PmVRP15, a Novel Viral Responsive Protein from the Black Tiger Shrimp, Penaeus monodon, Promoted White Spot Syndrome Virus Replication
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{"title"=>"PmVRP15, a novel viral responsive protein from the black tiger shrimp, Penaeus monodon, promoted white spot syndrome virus replication", "type"=>"journal", "authors"=>[{"first_name"=>"Tipachai", "last_name"=>"Vatanavicharn", "scopus_author_id"=>"26430258000"}, {"first_name"=>"Adisak", "last_name"=>"Prapavorarat", "scopus_author_id"=>"35330774700"}, {"first_name"=>"Phattarunda", "last_name"=>"Jaree", "scopus_author_id"=>"55376957900"}, {"first_name"=>"Kunlaya", "last_name"=>"Somboonwiwat", "scopus_author_id"=>"56115070200"}, {"first_name"=>"Anchalee", "last_name"=>"Tassanakajon", "scopus_author_id"=>"6603935274"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"isbn"=>"1932-6203", "pmid"=>"24637711", "doi"=>"10.1371/journal.pone.0091930", "pui"=>"372838397", "issn"=>"19326203", "sgr"=>"84898636045", "scopus"=>"2-s2.0-84898636045"}, "id"=>"fcb4125c-e54c-3adb-88ae-bf17a115b451", "abstract"=>"Suppression subtractive hybridization of Penaeus monodon hemocytes challenged with white spot syndrome virus (WSSV) has identified the viral responsive gene, PmVRP15, as the highest up-regulated gene ever reported in shrimps. Expression analysis by quantitative real time RT-PCR revealed 9410-fold up-regulated level at 48 h post WSSV injection. Tissue distribution analysis showed that PmVRP15 transcript was mainly expressed in the hemocytes of shrimp. The full-length cDNA of PmVRP15 transcript was obtained and showed no significant similarity to any known gene in the GenBank database. The predicted open reading frame of PmVRP15 encodes for a deduced 137 amino acid protein containing a putative transmembrane helix. Immunofluorescent localization of the PmVRP15 protein revealed it accumulated around the nuclear membrane in all three types of shrimp hemocytes and that the protein was highly up-regulated in WSSV-infected shrimps. Double-stranded RNA interference-mediated gene silencing of PmVRP15 in P. monodon significantly decreased WSSV propagation compared to the control shrimps (injected with GFP dsRNA). The significant decrease in cumulative mortality rate of WSSV-infected shrimp following PmVRP15 knockdown was observed. These results suggest that PmVRP15 is likely to be a nuclear membrane protein and that it acts as a part of WSSV propagation pathway.", "link"=>"http://www.mendeley.com/research/pmvrp15-novel-viral-responsive-protein-black-tiger-shrimp-penaeus-monodon-promoted-white-spot-syndro", "reader_count"=>20, "reader_count_by_academic_status"=>{"Librarian"=>1, "Student > Doctoral Student"=>2, "Researcher"=>2, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>1, "Other"=>2, "Student > Master"=>3, "Student > Bachelor"=>1, "Lecturer"=>2, "Professor"=>1}, "reader_count_by_user_role"=>{"Librarian"=>1, "Student > Doctoral Student"=>2, "Researcher"=>2, "Student > Ph. D. Student"=>5, "Student > Postgraduate"=>1, "Other"=>2, "Student > Master"=>3, "Student > Bachelor"=>1, "Lecturer"=>2, "Professor"=>1}, "reader_count_by_subject_area"=>{"Environmental Science"=>1, "Medicine and Dentistry"=>1, "Agricultural and Biological Sciences"=>13, "Social Sciences"=>2, "Computer Science"=>1, "Biochemistry, Genetics and Molecular Biology"=>2}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Social Sciences"=>{"Social Sciences"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>13}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Environmental Science"=>{"Environmental Science"=>1}}, "reader_count_by_country"=>{"Madagascar"=>1}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1423329"], "description"=>"<p>(A) Cumulative mortality of WSSV-infected <i>Pm</i>VRP15 gene knockdown shrimp (black line) was compared with that of the control, WSSV-infected GFP gene knockdown shrimp (Grey line). Data are shown as the mean ±1 S.D. and are derived from three independent repeats. (B) After knockdown <i>Pm</i>VRP15 gene in WSSV-infected shrimp, <i>Pm</i>VRP15 gene recovery was observed after WSSV infection at 24, 36, 48 and 60 hpi.