Cell Surface-Associated Anti-MUC1-Derived Signal Peptide Antibodies: Implications for Cancer Diagnostics and Therapy
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Cell surface-associated anti-MUC1-derived signal peptide antibodies: Implications for cancer diagnostics and therapy", "type"=>"journal", "authors"=>[{"first_name"=>"Riva", "last_name"=>"Kovjazin", "scopus_author_id"=>"6507420472"}, {"first_name"=>"Galit", "last_name"=>"Horn", "scopus_author_id"=>"35200661800"}, {"first_name"=>"Nechama I.", "last_name"=>"Smorodinsky", "scopus_author_id"=>"7004270915"}, {"first_name"=>"Michael Y.", "last_name"=>"Shapira", "scopus_author_id"=>"35498978300"}, {"first_name"=>"Lior", "last_name"=>"Carmon", "scopus_author_id"=>"7801512433"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84896986239", "pmid"=>"24416403", "sgr"=>"84896986239", "doi"=>"10.1371/journal.pone.0085400", "issn"=>"19326203", "pui"=>"372704139"}, "id"=>"79b90139-3b8a-3477-93b8-6fd3ac3bd044", "abstract"=>"The MUC1 tumor associated antigen is highly expressed on a range of tumors. Its broad distribution on primary tumors and metastases renders it an attractive target for immunotherapy. After synthesis MUC1 is cleaved, yielding a large soluble extracellular alpha subunit containing the tandem repeats array (TRA) domain specifically bound, via non-covalent interaction, to a smaller beta subunit containing the transmembrane and cytoplasmic domains. Thus far, inconclusive efficacy has been reported for anti-MUC1 antibodies directed against the soluble alpha subunit. Targeting the cell bound beta subunit, may bypass limitations posed by circulating TRA domains. MUC1's signal peptide (SP) domain promiscuously binds multiple MHC class II and Class I alleles, which upon vaccination, generated robust T-cell immunity against MUC1-positive tumors. This is a first demonstration of non-MHC associated, MUC1 specific, cell surfaces presence for MUC1 SP domain. Polyclonal and monoclonal antibodies generated against MUC1 SP domain specifically bind a large variety of MUC1-positive human solid and haematological tumor cell lines; MUC1-positive bone marrow derived plasma cells obtained from multiple myeloma (MM)-patients, but not MUC1 negative tumors cells, and normal naive primary blood and epithelial cells. Membranal MUC1 SP appears mainly as an independent entity but also co-localized with the full MUC1 molecule. MUC1-SP specific binding in BM-derived plasma cells can assist in selecting patients to be treated with anti-MUC1 SP therapeutic vaccine, ImMucin. A therapeutic potential of the anti-MUC1 SP antibodies was suggested by their ability to support of complement-mediated lysis of MUC1-positive tumor cells but not MUC1 negative tumor cells and normal naive primary epithelial cells. These findings suggest a novel cell surface presence of MUC1 SP domain, a potential therapeutic benefit for anti-MUC1 SP antibodies in MUC1-positive tumors and a selection tool for MM patients to be treated with the anti-MUC1 SP vaccine, ImMucin.", "link"=>"http://www.mendeley.com/research/cell-surfaceassociated-antimuc1derived-signal-peptide-antibodies-implications-cancer-diagnostics-the", "reader_count"=>18, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>4, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>1, "Student > Master"=>2, "Student > Bachelor"=>4}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>4, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>1, "Student > Master"=>2, "Student > Bachelor"=>4}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>10, "Medicine and Dentistry"=>3, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Chemistry"=>{"Chemistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>10}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}}, "reader_count_by_country"=>{"United States"=>1, "South Africa"=>1}, "group_count"=>1}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84896986239"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84896986239?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84896986239&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84896986239&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84896986239", "dc:identifier"=>"SCOPUS_ID:84896986239", "eid"=>"2-s2.0-84896986239", "dc:title"=>"Cell surface-associated anti-MUC1-derived signal peptide antibodies: Implications for cancer diagnostics and therapy", "dc:creator"=>"Kovjazin R.