Crystal Structure of the Shrimp Proliferating Cell Nuclear Antigen: Structural Complementarity with WSSV DNA Polymerase PIP-Box
Publication Date
April 11, 2014
Journal
PLOS ONE
Authors
Jesus S. Carrasco Miranda, Alonso A. Lopez Zavala, Aldo A. Arvizu Flores, Karina D. Garcia Orozco, et al
Volume
9
Issue
4
Pages
e94369
DOI
https://dx.plos.org/10.1371/journal.pone.0094369
Publisher URL
http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0094369
PubMed
http://www.ncbi.nlm.nih.gov/pubmed/24728082
PubMed Central
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3984155
Europe PMC
http://europepmc.org/abstract/MED/24728082
Web of Science
000336736200067
Scopus
84899652912
Mendeley
http://www.mendeley.com/research/crystal-structure-shrimp-proliferating-cell-nuclear-antigen-structural-complementarity-wssv-dna-poly
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Mendeley | Further Information

{"title"=>"Crystal structure of the shrimp proliferating cell nuclear antigen: Structural complementarity with WSSV DNA polymerase PIP-Box", "type"=>"journal", "authors"=>[{"first_name"=>"Jesus S.", "last_name"=>"Carrasco-Miranda", "scopus_author_id"=>"55481919100"}, {"first_name"=>"Alonso A.", "last_name"=>"Lopez-Zavala", "scopus_author_id"=>"8713474800"}, {"first_name"=>"Aldo A.", "last_name"=>"Arvizu-Flores", "scopus_author_id"=>"57189383133"}, {"first_name"=>"Karina D.", "last_name"=>"Garcia-Orozco", "scopus_author_id"=>"56000839300"}, {"first_name"=>"Vivian", "last_name"=>"Stojanoff", "scopus_author_id"=>"6603679042"}, {"first_name"=>"Enrique", "last_name"=>"Rudiño-Piñera", "scopus_author_id"=>"6603272503"}, {"first_name"=>"Luis G.", "last_name"=>"Brieba", "scopus_author_id"=>"56000772000"}, {"first_name"=>"Rogerio R.", "last_name"=>"Sotelo-Mundo", "scopus_author_id"=>"6602179004"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"sgr"=>"84899652912", "doi"=>"10.1371/journal.pone.0094369", "pui"=>"372981429", "pmid"=>"24728082", "scopus"=>"2-s2.0-84899652912", "issn"=>"19326203", "isbn"=>"1932-6203"}, "id"=>"16d722d9-6125-3f6e-b745-e21cad0fbd1b", "abstract"=>"DNA replication requires processivity factors that allow replicative DNA polymerases to extend long stretches of DNA. Some DNA viruses encode their own replicative DNA polymerase, such as the white spot syndrome virus (WSSV) that infects decapod crustaceans but still require host replication accessory factors. We have determined by X-ray diffraction the three-dimensional structure of the Pacific white leg shrimp Litopenaeus vannamei Proliferating Cell Nuclear Antigen (LvPCNA). This protein is a member of the sliding clamp family of proteins, that binds DNA replication and DNA repair proteins through a motif called PIP-box (PCNA-Interacting Protein). The crystal structure of LvPCNA was refined to a resolution of 3 Å, and allowed us to determine the trimeric protein assembly and details of the interactions between PCNA and the DNA. To address the possible interaction between LvPCNA and the viral DNA polymerase, we docked a theoretical model of a PIP-box peptide from the WSSV DNA polymerase within LvPCNA crystal structure. The theoretical model depicts a feasible model of interaction between both proteins. The crystal structure of shrimp PCNA allows us to further understand the mechanisms of DNA replication processivity factors in non-model systems.", "link"=>"http://www.mendeley.com/research/crystal-structure-shrimp-proliferating-cell-nuclear-antigen-structural-complementarity-wssv-dna-poly", "reader_count"=>16, "reader_count_by_academic_status"=>{"Unspecified"=>1, "Librarian"=>2, "Student > Doctoral Student"=>1, "Researcher"=>1, "Student > Ph. D. Student"=>4, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>1, "Lecturer"=>1, "Professor"=>2}, "reader_count_by_user_role"=>{"Unspecified"=>1, "Librarian"=>2, "Student > Doctoral Student"=>1, "Researcher"=>1, "Student > Ph. D. Student"=>4, "Student > Master"=>2, "Other"=>1, "Student > Bachelor"=>1, "Lecturer"=>1, "Professor"=>2}, "reader_count_by_subject_area"=>{"Unspecified"=>3, "Biochemistry, Genetics and Molecular Biology"=>4, "Agricultural and Biological Sciences"=>4, "Medicine and Dentistry"=>1, "Physics and Astronomy"=>1, "Psychology"=>1, "Chemistry"=>1, "Social Sciences"=>1}, "reader_count_by_subdiscipline"=>{"Medicine and Dentistry"=>{"Medicine and Dentistry"=>1}, "Chemistry"=>{"Chemistry"=>1}, "Social Sciences"=>{"Social Sciences"=>1}, "Physics and Astronomy"=>{"Physics and Astronomy"=>1}, "Psychology"=>{"Psychology"=>1}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>4}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>4}, "Unspecified"=>{"Unspecified"=>3}}, "group_count"=>0}

