Drosophila Mcm10 Is Required for DNA Replication and Differentiation in the Compound Eye
Events
Loading … Spinner

Mendeley | Further Information

{"title"=>"Drosophila Mcm10 is required for DNA replication and differentiation in the compound eye", "type"=>"journal", "authors"=>[{"first_name"=>"Nicole", "last_name"=>"Vo", "scopus_author_id"=>"55486837900"}, {"first_name"=>"Ayano", "last_name"=>"Taga", "scopus_author_id"=>"56150112300"}, {"first_name"=>"Yasuhiro", "last_name"=>"Inaba", "scopus_author_id"=>"56149027100"}, {"first_name"=>"Hideki", "last_name"=>"Yoshida", "scopus_author_id"=>"55731764400"}, {"first_name"=>"Sue", "last_name"=>"Cotterill", "scopus_author_id"=>"7006021766"}, {"first_name"=>"Masamitsu", "last_name"=>"Yamaguchi", "scopus_author_id"=>"35473744200"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"isbn"=>"1932-6203 (Electronic)\\r1932-6203 (Linking)", "doi"=>"10.1371/journal.pone.0093450", "sgr"=>"84899868034", "issn"=>"19326203", "scopus"=>"2-s2.0-84899868034", "pui"=>"373031069", "pmid"=>"24686397"}, "id"=>"b6d6b23a-31c6-3b81-a331-9c15480d4b45", "abstract"=>"Mini chromosome maintenance 10 (Mcm10) is an essential protein, which is conserved from S. cerevisiae to Drosophila and human, and is required for the initiation of DNA replication. Knockdown of Drosophila Mcm10 (dMcm10) by RNA interference in eye imaginal discs induces abnormal eye morphology (rough eye phenotype), and the number of ommatidia is decreased in adult eyes. We also observed a delay in the S phase and M phase in eye discs of dMcm10 knockdown fly lines. These results show important roles for dMcm10 in the progression of S and M phases. Furthermore, genome damage and apoptosis were induced by dMcm10 knockdown in eye imaginal discs. Surprisingly, when we used deadpan-lacZ and klingon-lacZ enhancer trap lines to monitor the photoreceptor cells in eye discs, knockdown of dMcm10 by the GMR-GAL4 driver reduced the signals of R7 photoreceptor cells. These data suggest an involvement of dMcm10 in R7 cell differentiation. This involvement appears to be independent of the apoptosis induced by dMcm10 knockdown. Together, these results suggest that dMcm10 knockdown has an effect on DNA replication and R7 cell differentiation.", "link"=>"http://www.mendeley.com/research/drosophila-mcm10-required-dna-replication-differentiation-compound-eye", "reader_count"=>8, "reader_count_by_academic_status"=>{"Researcher"=>2, "Student > Ph. D. Student"=>5, "Unspecified"=>1}, "reader_count_by_user_role"=>{"Researcher"=>2, "Student > Ph. D. Student"=>5, "Unspecified"=>1}, "reader_count_by_subject_area"=>{"Environmental Science"=>1, "Agricultural and Biological Sciences"=>6, "Unspecified"=>1}, "reader_count_by_subdiscipline"=>{"Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>6}, "Environmental Science"=>{"Environmental Science"=>1}, "Unspecified"=>{"Unspecified"=>1}}, "reader_count_by_country"=>{"Turkey"=>1}, "group_count"=>0}

Scopus | Further Information

{"@_fa"=>"true", "link"=>[{"@_fa"=>"true", "@ref"=>"self", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84899868034"}, {"@_fa"=>"true", "@ref"=>"author-affiliation", "@href"=>"https://api.elsevier.com/content/abstract/scopus_id/84899868034?field=author,affiliation"}, {"@_fa"=>"true", "@ref"=>"scopus", "@href"=>"https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84899868034&origin=inward"}, {"@_fa"=>"true", "@ref"=>"scopus-citedby", "@href"=>"https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84899868034&origin=inward"}], "prism:url"=>"https://api.elsevier.com/content/abstract/scopus_id/84899868034", "dc:identifier"=>"SCOPUS_ID:84899868034", "eid"=>"2-s2.0-84899868034", "dc:title"=>"Drosophila Mcm10 is required for DNA replication and differentiation in the compound eye", "dc:creator"=>"Vo N.", "prism:publicationName"=>"PLoS ONE", "prism:eIssn"=>"19326203", "prism:volume"=>"9", "prism:issueIdentifier"=>"3", "prism:pageRange"=>nil, "prism:coverDate"=>"2014-03-31", "prism:coverDisplayDate"=>"31 March 2014", "prism:doi"=>"10.1371/journal.pone.0093450", "citedby-count"=>"5", "affiliation"=>[{"@_fa"=>"true", "affilname"=>"Kyoto Institute of Technology", "affiliation-city"=>"Kyoto", "affiliation-country"=>"Japan"}], "pubmed-id"=>"24686397", "prism:aggregationType"=>"Journal", "subtype"=>"ar", "subtypeDescription"=>"Article", "article-number"=>"e93450", "source-id"=>"10600153309", "openaccess"=>"1", "openaccessFlag"=>true}

