrease the expression of specific target oncogenes or tumor suppressor genes and thereby play crucial roles in tumorigenesis and tumor growth. To date, the potential miRNAs regulating osteosarcoma growth and progression are not fully identified yet. In this study, the miRNA microarray assay and hierarchical clustering analysis were performed in human osteosarcoma samples. In comparison with normal human skeletal muscle, 43 miRNAs were significantly differentially expressed in human osteosarcomas (fold change ≥2 and p≤0.05). Among these miRNAs, miR-133a and miR-133b expression was decreased by 135 folds and 47 folds respectively and the decreased expression was confirmed in both frozen and paraffin-embedded osteosarcoma samples. The miR-133b precursor expression vector was then transfected into osteosarcoma cell lines U2-OS and MG-63, and the stable transfectants were selected by puromycin. We found that stable over-expression of miR-133b in osteosarcoma cell lines U2-OS and MG-63 inhibited cell proliferation, invasion and migration, and induced apoptosis. Further, over-expression of miR-133b decreased the expression of predicted target genes BCL2L2, MCL-1, IGF1R and MET, as well as the expression of phospho-Akt and FAK. This study provides a new insight into miRNAs dysregulation in osteosarcoma, and indicates that miR-133b may play as a tumor suppressor gene in osteosarcoma.", "link"=>"http://www.mendeley.com/research/mir133b-downregulated-human-osteosarcoma-inhibits-osteosarcoma-cells-proliferation-migration-invasio-2", "reader_count"=>21, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Researcher"=>5, "Student > Ph. D. Student"=>3, "Other"=>2, "Student > Master"=>6, "Student > Bachelor"=>2, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Professor > Associate Professor"=>1, "Researcher"=>5, "Student > Ph. D. Student"=>3, "Other"=>2, "Student > Master"=>6, "Student > Bachelor"=>2, "Lecturer > Senior Lecturer"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>7, "Agricultural and Biological Sciences"=>7, "Medicine and Dentistry"=>5}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>5}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>7}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>7}, "Unspecified"=>{"Unspecified"=>2}}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1334457"], "description"=>"<p>(A) Over-expression of miR-133b in osteosarcoma cell lines U2-OS and MG-63 inhibited cell proliferation. U2-OS or MG-63 cells were transfected with either miR-133b precursor expression vector or pEGP-miR-null control vector, and stable clones were selected with puromycin. Compared with cells expressing miR-null, U2-OS cells with stable miR-133b expression exhibited decreased cell proliferation in 48, 72 and 96-hour incubation, while cell proliferation of MG-63 cells with stable miR-133b expression was decreased in 72 hours. Proliferation rate was normalized with absorbance value of non-treated U2-OS or MG-63 cells (<sup>*</sup>, <i>p</i>≤0.05; <sup>**</sup>, <i>p</i>≤0.01). (B) Over-expression of miR-133b in osteosarcoma cell lines U2-OS and MG-63 induced apoptosis. Cells were stained with Annexin V-PE and 7-AAD. Data were shown as mean ± SEM. (n = 3) and were representative of one of three independent experiments.</p>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "gene expression", "genetics", "Molecular cell biology", "Cell growth", "oncology", "Cancers and neoplasms", "Bone and soft tissue sarcomas", "osteosarcoma", "mir-133b", "proliferation", "apoptosis"], "article_id"=>890861, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Huafu Zhao", "Mei Li", "LiHua Li", "Xiaoming Yang", "Guobo Lan", "Yu Zhang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083571.g004", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Roles_of_miR_133b_in_cell_proliferation_and_apoptosis_of_osteosarcoma_cell_lines_/890861", "title"=>"Roles of miR-133b in cell proliferation and apoptosis of osteosarcoma cell lines.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-31 04:27:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/1334456"], "description"=>"<p>Paraffin-embedded human normal bone (P-NB) RNAs were used as controls. The decreased expression of miR-133a and miR-133b in paraffin-embedded osteosarcoma was consistent with those in frozen osteosarcoma samples (<sup>**</sup>, <i>p</i>≤0.01), whereas the increased expression of miR-645 was not obviously observed (<i>p</i>≥0.05).</p>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "gene expression", "genetics", "Molecular cell biology", "Cell growth", "oncology", "Cancers and neoplasms", "Bone and soft tissue sarcomas", "osteosarcoma", "mir-133b", "mir-645", "paraffin-embedded", "samples", "sybr"], "article_id"=>890860, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Huafu Zhao", "Mei Li", "LiHua Li", "Xiaoming Yang", "Guobo Lan", "Yu Zhang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083571.g003", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Confirmation_of_miR_133a_miR_133b_and_miR_645_expression_in_paraffin_embedded_human_osteosarcoma_samples_P_OS_using_SYBR_Green_qRT_PCR_/890860", "title"=>"Confirmation of miR-133a, miR-133b and miR-645 expression in paraffin-embedded human osteosarcoma samples (P-OS) using SYBR Green qRT-PCR.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-31 04:27:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/1334461"], "description"=>"<p>Abbreviations: OS, osteosarcoma; NM, normal muscle; N/A, not available.</p><p>, The number is the normalized expression of the microRNA, which reflects the relative abundance of miRNA in the samples.