RNA Polymerase III Transcriptomes in Human Embryonic Stem Cells and Induced Pluripotent Stem Cells, and Relationships with Pluripotency Transcription Factors
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"RNA polymerase III transcriptomes in human embryonic stem cells and induced pluripotent stem cells, and relationships with pluripotency transcription factors", "type"=>"journal", "authors"=>[{"first_name"=>"Ravi K.", "last_name"=>"Alla", "scopus_author_id"=>"36105751400"}, {"first_name"=>"Bradley R.", "last_name"=>"Cairns", "scopus_author_id"=>"7004508741"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84924258508", "sgr"=>"84924258508", "pui"=>"372852955", "isbn"=>"1932-6203 (Electronic)\\n1932-6203 (Linking)", "pmid"=>"24465633", "doi"=>"10.1371/journal.pone.0085648"}, "id"=>"65ad5afc-5b1a-344f-ae04-d75d58b27b68", "abstract"=>"Recent genomic approaches have revealed that the repertoire of RNA Pol III-transcribed genes varies in different human cell types, and that this variation is likely determined by a combination of the chromatin landscape, cell-specific DNA-binding transcription factors, and collaboration with RNA Pol II. Although much is known about this regulation in differentiated human cells, there is presently little understanding of this aspect of the Pol III system in human ES cells. Here, we determine the occupancy profiles of Pol III components in human H1 ES cells, and also induced pluripotent cells, and compare to known profiles of chromatin, transcription factors, and RNA expression. We find a relatively large fraction of the Pol III repertoire occupied in human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). In ES cells we find clear correlations between Pol III occupancy and active chromatin. Interestingly, we find a highly significant fraction of Pol III-occupied genes with adjacent binding events by pluripotency factors in ES cells, especially NANOG. Notably, in human ES cells we find H3K27me3 adjacent to but not overlapping many active Pol III loci. We observe in all such cases, a peak of H3K4me3 and/or RNA Pol II, between the H3K27me3 and Pol III binding peaks, suggesting that H3K4me3 and Pol II activity may “insulate” Pol III from neighboring repressive H3K27me3. Further, we find iPSCs have a larger Pol III repertoire than their precursors. Finally, the active Pol III genome in iPSCs is not completely reprogrammed to a hESC like state and partially retains the transcriptional repertoire of the precursor. Together, our correlative results are consistent with Pol III binding and activity in human ES cells being enabled by active/permissive chromatin that is shaped in part by the pluripotency network of transcription factors and RNA Pol II activity.", "link"=>"http://www.mendeley.com/research/rna-polymerase-iii-transcriptomes-human-embryonic-stem-cells-induced-pluripotent-stem-cells-relation-2", "reader_count"=>29, "reader_count_by_academic_status"=>{"Student > Doctoral Student"=>1, "Researcher"=>10, "Student > Ph. D. Student"=>11, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>2, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Student > Doctoral Student"=>1, "Researcher"=>10, "Student > Ph. D. Student"=>11, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>2, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>7, "Agricultural and Biological Sciences"=>18, "Medicine and Dentistry"=>2, "Chemistry"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Chemistry"=>{"Chemistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>18}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>7}}, "reader_count_by_country"=>{"Denmark"=>1, "Australia"=>1, "Germany"=>1}, "group_count"=>3}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84924258508"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84924258508?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84924258508&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84924258508&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84924258508", "dc:identifier"=>"SCOPUS_ID:84924258508", "eid"=>"2-s2.0-84924258508", "dc:title"=>"RNA polymerase III transcriptomes in human embryonic stem cells and induced pluripotent stem cells, and relationships with pluripotency transcription factors", "dc:creator"=>"Alla R.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"9", "prism:issueIdentifier"=>"1", "prism:pageRange"=>nil, "prism:coverDate"=>"2014-01-20", "prism:coverDisplayDate"=>"20 January 2014", "prism:doi"=>"10.1371/journal.pone.0085648", "citedby-count"=>"19", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"University of Utah, School of Medicine", "affiliation-city"=>"Salt Lake City", "affiliation-country"=>"United States"}, {"@_fa"=>"true", "affilname"=>"Howard Hughes Medical Institute", "affiliation-city"=>"Chevy Chase", "affiliation-country"=>"United States"}], "pubmed-id"=>"24465633", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e85648", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0085648", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Twitter