Facebook

  • {"url"=>"http%3A%2F%2Fjournals.plos.org%2Fplosone%2Farticle%3Fid%3D10.1371%252Fjournal.pone.0093450", "share_count"=>0, "like_count"=>0, "comment_count"=>0, "click_count"=>0, "total_count"=>0}

Counter

  • {"month"=>"3", "year"=>"2014", "pdf_views"=>"26", "xml_views"=>"17", "html_views"=>"194"}
  • {"month"=>"4", "year"=>"2014", "pdf_views"=>"56", "xml_views"=>"12", "html_views"=>"277"}
  • {"month"=>"5", "year"=>"2014", "pdf_views"=>"13", "xml_views"=>"2", "html_views"=>"102"}
  • {"month"=>"6", "year"=>"2014", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"93"}
  • {"month"=>"7", "year"=>"2014", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"86"}
  • {"month"=>"8", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"2", "html_views"=>"40"}
  • {"month"=>"9", "year"=>"2014", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"45"}
  • {"month"=>"10", "year"=>"2014", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"32"}
  • {"month"=>"11", "year"=>"2014", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"29"}
  • {"month"=>"12", "year"=>"2014", "pdf_views"=>"9", "xml_views"=>"2", "html_views"=>"43"}
  • {"month"=>"1", "year"=>"2015", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"44"}
  • {"month"=>"2", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"15"}
  • {"month"=>"3", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"4", "year"=>"2015", "pdf_views"=>"7", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"5", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"16"}
  • {"month"=>"6", "year"=>"2015", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"7", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"10"}
  • {"month"=>"8", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"13"}
  • {"month"=>"9", "year"=>"2015", "pdf_views"=>"2", "xml_views"=>"1", "html_views"=>"31"}
  • {"month"=>"10", "year"=>"2015", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"17"}
  • {"month"=>"11", "year"=>"2015", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"18"}
  • {"month"=>"12", "year"=>"2015", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"1", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"25"}
  • {"month"=>"2", "year"=>"2016", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"3", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"38"}
  • {"month"=>"4", "year"=>"2016", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"29"}
  • {"month"=>"5", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"6", "year"=>"2016", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"9"}
  • {"month"=>"7", "year"=>"2016", "pdf_views"=>"10", "xml_views"=>"0", "html_views"=>"14"}
  • {"month"=>"8", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"8"}
  • {"month"=>"9", "year"=>"2016", "pdf_views"=>"6", "xml_views"=>"0", "html_views"=>"24"}
  • {"month"=>"10", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"19"}
  • {"month"=>"11", "year"=>"2016", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"12", "year"=>"2016", "pdf_views"=>"9", "xml_views"=>"1", "html_views"=>"19"}
  • {"month"=>"1", "year"=>"2017", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"20"}
  • {"month"=>"2", "year"=>"2017", "pdf_views"=>"15", "xml_views"=>"0", "html_views"=>"33"}
  • {"month"=>"3", "year"=>"2017", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"27"}
  • {"month"=>"4", "year"=>"2017", "pdf_views"=>"17", "xml_views"=>"0", "html_views"=>"23"}
  • {"month"=>"5", "year"=>"2017", "pdf_views"=>"16", "xml_views"=>"0", "html_views"=>"11"}
  • {"month"=>"6", "year"=>"2017", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"17"}
  • {"month"=>"7", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"0", "html_views"=>"12"}