</p>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "gene expression", "genetics", "Molecular cell biology", "Cell growth", "oncology", "Cancers and neoplasms", "Bone and soft tissue sarcomas", "osteosarcoma", "mirnas"], "article_id"=>890865, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Huafu Zhao", "Mei Li", "LiHua Li", "Xiaoming Yang", "Guobo Lan", "Yu Zhang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083571.t002", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Normalized_expression_of_six_most_significantly_decreased_miRNAs_in_human_osteosarcomas_/890865", "title"=>"Normalized expression of six most significantly decreased miRNAs in human osteosarcomas.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-12-31 04:27:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/1334459"], "description"=>"<p>(A) BCL2L2, MCL-1, IGF1R and MET are predicted as the target genes of miR-133b. Location of predicted 3′UTR target sites for miR-133b in BCL2L2, MCL-1, IGF1R and MET is presented based on TargetScan 6.2. (B) Over-expression of miR-133b decreased the expression of BCL2L2, MCL-1, IGF1R, MET and FAK, and inhibited Akt phosphorylation in U2-OS and MG-63 cells. Expression of β-Tubulin was used as a control.</p>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "gene expression", "genetics", "Molecular cell biology", "Cell growth", "oncology", "Cancers and neoplasms", "Bone and soft tissue sarcomas", "osteosarcoma", "decreases", "phospho-akt"], "article_id"=>890863, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Huafu Zhao", "Mei Li", "LiHua Li", "Xiaoming Yang", "Guobo Lan", "Yu Zhang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083571.g006", "stats"=>{"downloads"=>2, "page_views"=>34, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_MiR_133b_decreases_the_expression_of_BCL2L2_MCL_1_IGF1R_MET_phospho_Akt_and_FAK_/890863", "title"=>"MiR-133b decreases the expression of BCL2L2, MCL-1, IGF1R, MET, phospho-Akt and FAK.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-31 04:27:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/1334460"], "description"=>"<p>Clinical characteristics of patients with high-grade osteosarcoma.</p>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "gene expression", "genetics", "Molecular cell biology", "Cell growth", "oncology", "Cancers and neoplasms", "Bone and soft tissue sarcomas", "osteosarcoma", "patients", "high-grade"], "article_id"=>890864, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Huafu Zhao", "Mei Li", "LiHua Li", "Xiaoming Yang", "Guobo Lan", "Yu Zhang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083571.t001", "stats"=>{"downloads"=>2, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Clinical_characteristics_of_patients_with_high_grade_osteosarcoma_/890864", "title"=>"Clinical characteristics of patients with high-grade osteosarcoma.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-12-31 04:27:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/1334458"], "description"=>"<p>(A) and (B), over-expression of miR-133b suppressed cell invasion and migration in U2-OS and MG-63 cells. Cell invasion and migration were visualized and examined by inverted phase contrast microscope (left, A and B; magnification: ×100). Compared to non-treated U2-OS cells or cells with miR-null expression, both percent invasion and percent migration of miR-133b over-expressed U2-OS and MG-63 cells were significantly decreased (<sup>*</sup>, <i>p</i>≤0.05; <sup>**</sup>, <i>p</i>≤0.01). The data were representative of one of three independent experiments.</p>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "gene expression", "genetics", "Molecular cell biology", "Cell growth", "oncology", "Cancers and neoplasms", "Bone and soft tissue sarcomas", "osteosarcoma", "mir-133b"], "article_id"=>890862, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Huafu Zhao", "Mei Li", "LiHua Li", "Xiaoming Yang", "Guobo Lan", "Yu Zhang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083571.g005", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Roles_of_miR_133b_in_invasion_and_migration_of_osteosarcoma_cell_lines_/890862", "title"=>"Roles of miR-133b in invasion and migration of osteosarcoma cell lines.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-31 04:27:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/1334455"], "description"=>"<p>Human normal muscle (NM) RNAs were used as controls. The average expression levels of miR-133a, miR-133b and miR-208b in osteosarcoma tissues were significantly down-regulated, while the average miR-645 expression was significantly up-regulated (<sup>**</sup>, <i>p</i>≤0.01).</p>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "gene expression", "genetics", "Molecular cell biology", "Cell growth", "oncology", "Cancers and neoplasms", "Bone and soft tissue sarcomas", "osteosarcoma", "differentially", "mirnas", "samples", "sybr"], "article_id"=>890859, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Huafu Zhao", "Mei Li", "LiHua Li", "Xiaoming Yang", "Guobo Lan", "Yu Zhang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083571.g002", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Relative_expression_of_top_eight_differentially_expressed_miRNAs_in_human_osteosarcoma_samples_OS_using_SYBR_Green_qRT_PCR_/890859", "title"=>"Relative expression of top eight differentially expressed miRNAs in human osteosarcoma samples (OS) using SYBR Green qRT-PCR.