Analysis of Escherichia coli Mutants with a Linear Respiratory Chain
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{"title"=>"Analysis of Escherichia coli mutants with a linear respiratory chain", "type"=>"journal", "authors"=>[{"first_name"=>"Sonja", "last_name"=>"Steinsiek", "scopus_author_id"=>"14424567600"}, {"first_name"=>"Stefan", "last_name"=>"Stagge", "scopus_author_id"=>"35590813800"}, {"first_name"=>"Katja", "last_name"=>"Bettenbrock", "scopus_author_id"=>"6603247189"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"373059136", "sgr"=>"84900299526", "pmid"=>"24475268", "scopus"=>"2-s2.0-84900299526", "isbn"=>"10.1371/journal.pone.0087307", "doi"=>"10.1371/journal.pone.0087307", "issn"=>"19326203"}, "id"=>"5fe971f2-7f87-3dc7-a835-a66b229325b7", "abstract"=>"The respiratory chain of E. coli is branched to allow the cells' flexibility to deal with changing environmental conditions. It consists of the NADH:ubiquinone oxidoreductases NADH dehydrogenase I and II, as well as of three terminal oxidases. They differ with respect to energetic efficiency (proton translocation) and their affinity to the different quinone/quinol species and oxygen. In order to analyze the advantages of the branched electron transport chain over a linear one and to assess how usage of the different terminal oxidases determines growth behavior at varying oxygen concentrations, a set of isogenic mutant strains was created, which lack NADH dehydrogenase I as well as two of the terminal oxidases, resulting in strains with a linear respiratory chain. These strains were analyzed in glucose-limited chemostat experiments with defined oxygen supply, adjusting aerobic, anaerobic and different microaerobic conditions. In contrast to the wild-type strain MG1655, the mutant strains produced acetate even under aerobic conditions. Strain TBE032, lacking NADH dehydrogenase I and expressing cytochrome bd-II as sole terminal oxidase, showed the highest acetate formation rate under aerobic conditions. This supports the idea that cytochrome bd-II terminal oxidase is not able to catalyze the efficient oxidation of the quinol pool at higher oxygen conditions, but is functioning mainly under limiting oxygen conditions. Phosphorylation of ArcA, the regulator of the two-component system ArcBA, besides Fnr the main transcription factor for the response towards different oxygen concentrations, was studied. Its phosphorylation pattern was changed in the mutant strains. Dephosphorylation and therefore inactivation of ArcA started at lower aerobiosis levels than in the wild-type strain. Notably, not only the micro- and aerobic metabolism was affected by the mutations, but also the anaerobic metabolism, where the respiratory chain should not be important.", "link"=>"http://www.mendeley.com/research/analysis-escherichia-coli-mutants-linear-respiratory-chain-3", "reader_count"=>33, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Researcher"=>7, "Student > Ph. D. Student"=>19, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Researcher"=>7, "Student > Ph. D. Student"=>19, "Student > Postgraduate"=>1, "Student > Master"=>3, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>3, "Environmental Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>4, "Agricultural and Biological Sciences"=>20, "Medicine and Dentistry"=>1, "Veterinary Science and Veterinary Medicine"=>1, "Chemical Engineering"=>1, "Computer Science"=>1, "Immunology and Microbiology"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>3}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>20}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}, "Environmental Science"=>{"Environmental Science"=>1}, "Chemical Engineering"=>{"Chemical Engineering"=>1}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "reader_count_by_country"=>{"Belgium"=>1, "United Kingdom"=>1, "Australia"=>1}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1361644"], "description"=>"<p>The transcription pattern was analyzed in cells from glucose-limited continuous cultures and normalized to the reference genes <i>recA, rpoD</i> and <i>ybhC</i> and to the expression under anaerobic conditions. <i>aceA</i>: isocitrate lyase, <i>ackA</i>: acetate kinase, <i>poxB</i>: pyruvate oxidase, <i>acs</i>: acetyl-CoA synthetase.