Counter

  • {"month"=>"1", "year"=>"2014", "pdf_views"=>"63", "xml_views"=>"7", "html_views"=>"182"}
  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"35", "xml_views"=>"1", "html_views"=>"163"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"23", "xml_views"=>"2", "html_views"=>"196"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"32", "xml_views"=>"3", "html_views"=>"152"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"23", "xml_views"=>"0", "html_views"=>"167"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"141"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"19", "xml_views"=>"0", "html_views"=>"127"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"21", "xml_views"=>"2", "html_views"=>"112"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"19", "xml_views"=>"1", "html_views"=>"149"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"19", "xml_views"=>"1", "html_views"=>"174"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"164"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"2", "html_views"=>"61"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"18", "xml_views"=>"0", "html_views"=>"63"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"1", "html_views"=>"46"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"88"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"42"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"150"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"32"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"34"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"35"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"36"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"28"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"33"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"32"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"64"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"3", "html_views"=>"14"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"6"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1351491"], "description"=>"<p>IE – Internal Element, DSE – Distal Sequence Element, PSE – Proximal Sequence Element. Adapted from Ref 5.</p>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "Gene regulation", "developmental biology", "stem cells", "Embryonic stem cells", "Induced pluripotent stem cells", "genetics", "epigenetics", "genomics", "Genome analysis tools", "transcriptomes", "Molecular cell biology", "Cellular types", "gene expression", "Histone modification", "chromatin", "iii", "classes", "promoter", "transcription"], "article_id"=>904226, "categories"=>["Biological Sciences"], "users"=>["Ravi K. Alla", "Bradley R. Cairns"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0085648.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Pol_III_gene_classes_based_on_promoter_architecture_and_associated_transcription_factors_/904226", "title"=>"Pol III gene classes based on promoter architecture and associated transcription factors.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-20 03:14:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1351492"], "description"=>"<p><b>A.</b> Chromosome 6 tDNA cluster snapshot showing Pol III QValFDR (red), TFIIIC QValFDR (green) and the H1 small RNA read coverage (magenta) tracks. Chromosome coordinates are shown under the plots, tDNAs are depicted as black vertical lines. Annotations above the chromosome coordinate line (x-axis) are on the (+) strand and those below are on the (−) strand. The y-axis values are −10Log(QValue FDR) scores. <b>B.</b> Venn diagram depicting intersection between mappable tDNAs (blue), Annotated Pol III genes bound by Pol III in H1 cells (red) and Annotated Pol III genes bound by TFIIIC in H1 cells (green). Numbers in parentheses indicate the number of genes in each Venn set. <b>C–E.</b> Pol III QValFDR, TFIIIC QValFDR and small RNA read coverage tracks at Pol III example loci. 7SL2 is a Type II Pol III gene (<b>C</b>); RNAseMRP (<b>D</b>) and 7SK (<b>E</b>) are Type III Pol III genes.</p>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "Gene regulation", "developmental biology", "stem cells", "Embryonic stem cells", "Induced pluripotent stem cells", "genetics", "epigenetics", "genomics", "Genome analysis tools", "transcriptomes", "Molecular cell biology", "Cellular types", "gene expression", "Histone modification", "chromatin", "pol", "iii", "tfiiic", "h1"], "article_id"=>904227, "categories"=>["Biological Sciences"], "users"=>["Ravi K. Alla", "Bradley R. Cairns"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0085648.