Mobile Insertion Cassette Elements Found in Small Non-Transmissible Plasmids in Proteeae May Explain qnrD Mobilization
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Mobile insertion cassette elements found in small non-transmissible plasmids in Proteeae may explain qnrD mobilization", "type"=>"journal", "authors"=>[{"first_name"=>"Thomas", "last_name"=>"Guillard", "scopus_author_id"=>"26431954200"}, {"first_name"=>"Antoine", "last_name"=>"Grillon", "scopus_author_id"=>"36155311100"}, {"first_name"=>"Christophe", "last_name"=>"De Champs", "scopus_author_id"=>"7006641440"}, {"first_name"=>"Céline", "last_name"=>"Cartier", "scopus_author_id"=>"56054648300"}, {"first_name"=>"Janick", "last_name"=>"Madoux", "scopus_author_id"=>"13103958700"}, {"first_name"=>"Béatrice", "last_name"=>"Berçot", "scopus_author_id"=>"11640390500"}, {"first_name"=>"Anne Laure", "last_name"=>"Lebreil", "scopus_author_id"=>"55922533100"}, {"first_name"=>"Alain", "last_name"=>"Lozniewski", "scopus_author_id"=>"7004067627"}, {"first_name"=>"Jacques", "last_name"=>"Riahi", "scopus_author_id"=>"11641419900"}, {"first_name"=>"Véronique", "last_name"=>"Vernet-Garnier", "scopus_author_id"=>"6507781701"}, {"first_name"=>"Emmanuelle", "last_name"=>"Cambau", "scopus_author_id"=>"7003706122"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84895094867", "doi"=>"10.1371/journal.pone.0087801", "pui"=>"372510286", "issn"=>"19326203", "pmid"=>"24504382", "sgr"=>"84895094867"}, "id"=>"28b9e0b1-32dc-3db6-83ad-4ae9443121a7", "abstract"=>"qnrD is a plasmid mediated quinolone resistance gene from unknown origin, recently described in Enterobacteriaceae. It encodes a pentapeptide repeat protein 36-60% different from the other Qnr (A, B, C, S and VC). Since most qnrD-positive strains were described as strains belonging to Proteus or Providencia genera, we hypothesized that qnrD originated in Proteeae before disseminating to other enterobacterial species. We screened 317 strains of Proteeae for qnrD and its genetic support by PCR. For all the seven qnrD-positive strains (4 Proteus mirabilis, 1 Proteus vulgaris and 2 Providencia rettgeri) the gene was carried onto a small non-transmissible plasmid, contrarily to other qnr genes that are usually carried onto large multi-resistant plasmids. Nucleotide sequences of the qnrD-bearing plasmids were 96% identical. Plasmids contained 3 ORFs apart from qnrD and belonged to an undescribed incompatibility group. Only one plasmid, in P. vulgaris, was slightly different with a 1,568-bp insertion between qnrD and its promoter, leading to absence of quinolone resistance. We sought for similar plasmids in 15 reference strains of Proteeae, but which were tested negative for qnrD, and found a 48% identical plasmid (pVERM) in Providencia vermicola. In order to explain how qnrD could have been inserted into such native plasmid, we sought for gene mobilization structures. qnrD was found to be located within a mobile insertion cassette (mic) element which sequences are similar to one mic also found in pVERM. Our conclusions are that (i) the small non-transmissible qnrD-plasmids described here may result from the recombination between an as-yet-unknown progenitor of qnrD and pVERM, (ii) these plasmids are maintained in Proteeae being a qnrD reservoir (iii) the mic element may explain qnrD mobilization from non-transmissible plasmids to mobilizable or conjugative plasmids from other Enterobacteriaceae, (iv) they can recombined with larger multiresistant plasmids conjugated in Proteeae.", "link"=>"http://www.mendeley.com/research/mobile-insertion-cassette-elements-found-small-nontransmissible-plasmids-proteeae-explain-qnrd-mobil", "reader_count"=>16, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Student > Doctoral Student"=>2, "Researcher"=>1, "Student > Ph. D. Student"=>5, "Other"=>2, "Student > Master"=>3, "Student > Bachelor"=>1, "Professor"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Student > Doctoral Student"=>2, "Researcher"=>1, "Student > Ph. D. Student"=>5, "Other"=>2, "Student > Master"=>3, "Student > Bachelor"=>1, "Professor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>4, "Medicine and Dentistry"=>2, "Veterinary Science and Veterinary Medicine"=>1, "Chemistry"=>1, "Immunology and Microbiology"=>4}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Chemistry"=>{"Chemistry"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>4}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Unspecified"=>{"Unspecified"=>2}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "reader_count_by_country"=>{"Brazil"=>1, "France"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84895094867"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84895094867?