Quantitative Field Testing Heterodera glycines from Metagenomic DNA Samples Isolated Directly from Soil under Agronomic Production
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{"title"=>"Quantitative field testing Heterodera glycines from metagenomic DNA samples isolated directly from soil under agronomic production", "type"=>"journal", "authors"=>[{"first_name"=>"Yan", "last_name"=>"Li", "scopus_author_id"=>"57192523101"}, {"first_name"=>"Gary W.", "last_name"=>"Lawrence", "scopus_author_id"=>"7202287746"}, {"first_name"=>"Shien", "last_name"=>"Lu", "scopus_author_id"=>"7404228825"}, {"first_name"=>"Clarissa", "last_name"=>"Balbalian", "scopus_author_id"=>"54683454600"}, {"first_name"=>"Vincent P.", "last_name"=>"Klink", "scopus_author_id"=>"9332962700"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"24587100", "scopus"=>"2-s2.0-84896077837", "sgr"=>"84896077837", "doi"=>"10.1371/journal.pone.0089887", "pui"=>"372599111", "issn"=>"19326203", "isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)"}, "id"=>"c7dfb281-dac9-3268-bba3-0c15649906a0", "abstract"=>"A quantitative PCR procedure targeting the Heterodera glycines ortholog of the Caenorhabditis elegans uncoordinated-78 gene was developed. The procedure estimated the quantity of H. glycines from metagenomic DNA samples isolated directly from field soil under agronomic production. The estimation of H. glycines quantity was determined in soil samples having other soil dwelling plant parasitic nematodes including Hoplolaimus, predatory nematodes including Mononchus, free-living nematodes and biomass. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from field soil.", "link"=>"http://www.mendeley.com/research/quantitative-field-testing-heterodera-glycines-metagenomic-dna-samples-isolated-directly-soil-under", "reader_count"=>18, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Librarian"=>1, "Student > Doctoral Student"=>1, "Researcher"=>7, "Student > Postgraduate"=>3, "Student > Master"=>3, "Student > Bachelor"=>1, "Lecturer"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Librarian"=>1, "Student > Doctoral Student"=>1, "Researcher"=>7, "Student > Postgraduate"=>3, "Student > Master"=>3, "Student > Bachelor"=>1, "Lecturer"=>1}, "reader_count_by_subject_area"=>{"Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>3, "Agricultural and Biological Sciences"=>9, "Medicine and Dentistry"=>2, "Chemistry"=>1, "Social Sciences"=>2}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>2}, "Chemistry"=>{"Chemistry"=>1}, "Social Sciences"=>{"Social Sciences"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>9}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}}, "reader_count_by_country"=>{"United States"=>1}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1396816"], "description"=>"<p>Lane 1, Hg-unc 9; Lane 2, Hg-unc 22; Lane 3, Hg-unc 31; Lane 4, Hg-unc 52; Lane 5, Hg-unc 115; Lane 6, Hg-unc 101; Lane 7, Hg-dys-1; Lane 8, Hg-nep-1; Lane 9, Hg-unc 89; Lane 10, Hg-unc 78; Lane 11, Hg-MRCK-1.</p>", "links"=>[], "tags"=>["Agricultural biotechnology", "Agricultural production", "Environmental impacts", "Agroecology", "Agronomic ecology", "crops", "Crop diseases", "Crop management", "Pest control", "Soil science", "Computational biology", "genomics", "metagenomics", "ecology", "Soil ecology", "Model organisms", "Animal models", "Caenorhabditis elegans", "hg-unc", "primer"], "article_id"=>943151, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Yan Li", "Gary W. Lawrence", "Shien Lu", "Clarissa Balbalian", "Vincent P. Klink"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0089887.g001", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Screening_of_Hg_unc_primer_pairs_/943151", "title"=>"Screening of Hg-unc primer pairs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-24 03:26:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/1396817"], "description"=>"<p>Lanes 1–3, 1 SCN; Lanes 4–6, 10 SCN; Lanes 7–9, 100 SCN; Lanes 10–12, 1,000 SCN.</p>", "links"=>[], "tags"=>["Agricultural biotechnology", "Agricultural production", "Environmental impacts", "Agroecology", "Agronomic ecology", "crops", "Crop diseases", "Crop management", "Pest control", "Soil science", "Computational biology", "genomics", "metagenomics", "ecology", "Soil ecology", "Model organisms", "Animal models", "Caenorhabditis elegans", "amplification", "hg-unc-78", "numbers", "extracted", "j2", "cultured"], "article_id"=>943152, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Yan Li", "Gary W. Lawrence", "Shien Lu", "Clarissa Balbalian", "Vincent P. Klink"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0089887.g002", "stats"=>{"downloads"=>2, "page_views"=>27, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_DNA_amplification_of_Hg_unc_78_from_different_numbers_of_pure_extracted_H_glycines_SCN_J2_cultured_in_soil_in_greenhouse_/943152", "title"=>"DNA amplification of Hg-unc-78 from different numbers of pure, extracted <i>H. glycines</i> (SCN) J2 cultured in soil in greenhouse.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-24 03:26:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/1396818"], "description"=>"<p>Lanes 1–3, 1 SCN; Lanes 4–6, 10 SCN; Lanes 7–9, 100 SCN; Lanes 10–12, 1,000 SCN; Lane 13, without primer (control); Lane 14, without DNA (control).</p>", "links"=>[], "tags"=>["Agricultural biotechnology", "Agricultural production", "Environmental impacts", "Agroecology", "Agronomic ecology", "crops", "Crop diseases", "Crop management", "Pest control", "Soil science", "Computational biology", "genomics", "metagenomics", "ecology", "Soil ecology", "Model organisms", "Animal models", "Caenorhabditis elegans", "amplification", "hg-unc-78", "metagenomic", "dna", "containing", "numbers"], "article_id"=>943153, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Yan Li", "Gary W. Lawrence", "Shien Lu", "Clarissa Balbalian", "Vincent P. Klink"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0089887.g003", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_DNA_amplification_of_Hg_unc_78_from_metagenomic_DNA_isolated_directly_from_soil_containing_different_numbers_of_H_glycines_SCN_/943153", "title"=>"DNA amplification of Hg-unc-78 from metagenomic DNA isolated directly from soil containing different numbers of <i>H. glycines</i> (SCN).