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Occupancy_analysis_of_Pol_III_and_TFIIIC_in_H1_cells_/904227", "title"=>"Occupancy analysis of Pol III and TFIIIC in H1 cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-20 03:14:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1351493"], "description"=>"<p><b>A.</b> Venn diagram showing intersection of Pol III-bound annotated genes in four different cell types, H1 (red), HeLa (cyan), Human Foreskin Fibroblasts - HFF (green) and HEK293T cells (blue). <b>B–D.</b> Pol III QValFDR tracks at example tDNAs that are differentially bound in H1 cells (red) compared to HeLa (cyan) and HEK293 (blue) cells. Arrows mark the genes that differ in Pol III occupancy. Asterisks mark the tDNAs that are similarly occupied in all the cell types. The axes are as described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085648#pone-0085648-g001\" target=\"_blank\">Figure 1</a>. <b>E–G.</b> Pol III QValFDR tracks at example Type II (<b>E</b>) and Type III (<b>F,</b>G) loci that are similarly bound in the three cell types. Asterisks mark the genes that are bound by Pol III in all cell types.</p>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "Gene regulation", "developmental biology", "stem cells", "Embryonic stem cells", "Induced pluripotent stem cells", "genetics", "epigenetics", "genomics", "Genome analysis tools", "transcriptomes", "Molecular cell biology", "Cellular types", "gene expression", "Histone modification", "chromatin", "occupancy", "pol", "iii"], "article_id"=>904228, "categories"=>["Biological Sciences"], "users"=>["Ravi K. Alla", "Bradley R. Cairns"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0085648.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Differential_occupancy_by_Pol_III_in_various_cell_types_/904228", "title"=>"Differential occupancy by Pol III in various cell types.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-20 03:14:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1351494"], "description"=>"<p>Class average maps of histone marks and transcription factors at a 4-axis shows the distance from the TSS of the Pol III gene (0 is the start of the TSS). <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085648#pone-0085648-g004\" target=\"_blank\"><b>Figure 4A</b></a> shows the schematic of a tDNA along the x-axis. The y-axis shows normalized read counts. Note that the scale of the y-axis is different for the various factors and marks. tDNAs were binned based on levels of Pol III binding, top 50 tDNAs (blue), middle 50 tDNAs (red), bottom 50 tDNAs (yellow) and tDNAs not bound by Pol III or TFIIIC (green). Also shown are the intersection fractions of Pol III-bound regions with the mark/factor bound regions (top right of each graph). Intersections are significant with a P-value <0.001.</p>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "Gene regulation", "developmental biology", "stem cells", "Embryonic stem cells", "Induced pluripotent stem cells", "genetics", "epigenetics", "genomics", "Genome analysis tools", "transcriptomes", "Molecular cell biology", "Cellular types", "gene expression", "Histone modification", "chromatin", "marks", "pol", "iii-bound", "genes", "h1"], "article_id"=>904229, "categories"=>["Biological Sciences"], "users"=>["Ravi K. Alla", "Bradley R. Cairns"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0085648.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Chromatin_marks_and_factors_associated_with_Pol_III_bound_genes_in_H1_cells_/904229", "title"=>"Chromatin marks and factors associated with Pol III-bound genes in H1 cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-20 03:14:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1351499"], "description"=>"<p><b>A–D</b>. Examples are shown of Pol III-bound genes that intersect with H3K27me3 (within 2 kb). Shown are QValFDR tracks for Pol III (red), TFIIIC (dark green), TBP (cyan), Pol II (teal), H3K4me3 (light green), H2AZ (purple), H3K27me3 (blue) and the H1 small RNA read coverage track (magenta). Axes are as described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085648#pone-0085648-g001\" target=\"_blank\">Figure 1</a>. <b>E.</b> Box plot showing the difference in H3K27me3 enrichment levels between H1 and HeLa cells at Pol III-bound and unbound tDNAs (4 kb flanking TSS-left half of the plot and 500 bp flanking TSS-right half of the plot). Y-axis is the log of the H3K27me3 mark enrichment. Asterisks denote significant P-Value <0.0001. ns denotes a P-Value that is not significant. <b>F.