Correcting the Cystic Fibrosis Disease Mutant, A455E CFTR
Publication Date
January 08, 2014
Journal
PLOS ONE
Authors
Liudmila Cebotaru, Daniele Rapino, Valeriu Cebotaru & William B. Guggino
Volume
9
Issue
1
Pages
e85183
DOI
https://dx.plos.org/10.1371/journal.pone.0085183
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0085183
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/24416359
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3885674
Europe PMC
http://europepmc.org/abstract/MED/24416359
Web of Science
000329862500224
Scopus
84896913799
Mendeley
http://www.mendeley.com/research/correcting-cystic-fibrosis-disease-mutant-a455e-cftr
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Mendeley | Further Information

{"title"=>"Correcting the cystic fibrosis disease mutant, A455E CFTR", "type"=>"journal", "authors"=>[{"first_name"=>"Liudmila", "last_name"=>"Cebotaru", "scopus_author_id"=>"6507664281"}, {"first_name"=>"Daniele", "last_name"=>"Rapino", "scopus_author_id"=>"57127278200"}, {"first_name"=>"Valeriu", "last_name"=>"Cebotaru", "scopus_author_id"=>"7801665308"}, {"first_name"=>"William B.", "last_name"=>"Guggino", "scopus_author_id"=>"35448882600"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"372704118", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0085183", "scopus"=>"2-s2.0-84896913799", "pmid"=>"24416359", "sgr"=>"84896913799"}, "id"=>"0a468711-ea8e-337b-b45f-84f6dc5d14da", "abstract"=>"Cystic fibrosis is caused by more than 1000 mutations, the most common being the ΔF508 mutation. These mutations have been divided into five classes [1], with ΔF508 CFTR in class II. Here we have studied the class V mutation A455E. We report that the mature and immature bands of A455E are rapidly degraded primarily by proteasomes; the short protein half-life of this mutant therefore resembles that of ΔF508 CFTR. A455E could be rescued by treatment of the cells with proteasome inhibitors. Furthermore, co-transfection of A455E with the truncation mutant Δ264 CFTR also rescued the mature C band, indicating that A455E can be rescued by transcomplementation. We found that Δ264 CFTR bound to A455E, forming a bimolecular complex. Treatment with the compound correctors C3 and C4 also rescued A455E. These results are significant because they show that although ΔF508 belongs to a different class than A455E, it can be rescued by the same strategies, offering therapeutic promise to patients with Class V mutations.", "link"=>"http://www.mendeley.com/research/correcting-cystic-fibrosis-disease-mutant-a455e-cftr", "reader_count"=>28, "reader_count_by_academic_status"=>{"Librarian"=>1, "Researcher"=>7, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>4, "Student > Master"=>1, "Other"=>3, "Student > Bachelor"=>9, "Lecturer > Senior Lecturer"=>2}, "reader_count_by_user_role"=>{"Librarian"=>1, "Researcher"=>7, "Student > Doctoral Student"=>1, "Student > Ph. D. Student"=>4, "Student > Master"=>1, "Other"=>3, "Student > Bachelor"=>9, "Lecturer > Senior Lecturer"=>2}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>4, "Agricultural and Biological Sciences"=>12, "Medicine and Dentistry"=>8, "Arts and Humanities"=>1, "Pharmacology, Toxicology and Pharmaceutical Science"=>2, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>8}, "Chemistry"=>{"Chemistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>12}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>2}, "Arts and Humanities"=>{"Arts and Humanities"=>1}}, "reader_count_by_country"=>{"Canada"=>1, "Belgium"=>1, "India"=>1}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1341463"], "description"=>"<p>Cos7 cells were transfected with A455E CFTR cDNA and treated for 16 h with the lysosome inhibitor E64 (n = 4) (<b>A</b>) There was very little change in band density in any of the treated groups or in the presence of the inhibitors when compared to the control or the HDAC6 inhibitor tubacin to inhibit aggresomes (n = 3) (<b>B, C</b>). Note that there is a significant increase with the highest concentration used.</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "genetics", "Human genetics", "Autosomal recessive", "cystic fibrosis", "population genetics", "mutation", "Genetic mutation", "Genetics of disease", "Population biology", "Anatomy and physiology", "Clinical genetics", "Pulmonology"], "article_id"=>896448, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Liudmila Cebotaru", "Daniele Rapino", "Valeriu Cebotaru", "William B. Guggino"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0085183.g003", "stats"=>{"downloads"=>1, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Lysosome_aggresome_inhibition_/896448", "title"=>"Lysosome/aggresome inhibition.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-08 03:15:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/1341471"], "description"=>"<p>Cos7 cells were transfected with A455E cDNA and treated with cycloheximide (25 µg/ml) for the indicated times. Note the rapid decay of both the mature C and immature B bands of A455E. (n = 4).