Effect of Platelet Lysate on Human Cells Involved in Different Phases of Wound Healing
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{"title"=>"Effect of platelet lysate on human cells involved in different phases of wound healing", "type"=>"journal", "authors"=>[{"first_name"=>"Maria Chiara", "last_name"=>"Barsotti", "scopus_author_id"=>"6603946974"}, {"first_name"=>"Paola", "last_name"=>"Losi", "scopus_author_id"=>"16316684600"}, {"first_name"=>"Enrica", "last_name"=>"Briganti", "scopus_author_id"=>"16315012700"}, {"first_name"=>"Elena", "last_name"=>"Sanguinetti", "scopus_author_id"=>"55246333200"}, {"first_name"=>"Angela", "last_name"=>"Magera", "scopus_author_id"=>"35776643600"}, {"first_name"=>"Tamer", "last_name"=>"Al Kayal", "scopus_author_id"=>"37076550900"}, {"first_name"=>"Roberto", "last_name"=>"Feriani", "scopus_author_id"=>"55615165200"}, {"first_name"=>"Rossella", "last_name"=>"Di Stefano", "scopus_author_id"=>"7006588004"}, {"first_name"=>"Giorgio", "last_name"=>"Soldani", "scopus_author_id"=>"7005206730"}], "year"=>2013, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"372309705", "sgr"=>"84893528923", "issn"=>"19326203", "pmid"=>"24386412", "scopus"=>"2-s2.0-84893528923", "doi"=>"10.1371/journal.pone.0084753", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)"}, "id"=>"c186bc94-ff7e-35de-badb-ab33cc1233b9", "abstract"=>"BACKGROUND: Platelets are rich in mediators able to positively affect cell activity in wound healing. Aim of this study was to characterize the effect of different concentrations of human pooled allogeneic platelet lysate on human cells involved in the different phases of wound healing (inflammatory phase, angiogenesis, extracellular matrix secretion and epithelialization).\\n\\nMETHODOLOGY/PRINCIPAL FINDINGS: Platelet lysate effect was studied on endothelial cells, monocytes, fibroblasts and keratinocytes, in terms of viability and proliferation, migration, angiogenesis, tissue repair pathway activation (ERK1/2) and inflammatory response evaluation (NFκB). Results were compared both with basal medium and with a positive control containing serum and growth factors. Platelet lysate induced viability and proliferation at the highest concentrations tested (10% and 20% v/v). Whereas both platelet lysate concentrations increased cell migration, only 20% platelet lysate was able to significantly promote angiogenic activity (p<0.05 vs. control), comparably to the positive control. Both platelet lysate concentrations activated important inflammatory pathways such as ERK1/2 and NFκB with the same early kinetics, whereas the effect was different for later time-points.\\n\\nCONCLUSION/SIGNIFICANCE: These data suggest the possibility of using allogeneic platelet lysate as both an alternative to growth factors commonly used for cell culture and as a tool for clinical regenerative application for wound healing.", "link"=>"http://www.mendeley.com/research/effect-platelet-lysate-human-cells-involved-different-phases-wound-healing-4", "reader_count"=>42, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Researcher"=>11, "Student > Doctoral Student"=>5, "Student > Ph. D. Student"=>9, "Student > Postgraduate"=>1, "Student > Master"=>8, "Student > Bachelor"=>6}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Researcher"=>11, "Student > Doctoral Student"=>5, "Student > Ph. D. Student"=>9, "Student > Postgraduate"=>1, "Student > Master"=>8, "Student > Bachelor"=>6}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Engineering"=>3, "Biochemistry, Genetics and Molecular Biology"=>7, "Materials Science"=>1, "Medicine and Dentistry"=>12, "Agricultural and Biological Sciences"=>14, "Veterinary Science and Veterinary Medicine"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>3}, "Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>12}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>14}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>7}, "Unspecified"=>{"Unspecified"=>3}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "reader_count_by_country"=>{"Netherlands"=>1, "France"=>1}, "group_count"=>2}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1329988"], "description"=>"<p>The effect on HUVECs of 10% or 20% PL-pre-treatment in serum-free medium for 48 hours, followed by seeding on Matrigel, is shown. The following morphometric parameters were evaluated after 8 hours of angiogenesis assay by AngioJ analysis: area % (<b>A</b>), branching points (<b>B</b>), total tubule length (<b>C</b>). Data are expressed as mean±SD of three independent experiments carried out in duplicate. The 20% PL significantly increased morphometric parameters (p<0.05 vs. 0% PL) comparably to the complete medium. A non significant increase was observed with 10% PL.</p>", "links"=>[], "tags"=>[], "article_id"=>887610, "categories"=>["Biological Sciences"], "users"=>["Maria Chiara Barsotti", "Paola Losi", "Enrica Briganti", "Elena Sanguinetti", "Angela Magera", "Tamer Al Kayal", "Roberto Feriani", "Rossella Di Stefano", "Giorgio Soldani"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0084753.g005", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Angiogenesis_assay_/887610", "title"=>"Angiogenesis assay.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-27 02:55:33"}