</b> Class average map of H3K4me3 (green), H3K27me3 (blue), Pol II (teal) and Pol III (red) across an average Pol III-bound gene that is marked with H3K27me3. Axes are as described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085648#pone-0085648-g003\" target=\"_blank\">Figure 3</a>.</p>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "Gene regulation", "developmental biology", "stem cells", "Embryonic stem cells", "Induced pluripotent stem cells", "genetics", "epigenetics", "genomics", "Genome analysis tools", "transcriptomes", "Molecular cell biology", "Cellular types", "gene expression", "Histone modification", "chromatin", "pol", "iii-bound", "genes"], "article_id"=>904231, "categories"=>["Biological Sciences"], "users"=>["Ravi K. Alla", "Bradley R. Cairns"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0085648.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Analysis_of_Pol_III_bound_genes_with_regional_H3K27me3_/904231", "title"=>"Analysis of Pol III-bound genes with regional H3K27me3.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-20 03:14:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1351501"], "description"=>"<p><b>A.</b> Venn diagram showing intersection of Pol III-bound regions with Nanog and Oct4 bound regions in H1 cells. Numbers in parentheses indicate total number of regions bound by the factor. <b>B–D.</b> Class average maps of NANOG, OCT4 and SOX2 at a tDNA. Axes are as described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085648#pone-0085648-g003\" target=\"_blank\">Figure 3</a>. Also shown are the intersection fractions of Pol III-bound regions with the mark/factor bound regions (top right of each graph). Intersections are significant with a P-value <0.001. <b>E.</b> Examples of tDNAs that are bound by Pol III and Nanog. Shown are QValFDRs along the y-axis for Pol III (red), TFIIIC (dark green), TBP (cyan), Pol II (teal), H3K4me3 (light green), H2AZ (purple), H3K27me3 (blue), Nanog (orange) and the H1 small RNA read coverage (magenta) track. Axes are as described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085648#pone-0085648-g001\" target=\"_blank\">Figure 1</a>. <b>F–H.</b> Examples of Type II (7SL2) and Type III (RNaseMRP, 7SK) Pol III genes that are bound Pol III and Nanog.</p>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "Gene regulation", "developmental biology", "stem cells", "Embryonic stem cells", "Induced pluripotent stem cells", "genetics", "epigenetics", "genomics", "Genome analysis tools", "transcriptomes", "Molecular cell biology", "Cellular types", "gene expression", "Histone modification", "chromatin", "pluripotency", "pol", "iii-bound", "genes", "h1"], "article_id"=>904233, "categories"=>["Biological Sciences"], "users"=>["Ravi K. Alla", "Bradley R. Cairns"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0085648.g006"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Occupancy_analysis_of_pluripotency_factors_at_Pol_III_bound_genes_in_H1_cells_/904233", "title"=>"Occupancy analysis of pluripotency factors at Pol III-bound genes in H1 cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-20 03:14:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1351502"], "description"=>"<p>Class average maps of transcription factors at a 4-bound gene (red) or an average tDNA not bound by Pol III (blue) in mouse ES cells. Axes are as described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085648#pone-0085648-g003\" target=\"_blank\">Figure 3</a>. Also shown are the intersection fractions of Pol III-bound regions with the mark/factor bound regions (top right of each graph). Intersections are significant with a P-value <0.001.</p>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "Gene regulation", "developmental biology", "stem cells", "Embryonic stem cells", "Induced pluripotent stem cells", "genetics", "epigenetics", "genomics", "Genome analysis tools", "transcriptomes", "Molecular cell biology", "Cellular types", "gene expression", "Histone modification", "chromatin", "pol", "iii-bound", "genes"], "article_id"=>904234, "categories"=>["Biological Sciences"], "users"=>["Ravi K. Alla", "Bradley R. Cairns"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0085648.g007"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Pluripotency_factors_associated_with_Pol_III_bound_genes_in_mESCs_/904234", "title"=>"Pluripotency factors associated with Pol III-bound genes in mESCs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-20 03:14:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1351503"], "description"=>"<p><b>A.</b> Venn diagram showing intersection of Pol III-bound genes in H1, ADS and ADSiPS cells. Numbers in parentheses indicate number of Pol III-bound genes in each of the cell types. Asterisk indicates genes that are commonly bound in ADS and ADS-iPS cells but not in H1 cells. <b>B–E.