Juvenile Hormone Biosynthesis Gene Expression in the corpora allata of Honey Bee (Apis mellifera L.) Female Castes
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{"title"=>"Juvenile hormone biosynthesis gene expression in the corpora allata of honey bee (Apis mellifera L.) female castes", "type"=>"journal", "authors"=>[{"first_name"=>"Ana Durvalina", "last_name"=>"Bomtorin", "scopus_author_id"=>"55322433000"}, {"first_name"=>"Aline", "last_name"=>"Mackert", "scopus_author_id"=>"23991034900"}, {"first_name"=>"Gustavo Conrado Couto", "last_name"=>"Rosa", "scopus_author_id"=>"56157183700"}, {"first_name"=>"Livia Maria", "last_name"=>"Moda", "scopus_author_id"=>"6506062622"}, {"first_name"=>"Juliana Ramos", "last_name"=>"Martins", "scopus_author_id"=>"37037961600"}, {"first_name"=>"Márcia Maria Gentile", "last_name"=>"Bitondi", "scopus_author_id"=>"6603798214"}, {"first_name"=>"Klaus", "last_name"=>"Hartfelder", "scopus_author_id"=>"7003269869"}, {"first_name"=>"Zilá Luz Paulino", "last_name"=>"Simões", "scopus_author_id"=>"6603950692"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"24489805", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0086923", "pui"=>"373070624", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "scopus"=>"2-s2.0-84900411056", "sgr"=>"84900411056"}, "id"=>"37282430-16e2-30fb-8a0d-760a9cb9fb5a", "abstract"=>"Juvenile hormone (JH) controls key events in the honey bee life cycle, viz. caste development and age polyethism. We quantified transcript abundance of 24 genes involved in the JH biosynthetic pathway in the corpora allata-corpora cardiaca (CA-CC) complex. The expression of six of these genes showing relatively high transcript abundance was contrasted with CA size, hemolymph JH titer, as well as JH degradation rates and JH esterase (jhe) transcript levels. Gene expression did not match the contrasting JH titers in queen and worker fourth instar larvae, but jhe transcript abundance and JH degradation rates were significantly lower in queen larvae. Consequently, transcriptional control of JHE is of importance in regulating larval JH titers and caste development. In contrast, the same analyses applied to adult worker bees allowed us inferring that the high JH levels in foragers are due to increased JH synthesis. Upon RNAi-mediated silencing of the methyl farnesoate epoxidase gene (mfe) encoding the enzyme that catalyzes methyl farnesoate-to-JH conversion, the JH titer was decreased, thus corroborating that JH titer regulation in adult honey bees depends on this final JH biosynthesis step. The molecular pathway differences underlying JH titer regulation in larval caste development versus adult age polyethism lead us to propose that mfe and jhe genes be assayed when addressing questions on the role(s) of JH in social evolution.", "link"=>"http://www.mendeley.com/research/juvenile-hormone-biosynthesis-gene-expression-corpora-allata-honey-bee-apis-mellifera-l-female-caste-1", "reader_count"=>56, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Professor > Associate Professor"=>3, "Researcher"=>11, "Student > Doctoral Student"=>5, "Student > Ph. D. Student"=>14, "Student > Master"=>11, "Student > Bachelor"=>9, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Professor > Associate Professor"=>3, "Researcher"=>11, "Student > Doctoral Student"=>5, "Student > Ph. D. Student"=>14, "Student > Master"=>11, "Student > Bachelor"=>9, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>4, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>10, "Agricultural and Biological Sciences"=>41}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>41}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>10}, "Unspecified"=>{"Unspecified"=>4}, "Environmental Science"=>{"Environmental Science"=>1}}, "reader_count_by_country"=>{"United States"=>2, "Brazil"=>2, "France"=>2, "Germany"=>1, "Spain"=>1}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1366259"], "description"=>"<p>Enzyme Commission (E.C.) classification and enzyme functions are shown.