Scopus | Further Information

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1461723"], "description"=>"<p>The figure shows the high identity and similitude of <i>L. vannamei</i> PCNA with other species. Important domains for PCNA-protein interactions: Central Loop, Inter-domain Connector Loop (IDCL) and C-terminal are in colored boxes (blue, red and green respectively) and tagged.</p>", "links"=>[], "tags"=>["Biochemistry", "Nucleic acids", "Nucleic acid components", "proteins", "DNA-binding proteins", "protein structure", "Biomacromolecule-ligand interactions", "dna", "biophysics", "Biophysical simulations", "Marine biology", "molecular biology", "Molecular complexes", "chemistry", "computational chemistry", "alignment"], "article_id"=>996827, "categories"=>["Biological Sciences"], "users"=>["Jesus S. Carrasco-Miranda", "Alonso A. Lopez-Zavala", "Aldo A. Arvizu-Flores", "Karina D. Garcia-Orozco", "Vivian Stojanoff", "Enrique Rudiño-Piñera", "Luis G. Brieba", "Rogerio R. Sotelo-Mundo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0094369.g002", "stats"=>{"downloads"=>3, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Amino_acid_sequence_alignment_of_PCNAs_/996827", "title"=>"Amino acid sequence alignment of PCNAs.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-11 03:34:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1461745"], "description"=>"<p>Lateral and frontal view of the theoretical model of the polymerase is depicted with DNA. The PIP box is shown in red. PCNA is shown as an orange ring.</p>", "links"=>[], "tags"=>["Biochemistry", "Nucleic acids", "Nucleic acid components", "proteins", "DNA-binding proteins", "protein structure", "Biomacromolecule-ligand interactions", "dna", "biophysics", "Biophysical simulations", "Marine biology", "molecular biology", "Molecular complexes", "chemistry", "computational chemistry", "lvpcna", "wssv"], "article_id"=>996849, "categories"=>["Biological Sciences"], "users"=>["Jesus S. Carrasco-Miranda", "Alonso A. Lopez-Zavala", "Aldo A. Arvizu-Flores", "Karina D. Garcia-Orozco", "Vivian Stojanoff", "Enrique Rudiño-Piñera", "Luis G. Brieba", "Rogerio R. Sotelo-Mundo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0094369.g006", "stats"=>{"downloads"=>0, "page_views"=>12, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Proposed_interaction_between_LvPCNA_and_WSSV_DNA_polymerase_/996849", "title"=>"Proposed interaction between LvPCNA and WSSV DNA polymerase.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-11 03:34:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1461730"], "description"=>"<p>The model shows the final seven poses for the PIP-box peptide (cartoon) docked into the binding site of <i>Lv</i>PCNA (surface representation). Tagged residues are from PCNA and form the cavity for peptide interaction. Side chains of the consensus PIP-box residues are shown as gray lines.</p>", "links"=>[], "tags"=>["Biochemistry", "Nucleic acids", "Nucleic acid components", "proteins", "DNA-binding proteins", "protein structure", "Biomacromolecule-ligand interactions", "dna", "biophysics", "Biophysical simulations", "Marine biology", "molecular biology", "Molecular complexes", "chemistry", "computational chemistry", "best-scoring", "pcna-wssv", "pip"], "article_id"=>996834, "categories"=>["Biological Sciences"], "users"=>["Jesus S. Carrasco-Miranda", "Alonso A. Lopez-Zavala", "Aldo A. Arvizu-Flores", "Karina D. Garcia-Orozco", "Vivian Stojanoff", "Enrique Rudiño-Piñera", "Luis G. Brieba", "Rogerio R. Sotelo-Mundo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0094369.g004", "stats"=>{"downloads"=>1, "page_views"=>26, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Aligment_of_best_scoring_models_of_PCNA_WSSV_PIP_box_complex_from_docking_/996834", "title"=>"Aligment of best-scoring models of PCNA-WSSV PIP box complex from docking.