Cardiac Function and Architecture Are Maintained in a Model of Cardiorestricted Overexpression of the Prorenin-Renin Receptor
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Cardiac function and architecture are maintained in a model of cardiorestricted overexpression of the prorenin-renin receptor", "type"=>"journal", "authors"=>[{"first_name"=>"Hasan", "last_name"=>"Mahmud", "scopus_author_id"=>"23091445900"}, {"first_name"=>"Wellington Mardoqueu", "last_name"=>"Candido", "scopus_author_id"=>"56067866200"}, {"first_name"=>"Linda", "last_name"=>"Van Genne", "scopus_author_id"=>"6507333385"}, {"first_name"=>"Inge", "last_name"=>"Vreeswijk-Baudoin", "scopus_author_id"=>"36515892600"}, {"first_name"=>"Hongjuan", "last_name"=>"Yu", "scopus_author_id"=>"55184634500"}, {"first_name"=>"Bart", "last_name"=>"Van De Sluis", "scopus_author_id"=>"6602558146"}, {"first_name"=>"Jan", "last_name"=>"Van Deursen", "scopus_author_id"=>"7003980379"}, {"first_name"=>"Wiek H.", "last_name"=>"Van Gilst", "scopus_author_id"=>"7103238396"}, {"first_name"=>"Herman H.W.", "last_name"=>"Silljé", "scopus_author_id"=>"6602526370"}, {"first_name"=>"Rudolf A.", "last_name"=>"De Boer", "scopus_author_id"=>"8572907800"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pmid"=>"24587131", "sgr"=>"84896110467", "arxiv"=>"artigo 3", "doi"=>"10.1371/journal.pone.0089929", "scopus"=>"2-s2.0-84896110467", "pui"=>"372609133", "issn"=>"19326203"}, "id"=>"a976064a-cd6b-3efd-9f82-5f7e043d7b56", "abstract"=>"The (pro)renin-renin receptor, (P)RR has been claimed to be a novel element of the renin-angiotensin system (RAS). The function of (P)RR has been widely studied in renal and vascular pathology but the cardio-specific function of (P)RR has not been studied in detail. We therefore generated a transgenic mouse (Tg) with cardio-restricted (P)RR overexpression driven by the alpha-MHC promotor. The mRNA expression of (P)RR was ∼ 170-fold higher (P<0.001) and protein expression ∼ 5-fold higher (P<0.001) in hearts of Tg mice as compared to non-transgenic (wild type, Wt) littermates. This level of overexpression was not associated with spontaneous cardiac morphological or functional abnormalities in Tg mice. To assess whether (P)RR could play a role in cardiac hypertrophy, we infused ISO for 28 days, but this caused an equal degree of cardiac hypertrophy and fibrosis in Wt and Tg mice. In addition, ischemia-reperfusion injury was performed in Langendorff perfused isolated mouse hearts. We did not observe differences in parameters of cardiac function or damage between Wt and Tg mouse hearts under these conditions. Finally, we explored whether the hypoxia sensing response would be modulated by (P)RR using HeLa cells with and without (P)RR overexpression. We did not establish any effect of (P)RR on expression of genes associated with the hypoxic response. These results demonstrate that cardio-specific overexpression of (P)RR does not provoke phenotypical differences in the heart, and does not affect the hearts' response to stress and injury. It is concluded that increased myocardial (P)RR expression is unlikely to have a major role in pathological cardiac remodeling.", "link"=>"http://www.mendeley.com/research/cardiac-function-architecture-maintained-model-cardiorestricted-overexpression-proreninrenin-recepto", "reader_count"=>8, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Student > Ph. D. Student"=>2, "Student > Master"=>2, "Student > Bachelor"=>1, "Professor"=>2}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Student > Ph. D. Student"=>2, "Student > Master"=>2, "Student > Bachelor"=>1, "Professor"=>2}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>1, "Medicine and Dentistry"=>4, "Agricultural and Biological Sciences"=>2, "Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>4}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>2}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"Brazil"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84896110467"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84896110467?