Dermal Substitutes Support the Growth of Human Skin-Derived Mesenchymal Stromal Cells: Potential Tool for Skin Regeneration
Publication Date
February 26, 2014
Journal
PLOS ONE
Authors
Talita Da Silva Jeremias, Rafaela Grecco Machado, Silvia Beatriz Coutinho Visoni, Maurício José Pereima, et al
Volume
9
Issue
2
Pages
e89542
DOI
https://dx.plos.org/10.1371/journal.pone.0089542
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0089542
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/24586857
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3935879
Europe PMC
http://europepmc.org/abstract/MED/24586857
Web of Science
000332389000055
Scopus
84896272214
Mendeley
http://www.mendeley.com/research/dermal-substitutes-support-growth-human-skinderived-mesenchymal-stromal-cells-potential-tool-skin-re-1
Events
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Mendeley | Further Information

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Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1399213"], "description"=>"<p>(A) Morphological analysis of skin-derived cells by phase contrast microscopy. (B) MTS cell proliferation/viability assay. (C) Osteogenic and (D) adipogenic differentiation. (C) Cells cultured in inductive medium formed Alizarin Red S-stained mineralized nodules and (D) Oil red O-stained lipid clusters. (E) Flow cytometry analysis of hematopoietic (CD34, CD45) and MSC (CD90, CD73, CD105) markers. Specific markers are shown by black curves and controls by gray curves. ***p<0.001. Scale bar: (C–F): 50 µm. Other pictures: 200 µm.</p>", "links"=>[], "tags"=>["biotechnology", "Bioengineering", "Biomedical Engineering", "biomaterials", "Tissue engineering", "Molecular cell biology", "Cellular structures", "cytoskeleton", "Cellular types", "stem cells", "Adult stem cells", "Cell potency", "mesenchymal stem cells", "Extracellular space", "pericytes", "cell adhesion", "Cell growth", "Extracellular matrix", "phenotypic", "characterization", "skin-derived"], "article_id"=>945147, "categories"=>["Biological Sciences"], "users"=>["Talita da Silva Jeremias", "Rafaela Grecco Machado", "Silvia Beatriz Coutinho Visoni", "Maurício José Pereima", "Dilmar Francisco Leonardi", "Andréa Gonçalves Trentin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0089542.g001", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_MSC_phenotypic_characterization_of_human_skin_derived_cells_/945147", "title"=>"MSC phenotypic characterization of human skin-derived cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-26 03:30:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1399229"], "description"=>"<p>(A) Cross-sectional view of Integra dermal substitute alone showing the silicone (*) and the inner layer. (B–D) 3D culture of SD-MSCs in Integra 48 hours after seeding. (E) Surface and (F) cross-sectional views of Pelnac showing the collagen layer. (G–H) 3D culture of SD-MSCs in Pelnac 48 hours after seeding. Insets in B: different magnifications of a SD-MSC.</p>", "links"=>[], "tags"=>["biotechnology", "Bioengineering", "Biomedical Engineering", "biomaterials", "Tissue engineering", "Molecular cell biology", "Cellular structures", "cytoskeleton", "Cellular types", "stem cells", "Adult stem cells", "Cell potency", "mesenchymal stem cells", "Extracellular space", "pericytes", "cell adhesion", "Cell growth", "Extracellular matrix", "electron", "microscopy", "images", "sd-mscs", "cultured", "integra"], "article_id"=>945160, "categories"=>["Biological Sciences"], "users"=>["Talita da Silva Jeremias", "Rafaela Grecco Machado", "Silvia Beatriz Coutinho Visoni", "Maurício José Pereima", "Dilmar Francisco Leonardi", "Andréa Gonçalves Trentin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0089542.g004", "stats"=>{"downloads"=>4, "page_views"=>26, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Scanning_electron_microscopy_SEM_images_of_SD_MSCs_cultured_in_A_8211_D_Integra_and_E_8211_H_Pelnac_/945160", "title"=>"Scanning electron microscopy (SEM) images of SD-MSCs cultured in (A–D) Integra and (E–H) Pelnac.