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-11 03:34:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1461743"], "description"=>"<p>In all figures the peptide was shorten to the consensus sequence GQHKILYYDIE that makes contact with <i>Lv</i>PCNA. Panel A shows a LigPlot where the peptide interacts with <i>Lv</i>PCNA through polar contacts (green dotted lines) and hydrophobic interaction (). Panel B shows a cartoon of the peptide (yellow) posed on a <i>Lv</i>PCNA monomer (blue), in red are identified the three region that participate in protein-protein interaction. In panel C, a surface image of <i>Lv</i>PCNA shows the hydrophobic pocket where the WSSV PIPbox-peptide (yellow) is attached. In panel D, residues that participate in <i>Lv</i>PCNA-peptide complex are tagged, side chains of residues from IDCL, Central Loop and C- terminal are green colored and the peptide residues are yellow colored.</p>", "links"=>[], "tags"=>["Biochemistry", "Nucleic acids", "Nucleic acid components", "proteins", "DNA-binding proteins", "protein structure", "Biomacromolecule-ligand interactions", "dna", "biophysics", "Biophysical simulations", "Marine biology", "molecular biology", "Molecular complexes", "chemistry", "computational chemistry", "bound", "wssv"], "article_id"=>996847, "categories"=>["Biological Sciences"], "users"=>["Jesus S. Carrasco-Miranda", "Alonso A. Lopez-Zavala", "Aldo A. Arvizu-Flores", "Karina D. Garcia-Orozco", "Vivian Stojanoff", "Enrique Rudiño-Piñera", "Luis G. Brieba", "Rogerio R. Sotelo-Mundo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0094369.g005", "stats"=>{"downloads"=>5, "page_views"=>8, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Model_of_Lv_PCNA_bound_to_WSSV_PIPbox_peptide_/996847", "title"=>"Model of <i>Lv</i>PCNA bound to WSSV PIPbox-peptide.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-11 03:34:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1461719"], "description"=>"<p>The PCNA molecule is arranged as homotrimer and each monomer is shown in different color. The most important parts for protein-protein interaction of each monomer: Interdomain Conecting Loop (IDCL), Central Loop and C-terminal are labeled.</p>", "links"=>[], "tags"=>["Biochemistry", "Nucleic acids", "Nucleic acid components", "proteins", "DNA-binding proteins", "protein structure", "Biomacromolecule-ligand interactions", "dna", "biophysics", "Biophysical simulations", "Marine biology", "molecular biology", "Molecular complexes", "chemistry", "computational chemistry"], "article_id"=>996823, "categories"=>["Biological Sciences"], "users"=>["Jesus S. Carrasco-Miranda", "Alonso A. Lopez-Zavala", "Aldo A. Arvizu-Flores", "Karina D. Garcia-Orozco", "Vivian Stojanoff", "Enrique Rudiño-Piñera", "Luis G. Brieba", "Rogerio R. Sotelo-Mundo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0094369.g001", "stats"=>{"downloads"=>2, "page_views"=>72, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Crystal_structure_of_Litopenaues_vannamei_PCNA_/996823", "title"=>"Crystal structure of <i>Litopenaues vannamei</i> PCNA.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-11 03:34:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1461746"], "description"=>"<p>Values in parenthesis represent the statistics at the highest resolution bin.</p>§<p>R<i><sub>meas</sub></i> is a redundancy-independent version of R<i><sub>symm</sub></i>, R<i><sub>meas</sub></i> = ∑<i><sub>h</sub></i> √n<i><sub>h</sub></i>/n<i><sub>h</sub></i>−1 ∑<sup>nh</sup><sub>i</sub>|Î<sub>h</sub>−I<sub>h,i</sub>|/∑<i><sub>h</sub></i> ∑<sup>nh</sup><sub>i</sub>I<sub>h,i</sub>, where Î<sub>h</sub> = 1/n<sub>h</sub> ∑<sup>nh</sup><sub>i</sub>I<sub>h,i</sub>.