Comprehensive Analysis of Human Cells Motion under an Irrotational AC Electric Field in an Electro-Microfluidic Chip
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{"title"=>"Comprehensive analysis of human cells motion under an irrotational AC electric field in an electro-microfluidic chip", "type"=>"journal", "authors"=>[{"first_name"=>"Clarisse", "last_name"=>"Vaillier", "scopus_author_id"=>"56145101900"}, {"first_name"=>"Thibault", "last_name"=>"Honegger", "scopus_author_id"=>"25947525900"}, {"first_name"=>"Frédérique", "last_name"=>"Kermarrec", "scopus_author_id"=>"36096747000"}, {"first_name"=>"Xavier", "last_name"=>"Gidrol", "scopus_author_id"=>"6603490467"}, {"first_name"=>"David", "last_name"=>"Peyrade", "scopus_author_id"=>"6701501929"}], "year"=>2014, "source"=>"PLoS ONE", "identifiers"=>{"scopus"=>"2-s2.0-84929940157", "issn"=>"19326203", "pui"=>"607990632", "pmid"=>"24736275", "doi"=>"10.1371/journal.pone.0095231", "sgr"=>"84929940157"}, "id"=>"9fdd3173-cdbd-386b-968b-fc706c2b93ea", "abstract"=>"AC electrokinetics is a versatile tool for contact-less manipulation or characterization of cells and has been widely used for separation based on genotype translation to electrical phenotypes. Cells responses to an AC electric field result in a complex combination of electrokinetic phenomena, mainly dielectrophoresis and electrohydrodynamic forces. Human cells behaviors to AC electrokinetics remain unclear over a large frequency spectrum as illustrated by the self-rotation effect observed recently. We here report and analyze human cells behaviors in different conditions of medium conductivity, electric field frequency and magnitude. We also observe the self-rotation of human cells, in the absence of a rotational electric field. Based on an analytical competitive model of electrokinetic forces, we propose an explanation of the cell self-rotation. These experimental results, coupled with our model, lead to the exploitation of the cell behaviors to measure the intrinsic dielectric properties of JURKAT, HEK and PC3 human cell lines.", "link"=>"http://www.mendeley.com/research/comprehensive-analysis-human-cells-motion-under-irrotational-ac-electric-field-electromicrofluidic-c", "reader_count"=>20, "reader_count_by_academic_status"=>{"Professor > Associate Professor"=>1, "Student > Doctoral Student"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>1, "Other"=>2, "Student > Master"=>2, "Student > Bachelor"=>2, "Professor"=>2}, "reader_count_by_user_role"=>{"Professor > Associate Professor"=>1, "Student > Doctoral Student"=>1, "Researcher"=>3, "Student > Ph. D. Student"=>6, "Student > Postgraduate"=>1, "Other"=>2, "Student > Master"=>2, "Student > Bachelor"=>2, "Professor"=>2}, "reader_count_by_subject_area"=>{"Engineering"=>9, "Biochemistry, Genetics and Molecular Biology"=>2, "Agricultural and Biological Sciences"=>5, "Physics and Astronomy"=>2, "Chemical Engineering"=>1, "Computer Science"=>1}, "reader_count_by_subdiscipline"=>{"Engineering"=>{"Engineering"=>9}, "Physics and Astronomy"=>{"Physics and Astronomy"=>2}, "Agricultural and Biological Sciences"=>{"Agricultural and Biological Sciences"=>5}, "Computer Science"=>{"Computer Science"=>1}, "Biochemistry, Genetics and Molecular Biology"=>{"Biochemistry, Genetics and Molecular Biology"=>2}, "Chemical Engineering"=>{"Chemical Engineering"=>1}}, "reader_count_by_country"=>{"Japan"=>1}, "group_count"=>1}

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Figshare