  • {"month"=>"8", "year"=>"2017", "pdf_views"=>"8", "xml_views"=>"1", "html_views"=>"6"}
  • {"month"=>"9", "year"=>"2017", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"10"}
  • {"month"=>"10", "year"=>"2017", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"22"}
  • {"month"=>"11", "year"=>"2017", "pdf_views"=>"11", "xml_views"=>"2", "html_views"=>"49"}
  • {"month"=>"12", "year"=>"2017", "pdf_views"=>"1", "xml_views"=>"2", "html_views"=>"25"}
  • {"month"=>"1", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"14"}
  • {"month"=>"2", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"3", "year"=>"2018", "pdf_views"=>"9", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"4", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"5", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"1", "html_views"=>"2"}
  • {"month"=>"6", "year"=>"2018", "pdf_views"=>"5", "xml_views"=>"1", "html_views"=>"3"}
  • {"month"=>"7", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"3", "html_views"=>"2"}
  • {"month"=>"8", "year"=>"2018", "pdf_views"=>"1", "xml_views"=>"1", "html_views"=>"1"}
  • {"month"=>"9", "year"=>"2018", "pdf_views"=>"4", "xml_views"=>"1", "html_views"=>"4"}
  • {"month"=>"10", "year"=>"2018", "pdf_views"=>"0", "xml_views"=>"1", "html_views"=>"0"}
  • {"month"=>"11", "year"=>"2018", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"12", "year"=>"2018", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"7"}
  • {"month"=>"1", "year"=>"2019", "pdf_views"=>"3", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"2", "year"=>"2019", "pdf_views"=>"5", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"3", "year"=>"2019", "pdf_views"=>"7", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"4", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"2"}
  • {"month"=>"5", "year"=>"2019", "pdf_views"=>"0", "xml_views"=>"0", "html_views"=>"3"}
  • {"month"=>"6", "year"=>"2019", "pdf_views"=>"1", "xml_views"=>"0", "html_views"=>"0"}
  • {"month"=>"7", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"1"}
  • {"month"=>"8", "year"=>"2019", "pdf_views"=>"2", "xml_views"=>"0", "html_views"=>"3"}

Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1440944"], "description"=>"<p>The eye imaginal discs were stained with anti-Ser10 phosphorylated histone H3 (PH3) antibody (Green) (A, B, C). Also shown are merged images of the eye discs stained with DAPI for DNA (Blue) and anti PH3 antibody (Green) (D, E, F). (A, D) <i>GMR</i>-GAL4/+; +; +; (B, E) <i>GMR</i>-GAL4/+; +; UAS-<i>Mcm10IR<sub>633-700</sub></i>/+; (C, F) <i>GMR</i>-GAL4/+; +; UAS-<i>Mcm10IR<sub>633-700</sub></i>/UAS-<i>Mcm10IR<sub>633-700</sub></i>. The white arrowhead shows the morphogenetic furrow. The flies were reared at 28°C. Bars indicate 40 μm. (G) Quantification of phosphorylated histone H3 positive index in the posterior region of the eye discs. Flies carrying two copies of UAS-<i>dMcm10IR<sub>633-700</sub></i> caused a significant increase in the number of mitotic cells in the posterior region compared to control flies carrying <i>GMR</i>-GAL4 alone. Flies carrying one copy of UAS-<i>dMcm10IR<sub>633-700</sub></i> also caused a delay in M phase, but not as much as with two copies. **p<0.01, ****p<0.0001. The red border line indicates the posterior region. (a) indicates anterior, (p) indicates posterior.</p>", "links"=>[], "tags"=>["Biochemistry", "dna", "DNA replication", "Nucleic acids", "developmental biology", "genetics", "Molecular genetics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "drosophila", "Drosophila melanogaster", "Model organisms", "Animal models", "imaginal", "discs"], "article_id"=>979072, "categories"=>["Biological Sciences"], "users"=>["Nicole Vo", "Ayano Taga", "Yasuhiro Inaba", "Hideki Yoshida", "Sue Cotterill", "Masamitsu Yamaguchi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093450.g005", "stats"=>{"downloads"=>1, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Knockdown_of_dMcm10_in_eye_imaginal_discs_induces_a_delay_in_M_phase_/979072", "title"=>"Knockdown of <i>dMcm10</i> in eye imaginal discs induces a delay in M phase.