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84895094867&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84895094867&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84895094867", "dc:identifier"=>"SCOPUS_ID:84895094867", "eid"=>"2-s2.0-84895094867", "dc:title"=>"Mobile insertion cassette elements found in small non-transmissible plasmids in Proteeae may explain qnrD mobilization", "dc:creator"=>"Guillard T.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"9", "prism:issueIdentifier"=>"2", "prism:pageRange"=>nil, "prism:coverDate"=>"2014-02-04", "prism:coverDisplayDate"=>"4 February 2014", "prism:doi"=>"10.1371/journal.pone.0087801", "citedby-count"=>"27", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Hopital Robert Debre CHU de Reims", "affiliation-city"=>"Reims", "affiliation-country"=>"France"}, {"@_fa"=>"true", "affilname"=>"Université de Reims Champagne-Ardenne", "affiliation-city"=>"Reims", "affiliation-country"=>"France"}, {"@_fa"=>"true", "affilname"=>"Universite Paris 7- Denis Diderot", "affiliation-city"=>"Paris", "affiliation-country"=>"France"}], "pubmed-id"=>"24504382", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e87801", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0087801", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Journal Comments | Further Information

Counter

  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"27", "xml_views"=>"4", "html_views"=>"92"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"12", "xml_views"=>"3", "html_views"=>"66"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"4", "html_views"=>"86"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"60"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"47"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"10"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"37"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"30"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"36"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"34"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"51"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"15", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"24"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"10"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"14", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"50"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"18"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"3", "html_views"=>"5"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"12"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"3", "html_views"=>"4"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"1"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"4"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"2", "html_views"=>"3"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"11", "year"=>"2019", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"12", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"1", "year"=>"2020", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"2", "year"=>"2020", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"4"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1375231"], "description"=>"<p>A. Sequence analysis of the mic element including <i>qnrD</i> from the sequence of the plasmid pRS12–78, which contains a 1,603 bp insert (ORF5) upstream from <i>qnrD</i>. The nucleotide numbering is shown on the left-hand side. Putative -35, -10 promoter regions as well as the ribosome binding site (RBS) and the putative transcription start sites (+1) of <i>qnrD</i> are in bold. The start and the stop codons of <i>qnrD</i> (in blue) and of the ORF5 (in magenta) are underlined. For the termination site of ORF5, two black arrows indicate inverted repeats, and the string of “A’s” is boxed. The inverted repeats (IRL and IRR) bracketing the mic element are boxed as arrows shaded in grey. In pRS12–78, <i>qnrD</i> is far from its own promoter due to the insertion of ORF5. B. Comparison of the mic elements found in the plasmid, pDIJ09–518, pRS12–78, pVERM and in p2007057. Open reading frames (ORFs) are indicated by horizontal arrows. White arrows are identical ORFs harbored by the different plasmids beyond the mic. Red arrows are identical ORFs harbored by the different plasmids within the mic. The right and left inverted repeats (IRR and IRL) are indicated as black triangles with the duplication sites (CA). Nucleotide sequences of the IRL and IRR are outlined and the nucleotides which differed between them are in bold type and underlined. Asterisks show nucleotide differences of IRs with regard to those in pDIJ09–518a.