Evaluation of Nanolipoprotein Particles (NLPs) as an In Vivo Delivery Platform
Publication Date
March 27, 2014
Journal
PLOS ONE
Authors
Nicholas O. Fischer, Dina R. Weilhammer, Alexis Dunkle, Cynthia Thomas, et al
Volume
9
Issue
3
Pages
e93342
DOI
https://dx.plos.org/10.1371/journal.pone.0093342
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0093342
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/24675794
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3968139
Europe PMC
http://europepmc.org/abstract/MED/24675794
Web of Science
000333677500110
Scopus
84899806300
Mendeley
http://www.mendeley.com/research/evaluation-nanolipoprotein-particles-nlps-vivo-delivery-platform
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Mendeley | Further Information

{"title"=>"Evaluation of Nanolipoprotein Particles (NLPs) as an in vivo delivery platform", "type"=>"journal", "authors"=>[{"first_name"=>"Nicholas O.", "last_name"=>"Fischer", "scopus_author_id"=>"14044979500"}, {"first_name"=>"Dina R.", "last_name"=>"Weilhammer", "scopus_author_id"=>"55878941200"}, {"first_name"=>"Alexis", "last_name"=>"Dunkle", "scopus_author_id"=>"56674443100"}, {"first_name"=>"Cynthia", "last_name"=>"Thomas", "scopus_author_id"=>"57199114590"}, {"first_name"=>"Mona", "last_name"=>"Hwang", "scopus_author_id"=>"8789713200"}, {"first_name"=>"Michele", "last_name"=>"Corzett", "scopus_author_id"=>"6603689707"}, {"first_name"=>"Cheri", "last_name"=>"Lychak", "scopus_author_id"=>"55862826700"}, {"first_name"=>"Wasima", "last_name"=>"Mayer", "scopus_author_id"=>"57196642986"}, {"first_name"=>"Salustra", "last_name"=>"Urbin", "scopus_author_id"=>"12784633500"}, {"first_name"=>"Nicole", "last_name"=>"Collette", "scopus_author_id"=>"6602733125"}, {"first_name"=>"Jiun Chiun", "last_name"=>"Chang", "scopus_author_id"=>"56149853600"}, {"first_name"=>"Gabriela G.", "last_name"=>"Loots", "scopus_author_id"=>"6604082598"}, {"first_name"=>"Amy", "last_name"=>"Rasley", "scopus_author_id"=>"6506565550"}, {"first_name"=>"Craig D.", "last_name"=>"Blanchette", "scopus_author_id"=>"13104438400"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84899806300", "doi"=>"10.1371/journal.pone.0093342", "pui"=>"373030932", "pmid"=>"24675794", "scopus"=>"2-s2.0-84899806300", "issn"=>"19326203", "isbn"=>"1932-6203"}, "id"=>"acdcede7-c7e0-315a-bce2-29d08645c408", "abstract"=>"Nanoparticles hold great promise for the delivery of therapeutics, yet limitations remain with regards to the use of these nanosystems for efficient long-lasting targeted delivery of therapeutics, including imparting functionality to the platform, in vivo stability, drug entrapment efficiency and toxicity. To begin to address these limitations, we evaluated the functionality, stability, cytotoxicity, toxicity, immunogenicity and in vivo biodistribution of nanolipoprotein particles (NLPs), which are mimetics of naturally occurring high-density lipoproteins (HDLs). We found that a wide range of molecules could be reliably conjugated to the NLP, including proteins, single-stranded DNA, and small molecules. The NLP was also found to be relatively stable in complex biological fluids and displayed no cytotoxicity in vitro at doses as high as 320 µg/ml. In addition, we observed that in vivo administration of the NLP daily for 14 consecutive days did not induce significant weight loss or result in lesions on excised organs. Furthermore, the NLPs did not display overt immunogenicity with respect to antibody generation. Finally, the biodistribution of the NLP in vivo was found to be highly dependent on the route of administration, where intranasal administration resulted in prolonged retention in the lung tissue. Although only a select number of NLP compositions were evaluated, the findings of this study suggest that the NLP platform holds promise for use as both a targeted and non-targeted in vivo delivery vehicle for a range of therapeutics.", "link"=>"http://www.mendeley.com/research/evaluation-nanolipoprotein-particles-nlps-vivo-delivery-platform", "reader_count"=>20, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>7, "Researcher"=>5, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>3, "Student > Master"=>1, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>7, "Researcher"=>5, "Student > Doctoral