</p>", "links"=>[], "tags"=>["Biochemistry", "hormones", "developmental biology", "Molecular development", "genetics", "Animal genetics", "gene expression", "Molecular genetics", "Molecular cell biology", "Zoology", "Entomology", "encoding", "enzymes", "jh", "biosynthetic", "pathway", "blastp", "comparisons", "sequences", "genome"], "article_id"=>917901, "categories"=>["Biological Sciences"], "users"=>["Ana Durvalina Bomtorin", "Aline Mackert", "Gustavo Conrado Couto Rosa", "Livia Maria Moda", "Juliana Ramos Martins", "Marcia Maria Gentile Bitondi", "Klaus Hartfelder", "Zilá Luz Paulino Simões"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0086923.t001", "stats"=>{"downloads"=>12, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Genes_encoding_enzymes_of_the_JH_biosynthetic_pathway_in_Apis_mellifera_Prediction_based_on_BLASTP_comparisons_using_Anopheles_gambiae_and_Drosophila_melanogaster_sequences_against_the_honey_bee_genome_version_4_0_/917901", "title"=>"Genes encoding enzymes of the JH biosynthetic pathway in <i>Apis mellifera</i>. Prediction based on BLASTP comparisons using <i>Anopheles gambiae</i> and <i>Drosophila melanogaster</i> sequences against the honey bee genome (version 4.0).", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-01-29 03:42:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/1366255"], "description"=>"<p>Newly-emerged workers were kept for 7 days in cages and fed on a protein-enriched diet (beebread plus syrup), or on a pure carbohydrate diet (syrup only). Each bar represents the mean ± SEM of three independent samples, each consisting of eight CA-CC pairs. (**) t-test; p≤0.001; (*) t-test; p≤0.05.</p>", "links"=>[], "tags"=>["Biochemistry", "hormones", "developmental biology", "Molecular development", "genetics", "Animal genetics", "gene expression", "Molecular genetics", "Molecular cell biology", "Zoology", "Entomology", "transcript", "genes", "encoding", "enzymes", "jh", "biosynthesis", "pathway", "ca-cc"], "article_id"=>917897, "categories"=>["Biological Sciences"], "users"=>["Ana Durvalina Bomtorin", "Aline Mackert", "Gustavo Conrado Couto Rosa", "Livia Maria Moda", "Juliana Ramos Martins", "Marcia Maria Gentile Bitondi", "Klaus Hartfelder", "Zilá Luz Paulino Simões"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0086923.g003", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_diet_on_transcript_levels_of_genes_encoding_enzymes_of_the_JH_biosynthesis_pathway_and_expressed_in_CA_CC_complexes_/917897", "title"=>"Effect of diet on transcript levels of genes encoding enzymes of the JH biosynthesis pathway and expressed in CA-CC complexes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-29 03:42:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/1366253"], "description"=>"<p>Genes are grouped according to the function of the corresponding enzymes: (A) basal mitochondrial metabolism; (B, C) mevalonate pathway; (D) JH-specific steps; (E) methyl moiety transfer. Total RNA extracted from <i>corpora allata</i>-<i>corpora cardiaca</i> complexes (CA-CC), brain, fat body (FB), and ovaries were used for transcript quantification by real time RT-PCR (RT-qPCR). Each column in the graphs represents transcript levels in a single sample of 20–25 pooled CA-CC complexes, and 10 pooled brains, fat bodies and ovaries. The highest expression value for each gene was converted to 1. In the insert (C) the highest expression level among <i>fpps</i> genes was converted to 1.</p>", "links"=>[], "tags"=>["Biochemistry", "hormones", "developmental biology", "Molecular development", "genetics", "Animal genetics", "gene expression", "Molecular genetics", "Molecular cell biology", "Zoology", "Entomology", "jh", "biosynthesis", "pathway", "genes", "tissues", "forager"], "article_id"=>917895, "categories"=>["Biological Sciences"], "users"=>["Ana Durvalina Bomtorin", "Aline Mackert", "Gustavo Conrado Couto Rosa", "Livia Maria Moda", "Juliana Ramos Martins", "Marcia Maria Gentile Bitondi", "Klaus Hartfelder", "Zilá Luz Paulino Simões"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0086923.g001", "stats"=>{"downloads"=>3, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_of_JH_biosynthesis_pathway_genes_in_tissues_from_forager_bees_/917895", "title"=>"Expression of JH biosynthesis pathway genes in tissues from forager bees.