The KIT Gene Is Associated with the English Spotting Coat Color Locus and Congenital Megacolon in Checkered Giant Rabbits (Oryctolagus cuniculus)
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{"title"=>"The kit gene is associated with the english spotting coat color locus and congenital megacolon in checkered giant rabbits (oryctolagus cuniculus)", "type"=>"journal", "authors"=>[{"first_name"=>"Luca", "last_name"=>"Fontanesi", "scopus_author_id"=>"12807457900"}, {"first_name"=>"Manuela", "last_name"=>"Vargiolu", "scopus_author_id"=>"14826073500"}, {"first_name"=>"Emilio", "last_name"=>"Scotti", "scopus_author_id"=>"22939088000"}, {"first_name"=>"Rocco", "last_name"=>"Latorre", "scopus_author_id"=>"36461341000"}, {"first_name"=>"Maria Simonetta Faussone", "last_name"=>"Pellegrini", "scopus_author_id"=>"56145561700"}, {"first_name"=>"Maurizio", "last_name"=>"Mazzoni", "scopus_author_id"=>"12806758500"}, {"first_name"=>"Martina", "last_name"=>"Asti", "scopus_author_id"=>"56145364000"}, {"first_name"=>"Roberto", "last_name"=>"Chiocchetti", "scopus_author_id"=>"6602402979"}, {"first_name"=>"Giovanni", "last_name"=>"Romeo", "scopus_author_id"=>"7202071987"}, {"first_name"=>"Paolo", "last_name"=>"Clavenzani", "scopus_author_id"=>"6602984512"}, {"first_name"=>"Roberto", "last_name"=>"De Giorgio", "scopus_author_id"=>"7005471207"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"pui"=>"372990945", "pmid"=>"24736498", "doi"=>"10.1371/journal.pone.0093750", "scopus"=>"2-s2.0-84899693073", "issn"=>"19326203", "sgr"=>"84899693073"}, "id"=>"fc78ad5d-ec63-3b38-b547-ad13f87318cd", "abstract"=>"The English spotting coat color locus in rabbits, also known as Dominant white spotting locus, is determined by an incompletely dominant allele (En). Rabbits homozygous for the recessive wild-type allele (en/en) are self-colored, heterozygous En/en rabbits are normally spotted, and homozygous En/En animals are almost completely white. Compared to vital en/en and En/en rabbits, En/En animals are subvital because of a dilated (\"mega\") cecum and ascending colon. In this study, we investigated the role of the KIT gene as a candidate for the English spotting locus in Checkered Giant rabbits and characterized the abnormalities affecting enteric neurons and c-kit positive interstitial cells of Cajal (ICC) in the megacolon of En/En rabbits. Twenty-one litters were obtained by crossing three Checkered Giant bucks (En/en) with nine Checkered Giant (En/en) and two en/en does, producing a total of 138 F1 and backcrossed rabbits. Resequencing all coding exons and portions of non-coding regions of the KIT gene in 28 rabbits of different breeds identified 98 polymorphisms. A single nucleotide polymorphism genotyped in all F1 families showed complete cosegregation with the English spotting coat color phenotype (θ=0.00 LOD  =75.56). KIT gene expression in cecum and colon specimens of En/En (pathological) rabbits was 5-10% of that of en/en (control) rabbits. En/En rabbits showed reduced and altered c-kit immunolabelled ICC compared to en/en controls. Morphometric data on whole mounts of the ascending colon showed a significant decrease of HuC/D (P<0.05) and substance P (P<0.01) immunoreactive neurons in En/En vs. en/en. Electron microscopy analysis showed neuronal and ICC abnormalities in En/En tissues. The En/En rabbit model shows neuro-ICC changes reminiscent of the human non-aganglionic megacolon. This rabbit model may provide a better understanding of the molecular abnormalities underlying conditions associated with non-aganglionic megacolon.", "link"=>"http://www.mendeley.com/research/kit-gene-associated-english-spotting-coat-color-locus-congenital-megacolon-checkered-giant-rabbits-o", "reader_count"=>10, "reader_count_by_academic_status"=>{"Unspecified"=>2, "Researcher"=>3, "Student > Ph. D. Student"=>1, "Student > Postgraduate"=>1, "Student > Master"=>3}, "reader_count_by_user_role"=>{"Unspecified"=>2, "Researcher"=>3, "Student > Ph. D. Student"=>1, "Student > Postgraduate"=>1, "Student > Master"=>3}, "reader_count_by_subject_area"=>{"Unspecified"=>2, "Agricultural and Biological Sciences"=>6, "Veterinary Science and Veterinary Medicine"=>1, "Chemistry"=>1}, "reader_count_by_subdiscipline"=>{"Chemistry"=>{"Chemistry"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>6}, "Unspecified"=>{"Unspecified"=>2}, "Veterinary