Student"=>2, "Student > Ph. D. Student"=>3, "Student > Master"=>1, "Other"=>1, "Student > Bachelor"=>1}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Engineering"=>1, "Biochemistry, Genetics and Molecular Biology"=>3, "Materials Science"=>1, "Agricultural and Biological Sciences"=>10, "Medicine and Dentistry"=>1, "Chemistry"=>2}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>1}, "Materials Science"=>{"Materials Science"=>1}, "Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>10}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>3}, "Unspecified"=>{"Unspecified"=>2}}, "group_count"=>1}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1438346"], "description"=>"<p>Weights of A) liver, B) kidney, C) lung, and D) spleen obtained from mice that received 25 µg of NiNLP i.n. (30 µl) or i.p. (100 µl) daily for 14 consecutive days. Control animals received an equal volume of PBS i.p.(100 µl) daily for 14 days. Normalized organ weights are represented as (organ weight, g)/(body weight, g). Data represent averaged organ weights from groups of three animals, with standard deviation error bars.</p>", "links"=>[], "tags"=>["biophysics", "biotechnology", "biomaterials", "Bionanotechnology", "immunology", "nanotechnology", "Clinical medicine", "pharmacology", "Drug research and development", "drug discovery", "pharmacokinetics", "Drug distribution", "Materials Science", "repeated", "ninlp"], "article_id"=>976908, "categories"=>["Biological Sciences"], "users"=>["Nicholas O. Fischer", "Dina R. Weilhammer", "Alexis Dunkle", "Cynthia Thomas", "Mona Hwang", "Michele Corzett", "Cheri Lychak", "Wasima Mayer", "Salustra Urbin", "Nicole Collette", "Jiun Chiun Chang", "Gabriela G. Loots", "Amy Rasley", "Craig D. Blanchette"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093342.g009", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_repeated_NiNLP_administration_on_mouse_organ_weights_/976908", "title"=>"Effect of repeated NiNLP administration on mouse organ weights.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-27 03:27:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1438341"], "description"=>"<p>A) and B) SEC chromatograms of 0841 (black line) and 0841-DBCO (blue line) incubated with AF488-azide at a fluorescent dye to protein ratio of 5 monitored at two absorbance wavelengths: A) 280 nm and B) 495 nm. Chromatogram peaks correspond to 0841 protein (t<sub>R</sub> 12.5 min) and unreacted AF488-azide (t<sub>R</sub> 16.3 min). C) SEC chromatograms of N<sub>3</sub>NLPs incubated with 0841-DBCO at indicated molar ratios. Chromatogram peaks correspond to NLP (t<sub>R</sub> 8.5 min) and unconjugated 0841 protein (t<sub>R</sub> 12.5 min). D) The 0841-DBCO:N<sub>3</sub>NLPs complexes were purified by SEC, and SDS-PAGE and densitometry were used to quantify the amount of 0841 and apoE422k protein in each NiNLP sample using known standards for each protein. These measured concentrations were used to calculate the number of proteins bound per NLP and were plotted as a function of the initial ratio used in the conjugation reaction. E) SEC chromatograms of NLPs (lacking the C18-PEG6-N<sub>3</sub>) incubated with 0841-DBCO at indicated molar ratios. F) SEC chromatograms of N<sub>3</sub>NLPs incubated with 0841 (lacking DBCO reactive group) at indicated molar ratios.</p>", "links"=>[], "tags"=>["biophysics", "biotechnology", "biomaterials", "Bionanotechnology", "immunology", "nanotechnology", "Clinical medicine", "pharmacology", "Drug research and development", "drug discovery", "pharmacokinetics", "Drug distribution", "Materials Science", "conjugation"], "article_id"=>976903, "categories"=>["Biological Sciences"], "users"=>["Nicholas O. Fischer", "Dina R. Weilhammer", "Alexis Dunkle", "Cynthia Thomas", "Mona Hwang", "Michele Corzett", "Cheri Lychak", "Wasima Mayer", "Salustra Urbin", "Nicole Collette", "Jiun Chiun Chang", "Gabriela G. Loots", "Amy Rasley", "Craig D. Blanchette"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093342.g005", "stats"=>{"downloads"=>3, "page_views"=>14, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Covalent_conjugation_of_protein_0841_to_the_N_3_NLPs_platform_/976903", "title"=>"Covalent conjugation of protein (0841) to the N<sub>3</sub>NLPs platform.