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-31 04:27:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/1334454"], "description"=>"<p>All of the miRNAs meet the criteria of volcano plot filtering (fold change ≥2.0 and <i>p</i>≤0.05). Red denotes high expression levels, whereas green depicts low-expression levels. Each listed miRNA is significantly differentially expressed (<i>p</i>≤0.05) between the osteosarcoma and normal muscle samples.</p>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "gene expression", "genetics", "Molecular cell biology", "Cell growth", "oncology", "Cancers and neoplasms", "Bone and soft tissue sarcomas", "osteosarcoma", "differentially", "mirnas"], "article_id"=>890858, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Huafu Zhao", "Mei Li", "LiHua Li", "Xiaoming Yang", "Guobo Lan", "Yu Zhang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083571.g001", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Hierarchical_cluster_analysis_of_differentially_expressed_miRNAs_in_human_osteosarcoma_samples_/890858", "title"=>"Hierarchical cluster analysis of differentially expressed miRNAs in human osteosarcoma samples.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-31 04:27:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/1334463"], "description"=>"<div><p>MicroRNAs (miRNAs) decrease the expression of specific target oncogenes or tumor suppressor genes and thereby play crucial roles in tumorigenesis and tumor growth. To date, the potential miRNAs regulating osteosarcoma growth and progression are not fully identified yet. In this study, the miRNA microarray assay and hierarchical clustering analysis were performed in human osteosarcoma samples. In comparison with normal human skeletal muscle, 43 miRNAs were significantly differentially expressed in human osteosarcomas (fold change ≥2 and <i>p</i>≤0.05). Among these miRNAs, miR-133a and miR-133b expression was decreased by 135 folds and 47 folds respectively and the decreased expression was confirmed in both frozen and paraffin-embedded osteosarcoma samples. The miR-133b precursor expression vector was then transfected into osteosarcoma cell lines U2-OS and MG-63, and the stable transfectants were selected by puromycin. We found that stable over-expression of miR-133b in osteosarcoma cell lines U2-OS and MG-63 inhibited cell proliferation, invasion and migration, and induced apoptosis. Further, over-expression of miR-133b decreased the expression of predicted target genes BCL2L2, MCL-1, IGF1R and MET, as well as the expression of phospho-Akt and FAK. This study provides a new insight into miRNAs dysregulation in osteosarcoma, and indicates that miR-133b may play as a tumor suppressor gene in osteosarcoma.</p></div>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "gene expression", "genetics", "Molecular cell biology", "Cell growth", "oncology", "Cancers and neoplasms", "Bone and soft tissue sarcomas", "osteosarcoma", "down-regulated", "inhibits", "cells"], "article_id"=>890867, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Huafu Zhao", "Mei Li", "LiHua Li", "Xiaoming Yang", "Guobo Lan", "Yu Zhang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083571", "stats"=>{"downloads"=>3, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_MiR_133b_Is_Down_Regulated_in_Human_Osteosarcoma_and_Inhibits_Osteosarcoma_Cells_Proliferation_Migration_and_Invasion_and_Promotes_Apoptosis_/890867", "title"=>"MiR-133b Is Down-Regulated in Human Osteosarcoma and Inhibits Osteosarcoma Cells Proliferation, Migration and Invasion, and Promotes Apoptosis", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-12-31 04:27:36"}
  • {"files"=>["https://ndownloader.figshare.com/files/1334462"], "description"=>"<p>Abbreviations: OS, osteosarcoma; NM, normal muscle.</p><p>, The number is the normalized expression of the microRNA, which reflects the relative abundance of miRNA in the samples.</p>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "gene expression", "genetics", "Molecular cell biology", "Cell growth", "oncology", "Cancers and neoplasms", "Bone and soft tissue sarcomas", "osteosarcoma", "mirnas"], "article_id"=>890866, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Huafu Zhao", "Mei Li", "LiHua Li", "Xiaoming Yang", "Guobo Lan", "Yu Zhang"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0083571.t003", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Normalized_expression_of_four_most_significantly_increased_miRNAs_in_human_osteosarcomas_/890866", "title"=>"Normalized expression of four most significantly increased miRNAs in human osteosarcomas.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2013-12-31 04:27:36"}

PMC Usage Stats | Further Information

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  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"11", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2013-01-01T00:00:00Z", "end_date"=>"2013-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[269, 466, 588, 697, 800, 896, 988, 1076, 1165, 1254, 1340, 1417]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[272, 472, 600, 713, 815, 911, 1004, 1094, 1185, 1273, 1358, 1441]}, {"subject_area"=>"/Biology and life sciences/Developmental biology", "average_usage"=>[275, 472, 605, 720, 822, 921, 1013, 1106, 1200, 1289, 1378, 1459, 1531]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[284, 491, 620, 738, 843, 945, 1043, 1137, 1225, 1315, 1400, 1479, 1555]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[272, 466, 589, 702, 806, 903, 995, 1086, 1176, 1258, 1347, 1422, 1493]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[264, 460, 584, 692, 794, 887, 978, 1067, 1154, 1241, 1328, 1408, 1474]}]}
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