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84896110467&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84896110467&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84896110467", "dc:identifier"=>"SCOPUS_ID:84896110467", "eid"=>"2-s2.0-84896110467", "dc:title"=>"Cardiac function and architecture are maintained in a model of cardiorestricted overexpression of the prorenin-renin receptor", "dc:creator"=>"Mahmud H.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"9", "prism:issueIdentifier"=>"2", "prism:pageRange"=>nil, "prism:coverDate"=>"2014-02-25", "prism:coverDisplayDate"=>"25 February 2014", "prism:doi"=>"10.1371/journal.pone.0089929", "citedby-count"=>"7", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"University of Groningen, University Medical Center Groningen", "affiliation-city"=>"Groningen", "affiliation-country"=>"Netherlands"}], "pubmed-id"=>"24587131", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e89929", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0089929", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"2", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"6", "html_views"=>"21"}
  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"39", "xml_views"=>"3", "html_views"=>"106"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"3", "html_views"=>"64"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"43"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"34"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"6", "xml_views"=>"2", "html_views"=>"45"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"21"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"4", "html_views"=>"25"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"26"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"22"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"2", "html_views"=>"23"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"2", "html_views"=>"29"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"55"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"25"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"3", "html_views"=>"4"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"4", "html_views"=>"13"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"2"}

Figshare

  • {"files"=>["https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/1398097/Figure_1.tif"], "description"=>"<p>A) Schematic depiction of the generation of Tg mice with cardiomyocyte-restricted (P)RR overexpression. We cloned the 1060 bp murine (P)RR cDNA in front of the a 5.5-kb α-MHC promoter. B) (P)RR mRNA expression normalized to 36B4 by RT-PCR of Tg mice and their Wt littermates (left upper panel); western blot for (P)RR and GAPDH protein from hearts of Tg and Wt mice (bottom panel) and quantification of the western blot (right upper panel). C) Heart-weight (HW) normalized to body weight (BW) in adult Wt and Tg mice (left upper panel); images for Masson staining for quantification of fibrosis (bar size 100 µm) and haematoxylin and Eosin staining (bar size 70 µm) for assessment of cardiac morphology (bottom pannels) and quantification of Masson staining (right upper panel). D) mRNA expression of <i>ANP</i>, <i>BNP</i>, <i>Gal-3</i>, <i>Col-1</i>, <i>Col-3,</i> and <i>MCIP1</i> (normalized to <i>36B4</i>) of the Wt and Tg mice. Fold changes are shown. *** <i>P<0.001</i>, Wt vs. Tg.</p>", "links"=>[], "tags"=>["Anatomy and physiology", "cardiovascular system", "biotechnology", "Genetic engineering", "transgenics", "Model organisms", "Animal models", "mouse", "cardiovascular", "Heart failure", "hypertension", "transgenic", "mice", "baseline"], "article_id"=>944238, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Hasan Mahmud", "Wellington Mardoqueu Candido", "Linda van Genne", "Inge Vreeswijk-Baudoin", "Hongjuan Yu", "Bart van de Sluis", "Jan van Deursen", "Wiek H. van Gilst", "Herman H. W. Silljé", "Rudolf A. de Boer"], "doi"=>["http://dx.doi.org/10.1371/journal.pone.0089929.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"http://figshare.com/articles/_Generation_of_P_RR_transgenic_mice_and_baseline_characteristics_/944238", "title"=>"Generation of (P)RR transgenic mice and baseline characteristics.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-25 03:22:48"}