</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "genetics", "Human genetics", "Autosomal recessive", "cystic fibrosis", "population genetics", "mutation", "Genetic mutation", "Genetics of disease", "Population biology", "Anatomy and physiology", "Clinical genetics", "Pulmonology", "synthesis", "inhibition", "degradation"], "article_id"=>896450, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Liudmila Cebotaru", "Daniele Rapino", "Valeriu Cebotaru", "William B. Guggino"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0085183.g004", "stats"=>{"downloads"=>1, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_protein_synthesis_inhibition_on_the_degradation_of_A455E_/896450", "title"=>"Effect of protein synthesis inhibition on the degradation of A455E.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-08 03:15:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/1341482"], "description"=>"<p>. Both the C and B bands of A455E CFTR were increased when cells were cotransfected with Δ27-264 CFTR, showing that A455E could be rescued by transcomplementation. (n = 11).</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "genetics", "Human genetics", "Autosomal recessive", "cystic fibrosis", "population genetics", "mutation", "Genetic mutation", "Genetics of disease", "Population biology", "Anatomy and physiology", "Clinical genetics", "Pulmonology", "a455e"], "article_id"=>896451, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Liudmila Cebotaru", "Daniele Rapino", "Valeriu Cebotaru", "William B. Guggino"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0085183.g005", "stats"=>{"downloads"=>2, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transcomplementation_of_A455E_by_916_27_264_CFTR_/896451", "title"=>"Transcomplementation of A455E by Δ27-264 CFTR", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-08 03:15:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/1341485"], "description"=>"<p>Cos7 cells were transfected with both Δ27-264 CFTR and an A455E CFTR construct bearing a GFP tag. Anti-GFP antibodies were used to pull down A455E, and the gels were blotted with anti-CFTR antibody. (n = 3).</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "genetics", "Human genetics", "Autosomal recessive", "cystic fibrosis", "population genetics", "mutation", "Genetic mutation", "Genetics of disease", "Population biology", "Anatomy and physiology", "Clinical genetics", "Pulmonology", "binds"], "article_id"=>896454, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Liudmila Cebotaru", "Daniele Rapino", "Valeriu Cebotaru", "William B. Guggino"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0085183.g006", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_A455E_binds_to_916_27_264_CFTR_/896454", "title"=>"A455E binds to Δ27-264 CFTR.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-08 03:15:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/1341487"], "description"=>"<p>Cos7 cells were transfected with A455E and treated with the correctors C3 or C4 for 16 h at the specified concentrations. Note that C4 had a profound effect on the immature B band of A455E as well as causing an increase in the mature C band (<b>A, B</b>). C3 did not have a significant effect (<b>C, D</b>). (n = 3).</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "genetics", "Human genetics", "Autosomal recessive", "cystic fibrosis", "population genetics", "mutation", "Genetic mutation", "Genetics of disease", "Population biology", "Anatomy and physiology", "Clinical genetics", "Pulmonology"], "article_id"=>896456, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Liudmila Cebotaru", "Daniele Rapino", "Valeriu Cebotaru", "William B. Guggino"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0085183.g007", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Correctors_can_rescue_A455E_/896456", "title"=>"Correctors can rescue A455E.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-08 03:15:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/1341461"], "description"=>"<p>Cos7 cells were transfected with 2 µg of wild-type, A455E, or ΔF508 CFTR constructs. After 48 h, the cells were lysed, and the total lysate was analyzed by western blotting with anti-human CFTR antibodies. Note that there is much less mature C band in the A455E sample than in the wild-type sample. In this and subsequent blots, some mature C band was detected with A455E (n = 8).</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "genetics", "Human genetics", "Autosomal recessive", "cystic fibrosis", "population genetics", "mutation", "Genetic mutation", "Genetics of disease", "Population biology", "Anatomy and physiology", "Clinical genetics", "Pulmonology", "reduced", "mature"], "article_id"=>896446, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Liudmila Cebotaru", "Daniele Rapino", "Valeriu Cebotaru", "William B. Guggino"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0085183.g001", "stats"=>{"downloads"=>1, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_A455E_has_reduced_expression_of_mature_CFTR_/896446", "title"=>"A455E has reduced expression of mature CFTR.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-08 03:15:28"}