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-31 02:47:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1440994"], "description"=>"<p>The eye imaginal discs of third instar lavae were stained with anti-Prospero antibody (Green) (A, D, G). Merged images were generated of eye discs staining with anti-Prospero antibody (Green) and anti-cleaved caspase-3 antibody (Red) (B, E, H). Images of eye discs staining DNA with DAPI (Blue) (C, F, I) are also shown. (A, B, C) shows <i>GMR</i>-GAL4/<i>yw</i>; +; +; (D, E, F) shows <i>GMR</i>-GAL4/<i>yw</i>; +; UAS-d<i>Mcm10IR<sub>633-700</sub></i>/+; and (G, H, J) shows <i>GMR</i>-GAL4/<i>yw</i>; +; UAS-d<i>Mcm10IR<sub>633-700</sub></i>/UAS-<i>P35</i>. White arrowhead shows morphogenetic furrow. Bars indicate 40 μm. The flies were reared at 28°C.</p>", "links"=>[], "tags"=>["Biochemistry", "dna", "DNA replication", "Nucleic acids", "developmental biology", "genetics", "Molecular genetics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "drosophila", "Drosophila melanogaster", "Model organisms", "Animal models", "imaginal", "discs", "inhibits", "r7", "photoreceptor", "differentiation", "independently", "apoptotic"], "article_id"=>979102, "categories"=>["Biological Sciences"], "users"=>["Nicole Vo", "Ayano Taga", "Yasuhiro Inaba", "Hideki Yoshida", "Sue Cotterill", "Masamitsu Yamaguchi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093450.g010", "stats"=>{"downloads"=>1, "page_views"=>20, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Knockdown_of_dMcm10_in_eye_imaginal_discs_inhibits_R7_photoreceptor_cell_differentiation_independently_of_its_apoptotic_effect_/979102", "title"=>"Knockdown of <i>dMcm10</i> in eye imaginal discs inhibits R7 photoreceptor cell differentiation independently of its apoptotic effect.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-31 02:47:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1441004", "https://ndownloader.figshare.com/files/1441005", "https://ndownloader.figshare.com/files/1441006"], "description"=>"<div><p>Mini chromosome maintenance 10 (Mcm10) is an essential protein, which is conserved from <i>S. cerevisiae</i> to <i>Drosophila</i> and human, and is required for the initiation of DNA replication. Knockdown of <i>Drosophila Mcm10</i> (<i>dMcm10)</i> by RNA interference in eye imaginal discs induces abnormal eye morphology (rough eye phenotype), and the number of ommatidia is decreased in adult eyes. We also observed a delay in the S phase and M phase in eye discs of <i>dMcm10</i> knockdown fly lines. These results show important roles for dMcm10 in the progression of S and M phases. Furthermore, genome damage and apoptosis were induced by <i>dMcm10</i> knockdown in eye imaginal discs. Surprisingly, when we used <i>deadpan-lacZ</i> and <i>klingon-lacZ</i> enhancer trap lines to monitor the photoreceptor cells in eye discs, knockdown of <i>dMcm10</i> by the <i>GMR</i>-GAL4 driver reduced the signals of R7 photoreceptor cells. These data suggest an involvement of dMcm10 in R7 cell differentiation. This involvement appears to be independent of the apoptosis induced by <i>dMcm10</i> knockdown. Together, these results suggest that <i>dMcm10</i> knockdown has an effect on DNA replication and R7 cell differentiation.</p></div>", "links"=>[], "tags"=>["Biochemistry", "dna", "DNA replication", "Nucleic acids", "developmental biology", "genetics", "Molecular genetics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "drosophila", "Drosophila melanogaster", "Model organisms", "Animal models", "mcm10", "replication", "differentiation"], "article_id"=>979112, "categories"=>["Biological Sciences"], "users"=>["Nicole Vo", "Ayano Taga", "Yasuhiro Inaba", "Hideki Yoshida", "Sue Cotterill", "Masamitsu Yamaguchi"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0093450.s001", "https://dx.doi.org/10.1371/journal.pone.0093450.s002", "https://dx.doi.org/10.1371/journal.pone.0093450.s003"], "stats"=>{"downloads"=>0, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Drosophila_Mcm10_Is_Required_for_DNA_Replication_and_Differentiation_in_the_Compound_Eye/979112", "title"=>"<i>Drosophila</i> Mcm10 