</p>", "links"=>[], "tags"=>["genetics", "gene expression", "microbiology", "Bacterial pathogens", "Escherichia coli", "bacteriology", "Bacterial biochemistry", "Bacterial physiology", "Applied microbiology", "Microbial control", "Microbial metabolism", "Microbial physiology", "genes", "coding", "enzymes", "acetate", "metabolism", "mg1655", "mutant", "tbe029"], "article_id"=>914113, "categories"=>["Biological Sciences"], "users"=>["Sonja Steinsiek", "Stefan Stagge", "Katja Bettenbrock"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0087307.g005", "stats"=>{"downloads"=>3, "page_views"=>27, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Relative_expression_of_genes_coding_for_enzymes_of_acetate_metabolism_in_E_coli_MG1655_4a_and_mutant_strain_TBE029_4b_/914113", "title"=>"Relative expression of genes coding for enzymes of acetate metabolism in <i>E. coli</i> MG1655 (4a) and mutant strain TBE029 (4b).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-27 03:21:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/1361646"], "description"=>"<p>TBE029: Δ<i>nuoB</i>. A few data points do not seem to fit and might only be measuring inaccuracies. Nevertheless all values are displayed here as it is difficult to distinguish between measurement errors and variance in ArcA phosphorylation.</p>", "links"=>[], "tags"=>["genetics", "gene expression", "microbiology", "Bacterial pathogens", "Escherichia coli", "bacteriology", "Bacterial biochemistry", "Bacterial physiology", "Applied microbiology", "Microbial control", "Microbial metabolism", "Microbial physiology", "phosphorylation", "mg1655", "mutant", "tbe029"], "article_id"=>914114, "categories"=>["Biological Sciences"], "users"=>["Sonja Steinsiek", "Stefan Stagge", "Katja Bettenbrock"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0087307.g006", "stats"=>{"downloads"=>3, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ArcA_phosphorylation_in_E_coli_MG1655_and_mutant_strain_TBE029_over_the_aerobiosis_/914114", "title"=>"ArcA phosphorylation in <i>E. coli</i> MG1655 and mutant strain TBE029 over the aerobiosis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-27 03:21:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/1361647"], "description"=>"<p>TBE031: Δ<i>nuoB</i>, Δ<i>cydB</i>, Δ<i>appB</i>. The transcription pattern was analyzed in cells from glucose-limited continuous cultures and normalized to the reference genes <i>recA, rpoD</i> and <i>ybhC</i> and to the expression of the wild-type strain under anaerobic conditions. <i>ndh</i>: NADH dehydrogenase II, <i>cyoA</i>: subunit of cytochrome oxidase <i>bo, gltA</i>: citrate synthase, <i>sucA</i>: subunit of 2-oxoglutarate dehydrogenase, <i>sdhD</i>: subunit of succinate dehydrogenase.</p>", "links"=>[], "tags"=>["genetics", "gene expression", "microbiology", "Bacterial pathogens", "Escherichia coli", "bacteriology", "Bacterial biochemistry", "Bacterial physiology", "Applied microbiology", "Microbial control", "Microbial metabolism", "Microbial physiology", "genes", "coding", "respiratory", "enzymes", "tca", "tbe031"], "article_id"=>914115, "categories"=>["Biological Sciences"], "users"=>["Sonja Steinsiek", "Stefan Stagge", "Katja Bettenbrock"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0087307.g007", "stats"=>{"downloads"=>1, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Relative_expression_of_genes_coding_for_respiratory_enzymes_6a_and_TCA_cycle_enzymes_6b_in_strain_TBE031_Cyt_bo_/914115", "title"=>"Relative expression of genes coding for respiratory enzymes (6a) and TCA cycle enzymes (6b) in strain TBE031 (Cyt <i>bo</i>).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-27 03:21:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/1361648"], "description"=>"<p>TBE029: Δ<i>nuoB</i>; TBE031: Δ<i>nuoB</i>, Δ<i>cydB</i>, Δ<i>appB</i>; TBE032: Δ<i>nuoB</i>, Δ<i>cydB</i>, Δ<i>cyoB</i>; TBE042: Δ<i>nuoB</i>, Δ<i>cyoB</i>, Δ<i>appB</i>.