Emergence of Carbapenem Resistance Due to the Novel Insertion Sequence ISPa8 in Pseudomonas aeruginosa
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Emergence of carbapenem resistance due to the novel insertion sequence is Pa8 in pseudomonas aeruginosa", "type"=>"journal", "authors"=>[{"first_name"=>"Randal C.", "last_name"=>"Fowler", "scopus_author_id"=>"56096574500"}, {"first_name"=>"Nancy D.", "last_name"=>"Hanson", "scopus_author_id"=>"7005154634"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"372755295", "issn"=>"19326203", "doi"=>"10.1371/journal.pone.0091299", "scopus"=>"2-s2.0-84897497693", "pmid"=>"24614163", "sgr"=>"84897497693"}, "id"=>"3d640142-0431-3ddf-9d7b-6738e404cd46", "abstract"=>"Chronic lung infections due to the persistence of Pseudomonas aeruginosa in cystic fibrosis patients are typically associated with the emergence of antibiotic resistance. The purpose of this study was to investigate the mechanisms responsible for the emergence of carbapenem resistance when a clinical isolate of P. aeruginosa collected from a patient with cystic fibrosis was challenged with meropenem. Nine carbapenem-resistant mutants were selected with subinhibitory concentrations of meropenem from a clinical isolate of P. aeruginosa and characterized for carbapenem resistance. Increased carbapenem MICs were associated with the identification of the novel insertion sequence ISPa8 within oprD or its promoter region in all the mutants. The position of ISPa8 was different for each of the mutants evaluated. In addition, Southern blot analyses identified multiple copies of ISPa8 within the genomes of the mutants and their parent isolate. These data demonstrate that transposition of IS elements within the Pseudomonas genome can influence antibiotic susceptibility. Understanding the selective pressures associated with the emergence of antibiotic resistance is critical for the judicious use of antimicrobial chemotherapy and the successful treatment of bacterial infections.", "link"=>"http://www.mendeley.com/research/emergence-carbapenem-resistance-due-novel-insertion-sequence-pa8-pseudomonas-aeruginosa", "reader_count"=>21, "reader_count_by_academic_status"=>{"Researcher"=>4, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>2, "Student > Master"=>6, "Student > Bachelor"=>2, "Professor"=>1}, "reader_count_by_user_role"=>{"Researcher"=>4, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>2, "Student > Master"=>6, "Student > Bachelor"=>2, "Professor"=>1}, "reader_count_by_subject_area"=>{"Biochemistry, Genetics and Molecular Biology"=>6, "Medicine and Dentistry"=>3, "Agricultural and Biological Sciences"=>9, "Social Sciences"=>1, "Immunology and Microbiology"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>3}, "Social Sciences"=>{"Social Sciences"=>1}, "Immunology and Microbiology"=>{"Immunology and Microbiology"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>9}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>6}}, "reader_count_by_country"=>{"United States"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84897497693"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84897497693?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84897497693&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84897497693&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84897497693", "dc:identifier"=>"SCOPUS_ID:84897497693", "eid"=>"2-s2.0-84897497693", "dc:title"=>"Emergence of carbapenem resistance due to the novel insertion sequence is Pa8 in pseudomonas aeruginosa", "dc:creator"=>"Fowler R.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"9", "prism:issueIdentifier"=>"3", "prism:pageRange"=>nil, "prism:coverDate"=>"2014-04-10", "prism:coverDisplayDate"=>"10 April 2014", "prism:doi"=>"10.1371/journal.pone.0091299", "citedby-count"=>"14", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Creighton University School of Medicine", "affiliation-city"=>"Omaha", "affiliation-country"=>"United States"}], "pubmed-id"=>"24614163", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e91299", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0091299", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"42", "xml_views"=>"6", "html_views"=>"239"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"51", "xml_views"=>"4", "html_views"=>"151"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"30", "xml_views"=>"2", "html_views"=>"118"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"18", "xml_views"=>"1", "html_views"=>"88"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"73"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"3", "html_views"=>"79"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"25", "xml_views"=>"1", "html_views"=>"104"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"20", "xml_views"=>"1", "html_views"=>"99"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"21", "xml_views"=>"2", "html_views"=>"100"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"17", "xml_views"=>"1", "html_views"=>"56"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"16", "xml_views"=>"1", "html_views"=>"62"