Science and Veterinary Medicine"=>{"Veterinary Science and Veterinary Medicine"=>1}}, "reader_count_by_country"=>{"United Kingdom"=>1}, "group_count"=>2}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1465537"], "description"=>"<p>Black squares and circles  =  rabbits with <i>en/en</i> genotype at the <i>English spotting</i> locus; spotted squares and circles  =  rabbits with <i>En/en</i> genotype; white squares and circles  =  rabbits with <i>En/En</i> genotype. ID of the animals is reported in blue or red (for those used as parental animals). The genotype of the g.93948587T>C SNP is reported for all animals. Rabbits that are circled in green have been used to collect specimens for anatomo-histochemical, gene expression analyses and resequencing (rabbits with n. 1–9). Rabbits that are circled in orange have been inspected after slaughtering. Animals with underlined ID showed hard feces in the intestine.</p>", "links"=>[], "tags"=>["genetics", "Animal genetics", "Molecular genetics", "backcrossed", "checkered"], "article_id"=>1000198, "categories"=>["Biological Sciences"], "users"=>["Luca Fontanesi", "Manuela Vargiolu", "Emilio Scotti", "Rocco Latorre", "Maria Simonetta Faussone Pellegrini", "Maurizio Mazzoni", "Martina Asti", "Roberto Chiocchetti", "Giovanni Romeo", "Paolo Clavenzani", "Roberto De Giorgio"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0093750.g001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_F1_and_backcrossed_families_obtained_from_Checkered_Giant_parental_animals_/1000198", "title"=>"F1 and backcrossed families obtained from Checkered Giant parental animals.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-15 03:55:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1465538"], "description"=>"<p>Macroscopic features of the gut in the rabbit model investigated in this study. Note the markedly dilated ascending colon in <i>En/En</i> (pathological) compared to <i>en/en</i> rabbits (control).</p>", "links"=>[], "tags"=>["genetics", "Animal genetics", "Molecular genetics", "intestine", "weaned"], "article_id"=>1000199, "categories"=>["Biological Sciences"], "users"=>["Luca Fontanesi", "Manuela Vargiolu", "Emilio Scotti", "Rocco Latorre", "Maria Simonetta Faussone Pellegrini", "Maurizio Mazzoni", "Martina Asti", "Roberto Chiocchetti", "Giovanni Romeo", "Paolo Clavenzani", "Roberto De Giorgio"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0093750.g002"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Typical_appearance_of_the_intestine_in_En_En_and_en_en_weaned_rabbits_/1000199", "title"=>"Typical appearance of the intestine in <i>En/En</i> and <i>en/en</i> weaned rabbits.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-15 03:55:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1465540"], "description"=>"<p>The genotypes are identified by the three coat color phenotypes, respectively.</p>", "links"=>[], "tags"=>["genetics", "Animal genetics", "Molecular genetics", "genotypes"], "article_id"=>1000201, "categories"=>["Biological Sciences"], "users"=>["Luca Fontanesi", "Manuela Vargiolu", "Emilio Scotti", "Rocco Latorre", "Maria Simonetta Faussone Pellegrini", "Maurizio Mazzoni", "Martina Asti", "Roberto Chiocchetti", "Giovanni Romeo", "Paolo Clavenzani", "Roberto De Giorgio"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0093750.g003"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Rabbits_with_En_En_En_en_and_en_en_genotypes_at_the_English_spotting_locus_/1000201", "title"=>"Rabbits with <i>En/En</i>, <i>En/en</i> and <i>en/en</i> genotypes at the <i>English spotting</i> locus.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-15 03:55:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1465541"], "description"=>"<p>In <i>En/En</i> rabbits <i>KIT</i> expression was significantly lower than in <i>en/en</i> rabbits (5±2%; <i>P</i><0.00001, <i>t</i>-test).</p>", "links"=>[], "tags"=>["genetics", "Animal genetics", "Molecular genetics", "ascending", "colon"], "article_id"=>1000202, "categories"=>["Biological Sciences"], "users"=>["Luca Fontanesi", "Manuela Vargiolu", "Emilio Scotti", "Rocco Latorre", "Maria Simonetta Faussone Pellegrini", "Maurizio Mazzoni", "Martina Asti", "Roberto Chiocchetti", "Giovanni Romeo", "Paolo Clavenzani", "Roberto De Giorgio"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0093750.g004"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Relative_KIT_gene_expression_levels_in_the_ascending_colon_of_En_En_and_en_en_rabbits_/1000202", "title"=>"Relative <i>KIT</i> gene expression levels in the ascending colon of <i>En/En</i> and <i>en/en</i> rabbits.