</p>", "links"=>[], "tags"=>["Biochemistry", "Nucleic acids", "Nucleic acid components", "proteins", "DNA-binding proteins", "protein structure", "Biomacromolecule-ligand interactions", "dna", "biophysics", "Biophysical simulations", "Marine biology", "molecular biology", "Molecular complexes", "chemistry", "computational chemistry", "refinement"], "article_id"=>996850, "categories"=>["Biological Sciences"], "users"=>["Jesus S. Carrasco-Miranda", "Alonso A. Lopez-Zavala", "Aldo A. Arvizu-Flores", "Karina D. Garcia-Orozco", "Vivian Stojanoff", "Enrique Rudiño-Piñera", "Luis G. Brieba", "Rogerio R. Sotelo-Mundo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0094369.t001", "stats"=>{"downloads"=>1, "page_views"=>10, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Data_reduction_and_refinement_statistics_from_Lv_PCNA_structure_/996850", "title"=>"Data reduction and refinement statistics from <i>Lv</i>PCNA structure.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-04-11 03:34:31"}
  • {"files"=>["https://ndownloader.figshare.com/files/1461727"], "description"=>"<p>The positively charged regions are colored in blue and negatively charged regions in red. The central channel is identified as a highly positive charged hole where the double-strand DNA can slide through it.</p>", "links"=>[], "tags"=>["Biochemistry", "Nucleic acids", "Nucleic acid components", "proteins", "DNA-binding proteins", "protein structure", "Biomacromolecule-ligand interactions", "dna", "biophysics", "Biophysical simulations", "Marine biology", "molecular biology", "Molecular complexes", "chemistry", "computational chemistry", "pcna"], "article_id"=>996831, "categories"=>["Biological Sciences"], "users"=>["Jesus S. Carrasco-Miranda", "Alonso A. Lopez-Zavala", "Aldo A. Arvizu-Flores", "Karina D. Garcia-Orozco", "Vivian Stojanoff", "Enrique Rudiño-Piñera", "Luis G. Brieba", "Rogerio R. Sotelo-Mundo"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0094369.g003", "stats"=>{"downloads"=>2, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Electrostatic_surface_view_of_PCNA_from_shrimp_Litopenaeus_vannamei_and_human_/996831", "title"=>"Electrostatic surface view of PCNA from shrimp <i>Litopenaeus vannamei</i> and human.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-11 03:34:31"}

PMC Usage Stats | Further Information

  • {"unique-ip"=>"18", "full-text"=>"12", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"12", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"5"}
  • {"unique-ip"=>"9", "full-text"=>"15", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"6"}
  • {"unique-ip"=>"19", "full-text"=>"17", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"13", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"4"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"5"}
  • {"unique-ip"=>"12", "full-text"=>"13", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"7", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"3"}
  • {"unique-ip"=>"5", "full-text"=>"4", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"6", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"8"}
  • {"unique-ip"=>"13", "full-text"=>"11", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"9"}
  • {"unique-ip"=>"8", "full-text"=>"7", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"10"}
  • {"unique-ip"=>"8", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"6", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"7"}
  • {"unique-ip"=>"7", "full-text"=>"9", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"8"}
  • {"unique-ip"=>"8", "full-text"=>"5", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"9"}
  • {"unique-ip"=>"21", "full-text"=>"14", "pdf"=>"7", "abstract"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"4", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"10"}
  • {"unique-ip"=>"9", "full-text"=>"6", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"2"}
  • {"unique-ip"=>"14", "full-text"=>"9", "pdf"=>"2", "abstract"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"11"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2014", "month"=>"12"}