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"53"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"76"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"1", "html_views"=>"52"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"39"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"40"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"48"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"12", "xml_views"=>"1", "html_views"=>"36"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"20", "xml_views"=>"0", "html_views"=>"52"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"64"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"41"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"46"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"45"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"37"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"14", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"35"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"26"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"8", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"1", "html_views"=>"23"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"21"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"44"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"36"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"13", "xml_views"=>"0", "html_views"=>"54"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"12", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"2", "html_views"=>"20"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"25"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"11"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"40"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"0", "html_views"=>"65"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"2", "html_views"=>"34"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"18"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"5"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"3", "html_views"=>"18"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"9"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"4"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"6"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"5"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"16"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"9", "year"=>"2019", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"10", "year"=>"2019", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"6"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1413609"], "description"=>"a<p>WT, wild-type isolate; CF, clinical isolate from cystic fibrosis patient; P, parent isolate; M, mutant of PA42.</p>b<p>Subinhibitory concentration (µg/mL) of meropenem used for mutant selection.</p>c<p>IPM, imipenem; MEM, meropenem; DOR, doripenem.</p>", "links"=>[], "tags"=>["microbiology", "Bacterial pathogens", "bacteriology", "Microbial mutation", "Microbial pathogens", "Clinical genetics", "Autosomal recessive", "cystic fibrosis", "Infectious diseases", "Bacterial diseases", "Pseudomonas infections", "Infectious disease control", "susceptibility"], "article_id"=>956704, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Randal C. Fowler", "Nancy D. Hanson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091299.t001", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Carbapenem_susceptibility_and_gene_expression_data_for_P_aeruginosa_isolates_/956704", "title"=>"Carbapenem susceptibility and gene expression data for <i>P. aeruginosa</i> isolates.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-03-10 03:10:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/1413607"], "description"=>"<p>(A) PFGE gel of <i>Spe</i>I chromosomal digests of wild-type strain PAO1, parent isolate PA42, and mutant 812M visualized using SYBR gold. (B) Southern blot of the gel depicted in <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0091299#pone-0091299-g005\" target=\"_blank\">Figure 5(A)</a> using an IS<i>Pa</i>8-specific probe. Lane 1, PAO1 (negative control); lane 2, parent isolate PA42; lane 3, mutant 812M.</p>", "links"=>[], "tags"=>["microbiology", "Bacterial pathogens", "bacteriology", "Microbial mutation", "Microbial pathogens", "Clinical genetics", "Autosomal recessive", "cystic fibrosis", "Infectious diseases", "Bacterial diseases", "Pseudomonas infections", "Infectious disease control", "gel", "electrophoresis", "blot"], "article_id"=>956702, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Randal C. Fowler", "Nancy D. Hanson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091299.g005", "stats"=>{"downloads"=>2, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Pulsed_Field_Gel_Electrophoresis_and_Southern_blot_analyses_using_IS_Pa_8_specific_probe_/956702", "title"=>"Pulsed Field Gel Electrophoresis and Southern blot analyses using IS<i>Pa</i>8-specific probe.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-10 03:10:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/1413590"], "description"=>"<p>The solid black arrows represent the <i>oprD</i> gene while the green arrows represent the location and orientation of IS<i>Pa</i>8 within <i>oprD</i>. The downward arrow indicates the predicted insertion site of IS<i>Pa</i>8 within <i>oprD</i> as determined by sequence analysis.