</p>", "links"=>[], "tags"=>["genetics", "gene expression", "microbiology", "Bacterial pathogens", "Escherichia coli", "bacteriology", "Bacterial biochemistry", "Bacterial physiology", "Applied microbiology", "Microbial control", "Microbial metabolism", "Microbial physiology", "phosphorylation", "respiratory", "mutant", "strains"], "article_id"=>914116, "categories"=>["Biological Sciences"], "users"=>["Sonja Steinsiek", "Stefan Stagge", "Katja Bettenbrock"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0087307.g008", "stats"=>{"downloads"=>3, "page_views"=>24, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_ArcA_phosphorylation_in_E_coli_respiratory_mutant_strains_over_the_aerobiosis_/914116", "title"=>"ArcA phosphorylation in <i>E. coli</i> respiratory mutant strains over the aerobiosis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-27 03:21:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/1361649"], "description"=>"<p>TBE032: Δ<i>nuoB</i>, Δ<i>cyoB</i>, Δ<i>cydB</i>. The transcription pattern was analyzed in cells from glucose-limited continuous cultures and normalized to the reference genes <i>recA, rpoD</i> and <i>ybhC</i> and to the expression of the wild-type strain under anaerobic conditions. <i>ndh</i>: NADH dehydrogenase II, <i>appC</i>: subunit of cytochrome oxidase <i>bd-II, gltA</i>: citrate synthase, <i>sucA</i>: subunit of 2-oxoglutarate dehydrogenase, <i>sdhD</i>: subunit of succinate dehydrogenase.</p>", "links"=>[], "tags"=>["genetics", "gene expression", "microbiology", "Bacterial pathogens", "Escherichia coli", "bacteriology", "Bacterial biochemistry", "Bacterial physiology", "Applied microbiology", "Microbial control", "Microbial metabolism", "Microbial physiology", "genes", "coding", "respiratory", "enzymes", "tca", "tbe032"], "article_id"=>914117, "categories"=>["Biological Sciences"], "users"=>["Sonja Steinsiek", "Stefan Stagge", "Katja Bettenbrock"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0087307.g009", "stats"=>{"downloads"=>5, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Relative_expression_of_genes_coding_for_respiratory_enzymes_8a_and_TCA_cycle_enzymes_8b_in_strain_TBE032_Cyt_bd_II_/914117", "title"=>"Relative expression of genes coding for respiratory enzymes (8a) and TCA cycle enzymes (8b) in strain TBE032 (Cyt <i>bd-II</i>).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-27 03:21:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/1361650"], "description"=>"<p>TBE042: Δ<i>nuoB</i>, Δ<i>cyoB</i>, Δ<i>appB</i>. The transcription pattern was analyzed in cells from glucose-limited continuous cultures and normalized to the reference genes <i>recA, rpoD</i> and <i>ybhC</i> and to the expression of the wild-type strain under anaerobic conditions. <i>ndh</i>: NADH dehydrogenase II, <i>cydA</i>: subunit of cytochrome oxidase <i>bd-I, gltA</i>: citrate synthase, <i>sucA</i>: subunit of 2-oxoglutarate dehydrogenase, <i>sdhD</i>: subunit of succinate dehydrogenase.</p>", "links"=>[], "tags"=>["genetics", "gene expression", "microbiology", "Bacterial pathogens", "Escherichia coli", "bacteriology", "Bacterial biochemistry", "Bacterial physiology", "Applied microbiology", "Microbial control", "Microbial metabolism", "Microbial physiology", "genes", "coding", "respiratory", "enzymes", "tca", "tbe042"], "article_id"=>914118, "categories"=>["Biological Sciences"], "users"=>["Sonja Steinsiek", "Stefan Stagge", "Katja Bettenbrock"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0087307.g010", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Relative_expression_of_genes_coding_for_respiratory_enzymes_9a_and_TCA_cycle_enzymes_9b_in_strain_TBE042_Cyt_bd_I_/914118", "title"=>"Relative expression of genes coding for respiratory enzymes (9a) and TCA cycle enzymes (9b) in strain TBE042 (Cyt <i>bd-I</i>).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-27 03:21:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/1361651"], "description"=>"<p>By-product formation rates are calculated per hour and gram dry cell weight [mmol*g<sup>−1</sup>*h<sup>−1</sup>]. TBE029: Δ<i>nuoB</i>; TBE031: Δ<i>nuoB</i>, Δ<i>cydB</i>, Δ<i>appB</i>; TBE032: Δ<i>nuoB</i>, Δ<i>cydB</i>, Δ<i>cyoB</i>; TBE042: Δ<i>nuoB</i>, Δ<i>cyoB</i>, Δ<i>appB</i>.