</p>", "links"=>[], "tags"=>["agriculture", "aquaculture", "Shrimp farming", "Biochemistry", "proteins", "Immune system proteins", "cell biology", "Cellular structures and organelles", "Cell membranes", "Membrane proteins", "Transmembrane proteins", "genetics", "gene expression", "immunology", "Immune response", "microbiology", "Virology", "Viral replication", "Viral transmission and infection", "Pathology and laboratory medicine", "pathogenesis", "Host-pathogen interactions", "knockdown", "wssv"], "article_id"=>964373, "categories"=>["Biological Sciences"], "users"=>["Tipachai Vatanavicharn", "Adisak Prapavorarat", "Phattarunda Jaree", "Kunlaya Somboonwiwat", "Anchalee Tassanakajon"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091930.g008", "stats"=>{"downloads"=>1, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_involvement_of_knockdown_Pm_VRP15_gene_in_WSSV_infection_in_shrimp_/964373", "title"=>"The involvement of knockdown <i>Pm</i>VRP15 gene in WSSV infection in shrimp.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-17 03:32:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/1423328"], "description"=>"<p>Transcript expression level of the WSSV genes: <i>ie-1</i>, <i>wsv477</i> and <i>vp28</i>, in <i>Pm</i>VRP15 gene-silenced <i>P. monodon</i> hemocytes were determined by qRT-PCR. Data are shown as the mean ±1 SD of three replicates and as the fold change of <i>ie-1</i>, <i>wsv477</i> and <i>vp28</i> after normalization to the EF-1α transcript levels (grey bar). The control group (GFP-dsRNA injected) are shown in the black bars.</p>", "links"=>[], "tags"=>["agriculture", "aquaculture", "Shrimp farming", "Biochemistry", "proteins", "Immune system proteins", "cell biology", "Cellular structures and organelles", "Cell membranes", "Membrane proteins", "Transmembrane proteins", "genetics", "gene expression", "immunology", "Immune response", "microbiology", "Virology", "Viral replication", "Viral transmission and infection", "Pathology and laboratory medicine", "pathogenesis", "Host-pathogen interactions", "wssv", "propagation"], "article_id"=>964372, "categories"=>["Biological Sciences"], "users"=>["Tipachai Vatanavicharn", "Adisak Prapavorarat", "Phattarunda Jaree", "Kunlaya Somboonwiwat", "Anchalee Tassanakajon"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091930.g007", "stats"=>{"downloads"=>1, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_effect_of_Pm_VRP15_gene_silencing_on_WSSV_propagation_in_P_monodon_hemocytes_/964372", "title"=>"The effect of <i>Pm</i>VRP15 gene silencing on WSSV propagation in <i>P. monodon</i> hemocytes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-17 03:32:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/1423325"], "description"=>"<p>Hemocytes were collected at 48(HLS) was prepared and 70 μg of total HLS protein per track was subjected to duplicate SDS-PAGE resolution. Gels were then either stained with coomassie blue for total protein detection or subject to Western-blot analysis to detect <i>Pm</i>VRP15 and β-actin using specific antibodies. M is the protein size markers.</p>", "links"=>[], "tags"=>["agriculture", "aquaculture", "Shrimp farming", "Biochemistry", "proteins", "Immune system proteins", "cell biology", "Cellular structures and organelles", "Cell membranes", "Membrane proteins", "Transmembrane proteins", "genetics", "gene expression", "immunology", "Immune response", "microbiology", "Virology", "Viral replication", "Viral transmission and infection", "Pathology and laboratory medicine", "pathogenesis", "Host-pathogen interactions", "blot", "wssv-", "injected"], "article_id"=>964369, "categories"=>["Biological Sciences"], "users"=>["Tipachai Vatanavicharn", "Adisak Prapavorarat", "Phattarunda Jaree", "Kunlaya Somboonwiwat", "Anchalee Tassanakajon"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091930.g004", "stats"=>{"downloads"=>1, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Western_blot_analysis_of_Pm_VRP15_native_protein_in_control_NaCl_and_WSSV_injected_WSSV_P_monodon_hemocytes_/964369", "title"=>"Western blot analysis of <i>Pm</i>VRP15 native protein in control (NaCl) and WSSV- injected (WSSV) <i>P. monodon</i> hemocytes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-17 03:32:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/1423323"], "description"=>"<p>The tissues examined were antennal gland (AN), epipodite (EP), eye stalk (ES), gill (G), heart (H), hemocyte (HC), hepatopancreas (HP), intestine (I) and lymphoid (L). EF1-α was used as the internal reference and PCR control.