  • {"files"=>["https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/1398107/Figure_2.tif"], "description"=>"<p>A) Left-ventricular weight (LV-W) to tibia length (TL)in adult Wt and Tg mice subjected to saline or ISO infusion for 28 days (N = 7 mice in each group). B) <i>ANP</i> mRNA expression (normalized to <i>36B4</i>) in adult Wt and Tg mice subjected to saline or ISO infusion for 28 days (N = 7 mice in each group). C) Renin mRNA expression (normalized to <i>36B4</i>) in adult Wt and Tg mice subjected to saline or ISO infusion for 28 days (N = 7 mice in each group). D) <i>α-MHC/β-MHC</i> (ratio) mRNA expression (normalized to <i>36B4</i>) in adult Wt and Tg mice subjected to saline or ISO infusion for 28 days (N = 7 mice in each group). E and F) Masson’s trichrome staining (bar size 100 µm) to assess myocardial fibrosis in adult Wt and Tg mice subjected to saline (sham procedure) or ISO infusion for 28 days; E: quantification, and F: typical examples of Masson’s trichrome staining. G and H) FITC-WGA staining to assess myocyte hypertrophy in adult Wt and Tg mice subjected to saline (sham procedure) or ISO infusion for 28 days; G: quantification, and H: typical examples of FITC-WGA staining. *<i>P</i><0.05, **<i>P</i><0.01, sham vs. ISO between Wt mice; <sup>#</sup><i>P</i><0.05, <sup>##</sup><i>P</i><0.01, sham vs. ISO between Tg mice and <sup>§</sup><i>P</i><0.05, ISO treatment between Wt and Tg mice.</p>", "links"=>[], "tags"=>["Anatomy and physiology", "cardiovascular system", "biotechnology", "Genetic engineering", "transgenics", "Model organisms", "Animal models", "mouse", "cardiovascular", "Heart failure", "hypertension", "isoproterenol", "induced", "cardiac", "hypertrophy", "transgenic"], "article_id"=>944241, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Hasan Mahmud", "Wellington Mardoqueu Candido", "Linda van Genne", "Inge Vreeswijk-Baudoin", "Hongjuan Yu", "Bart van de Sluis", "Jan van Deursen", "Wiek H. van Gilst", "Herman H. W. Silljé", "Rudolf A. de Boer"], "doi"=>["http://dx.doi.org/10.1371/journal.pone.0089929.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"http://figshare.com/articles/_Assessment_of_isoproterenol_induced_cardiac_hypertrophy_in_P_RR_transgenic_mice_/944241", "title"=>"Assessment of isoproterenol induced cardiac hypertrophy in (P)RR transgenic mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-25 03:22:48"}
  • {"files"=>["https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/1398108/Figure_3.tif"], "description"=>"<p><b>A)</b> Western blot of the expression of ERK1/2, p-38 and HSP47 proteins from hearts of Tg and Wt mice with and without ISO treatment. B) Quantification of the ratio of phospho−/total Erk1/2 from hearts of Tg and Wt mice with and without ISO treatment. C) Quantification of the ratio of phospho−/total p-38 from hearts of Tg and Wt mice with and without ISO treatment. D) Quantification of the HSP47 protein expression from hearts of Tg and Wt mice with and without ISO treatment. * <i>P<0.05</i>, Wt vs. Tg.</p>", "links"=>[], "tags"=>["Anatomy and physiology", "cardiovascular system", "biotechnology", "Genetic engineering", "transgenics", "Model organisms", "Animal models", "mouse", "cardiovascular", "Heart failure", "hypertension", "blot", "p-38"], "article_id"=>944242, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Hasan Mahmud", "Wellington Mardoqueu Candido", "Linda van Genne", "Inge Vreeswijk-Baudoin", "Hongjuan Yu", "Bart van de Sluis", "Jan van Deursen", "Wiek H. van Gilst", "Herman H. W. Silljé", "Rudolf A. de Boer"], "doi"=>["http://dx.doi.org/10.1371/journal.pone.0089929.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"http://figshare.com/articles/_Western_blot_of_ERK1_2_p_38_and_HSP47_/944242", "title"=>"Western blot of ERK1/2, p-38 and HSP47.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-25 03:22:48"}