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"9", "prism:issueIdentifier"=>"1", "prism:pageRange"=>nil, "prism:coverDate"=>"2014-01-08", "prism:coverDisplayDate"=>"8 January 2014", "prism:doi"=>"10.1371/journal.pone.0085400", "citedby-count"=>"14", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Vaxil BioTherapeutics Ltd.", "affiliation-city"=>"Nes Ziona", "affiliation-country"=>"Israel"}], "pubmed-id"=>"24416403", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e85400", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0085400", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"71", "xml_views"=>"9", "html_views"=>"405"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"45", "xml_views"=>"0", "html_views"=>"202"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"25", "xml_views"=>"1", "html_views"=>"171"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"34", "xml_views"=>"4", "html_views"=>"165"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"32", "xml_views"=>"1", "html_views"=>"216"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"136"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"27", "xml_views"=>"0", "html_views"=>"183"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"2", "html_views"=>"102"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"22", "xml_views"=>"1", "html_views"=>"113"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"18", "xml_views"=>"2", "html_views"=>"171"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"158"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"2", "html_views"=>"104"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"85"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"62"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"90"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"50"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"57"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"49"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"37", "xml_views"=>"0", "html_views"=>"77"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"18", "xml_views"=>"3", "html_views"=>"72"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"56"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"31"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"33", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"22", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"35"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"70"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"53"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"38"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"45"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"30"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"32"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"38"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"39"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"61"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"28"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"8"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"3", "html_views"=>"14"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"7"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"4"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1341538"], "description"=>"<p><sup>1</sup>% of positive staining was applies in cell which undergo double staining.</p>", "links"=>[], "tags"=>["Clinical immunology", "Immune system", "bone marrow", "Complement system", "immunity", "Humoral immunity", "immunotherapy", "Immunologic techniques", "immunofluorescence", "immunoglobulins", "Major histocompatibility complex", "Diagnostic medicine", "hematology", "Plasma cell disorders", "Multiple myeloma", "Hematologic cancers and related disorders", "oncology", "Cancer treatment", "Antibody therapy", "Cancer detection and diagnosis", "Cancers and neoplasms", "muc1", "sp", "cell-lines"], "article_id"=>896506, "categories"=>["Medicine"], "users"=>["Riva Kovjazin", "Galit Horn", "Nechama I. Smorodinsky", "Michael Y. Shapira", "Lior Carmon"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0085400.t002", "stats"=>{"downloads"=>0, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_cellular_expression_of_MUC1_SP_domain_on_MUC1_positive_tumor_cell_lines_and_primary_na_ve_cells_/896506", "title"=>"<i>The cellular</i> expression of MUC1 SP domain on MUC1 positive tumor cell-lines and primary naïve cells.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-01-08 03:19:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/1341533"], "description"=>"<p>The cell surface presence of the MUC1 SP domain was evaluated by gated FACS analysis on PC cells in fresh BM aspirates obtained from MM patients (A–E) and normal naïve sample (F–H). Polyclonal anti-MUC1 SP (R23IgG) was used to determine MUC1 SP expression; MUC1 TRA domain expression was determined with H23 mAb. For each experiment, species-matched control antibodies for MUC1 staining were used: either normal mouse IgG–FITC (B), or normal rabbit IgG-FITC (D). Anti-MUC1 SP mAb (SPmAb-2.1) was used to determined MUC1 SP expression; MUC1 TRA domain expression was determined with H23 mAb.