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-26 03:30:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1399238", "https://ndownloader.figshare.com/files/1399239"], "description"=>"<div><p>New strategies for skin regeneration are needed in order to provide effective treatment for cutaneous wounds and disease. Mesenchymal stem cells (MSCs) are an attractive source of cells for tissue engineering because of their prolonged self-renewal capacity, multipotentiality, and ability to release active molecules important for tissue repair. In this paper, we show that human skin-derived mesenchymal stromal cells (SD-MSCs) display similar characteristics to the multipotent MSCs. We also evaluate their growth in a three-dimensional (3D) culture system with dermal substitutes (Integra and Pelnac). When cultured in monolayers, SD-MSCs expressed mesenchymal markers, such as CD105, Fibronectin, and α-SMA; and neural markers, such as Nestin and βIII-Tubulin; at transcriptional and/or protein level. Integra and Pelnac equally supported the adhesion, spread and growth of human SD-MSCs in 3D culture, maintaining the MSC characteristics and the expression of multilineage markers. Therefore, dermal substitutes support the growth of mesenchymal stromal cells from human skin, promising an effective tool for tissue engineering and regenerative technology.</p></div>", "links"=>[], "tags"=>["biotechnology", "Bioengineering", "Biomedical Engineering", "biomaterials", "Tissue engineering", "Molecular cell biology", "Cellular structures", "cytoskeleton", "Cellular types", "stem cells", "Adult stem cells", "Cell potency", "mesenchymal stem cells", "Extracellular space", "pericytes", "cell adhesion", "Cell growth", "Extracellular matrix", "substitutes", "skin-derived", "mesenchymal", "stromal"], "article_id"=>945169, "categories"=>["Biological Sciences"], "users"=>["Talita da Silva Jeremias", "Rafaela Grecco Machado", "Silvia Beatriz Coutinho Visoni", "Maurício José Pereima", "Dilmar Francisco Leonardi", "Andréa Gonçalves Trentin"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0089542.s001", "https://dx.doi.org/10.1371/journal.pone.0089542.s002"], "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Dermal_Substitutes_Support_the_Growth_of_Human_Skin_Derived_Mesenchymal_Stromal_Cells_Potential_Tool_for_Skin_Regeneration_/945169", "title"=>"Dermal Substitutes Support the Growth of Human Skin-Derived Mesenchymal Stromal Cells: Potential Tool for Skin Regeneration", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-02-26 03:30:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1399220"], "description"=>"<p>(A) Gene expression profile by RT-PCR of SD-MSCs cultivated in standard medium. (B–E) Immunofluorescence staining of (B) CD105 and (C) Fibronectin. (D–F) Double-staining of α-SMA and βIII-Tubulin, (G–I) α-SMA and Nestin and (J–L) βIII-Tubulin and Nestin co-expression. (F, I and L): Merged pictures of D–E, G–H, J–K, respectively. Cell nuclei were stained with DAPI (blue). Arrows: Nestin nuclear staining. Scale bar: 50 µm.</p>", "links"=>[], "tags"=>["biotechnology", "Bioengineering", "Biomedical Engineering", "biomaterials", "Tissue engineering", "Molecular cell biology", "Cellular structures", "cytoskeleton", "Cellular types", "stem cells", "Adult stem cells", "Cell potency", "mesenchymal stem cells", "Extracellular space", "pericytes", "cell adhesion", "Cell growth", "Extracellular matrix"], "article_id"=>945154, "categories"=>["Biological Sciences"], "users"=>["Talita da Silva Jeremias", "Rafaela Grecco Machado", "Silvia Beatriz Coutinho Visoni", "Maurício José Pereima", "Dilmar Francisco Leonardi", "Andréa Gonçalves Trentin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0089542.g002", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Multilineage_potential_of_SD_MSCs_/945154", "title"=>"Multilineage potential of SD-MSCs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-26 03:30:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1399236"], "description"=>"<p>RT-PCR analysis showing mRNA expression of Nestin, βIII-Tubulin, α-SMA, CD31 and GAPDH (as internal control) in SD-MSCs cultured in dermal substitutes. Control: SD-MSCs cultured on plastic surfaces.