</p>", "links"=>[], "tags"=>["Evolutionary biology", "Evolutionary genetics", "genetics", "Genetic mutation", "population genetics", "microbiology", "Bacterial pathogens", "bacteriology", "Medical microbiology", "Infectious diseases", "Bacterial diseases", "Enterobacter infections", "insertion", "cassette"], "article_id"=>925391, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Thomas Guillard", "Antoine Grillon", "Christophe de Champs", "Céline Cartier", "Janick Madoux", "Béatrice Berçot", "Anne-Laure Lebreil", "Alain Lozniewski", "Jacques Riahi", "Véronique Vernet-Garnier", "Emmanuelle Cambau"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0087801.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_qnrD_is_located_within_a_mobile_insertion_cassette_mic_element_/925391", "title"=>"<i>qnrD</i> is located within a mobile insertion cassette (mic) element.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-04 03:26:59"}
  • {"files"=>["https://ndownloader.figshare.com/files/1375232"], "description"=>"<p> Comparison of the plasmid structures of pDIJ09-518a from <i>P. rettgeri</i>, pVERM from <i>P. vermicola</i>, p2007057 from <i>S. enterica</i> Bovismorbificans, pIGC156 from <i>E</i>. <i>coli</i> and pSC101 from <i>S. enterica</i> Typhimurium. The open reading frames are shown as arrows with the arrowhead indicating the direction of transcription. Red color is used to shade arrows in the <i>qnrD</i>-mic element. Grey shading shows the areas of homology between the plasmids.</p>", "links"=>[], "tags"=>["Evolutionary biology", "Evolutionary genetics", "genetics", "Genetic mutation", "population genetics", "microbiology", "Bacterial pathogens", "bacteriology", "Medical microbiology", "Infectious diseases", "Bacterial diseases", "Enterobacter infections", "emerged", "disseminated", "mic", "elements", "recombination", "pverm"], "article_id"=>925392, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Thomas Guillard", "Antoine Grillon", "Christophe de Champs", "Céline Cartier", "Janick Madoux", "Béatrice Berçot", "Anne-Laure Lebreil", "Alain Lozniewski", "Jacques Riahi", "Véronique Vernet-Garnier", "Emmanuelle Cambau"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0087801.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_qnrD_may_have_emerged_and_disseminated_from_Proteeae_to_Enterobacteriaceae_using_mic_elements_and_a_recombination_event_with_the_native_PVERM_plasmid_/925392", "title"=>"<i>qnrD</i> may have emerged and disseminated from <i>Proteeae</i> to <i>Enterobacteriaceae</i> using mic elements and a recombination event with the native PVERM plasmid.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-04 03:26:59"}
  • {"files"=>["https://ndownloader.figshare.com/files/1375233"], "description"=>"a<p>Determined by agarose gel electrophoresis of plasmids extracted from the reference strains of <i>Proteeae</i> using the Kieser method. Control plasmids for size were pIP55 (130 kb), pIP135 (70.4 kb) and pGHS09-09 (2.6 kb).;</p><p>−, not done.</p>", "links"=>[], "tags"=>["Evolutionary biology", "Evolutionary genetics", "genetics", "Genetic mutation", "population genetics", "microbiology", "Bacterial pathogens", "bacteriology", "Medical microbiology", "Infectious diseases", "Bacterial diseases", "Enterobacter infections", "strains", "harbored", "plasmids"], "article_id"=>925393, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Thomas Guillard", "Antoine Grillon", "Christophe de Champs", "Céline Cartier", "Janick Madoux", "Béatrice Berçot", "Anne-Laure Lebreil", "Alain Lozniewski", "Jacques Riahi", "Véronique Vernet-Garnier", "Emmanuelle Cambau"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0087801.t001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Reference_strains_of_Proteeae_harbored_native_plasmids_but_no_qnr_genes_/925393", "title"=>"Reference strains of <i>Proteeae</i> harbored native plasmids but no <i>qnr</i> genes.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-02-04 03:26:59"}