  • {"unique-ip"=>"9", "full-text"=>"7", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"1"}
  • {"unique-ip"=>"19", "full-text"=>"16", "pdf"=>"9", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"2", "year"=>"2015", "month"=>"11"}
  • {"unique-ip"=>"9", "full-text"=>"5", "pdf"=>"5", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2015", "month"=>"12"}
  • {"unique-ip"=>"21", "full-text"=>"11", "pdf"=>"15", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"1"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"7", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"3"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"6"}
  • {"unique-ip"=>"8", "full-text"=>"4", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"2", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"7"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"8"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"4", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"10"}
  • {"unique-ip"=>"3", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"11"}
  • {"unique-ip"=>"6", "full-text"=>"5", "pdf"=>"2", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2016", "month"=>"12"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"2"}
  • {"unique-ip"=>"6", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"4"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"5"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"5", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"9"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"10"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"11"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"1", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2017", "month"=>"12"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"3", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"1"}
  • {"unique-ip"=>"5", "full-text"=>"3", "pdf"=>"3", "abstract"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"15", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"1"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"4"}
  • {"unique-ip"=>"3", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"5"}
  • {"unique-ip"=>"6", "full-text"=>"11", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"11"}
  • {"unique-ip"=>"3", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"6"}
  • {"unique-ip"=>"4", "full-text"=>"3", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"7"}
  • {"unique-ip"=>"2", "full-text"=>"1", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"1", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"10"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"1", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"9"}
  • {"unique-ip"=>"1", "full-text"=>"1", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"8"}
  • {"unique-ip"=>"5", "full-text"=>"6", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2018", "month"=>"12"}
  • {"unique-ip"=>"2", "full-text"=>"3", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"2"}
  • {"unique-ip"=>"4", "full-text"=>"4", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"3"}
  • {"unique-ip"=>"2", "full-text"=>"0", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"4"}
  • {"unique-ip"=>"7", "full-text"=>"7", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"5"}
  • {"unique-ip"=>"5", "full-text"=>"5", "pdf"=>"2", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"8"}
  • {"unique-ip"=>"2", "full-text"=>"2", "pdf"=>"0", "scanned-summary"=>"0", "scanned-page-browse"=>"0", "figure"=>"0", "supp-data"=>"0", "cited-by"=>"0", "year"=>"2019", "month"=>"9"}

Relative Metric

{"start_date"=>"2014-01-01T00:00:00Z", "end_date"=>"2014-12-31T00:00:00Z", "subject_areas"=>[{"subject_area"=>"/Biology and life sciences", "average_usage"=>[291]}, {"subject_area"=>"/Biology and life sciences/Molecular biology", "average_usage"=>[292, 461]}, {"subject_area"=>"/Physical sciences/Physics", "average_usage"=>[266]}]}
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