</p>", "links"=>[], "tags"=>["Clinical immunology", "Immune system", "bone marrow", "Complement system", "immunity", "Humoral immunity", "immunotherapy", "Immunologic techniques", "immunofluorescence", "immunoglobulins", "Major histocompatibility complex", "Diagnostic medicine", "hematology", "Plasma cell disorders", "Multiple myeloma", "Hematologic cancers and related disorders", "oncology", "Cancer treatment", "Antibody therapy", "Cancer detection and diagnosis", "Cancers and neoplasms", "sp", "antibodies", "muc1-expressing", "mm", "cells", "bm"], "article_id"=>896501, "categories"=>["Medicine"], "users"=>["Riva Kovjazin", "Galit Horn", "Nechama I. Smorodinsky", "Michael Y. Shapira", "Lior Carmon"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0085400.g003", "stats"=>{"downloads"=>0, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Anti_MUC1_SP_antibodies_bind_MUC1_expressing_MM_cells_in_fresh_human_BM_aspirates_/896501", "title"=>"Anti-MUC1 SP antibodies bind MUC1-expressing MM cells in fresh human BM aspirates.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-08 03:19:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/1341529"], "description"=>"<p>The cell surface presence and similarity analysis of the MUC1 SP domain and MUC1 TRA was evaluated on OVACAR-3 MUC1-positive and ES-2 MUC1-negative tumor cell lines by imagestream analysis using PE- and APC-tagged anti-MUC1 SP mAbs SPmAb-2.1, SPmAb-6, and anti-MUC1 TRA H23 mAb (A). Fluorescence microscopy analysis was also performed on ES-2 MUC1-transfected ovarian cells vs. the parent MUC1-negative cells (B). BF stand for bright field and secondary stand for anti-mouse IgG antibodies.</p>", "links"=>[], "tags"=>["Clinical immunology", "Immune system", "bone marrow", "Complement system", "immunity", "Humoral immunity", "immunotherapy", "Immunologic techniques", "immunofluorescence", "immunoglobulins", "Major histocompatibility complex", "Diagnostic medicine", "hematology", "Plasma cell disorders", "Multiple myeloma", "Hematologic cancers and related disorders", "oncology", "Cancer treatment", "Antibody therapy", "Cancer detection and diagnosis", "Cancers and neoplasms", "muc1", "sp", "membrane", "muc1-positive"], "article_id"=>896497, "categories"=>["Medicine"], "users"=>["Riva Kovjazin", "Galit Horn", "Nechama I. Smorodinsky", "Michael Y. Shapira", "Lior Carmon"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0085400.g002", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Detection_of_MUC1_SP_on_the_membrane_of_MUC1_positive_tumor_cells_/896497", "title"=>"Detection of MUC1 SP on the membrane of MUC1-positive tumor cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-08 03:19:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/1341527"], "description"=>"<p>The specificity and defined minimal epitope of the generated anti-MUC1 SP polyclonal antibodies (A–C) and mAbs SPmAb-2.1 and SPmAb-6 (D–E) were evaluated against the immunizing peptide MUC1-SP-M and its internal epitopes MUC1-SP-S1 to S5, in ELISA assays. MUC1's TRA epitope, MUC1-TRA-L, and the non-MUC1 SP domain BAGE-SP-L, were used in these assays as negative controls.</p>", "links"=>[], "tags"=>["Clinical immunology", "Immune system", "bone marrow", "Complement system", "immunity", "Humoral immunity", "immunotherapy", "Immunologic techniques", "immunofluorescence", "immunoglobulins", "Major histocompatibility complex", "Diagnostic medicine", "hematology", "Plasma cell disorders", "Multiple myeloma", "Hematologic cancers and related disorders", "oncology", "Cancer treatment", "Antibody therapy", "Cancer detection and diagnosis", "Cancers and neoplasms", "muc1", "sp-specific"], "article_id"=>896495, "categories"=>["Medicine"], "users"=>["Riva Kovjazin", "Galit Horn", "Nechama I. Smorodinsky", "Michael Y. Shapira", "Lior Carmon"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0085400.g001", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Generation_of_MUC1_SP_specific_antibodies_/896495", "title"=>"Generation of MUC1 SP-specific antibodies.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-08 03:19:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/1341536"], "description"=>"<p><sup>1</sup> Nomenclature used for internal ID is: target antigen (e.g. MUC1), targeted domain (e.g. SP or TRA), and peptide lengths were \"S\" (short), \"M\" (moderate), \"L\" (long)<b>.</b></p>", "links"=>[], "tags"=>["Clinical immunology", "Immune system", "bone marrow", "Complement system", "immunity", "Humoral immunity", "immunotherapy", "Immunologic techniques", "immunofluorescence", "immunoglobulins", "Major histocompatibility complex", "Diagnostic medicine", "hematology", "Plasma cell disorders", "Multiple myeloma", "Hematologic cancers and related disorders", "oncology", "Cancer treatment", "Antibody therapy", "Cancer detection and diagnosis", "Cancers and neoplasms"], "article_id"=>896504, "categories"=>["Medicine"], "users"=>["Riva Kovjazin", "Galit Horn", "Nechama I. Smorodinsky", "Michael Y. Shapira", "Lior Carmon"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0085400.t001", "stats"=>{"downloads"=>3, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Peptides_used_in_this_study_/896504", "title"=>"Peptides used in this study.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-01-08 03:19:45"}