  • {"files"=>["https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/1398110/Figure_4.tif"], "description"=>"<p>A) Leakage of lactate dehydrogenase (LDH) before and after ischemia and 5, 10 and 30 min after reperfusion of Wt and Tg mice. No significant difference was found between Wt and Tg mice. B) Leakage of creatine kinase (CK) before and after ischemia and 5, 10 and 30 min after reperfusion of Wt and Tg mice. No significant difference was found between Wt and Tg mice. C) Functional recovery; LV developed pressure (LVDP) for Wt and (P)RR Tg hearts after 30 min of reperfusion. D) Rate–pressure product (RPP) for Wt and (P)RR Tg hearts after 30 min of reperfusion. Values are means ± SEM; n = 6 per group.</p>", "links"=>[], "tags"=>["Anatomy and physiology", "cardiovascular system", "biotechnology", "Genetic engineering", "transgenics", "Model organisms", "Animal models", "mouse", "cardiovascular", "Heart failure", "hypertension", "ex", "vivo", "ischemia", "reperfusion", "langendorff", "perfusion", "tg"], "article_id"=>944244, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Hasan Mahmud", "Wellington Mardoqueu Candido", "Linda van Genne", "Inge Vreeswijk-Baudoin", "Hongjuan Yu", "Bart van de Sluis", "Jan van Deursen", "Wiek H. van Gilst", "Herman H. W. Silljé", "Rudolf A. de Boer"], "doi"=>["http://dx.doi.org/10.1371/journal.pone.0089929.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"http://figshare.com/articles/_Assessment_of_ex_vivo_ischemia_reperfusion_injury_by_Langendorff_isolated_heart_perfusion_in_P_RR_Tg_mice_/944244", "title"=>"Assessment of ex vivo ischemia reperfusion injury by Langendorff isolated heart perfusion in (P)RR Tg mice.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-25 03:22:48"}
  • {"files"=>["https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/1398111/Figure_5.tif"], "description"=>"<p>Gene expression levels in HeLa cells under normoxia, DFO (deferoxamine) treatment and hypoxia; HeLa cells without (fine bars) and with (black bars) (P)RR overexpression. A) <i>ADM</i> B) <i>VEGF</i> C) <i>c-jun</i> D) <i>c-fos</i> (all normalized to <i>GAPDH</i>). *<i>P<0.05</i>, ***<i>P<0.001</i>, normoxia vs. DFO or normoxia vs. hypoxia, under control condition. <sup>#</sup><i>P<0.05, </i><sup>##</sup><i>P<0.01</i> and <sup>###</sup><i>P<0.001</i>, normoxia vs. DFO or normoxia vs. hypoxia, under (P)RR overexpression.</p>", "links"=>[], "tags"=>["Anatomy and physiology", "cardiovascular system", "biotechnology", "Genetic engineering", "transgenics", "Model organisms", "Animal models", "mouse", "cardiovascular", "Heart failure", "hypertension", "overexpression"], "article_id"=>944245, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Hasan Mahmud", "Wellington Mardoqueu Candido", "Linda van Genne", "Inge Vreeswijk-Baudoin", "Hongjuan Yu", "Bart van de Sluis", "Jan van Deursen", "Wiek H. van Gilst", "Herman H. W. Silljé", "Rudolf A. de Boer"], "doi"=>["http://dx.doi.org/10.1371/journal.pone.0089929.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"http://figshare.com/articles/_Effect_of_P_RR_overexpression_on_stress_related_gene_expression_due_to_hypoxia_/944245", "title"=>"Effect of (P)RR overexpression on stress related gene expression due to hypoxia.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-02-25 03:22:48"}
  • {"files"=>["https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/1398112/Figure_S1.docx", "https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/1398113/Table_S1.docx", "https://s3-eu-west-1.amazonaws.com/pstorage-plos-3567654/1398114/Table_S2.docx"], "description"=>"<div><p>The (pro)renin-renin receptor, (P)RR has been claimed to be a novel element of the renin-angiotensin system (RAS). The function of (P)RR has been widely studied in renal and vascular pathology but the cardio-specific function of (P)RR has not been studied in detail. We therefore generated a transgenic mouse (Tg) with cardio-restricted (P)RR overexpression driven by the alpha-MHC promotor. The <i>mRNA</i> expression of <i>(P)RR</i> was ∼170-fold higher (P<0.001) and protein expression ∼5-fold higher (P<0.001) in hearts of Tg mice as compared to non-transgenic (wild type, Wt) littermates. This level of overexpression was not associated with spontaneous cardiac morphological or functional abnormalities in Tg mice. To assess whether (P)RR could play a role in cardiac hypertrophy, we infused ISO for 28 days, but this caused an equal degree of cardiac hypertrophy and fibrosis in Wt and Tg mice. In addition, ischemia-reperfusion injury was performed in Langendorff perfused isolated mouse hearts. We