</p>", "links"=>[], "tags"=>["genetics", "gene expression", "microbiology", "Bacterial pathogens", "Escherichia coli", "bacteriology", "Bacterial biochemistry", "Bacterial physiology", "Applied microbiology", "Microbial control", "Microbial metabolism", "Microbial physiology", "rates", "glucose-limited", "chemostat", "cultures", "aerobiosis"], "article_id"=>914119, "categories"=>["Biological Sciences"], "users"=>["Sonja Steinsiek", "Stefan Stagge", "Katja Bettenbrock"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0087307.t003", "stats"=>{"downloads"=>6, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Acetate_l_formation_rates_from_glucose_limited_chemostat_cultures_at_different_aerobiosis_levels_/914119", "title"=>"Acetate l formation rates from glucose-limited chemostat cultures at different aerobiosis levels.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-01-27 03:21:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/1361652"], "description"=>"_", "links"=>[], "tags"=>["genetics", "gene expression", "microbiology", "Bacterial pathogens", "Escherichia coli", "bacteriology", "Bacterial biochemistry", "Bacterial physiology", "Applied microbiology", "Microbial control", "Microbial metabolism", "Microbial physiology", "genes", "primer", "pairs", "Real-time"], "article_id"=>914120, "categories"=>["Biological Sciences"], "users"=>["Sonja Steinsiek", "Stefan Stagge", "Katja Bettenbrock"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0087307.t002", "stats"=>{"downloads"=>4, "page_views"=>2, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_List_of_genes_and_primer_pairs_for_real_Time_RT_PCR_/914120", "title"=>"List of genes and primer pairs for real-Time RT-PCR", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-01-27 03:21:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/1361653"], "description"=>"_", "links"=>[], "tags"=>["genetics", "gene expression", "microbiology", "Bacterial pathogens", "Escherichia coli", "bacteriology", "Bacterial biochemistry", "Bacterial physiology", "Applied microbiology", "Microbial control", "Microbial metabolism", "Microbial physiology"], "article_id"=>914121, "categories"=>["Biological Sciences"], "users"=>["Sonja Steinsiek", "Stefan Stagge", "Katja Bettenbrock"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0087307.t001", "stats"=>{"downloads"=>3, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Strains_used_in_this_work_/914121", "title"=>"Strains used in this work.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-01-27 03:21:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/1361654"], "description"=>"<p>By-product formation rates are calculated per hour and gram dry cell weight [mmol*g<sup>−1</sup>*h<sup>−1</sup>]. TBE029: Δ<i>nuoB</i>; TBE031: Δ<i>nuoB</i>, Δ<i>cydB</i>, Δ<i>appB</i>; TBE032: Δ<i>nuoB</i>, Δ<i>cydB</i>, Δ<i>cyoB</i>; TBE042: Δ<i>nuoB</i>, Δ<i>cyoB</i>, Δ<i>appB</i>.</p>", "links"=>[], "tags"=>["genetics", "gene expression", "microbiology", "Bacterial pathogens", "Escherichia coli", "bacteriology", "Bacterial biochemistry", "Bacterial physiology", "Applied microbiology", "Microbial control", "Microbial metabolism", "Microbial physiology", "rates", "glucose-limited", "chemostat", "cultures", "aerobiosis"], "article_id"=>914122, "categories"=>["Biological Sciences"], "users"=>["Sonja Steinsiek", "Stefan Stagge", "Katja Bettenbrock"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0087307.t004", "stats"=>{"downloads"=>5, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Ethanol_formation_rates_from_glucose_limited_chemostat_cultures_at_different_aerobiosis_levels_/914122", "title"=>"Ethanol formation rates from glucose-limited chemostat cultures at different aerobiosis levels.