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-27 03:27:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1438342"], "description"=>"<p>A) SEC analysis of the cODN∶NLP constructs at indicated cODN∶NLP molar ratios, monitored at 280 nm. The increase in absorbance at 280 nm and peak shift indicate successful incorporation of cODN. B) UV-Vis absorption spectra of SEC-purified cODN∶NLP (blue line) and apoE422k concentration matched NLP lacking the cODN (black line). The dashed lines represent the absorbance at 260 nm and 280 nm. C) Analysis of cODN incorporation into the NLP. The x-axis represents the cODN∶NLP ratio used during the NLP assembly reaction and the y-axis is the measured amount of cODN ultimately incorporated into the particle. D) SEC chromatograms of the PF∶NLP constructs at increasing PF-to-NLP ratios. E) UV-vis spectra of PF∶NLPs (blue line) and apoE422k concentration matched NLPs lacking the PF (black line). The dashed lines represent the absorbance at 280 nm and 368 nm. F) Analysis of PF incorporation efficiency into the NLP, represented as a function of the PF-to-NLP assembly ratio (x-axis) vs. measured PF-to-NLP ratio after purification (y-axis).</p>", "links"=>[], "tags"=>["biophysics", "biotechnology", "biomaterials", "Bionanotechnology", "immunology", "nanotechnology", "Clinical medicine", "pharmacology", "Drug research and development", "drug discovery", "pharmacokinetics", "Drug distribution", "Materials Science", "codn", "pf", "nlp"], "article_id"=>976904, "categories"=>["Biological Sciences"], "users"=>["Nicholas O. Fischer", "Dina R. Weilhammer", "Alexis Dunkle", "Cynthia Thomas", "Mona Hwang", "Michele Corzett", "Cheri Lychak", "Wasima Mayer", "Salustra Urbin", "Nicole Collette", "Jiun Chiun Chang", "Gabriela G. Loots", "Amy Rasley", "Craig D. Blanchette"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093342.g006", "stats"=>{"downloads"=>1, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Conjugation_of_cODN_and_PF_to_the_NLP_platform_/976904", "title"=>"Conjugation of cODN and PF to the NLP platform.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-27 03:27:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1438355"], "description"=>"<p>NiNLPs were administered by A) i.p. or B) i.n. routes and were assessed over 72 or 96 hours, respectively. Organ fluorescence was determined <i>ex vivo</i> and normalized to total organ weight. The normalized fluorescent intensity was quantitatively measured as a function of time. Data represent the average normalized fluorescence from groups of two animals, with standard error bars.</p>", "links"=>[], "tags"=>["biophysics", "biotechnology", "biomaterials", "Bionanotechnology", "immunology", "nanotechnology", "Clinical medicine", "pharmacology", "Drug research and development", "drug discovery", "pharmacokinetics", "Drug distribution", "Materials Science", "ninlp", "biodistribution"], "article_id"=>976917, "categories"=>["Biological Sciences"], "users"=>["Nicholas O. Fischer", "Dina R. Weilhammer", "Alexis Dunkle", "Cynthia Thomas", "Mona Hwang", "Michele Corzett", "Cheri Lychak", "Wasima Mayer", "Salustra Urbin", "Nicole Collette", "Jiun Chiun Chang", "Gabriela G. Loots", "Amy Rasley", "Craig D. Blanchette"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093342.g013", "stats"=>{"downloads"=>3, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Time_dependent_in_vivo_NiNLP_biodistribution_upon_i_p_and_i_n_administration_/976917", "title"=>"Time-dependent <i>in vivo</i> NiNLP biodistribution upon i.p. and i.n. administration.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-27 03:27:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1438340"], "description"=>"<p>A) SEC traces of NiNLPs incubated with His-tagged 0841 at indicated molar ratios. B) The 0841:NiNLPs were purified by SEC, and SDS-PAGE and densitometry were used to quantify the amount of 0841 and apoE422k protein in each NiNLP sample using known standards for each protein. These measured concentrations were used to calculate the number of proteins bound per NLP and were plotted as a function of the initial ratio used in the conjugation reaction.