Is Required for DNA Replication and Differentiation in the Compound Eye", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-03-31 02:47:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1440927"], "description"=>"<p>In the flip out experiment: (A) Eye imaginal discs of Canton S are stained with anti-dMcm10 antibody (Red). (B) Eye discs expressing <i>dMcm10</i> dsRNA are stained with anti-dMcm10 antibody (Red). (C) Cells expressing <i>dMcm10</i> dsRNA are marked with GFP (Green). (D) Merged image of anti-dMcm10 and GFP signals in <i>dMcm10</i> knockdown eye discs. The white border lines show the GFP-negative region in which <i>dMcm10</i> dsRNA is not expressed. The red border line shows the region in which the eye disc is folded back and some apparently overlapped signals in this region. In the experiment for <i>dMcm10</i> knockdown by <i>GMR</i>-GAL4 driver: (E) <i>GMR</i>-GAL4: +: +; (F) <i>w</i>; +; UAS-<i>Mcm10IR<sub>633-700</sub></i>, (G) <i>w</i>; UAS-<i>Mcm10IR<sub>3-117</sub></i>; +; (H) <i>GMR</i>-GAL4; +; UAS-<i>Mcm10IR<sub>633-700</sub></i>; (I) <i>GMR</i>-GAL4: UAS<i>-Mcm10IR<sub>3-117</sub></i>; +. (E-I) eye imaginal discs are stained with anti-dMcm10 antibody (Green). (E–G) dMcm10 signals are observed everywhere in the eye discs including the posterior regions (white border line). However, in two independent knockdown flies (H and I), there is a significant reduction of dMcm10 signals in the posterior regions. White arrowheads indicate morphogenetic furrow (MF). The bars indicate 50 μm and 40 μm respectively. (a) indicates anterior, (p) indicates posterior.</p>", "links"=>[], "tags"=>["Biochemistry", "dna", "DNA replication", "Nucleic acids", "developmental biology", "genetics", "Molecular genetics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "drosophila", "Drosophila melanogaster", "Model organisms", "Animal models", "dsrna", "dmcm10"], "article_id"=>979056, "categories"=>["Biological Sciences"], "users"=>["Nicole Vo", "Ayano Taga", "Yasuhiro Inaba", "Hideki Yoshida", "Sue Cotterill", "Masamitsu Yamaguchi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093450.g003", "stats"=>{"downloads"=>0, "page_views"=>1, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_of_dMcm10_dsRNA_reduces_dMcm10_levels_in_eye_discs_/979056", "title"=>"Expression of <i>dMcm10</i> dsRNA reduces dMcm10 levels in eye discs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-31 02:47:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1440936"], "description"=>"<p>The eye imaginal discs were labeled with EdU (Red) (A) <i>GMR</i>-GAL4/+; +; +; (B) <i>GMR</i>-GAL4/+; +; UAS-<i>Mcm10IR<sub>633-700</sub></i>/+; (C) <i>GMR</i>-GAL4/+; +; UAS-<i>Mcm10IR<sub>633-700</sub></i>/UAS-<i>Mcm10IR<sub>633-700</sub></i>. The arrows show the positions measured. (D) Quantification of the width of S phase zone in the posterior region. ***p<0.001, ***p<0.0001. The yellow square shows the position of an EdU cell possibly undergoing replication of the late replicating heterochromatic region. The blue square shows the position of an EdU cell possibly undergoing replication of the early replicating euchromatic region. Figure E–G present higher magnification images of the blue square. Figure H–J present higher magnification images of the yellow square. Cells were stained with EdU (Red) (E and H) or Hoechst 33342 (Blue) (F and I). Figures G and J are merged images of both EdU and Hoechst 33342. The bars indicate 10 mm. The flies were reared at 28°C. White arrowheads indicate morphogenetic furrow (MF). (a) indicates anterior, (p) indicates posterior.</p>", "links"=>[], "tags"=>["Biochemistry", "dna", "DNA replication", "Nucleic acids", "developmental biology", "genetics", "Molecular genetics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "drosophila", "Drosophila melanogaster", "Model organisms", "Animal models", "imaginal", "discs"], "article_id"=>979065, "categories"=>["Biological Sciences"], "users"=>["Nicole Vo", "Ayano Taga", "Yasuhiro Inaba", "Hideki Yoshida", "Sue Cotterill", "Masamitsu Yamaguchi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093450.g004", "stats"=>{"downloads"=>0, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Knockdown_of_dMcm10_in_eye_imaginal_discs_induces_a_delay_in_S_phase_/979065", "title"=>"Knockdown of <i>dMcm10</i> in eye imaginal discs induces a delay in S phase.