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-24 03:26:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/1396819"], "description"=>"<p>Lanes 1–3; 100 Rr; Lanes 4–6, 1,000 Rr; Lanes 7–9, 100 RKN; Lanes 10–12, 1,000 RKN; Lanes 13–15, 100 SCN; Lanes 16–18, 1,000 SCN; Lane 19, without primers (control); Lane 20, without DNA (control). Rr, <i>Rotylenchulus reniformis</i>; RKN, <i>Meloidogyne incognita</i>.</p>", "links"=>[], "tags"=>["Agricultural biotechnology", "Agricultural production", "Environmental impacts", "Agroecology", "Agronomic ecology", "crops", "Crop diseases", "Crop management", "Pest control", "Soil science", "Computational biology", "genomics", "metagenomics", "ecology", "Soil ecology", "Model organisms", "Animal models", "Caenorhabditis elegans", "specificity", "hg-unc78", "pcr", "dna", "numbers", "extracted", "off-target", "nematodes", "compared"], "article_id"=>943154, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Yan Li", "Gary W. Lawrence", "Shien Lu", "Clarissa Balbalian", "Vincent P. Klink"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0089887.g004", "stats"=>{"downloads"=>0, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_specificity_of_the_Hg_unc78_PCR_reaction_from_DNA_isolated_from_different_numbers_of_pure_extracted_off_target_nematodes_as_compared_to_DNA_isolated_from_pure_extracted_H_glycines_/943154", "title"=>"The specificity of the Hg-unc78 PCR reaction from DNA isolated from different numbers of pure, extracted off-target nematodes as compared to DNA isolated from pure, extracted <i>H. glycines</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-24 03:26:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/1396820"], "description"=>"<p>Lanes 1–3, metagenomic DNA including 1 SCN J2; Lanes 4–6, metagenomic DNA including 10 SCN J2; Lanes 7–9, metagenomic DNA including 100 SCN J2; Lanes 10–12, metagenomic DNA including 1,000 SCN J2; Lane 13, cloned gene without primers (control); Lane 14, without DNA (control); Lane 15, positive control, amplification product is Hg-unc 78 amplified from the cloned gene.</p>", "links"=>[], "tags"=>["Agricultural biotechnology", "Agricultural production", "Environmental impacts", "Agroecology", "Agronomic ecology", "crops", "Crop diseases", "Crop management", "Pest control", "Soil science", "Computational biology", "genomics", "metagenomics", "ecology", "Soil ecology", "Model organisms", "Animal models", "Caenorhabditis elegans", "amplification", "hg-unc78", "metagenomic", "dna"], "article_id"=>943155, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Yan Li", "Gary W. Lawrence", "Shien Lu", "Clarissa Balbalian", "Vincent P. Klink"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0089887.g005", "stats"=>{"downloads"=>0, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_DNA_amplification_of_the_Hg_unc78_from_metagenomic_DNA_samples_/943155", "title"=>"DNA amplification of the Hg-unc78 from metagenomic DNA samples.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-24 03:26:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/1396821"], "description"=>"<p>Standard curve obtained using pure greenhouse samples, (b) soil samples. The statistical significance between the actual number of SCN and the estimated number are provided.</p>", "links"=>[], "tags"=>["Agricultural biotechnology", "Agricultural production", "Environmental impacts", "Agroecology", "Agronomic ecology", "crops", "Crop diseases", "Crop management", "Pest control", "Soil science", "Computational biology", "genomics", "metagenomics", "ecology", "Soil ecology", "Model organisms", "Animal models", "Caenorhabditis elegans"], "article_id"=>943156, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Yan Li", "Gary W. Lawrence", "Shien Lu", "Clarissa Balbalian", "Vincent P. Klink"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0089887.g006", "stats"=>{"downloads"=>0, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Estimation_of_H_glycines_population_size_by_qPCR_/943156", "title"=>"Estimation of <i>H. glycines</i> population size by qPCR.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-24 03:26:02"}
  • {"files"=>["https://ndownloader.figshare.com/files/1396822"], "description"=>"<p>Standard curve obtained from metagenomic DNA isolated directly from soil samples. The statistical significance between the actual number of SCN and the estimated number are provided.</p>", "links"=>[], "tags"=>["Agricultural biotechnology", "Agricultural production", "Environmental impacts", "Agroecology", "Agronomic ecology", "crops", "Crop diseases", "Crop management", "Pest control", "Soil science", "Computational biology", "genomics", "metagenomics", "ecology", "Soil ecology", "Model organisms", "Animal models", "Caenorhabditis elegans"], "article_id"=>943157, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Yan Li", "Gary W. Lawrence", "Shien Lu", "Clarissa Balbalian", "Vincent P. Klink"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0089887.g007", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Estimation_of_H_glycines_population_size_by_qPCR_/943157", "title"=>"Estimation of <i>H. glycines</i> population size by qPCR.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-24 03:26:02"}
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PMC Usage Stats | Further Information

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Relative Metric

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