</p>", "links"=>[], "tags"=>["microbiology", "Bacterial pathogens", "bacteriology", "Microbial mutation", "Microbial pathogens", "Clinical genetics", "Autosomal recessive", "cystic fibrosis", "Infectious diseases", "Bacterial diseases", "Pseudomonas infections", "Infectious disease control", "sites", "pa42", "isogenic"], "article_id"=>956699, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Randal C. Fowler", "Nancy D. Hanson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091299.g002", "stats"=>{"downloads"=>1, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Insertion_sites_of_IS_Pa_8_with_respect_to_the_oprD_gene_in_PA42_and_nine_isogenic_mutants_/956699", "title"=>"Insertion sites of IS<i>Pa</i>8 with respect to the <i>oprD</i> gene in PA42 and nine isogenic mutants.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-10 03:10:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/1413610", "https://ndownloader.figshare.com/files/1413612", "https://ndownloader.figshare.com/files/1413613"], "description"=>"<div><p>Chronic lung infections due to the persistence of <i>Pseudomonas aeruginosa</i> in cystic fibrosis patients are typically associated with the emergence of antibiotic resistance. The purpose of this study was to investigate the mechanisms responsible for the emergence of carbapenem resistance when a clinical isolate of <i>P. aeruginosa</i> collected from a patient with cystic fibrosis was challenged with meropenem. Nine carbapenem-resistant mutants were selected with subinhibitory concentrations of meropenem from a clinical isolate of <i>P. aeruginosa</i> and characterized for carbapenem resistance. Increased carbapenem MICs were associated with the identification of the novel insertion sequence IS<i>Pa</i>8 within <i>oprD</i> or its promoter region in all the mutants. The position of IS<i>Pa</i>8 was different for each of the mutants evaluated. In addition, Southern blot analyses identified multiple copies of IS<i>Pa</i>8 within the genomes of the mutants and their parent isolate. These data demonstrate that transposition of IS elements within the <i>Pseudomonas</i> genome can influence antibiotic susceptibility. Understanding the selective pressures associated with the emergence of antibiotic resistance is critical for the judicious use of antimicrobial chemotherapy and the successful treatment of bacterial infections.</p></div>", "links"=>[], "tags"=>["microbiology", "Bacterial pathogens", "bacteriology", "Microbial mutation", "Microbial pathogens", "Clinical genetics", "Autosomal recessive", "cystic fibrosis", "Infectious diseases", "Bacterial diseases", "Pseudomonas infections", "Infectious disease control", "emergence", "carbapenem", "insertion"], "article_id"=>956706, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Randal C. Fowler", "Nancy D. Hanson"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0091299.s001", "https://dx.doi.org/10.1371/journal.pone.0091299.s002", "https://dx.doi.org/10.1371/journal.pone.0091299.s003"], "stats"=>{"downloads"=>14, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/Emergence_of_Carbapenem_Resistance_Due_to_the_Novel_Insertion_Sequence_IS_Pa_8_in_Pseudomonas_aeruginosa_/956706", "title"=>"Emergence of Carbapenem Resistance Due to the Novel Insertion Sequence IS<i>Pa</i>8 in <i>Pseudomonas aeruginosa</i>", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-03-10 03:10:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/1413606"], "description"=>"<p>The expected PCR product size is 1,672(1 kb DNA ladder, Invitrogen), no template control (NTC), or <i>P. aeruginosa</i> isolates.</p>", "links"=>[], "tags"=>["microbiology", "Bacterial pathogens", "bacteriology", "Microbial mutation", "Microbial pathogens", "Clinical genetics", "Autosomal recessive", "cystic fibrosis", "Infectious diseases", "Bacterial diseases", "Pseudomonas infections", "Infectious disease control", "amplification", "isogenic"], "article_id"=>956701, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Randal C. Fowler", "Nancy D. Hanson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091299.g004", "stats"=>{"downloads"=>1, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_PCR_amplification_of_the_oprF_gene_in_PAO1_PA42_and_nine_isogenic_mutants_/956701", "title"=>"PCR amplification of the <i>oprF</i> gene in PAO1, PA42, and nine isogenic mutants.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-10 03:10:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/1413598"], "description"=>"<p>Outer membrane profiles were analyzed for the presence of the porin, OprD. PAO1 and a fully susceptible cystic fibrosis isolate, PA443, were included as positive controls. The locations of OprD and OprF proteins are indicated.</p>", "links"=>[], "tags"=>["microbiology", "Bacterial pathogens", "bacteriology", "Microbial mutation", "Microbial pathogens", "Clinical genetics", "Autosomal recessive", "cystic fibrosis", "Infectious diseases", "Bacterial diseases", "Pseudomonas infections", "Infectious disease control", "membrane", "pa42", "isogenic"], "article_id"=>956700, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Randal C. Fowler", "Nancy D. Hanson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091299.g003", "stats"=>{"downloads"=>2, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Outer_membrane_analysis_of_P_aeruginosa_PA42_and_nine_isogenic_mutants_/956700", "title"=>"Outer membrane analysis of <i>P. aeruginosa</i> PA42 and nine isogenic mutants.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-10 03:10:52"}
