Poly(Trimethylene Carbonate-co-ε-Caprolactone) Promotes Axonal Growth
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{"title"=>"Poly(trimethylene carbonate-co-ε-caprolactone) promotes axonal growth", "type"=>"journal", "authors"=>[{"first_name"=>"Daniela Nogueira", "last_name"=>"Rocha", "scopus_author_id"=>"56068112300"}, {"first_name"=>"Pedro", "last_name"=>"Brites", "scopus_author_id"=>"6506105762"}, {"first_name"=>"Carlos", "last_name"=>"Fonseca", "scopus_author_id"=>"55937370500"}, {"first_name"=>"Ana Paula", "last_name"=>"Pêgo", "scopus_author_id"=>"56053889300"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"issn"=>"19326203", "scopus"=>"2-s2.0-84896121626", "pui"=>"372609695", "doi"=>"10.1371/journal.pone.0088593", "sgr"=>"84896121626"}, "id"=>"fe629bb0-f72e-33a5-ac3d-6c005c843a01", "abstract"=>"Mammalian central nervous system (CNS) neurons do not regenerate after injury due to the inhibitory environment formed by the glial scar, largely constituted by myelin debris. The use of biomaterials to bridge the lesion area and the creation of an environment favoring axonal regeneration is an appealing approach, currently under investigation. This work aimed at assessing the suitability of three candidate polymers – poly(ε-caprolactone), poly(trimethylene carbonate-co-ε-caprolactone) (P(TMC-CL)) (11∶89 mol%) and poly(trimethylene carbonate) - with the final goal of using these materials in the development of conduits to promote spinal cord regeneration. Poly(L-lysine) (PLL) coated polymeric films were tested for neuronal cell adhesion and neurite outgrowth. At similar PLL film area coverage conditions, neuronal polarization and axonal elongation was significantly higher on P(TMC-CL) films. Furthermore, cortical neurons cultured on P(TMC-CL) were able to extend neurites even when seeded onto myelin. This effect was found to be mediated by the glycogen synthase kinase 3β (GSK3β) signaling pathway with impact on the collapsin response mediator protein 4 (CRMP4), suggesting that besides surface topography, nanomechanical properties were implicated in this process. The obtained results indicate P(TMC-CL) as a promising material for CNS regenerative applications as it promotes axonal growth, overcoming myelin inhibition.", "link"=>"http://www.mendeley.com/research/polytrimethylene-carbonateco%CE%B5caprolactone-promotes-axonal-growth", "reader_count"=>14, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Student > Doctoral Student"=>1, "Researcher"=>4, "Student > Ph. D. Student"=>3, "Student > Master"=>2, "Student > Bachelor"=>3}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Student > Doctoral Student"=>1, "Researcher"=>4, "Student > Ph. D. Student"=>3, "Student > Master"=>2, "Student > Bachelor"=>3}, "reader_count_by_subject_area"=>{"Engineering"=>4, "Unspecified"=>2, "Materials Science"=>2, "Medicine and Dentistry"=>1, "Agricultural and Biological Sciences"=>1, "Neuroscience"=>1, "Physics and Astronomy"=>2, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>4}, "Materials Science"=>{"Materials Science"=>2}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Neuroscience"=>{"Neuroscience"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>1}, "Unspecified"=>{"Unspecified"=>2}}, "reader_count_by_country"=>{"Canada"=>1}, "group_count"=>2}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1401245"], "description"=>"<p><b>A</b>. Fluorescently labeled cortical neurons, immunostained for TAU (green); nuclei are counterstainned with Hoechst (blue); <b>B.</b> Number of primary neurites per cell; <b>C.</b> Total neurite length; <b>D.</b> Average neurite length and <b>E.</b> Length of the longest neurite. (n = 130 cells, mean ± SD, *** for p<0.001).