  • {"files"=>["https://ndownloader.figshare.com/files/1375235"], "description"=>"<p>Epidemiological findings of the 7 <i>qnrD</i>-positive strains detected among 317 clinical isolates of <i>Proteeae</i>.</p>", "links"=>[], "tags"=>["Evolutionary biology", "Evolutionary genetics", "genetics", "Genetic mutation", "population genetics", "microbiology", "Bacterial pathogens", "bacteriology", "Medical microbiology", "Infectious diseases", "Bacterial diseases", "Enterobacter infections", "findings", "strains", "317", "isolates"], "article_id"=>925395, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Thomas Guillard", "Antoine Grillon", "Christophe de Champs", "Céline Cartier", "Janick Madoux", "Béatrice Berçot", "Anne-Laure Lebreil", "Alain Lozniewski", "Jacques Riahi", "Véronique Vernet-Garnier", "Emmanuelle Cambau"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0087801.t002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Epidemiological_findings_of_the_7_qnrD_positive_strains_detected_among_317_clinical_isolates_of_Proteeae_/925395", "title"=>"Epidemiological findings of the 7 <i>qnrD</i>-positive strains detected among 317 clinical isolates of <i>Proteeae</i>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-02-04 03:26:59"}
  • {"files"=>["https://ndownloader.figshare.com/files/1375236"], "description"=>"<p>NAL, Nalidixic acid; NOR, Norfloxacin; CIP, Ciprofloxacin; OFX, Ofloxacin; LVX, Levofloxacin; MXF, Moxifloxacin; ND, no data.</p>a<p>MICs reported by Cavaco <i>et al.</i><a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087801#pone.0087801-Jacoby1\" target=\"_blank\">[7]</a>.</p>", "links"=>[], "tags"=>["Evolutionary biology", "Evolutionary genetics", "genetics", "Genetic mutation", "population genetics", "microbiology", "Bacterial pathogens", "bacteriology", "Medical microbiology", "Infectious diseases", "Bacterial diseases", "Enterobacter infections", "conferred", "plasmids", "inhibitory", "concentrations", "quinolones", "transformants", "strains"], "article_id"=>925396, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Thomas Guillard", "Antoine Grillon", "Christophe de Champs", "Céline Cartier", "Janick Madoux", "Béatrice Berçot", "Anne-Laure Lebreil", "Alain Lozniewski", "Jacques Riahi", "Véronique Vernet-Garnier", "Emmanuelle Cambau"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0087801.t003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Resistance_conferred_by_qnrD_bearing_plasmids_as_measured_by_determination_of_minimal_inhibitory_concentrations_MIC_of_quinolones_on_transformants_with_regard_to_parental_strains_of_Proteeae_/925396", "title"=>"Resistance conferred by <i>qnrD-</i>bearing plasmids as measured by determination of minimal inhibitory concentrations (MIC) of quinolones on transformants with regard to parental strains of <i>Proteeae</i>.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-02-04 03:26:59"}
  • {"files"=>["https://ndownloader.figshare.com/files/1375237", "https://ndownloader.figshare.com/files/1375238"], "description"=>"<div><p><i>qnrD</i> is a plasmid mediated quinolone resistance gene from unknown origin, recently described in <i>Enterobacteriaceae</i>. It encodes a pentapeptide repeat protein 36–60% different from the other Qnr (A, B, C, S and VC). Since most <i>qnrD</i>-positive strains were described as strains belonging to <i>Proteus</i> or <i>Providencia</i> genera, we hypothesized that <i>qnrD</i> originated in <i>Proteeae</i> before disseminating to other enterobacterial species. We screened 317 strains of <i>Proteeae</i> for <i>qnrD</i> and its genetic support by PCR. For all the seven <i>qnrD-</i>positive strains (4 <i>Proteus mirabilis,</i> 1 <i>Proteus vulgaris</i> and 2 <i>Providencia rettgeri</i>) the gene was carried onto a small non-transmissible plasmid, contrarily to other <i>qnr</i> genes that are usually carried onto large multi-resistant plasmids. Nucleotide sequences of the <i>qnrD</i>-bearing plasmids were 96% identical. Plasmids contained 3 ORFs apart from <i>qnrD</i> and belonged to an undescribed incompatibility group. Only one plasmid, in <i>P. vulgaris</i>, was slightly different with a 1,568-bp insertion between <i>qnrD</i> and its promoter, leading to absence of quinolone resistance. We sought for similar plasmids in 15 reference strains of <i>Proteeae</i>, but which were tested negative for <i>qnrD</i>, and found a 