did not observe differences in parameters of cardiac function or damage between Wt and Tg mouse hearts under these conditions. Finally, we explored whether the hypoxia sensing response would be modulated by (P)RR using HeLa cells with and without (P)RR overexpression. We did not establish any effect of (P)RR on expression of genes associated with the hypoxic response. These results demonstrate that cardio-specific overexpression of (P)RR does not provoke phenotypical differences in the heart, and does not affect the hearts’ response to stress and injury. It is concluded that increased myocardial (P)RR expression is unlikely to have a major role in pathological cardiac remodeling.</p></div>", "links"=>[], "tags"=>["Anatomy and physiology", "cardiovascular system", "biotechnology", "Genetic engineering", "transgenics", "Model organisms", "Animal models", "mouse", "cardiovascular", "Heart failure", "hypertension", "maintained", "cardiorestricted", "overexpression", "prorenin-renin"], "article_id"=>944246, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Hasan Mahmud", "Wellington Mardoqueu Candido", "Linda van Genne", "Inge Vreeswijk-Baudoin", "Hongjuan Yu", "Bart van de Sluis", "Jan van Deursen", "Wiek H. van Gilst", "Herman H. W. Silljé", "Rudolf A. de Boer"], "doi"=>["http://dx.doi.org/10.1371/journal.pone.0089929"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"http://figshare.com/articles/_Cardiac_Function_and_Architecture_Are_Maintained_in_a_Model_of_Cardiorestricted_Overexpression_of_the_Prorenin_Renin_Receptor_/944246", "title"=>"Cardiac Function and Architecture Are Maintained in a Model of Cardiorestricted Overexpression of the Prorenin-Renin Receptor", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-02-25 03:22:48"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"48", "full-text"=>"41", "pdf"=>"33", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"11", "supp-data"=>"12", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"25", "full-text"=>"22", "pdf"=>"40", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"18", "full-text"=>"17", "pdf"=>"22", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"16", "full-text"=>"12", "pdf"=>"13", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"11", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"10", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"11", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"15", "full-text"=>"9", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"18", "full-text"=>"16", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"15", "full-text"=>"11", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"14", "full-text"=>"20", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"14", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"10", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"16", "full-text"=>"18", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"5", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"14", "full-text"=>"10", "pdf"=>"5", "abstract"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"8", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"18", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"18", "full-text"=>"20", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"12", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"8", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"11", "full-text"=>"15", "pdf"=>"11", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"20", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"10", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"10", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"7", "pdf"=>"6", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"4", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"4", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences/Anatomy", "average_usage"=>[267]}, {"subject_area"=>"/Medicine and health sciences", "average_usage"=>[285]}, {"subject_area"=>"/Medicine and health sciences/Critical care and emergency medicine", "average_usage"=>[245]}, {"subject_area"=>"/Medicine and health sciences/Vascular medicine", "average_usage"=>[248]}]}
Loading … Spinner
There are currently no alerts