</p>", "links"=>[], "tags"=>["Biochemistry", "cytochemistry", "Extracellular matrix", "biotechnology", "Tissue engineering", "Molecular cell biology", "cell adhesion", "neuroscience", "Neurobiology of disease and regeneration", "biomaterials", "Material by structure", "polymers", "elastomers", "Material properties", "Mechanical properties", "nanotechnology", "neurology", "Demyelinating disorders", "biophysics", "biomechanics", "Tissue mechanics", "pll", "coated", "surfaces", "neurite", "elongation", "cellular"], "article_id"=>946801, "categories"=>["Physics", "Biological Sciences", "Medicine"], "users"=>["Daniela Nogueira Rocha", "Pedro Brites", "Carlos Fonseca", "Ana Paula Pêgo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0088593.g002", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_the_PLL_coated_surfaces_on_neurite_elongation_and_cellular_polarization_/946801", "title"=>"Effect of the PLL coated surfaces on neurite elongation and cellular polarization.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-27 03:15:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1401246"], "description"=>"<p><b>A</b>. Root mean square (RMS) roughness of all polymeric surfaces; <b>B</b>. Representative photographs of the polymeric surfaces before and after nanoindentation; images are color coded, showing elevated areas in bright and lower areas in dark color. <b>C</b>. Representative nanoindentation force-displacement curves; D. Mean hardness values of all polymeric surfaces, calculated for the maximum load and E. Mean stiffness values for all polymeric surfaces. (n = 60 indentations, mean ± SD, *** for p<0.001).</p>", "links"=>[], "tags"=>["Biochemistry", "cytochemistry", "Extracellular matrix", "biotechnology", "Tissue engineering", "Molecular cell biology", "cell adhesion", "neuroscience", "Neurobiology of disease and regeneration", "biomaterials", "Material by structure", "polymers", "elastomers", "Material properties", "Mechanical properties", "nanotechnology", "neurology", "Demyelinating disorders", "biophysics", "biomechanics", "Tissue mechanics", "tested", "polymeric"], "article_id"=>946802, "categories"=>["Physics", "Biological Sciences", "Medicine"], "users"=>["Daniela Nogueira Rocha", "Pedro Brites", "Carlos Fonseca", "Ana Paula Pêgo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0088593.g003", "stats"=>{"downloads"=>0, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Morphology_and_mechanical_properties_of_the_tested_polymeric_surfaces_/946802", "title"=>"Morphology and mechanical properties of the tested polymeric surfaces.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-27 03:15:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1401247"], "description"=>"<p><b>A.</b> Cortical neurons are immunostained for β-III tubulin (green) and nuclei are counterstainned with Hoechst (blue); myelin coating is immunostainned for MBP (green), surfaces were fully covered by myelin (see <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088593#pone.0088593.s002\" target=\"_blank\">fig. S2</a> for myelin quantification) <b>B.</b> Effect of myelin on the ability of neurons to extend processes is presented as the % of cells with neurites in relation to the total number of cells. (n = 3 independent studies, mean ± SD; ** for p<0.01).</p>", "links"=>[], "tags"=>["Biochemistry", "cytochemistry", "Extracellular matrix", "biotechnology", "Tissue engineering", "Molecular cell biology", "cell adhesion", "neuroscience", "Neurobiology of disease and regeneration", "biomaterials", "Material by structure", "polymers", "elastomers", "Material properties", "Mechanical properties", "nanotechnology", "neurology", "Demyelinating disorders", "biophysics", "biomechanics", "Tissue mechanics", "cns", "myelin", "neurite", "outgrowth", "cortical", "neurons", "cultured", "days", "substrates", "coated"], "article_id"=>946803, "categories"=>["Physics", "Biological Sciences", "Medicine"], "users"=>["Daniela Nogueira Rocha", "Pedro Brites", "Carlos Fonseca", "Ana Paula Pêgo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0088593.g004", "stats"=>{"downloads"=>1, "page_views"=>21, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_CNS_myelin_on_neurite_outgrowth_of_cortical_neurons_cultured_for_4_days_on_PLL_P_TMC_CL_substrates_coated_with_CNS_myelin_/946803", "title"=>"Effect of CNS myelin on neurite outgrowth of cortical neurons cultured for 4 days on PLL-P(TMC-CL) substrates coated with CNS myelin.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-27 03:15:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1401249"], "description"=>"<p><b>A.