</p>", "links"=>[], "tags"=>["biophysics", "biotechnology", "biomaterials", "Bionanotechnology", "immunology", "nanotechnology", "Clinical medicine", "pharmacology", "Drug research and development", "drug discovery", "pharmacokinetics", "Drug distribution", "Materials Science", "conjugation", "ninlp"], "article_id"=>976902, "categories"=>["Biological Sciences"], "users"=>["Nicholas O. Fischer", "Dina R. Weilhammer", "Alexis Dunkle", "Cynthia Thomas", "Mona Hwang", "Michele Corzett", "Cheri Lychak", "Wasima Mayer", "Salustra Urbin", "Nicole Collette", "Jiun Chiun Chang", "Gabriela G. Loots", "Amy Rasley", "Craig D. Blanchette"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093342.g004", "stats"=>{"downloads"=>1, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Non_covalent_conjugation_of_protein_0841_to_the_NiNLP_platform_/976902", "title"=>"Non-covalent conjugation of protein (0841) to the NiNLP platform.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-27 03:27:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1438351"], "description"=>"<p>Groups of 10 female BALB/c mice were inoculated either i.n. or i.p. with NiNLP. As a positive control, a group of mice was injected with a known immunogenic recombinant subunit antigen (LcrV) co-administered with adjuvant (CpG). Serum IgG antibody titers against the scaffold protein, apoE422k (NiNLP-ip and NiNLP-in), or LcrV (LcrV+CpG-ip and LcrV+CpG-in) were assessed 4 weeks post-immunization. Each data point represents the titer value of an individual mouse.</p>", "links"=>[], "tags"=>["biophysics", "biotechnology", "biomaterials", "Bionanotechnology", "immunology", "nanotechnology", "Clinical medicine", "pharmacology", "Drug research and development", "drug discovery", "pharmacokinetics", "Drug distribution", "Materials Science", "ninlp"], "article_id"=>976913, "categories"=>["Biological Sciences"], "users"=>["Nicholas O. Fischer", "Dina R. Weilhammer", "Alexis Dunkle", "Cynthia Thomas", "Mona Hwang", "Michele Corzett", "Cheri Lychak", "Wasima Mayer", "Salustra Urbin", "Nicole Collette", "Jiun Chiun Chang", "Gabriela G. Loots", "Amy Rasley", "Craig D. Blanchette"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093342.g011", "stats"=>{"downloads"=>2, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Assessment_of_NiNLP_immunogenicity_/976913", "title"=>"Assessment of NiNLP immunogenicity.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-27 03:27:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1438336"], "description"=>"<p>The indicated peaks correspond to intact NLP (t<sub>R</sub> 9.5 min) and free apoE422k scaffold protein (t<sub>R</sub> 12.2 min). Absorbance of AF647-labeled apoE422k absorbance was monitored at 600 nm.</p>", "links"=>[], "tags"=>["biophysics", "biotechnology", "biomaterials", "Bionanotechnology", "immunology", "nanotechnology", "Clinical medicine", "pharmacology", "Drug research and development", "drug discovery", "pharmacokinetics", "Drug distribution", "Materials Science", "serum", "sec", "24", "hrs"], "article_id"=>976898, "categories"=>["Biological Sciences"], "users"=>["Nicholas O. Fischer", "Dina R. Weilhammer", "Alexis Dunkle", "Cynthia Thomas", "Mona Hwang", "Michele Corzett", "Cheri Lychak", "Wasima Mayer", "Salustra Urbin", "Nicole Collette", "Jiun Chiun Chang", "Gabriela G. Loots", "Amy Rasley", "Craig D. Blanchette"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093342.g001", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Stability_of_DMPC_8758_NLPs_in_20_serum_assessed_by_SEC_over_24_hrs_at_25_176_C_/976898", "title"=>"Stability of DMPC∶NLPs in 20% serum assessed by SEC over 24 hrs at 25°C.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-27 03:27:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1438349"], "description"=>"<p>H&E stained sections of livers obtained from mice that had received 25 µg of NiNLP i.n. (30 µl) or i.p. (100 µl) daily for 14 consecutive days at 10×, 20× and 40× magnifications.