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-01-27 03:21:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/1361655", "https://ndownloader.figshare.com/files/1361656"], "description"=>"<div><p>The respiratory chain of <i>E. coli</i> is branched to allow the cells' flexibility to deal with changing environmental conditions. It consists of the NADH:ubiquinone oxidoreductases NADH dehydrogenase I and II, as well as of three terminal oxidases. They differ with respect to energetic efficiency (proton translocation) and their affinity to the different quinone/quinol species and oxygen. In order to analyze the advantages of the branched electron transport chain over a linear one and to assess how usage of the different terminal oxidases determines growth behavior at varying oxygen concentrations, a set of isogenic mutant strains was created, which lack NADH dehydrogenase I as well as two of the terminal oxidases, resulting in strains with a linear respiratory chain. These strains were analyzed in glucose-limited chemostat experiments with defined oxygen supply, adjusting aerobic, anaerobic and different microaerobic conditions. In contrast to the wild-type strain MG1655, the mutant strains produced acetate even under aerobic conditions. Strain TBE032, lacking NADH dehydrogenase I and expressing cytochrome <i>bd-II</i> as sole terminal oxidase, showed the highest acetate formation rate under aerobic conditions. This supports the idea that cytochrome <i>bd-II</i> terminal oxidase is not able to catalyze the efficient oxidation of the quinol pool at higher oxygen conditions, but is functioning mainly under limiting oxygen conditions. Phosphorylation of ArcA, the regulator of the two-component system ArcBA, besides Fnr the main transcription factor for the response towards different oxygen concentrations, was studied. Its phosphorylation pattern was changed in the mutant strains. Dephosphorylation and therefore inactivation of ArcA started at lower aerobiosis levels than in the wild-type strain. Notably, not only the micro- and aerobic metabolism was affected by the mutations, but also the anaerobic metabolism, where the respiratory chain should not be important.</p></div>", "links"=>[], "tags"=>["genetics", "gene expression", "microbiology", "Bacterial pathogens", "Escherichia coli", "bacteriology", "Bacterial biochemistry", "Bacterial physiology", "Applied microbiology", "Microbial control", "Microbial metabolism", "Microbial physiology", "mutants", "linear", "respiratory"], "article_id"=>914123, "categories"=>["Biological Sciences"], "users"=>["Sonja Steinsiek", "Stefan Stagge", "Katja Bettenbrock"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0087307.s001", "https://dx.doi.org/10.1371/journal.pone.0087307.s002"], "stats"=>{"downloads"=>8, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Analysis_of_Escherichia_coli_Mutants_with_a_Linear_Respiratory_Chain/914123", "title"=>"Analysis of <i>Escherichia coli</i> Mutants with a Linear Respiratory Chain", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-01-27 03:21:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/1361640"], "description"=>"<p>The correlation between the percentage of oxygen in the input gas and the acetate production of the different strains was analyzed in bioreactor experiments. While MG1655 displays a linear inverse correlation with no longer detectable acetate production at about 4.5% oxygen in the input gas the mutant strains showed no linear correlations and acetate production was not completely abolished even at higher O<sub>2</sub> input rates.</p>", "links"=>[], "tags"=>["genetics", "gene expression", "microbiology", "Bacterial pathogens", "Escherichia coli", "bacteriology", "Bacterial biochemistry", "Bacterial physiology", "Applied microbiology", "Microbial control", "Microbial metabolism", "Microbial physiology", "scales", "aof", "mg1655", "mutant"], "article_id"=>914108, "categories"=>["Biological Sciences"], "users"=>["Sonja Steinsiek", "Stefan Stagge", "Katja Bettenbrock"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0087307.g001", "stats"=>{"downloads"=>4, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Aerobiosis_scales_aof_MG1655_and_the_mutant_strains_/914108", "title"=>"Aerobiosis scales aof MG1655 and the mutant strains.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-27 03:21:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/1361641"], "description"=>"<p>100% aerobiosis corresponds to the minimal oxygen concentration in glucose-limited cultures to prevent acetate formation in MG1655. TBE029: Δ<i>nuoB</i>.