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-31 02:47:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1440971"], "description"=>"<p>The eye imaginal discs of third instar lavae were stained with anti-cleaved caspase-3 antibody(A, B, C) (Red). The merged images of eye discs stained with DAPI for DNA (Blue) and anti-cleaved caspase-3 antibody (Red) (D, E, F). (A, D) <i>GMR</i>-GAL4/<i>yw</i>; +; +; (B, E) <i>GMR</i>-GAL4/<i>yw</i>; +; UAS-<i>Mcm10IR<sub>633-700</sub></i>/+; (C, F) <i>GMR</i>-GAL4/<i>yw</i>; +; UAS-<i>Mcm10IR<sub>633-700</sub></i>/UAS-<i>P35</i>. White arrowhead shows morphogenetic furrow. The flies were reared at 28°C. Bars indicate 40 μm.</p>", "links"=>[], "tags"=>["Biochemistry", "dna", "DNA replication", "Nucleic acids", "developmental biology", "genetics", "Molecular genetics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "drosophila", "Drosophila melanogaster", "Model organisms", "Animal models", "imaginal", "discs"], "article_id"=>979082, "categories"=>["Biological Sciences"], "users"=>["Nicole Vo", "Ayano Taga", "Yasuhiro Inaba", "Hideki Yoshida", "Sue Cotterill", "Masamitsu Yamaguchi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093450.g007", "stats"=>{"downloads"=>1, "page_views"=>9, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Knockdown_of_dMcm10_in_eye_imaginal_discs_induces_apoptosis_/979082", "title"=>"Knockdown of <i>dMcm10</i> in eye imaginal discs induces apoptosis.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-31 02:47:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1440985"], "description"=>"<p>Immunostaining of eye discs with anti-lacZ antibodies (Green) (A) <i>GMR</i>-GAL4/+; deadpan-lacZ/+; UAS-<i>GFPIR</i>/+; (B)<i>GMR</i>-GAL4/+; deadpan-lacZ/+; UAS-d<i>Mcm10IR<sub>633-700</sub></i>/+; (C) <i>GMR</i>-GAL4/+; deadpan-lacZ/UAS-d<i>Mcm10IR<sub>3-117</sub></i>, +; (D) <i>GMR</i>-GAL4/+; +; UAS-<i>GFPIR</i>/klingon-lacZ, (E) <i>GMR</i>-GAL4/+; +; UAS-<i>Mcm10IR<sub>633-700</sub></i>/klingon-lacZ; (F) <i>GMR</i>-GAL4/+; UAS-d<i>Mcm10IR<sub>3-117</sub></i>/+; klingon-lacZ/+. (A–C) R3, R4, and R7 photoreceptor cells are marked by P82 (deadpan-lacZ) and (D–F) R7 photoreceptor cells are marked by B38 (klingon-lacZ). R7 photoreceptor cells were reduced significantly in both of the knockdown lines: <i>dMcm10IR<sub>633-700</sub></i> (B, E) and <i>dMcm10IR<sub>3-117</sub></i> (C, F). Bars indicate 10 μm. The flies were reared at 28°C.</p>", "links"=>[], "tags"=>["Biochemistry", "dna", "DNA replication", "Nucleic acids", "developmental biology", "genetics", "Molecular genetics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "drosophila", "Drosophila melanogaster", "Model organisms", "Animal models", "knockdown", "photoreceptor", "cells", "monitored", "enhancer"], "article_id"=>979096, "categories"=>["Biological Sciences"], "users"=>["Nicole Vo", "Ayano Taga", "Yasuhiro Inaba", "Hideki Yoshida", "Sue Cotterill", "Masamitsu Yamaguchi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093450.g009", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Effect_of_dMcm10_knockdown_on_photoreceptor_cells_monitored_by_two_enhancer_trap_lines_/979096", "title"=>"Effect of <i>dMcm10</i> knockdown on photoreceptor cells monitored by two enhancer trap lines.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-31 02:47:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1440915"], "description"=>"<p>Scanning electron micrographs of adult compound eyes. Posterior is to the right and dorsal is to the top. The bar indicates 50 μm. (A) <i>w</i>; +; UAS-<i>Mcm10IR<sub>633-700</sub></i>; (B) <i>w</i>; UAS-<i>Mcm10IR<sub>3-117</sub></i>; +; (C) <i>GMR</i>-GAL4/+; +; +; (D) <i>GMR</i>-GAL4/+; +; UAS-<i>dMcm10IR<sub>633-700</sub></i>/+; (E) <i>GMR</i>-GAL4/+; +; UAS-<i>Mcm10IR<sub>633-700</sub></i>/UAS-<i>MCM10IR<sub>633-700</sub></i>; (F)<i>GMR</i>-GAL4/+;UAS-<i>Mcm10IR<sub>3-117</sub></i>/UAS-<i>Mcm10IR<sub>3-117</sub></i>;+; (G) Quantification of the number of ommatidium in adult eye flies. Mean intensities with standard deviation from six adult eyes are shown. ***p<0.001, ****p<0.0001. The flies were reared at 28°C.