</b> Schematic representation of the different phosphorylation forms of GSK3β and their activity status; <b>B.</b> Analysis of the phosphorylated forms of GSK3β by western blot. Representative blots are shown. Expression levels of GSK3β isoforms, β1 and β2, are presented and quantified individually or together. (n = 3 independent studies, average ± SD); <b>C.</b> Morphology of neurons (immunostained for TAU in green and nuclei counterstained in blue) cultured for 24 hours in the presence of DMSO (control) or in the presence of 6-bromoindirubin-3′-acetoxime (BIO) at 30 and 300 nM. Quantifications of the longest neurite, average neurite length and the number of neurites per cell are shown (n = 130 cells, mean ± SD, * for p<0.05, ** for p<0.01 and *** for p<0.001); <b>D.</b> Determination of CRMP4 phosphorylation levels in cortical neurons plated for 4 days on control or P(TMC-CL). Representative western blot is shown and below the quantification (n = 3 independent studies, average ± SD).</p>", "links"=>[], "tags"=>["Biochemistry", "cytochemistry", "Extracellular matrix", "biotechnology", "Tissue engineering", "Molecular cell biology", "cell adhesion", "neuroscience", "Neurobiology of disease and regeneration", "biomaterials", "Material by structure", "polymers", "elastomers", "Material properties", "Mechanical properties", "nanotechnology", "neurology", "Demyelinating disorders", "biophysics", "biomechanics", "Tissue mechanics", "cortical", "neurons", "plated", "inhibition", "neurite"], "article_id"=>946805, "categories"=>["Physics", "Biological Sciences", "Medicine"], "users"=>["Daniela Nogueira Rocha", "Pedro Brites", "Carlos Fonseca", "Ana Paula Pêgo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0088593.g005", "stats"=>{"downloads"=>0, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Analysis_of_GSK3_946_in_cortical_neurons_plated_on_P_TMC_CL_and_effects_of_GSK3_946_inhibition_on_neurite_extension_/946805", "title"=>"Analysis of GSK3β in cortical neurons plated on P(TMC-CL) and effects of GSK3β inhibition on neurite extension.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-27 03:15:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1401250"], "description"=>"a)<p>Determined by 1H NMR on specimens purified by precipitation;</p>b)<p>Determined by GPC at 30°C using chloroform as the eluent.</p>", "links"=>[], "tags"=>["Biochemistry", "cytochemistry", "Extracellular matrix", "biotechnology", "Tissue engineering", "Molecular cell biology", "cell adhesion", "neuroscience", "Neurobiology of disease and regeneration", "biomaterials", "Material by structure", "polymers", "elastomers", "Material properties", "Mechanical properties", "nanotechnology", "neurology", "Demyelinating disorders", "biophysics", "biomechanics", "Tissue mechanics", "synthesized", "purified"], "article_id"=>946806, "categories"=>["Physics", "Biological Sciences", "Medicine"], "users"=>["Daniela Nogueira Rocha", "Pedro Brites", "Carlos Fonseca", "Ana Paula Pêgo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0088593.t001", "stats"=>{"downloads"=>3, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Characteristics_of_the_synthesized_and_purified_P_TMC_CL_co_polymers_/946806", "title"=>"Characteristics of the synthesized and purified P(TMC-CL) (co)polymers.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-02-27 03:15:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1401252", "https://ndownloader.figshare.com/files/1401253"], "description"=>"<div><p>Mammalian central nervous system (CNS) neurons do not regenerate after injury due to the inhibitory environment formed by the glial scar, largely constituted by myelin debris. The use of biomaterials to bridge the lesion area and the creation of an environment favoring axonal regeneration is an appealing approach, currently under investigation. This work aimed at assessing the suitability of three candidate polymers – poly(ε-caprolactone), poly(trimethylene carbonate-co-ε-caprolactone) (P(TMC-CL)) (11∶89 mol%) and poly(trimethylene carbonate) - with the final goal of using