Inhibition of Retinoblastoma mRNA Degradation through Poly (A) Involved in the Neuroprotective Effect of Berberine against Cerebral Ischemia
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{"title"=>"Inhibition of retinoblastoma mRNA degradation through poly (A) involved in the neuroprotective effect of berberine against cerebral ischemia", "type"=>"journal", "authors"=>[{"first_name"=>"Yu Shuang", "last_name"=>"Chai", "scopus_author_id"=>"50261193400"}, {"first_name"=>"Zhi Yi", "last_name"=>"Yuan", "scopus_author_id"=>"37038741700"}, {"first_name"=>"Fan", "last_name"=>"Lei", "scopus_author_id"=>"7006258730"}, {"first_name"=>"Yu Gang", "last_name"=>"Wang", "scopus_author_id"=>"56090071800"}, {"first_name"=>"Jun", "last_name"=>"Hu", "scopus_author_id"=>"57190491401"}, {"first_name"=>"Feng", "last_name"=>"Du", "scopus_author_id"=>"55557886000"}, {"first_name"=>"Xi", "last_name"=>"Lu", "scopus_author_id"=>"55557408200"}, {"first_name"=>"Jing Fei", "last_name"=>"Jiang", "scopus_author_id"=>"55557091100"}, {"first_name"=>"Dong Ming", "last_name"=>"Xing", "scopus_author_id"=>"8238952300"}, {"first_name"=>"Li Jun", "last_name"=>"Du", "scopus_author_id"=>"56563434300"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"372725716", "sgr"=>"84897149048", "issn"=>"19326203", "pmid"=>"24603897", "scopus"=>"2-s2.0-84897149048", "doi"=>"10.1371/journal.pone.0090850"}, "id"=>"c7387496-0a03-3204-a545-83a076c9e069", "abstract"=>"Berberine is one kind of isoquinoline alkaloid with anti-apoptotic effects on the neurons suffering ischemia. To address the explanation for these activities, the berberine-induced cell cycle arrest during neurons suffering ischemia/reperfusion had been studied in the present study. According to the in vitro neurons with oxygen-glucose deprivation and in vivo ICR mice with cerebral ischemia/reperfusion, it was found that berberine could protect the mRNA of retinoblastoma (Rb) from degradation through its function on the poly(A) tail. The prolonged half-life of retinoblastoma 1 (gene of Rb, RB1) mRNA level secures the protein level of retinoblastoma, which facilitates cell cycle arrest of neurons in the process of ischemia/reperfusion and subsequently avoids cells entering in the apoptotic process. The poly(A) tail of RB1 mRNA, as a newly identified target of berberine, could help people focus on the interaction between berberine and mRNA to further understand the biological activities and functions of berberine.", "link"=>"http://www.mendeley.com/research/inhibition-retinoblastoma-mrna-degradation-through-poly-involved-neuroprotective-effect-berberine-ag", "reader_count"=>6, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Student > Ph. D. Student"=>4, "Student > Master"=>1}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Student > Ph. D. Student"=>4, "Student > Master"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>1, "Agricultural and Biological Sciences"=>4, "Pharmacology, Toxicology and Pharmaceutical Science"=>1}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Unspecified"=>{"Unspecified"=>1}, "Pharmacology, Toxicology and Pharmaceutical Science"=>{"Pharmacology, Toxicology and Pharmaceutical Science"=>1}}, "reader_count_by_country"=>{"India"=>1}, "group_count"=>0}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1411014"], "description"=>"<p>Berberine (BBR) (0.5 µg/ml) was added in the culture 1 h before OGD treatment and throughout reperfusion. Data are presented as mean ± S.D. of three independent experiments (n = 3). # P<0.05, ## P<0.01 v.s. normal groups; *P<0.05, **P<0.01 v.s. OGD groups.