</p>", "links"=>[], "tags"=>["agriculture", "aquaculture", "Shrimp farming", "Biochemistry", "proteins", "Immune system proteins", "cell biology", "Cellular structures and organelles", "Cell membranes", "Membrane proteins", "Transmembrane proteins", "genetics", "gene expression", "immunology", "Immune response", "microbiology", "Virology", "Viral replication", "Viral transmission and infection", "Pathology and laboratory medicine", "pathogenesis", "Host-pathogen interactions", "transcript", "tissues"], "article_id"=>964367, "categories"=>["Biological Sciences"], "users"=>["Tipachai Vatanavicharn", "Adisak Prapavorarat", "Phattarunda Jaree", "Kunlaya Somboonwiwat", "Anchalee Tassanakajon"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091930.g002", "stats"=>{"downloads"=>0, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Pm_VRP15_transcript_expression_analysis_in_various_P_monodon_tissues_by_RT_PCR_/964367", "title"=>"<i>Pm</i>VRP15 transcript expression analysis in various <i>P. monodon</i> tissues by RT-PCR.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-17 03:32:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/1423324"], "description"=>"<p>Relative expression ratios, as determined by qRT-PCR, of <i>Pm</i>VRP15 transcript levels in the hemocytes of WSSV-infected <i>P. monodon</i> were compared to those of the control (non-infected) shrimps and standardized against β-actin as the internal reference, at 24, 48 and 72 hpi with WSSV. The data represent the mean ±1 SD relative expression of <i>Pm</i>VRP15 post-infection (solid bar, right) and the control (open bar, left), derived from three independent experiments. Means with an asterisk are significantly different (<i>P</i><0.05, paired samples <i>t</i>-test). A relative expression ratio of <1, 1 and >1 mean that the gene expression level is down-regulated, the same or up-regulated, respectively, in the hemocytes of WSSV-infected shrimps compared to the uninfected control.</p>", "links"=>[], "tags"=>["agriculture", "aquaculture", "Shrimp farming", "Biochemistry", "proteins", "Immune system proteins", "cell biology", "Cellular structures and organelles", "Cell membranes", "Membrane proteins", "Transmembrane proteins", "genetics", "gene expression", "immunology", "Immune response", "microbiology", "Virology", "Viral replication", "Viral transmission and infection", "Pathology and laboratory medicine", "pathogenesis", "Host-pathogen interactions", "transcripts", "wssv"], "article_id"=>964368, "categories"=>["Biological Sciences"], "users"=>["Tipachai Vatanavicharn", "Adisak Prapavorarat", "Phattarunda Jaree", "Kunlaya Somboonwiwat", "Anchalee Tassanakajon"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091930.g003", "stats"=>{"downloads"=>2, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Up_regulation_of_Pm_VRP15_transcripts_in_response_to_WSSV_infection_/964368", "title"=>"Up-regulation of <i>Pm</i>VRP15 transcripts in response to WSSV infection.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-17 03:32:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/1423322"], "description"=>"<p>The putative start codon (ATG) is in bold, the asterisk indicates the stop codon (TAA, in bold italics), the potential transmembrane domain is boxed and the proposed polyadenylation site is underlined.</p>", "links"=>[], "tags"=>["agriculture", "aquaculture", "Shrimp farming", "Biochemistry", "proteins", "Immune system proteins", "cell biology", "Cellular structures and organelles", "Cell membranes", "Membrane proteins", "Transmembrane proteins", "genetics", "gene expression", "immunology", "Immune response", "microbiology", "Virology", "Viral replication", "Viral transmission and infection", "Pathology and laboratory medicine", "pathogenesis", "Host-pathogen interactions", "cdna", "nucleotide", "deduced", "amino", "sequences", "accession"], "article_id"=>964366, "categories"=>["Biological Sciences"], "users"=>["Tipachai Vatanavicharn", "Adisak Prapavorarat", "Phattarunda Jaree", "Kunlaya Somboonwiwat", "Anchalee Tassanakajon"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091930.g001", "stats"=>{"downloads"=>0, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_cDNA_nucleotide_and_deduced_protein_amino_acid_sequences_of_Pm_VRP15_GenBank_accession_code_KF683338_/964366", "title"=>"The cDNA nucleotide and deduced protein amino acid sequences of <i>Pm</i>VRP15 (GenBank accession code KF683338).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-17 03:32:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/1423330"], "description"=>"<p>Nucleotide sequences of the PCR primers used in this study.