</b> Examples of Novel Pol III-bound regions in H1 ES cells. Shown are QValFDR values along the y-axis, for Pol III (red), TFIIIC (dark green), TBP (cyan), Pol II (teal), H3K4me3 (light green), H2AZ (purple), H3K27me3 (blue), Nanog (orange) and H1 along with H9 small RNA read coverage (magenta).</p>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "Gene regulation", "developmental biology", "stem cells", "Embryonic stem cells", "Induced pluripotent stem cells", "genetics", "epigenetics", "genomics", "Genome analysis tools", "transcriptomes", "Molecular cell biology", "Cellular types", "gene expression", "Histone modification", "chromatin", "iii", "occupancy", "precursor", "ips", "cells", "pol", "iii-bound", "regions", "h1"], "article_id"=>904235, "categories"=>["Biological Sciences"], "users"=>["Ravi K. Alla", "Bradley R. Cairns"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0085648.g008"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Pol_III_occupancy_in_precursor_and_iPS_cells_and_novel_Pol_III_bound_regions_in_H1_cells_/904235", "title"=>"Pol III occupancy in precursor and iPS cells and novel Pol III-bound regions in H1 cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-20 03:14:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1351505"], "description"=>"<p>Class average maps of histone marks and transcription factors at a 4-bound gene without Pol III (blue) or a TFIIIC-bound gene with Pol III (red). Axes are as described in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085648#pone-0085648-g003\" target=\"_blank\">Figure 3</a>. Also shown are the intersection fractions of TFIIIC III bound regions with the mark/factor bound regions (top right of each graph). Intersections are significant with a P-value <0.001. The numbers in the parentheses next to the figure labels represent the number of regions interrogated.</p>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "Gene regulation", "developmental biology", "stem cells", "Embryonic stem cells", "Induced pluripotent stem cells", "genetics", "epigenetics", "genomics", "Genome analysis tools", "transcriptomes", "Molecular cell biology", "Cellular types", "gene expression", "Histone modification", "chromatin", "marks", "tfiiic-bound", "regions", "h1"], "article_id"=>904237, "categories"=>["Biological Sciences"], "users"=>["Ravi K. Alla", "Bradley R. Cairns"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0085648.g009"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Chromatin_marks_and_factors_associated_with_TFIIIC_bound_regions_in_H1_cells_/904237", "title"=>"Chromatin marks and factors associated with TFIIIC-bound regions in H1 cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-20 03:14:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1351507"], "description"=>"<p>A - Detected only in Pol III ChIP-chip experiments.</p><p>B - Detected by ChIP-qPCR (<a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085648#pone.0085648.s002\" target=\"_blank\">Figure S2</a>).</p>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "Gene regulation", "developmental biology", "stem cells", "Embryonic stem cells", "Induced pluripotent stem cells", "genetics", "epigenetics", "genomics", "Genome analysis tools", "transcriptomes", "Molecular cell biology", "Cellular types", "gene expression", "Histone modification", "chromatin", "pol", "iii", "genes", "hg18", "genome", "enrichment", "ads", "hela"], "article_id"=>904239, "categories"=>["Biological Sciences"], "users"=>["Ravi K. Alla", "Bradley R. Cairns"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0085648.t001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Annotated_Pol_III_genes_in_the_hg18_human_genome_and_Pol_III_enrichment_in_H1_ADS_iPS_ADS_and_HeLa_cells_/904239", "title"=>"Annotated Pol III genes in the hg18 human genome and Pol III enrichment in H1, ADS-iPS, ADS and HeLa cells.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-01-20 03:14:19"}
  • {"files"=>["https://ndownloader.figshare.com/files/1351509", "https://ndownloader.figshare.com/files/1351510", "https://ndownloader.figshare.com/files/1351511", "https://ndownloader.figshare.com/files/1351512"], "description"=>"<div><p>Recent genomic approaches have revealed that the repertoire of RNA Pol III-transcribed genes varies in different human cell types, and that this variation is likely determined by a combination of the chromatin landscape, cell-specific DNA-binding transcription factors, and collaboration with RNA Pol II. Although much is known about this regulation in differentiated human cells, there is presently little understanding of this aspect of the Pol III system in human ES cells. Here, we determine the