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-29 03:42:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/1366254"], "description"=>"<p>(A) relative expression levels of genes encoding enzymes of the JH biosynthesis pathway in CA-CC complexes; transcript levels were determined by RT-qPCR using a pool of 10 CA-CC pairs; <i>rp49</i> expression was used for normalization. The highest expression value for each gene was converted to 1. (B) Hemolymph JH titers measured by RIA. (C and D) CA diameter and diameter of CA nuclei measured by confocal microscopy following DAPI staining. (E) relative expression of the <i>jhe</i> gene, the major gene involved in JH metabolism, in the larval whole body. (F) JH metabolism (JHE activity) assayed by partition assay. Data refer to fourth instar (L4) queen and worker larvae, and two stages in the life cycle of adult workers (nurse and forager). Means ± SEM are represented in all panels, with sample sizes (N) shown above bars, except for panel 2A, where transcripts levels were quantified in single pools of CA-CC complexes. Different letters represent statistical differences. Statistical analyses: (2B, C and D) One-way ANOVA, <i>post-hoc</i> Holm-Sidak test, p≤0.001; (2F) One-way ANOVA, <i>post-hoc</i> Tukey test, p≤0.001; (2E) t-test: p≤0.001.</p>", "links"=>[], "tags"=>["Biochemistry", "hormones", "developmental biology", "Molecular development", "genetics", "Animal genetics", "gene expression", "Molecular genetics", "Molecular cell biology", "Zoology", "Entomology", "jh", "biosynthesis", "pathway", "metabolism", "larvae"], "article_id"=>917896, "categories"=>["Biological Sciences"], "users"=>["Ana Durvalina Bomtorin", "Aline Mackert", "Gustavo Conrado Couto Rosa", "Livia Maria Moda", "Juliana Ramos Martins", "Marcia Maria Gentile Bitondi", "Klaus Hartfelder", "Zilá Luz Paulino Simões"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0086923.g002", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Transcript_levels_of_JH_biosynthesis_pathway_genes_JH_titer_corpora_allata_CA_size_and_JH_metabolism_in_honey_bee_larvae_and_adults_/917896", "title"=>"Transcript levels of JH biosynthesis pathway genes, JH titer, <i>corpora allata</i> (CA) size, and JH metabolism in honey bee larvae and adults.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-29 03:42:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/1366257"], "description"=>"_", "links"=>[], "tags"=>["Biochemistry", "hormones", "developmental biology", "Molecular development", "genetics", "Animal genetics", "gene expression", "Molecular genetics", "Molecular cell biology", "Zoology", "Entomology", "assaying", "jh", "synthesis", "pathway", "metabolism"], "article_id"=>917899, "categories"=>["Biological Sciences"], "users"=>["Ana Durvalina Bomtorin", "Aline Mackert", "Gustavo Conrado Couto Rosa", "Livia Maria Moda", "Juliana Ramos Martins", "Marcia Maria Gentile Bitondi", "Klaus Hartfelder", "Zilá Luz Paulino Simões"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0086923.t002", "stats"=>{"downloads"=>2, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Primers_for_assaying_JH_synthesis_pathway_genes_and_for_a_JH_metabolism_gene_jhe_rp49_was_used_as_reference_gene_/917899", "title"=>"Primers for assaying JH synthesis pathway genes, and for a JH metabolism gene (<i>jhe</i>). <i>rp49</i> was used as reference gene.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-01-29 03:42:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/1366256"], "description"=>"<p>Newly-emerged adult workers were injected with 3 µg <i>mfe</i> dsRNA or GFP dsRNA or left untreated. The bees were collected 7 days after the treatment. (A) <i>mfe</i> transcript levels determined by RT-qPCR using whole body RNA. (B) JH hemolymph titers measured by RIA. (C) <i>jhe</i> transcript levels determined by RT-qPCR using whole body RNA. <i>rp49</i> was used for normalization. Each bar represents the mean ± SEM of independent samples; sample size is shown above bars. Different letters represent statistical differences assessed by One-way ANOVA followed by Tukey's <i>post hoc</i> test; (p≤0.001 in A and C; p = 0.003 in B).