</p>", "links"=>[], "tags"=>["agriculture", "aquaculture", "Shrimp farming", "Biochemistry", "proteins", "Immune system proteins", "cell biology", "Cellular structures and organelles", "Cell membranes", "Membrane proteins", "Transmembrane proteins", "genetics", "gene expression", "immunology", "Immune response", "microbiology", "Virology", "Viral replication", "Viral transmission and infection", "Pathology and laboratory medicine", "pathogenesis", "Host-pathogen interactions", "sequences", "pcr", "primers"], "article_id"=>964374, "categories"=>["Biological Sciences"], "users"=>["Tipachai Vatanavicharn", "Adisak Prapavorarat", "Phattarunda Jaree", "Kunlaya Somboonwiwat", "Anchalee Tassanakajon"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091930.t001", "stats"=>{"downloads"=>2, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Nucleotide_sequences_of_the_PCR_primers_used_in_this_study_/964374", "title"=>"Nucleotide sequences of the PCR primers used in this study.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-03-17 03:32:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/1423327"], "description"=>"<p>(A) Transcriptional level of <i>Pm</i>VRP15 transcripts after 24 h post-WSSV infection and -<i>Pm</i>VRP15 gene knockdown in the <i>P. monodon</i> hemocytes was determined by RT-PCR using gene specific primers. The control was shrimp that was injected with GFP dsRNA. Three individuals were used for each group and each experiment was performed in triplicate. (B) Protein expression level of <i>Pm</i>VRP15 was detected in both groups to confirm the success of <i>Pm</i>VRP15 knockdown. Hemocytes were collected at 24 h after <i>Pm</i>VRP15 gene knockdown in WSSV-infected shrimp, 70 μg of total HLS protein was analyzed by SDS-PAGE and western blot analysis using antibody specific to <i>Pm</i>VRP15 and β-actin protein, an internal control.</p>", "links"=>[], "tags"=>["agriculture", "aquaculture", "Shrimp farming", "Biochemistry", "proteins", "Immune system proteins", "cell biology", "Cellular structures and organelles", "Cell membranes", "Membrane proteins", "Transmembrane proteins", "genetics", "gene expression", "immunology", "Immune response", "microbiology", "Virology", "Viral replication", "Viral transmission and infection", "Pathology and laboratory medicine", "pathogenesis", "Host-pathogen interactions"], "article_id"=>964371, "categories"=>["Biological Sciences"], "users"=>["Tipachai Vatanavicharn", "Adisak Prapavorarat", "Phattarunda Jaree", "Kunlaya Somboonwiwat", "Anchalee Tassanakajon"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091930.g006", "stats"=>{"downloads"=>2, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_The_Pm_VRP15_gene_silencing_in_P_monodon_hemocytes_/964371", "title"=>"The <i>Pm</i>VRP15 gene silencing in <i>P. monodon</i> hemocytes.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-17 03:32:09"}
  • {"files"=>["https://ndownloader.figshare.com/files/1423326"], "description"=>"<p>Rabbit anti-r<i>Pm</i>VRP15 and mouse anti-VP28 primary antibodies were detected with corresponding Alexa488 and Alexa568 secondary antibodies revealing <i>Pm</i>VRP15 (green color) and VP28 (red color), respectively. Scale bars represent (A) 5 μm and (B) 2 μm. Nucleus was stained with TO-PRO-3 iodide and color was adjusted to blue. The bright field image showed hyaline cell (HC), semigranular cell (SGC) and granular cell (GC).</p>", "links"=>[], "tags"=>["agriculture", "aquaculture", "Shrimp farming", "Biochemistry", "proteins", "Immune system proteins", "cell biology", "Cellular structures and organelles", "Cell membranes", "Membrane proteins", "Transmembrane proteins", "genetics", "gene expression", "immunology", "Immune response", "microbiology", "Virology", "Viral replication", "Viral transmission and infection", "Pathology and laboratory medicine", "pathogenesis", "Host-pathogen interactions", "images", "uninfected", "wssv-infected", "hemocytes", "48", "hpi"], "article_id"=>964370, "categories"=>["Biological Sciences"], "users"=>["Tipachai Vatanavicharn", "Adisak Prapavorarat", "Phattarunda Jaree", "Kunlaya Somboonwiwat", "Anchalee Tassanakajon"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0091930.g005", "stats"=>{"downloads"=>0, "page_views"=>26, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_CFLM_derived_images_of_the_uninfected_control_and_WSSV_infected_hemocytes_at_48_hpi_with_WSSV_/964370", "title"=>"CFLM-derived images of the uninfected (control) and WSSV-infected hemocytes at 48 hpi with WSSV.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-17 03:32:09"}

PMC Usage Stats | Further Information

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Relative Metric

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