A Distinct Type of Heterochromatin at the Telomeric Region of the Drosophila melanogaster Y Chromosome
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{"title"=>"A distinct type of heterochromatin at the telomeric region of the Drosophila melanogaster Y chromosome", "type"=>"journal", "authors"=>[{"first_name"=>"Sidney H.", "last_name"=>"Wang", "scopus_author_id"=>"35081393200"}, {"first_name"=>"Ruth", "last_name"=>"Nan", "scopus_author_id"=>"57196603033"}, {"first_name"=>"Maria C.", "last_name"=>"Accardo", "scopus_author_id"=>"16680633300"}, {"first_name"=>"Monica", "last_name"=>"Sentmanat", "scopus_author_id"=>"55347606400"}, {"first_name"=>"Patrizio", "last_name"=>"Dimitri", "scopus_author_id"=>"55970452400"}, {"first_name"=>"Sarah C.R.", "last_name"=>"Elgin", "scopus_author_id"=>"7005950231"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84899837704", "doi"=>"10.1371/journal.pone.0086451", "pui"=>"373023182", "pmid"=>"24475122", "scopus"=>"2-s2.0-84899837704", "issn"=>"19326203", "isbn"=>"1932-6203"}, "id"=>"5e912d76-7056-3c5b-bfe1-562429f36b73", "abstract"=>"Heterochromatin assembly and its associated phenotype, position effect variegation (PEV), provide an informative system to study chromatin structure and genome packaging. In the fruit fly Drosophila melanogaster, the Y chromosome is entirely heterochromatic in all cell types except the male germline; as such, Y chromosome dosage is a potent modifier of PEV. However, neither Y heterochromatin composition, nor its assembly, has been carefully studied. Here, we report the mapping and characterization of eight reporter lines that show male-specific PEV. In all eight cases, the reporter insertion sites lie in the telomeric transposon array (HeT-A and TART-B2 homologous repeats) of the Y chromosome short arm (Ys). Investigations of the impact on the PEV phenotype of mutations in known heterochromatin proteins (i.e., modifiers of PEV) show that this Ys telomeric region is a unique heterochromatin domain: it displays sensitivity to mutations in HP1a, EGG and SU(VAR)3-9, but no sensitivity to Su(z)2 mutations. It appears that the endo-siRNA pathway plays a major targeting role for this domain. Interestingly, an ectopic copy of 1360 is sufficient to induce a piRNA targeting mechanism to further enhance silencing of a reporter cytologically localized to the Ys telomere. These results demonstrate the diversity of heterochromatin domains, and the corresponding variation in potential targeting mechanisms.", "link"=>"http://www.mendeley.com/research/distinct-type-heterochromatin-telomeric-region-drosophila-melanogaster-y-chromosome", "reader_count"=>20, "reader_count_by_academic_status"=>{"Student > Doctoral Student"=>4, "Researcher"=>3, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>1, "Other"=>2, "Student > Master"=>1, "Professor"=>2}, "reader_count_by_user_role"=>{"Student > Doctoral Student"=>4, "Researcher"=>3, "Student > Ph. D. Student"=>7, "Student > Postgraduate"=>1, "Other"=>2, "Student > Master"=>1, "Professor"=>2}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>9, "Agricultural and Biological Sciences"=>11}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>11}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>9}}, "reader_count_by_country"=>{"United States"=>1, "Japan"=>1}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1360441"], "description"=>"<p>(a) Pigment level quantification representing the level of PEV. Progeny from a cross with <i>yw<sup>67c23</sup></i> is used as the wild type control. The allele used in each cross is shown on the X-axis. (Bars represent the average pigment level ± standard error. Asterisks are used to indicate mutant alleles that show statistically significant modifier activities, single, p<0.05; double, p<0.005.) (b) Representative pictures showing the dominant impact of the mutations on the fly eyes. The allele used for each modifier is listed below each column. The reporter line used is shown to the left of each row.</p>", "links"=>[], "tags"=>["genetics", "epigenetics", "chromatin", "RNA interference", "gene expression", "Molecular genetics", "Gene regulation", "genomics", "Chromosome biology", "Chromosome structure and function", "Model organisms", "Animal models", "Drosophila melanogaster", "Molecular cell biology", "y-linked", "pev", "lines", "mutations", "modifiers", "tpe"], "article_id"=>913127, "categories"=>["Biological Sciences"], "users"=>["Sidney H. Wang", "Ruth Nan", "Maria C. Accardo", "Monica Sentmanat", "Patrizio Dimitri", "Sarah C. R. Elgin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0086451.g002", "stats"=>{"downloads"=>0, "page_views"=>29, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Response_of_Y_linked_PEV_reporter_lines_to_mutations_in_well_known_modifiers_of_PEV_and_TPE_dominant_effects_/913127", "title"=>"Response of Y-linked PEV reporter lines to mutations in well-known modifiers of PEV and TPE (dominant effects).