  • {"files"=>["https://ndownloader.figshare.com/files/1465207"], "description"=>"<p><b>(a1)</b> Time-lapse sequence images of the rotation of HEK cells in the z-axis, in presence of 1 µm polystyrene colloid (highlighted in blue circles). Particles were added to observe medium stream lines. The red circle pinpoints a visible organelle. Rotation is studied at σ<sub>m</sub>  =  2.10<sup>−2</sup> S/m when varying <b>(a2)</b> magnitude of the electric field at <i>f</i>  =  45 kHz or <b>(a3)</b> frequency at magnitude 0.065 V/µm (V = 10 Vp-p). The dashed line plots the values of |Re[CMF(ω)]| at the same frequencies, bringing out the relation between DEP effect and ETE. Rotation studies of <b>(b)</b> of JURKAT cells and <b>(c)</b> PC3 cells (electric field magnitude is 0.089 V/µm (V = 4Vp-p) and σ<sub>m</sub>  =  2 10<sup>−2</sup> S/m.). The inset on the lower part of the graphs shows the number of cells used for each mean value.</p>", "links"=>[], "tags"=>["biophysics", "cell biology", "Cell physiology", "Membrane trafficking", "Cell processes", "Cellular stress responses", "cytometry", "Molecular cell biology", "Mechanical engineering", "physics", "Classical mechanics", "Continuum mechanics", "fluid mechanics", "electricity", "electrostatics"], "article_id"=>999936, "categories"=>["Biological Sciences"], "users"=>["Clarisse Vaillier", "Thibault Honegger", "Frédérique Kermarrec", "Xavier Gidrol", "David Peyrade"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095231.g006", "stats"=>{"downloads"=>0, "page_views"=>3, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Rotation_study_of_three_human_cell_lines_/999936", "title"=>"Rotation study of three human cell lines.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-15 03:33:23"}
  • {"files"=>["https://ndownloader.figshare.com/files/1465205"], "description"=>"<p>nDEP and pDEP are applied at <i>f</i>  =  1 kHz and <i>f</i>  =  200 kHz, respectively. The arrow represents cell motion during 5 frames (300 ms), the picture being the last image. DEP is stronger at low conductivities compared to EHD forces so cells experience larger displacement at higher velocities at low conductivities.</p>", "links"=>[], "tags"=>["biophysics", "cell biology", "Cell physiology", "Membrane trafficking", "Cell processes", "Cellular stress responses", "cytometry", "Molecular cell biology", "Mechanical engineering", "physics", "Classical mechanics", "Continuum mechanics", "fluid mechanics", "electricity", "electrostatics", "hek", "cells", "dielectrophoresis"], "article_id"=>999934, "categories"=>["Biological Sciences"], "users"=>["Clarisse Vaillier", "Thibault Honegger", "Frédérique Kermarrec", "Xavier Gidrol", "David Peyrade"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095231.g005", "stats"=>{"downloads"=>1, "page_views"=>16, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Response_of_HEK_cells_to_dielectrophoresis_for_increasing_medium_conductivities_/999934", "title"=>"Response of HEK cells to dielectrophoresis for increasing medium conductivities.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-15 03:33:23"}
  • {"files"=>["https://ndownloader.figshare.com/files/1465203"], "description"=>"<p>Cells are dragged in bulk rolls and the membrane rapidly breaks.</p>", "links"=>[], "tags"=>["biophysics", "cell biology", "Cell physiology", "Membrane trafficking", "Cell processes", "Cellular stress responses", "cytometry", "Molecular cell biology", "Mechanical engineering", "physics", "Classical mechanics", "Continuum mechanics", "fluid mechanics", "electricity", "electrostatics", "images", "aceo", "ete", "hek", "cells", "taken", "microfluidi", "schema", "observed"], "article_id"=>999932, "categories"=>["Biological Sciences"], "users"=>["Clarisse Vaillier", "Thibault Honegger", "Frédérique Kermarrec", "Xavier Gidrol", "David Peyrade"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095231.g004", "stats"=>{"downloads"=>0, "page_views"=>19, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Time_lapse_sequence_images_of_cell_destruction_in_conditions_of_dominant_ACEO_and_ETE_f__1_kHz_V_pp_8202_8202_8_V_963_m_8202_8202_2_10_8722_4_S_m_a_picture_of_HEK_cells_taken_in_a_microfluidi_chip_and_b_schema_of_the_observed_motions_/999932", "title"=>"Time-lapse sequence images of cell destruction in conditions of dominant ACEO and ETE (<i>f</i>  =  1 kHz, V<sub>pp</sub>  =  8 V, σ<sub>m</sub>  =  2 10<sup>−4</sup> S/m) (a) picture of HEK cells taken in a microfluidi chip and (b) schema of the observed motions.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-15 03:33:23"}