  • {"files"=>["https://ndownloader.figshare.com/files/1413589"], "description"=>"<p>(A) Primers that flanked the <i>oprD</i> gene were used to PCR-amplify the <i>oprD</i> gene giving an expected PCR product size is 1586 bp. Each lane is labeled with its respective DNA ladder (1 kb DNA ladder, Invitrogen), no template control (NTC), or <i>P. aeruginosa</i> isolate. (B) PCR amplification of IS<i>Pa</i>8 within the <i>oprD</i> gene in the nine carbapenem-resistant mutants. Primers ISPa8F1 and OprDRTR3 were used to map the approximate location of the IS<i>Pa</i>8 within the <i>oprD</i> gene. The smaller PCR products indicate IS<i>Pa</i>8 has inserted near the 3′ end of <i>oprD</i>, while larger PCR products indicate IS<i>Pa</i>8 is inserted near the 5′ end. No PCR product was observed for mutant 711M. (C) Primers OprDRTF2 and ISPa8R2 were used to confirm the location of IS<i>Pa</i>8 within the <i>oprD</i> gene in the nine isogenic mutants. Non-specific bands were amplified in PA42, mutant 711M, and mutant 922M suggesting that multiple IS<i>Pa</i>8 elements may be present within the genome. To confirm that IS<i>Pa</i>8 had inserted within the <i>oprD</i> gene or its flanking regions in mutants 711M and 922M, PCR products for these mutants shown in (A) were sequenced using primers OprDRTF2 or ISPa8F1.</p>", "links"=>[], "tags"=>["microbiology", "Bacterial pathogens", "bacteriology", "Microbial mutation", "Microbial pathogens", "Clinical genetics", "Autosomal recessive", "cystic fibrosis", "Infectious diseases", "Bacterial diseases", "Pseudomonas infections", "Infectious disease control", "amplification", "carbapenem-resistant", "mutants"], "article_id"=>956697, "categories"=>["Biological Sciences", "Medicine"], "users"=>["Randal C. Fowler", "Nancy D. Hanson"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091299.g001", "stats"=>{"downloads"=>2, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_PCR_amplification_of_oprD_and_IS_Pa_8_from_carbapenem_resistant_mutants_of_P_aeruginosa_/956697", "title"=>"PCR amplification of <i>oprD</i> and IS<i>Pa</i>8 from carbapenem-resistant mutants of <i>P. aeruginosa</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-10 03:10:52"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"47", "full-text"=>"54", "pdf"=>"27", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2014", "month"=>"3"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"20", "full-text"=>"20", "pdf"=>"16", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"6", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"41", "full-text"=>"43", "pdf"=>"29", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"9", "full-text"=>"12", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"11", "full-text"=>"12", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"8", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"14", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"17", "full-text"=>"20", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"12", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"2", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"20", "full-text"=>"16", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"15", "full-text"=>"9", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"4", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"11", "full-text"=>"8", "pdf"=>"8", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"8", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"14", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"13", "full-text"=>"12", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"10", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"6", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"8"}
  • {"unique-ip"=>"9", "full-text"=>"9", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"2", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"6", "full-text"=>"4", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"9", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"14", "full-text"=>"7", "pdf"=>"15", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"3", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"4", "pdf"=>"8", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[291]}, {"subject_area"=>"/Biology and life sciences/Developmental biology", "average_usage"=>[285]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[292, 461]}, {"subject_area"=>"/Medicine and health sciences/Clinical genetics", "average_usage"=>[306, 468]}]}
Loading … Spinner
There are currently no alerts