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-15 03:55:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1465543"], "description"=>"<p><b>A</b>) <i>En/En</i>; <b>B</b>) <i>en/en</i>. The arrow indicate c-kit immunoreactivity in the <i>tunica muscularis</i> of the ascending colon in an <i>en/en</i> rabbit.</p>", "links"=>[], "tags"=>["genetics", "Animal genetics", "Molecular genetics", "images", "colonic", "mucosa"], "article_id"=>1000204, "categories"=>["Biological Sciences"], "users"=>["Luca Fontanesi", "Manuela Vargiolu", "Emilio Scotti", "Rocco Latorre", "Maria Simonetta Faussone Pellegrini", "Maurizio Mazzoni", "Martina Asti", "Roberto Chiocchetti", "Giovanni Romeo", "Paolo Clavenzani", "Roberto De Giorgio"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0093750.g005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Representative_images_of_rabbit_colonic_mucosa_in_En_En_vs_en_en_/1000204", "title"=>"Representative images of rabbit colonic mucosa in <i>En/En</i> vs <i>en/en</i>.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-15 03:55:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1465546"], "description"=>"<p><b>A</b>) E<i>n/En</i> myenteric ganglia (arrows) with human neuronal protein immunoreactive (HuC/D-IR) neurons. <b>B</b>) <i>En/En</i> myenteric ganglia (arrows) with nitric oxide synthase immunoreactive (nNOS-IR) neurons, (arrowheads) nNOS-IR nerve bundles. <b>C</b>) <i>en/en</i> myenteric ganglia (arrows) with HuC/D-IR neurons. <b>D</b>) e<i>n/en</i> myenteric ganglia (arrows) with nNOS-IR neurons, (arrowheads) nNOS-IR nerve bundles arranged within primary, secondary and tertiary nerve strands. In <i>En/En</i> (<b>A</b> and <b>B</b>) the ganglia and nerve bundles are less dense, have a lower number of HuC/D-IR and nNOS-IR neurons compared to <i>en/en</i> rabbits (<b>C</b> and <b>D</b>). <b>E</b>) and <b>F</b>): differences between <i>En/En</i> vs <i>en/en</i> SP-IR neurons (arrow), ganglia and nerve bundles (arrowhead) in the morphology of myenteric plexus in the ascending colon. In <i>En/En</i> (<b>E</b>) ganglia (arrow) are small and have a lower number of substance P immunoreactive (SP-IR) neurons than <i>en/en</i> rabbits (<b>F</b>). <b>A–D </b><i>scale bars</i> 200 µm; <b>E–F </b><i>scale bars</i> 100 µm.</p>", "links"=>[], "tags"=>["genetics", "Animal genetics", "Molecular genetics", "images", "colon", "mounts", "myenteric"], "article_id"=>1000207, "categories"=>["Biological Sciences"], "users"=>["Luca Fontanesi", "Manuela Vargiolu", "Emilio Scotti", "Rocco Latorre", "Maria Simonetta Faussone Pellegrini", "Maurizio Mazzoni", "Martina Asti", "Roberto Chiocchetti", "Giovanni Romeo", "Paolo Clavenzani", "Roberto De Giorgio"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0093750.g006"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Representative_images_of_colon_whole_mounts_in_En_En_and_en_en_rabbit_myenteric_plexuses_/1000207", "title"=>"Representative images of colon whole mounts in <i>En/En</i> and <i>en/en</i> rabbit myenteric plexuses.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-15 03:55:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1465548"], "description"=>"<p>Different immunoreactive neurons are reported: human neuronal protein (HuC/D) (<b>A</b>: <i>En/En</i> vs <i>en/en</i>, <i>P</i><0.05), nitric oxide synthase (nNOS) (<b>B</b>) and substance P (SP) (<b>C</b>: <i>En/En</i> vs <i>en/en</i>, <i>P</i><0.01) myenteric labeled neurons. Results are expressed as mean ± standard error of the mean.</p>", "links"=>[], "tags"=>["genetics", "Animal genetics", "Molecular genetics", "immunoreactive", "myenteric", "neurons"], "article_id"=>1000209, "categories"=>["Biological Sciences"], "users"=>["Luca Fontanesi", "Manuela Vargiolu", "Emilio Scotti", "Rocco Latorre", "Maria Simonetta Faussone Pellegrini", "Maurizio Mazzoni", "Martina Asti", "Roberto Chiocchetti", "Giovanni Romeo", "Paolo Clavenzani", "Roberto De Giorgio"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0093750.g007"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Mean_density_of_immunoreactive_myenteric_neurons_in_En_En_vs_en_en_rabbits_/1000209", "title"=>"Mean density of immunoreactive myenteric neurons in <i>En/En</i> vs <i>en/en</i> rabbits.