  • {"files"=>["https://ndownloader.figshare.com/files/1465197"], "description"=>"<p>Photographs and schemes illustrate the cell motion for typical frequencies and magnitudes with corresponding graphs of the DEP (U<sub>DEP</sub>, green line) and EHD (U<sub>EHD</sub>, in red) velocities. The velocities were calculated according to the theoretical model presented in the first paragraph and position of the field was taken for x = 1 µm. The boxed text refers to the related paragraph.</p>", "links"=>[], "tags"=>["biophysics", "cell biology", "Cell physiology", "Membrane trafficking", "Cell processes", "Cellular stress responses", "cytometry", "Molecular cell biology", "Mechanical engineering", "physics", "Classical mechanics", "Continuum mechanics", "fluid mechanics", "electricity", "electrostatics", "cells", "behaviors"], "article_id"=>999926, "categories"=>["Biological Sciences"], "users"=>["Clarisse Vaillier", "Thibault Honegger", "Frédérique Kermarrec", "Xavier Gidrol", "David Peyrade"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095231.g003", "stats"=>{"downloads"=>1, "page_views"=>5, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Summary_of_cells_behaviors_at_a_m_8202_8202_2_10_8722_4_S_m_and_b_963_m_8202_8202_2_10_8722_2_S_m_/999926", "title"=>"Summary of cells behaviors at (a) σ<sub>m</sub>  =  2.10<sup>−4</sup> S/m and (b) σ<sub>m</sub>  =  2.10<sup>−2</sup> S/m.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-15 03:33:23"}
  • {"files"=>["https://ndownloader.figshare.com/files/1465194"], "description"=>"<p><b>(a.1)</b> Plot of the real part of the Clausius-Mossotti factor for human cells with single shell models (parameters extracted respectively from <a href=\"http://www.plosone.org/article/info:doi/10.1371/journal.pone.0095231#pone.0095231-Huang1\" target=\"_blank\">[27]</a> for HeLa-60) and σ<sub>m</sub>  = 2.10−2 S/m, <b>(b.1)</b> Plot of the ACEO mean velocities of the fluid near the electrodes (x = 1 µm) for several conductivities of the fluidic medium for water (ε<sub>f</sub> = 78) and <b>(c.1)</b> Plot of the Π factor as a function of frequency for several conductivities of the medium. Review of predominant forces in presence of AC electric field and the induced motion of liquid and cells: <b>(a.2)</b> Dielectrophoresis (DEP) induces attraction (p-DEP) or repelling (n-DEP) of cells from high field region (in co-planar cases, electrode edges), <b>(b.2)</b> AC electroosmosis (ACEO) are electrohydrodynamic forces that create convective rolls over the electrodes edges and drag cell with them and <b>(c.2)</b> electrothermal effects (ETE).</p>", "links"=>[], "tags"=>["biophysics", "cell biology", "Cell physiology", "Membrane trafficking", "Cell processes", "Cellular stress responses", "cytometry", "Molecular cell biology", "Mechanical engineering", "physics", "Classical mechanics", "Continuum mechanics", "fluid mechanics", "electricity", "electrostatics", "streamlines", "induced", "ac", "electrokinetic"], "article_id"=>999923, "categories"=>["Biological Sciences"], "users"=>["Clarisse Vaillier", "Thibault Honegger", "Frédérique Kermarrec", "Xavier Gidrol", "David Peyrade"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095231.g002", "stats"=>{"downloads"=>0, "page_views"=>18, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Plots_of_critical_parameters_and_streamlines_induced_by_AC_electrokinetic_forces_/999923", "title"=>"Plots of critical parameters and streamlines induced by AC electrokinetic forces.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-15 03:33:23"}