</p>", "links"=>[], "tags"=>["Biochemistry", "dna", "DNA replication", "Nucleic acids", "developmental biology", "genetics", "Molecular genetics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "drosophila", "Drosophila melanogaster", "Model organisms", "Animal models", "imaginal", "discs", "causes"], "article_id"=>979045, "categories"=>["Biological Sciences"], "users"=>["Nicole Vo", "Ayano Taga", "Yasuhiro Inaba", "Hideki Yoshida", "Sue Cotterill", "Masamitsu Yamaguchi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093450.g002", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Knockdown_of_dMcm10_in_eye_imaginal_discs_causes_a_small_and_rough_eye_phenotype_/979045", "title"=>"Knockdown of <i>dMcm10</i> in eye imaginal discs causes a small and rough eye phenotype.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-31 02:47:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1440980"], "description"=>"<p>Scanning electron micrographs of adult compound eyes. Posterior is to the right and dorsal is to the top. The flies were reared at 28°C. The bar indicates 50 μm. (A) <i>GMR</i>-GAL4/<i>yw</i>; +; +; (B) <i>GMR</i>-GAL4/<i>yw</i>; +; UAS-<i>Mcm10IR<sub>633-700</sub></i>/+; (C) <i>GMR</i>-GAL4/<i>yw</i>; +; UAS-<i>Mcm10IR<sub>633-700</sub></i>/UAS-<i>GFP</i>; (D) <i>GMR</i>-GAL4/<i>yw</i>; +; UAS-<i>Mcm10IR<sub>633-700</sub></i>/UAS-<i>P35</i>. E, F, G, and H show enlarged images of A, B, C, and D, respectively. White bars indicate 50 μm. The flies were reared at 28°C.</p>", "links"=>[], "tags"=>["Biochemistry", "dna", "DNA replication", "Nucleic acids", "developmental biology", "genetics", "Molecular genetics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "drosophila", "Drosophila melanogaster", "Model organisms", "Animal models", "apoptosis", "inhibitor", "p35", "suppressed", "induced", "knockdown"], "article_id"=>979091, "categories"=>["Biological Sciences"], "users"=>["Nicole Vo", "Ayano Taga", "Yasuhiro Inaba", "Hideki Yoshida", "Sue Cotterill", "Masamitsu Yamaguchi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093450.g008", "stats"=>{"downloads"=>0, "page_views"=>13, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Expression_of_apoptosis_inhibitor_P35_suppressed_the_rough_eye_induced_by_knockdown_of_dMcm10_/979091", "title"=>"Expression of apoptosis inhibitor P35 suppressed the rough eye induced by knockdown of <i>dMcm10</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-31 02:47:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1440965"], "description"=>"<p>The eye imaginal discs were stained with anti-phospho H2AvD antibody (Green) (A, B, C). Also shown are merged images of the eye discs stained with DAPI for DNA (Blue) and anti-phospho H2AvD antibody (Green) (D, E, F). (A, D) <i>GMR</i>-GAL4/<i>yw</i>; +; +; (B, E) <i>GMR</i>-GAL4/<i>yw</i>; +; UAS-<i>Mcm10IR<sub>633-700</sub></i>/+; (C, F) <i>GMR</i>-GAL4/<i>yw</i>; +; UAS-<i>Mcm10IR<sub>633-700</sub></i>/UAS-<i>Mcm10IR<sub>633-700</sub></i>. The white arrowhead shows the morphogenetic furrow. The flies were reared at 28°C. Bars indicate 40 μm. (G) Quantification of phospho-H2AvD positive index in the posterior region of eye discs. There was an increase in the phospho-H2AvD positive signals with two copies of <i>dMcm10</i> dsRNA in the posterior region compared to control, <i>GMR</i>-GAL4 alone. One copy of the dsRNA also caused an increase in DNA damage signal, but not as much as for two copies. **p<0.01. The red border line indicates the posterior region. (a) indicates anterior, (p) indicates posterior.