48% identical plasmid (pVERM) in <i>Providencia vermicola</i>. In order to explain how <i>qnrD</i> could have been inserted into such native plasmid, we sought for gene mobilization structures. <i>qnrD</i> was found to be located within a mobile insertion cassette (mic) element which sequences are similar to one mic also found in pVERM. Our conclusions are that (i) the small non-transmissible <i>qnrD</i>-plasmids described here may result from the recombination between an as-yet-unknown progenitor of <i>qnrD</i> and pVERM, (ii) these plasmids are maintained in <i>Proteeae</i> being a <i>qnrD</i> reservoir (iii) the mic element may explain <i>qnrD</i> mobilization from non-transmissible plasmids to mobilizable or conjugative plasmids from other <i>Enterobacteriaceae</i>, (iv) they can recombined with larger multiresistant plasmids conjugated in <i>Proteeae.</i></p></div>", "links"=>[], "tags"=>["Evolutionary biology", "Evolutionary genetics", "genetics", "Genetic mutation", "population genetics", "microbiology", "Bacterial pathogens", "bacteriology", "Medical microbiology", "Infectious diseases", "Bacterial diseases", "Enterobacter infections", "insertion", "cassette", "elements", "non-transmissible", "plasmids", "mobilization"], "article_id"=>925397, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Thomas Guillard", "Antoine Grillon", "Christophe de Champs", "Céline Cartier", "Janick Madoux", "Béatrice Berçot", "Anne-Laure Lebreil", "Alain Lozniewski", "Jacques Riahi", "Véronique Vernet-Garnier", "Emmanuelle Cambau"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0087801.s001", "https://dx.doi.org/10.1371/journal.pone.0087801.s002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Mobile_Insertion_Cassette_Elements_Found_in_Small_Non_Transmissible_Plasmids_in_Proteeae_May_Explain_qnrD_Mobilization/925397", "title"=>"Mobile Insertion Cassette Elements Found in Small Non-Transmissible Plasmids in <i>Proteeae</i> May Explain <i>qnrD</i> Mobilization", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-02-04 03:26:59"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"21", "full-text"=>"24", "pdf"=>"14", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"2"}
  • {"unique-ip"=>"19", "full-text"=>"19", "pdf"=>"17", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"13", "full-text"=>"16", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"16", "full-text"=>"21", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"14", "full-text"=>"10", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"13", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"15", "full-text"=>"17", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"17", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"19", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"10", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"14", "full-text"=>"9", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"15", "full-text"=>"13", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"13", "full-text"=>"15", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"16", "full-text"=>"17", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"14", "full-text"=>"10", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"1", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"0", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"16", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"7", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"10", "full-text"=>"12", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"14", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"5", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"10"}
  • {"unique-ip"=>"6", "full-text"=>"1", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"12"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[282]}, {"subject_area"=>"/Biology and life sciences/Genetics", "average_usage"=>[306, 482]}, {"subject_area"=>"/Biology and life sciences/Microbiology", "average_usage"=>[317]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[292, 461]}, {"subject_area"=>"/Biology and life sciences/Organisms", "average_usage"=>[310]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[285]}, {"subject_area"=>"/Medicine and health sciences/Infectious diseases", "average_usage"=>[319]}, {"subject_area"=>"/Medicine and health sciences/Pathology and laboratory medicine", "average_usage"=>[287]}]}
Loading … Spinner
There are currently no alerts