</p>", "links"=>[], "tags"=>["biophysics", "biotechnology", "biomaterials", "Bionanotechnology", "immunology", "nanotechnology", "Clinical medicine", "pharmacology", "Drug research and development", "drug discovery", "pharmacokinetics", "Drug distribution", "Materials Science", "repeated", "ninlp", "microstructure"], "article_id"=>976911, "categories"=>["Biological Sciences"], "users"=>["Nicholas O. Fischer", "Dina R. Weilhammer", "Alexis Dunkle", "Cynthia Thomas", "Mona Hwang", "Michele Corzett", "Cheri Lychak", "Wasima Mayer", "Salustra Urbin", "Nicole Collette", "Jiun Chiun Chang", "Gabriela G. Loots", "Amy Rasley", "Craig D. Blanchette"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093342.g010", "stats"=>{"downloads"=>1, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Histological_analysis_of_the_effect_of_repeated_NiNLP_administration_on_the_microstructure_of_the_liver_/976911", "title"=>"Histological analysis of the effect of repeated NiNLP administration on the microstructure of the liver.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-27 03:27:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1438345"], "description"=>"<p>Weights of A) male and B) female mice receiving daily NiNLP injections i.n. (30 µl) or i.p. (100 µl) for 14 consecutive days. Control mice received equal volumes of PBS i.p. over the same 14-day time course. Data represent averaged weights from groups of three animals, with standard deviation error bars.</p>", "links"=>[], "tags"=>["biophysics", "biotechnology", "biomaterials", "Bionanotechnology", "immunology", "nanotechnology", "Clinical medicine", "pharmacology", "Drug research and development", "drug discovery", "pharmacokinetics", "Drug distribution", "Materials Science", "repeated", "ninlp"], "article_id"=>976907, "categories"=>["Biological Sciences"], "users"=>["Nicholas O. Fischer", "Dina R. Weilhammer", "Alexis Dunkle", "Cynthia Thomas", "Mona Hwang", "Michele Corzett", "Cheri Lychak", "Wasima Mayer", "Salustra Urbin", "Nicole Collette", "Jiun Chiun Chang", "Gabriela G. Loots", "Amy Rasley", "Craig D. Blanchette"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093342.g008", "stats"=>{"downloads"=>1, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_repeated_NiNLP_administration_on_mouse_body_weights_/976907", "title"=>"Effect of repeated NiNLP administration on mouse body weights.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-27 03:27:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1438344"], "description"=>"<p>A) NiNLP cytotoxicity was measured using the MTT assay with UMR cells at NLP treatment doses ranging from 6–50 µg/ml (cell viability values are relative to the PBS treated cells). Toxicity of the NiNLPs measured using a LDH release assay with B) J774 cells at NLP treatment doses ranging from 12–52 µg/ml, C) Hep G2 cells at NiNLP treatment doses ranging from 5–320 µg/ml, and D) primary murine immune cells (macrophages, DCs, NKs, B cells, and T cells) at an NiNLP dose of 25 µg/ml. All values in the LDH release studies were normalized to the PBS treated cells. Lysed cells were used as a positive control. Results are shown as mean values from duplicate experiments, with error bars representing standard deviation values.</p>", "links"=>[], "tags"=>["biophysics", "biotechnology", "biomaterials", "Bionanotechnology", "immunology", "nanotechnology", "Clinical medicine", "pharmacology", "Drug research and development", "drug discovery", "pharmacokinetics", "Drug distribution", "Materials Science", "vitro", "cytotoxicity", "ninlp"], "article_id"=>976906, "categories"=>["Biological Sciences"], "users"=>["Nicholas O. Fischer", "Dina R. Weilhammer", "Alexis Dunkle", "Cynthia Thomas", "Mona Hwang", "Michele Corzett", "Cheri Lychak", "Wasima Mayer", "Salustra Urbin", "Nicole Collette", "Jiun Chiun Chang", "Gabriela G. Loots", "Amy Rasley", "Craig D. Blanchette"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093342.g007", "stats"=>{"downloads"=>2, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_In_vitro_cytotoxicity_of_the_NiNLP_platform_/976906", "title"=>"In vitro cytotoxicity of the NiNLP platform.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-27 03:27:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1438339"], "description"=>"<p>Conjugation to NLPs can be achieved using A) lipids bearing functional head groups (e.g. chelated Ni or azide moieties) to which cargo molecules with corresponding functionality can be conjugated (His-tagged or alkyne-functionalized proteins, respectively), B) biomolecules featuring lipidic moieties (e.g. cholesterol or alkyl chains) which can anchor the molecule into the lipid bilayer, or C) orthogonal combination of the two strategies.