</p>", "links"=>[], "tags"=>["Biochemistry", "dna", "DNA replication", "Nucleic acids", "developmental biology", "genetics", "Molecular genetics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "drosophila", "Drosophila melanogaster", "Model organisms", "Animal models", "imaginal", "discs"], "article_id"=>979076, "categories"=>["Biological Sciences"], "users"=>["Nicole Vo", "Ayano Taga", "Yasuhiro Inaba", "Hideki Yoshida", "Sue Cotterill", "Masamitsu Yamaguchi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093450.g006", "stats"=>{"downloads"=>1, "page_views"=>11, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Knockdown_of_dMcm10_in_eye_imaginal_discs_induces_DNA_damage_/979076", "title"=>"Knockdown of <i>dMcm10</i> in eye imaginal discs induces DNA damage.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-31 02:47:43"}
  • {"files"=>["https://ndownloader.figshare.com/files/1440910"], "description"=>"<p>The salivary glands of third instar lavae of Canton S were stained with anti-dMcm10 antibody (Red) (A, B, C), with anti-dpolα antibody (Green) (D, E, F) and with DAPI for DNA staining (Blue) (J, K, L). Merged images for anti-dMcm10 antibody and anti-dpolα antibody (G, H, I), and anti-dMcm10 and DAPI (M, N, O) are also shown. B, E, H, K, N and C, F, I, L, O show sequentially enlarged images of A, D, G, J, and M, respectively. Bars indicate 80 μm, 40 μm, 10 μm respectively. White arrowheads indicate the heterochromatic chromocenter.</p>", "links"=>[], "tags"=>["Biochemistry", "dna", "DNA replication", "Nucleic acids", "developmental biology", "genetics", "Molecular genetics", "organisms", "animals", "invertebrates", "arthropoda", "insects", "drosophila", "Drosophila melanogaster", "Model organisms", "Animal models", "localizes", "nucleus", "salivary", "gland"], "article_id"=>979041, "categories"=>["Biological Sciences"], "users"=>["Nicole Vo", "Ayano Taga", "Yasuhiro Inaba", "Hideki Yoshida", "Sue Cotterill", "Masamitsu Yamaguchi"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0093450.g001", "stats"=>{"downloads"=>0, "page_views"=>6, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mcm10_localizes_mainly_in_the_nucleus_of_salivary_gland_cells_/979041", "title"=>"Mcm10 localizes mainly in the nucleus of salivary gland cells.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-03-31 02:47:43"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"13", "full-text"=>"7", "pdf"=>"23", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"0", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"18", "full-text"=>"15", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"24", "supp-data"=>"7", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"7", "full-text"=>"8", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"10", "full-text"=>"8", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"9", "full-text"=>"10", "pdf"=>"4", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"0", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"7", "full-text"=>"3", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"10", "full-text"=>"7", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"16", "full-text"=>"12", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"1", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"7", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"5", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"9"}
  • {"unique-ip"=>"10", "full-text"=>"9", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"8", "full-text"=>"12", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"1", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"2", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"3", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"6", "full-text"=>"7", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[291]}, {"subject_area"=>"/Biology and life sciences/Anatomy", "average_usage"=>[267]}, {"subject_area"=>"/Biology and life sciences/Biochemistry", "average_usage"=>[282]}, {"subject_area"=>"/Biology and life sciences/Cell biology", "average_usage"=>[286]}, {"subject_area"=>"/Biology and life sciences/Developmental biology", "average_usage"=>[285]}, {"subject_area"=>"/Biology and life sciences/Organisms", "average_usage"=>[310]}]}
Loading … Spinner
There are currently no alerts