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-24 03:18:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/1360451"], "description"=>"<p>(a) Pigment level quantification indicating the extent of the suppression of PEV. (Bars represent the average pigment level ± standard error. Asterisks are used to indicate mutant alleles that show statistical significant modifier activities, single, p<0.05; double, p<0.005.) Pathways requiring the genes tested are indicated below the allele names. (b) Representative pictures showing the dominant impacts on PEV in the fly eye from mutations disrupting small RNA pathways.</p>", "links"=>[], "tags"=>["genetics", "epigenetics", "chromatin", "RNA interference", "gene expression", "Molecular genetics", "Gene regulation", "genomics", "Chromosome biology", "Chromosome structure and function", "Model organisms", "Animal models", "Drosophila melanogaster", "Molecular cell biology", "mutations", "components", "rna", "pathways", "variegation", "y-linked"], "article_id"=>913137, "categories"=>["Biological Sciences"], "users"=>["Sidney H. Wang", "Ruth Nan", "Maria C. Accardo", "Monica Sentmanat", "Patrizio Dimitri", "Sarah C. R. Elgin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0086451.g004", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Impacts_of_mutations_in_components_of_the_small_RNA_pathways_on_the_level_of_variegation_of_the_Y_linked_reporters_/913137", "title"=>"Impacts of mutations in components of the small RNA pathways on the level of variegation of the Y-linked reporters.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-24 03:18:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/1360448"], "description"=>"<p>(a) Pigment level quantification showing the level of expression. (Bars represent the average pigment level ± standard error. Asterisks are used to indicate mutant alleles that show statistical significant modifier activities, single, p<0.05; double, p<0.005.) 1X 4<sup>th</sup> and 3X 4<sup>th</sup> represent the copy number of the 4<sup>th</sup> chromosome in the assayed flies. Note that the control line <i>yw<sup>67C23</sup></i> has 2X 4<sup>th</sup> (See <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086451#pone.0086451.s001\" target=\"_blank\">Table S1</a> for information on the fly lines used.), the t test on 1X 4<sup>th</sup> examines E(var) activities of the allele (see materials and methods for details). (b) Representative pictures showing the dominant impacts on PEV in the fly eye from mutations disrupting HMT activities.</p>", "links"=>[], "tags"=>["genetics", "epigenetics", "chromatin", "RNA interference", "gene expression", "Molecular genetics", "Gene regulation", "genomics", "Chromosome biology", "Chromosome structure and function", "Model organisms", "Animal models", "Drosophila melanogaster", "Molecular cell biology", "mutations", "hmts", "dosage", "chromosome", "variegation", "y-linked"], "article_id"=>913134, "categories"=>["Biological Sciences"], "users"=>["Sidney H. Wang", "Ruth Nan", "Maria C. Accardo", "Monica Sentmanat", "Patrizio Dimitri", "Sarah C. R. Elgin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0086451.g003", "stats"=>{"downloads"=>1, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Impact_of_mutations_in_HMTs_and_of_dosage_of_the_4_th_chromosome_on_the_level_of_variegation_of_the_Y_linked_reporters_/913134", "title"=>"Impact of mutations in HMTs and of dosage of the 4<sup>th</sup> chromosome on the level of variegation of the Y-linked reporters.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-24 03:18:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/1360438"], "description"=>"<p>(a) Alignment of reporter insertion flanking sequences to a consensus sequence of telomeric retro-transposon <i>TART-B2</i>. The 1 Kb region in the 3′-UTR harboring the insertion sites is magnified. The effective sequence read length for each reporter line is represented by the red line aligned to the region. The red dot at the end of each red line indicates the 5′ end of the reporter. The reporter flanking sequence of line 5m298 starts in the 3′ end of a <i>TART-B2</i> element and extends upstream to a neighboring <i>HeT-A</i> element, suggesting insertion into a partial fragment of <i>TART</i>. (b) <i>In situ</i> hybridization images of the metaphase chromosomes from third instar larval brain squashes. Only the Y chromosome of the representative metaphase spread is shown. DAPI staining is pseudo-colored in blue and the hybridization signal is in red. (C: centromere, Y<sup>L</sup>: long arm, Y<sup>S</sup>: short arm).</p>", "links"=>[], "tags"=>["genetics", "epigenetics", "chromatin", "RNA interference", "gene expression", "Molecular genetics", "Gene regulation", "genomics", "Chromosome biology", "Chromosome structure and function", "Model organisms", "Animal models", "Drosophila melanogaster", "Molecular cell biology", "hybridization", "y-linked", "pev", "reporters", "telomeric", "transposon", "arrays"], "article_id"=>913125, "categories"=>["Biological Sciences"], "users"=>["Sidney H. Wang", "Ruth Nan", "Maria C. Accardo", "Monica Sentmanat", "Patrizio Dimitri", "Sarah C. R. Elgin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0086451.g001", "stats"=>{"downloads"=>1, "page_views"=>39, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Sequencing_and_in_situ_hybridization_mapping_locate_the_Y_linked_PEV_reporters_in_the_telomeric_transposon_arrays_of_Ys_/913125", "title"=>"Sequencing and <i>in situ</i> hybridization mapping locate the Y-linked PEV reporters in the telomeric transposon arrays of Ys.