  • {"files"=>["https://ndownloader.figshare.com/files/1341534"], "description"=>"<p>The cytotoxic properties of the anti-MUC1 SP polyclonal R23IgG (A) and monoclonal SPmAb-2.1, SPmAb-6 mAb (B) were evaluated by CDC analysis using various solid, non-solid, MUC1-positive and -negative tumors and HMEC. The MUC1 TRA domain-specific mAb H23, NMS and NRS and no (W/O) antibodies were evaluated as controls.</p>", "links"=>[], "tags"=>["Clinical immunology", "Immune system", "bone marrow", "Complement system", "immunity", "Humoral immunity", "immunotherapy", "Immunologic techniques", "immunofluorescence", "immunoglobulins", "Major histocompatibility complex", "Diagnostic medicine", "hematology", "Plasma cell disorders", "Multiple myeloma", "Hematologic cancers and related disorders", "oncology", "Cancer treatment", "Antibody therapy", "Cancer detection and diagnosis", "Cancers and neoplasms", "specificity", "anti-muc1", "sp", "cdc"], "article_id"=>896502, "categories"=>["Medicine"], "users"=>["Riva Kovjazin", "Galit Horn", "Nechama I. Smorodinsky", "Michael Y. Shapira", "Lior Carmon"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0085400.g004", "stats"=>{"downloads"=>0, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Efficacy_and_specificity_of_anti_MUC1_SP_antibodies_8211_mediated_CDC_mediated_/896502", "title"=>"Efficacy and specificity of anti-MUC1 SP antibodies–mediated CDC mediated.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-08 03:19:45"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"46", "full-text"=>"47", "pdf"=>"28", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"55", "full-text"=>"64", "pdf"=>"29", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"18", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"39", "full-text"=>"42", "pdf"=>"18", "abstract"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"23", "full-text"=>"19", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"28", "full-text"=>"28", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"14", "full-text"=>"17", "pdf"=>"19", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"17", "full-text"=>"15", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"19", "full-text"=>"19", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"19", "full-text"=>"19", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"22", "full-text"=>"32", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"16", "full-text"=>"24", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"18", "full-text"=>"18", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"17", "full-text"=>"21", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"18", "full-text"=>"15", "pdf"=>"9", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"21", "full-text"=>"23", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"17", "full-text"=>"22", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"20", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"24", "full-text"=>"19", "pdf"=>"7", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"14", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"37", "full-text"=>"33", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"20", "full-text"=>"16", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"14", "full-text"=>"14", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"14", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"10", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"16", "full-text"=>"20", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"18", "full-text"=>"19", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"23", "full-text"=>"30", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"16", "full-text"=>"22", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"15", "full-text"=>"16", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"14", "full-text"=>"19", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"16", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"15", "full-text"=>"18", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"14", "full-text"=>"13", "pdf"=>"9", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"14", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"15", "full-text"=>"15", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[291]}, {"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[282]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[286]}, {"subject_area"=>"/Biology and life sciences/Immunology", "average_usage"=>[293]}, {"subject_area"=>"/Biology and life sciences/Physiology", "average_usage"=>[280]}]}
Loading … Spinner
There are currently no alerts