occupancy profiles of Pol III components in human H1 ES cells, and also induced pluripotent cells, and compare to known profiles of chromatin, transcription factors, and RNA expression. We find a relatively large fraction of the Pol III repertoire occupied in human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). In ES cells we find clear correlations between Pol III occupancy and active chromatin. Interestingly, we find a highly significant fraction of Pol III-occupied genes with adjacent binding events by pluripotency factors in ES cells, especially NANOG. Notably, in human ES cells we find H3K27me3 adjacent to but not overlapping many active Pol III loci. We observe in all such cases, a peak of H3K4me3 and/or RNA Pol II, between the H3K27me3 and Pol III binding peaks, suggesting that H3K4me3 and Pol II activity may “insulate” Pol III from neighboring repressive H3K27me3. Further, we find iPSCs have a larger Pol III repertoire than their precursors. Finally, the active Pol III genome in iPSCs is not completely reprogrammed to a hESC like state and partially retains the transcriptional repertoire of the precursor. Together, our correlative results are consistent with Pol III binding and activity in human ES cells being enabled by active/permissive chromatin that is shaped in part by the pluripotency network of transcription factors and RNA Pol II activity.</p></div>", "links"=>[], "tags"=>["Computational biology", "Molecular genetics", "Gene regulation", "developmental biology", "stem cells", "Embryonic stem cells", "Induced pluripotent stem cells", "genetics", "epigenetics", "genomics", "Genome analysis tools", "transcriptomes", "Molecular cell biology", "Cellular types", "gene expression", "Histone modification", "chromatin", "polymerase", "iii", "embryonic", "cells", "induced", "pluripotent", "relationships", "pluripotency", "transcription"], "article_id"=>904241, "categories"=>["Biological Sciences"], "users"=>["Ravi K. Alla", "Bradley R. Cairns"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0085648.s001", "https://dx.doi.org/10.1371/journal.pone.0085648.s002", "https://dx.doi.org/10.1371/journal.pone.0085648.s003", "https://dx.doi.org/10.1371/journal.pone.0085648.s004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_RNA_Polymerase_III_Transcriptomes_in_Human_Embryonic_Stem_Cells_and_Induced_Pluripotent_Stem_Cells_and_Relationships_with_Pluripotency_Transcription_Factors_/904241", "title"=>"RNA Polymerase III Transcriptomes in Human Embryonic Stem Cells and Induced Pluripotent Stem Cells, and Relationships with Pluripotency Transcription Factors", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-01-20 03:14:19"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"1"}
  • {"unique-ip"=>"54", "full-text"=>"43", "pdf"=>"24", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"37", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"23", "full-text"=>"16", "pdf"=>"10", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"16", "full-text"=>"18", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"26", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"20", "full-text"=>"13", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"16", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"13", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"17", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"16", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"12", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"23", "full-text"=>"22", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"24", "full-text"=>"28", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"17", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"22", "full-text"=>"22", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"17", "full-text"=>"20", "pdf"=>"0", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"21", "full-text"=>"21", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"17", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"12", "full-text"=>"11", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"16", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"2"}
  • {"unique-ip"=>"3", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"14", "full-text"=>"15", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"5", "cited-by"=>"1", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"10", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"9", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"9", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[282]}, {"subject_area"=>"/Biology and life sciences/Developmental biology", "average_usage"=>[285]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[306, 482]}]}
Loading … Spinner
There are currently no alerts