</p>", "links"=>[], "tags"=>["Biochemistry", "Small molecules", "Model organisms", "Animal models", "mouse", "Molecular cell biology", "Cellular types", "neurons", "gene expression", "RNA stability", "Cell growth", "Clinical research design", "Animal models of disease", "Drugs and devices", "Ethnopharmacology", "Neuropharmacology", "neurology", "Cerebrovascular diseases", "Ischemic stroke", "rb", "phosphor-rb"], "article_id"=>954624, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Yu-Shuang Chai", "Zhi-Yi Yuan", "Fan Lei", "Yu-Gang Wang", "Jun Hu", "Feng Du", "Xi Lu", "Jing-Fei Jiang", "Dong-Ming Xing", "Li-Jun Du"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0090850.g003", "stats"=>{"downloads"=>0, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expressions_of_Rb_and_phosphor_Rb_p_Rb_in_primary_neurons_as_measured_by_Western_blot_/954624", "title"=>"Expressions of Rb and phosphor-Rb (p-Rb) in primary neurons as measured by Western blot.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-06 04:30:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/1411017"], "description"=>"<p>Berberine (BBR) was administrated by intraperitoneal injection with three different dosages (1, 2 and 4 mg/kg). Data are presented as mean ± S.D. of six mice (n = 6). ## P<0.01 v.s. control groups; *P<0.05, **P<0.01 v.s. model groups.</p>", "links"=>[], "tags"=>["Biochemistry", "Small molecules", "Model organisms", "Animal models", "mouse", "Molecular cell biology", "Cellular types", "neurons", "gene expression", "RNA stability", "Cell growth", "Clinical research design", "Animal models of disease", "Drugs and devices", "Ethnopharmacology", "Neuropharmacology", "neurology", "Cerebrovascular diseases", "Ischemic stroke", "rb", "phosphor-rb", "hippocampus", "cerebral", "ischemia-treated", "mice"], "article_id"=>954627, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Yu-Shuang Chai", "Zhi-Yi Yuan", "Fan Lei", "Yu-Gang Wang", "Jun Hu", "Feng Du", "Xi Lu", "Jing-Fei Jiang", "Dong-Ming Xing", "Li-Jun Du"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0090850.g004", "stats"=>{"downloads"=>0, "page_views"=>26, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expressions_of_Rb_and_phosphor_Rb_p_Rb_in_the_hippocampus_of_cerebral_ischemia_treated_mice_as_measured_by_Western_blot_/954627", "title"=>"Expressions of Rb and phosphor-Rb (p-Rb) in the hippocampus of cerebral ischemia-treated mice as measured by Western blot.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-06 04:30:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/1411018"], "description"=>"<p>(A) Expression of Rb in normal and RB1 knockdown cells. RB1shRNA was constructed to lentiviral vector PLL3.7. (B) Cell viabilities of normal and RB1 knockdown cells. Columns represent the mean ± S.D. ## P<0.01 v.s. control groups; *P<0.05 v.s. model groups (n = 6). (C) Phases of cell cycle were detected in normal and RB1 knockdown cells. (D) Apoptosis of BBR after OGD treated cells using Annexin V-FITC double staining were detected by using flow cytometry. Data are presented as mean ± S.D. ## P<0.01 v.s. control groups; *P<0.05, **P<0.01 v.s. the OGD (n = 6). (E)–(F) Expression of Rb in cells infected with carrying RB1 shRNAlentivirus under normal and OGD conditions. BBR (0.5 µg/ml) was added in the culture 1 h before OGD treatment and throughout reperfusion. Data are presented as mean ± S.D. of three independent experiments (n = 3). ## P<0.01 v.s. the normal; **P<0.01 v.s. the OGD.</p>", "links"=>[], "tags"=>["Biochemistry", "Small molecules", "Model organisms", "Animal models", "mouse", "Molecular cell biology", "Cellular types", "neurons", "gene expression", "RNA stability", "Cell growth", "Clinical research design", "Animal models of disease", "Drugs and devices", "Ethnopharmacology", "Neuropharmacology", "neurology", "Cerebrovascular diseases", "Ischemic stroke", "berberine", "pc12", "cells", "rb1"], "article_id"=>954628, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Yu-Shuang Chai", "Zhi-Yi Yuan", "Fan Lei", "Yu-Gang Wang", "Jun Hu", "Feng Du", "Xi Lu", "Jing-Fei Jiang", "Dong-Ming Xing", "Li-Jun Du"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0090850.g005", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_berberine_BBR_on_the_PC12_cells_with_RB1_knockdown_/954628", "title"=>"Effects of berberine (BBR) on the PC12 cells with RB1 knockdown.