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-15 03:55:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1465553"], "description"=>"<p><i>en/en</i> (<b>A</b> and <b>B</b>) control and <i>En/En</i> (<b>C</b> and <b>D</b>) pathological animals. <b>A</b>) ascending colon: an intramuscular ICC close to a nerve bundle (NB) and smooth muscle cells (SMC). Arrows indicate cell-to-cell contacts between ICC and SMC. x12,500. <b>B</b>) ascending colon: ICC at the submucosal border of the circular muscle layer showing in the cytoplasm several cisternae of the smooth endoplasmic reticulum and filaments, and caveolae along the plasma membrane; on the upper side, a nerve bundle and, on the lower side, the smooth muscle cells (SMC). x12,500. <b>C</b>) ascending colon: an intramuscular ICC with swollen mitochondria and extremely dilated cisternae of rough endoplasmic reticulum; on the upper side, a blood capillary (BC); on the right side, a nerve bundle (NB). The arrowhead indicates a nerve ending near the ICC. x15.000. <b>D</b>) descending colon: several intramuscular ICC with large intracytoplasmatic vacuoles; the arrows indicate cell-to-cell contacts between ICC and SMC. x7,500.</p>", "links"=>[], "tags"=>["genetics", "Animal genetics", "Molecular genetics", "microscopy", "interstitial", "cells", "cajal"], "article_id"=>1000214, "categories"=>["Biological Sciences"], "users"=>["Luca Fontanesi", "Manuela Vargiolu", "Emilio Scotti", "Rocco Latorre", "Maria Simonetta Faussone Pellegrini", "Maurizio Mazzoni", "Martina Asti", "Roberto Chiocchetti", "Giovanni Romeo", "Paolo Clavenzani", "Roberto De Giorgio"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0093750.g008"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Electron_microscopy_of_Interstitial_cells_of_Cajal_ICC_/1000214", "title"=>"Electron microscopy of Interstitial cells of Cajal (ICC).", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-15 03:55:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1465558"], "description"=>"<p><i>en/en</i> control (<b>A</b>) and <i>En/En</i> (<b>B</b> and <b>C</b>) pathological animals. <b>A</b>) descending colon: the neuron has an extended Golgi apparatus (G), thin RER cisternae, free ribosomes, and small oval shaped mitochondria. x10,000. <b>B</b>) descending colon: detail of the cytoplasm of a neuron with dilated RER cisternae and swollen mitochondria; G: Golgi apparatus. x15,000. <b>C</b>) ascending colon: a myenteric ganglion with three cells identifiable as neuroblasts (Nbl). These cells are small and have a scarce cytoplasm mainly containing free ribosomes. Glia: glial cells. x10,000. Intragangliar nerve endings (NE): pathological animals. <b>D</b>) descending and <b>E</b>) ascending colon. Most of the NE has an anomalous content: few or abnormally featured synaptic vesicles, lamellar bodies, lysosomes. <b>D</b>) x15,000; <b>E</b>) x20,000.</p>", "links"=>[], "tags"=>["genetics", "Animal genetics", "Molecular genetics", "microscopy", "myenteric"], "article_id"=>1000219, "categories"=>["Biological Sciences"], "users"=>["Luca Fontanesi", "Manuela Vargiolu", "Emilio Scotti", "Rocco Latorre", "Maria Simonetta Faussone Pellegrini", "Maurizio Mazzoni", "Martina Asti", "Roberto Chiocchetti", "Giovanni Romeo", "Paolo Clavenzani", "Roberto De Giorgio"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0093750.g009"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Electron_microscopy_of_myenteric_neurons_/1000219", "title"=>"Electron microscopy of myenteric neurons.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-15 03:55:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1465560"], "description"=>"1<p>The number of rabbits with the different genotypes is reported.</p>", "links"=>[], "tags"=>["genetics", "Animal genetics", "Molecular genetics", "rabbits", "genotyped", "nucleotide"], "article_id"=>1000221, "categories"=>["Biological Sciences"], "users"=>["Luca Fontanesi", "Manuela Vargiolu", "Emilio Scotti", "Rocco Latorre", "Maria Simonetta Faussone Pellegrini", "Maurizio Mazzoni", "Martina Asti", "Roberto Chiocchetti", "Giovanni Romeo", "Paolo Clavenzani", "Roberto De Giorgio"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0093750.t001"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Breeds_and_rabbits_genotyped_for_the_KIT_g_93948587T_C_single_nucleotide_polymorphism_/1000221", "title"=>"Breeds and rabbits genotyped for the <i>KIT</i> g.93948587T>C single nucleotide polymorphism.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-04-15 03:55:13"}