  • {"files"=>["https://ndownloader.figshare.com/files/1341462"], "description"=>"<p>Cells transfected with A455E cDNA were treated either with MG132, a more general inhibitor (n = 6) (<b>A, B</b>), or the more specific inhibitor of proteasomes, PS341 (n = 2) (<b>C</b>). Note that in both cases, proteasome inhibition caused an increase in both the immature B and mature C bands of A455E.</p>", "links"=>[], "tags"=>["Biochemistry", "proteins", "genetics", "Human genetics", "Autosomal recessive", "cystic fibrosis", "population genetics", "mutation", "Genetic mutation", "Genetics of disease", "Population biology", "Anatomy and physiology", "Clinical genetics", "Pulmonology"], "article_id"=>896447, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Liudmila Cebotaru", "Daniele Rapino", "Valeriu Cebotaru", "William B. Guggino"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0085183.g002", "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Proteasome_inhibition_/896447", "title"=>"Proteasome inhibition.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-08 03:15:28"}

PMC Usage Stats | Further Information

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  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
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  • {"unique-ip"=>"23", "full-text"=>"27", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
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  • {"unique-ip"=>"20", "full-text"=>"20", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"20", "full-text"=>"20", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"13", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"20", "full-text"=>"20", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"26", "full-text"=>"23", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"29", "full-text"=>"38", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
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  • {"unique-ip"=>"28", "full-text"=>"33", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"29", "full-text"=>"31", "pdf"=>"13", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"16", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"17", "full-text"=>"16", "pdf"=>"5", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"17", "full-text"=>"23", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"50", "full-text"=>"60", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"34", "full-text"=>"34", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"19", "full-text"=>"18", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"27", "full-text"=>"29", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"20", "full-text"=>"19", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"24", "full-text"=>"23", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"17", "full-text"=>"18", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"15", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"21", "full-text"=>"20", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"26", "full-text"=>"24", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"21", "full-text"=>"23", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"26", "full-text"=>"28", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"19", "full-text"=>"19", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"32", "full-text"=>"30", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"28", "full-text"=>"34", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"22", "full-text"=>"27", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"19", "full-text"=>"18", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"18", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"24", "full-text"=>"23", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"41", "full-text"=>"46", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"25", "full-text"=>"30", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"19", "full-text"=>"21", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"38", "full-text"=>"63", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"40", "full-text"=>"44", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"56", "full-text"=>"70", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"29", "full-text"=>"32", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"42", "full-text"=>"47", "pdf"=>"10", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"36", "full-text"=>"51", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"20", "full-text"=>"24", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"44", "full-text"=>"49", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"24", "full-text"=>"28", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"40", "full-text"=>"56", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"37", "full-text"=>"42", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"72", "full-text"=>"85", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"31", "full-text"=>"33", "pdf"=>"5", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"70", "full-text"=>"72", "pdf"=>"14", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"37", "full-text"=>"41", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"34", "full-text"=>"39", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"63", "full-text"=>"72", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

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