these materials in the development of conduits to promote spinal cord regeneration. Poly(L-lysine) (PLL) coated polymeric films were tested for neuronal cell adhesion and neurite outgrowth. At similar PLL film area coverage conditions, neuronal polarization and axonal elongation was significantly higher on P(TMC-CL) films. Furthermore, cortical neurons cultured on P(TMC-CL) were able to extend neurites even when seeded onto myelin. This effect was found to be mediated by the glycogen synthase kinase 3β (GSK3β) signaling pathway with impact on the collapsin response mediator protein 4 (CRMP4), suggesting that besides surface topography, nanomechanical properties were implicated in this process. The obtained results indicate P(TMC-CL) as a promising material for CNS regenerative applications as it promotes axonal growth, overcoming myelin inhibition.</p></div>", "links"=>[], "tags"=>["Biochemistry", "cytochemistry", "Extracellular matrix", "biotechnology", "Tissue engineering", "Molecular cell biology", "cell adhesion", "neuroscience", "Neurobiology of disease and regeneration", "biomaterials", "Material by structure", "polymers", "elastomers", "Material properties", "Mechanical properties", "nanotechnology", "neurology", "Demyelinating disorders", "biophysics", "biomechanics", "Tissue mechanics", "axonal"], "article_id"=>946808, "categories"=>["Physics", "Biological Sciences", "Medicine"], "users"=>["Daniela Nogueira Rocha", "Pedro Brites", "Carlos Fonseca", "Ana Paula Pêgo"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0088593.s001", "https://dx.doi.org/10.1371/journal.pone.0088593.s002"], "stats"=>{"downloads"=>2, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Poly_Trimethylene_Carbonate_co_949_Caprolactone_Promotes_Axonal_Growth_/946808", "title"=>"Poly(Trimethylene Carbonate-co-ε-Caprolactone) Promotes Axonal Growth", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-02-27 03:15:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1401243"], "description"=>"<p><b>A.</b> Number of cortical neurons with and without neurite extensions on polymeric surfaces coated with aqueous solutions at different concentrations of PLL. Glass coated with 24 µg.µl<sup>−1</sup> of PLL for 30 minutes was used as control. (n = 3 independent studies, mean ± SD, p<0.05) <b>B.</b> Percentage of PLL covered surface area as a function of the coating conditions. (n = 3, mean ± SD, p<0.05). x = condition not tested, 0 = null value. n.s. = non-significantly different from the control, α = total number of cells not significantly different from the control, β = number of cells with neurite extensions not significantly different from the control and χ = number of cells without extensions not significantly different from the control.</p>", "links"=>[], "tags"=>["Biochemistry", "cytochemistry", "Extracellular matrix", "biotechnology", "Tissue engineering", "Molecular cell biology", "cell adhesion", "neuroscience", "Neurobiology of disease and regeneration", "biomaterials", "Material by structure", "polymers", "elastomers", "Material properties", "Mechanical properties", "nanotechnology", "neurology", "Demyelinating disorders", "biophysics", "biomechanics", "Tissue mechanics", "neuron", "pll", "coated", "films", "respective", "homopolymers", "viable", "cells", "seeded"], "article_id"=>946799, "categories"=>["Physics", "Biological Sciences", "Medicine"], "users"=>["Daniela Nogueira Rocha", "Pedro Brites", "Carlos Fonseca", "Ana Paula Pêgo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0088593.g001", "stats"=>{"downloads"=>2, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Cortical_neuron_culture_on_PLL_coated_films_of_P_TMC_CL_and_respective_homopolymers_2_7_10_4_viable_cells_were_seeded_per_sample_/946799", "title"=>"Cortical neuron culture on PLL coated films of P(TMC-CL) and respective homopolymers (2.7×10<sup>4</sup> viable cells were seeded per sample).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-27 03:15:32"}

PMC Usage Stats | Further Information

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Relative Metric

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