  • {"files"=>["https://ndownloader.figshare.com/files/1465211"], "description"=>"<div><p>AC electrokinetics is a versatile tool for contact-less manipulation or characterization of cells and has been widely used for separation based on genotype translation to electrical phenotypes. Cells responses to an AC electric field result in a complex combination of electrokinetic phenomena, mainly dielectrophoresis and electrohydrodynamic forces. Human cells behaviors to AC electrokinetics remain unclear over a large frequency spectrum as illustrated by the self-rotation effect observed recently. We here report and analyze human cells behaviors in different conditions of medium conductivity, electric field frequency and magnitude. We also observe the self-rotation of human cells, in the absence of a rotational electric field. Based on an analytical competitive model of electrokinetic forces, we propose an explanation of the cell self-rotation. These experimental results, coupled with our model, lead to the exploitation of the cell behaviors to measure the intrinsic dielectric properties of JURKAT, HEK and PC3 human cell lines.</p></div>", "links"=>[], "tags"=>["biophysics", "cell biology", "Cell physiology", "Membrane trafficking", "Cell processes", "Cellular stress responses", "cytometry", "Molecular cell biology", "Mechanical engineering", "physics", "Classical mechanics", "Continuum mechanics", "fluid mechanics", "electricity", "electrostatics", "cells", "irrotational", "ac", "electro-microfluidic"], "article_id"=>999940, "categories"=>["Biological Sciences"], "users"=>["Clarisse Vaillier", "Thibault Honegger", "Frédérique Kermarrec", "Xavier Gidrol", "David Peyrade"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095231", "stats"=>{"downloads"=>0, "page_views"=>4, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Comprehensive_Analysis_of_Human_Cells_Motion_under_an_Irrotational_AC_Electric_Field_in_an_Electro_Microfluidic_Chip_/999940", "title"=>"Comprehensive Analysis of Human Cells Motion under an Irrotational AC Electric Field in an Electro-Microfluidic Chip", "pos_in_sequence"=>0, "defined_type"=>2, "published_date"=>"2014-04-15 03:33:23"}
  • {"files"=>["https://ndownloader.figshare.com/files/1465208"], "description"=>"<p>The cytoplasm conductivity σ<sub>3</sub> and membrane capacitance <i>C<sub>mem</sub></i> are calculated from experimental fit to the competitive model.</p>", "links"=>[], "tags"=>["biophysics", "cell biology", "Cell physiology", "Membrane trafficking", "Cell processes", "Cellular stress responses", "cytometry", "Molecular cell biology", "Mechanical engineering", "physics", "Classical mechanics", "Continuum mechanics", "fluid mechanics", "electricity", "electrostatics", "dielectric"], "article_id"=>999937, "categories"=>["Biological Sciences"], "users"=>["Clarisse Vaillier", "Thibault Honegger", "Frédérique Kermarrec", "Xavier Gidrol", "David Peyrade"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095231.t001", "stats"=>{"downloads"=>0, "page_views"=>7, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Table_of_the_dielectric_parameters_for_three_human_cell_lines_/999937", "title"=>"Table of the dielectric parameters for three human cell lines.", "pos_in_sequence"=>0, "defined_type"=>3, "published_date"=>"2014-04-15 03:33:23"}
  • {"files"=>["https://ndownloader.figshare.com/files/1465193"], "description"=>"<p>Single shell model of a mamalian cell with dielectric parameters annoted.</p>", "links"=>[], "tags"=>["biophysics", "cell biology", "Cell physiology", "Membrane trafficking", "Cell processes", "Cellular stress responses", "cytometry", "Molecular cell biology", "Mechanical engineering", "physics", "Classical mechanics", "Continuum mechanics", "fluid mechanics", "electricity", "electrostatics", "mamalian", "dielectric"], "article_id"=>999922, "categories"=>["Biological Sciences"], "users"=>["Clarisse Vaillier", "Thibault Honegger", "Frédérique Kermarrec", "Xavier Gidrol", "David Peyrade"], "doi"=>"https://dx.doi.org/10.1371/journal.pone.0095231.g001", "stats"=>{"downloads"=>0, "page_views"=>47, "likes"=>0}, "figshare_url"=>"https://figshare.com/articles/_Single_shell_model_of_a_mamalian_cell_with_dielectric_parameters_annoted_/999922", "title"=>"Single shell model of a mamalian cell with dielectric parameters annoted.", "pos_in_sequence"=>0, "defined_type"=>1, "published_date"=>"2014-04-15 03:33:23"}

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