  • {"files"=>["https://ndownloader.figshare.com/files/1465565", "https://ndownloader.figshare.com/files/1465566", "https://ndownloader.figshare.com/files/1465567", "https://ndownloader.figshare.com/files/1465568", "https://ndownloader.figshare.com/files/1465569"], "description"=>"<div><p>The <i>English spotting</i> coat color locus in rabbits, also known as <i>Dominant white spotting</i> locus, is determined by an incompletely dominant allele (<i>En</i>). Rabbits homozygous for the recessive wild-type allele (<i>en</i>/<i>en</i>) are self-colored, heterozygous <i>En/en</i> rabbits are normally spotted, and homozygous <i>En/En</i> animals are almost completely white. Compared to vital <i>en</i>/<i>en</i> and <i>En/en</i> rabbits, <i>En/En</i> animals are subvital because of a dilated (“mega”) cecum and ascending colon. In this study, we investigated the role of the <i>KIT</i> gene as a candidate for the <i>English spotting</i> locus in Checkered Giant rabbits and characterized the abnormalities affecting enteric neurons and c-kit positive interstitial cells of Cajal (ICC) in the megacolon of <i>En/En</i> rabbits. Twenty-one litters were obtained by crossing three Checkered Giant bucks (<i>En/en</i>) with nine Checkered Giant (<i>En/en</i>) and two <i>en/en</i> does, producing a total of 138 F1 and backcrossed rabbits. Resequencing all coding exons and portions of non-coding regions of the <i>KIT</i> gene in 28 rabbits of different breeds identified 98 polymorphisms. A single nucleotide polymorphism genotyped in all F1 families showed complete cosegregation with the <i>English spotting</i> coat color phenotype (<i>θ</i> = 0.00 LOD  = 75.56). <i>KIT</i> gene expression in cecum and colon specimens of <i>En/En</i> (pathological) rabbits was 5–10% of that of <i>en/en</i> (control) rabbits. <i>En/En</i> rabbits showed reduced and altered c-kit immunolabelled ICC compared to <i>en/en</i> controls. Morphometric data on whole mounts of the ascending colon showed a significant decrease of HuC/D (<i>P</i><0.05) and substance P (<i>P</i><0.01) immunoreactive neurons in <i>En/En</i> vs. <i>en/en</i>. Electron microscopy analysis showed neuronal and ICC abnormalities in <i>En/En</i> tissues. The <i>En/En</i> rabbit model shows neuro-ICC changes reminiscent of the human non-aganglionic megacolon. This rabbit model may provide a better understanding of the molecular abnormalities underlying conditions associated with non-aganglionic megacolon.</p></div>", "links"=>[], "tags"=>["genetics", "Animal genetics", "Molecular genetics", "locus", "congenital", "megacolon", "checkered", "rabbits"], "article_id"=>1000226, "categories"=>["Biological Sciences"], "users"=>["Luca Fontanesi", "Manuela Vargiolu", "Emilio Scotti", "Rocco Latorre", "Maria Simonetta Faussone Pellegrini", "Maurizio Mazzoni", "Martina Asti", "Roberto Chiocchetti", "Giovanni Romeo", "Paolo Clavenzani", "Roberto De Giorgio"], "doi"=>["https://dx.doi.org/10.1371/journal.pone.0093750.s001", "https://dx.doi.org/10.1371/journal.pone.0093750.s002", "https://dx.doi.org/10.1371/journal.pone.0093750.s003", "https://dx.doi.org/10.1371/journal.pone.0093750.s004", "https://dx.doi.org/10.1371/journal.pone.0093750.s005"], "stats"=>{"downloads"=>0, "page_views"=>0, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/The_KIT_Gene_Is_Associated_with_the_English_Spotting_Coat_Color_Locus_and_Congenital_Megacolon_in_Checkered_Giant_Rabbits_Oryctolagus_cuniculus_/1000226", "title"=>"The <i>KIT</i> Gene Is Associated with the <i>English Spotting</i> Coat Color Locus and Congenital Megacolon in Checkered Giant Rabbits (<i>Oryctolagus cuniculus</i>)", "pos_in_sequence"=>0, "defined_type"=>4, "published_date"=>"2014-04-15 03:55:13"}

PMC Usage Stats | Further Information

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