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-06 04:30:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/1411021"], "description"=>"<p>(A) RB1 mRNA level in primary neurons as measured using real-time PCR. BBR (0.5 µg/ml) was added in the culture 1 h before OGD treatment and throughout the reperfusion time. Data represent the mean ± S.D. of three independent experiments (n = 3). # P<0.05, ## P<0.01 v.s. the normal; *P<0.05, **P<0.01 v.s. the OGD. (B) RB1 mRNA level in the hippocampus of cerebral ischemia mice as measured using real-time PCR. BBR was administrated by intraperitoneal injection with three different dosages (1, 2, 4 mg/kg). Data represent the mean ± S.D. of six mice (n = 6). # P<0.05, ## P<0.01 v.s. the control; *P<0.05, **P<0.01 v.s. the model. (C)–(E) RB1 promoter. (C) Overlay of the fluorescence intensity of GFP with RB1 promoter. (D) Quantity of the fluorescence intensity of GFP. (E) GFP mRNA level started by RB1 promoter detected using real-time PCR. Data are presented as mean ± S.D. of three independent experiments (n = 3). ## P<0.01 v.s. the control.</p>", "links"=>[], "tags"=>["Biochemistry", "Small molecules", "Model organisms", "Animal models", "mouse", "Molecular cell biology", "Cellular types", "neurons", "gene expression", "RNA stability", "Cell growth", "Clinical research design", "Animal models of disease", "Drugs and devices", "Ethnopharmacology", "Neuropharmacology", "neurology", "Cerebrovascular diseases", "Ischemic stroke", "up-regulated", "rb1"], "article_id"=>954631, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Yu-Shuang Chai", "Zhi-Yi Yuan", "Fan Lei", "Yu-Gang Wang", "Jun Hu", "Feng Du", "Xi Lu", "Jing-Fei Jiang", "Dong-Ming Xing", "Li-Jun Du"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0090850.g006", "stats"=>{"downloads"=>0, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Berberine_BBR_up_regulated_the_RB1_mRNA_but_couldn_8217_t_influence_the_activity_of_RB1_promoter_/954631", "title"=>"Berberine (BBR) up-regulated the RB1 mRNA, but couldn’t influence the activity of RB1 promoter.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-06 04:30:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/1411022"], "description"=>"<p>(A) Image was taken by using EMSA. (B) Suppressive curve of BBR on the combination between SP1 protein and GC box. (C) PCR detection of the GC box-containing DNA fragment of the RB1 promoter bound by SP1 protein in living cells using ChIP. (D) The quantitative analysis of the GC box-containing DNA fragment of the RB1 promoter combined by SP1 detected using real-time PCR. BBR was 0.5 µg/ml. Data are presented as mean ± S.D. of three independent experiments (n = 3). **P<0.01.</p>", "links"=>[], "tags"=>["Biochemistry", "Small molecules", "Model organisms", "Animal models", "mouse", "Molecular cell biology", "Cellular types", "neurons", "gene expression", "RNA stability", "Cell growth", "Clinical research design", "Animal models of disease", "Drugs and devices", "Ethnopharmacology", "Neuropharmacology", "neurology", "Cerebrovascular diseases", "Ischemic stroke", "berberine", "sp1", "gc", "rb1"], "article_id"=>954632, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Yu-Shuang Chai", "Zhi-Yi Yuan", "Fan Lei", "Yu-Gang Wang", "Jun Hu", "Feng Du", "Xi Lu", "Jing-Fei Jiang", "Dong-Ming Xing", "Li-Jun Du"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0090850.g007", "stats"=>{"downloads"=>3, "page_views"=>22, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_berberine_BBR_on_the_combination_of_SP1_protein_with_the_GC_box_in_RB1_promoter_/954632", "title"=>"Effects of berberine (BBR) on the combination of SP1 protein with the GC box in RB1 promoter.