</p>", "links"=>[], "tags"=>["biophysics", "biotechnology", "biomaterials", "Bionanotechnology", "immunology", "nanotechnology", "Clinical medicine", "pharmacology", "Drug research and development", "drug discovery", "pharmacokinetics", "Drug distribution", "Materials Science", "strategies", "conjugating", "therapeutic", "molecules", "nlp"], "article_id"=>976901, "categories"=>["Biological Sciences"], "users"=>["Nicholas O. Fischer", "Dina R. Weilhammer", "Alexis Dunkle", "Cynthia Thomas", "Mona Hwang", "Michele Corzett", "Cheri Lychak", "Wasima Mayer", "Salustra Urbin", "Nicole Collette", "Jiun Chiun Chang", "Gabriela G. Loots", "Amy Rasley", "Craig D. Blanchette"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093342.g003", "stats"=>{"downloads"=>1, "page_views"=>17, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Illustration_of_strategies_for_conjugating_biological_or_therapeutic_cargo_molecules_to_the_NLP_platform_/976901", "title"=>"Illustration of strategies for conjugating biological or therapeutic cargo molecules to the NLP platform.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-27 03:27:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1438338"], "description"=>"<p>Integrated NLP peak area of the SEC chromatograms for A) DOPC∶NLPs incubated at 25°C and B) DMPC∶NLPs incubated at 25°C. C) t<sub>1/2</sub> of the DOPC∶NLPs (blue line) and DMPC∶NLPs (red line) incubated at 25°C. Integrated NLP peak area of the SEC chromatograms for D) DOPC∶NLPs incubated at 37°C and E) DMPC∶NLPs incubated at 37°C. F) t<sub>1/2</sub> of the DOPC∶NLPs (blue line) and DMPC∶NLPs (red line) incubated at 37°C. AF647-labeled apoE422k absorbance was monitored at 600 nm.</p>", "links"=>[], "tags"=>["biophysics", "biotechnology", "biomaterials", "Bionanotechnology", "immunology", "nanotechnology", "Clinical medicine", "pharmacology", "Drug research and development", "drug discovery", "pharmacokinetics", "Drug distribution", "Materials Science", "nlps", "lipid", "serum"], "article_id"=>976900, "categories"=>["Biological Sciences"], "users"=>["Nicholas O. Fischer", "Dina R. Weilhammer", "Alexis Dunkle", "Cynthia Thomas", "Mona Hwang", "Michele Corzett", "Cheri Lychak", "Wasima Mayer", "Salustra Urbin", "Nicole Collette", "Jiun Chiun Chang", "Gabriela G. Loots", "Amy Rasley", "Craig D. Blanchette"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093342.g002", "stats"=>{"downloads"=>0, "page_views"=>24, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Stability_of_NLPs_as_a_function_of_lipid_content_temperature_time_and_serum_concentration_/976900", "title"=>"Stability of NLPs as a function of lipid content, temperature, time, and serum concentration.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-27 03:27:38"}
  • {"files"=>["https://ndownloader.figshare.com/files/1438352"], "description"=>"<p>A) Fluorescent images of the kidney, liver, spleen and lung 2 hrs after i.p. and i.n administration of AF750-labeled NiNLPs. Groups of 3 mice were injected B) i.v., C) i.p., D) i.m., E) s.c. or F) i.n. with AF750-labeled NiNLPs. Organs were excised 2, 4, and 24 hrs post-injection and fluorescence intensity quantified as a function of time and normalized to total organ weight. Data represent the average normalized fluorescence from groups of three animals, with standard error bars.</p>", "links"=>[], "tags"=>["biophysics", "biotechnology", "biomaterials", "Bionanotechnology", "immunology", "nanotechnology", "Clinical medicine", "pharmacology", "Drug research and development", "drug discovery", "pharmacokinetics", "Drug distribution", "Materials Science", "biodistribution"], "article_id"=>976914, "categories"=>["Biological Sciences"], "users"=>["Nicholas O. Fischer", "Dina R. Weilhammer", "Alexis Dunkle", "Cynthia Thomas", "Mona Hwang", "Michele Corzett", "Cheri Lychak", "Wasima Mayer", "Salustra Urbin", "Nicole Collette", "Jiun Chiun Chang", "Gabriela G. Loots", "Amy Rasley", "Craig D. Blanchette"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093342.g012", "stats"=>{"downloads"=>1, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_In_vivo_biodistribution_of_the_NiNLP_/976914", "title"=>"<i>In vivo</i> biodistribution of the NiNLP.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-27 03:27:38"}

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