</p>", "links"=>[], "tags"=>["biotechnology", "Bioengineering", "Biomedical Engineering", "biomaterials", "Tissue engineering", "Molecular cell biology", "Cellular structures", "cytoskeleton", "Cellular types", "stem cells", "Adult stem cells", "Cell potency", "mesenchymal stem cells", "Extracellular space", "pericytes", "cell adhesion", "Cell growth", "Extracellular matrix", "sd-mscs", "cultured", "integra"], "article_id"=>945167, "categories"=>["Biological Sciences"], "users"=>["Talita da Silva Jeremias", "Rafaela Grecco Machado", "Silvia Beatriz Coutinho Visoni", "Maurício José Pereima", "Dilmar Francisco Leonardi", "Andréa Gonçalves Trentin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0089542.g006", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Gene_expression_profile_of_human_SD_MSCs_cultured_in_Integra_and_Pelnac_/945167", "title"=>"Gene expression profile of human SD-MSCs cultured in Integra and Pelnac.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-26 03:30:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1399235"], "description"=>"<p>Flow cytometry analysis of SD-MSCs (CD90, CD73, CD105) and hematopoietic (CD45) markers in SD-MSCs after 3 days of culture in Integra (upper panel) and Pelnac (lower panel). Curves in black show the specific markers, and gray curves correspond to controls.</p>", "links"=>[], "tags"=>["biotechnology", "Bioengineering", "Biomedical Engineering", "biomaterials", "Tissue engineering", "Molecular cell biology", "Cellular structures", "cytoskeleton", "Cellular types", "stem cells", "Adult stem cells", "Cell potency", "mesenchymal stem cells", "Extracellular space", "pericytes", "cell adhesion", "Cell growth", "Extracellular matrix", "sd-mscs", "cultured", "integra"], "article_id"=>945166, "categories"=>["Biological Sciences"], "users"=>["Talita da Silva Jeremias", "Rafaela Grecco Machado", "Silvia Beatriz Coutinho Visoni", "Maurício José Pereima", "Dilmar Francisco Leonardi", "Andréa Gonçalves Trentin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0089542.g005", "stats"=>{"downloads"=>2, "page_views"=>25, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Immunophenotypic_profile_of_human_SD_MSCs_cultured_in_Integra_and_Pelnac_/945166", "title"=>"Immunophenotypic profile of human SD-MSCs cultured in Integra and Pelnac.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-26 03:30:32"}
  • {"files"=>["https://ndownloader.figshare.com/files/1399226"], "description"=>"<p>Confocal microscopy of SD-MSCs cultured in (A–C) Integra and (D–F) Pelnac. (A and E) DAPI nuclear staining of SD-MSCs. (B and F) Integra and (F) Pelnac dermal substitutes, respectively (green autofluorescence). (C and G) merged images of (A and B) and (E and F), respectively. (D and H) MTS cell viability assay of SD-MSCs cultivated in Integra and Pelnac, respectively. ***p<0.001, **p<0.01. Scale bar: 100 um.</p>", "links"=>[], "tags"=>["biotechnology", "Bioengineering", "Biomedical Engineering", "biomaterials", "Tissue engineering", "Molecular cell biology", "Cellular structures", "cytoskeleton", "Cellular types", "stem cells", "Adult stem cells", "Cell potency", "mesenchymal stem cells", "Extracellular space", "pericytes", "cell adhesion", "Cell growth", "Extracellular matrix", "cultures", "sd-mscs", "integra"], "article_id"=>945157, "categories"=>["Biological Sciences"], "users"=>["Talita da Silva Jeremias", "Rafaela Grecco Machado", "Silvia Beatriz Coutinho Visoni", "Maurício José Pereima", "Dilmar Francisco Leonardi", "Andréa Gonçalves Trentin"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0089542.g003", "stats"=>{"downloads"=>1, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_3D_cultures_of_human_SD_MSCs_in_A_8211_D_Integra_and_E_8211_H_Pelnac_/945157", "title"=>"3D cultures of human SD-MSCs in (A–D) Integra and (E–H) Pelnac.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-26 03:30:32"}

PMC Usage Stats | Further Information

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Relative Metric

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