</p>", "links"=>[], "tags"=>["genetics", "gene expression", "microbiology", "Bacterial pathogens", "Escherichia coli", "bacteriology", "Bacterial biochemistry", "Bacterial physiology", "Applied microbiology", "Microbial control", "Microbial metabolism", "Microbial physiology", "und", "ethanol", "rates", "concentrations", "glucose-limited", "cultures", "wild-type", "mg1655", "tbe029"], "article_id"=>914109, "categories"=>["Biological Sciences"], "users"=>["Sonja Steinsiek", "Stefan Stagge", "Katja Bettenbrock"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0087307.g002", "stats"=>{"downloads"=>4, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Acetate_und_ethanol_formation_rates_at_different_oxygen_concentrations_in_glucose_limited_cultures_for_the_wild_type_strain_MG1655_1a_and_strain_TBE029_1b_/914109", "title"=>"Acetate und ethanol formation rates at different oxygen concentrations in glucose-limited cultures for the wild-type strain MG1655 (1a) and strain TBE029 (1b).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-27 03:21:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/1361642"], "description"=>"<p>Transcription of NADH dehydrogenase I and II and of the cytochrome oxidases <i>bo</i>, <i>bd-I</i> and <i>bd-II</i>. The transcription pattern was analyzed in cells from glucose-limited continuous cultures and normalized to the reference genes <i>recA, rpoD</i> and <i>ybhC</i> and to the expression of the wild-type strain under anaerobic conditions. TBE029: Δ<i>nuoB</i>. <i>ndh</i>: NADH dehydrogenase II, <i>nuoN</i>: NADH dehydrogenase I, <i>cyoA</i>: subunit of cytochrome oxidase <i>bo</i>, <i>cydA</i>: subunit of cytochrome oxidase <i>bd-I</i>, <i>appC</i>: subunit of cytochrome oxidase <i>bd-II</i>.</p>", "links"=>[], "tags"=>["genetics", "gene expression", "microbiology", "Bacterial pathogens", "Escherichia coli", "bacteriology", "Bacterial biochemistry", "Bacterial physiology", "Applied microbiology", "Microbial control", "Microbial metabolism", "Microbial physiology", "genes", "coding", "respiratory", "enzymes", "mg1655", "mutant", "tbe029"], "article_id"=>914110, "categories"=>["Biological Sciences"], "users"=>["Sonja Steinsiek", "Stefan Stagge", "Katja Bettenbrock"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0087307.g003", "stats"=>{"downloads"=>2, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Relative_expression_of_genes_coding_for_respiratory_enzymes_in_E_coli_MG1655_2a_and_mutant_strain_TBE029_2b_/914110", "title"=>"Relative expression of genes coding for respiratory enzymes in <i>E. coli</i> MG1655 (2a) and mutant strain TBE029 (2b).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-27 03:21:21"}
  • {"files"=>["https://ndownloader.figshare.com/files/1361643"], "description"=>"<p>Transcription of citrate synthase, 2-oxoglutarate dehydrogenase and succinate dehydrogenase. The transcription pattern was analyzed in cells from glucose-limited continuous cultures and normalized to the reference genes <i>recA, rpoD</i> and <i>ybhC</i> and to the expression of the wild-type strain under anaerobic conditions. TBE029: Δ<i>nuoB</i>. <i>gltA</i>: citrate synthase, <i>sucA</i>: subunit of 2-oxoglutarate dehydrogenase, <i>sdhD</i>: subunit of succinate dehydrogenase.</p>", "links"=>[], "tags"=>["genetics", "gene expression", "microbiology", "Bacterial pathogens", "Escherichia coli", "bacteriology", "Bacterial biochemistry", "Bacterial physiology", "Applied microbiology", "Microbial control", "Microbial metabolism", "Microbial physiology", "genes", "coding", "enzymes", "tca", "mg1655", "mutant", "tbe029"], "article_id"=>914111, "categories"=>["Biological Sciences"], "users"=>["Sonja Steinsiek", "Stefan Stagge", "Katja Bettenbrock"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0087307.g004", "stats"=>{"downloads"=>1, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Relative_expression_of_genes_coding_for_enzymes_of_the_TCA_cycle_in_E_coli_MG1655_3a_and_mutant_strain_TBE029_3b_/914111", "title"=>"Relative expression of genes coding for enzymes of the TCA cycle in <i>E. coli</i> MG1655 (3a) and mutant strain TBE029 (3b).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-01-27 03:21:21"}

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Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[306, 482]}, {"subject_area"=>"/Physical sciences/Chemistry", "average_usage"=>[262]}]}
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