</p>", "links"=>[], "tags"=>["Biochemistry", "hormones", "developmental biology", "Molecular development", "genetics", "Animal genetics", "gene expression", "Molecular genetics", "Molecular cell biology", "Zoology", "Entomology", "rnai-mediated", "methyl", "farnesoate", "epoxidase", "genes", "jh", "biosynthesis", "metabolism", "hemolymph"], "article_id"=>917898, "categories"=>["Biological Sciences"], "users"=>["Ana Durvalina Bomtorin", "Aline Mackert", "Gustavo Conrado Couto Rosa", "Livia Maria Moda", "Juliana Ramos Martins", "Marcia Maria Gentile Bitondi", "Klaus Hartfelder", "Zilá Luz Paulino Simões"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0086923.g004", "stats"=>{"downloads"=>1, "page_views"=>44, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_RNAi_mediated_silencing_of_methyl_farnesoate_epoxidase_mfe_gene_function_on_major_control_genes_of_JH_biosynthesis_and_metabolism_and_on_the_hemolymph_JH_titer_/917898", "title"=>"Effects of RNAi-mediated silencing of methyl farnesoate epoxidase (<i>mfe</i>) gene function on major control genes of JH biosynthesis and metabolism and on the hemolymph JH titer.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-29 03:42:56"}
  • {"files"=>["https://ndownloader.figshare.com/files/1366264", "https://ndownloader.figshare.com/files/1366265"], "description"=>"<div><p>Juvenile hormone (JH) controls key events in the honey bee life cycle, <i>viz.</i> caste development and age polyethism. We quantified transcript abundance of 24 genes involved in the JH biosynthetic pathway in the <i>corpora allata-corpora cardiaca</i> (CA-CC) complex. The expression of six of these genes showing relatively high transcript abundance was contrasted with CA size, hemolymph JH titer, as well as JH degradation rates and JH esterase (<i>jhe</i>) transcript levels. Gene expression did not match the contrasting JH titers in queen and worker fourth instar larvae, but <i>jhe</i> transcript abundance and JH degradation rates were significantly lower in queen larvae. Consequently, transcriptional control of JHE is of importance in regulating larval JH titers and caste development. In contrast, the same analyses applied to adult worker bees allowed us inferring that the high JH levels in foragers are due to increased JH synthesis. Upon RNAi-mediated silencing of the methyl farnesoate epoxidase gene (<i>mfe</i>) encoding the enzyme that catalyzes methyl farnesoate-to-JH conversion, the JH titer was decreased, thus corroborating that JH titer regulation in adult honey bees depends on this final JH biosynthesis step. The molecular pathway differences underlying JH titer regulation in larval caste development versus adult age polyethism lead us to propose that <i>mfe</i> and <i>jhe</i> genes be assayed when addressing questions on the role(s) of JH in social evolution.</p></div>", "links"=>[], "tags"=>["Biochemistry", "hormones", "developmental biology", "Molecular development", "genetics", "Animal genetics", "gene expression", "Molecular genetics", "Molecular cell biology", "Zoology", "Entomology", "juvenile", "biosynthesis", "castes"], "article_id"=>917906, "categories"=>["Biological Sciences"], "users"=>["Ana Durvalina Bomtorin", "Aline Mackert", "Gustavo Conrado Couto Rosa", "Livia Maria Moda", "Juliana Ramos Martins", "Marcia Maria Gentile Bitondi", "Klaus Hartfelder", "Zilá Luz Paulino Simões"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0086923.s001", "https://dx.doi.org/10.1371/journal.pone.0086923.s002"], "stats"=>{"downloads"=>0, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Juvenile_Hormone_Biosynthesis_Gene_Expression_in_the_corpora_allata_of_Honey_Bee_Apis_mellifera_L_Female_Castes/917906", "title"=>"Juvenile Hormone Biosynthesis Gene Expression in the <i>corpora allata</i> of Honey Bee (<i>Apis mellifera</i> L.) Female Castes", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-01-29 03:42:56"}

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