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-06 04:30:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/1411023"], "description"=>"<p>(A) The time-dependent degradation of RB1 mRNA level detected using real-time PCR. (B) The time-dependent degradation of RB1 mRNA level with OGD detected using real-time PCR. (C) The experimental schedule. BBR (0.5 µg/ml) was added in the culture 1 h before OGD treatment and throughout the reperfusion time. Actinomycin D (ACTD) was 2 µg/ml. After ACTD added in culture, the cells were harvested at 0 h, 1 h, 2 h, 3 h, and 4 h for RNA detection. Data were presented as ratio to that of 0 h (ΔCp/Cp0 h). The linear equations are as follows: (A) Control, y = −0.075x+1.104, R2 = 0.9505; Control+BBR, y = −0.039x+1.038, R2 = 0.9839. (B) OGD, y = −0.206x+1.17, R2 = 0.9679; OGD+BBR, y = −0.131x+1.085, R2 = 0.9439. Data are presented as mean ± S.D. from three independent experiments (n = 3). *P<0.05, **P<0.01 v.s. OGD groups.</p>", "links"=>[], "tags"=>["Biochemistry", "Small molecules", "Model organisms", "Animal models", "mouse", "Molecular cell biology", "Cellular types", "neurons", "gene expression", "RNA stability", "Cell growth", "Clinical research design", "Animal models of disease", "Drugs and devices", "Ethnopharmacology", "Neuropharmacology", "neurology", "Cerebrovascular diseases", "Ischemic stroke", "berberine", "degradation", "rb1", "mrna", "ogd"], "article_id"=>954633, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Yu-Shuang Chai", "Zhi-Yi Yuan", "Fan Lei", "Yu-Gang Wang", "Jun Hu", "Feng Du", "Xi Lu", "Jing-Fei Jiang", "Dong-Ming Xing", "Li-Jun Du"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0090850.g008", "stats"=>{"downloads"=>0, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_berberine_BBR_on_the_degradation_of_RB1_mRNA_in_normal_and_OGD_conditions_/954633", "title"=>"Effects of berberine (BBR) on the degradation of RB1 mRNA in normal and OGD conditions.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-06 04:30:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/1411025"], "description"=>"<p>(A) Cartoon of the plasmid models used in this study. Polyadenylation signals were showed in red. (B) Expressions of GFP in cells with transfection of RB1-promoter-RB1-GFP with and without poly(A) plasmids detected using Western blot. (C) Expressions of β-actin and RB1 mRNA using random primer and oligo d(T) in the cells with transfection of RB1-promoter-RB1-GFP with and without poly(A) plasmids detected using RT PCR. (D) Fluorescence intensity in cells with transfection of RB1-promoter-RB1-GFP with and without poly(A) plasmids detected using flow cytometry. (E) RB1 mRNA expressions using oligo d(T) as the primer in cells with transfection of RB1-promoter-RB1-GFP with and without poly(A) plasmids detected using real time PCR. (F) RB1 mRNA level in PC12 cells in normal conditions with transfection of RB1-promoter-RB1-GFP with and without poly(A) plasmids administrated with or without BBR. (J) RB1 mRNA level in PC12 cells in oxygen and glucose deprivation (OGD) conditions with transfection of RB1-promoter-RB1-GFP with and without poly(A) plasmids administrated with or without BBR. (H) The fluorescence spectrum of BBR associating with poly(A) (40 µM). BBR was 0.5 µg/ml. Data are presented as mean ± S.D. from three independent experiments (n = 3). **P<0.01.</p>", "links"=>[], "tags"=>["Biochemistry", "Small molecules", "Model organisms", "Animal models", "mouse", "Molecular cell biology", "Cellular types", "neurons", "gene expression", "RNA stability", "Cell growth", "Clinical research design", "Animal models of disease", "Drugs and devices", "Ethnopharmacology", "Neuropharmacology", "neurology", "Cerebrovascular diseases", "Ischemic stroke", "berberine", "inhibit", "degradation", "rb1", "mrna"], "article_id"=>954635, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Yu-Shuang Chai", "Zhi-Yi Yuan", "Fan Lei", "Yu-Gang Wang", "Jun Hu", "Feng Du", "Xi Lu", "Jing-Fei Jiang", "Dong-Ming Xing", "Li-Jun Du"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0090850.g009", "stats"=>{"downloads"=>1, "page_views"=>31, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effects_of_berberine_BBR_to_inhibit_the_degradation_of_RB1_mRNA_with_poly_A_RB1_pro_RB1_or_without_poly_A_RB1_pro_RB1_916_poly_A_/954635", "title"=>"Effects of berberine (BBR) to inhibit the degradation of RB1 mRNA with poly(A) (RB1-pro-RB1) or without poly(A) (RB1-pro-RB1-Δpoly A-).