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-24 03:18:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/1360453"], "description"=>"<div><p>Heterochromatin assembly and its associated phenotype, position effect variegation (PEV), provide an informative system to study chromatin structure and genome packaging. In the fruit fly <i>Drosophila melanogaster</i>, the Y chromosome is entirely heterochromatic in all cell types except the male germline; as such, Y chromosome dosage is a potent modifier of PEV. However, neither Y heterochromatin composition, nor its assembly, has been carefully studied. Here, we report the mapping and characterization of eight reporter lines that show male-specific PEV. In all eight cases, the reporter insertion sites lie in the telomeric transposon array (<i>HeT-A</i> and <i>TART-B2</i> homologous repeats) of the Y chromosome short arm (Ys). Investigations of the impact on the PEV phenotype of mutations in known heterochromatin proteins (i.e., modifiers of PEV) show that this Ys telomeric region is a unique heterochromatin domain: it displays sensitivity to mutations in HP1a, EGG and SU(VAR)3-9, but no sensitivity to <i>Su(z)2</i> mutations. It appears that the endo-siRNA pathway plays a major targeting role for this domain. Interestingly, an ectopic copy of <i>1360</i> is sufficient to induce a piRNA targeting mechanism to further enhance silencing of a reporter cytologically localized to the Ys telomere. These results demonstrate the diversity of heterochromatin domains, and the corresponding variation in potential targeting mechanisms.</p></div>", "links"=>[], "tags"=>["genetics", "epigenetics", "chromatin", "RNA interference", "gene expression", "Molecular genetics", "Gene regulation", "genomics", "Chromosome biology", "Chromosome structure and function", "Model organisms", "Animal models", "Drosophila melanogaster", "Molecular cell biology", "heterochromatin", "telomeric", "chromosome"], "article_id"=>913139, "categories"=>["Biological Sciences"], "users"=>["Sidney H. Wang", "Ruth Nan", "Maria C. Accardo", "Monica Sentmanat", "Patrizio Dimitri", "Sarah C. R. Elgin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0086451", "stats"=>{"downloads"=>6, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/A_Distinct_Type_of_Heterochromatin_at_the_Telomeric_Region_of_the_Drosophila_melanogaster_Y_Chromosome/913139", "title"=>"A Distinct Type of Heterochromatin at the Telomeric Region of the <i>Drosophila melanogaster</i> Y Chromosome", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-01-24 03:18:51"}
  • {"files"=>["https://ndownloader.figshare.com/files/1360452"], "description"=>"<p>(a) Diagram showing the construct used in this line. FRT sites (gray triangles) flanking the ectopic copy of the <i>1360</i> element allow a FLPase mediated excision of the element. (b) <i>In situ</i> hybridization image of metaphase chromosomes of line 1250. The DAPI staining is pseudo-colored in blue and the hybridization signal in red. (c) Pigment level quantification comparing the impact on reporter expression of mutations in different small RNA pathway components with and without the ectopic copy of the <i>1360</i> element in the reporter. (Bars represent the average pigment level ± standard error. Asterisks are used to indicate mutant alleles that show statistical significant modifier activities, single, p<0.05; double, p<0.005.) (d) Representative pictures comparing dominant impacts on PEV in the fly eye from mutations disrupting small RNA pathways, with (+) and without (−) the ectopic copy of the<i>1360</i> element in the reporter.</p>", "links"=>[], "tags"=>["genetics", "epigenetics", "chromatin", "RNA interference", "gene expression", "Molecular genetics", "Gene regulation", "genomics", "Chromosome biology", "Chromosome structure and function", "Model organisms", "Animal models", "Drosophila melanogaster", "Molecular cell biology", "ectopic", "ys", "telomeric", "pev", "pirna-dependent"], "article_id"=>913138, "categories"=>["Biological Sciences"], "users"=>["Sidney H. Wang", "Ruth Nan", "Maria C. Accardo", "Monica Sentmanat", "Patrizio Dimitri", "Sarah C. R. Elgin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0086451.g005", "stats"=>{"downloads"=>2, "page_views"=>40, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_An_ectopic_1360_element_enhances_Ys_telomeric_PEV_via_a_piRNA_dependent_mechanism_/913138", "title"=>"An ectopic <i>1360</i> element enhances Ys telomeric PEV via a piRNA-dependent mechanism.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-24 03:18:51"}

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  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[282]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[286]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[306, 482]}]}
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