  • {"files"=>["https://ndownloader.figshare.com/files/1329982"], "description"=>"<p>HUVEC viability (<b>A</b>) and proliferation (<b>B</b>) were assessed by XTT assay and BrdU incorporation assay, respectively, following 48 hours of incubation with different PL concentrations (1, 5, 10 and 20%). The percentage of cell viability and proliferation were calculated versus the complete medium (assumed as 100%). Data are means ± SD of values obtained from three independent experiments with six replicates each. * p<0.01 vs. serum-free medium, 1 and 5% PL. .</p>", "links"=>[], "tags"=>["viability"], "article_id"=>887604, "categories"=>["Biological Sciences"], "users"=>["Maria Chiara Barsotti", "Paola Losi", "Enrica Briganti", "Elena Sanguinetti", "Angela Magera", "Tamer Al Kayal", "Roberto Feriani", "Rossella Di Stefano", "Giorgio Soldani"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0084753.g001", "stats"=>{"downloads"=>1, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_HUVEC_viability_and_proliferation_/887604", "title"=>"HUVEC viability and proliferation.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-27 02:55:33"}
  • {"files"=>["https://ndownloader.figshare.com/files/1329990"], "description"=>"<p>Time-course of ERK1/2 and NFκB phosphorylation after 10% or 20% PL treatment of HUVECs. Data are expressed as mean±SD of the ratio between the absorbance referred to the phosphorylated form and to the corresponding total protein. (<b>A</b>) Phosphorylation of ERK1/2. *: p<0.05; **: p<0.005, all vs. 0% PL. #: p<0.05 vs. both 15 and 30 min. α: p<0.001 vs. complete medium. (<b>B</b>) Phosphorylation of NFκB at Ser 468. *: p<0.05; **: p<0.01, all vs. 0% PL. #: p<0.005 vs. both 1 and 24 hours. α: p<0.05; αα: p<0.01, all vs. complete medium. (<b>C</b>) Phosphorylation of NFκB at Ser 536. *: p<0.05; **: p<0.0001, all vs. 0%PL. &: p<0.05 vs. 10% PL. #: p<0.05; ##: p<0.005, all vs. 1 hour. °: p<0.01 vs. 24 hours. α: p<0.05 vs. complete medium.</p>", "links"=>[], "tags"=>["intracellular", "pathways", "inflammatory"], "article_id"=>887612, "categories"=>["Biological Sciences"], "users"=>["Maria Chiara Barsotti", "Paola Losi", "Enrica Briganti", "Elena Sanguinetti", "Angela Magera", "Tamer Al Kayal", "Roberto Feriani", "Rossella Di Stefano", "Giorgio Soldani"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0084753.g006", "stats"=>{"downloads"=>0, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Activation_of_intracellular_pathways_and_inflammatory_response_/887612", "title"=>"Activation of intracellular pathways and inflammatory response.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-27 02:55:33"}
  • {"files"=>["https://ndownloader.figshare.com/files/1329987"], "description"=>"<p>Quantification of the effect of PL on scratch closure. A progressive closure was observed with PL treatment, starting from 18 hours. Scratch wounded cells incubated with 10% and 20% PL for 48 and 72 hours showed a significant higher scratch closure as compared to 5% PL, comparable to complete medium (positive control). *: p<0.05; **: p<0.005, all vs. 0% PL. α: p<0.05 vs. 5% PL. Bars represent the means ± SD of values obtained from three independent experiments each one with three replicates per group of treatment. Closure rates are expressed as percentage of scratch closure after 6, 18, 24, 48 and 72 hours compared to the initial area.</p>", "links"=>[], "tags"=>["closure"], "article_id"=>887609, "categories"=>["Biological Sciences"], "users"=>["Maria Chiara Barsotti", "Paola Losi", "Enrica Briganti", "Elena Sanguinetti", "Angela Magera", "Tamer Al Kayal", "Roberto Feriani", "Rossella Di Stefano", "Giorgio Soldani"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0084753.g004", "stats"=>{"downloads"=>0, "page_views"=>23, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Scratch_closure_rate_/887609", "title"=>"Scratch closure rate.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-27 02:55:33"}
  • {"files"=>["https://ndownloader.figshare.com/files/1329985"], "description"=>"<p>Representative phase contrast micrographs of 0 and 20% PL-treated keratinocytes at 0, 24 and 72 hours are shown. Scale bar: 200 μm.</p>", "links"=>[], "tags"=>["closure"], "article_id"=>887607, "categories"=>["Biological Sciences"], "users"=>["Maria Chiara Barsotti", "Paola Losi", "Enrica Briganti", "Elena Sanguinetti", "Angela Magera", "Tamer Al Kayal", "Roberto Feriani", "Rossella Di Stefano", "Giorgio Soldani"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0084753.g003", "stats"=>{"downloads"=>4, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Scratch_closure_assay_/887607", "title"=>"Scratch closure assay.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-27 02:55:33"}
  • {"files"=>["https://ndownloader.figshare.com/files/1329984"], "description"=>"<p>The chemotactic effect of PL on monocytes was assessed following 2 hours of cell migration towards different PL concentrations (5, 10 and 20%). Positive and negative controls are represented by 10 ng/ml MCP-1 and by serum-free medium, respectively. Data (n=3) are means ± SD of six replicates. * p<0.05 vs. both serum-free medium and 5% PL. .</p>", "links"=>[], "tags"=>["chemotaxis"], "article_id"=>887606, "categories"=>["Biological Sciences"], "users"=>["Maria Chiara Barsotti", "Paola Losi", "Enrica Briganti", "Elena Sanguinetti", "Angela Magera", "Tamer Al Kayal", "Roberto Feriani", "Rossella Di Stefano", "Giorgio Soldani"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0084753.g002", "stats"=>{"downloads"=>1, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Monocyte_chemotaxis_assay_/887606", "title"=>"Monocyte chemotaxis assay.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2013-12-27 02:55:33"}

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Relative Metric

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