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-06 04:30:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/1411011"], "description"=>"<p>(A) Protective effect of different concentrations of BBR in PC12 cells after OGD as indicated by MTT assay. (B)–(C) Anti-apoptotic effects of BBR in PC12 cells subjected to OGD as indicated using Annexin V-FITC double staining and flow cytometry. (D)–(E) Effects of BBR on cell cycle arrest in PC12 cells subjected to OGD as indicated by flow cytometry. (F) The cell growth curves with and without BBR in different days. The final concentration of BBR was 0.5 µg/ml. BBR was added to the culture 1 h before OGD treatment and throughout reperfusion. # P<0.05 v.s. the control, ## P<0.01 v.s. the control; *P<0.05, **<0.01 v.s. the OGD. Data are presented as mean ± S.D. of six independent experiments (n = 6).</p>", "links"=>[], "tags"=>["Biochemistry", "Small molecules", "Model organisms", "Animal models", "mouse", "Molecular cell biology", "Cellular types", "neurons", "gene expression", "RNA stability", "Cell growth", "Clinical research design", "Animal models of disease", "Drugs and devices", "Ethnopharmacology", "Neuropharmacology", "neurology", "Cerebrovascular diseases", "Ischemic stroke", "berberine", "pc12", "cells", "subjected", "glucose", "deprivation"], "article_id"=>954621, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Yu-Shuang Chai", "Zhi-Yi Yuan", "Fan Lei", "Yu-Gang Wang", "Jun Hu", "Feng Du", "Xi Lu", "Jing-Fei Jiang", "Dong-Ming Xing", "Li-Jun Du"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0090850.g001", "stats"=>{"downloads"=>0, "page_views"=>15, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Protective_effects_of_berberine_BBR_in_PC12_cells_subjected_to_oxygen_and_glucose_deprivation_OGD_/954621", "title"=>"Protective effects of berberine (BBR) in PC12 cells subjected to oxygen and glucose deprivation (OGD).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-06 04:30:26"}
  • {"files"=>["https://ndownloader.figshare.com/files/1411013"], "description"=>"<p>(A) Expression of Rb proteins and time schedule of the experiment. (B) The percentage of cells in the G1 phase. (C) Total Rb protein. (D) Ratio of phosphor-Rb (S795) to total Rb (phosphor-Rb/Rb). Berberine (0.5 µg/ml) was added to the culture 1 h before OGD treatment and throughout reperfusion. Data are presented as mean ± S.D. of three independent experiments (n = 3).</p>", "links"=>[], "tags"=>["Biochemistry", "Small molecules", "Model organisms", "Animal models", "mouse", "Molecular cell biology", "Cellular types", "neurons", "gene expression", "RNA stability", "Cell growth", "Clinical research design", "Animal models of disease", "Drugs and devices", "Ethnopharmacology", "Neuropharmacology", "neurology", "Cerebrovascular diseases", "Ischemic stroke", "berberine", "cycles", "rb", "expressions", "pc12", "cells", "reperfusion"], "article_id"=>954623, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Yu-Shuang Chai", "Zhi-Yi Yuan", "Fan Lei", "Yu-Gang Wang", "Jun Hu", "Feng Du", "Xi Lu", "Jing-Fei Jiang", "Dong-Ming Xing", "Li-Jun Du"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0090850.g002", "stats"=>{"downloads"=>0, "page_views"=>21, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_berberine_BBR_on_the_cell_cycles_and_Rb_expressions_in_PC12_cells_during_the_6_h_of_reperfusion_after_2_h_of_OGD_/954623", "title"=>"Effect of berberine (BBR) on the cell cycles and Rb expressions in PC12 cells during the 6 h of reperfusion after 2 h of OGD.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-06 04:30:26"}

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Relative Metric

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